Cp4.1LG04g09910.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g09910.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionheat stress transcription factor C-1-like
LocationCp4.1LG04: 9776028 .. 9777332 (-)
Sequence length1155
RNA-Seq ExpressionCp4.1LG04g09910.1
SyntenyCp4.1LG04g09910.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TACGCGCGTCCATTATTTATTTACAGTGCTCCCTCATCTTTCCAAAAATTCTCCTCCGTCCGCAGCTGCTTCTCTAAAAAGCTTATGGGATGGACGCCAACAACGAGTCTGCGAACGACATTGTCGCCCCTTTTGTTCTGAAGACTTATCAGATGGTCAACGATCCCCTTACCGACACTTTCATTACTTGGGGCTCTGCCAATAACAGCTTCCTCGTCCTCGACCCTCTCCATTTCTCTCACACTCTCTTGCCTGCTTATTTTAAGCATAATAACTTCTCCAGCTTCGTCCGACAGCTCAATACATACGTATGTCATTTCCGATTTCCTTATTTGATTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTNTTTTTTTTTTTGCTGTTTTTTGATTGTGGGTTTTGTTTTGAGTAGGGATTTCGGAAGGTAGATCCCGATAGATGGGAATTTGCAAACCAGTGGTTTCTGCGAGACCAATTCCATTTACTCAGAAACATTGTCAGGAGAAAACAGAGTACGAATTCGAGCCTGAGAGAGATGGGGGACGAGGAATTGGTGACTGAGATTGCCAGGCTTAGAAAAGAACAGAAGGTTTTGGAAGAGGAGCTTGAAGGCATGAAAAAACGTCTCGAAACTACAGAGAAACGACCGCAACAAATGATGGCGTTTCTTCATAAAGTGGCGGAAGATCCTGAAATTCTGCCTCGGATGATGCTTCAGAAGGATCGACCCGGCCCTCATATTGGGGATAAGAAACGCCGATTGATGATTTCGTCTTCTTCCTCATCGCCATCCACCACAACGGTGACGACCACGTCCTTGGAGGCAGAGGAAATCGAGGAAGAGGGCAGCTGGCGGCCGAATTTTTCGTCGCCGGAGGTGTCGGGACCGGCGGGATTCTGTAATCACGTGCAGCGTTGGAATTTGGCGGAAGCGGAGGCGGGTGGAATGGATATAAGTAGAAGCAGAGGCGGCGGAGAGATGAAGAATTCGACGGAAATGATGGGAACCGTGGAGGAGAGTAATCCAGCACCGCCTTATCCGTTTTCTCTGTTCGGGTGTGGGTTTTAGTTAATTACTCCCATTTGATTCTCATCATTTCCTTCCTTCCTTCCTTCCTCGCATTTTATCATCTTTATTTTGTTTTCTTTTATTAATAATTCCTTCCACTTTCATTTCTTCTTTGAATTCTCCCTATACTATACATATATGAAAGAATCAAGAATCATGGCTCTTGTATTTCCCCATCATTTATCATCTT

mRNA sequence

TACGCGCGTCCATTATTTATTTACAGTGCTCCCTCATCTTTCCAAAAATTCTCCTCCGTCCGCAGCTGCTTCTCTAAAAAGCTTATGGGATGGACGCCAACAACGAGTCTGCGAACGACATTGTCGCCCCTTTTGTTCTGAAGACTTATCAGATGGTCAACGATCCCCTTACCGACACTTTCATTACTTGGGGCTCTGCCAATAACAGCTTCCTCGTCCTCGACCCTCTCCATTTCTCTCACACTCTCTTGCCTGCTTATTTTAAGCATAATAACTTCTCCAGCTTCGTCCGACAGCTCAATACATACGGATTTCGGAAGGTAGATCCCGATAGATGGGAATTTGCAAACCAGTGGTTTCTGCGAGACCAATTCCATTTACTCAGAAACATTGTCAGGAGAAAACAGAGTACGAATTCGAGCCTGAGAGAGATGGGGGACGAGGAATTGGTGACTGAGATTGCCAGGCTTAGAAAAGAACAGAAGGTTTTGGAAGAGGAGCTTGAAGGCATGAAAAAACGTCTCGAAACTACAGAGAAACGACCGCAACAAATGATGGCGTTTCTTCATAAAGTGGCGGAAGATCCTGAAATTCTGCCTCGGATGATGCTTCAGAAGGATCGACCCGGCCCTCATATTGGGGATAAGAAACGCCGATTGATGATTTCGTCTTCTTCCTCATCGCCATCCACCACAACGGTGACGACCACGTCCTTGGAGGCAGAGGAAATCGAGGAAGAGGGCAGCTGGCGGCCGAATTTTTCGTCGCCGGAGGTGTCGGGACCGGCGGGATTCTGTAATCACGTGCAGCGTTGGAATTTGGCGGAAGCGGAGGCGGGTGGAATGGATATAAGTAGAAGCAGAGGCGGCGGAGAGATGAAGAATTCGACGGAAATGATGGGAACCGTGGAGGAGAGTAATCCAGCACCGCCTTATCCGTTTTCTCTGTTCGGGTGTGGGTTTTAGTTAATTACTCCCATTTGATTCTCATCATTTCCTTCCTTCCTTCCTTCCTCGCATTTTATCATCTTTATTTTGTTTTCTTTTATTAATAATTCCTTCCACTTTCATTTCTTCTTTGAATTCTCCCTATACTATACATATATGAAAGAATCAAGAATCATGGCTCTTGTATTTCCCCATCATTTATCATCTT

Coding sequence (CDS)

ATGGACGCCAACAACGAGTCTGCGAACGACATTGTCGCCCCTTTTGTTCTGAAGACTTATCAGATGGTCAACGATCCCCTTACCGACACTTTCATTACTTGGGGCTCTGCCAATAACAGCTTCCTCGTCCTCGACCCTCTCCATTTCTCTCACACTCTCTTGCCTGCTTATTTTAAGCATAATAACTTCTCCAGCTTCGTCCGACAGCTCAATACATACGGATTTCGGAAGGTAGATCCCGATAGATGGGAATTTGCAAACCAGTGGTTTCTGCGAGACCAATTCCATTTACTCAGAAACATTGTCAGGAGAAAACAGAGTACGAATTCGAGCCTGAGAGAGATGGGGGACGAGGAATTGGTGACTGAGATTGCCAGGCTTAGAAAAGAACAGAAGGTTTTGGAAGAGGAGCTTGAAGGCATGAAAAAACGTCTCGAAACTACAGAGAAACGACCGCAACAAATGATGGCGTTTCTTCATAAAGTGGCGGAAGATCCTGAAATTCTGCCTCGGATGATGCTTCAGAAGGATCGACCCGGCCCTCATATTGGGGATAAGAAACGCCGATTGATGATTTCGTCTTCTTCCTCATCGCCATCCACCACAACGGTGACGACCACGTCCTTGGAGGCAGAGGAAATCGAGGAAGAGGGCAGCTGGCGGCCGAATTTTTCGTCGCCGGAGGTGTCGGGACCGGCGGGATTCTGTAATCACGTGCAGCGTTGGAATTTGGCGGAAGCGGAGGCGGGTGGAATGGATATAAGTAGAAGCAGAGGCGGCGGAGAGATGAAGAATTCGACGGAAATGATGGGAACCGTGGAGGAGAGTAATCCAGCACCGCCTTATCCGTTTTCTCTGTTCGGGTGTGGGTTTTAG

Protein sequence

MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEELVTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPGPHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQRWNLAEAEAGGMDISRSRGGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF
Homology
BLAST of Cp4.1LG04g09910.1 vs. ExPASy Swiss-Prot
Match: Q9LV52 (Heat stress transcription factor C-1 OS=Arabidopsis thaliana OX=3702 GN=HSFC1 PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 7.2e-68
Identity = 157/337 (46.59%), Postives = 200/337 (59.35%), Query Frame = 0

Query: 2   DANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHN 61
           ++NN + N+++APF++KTYQMVNDP TD  ITWG A+NSF+V+DPL FS  +LPAYFKHN
Sbjct: 5   NSNNNNNNNVIAPFIVKTYQMVNDPSTDWLITWGPAHNSFIVVDPLDFSQRILPAYFKHN 64

Query: 62  NFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEELV 121
           NFSSFVRQLNTYGFRKVDPDRWEFAN+ FLR Q HLL NI RRK +     +++ D E+V
Sbjct: 65  NFSSFVRQLNTYGFRKVDPDRWEFANEHFLRGQKHLLNNIARRKHARGMYGQDLEDGEIV 124

Query: 122 TEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDR--P 181
            EI RL++EQ+ LE E++ M +R+E TEKRP+QMMAFL+KV EDP++LPRMML+K+R   
Sbjct: 125 REIERLKEEQRELEAEIQRMNRRIEATEKRPEQMMAFLYKVVEDPDLLPRMMLEKERTKQ 184

Query: 182 GPHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEE--------GSWRPNFSSPE--- 241
              + DKK+R             T++T   E EE+EE+         S  P+ SS E   
Sbjct: 185 QQQVSDKKKR-----------RVTMSTVKSEEEEVEEDEGRVFRVMSSSTPSPSSTENLY 244

Query: 242 -VSGPAGFCNHVQRWNLAEAEAG----------------------GMDISRSRGGG---- 292
               P G+   + +      E G                       +  S + GGG    
Sbjct: 245 RNHSPDGWIVPMTQGQFGSYETGLVAKSMLSNSTSSTSSSLTSTFSLPESVNGGGGGGCG 304

BLAST of Cp4.1LG04g09910.1 vs. ExPASy Swiss-Prot
Match: Q6EUG4 (Heat stress transcription factor C-2a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFC2A PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.0e-45
Identity = 109/214 (50.93%), Postives = 138/214 (64.49%), Query Frame = 0

Query: 12  VAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFSSFVRQLN 71
           VAPFV KTY+MV+DP TD  I WG  +NSF+V DP  FS TLLPA+FKH+NFSSFVRQLN
Sbjct: 12  VAPFVAKTYRMVDDPATDGVIAWGRDSNSFVVADPFAFSQTLLPAHFKHSNFSSFVRQLN 71

Query: 72  TYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLR--EMG------------- 131
           TYGFRKVDPDRWEFA+  FLR Q HLLR IVRR      + R  E G             
Sbjct: 72  TYGFRKVDPDRWEFAHVSFLRGQTHLLRRIVRRSSGGGGAKRKEEAGGCGGGGEAAAGDV 131

Query: 132 DEE---LVTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMM 191
           DEE   +  E+ARLR+EQ+ +E  +  M +R++ TE+RP+QM+AFL KV  DP++L R++
Sbjct: 132 DEESAVVALEVARLRREQREIEGRVAAMWRRVQETERRPKQMLAFLVKVVGDPQVLRRLV 191

Query: 192 ---------LQKDRPGPHIGDKKRRLMISSSSSS 199
                       D    H   K+ RL++ SSS++
Sbjct: 192 DRDNTNAAASNADDSAVHHQVKRPRLLLDSSSTT 225

BLAST of Cp4.1LG04g09910.1 vs. ExPASy Swiss-Prot
Match: Q942D6 (Heat stress transcription factor C-1b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFC1B PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.1e-44
Identity = 98/183 (53.55%), Postives = 126/183 (68.85%), Query Frame = 0

Query: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60
           + A  +     VAPFV KT+ MV+DP T+  + WG A N+FLVLDP  FS  LLP+YFKH
Sbjct: 11  LQAAGDGGPGAVAPFVAKTFHMVSDPSTNAVVRWGGAGNTFLVLDPAAFSDFLLPSYFKH 70

Query: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSS-----LREM 120
            NF+SFVRQLNTYGFRKVDPDRWEFA++ FLR Q  LL  IVR+K+   ++     L E 
Sbjct: 71  RNFASFVRQLNTYGFRKVDPDRWEFAHESFLRGQAQLLPRIVRKKKKGGAAPGCRELCEE 130

Query: 121 GDE--ELVTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMM 177
           G+E    +  + RLR+EQ+ +EEEL+ M +RL   E RP QMMAFL K+A++P ++ R M
Sbjct: 131 GEEVRGTIEAVQRLREEQRGMEEELQAMDQRLRAAESRPGQMMAFLAKLADEPGVVLRAM 190

BLAST of Cp4.1LG04g09910.1 vs. ExPASy Swiss-Prot
Match: Q0DBL6 (Heat stress transcription factor C-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFC2B PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 7.2e-44
Identity = 98/180 (54.44%), Postives = 123/180 (68.33%), Query Frame = 0

Query: 13  APFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFSSFVRQLNT 72
           APFV KTY+MV DP TD  I WG  NNSF+V DP  FS TLLPA+FKHNNFSSFVRQLNT
Sbjct: 10  APFVWKTYRMVEDPGTDGVIGWGKGNNSFVVADPFVFSQTLLPAHFKHNNFSSFVRQLNT 69

Query: 73  YGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQST--------NSSLREM--------G 132
           YGFRKVDPDRWEFA+  FLR Q HLLRNIVRR  +             R+         G
Sbjct: 70  YGFRKVDPDRWEFAHASFLRGQTHLLRNIVRRGSAAAGGGGGGGGGKRRDASADGGGGGG 129

Query: 133 DEEL---VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMM 174
           DE++    TE+ RL++EQ+ +++ +  M +R++ TE+RP+QM+AFL KV  D + L R++
Sbjct: 130 DEDMTMVATEVVRLKQEQRTIDDRVAAMWRRVQETERRPKQMLAFLLKVVGDRDKLHRLV 189

BLAST of Cp4.1LG04g09910.1 vs. ExPASy Swiss-Prot
Match: Q40152 (Heat shock factor protein HSF8 OS=Solanum lycopersicum OX=4081 GN=HSF8 PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.3e-41
Identity = 101/230 (43.91%), Postives = 131/230 (56.96%), Query Frame = 0

Query: 14  PFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFSSFVRQLNTY 73
           PF++KTY MV+DP TD  ++W   NNSF+V DP  F+  LLP YFKHNNFSSFVRQLNTY
Sbjct: 39  PFLVKTYDMVDDPSTDKIVSWSPTNNSFVVWDPPEFAKDLLPKYFKHNNFSSFVRQLNTY 98

Query: 74  GFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLRE------------------- 133
           GFRKVDPDRWEFAN+ FLR Q HLL++I RRK +   + ++                   
Sbjct: 99  GFRKVDPDRWEFANEGFLRGQKHLLKSISRRKPAHGHAQQQQQPHGNAQQQMQPPGHSAS 158

Query: 134 ---------MGDEE-----------LVTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQ 193
                     G EE           L+ E+ RLR++Q+  + +L+GM +RL+  E R QQ
Sbjct: 159 VGACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQATDNQLQGMVQRLQGMELRQQQ 218

Query: 194 MMAFLHKVAEDPEILPRMMLQKDRPGPHI--GDKKRRLMISSSSSSPSTT 203
           MM+FL K    P  L + + Q++     I  G KKRR+     S  PS T
Sbjct: 219 MMSFLAKAVNRPGFLAQFVQQQNESNKRIAEGSKKRRIKQDIESQDPSVT 268

BLAST of Cp4.1LG04g09910.1 vs. NCBI nr
Match: XP_023530778.1 (heat stress transcription factor C-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578 bits (1490), Expect = 5.27e-208
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0

Query: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60
           MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH
Sbjct: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120
           NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL
Sbjct: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120

Query: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180
           VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG
Sbjct: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180

Query: 181 PHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ 240
           PHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ
Sbjct: 181 PHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ 240

Query: 241 RWNLAEAEAGGMDISRSRGGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291
           RWNLAEAEAGGMDISRSRGGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF
Sbjct: 241 RWNLAEAEAGGMDISRSRGGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291

BLAST of Cp4.1LG04g09910.1 vs. NCBI nr
Match: KAG6587956.1 (Heat stress transcription factor C-1b, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 569 bits (1466), Expect = 2.40e-204
Identity = 287/291 (98.63%), Postives = 288/291 (98.97%), Query Frame = 0

Query: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60
           MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH
Sbjct: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120
           NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL
Sbjct: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120

Query: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180
           VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG
Sbjct: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180

Query: 181 PHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ 240
           PHIGDKKRRLMISSSSSSPSTTTVTTTS+EAEEIEEEGSWRPNFSS EVSGPAGFCNHVQ
Sbjct: 181 PHIGDKKRRLMISSSSSSPSTTTVTTTSMEAEEIEEEGSWRPNFSSLEVSGPAGFCNHVQ 240

Query: 241 RWNLAEAEAGGMDISRSRGGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291
           RWNLAEAEAGGMDISRSR GGE KNSTEMMGTVEESNPAPPYPFSLFGCGF
Sbjct: 241 RWNLAEAEAGGMDISRSRSGGEKKNSTEMMGTVEESNPAPPYPFSLFGCGF 291

BLAST of Cp4.1LG04g09910.1 vs. NCBI nr
Match: KAG7021846.1 (Heat stress transcription factor C-1b, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 568 bits (1463), Expect = 6.88e-204
Identity = 286/291 (98.28%), Postives = 288/291 (98.97%), Query Frame = 0

Query: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60
           MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH
Sbjct: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120
           NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNS+LREMGDEEL
Sbjct: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSNLREMGDEEL 120

Query: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180
           VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG
Sbjct: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180

Query: 181 PHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ 240
           PHIGDKKRRLMISSSSSSPSTTTVTTTS+EAEEIEEEGSWRPNFSS EVSGPAGFCNHVQ
Sbjct: 181 PHIGDKKRRLMISSSSSSPSTTTVTTTSMEAEEIEEEGSWRPNFSSLEVSGPAGFCNHVQ 240

Query: 241 RWNLAEAEAGGMDISRSRGGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291
           RWNLAEAEAGGMDISRSR GGE KNSTEMMGTVEESNPAPPYPFSLFGCGF
Sbjct: 241 RWNLAEAEAGGMDISRSRSGGEKKNSTEMMGTVEESNPAPPYPFSLFGCGF 291

BLAST of Cp4.1LG04g09910.1 vs. NCBI nr
Match: XP_022933445.1 (heat stress transcription factor C-1-like [Cucurbita moschata])

HSP 1 Score: 566 bits (1460), Expect = 1.97e-203
Identity = 287/291 (98.63%), Postives = 287/291 (98.63%), Query Frame = 0

Query: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60
           MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH
Sbjct: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120
           NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL
Sbjct: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120

Query: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180
           VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG
Sbjct: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180

Query: 181 PHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ 240
            HIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWR NFSSPEVSGPAGFCNHVQ
Sbjct: 181 AHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRRNFSSPEVSGPAGFCNHVQ 240

Query: 241 RWNLAEAEAGGMDISRSRGGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291
            WNLAEAEAGGMDISRSR GGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF
Sbjct: 241 GWNLAEAEAGGMDISRSRSGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291

BLAST of Cp4.1LG04g09910.1 vs. NCBI nr
Match: XP_022973632.1 (heat stress transcription factor C-1-like [Cucurbita maxima])

HSP 1 Score: 562 bits (1449), Expect = 9.37e-202
Identity = 284/291 (97.59%), Postives = 286/291 (98.28%), Query Frame = 0

Query: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60
           MDAN+ESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH
Sbjct: 1   MDANSESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120
           NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNS L EMGDEEL
Sbjct: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSRLCEMGDEEL 120

Query: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180
           VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG
Sbjct: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180

Query: 181 PHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ 240
            HIGDKKRRLMISSSSSSPSTTT+TTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ
Sbjct: 181 AHIGDKKRRLMISSSSSSPSTTTMTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ 240

Query: 241 RWNLAEAEAGGMDISRSRGGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291
            WNLAEAEAGGMDISRSR GGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF
Sbjct: 241 AWNLAEAEAGGMDISRSRSGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291

BLAST of Cp4.1LG04g09910.1 vs. ExPASy TrEMBL
Match: A0A6J1F4W8 (heat stress transcription factor C-1-like OS=Cucurbita moschata OX=3662 GN=LOC111440859 PE=3 SV=1)

HSP 1 Score: 566 bits (1460), Expect = 9.55e-204
Identity = 287/291 (98.63%), Postives = 287/291 (98.63%), Query Frame = 0

Query: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60
           MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH
Sbjct: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120
           NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL
Sbjct: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120

Query: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180
           VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG
Sbjct: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180

Query: 181 PHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ 240
            HIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWR NFSSPEVSGPAGFCNHVQ
Sbjct: 181 AHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRRNFSSPEVSGPAGFCNHVQ 240

Query: 241 RWNLAEAEAGGMDISRSRGGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291
            WNLAEAEAGGMDISRSR GGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF
Sbjct: 241 GWNLAEAEAGGMDISRSRSGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291

BLAST of Cp4.1LG04g09910.1 vs. ExPASy TrEMBL
Match: A0A6J1I819 (heat stress transcription factor C-1-like OS=Cucurbita maxima OX=3661 GN=LOC111472225 PE=3 SV=1)

HSP 1 Score: 562 bits (1449), Expect = 4.54e-202
Identity = 284/291 (97.59%), Postives = 286/291 (98.28%), Query Frame = 0

Query: 1   MDANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60
           MDAN+ESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH
Sbjct: 1   MDANSESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEEL 120
           NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNS L EMGDEEL
Sbjct: 61  NNFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSRLCEMGDEEL 120

Query: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180
           VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG
Sbjct: 121 VTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPG 180

Query: 181 PHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ 240
            HIGDKKRRLMISSSSSSPSTTT+TTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ
Sbjct: 181 AHIGDKKRRLMISSSSSSPSTTTMTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQ 240

Query: 241 RWNLAEAEAGGMDISRSRGGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291
            WNLAEAEAGGMDISRSR GGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF
Sbjct: 241 AWNLAEAEAGGMDISRSRSGGEMKNSTEMMGTVEESNPAPPYPFSLFGCGF 291

BLAST of Cp4.1LG04g09910.1 vs. ExPASy TrEMBL
Match: A0A1S3CJ61 (heat stress transcription factor C-1-like OS=Cucumis melo OX=3656 GN=LOC103501554 PE=3 SV=1)

HSP 1 Score: 388 bits (997), Expect = 3.11e-133
Identity = 217/303 (71.62%), Postives = 238/303 (78.55%), Query Frame = 0

Query: 5   NESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFS 64
           N S  DIVAPFVLKTYQMVNDPLTD+FI+WGSANNSF+VLDPLHFSHTLLPA+FKHNNFS
Sbjct: 4   NSSNKDIVAPFVLKTYQMVNDPLTDSFISWGSANNSFIVLDPLHFSHTLLPAFFKHNNFS 63

Query: 65  SFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSL--REMGDEELVT 124
           SFVRQLNTYGFRKVDPDRWEFAN+WFLR QF LL+NIVRRKQS N  L   +MGDEELVT
Sbjct: 64  SFVRQLNTYGFRKVDPDRWEFANEWFLRGQFQLLKNIVRRKQSCNKYLIGDQMGDEELVT 123

Query: 125 EIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPGPH 184
           EIARLRKEQ+ LEE+LEGMKKRLETTEKRPQQMMAFLHKV EDPEILPR+ML KD     
Sbjct: 124 EIARLRKEQRALEEQLEGMKKRLETTEKRPQQMMAFLHKVVEDPEILPRIMLHKDPTRLQ 183

Query: 185 IGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGS----WRPNFSSPEVSGPAGFCNH 244
           + +KKRRLMISSSSSSPSTTT+T    E +EIEE+ +    + PNFSSPEVSGP      
Sbjct: 184 LSEKKRRLMISSSSSSPSTTTLT----EGDEIEEDSNNPQDFGPNFSSPEVSGP------ 243

Query: 245 VQRWNLAEAEAG---------GMDISRSRGGGEMKNSTEMMGTVEESNPAPP-YPFSLFG 291
              W L EAE            +D+  S  GGE K  TEM  TVEE++P PP YPFSLFG
Sbjct: 244 ---WFLREAEGNLGIPIPPVPAIDLGISSNGGERKYLTEM--TVEETHPPPPPYPFSLFG 291

BLAST of Cp4.1LG04g09910.1 vs. ExPASy TrEMBL
Match: A0A0A0LWD5 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G240590 PE=3 SV=1)

HSP 1 Score: 381 bits (978), Expect = 2.41e-130
Identity = 214/303 (70.63%), Postives = 232/303 (76.57%), Query Frame = 0

Query: 5   NESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFS 64
           N   NDIVAPFVLKTYQMVNDPLTD FI+WGSANNSF+VLDPLHFSHTLLPA+FKHNNFS
Sbjct: 4   NSGNNDIVAPFVLKTYQMVNDPLTDPFISWGSANNSFIVLDPLHFSHTLLPAFFKHNNFS 63

Query: 65  SFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSL--REMGDEELVT 124
           SFVRQLNTYGFRKVDPDRWEFAN+WFLR QF LL+NIVRRKQS N  L   +MGDEELVT
Sbjct: 64  SFVRQLNTYGFRKVDPDRWEFANEWFLRGQFQLLKNIVRRKQSCNKYLVGDQMGDEELVT 123

Query: 125 EIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPGPH 184
           EI RLRKEQ+ LEE+LEGMKKRLETTEKRPQQMMAFLHKV EDPEILPR+ML KD     
Sbjct: 124 EIGRLRKEQRALEEQLEGMKKRLETTEKRPQQMMAFLHKVVEDPEILPRIMLHKDPTRLQ 183

Query: 185 IGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGS----WRPNFSSPEVSGPAGFCNH 244
             +KKRRLMISSSSSSPSTTT+T    E +EIEE+ +    + PN SSPEV GP      
Sbjct: 184 FAEKKRRLMISSSSSSPSTTTLT----EGDEIEEDSNNQQDFGPNLSSPEVCGP------ 243

Query: 245 VQRWNLAEAEAG---------GMDISRSRGGGEMKNSTEMMGTVEESNPAPP-YPFSLFG 291
              W + EAE            +DI  S  GGE K  TEM  TVEE+ P PP YPFSLFG
Sbjct: 244 ---WFIGEAEGNLGIPIPTVQAIDIGISSNGGERKYLTEM--TVEETYPPPPPYPFSLFG 291

BLAST of Cp4.1LG04g09910.1 vs. ExPASy TrEMBL
Match: A0A6J1H8H5 (heat stress transcription factor C-1-like OS=Cucurbita moschata OX=3662 GN=LOC111461514 PE=3 SV=1)

HSP 1 Score: 355 bits (911), Expect = 2.79e-120
Identity = 198/297 (66.67%), Postives = 223/297 (75.08%), Query Frame = 0

Query: 9   NDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFSSFVR 68
           NDIVAPF+LKTYQMVNDPLTDT ITWGSANNSF+VLDPLHFSHTLLPA+FKHNNFSSF+R
Sbjct: 7   NDIVAPFILKTYQMVNDPLTDTSITWGSANNSFVVLDPLHFSHTLLPAFFKHNNFSSFIR 66

Query: 69  QLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLR------EMGDEELVT 128
           QLNTYGFRKVDPDRWEFAN+WFLR Q+HLLRNIVRRK  +NS+++      +M DE+LV+
Sbjct: 67  QLNTYGFRKVDPDRWEFANEWFLRGQYHLLRNIVRRKHCSNSAVKLVGVGGDMSDEDLVS 126

Query: 129 EIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDRPGP- 188
           EI RLRKEQ+ LE +L+ MKKRLETTEKRP QMMAFLHKV EDPEILPR+ML +D     
Sbjct: 127 EIGRLRKEQRALEHQLQAMKKRLETTEKRPHQMMAFLHKVVEDPEILPRIMLHRDPTHRF 186

Query: 189 HIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEEGSWRPNFSSPEVSGPAGFCNHVQR 248
            I DKKRR M S SSSSPST      S + +EIE++ +       PEVSGP         
Sbjct: 187 QIPDKKRRFMTSPSSSSPSTA-----SFDTDEIEDDPT------RPEVSGP--------- 246

Query: 249 WNLAEAEAGG------MDISRSRGGGEMKNSTEM-MGTVEESNPAPPYPFSLFGCGF 291
           W L +  A G      MDI  S  GGEMK  TEM +G VEES P PPYPFSLFGCGF
Sbjct: 247 WFLEDPAAFGVPAVAPMDIGISSSGGEMKYLTEMTVGAVEESRPPPPYPFSLFGCGF 283

BLAST of Cp4.1LG04g09910.1 vs. TAIR 10
Match: AT3G24520.1 (heat shock transcription factor C1 )

HSP 1 Score: 258.8 bits (660), Expect = 5.1e-69
Identity = 157/337 (46.59%), Postives = 200/337 (59.35%), Query Frame = 0

Query: 2   DANNESANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHN 61
           ++NN + N+++APF++KTYQMVNDP TD  ITWG A+NSF+V+DPL FS  +LPAYFKHN
Sbjct: 5   NSNNNNNNNVIAPFIVKTYQMVNDPSTDWLITWGPAHNSFIVVDPLDFSQRILPAYFKHN 64

Query: 62  NFSSFVRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRKQSTNSSLREMGDEELV 121
           NFSSFVRQLNTYGFRKVDPDRWEFAN+ FLR Q HLL NI RRK +     +++ D E+V
Sbjct: 65  NFSSFVRQLNTYGFRKVDPDRWEFANEHFLRGQKHLLNNIARRKHARGMYGQDLEDGEIV 124

Query: 122 TEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILPRMMLQKDR--P 181
            EI RL++EQ+ LE E++ M +R+E TEKRP+QMMAFL+KV EDP++LPRMML+K+R   
Sbjct: 125 REIERLKEEQRELEAEIQRMNRRIEATEKRPEQMMAFLYKVVEDPDLLPRMMLEKERTKQ 184

Query: 182 GPHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIEEE--------GSWRPNFSSPE--- 241
              + DKK+R             T++T   E EE+EE+         S  P+ SS E   
Sbjct: 185 QQQVSDKKKR-----------RVTMSTVKSEEEEVEEDEGRVFRVMSSSTPSPSSTENLY 244

Query: 242 -VSGPAGFCNHVQRWNLAEAEAG----------------------GMDISRSRGGG---- 292
               P G+   + +      E G                       +  S + GGG    
Sbjct: 245 RNHSPDGWIVPMTQGQFGSYETGLVAKSMLSNSTSSTSSSLTSTFSLPESVNGGGGGGCG 304

BLAST of Cp4.1LG04g09910.1 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 169.1 bits (427), Expect = 5.3e-42
Identity = 106/246 (43.09%), Postives = 146/246 (59.35%), Query Frame = 0

Query: 7   SANDIVAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFSSF 66
           +AN +  PF+ KTY MV DP TD  ++W   NNSF+V DP  FS  LLP YFKHNNFSSF
Sbjct: 45  NANSLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSF 104

Query: 67  VRQLNTYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRK-------QSTN---------- 126
           VRQLNTYGFRKVDPDRWEFAN+ FLR Q HLL+ I RRK        S+N          
Sbjct: 105 VRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQ 164

Query: 127 ------SSLREMG----DEE----------LVTEIARLRKEQKVLEEELEGMKKRLETTE 186
                 SS  E+G    +EE          L+ E+ +LR++Q+  + +L+ + K L+  E
Sbjct: 165 GSMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVME 224

Query: 187 KRPQQMMAFLHKVAEDPEILPRMMLQKDRPGPHI--GDKKRRLMISSSSSSPSTTTVTTT 214
           +R QQ+M+FL K  ++P  L + + ++     H+   +KKRRL   S++++ S +   + 
Sbjct: 225 QRQQQIMSFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRLREDSTAATESNS--HSH 284

BLAST of Cp4.1LG04g09910.1 vs. TAIR 10
Match: AT3G02990.1 (heat shock transcription factor A1E )

HSP 1 Score: 167.2 bits (422), Expect = 2.0e-41
Identity = 98/218 (44.95%), Postives = 132/218 (60.55%), Query Frame = 0

Query: 12  VAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFSSFVRQLN 71
           + PF+ KTY MV+DPLTD  ++W S NNSF+V +   F+   LP YFKHNNFSSFVRQLN
Sbjct: 21  IPPFLSKTYDMVDDPLTDDVVSWSSGNNSFVVWNVPEFAKQFLPKYFKHNNFSSFVRQLN 80

Query: 72  TYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRK-------------QSTNSSLREMG-- 131
           TYGFRKVDPDRWEFAN+ FLR Q  +L++IVRRK              S+  +  E+G  
Sbjct: 81  TYGFRKVDPDRWEFANEGFLRGQKQILKSIVRRKPAQVQPPQQPQVQHSSVGACVEVGKF 140

Query: 132 --DEE----------LVTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAE 191
             +EE          L+ E+ RLR++Q+V E  L+ + +++   E+R QQMM+FL K  +
Sbjct: 141 GLEEEVERLQRDKNVLMQELVRLRQQQQVTEHHLQNVGQKVHVMEQRQQQMMSFLAKAVQ 200

Query: 192 DPEILPRMMLQKDRPGPHIGD--KKRRLMISSSSSSPS 201
            P  L +   Q +    HI +  KKRRL +    +S S
Sbjct: 201 SPGFLNQFSQQSNEANQHISESNKKRRLPVEDQMNSGS 238

BLAST of Cp4.1LG04g09910.1 vs. TAIR 10
Match: AT2G26150.1 (heat shock transcription factor A2 )

HSP 1 Score: 164.5 bits (415), Expect = 1.3e-40
Identity = 103/229 (44.98%), Postives = 137/229 (59.83%), Query Frame = 0

Query: 14  PFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFSSFVRQLNTY 73
           PF+ KTY+MV DP TDT ++W +  NSF+V D   FS TLLP YFKH+NFSSF+RQLNTY
Sbjct: 44  PFLTKTYEMVEDPATDTVVSWSNGRNSFVVWDSHKFSTTLLPRYFKHSNFSSFIRQLNTY 103

Query: 74  GFRKVDPDRWEFANQWFLRDQFHLLRNIVRRK---------QSTNSSLREMG----DEE- 133
           GFRK+DPDRWEFAN+ FL  Q HLL+NI RR+         Q +  S  E+G    D E 
Sbjct: 104 GFRKIDPDRWEFANEGFLAGQKHLLKNIKRRRNMGLQNVNQQGSGMSCVEVGQYGFDGEV 163

Query: 134 ---------LVTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVAEDPEILP 193
                    LV E+ RLR++Q   + ++  M++RL  TEKR QQMM FL K   +P  + 
Sbjct: 164 ERLKRDHGVLVAEVVRLRQQQHSSKSQVAAMEQRLLVTEKRQQQMMTFLAKALNNPNFVQ 223

Query: 194 RMML----QKDRPGPHIGDKKRRLMISSSSSSPSTTTVTTTSLEAEEIE 216
           +  +    +K   G  +G +KRRL     +S+PS  T+    L  +E +
Sbjct: 224 QFAVMSKEKKSLFGLDVG-RKRRL-----TSTPSLGTMEENLLHDQEFD 266

BLAST of Cp4.1LG04g09910.1 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 163.7 bits (413), Expect = 2.2e-40
Identity = 96/217 (44.24%), Postives = 130/217 (59.91%), Query Frame = 0

Query: 12  VAPFVLKTYQMVNDPLTDTFITWGSANNSFLVLDPLHFSHTLLPAYFKHNNFSSFVRQLN 71
           V PF+ KTY MV+DPLT+  ++W S NNSF+V     FS  LLP YFKHNNFSSFVRQLN
Sbjct: 25  VPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLN 84

Query: 72  TYGFRKVDPDRWEFANQWFLRDQFHLLRNIVRRK--------QSTNSSLREMG------- 131
           TYGFRKVDPDRWEFAN+ FLR +  LL++IVRRK        Q T      +G       
Sbjct: 85  TYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEVGK 144

Query: 132 ---DEE----------LVTEIARLRKEQKVLEEELEGMKKRLETTEKRPQQMMAFLHKVA 191
              +EE          L+ E+ RLR++Q+  E +L+ + ++++  E+R QQMM+FL K  
Sbjct: 145 FGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAV 204

Query: 192 EDPEILPRMMLQKDRPGPHI---GDKKRRLMISSSSS 198
           + P  L +++ Q +  G       +KKRRL +    +
Sbjct: 205 QSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQEN 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LV527.2e-6846.59Heat stress transcription factor C-1 OS=Arabidopsis thaliana OX=3702 GN=HSFC1 PE... [more]
Q6EUG41.0e-4550.93Heat stress transcription factor C-2a OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q942D61.1e-4453.55Heat stress transcription factor C-1b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q0DBL67.2e-4454.44Heat stress transcription factor C-2b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q401523.3e-4143.91Heat shock factor protein HSF8 OS=Solanum lycopersicum OX=4081 GN=HSF8 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023530778.15.27e-208100.00heat stress transcription factor C-1-like [Cucurbita pepo subsp. pepo][more]
KAG6587956.12.40e-20498.63Heat stress transcription factor C-1b, partial [Cucurbita argyrosperma subsp. so... [more]
KAG7021846.16.88e-20498.28Heat stress transcription factor C-1b, partial [Cucurbita argyrosperma subsp. ar... [more]
XP_022933445.11.97e-20398.63heat stress transcription factor C-1-like [Cucurbita moschata][more]
XP_022973632.19.37e-20297.59heat stress transcription factor C-1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1F4W89.55e-20498.63heat stress transcription factor C-1-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1I8194.54e-20297.59heat stress transcription factor C-1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A1S3CJ613.11e-13371.62heat stress transcription factor C-1-like OS=Cucumis melo OX=3656 GN=LOC10350155... [more]
A0A0A0LWD52.41e-13070.63HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G240590 ... [more]
A0A6J1H8H52.79e-12066.67heat stress transcription factor C-1-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G24520.15.1e-6946.59heat shock transcription factor C1 [more]
AT4G17750.15.3e-4243.09heat shock factor 1 [more]
AT3G02990.12.0e-4144.95heat shock transcription factor A1E [more]
AT2G26150.11.3e-4044.98heat shock transcription factor A2 [more]
AT5G16820.12.2e-4044.24heat shock factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 117..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 190..209
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 176..223
NoneNo IPR availablePANTHERPTHR10015:SF332HEAT STRESS TRANSCRIPTION FACTOR C-1coord: 13..285
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 53..65
score: 78.24
coord: 15..38
score: 38.69
coord: 66..78
score: 87.03
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 11..104
e-value: 4.3E-47
score: 172.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 15..104
e-value: 3.4E-28
score: 98.1
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 54..78
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 7..105
e-value: 4.1E-33
score: 115.9
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 13..285
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 12..104

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG04g09910Cp4.1LG04g09910gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG04g09910.1:three_prime_utr:001Cp4.1LG04g09910.1:three_prime_utr:001three_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG04g09910.1:exon:002Cp4.1LG04g09910.1:exon:002exon
Cp4.1LG04g09910.1:exon:001Cp4.1LG04g09910.1:exon:001exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG04g09910.1:cds:002Cp4.1LG04g09910.1:cds:002CDS
Cp4.1LG04g09910.1:cds:001Cp4.1LG04g09910.1:cds:001CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG04g09910.1:five_prime_utr:001Cp4.1LG04g09910.1:five_prime_utr:001five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG04g09910.1Cp4.1LG04g09910.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0034605 cellular response to heat
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding