Cp4.1LG04g09200.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG04g09200.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	calmodulin-binding receptor-like cytoplasmic kinase 3
Location	Cp4.1LG04: 10222366 .. 10227609 (+)
Sequence length	2753
RNA-Seq Expression	Cp4.1LG04g09200.1
Synteny	Cp4.1LG04g09200.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGATAAAATTCCAACTATCTGATCGACTCCGCTGCAGCCGCGTCAAGATGAAGAACAATCCACATAATCATGAAAACAACTACTGGAGTCCAGTCAACTTCGATCCTGGCCGTTGATTTGAAATGGATGTAACATTTTTTGTATTTATTAATCATCTGGAAAATATCACAGGAGTTTGACGAAGCGACGAAATTTATGTTCAAATCAACTACTGGAGCAAAGGATTGTTGAGGAAATGGTGTTTTCCGAGCGGTCAACTTTATCCAAGCCTATCGCTTCCAACTCCATTTATCTATGGTACATCGCCTTCTCTCTCGACGATTCTTTGTTGTTGCTATTGGTTGTTTTTCACTCTATTGTTAGCTTCGCCGCCGATGGGATTCTTCCTGCTGTTATTCTGATCGGAGCTTCATTTGGTTGGCCTCTGAGTGTTGGCGATTCGTGAAGTGAGTTTAGATCGTCTCTCCGAGTTCCGGTAAGTTTTGGTGGTGCTGTTGAACTAGGGCAATTTGGGAGTGGCTTTGGAAAATTGTTGCTATTCGTCTCAGCGGTTATGATTGGGATGGAATAAATTGATTAAATAGTTTTGATCGCTTTCTTAGGGTTTGATTTCCAAAGATGGCTGTTCTTCTTGTTATGTCATCGATAATAGCGTCCGTCATGGGCGTGTTGCTGGCCCTATCGCCAAGAGTATATGCTCATGAGTTTATGATGGAATCAGGGGTTTCAAGTGCTCATCAGATTCCTTACTCGGTTTCGGATGGAATCAGGTGTTTCAAATGCAACTTTTTTGCAGATGATATTTCAGTTAAAACATCAAGGAAACTGTTAAGGGAAGAAAGGGAGGAGGAATACACTAAGCTTAAAGATGGTGATTATCCTCATCATAGAGCGAAACAGGATGGGAAATCTTTGGCAACCTCCCAAACTATTGGAATGGTAGTGACTGGAACATTCGTTTTATGTTTTGGTGTTCTCTGTCCTTGTTTTTATCGAAAGAGGAGACGCACAGTTCAAAGAGGCATTGAAATAGAACAACCATCAGGTGAGTCATTGGTTCTATATGAATTTCTTGCAGTTTATGAGGATAGGAGTATTGTTAGTTAAAGATTCTACCTCACCTTTGTGAGATTCCACGTTGGTTGGAGAGGAGAACAAACATTCCTCATAAGGGTGTGGAAACCTCTCCCTAGTATATGCGTTTAGAAACTTTGAGGGGAAACCCAGAAGGAAAAGTCTAAAAAGGACAATATTTGCTAGCGGTGTGTTTGGGCTGTTACAAAGGGTATTAGAGCTAGACACTGGGAGGTGTGCTAGTGATGATGCTGGGCCCTATGGGGTGGATTGTGAGATCCCACATCGGTGGGAGAGGGGAACGAAACATTTCTTACAAGAGTGTGAAAACCTCTCCCTAGCATACGCGCTTTTTAAATTTTGAGAGGAAAGCCTAAAAAGGAGAATATCTGGTAGCGGTGGGCTTGAGCTGTTACAAATGGTATTAGAGCCAGACATTAGGAGGTGTGCCAGCGATGACGCTGGCCCCCAAGAAGGGTGGATTGTGAGATCCCACAAAGAATTCCTTATAAGAGTGTAGAAACCTCTCCCTAACATACACATTTTGAAATTTTGAGGGGAAACTTAGAAGGACAAGTTCAAAGAGGACAATATCTTCTAGCGGTGGGCTTGGGCGGTTACAAATAGTATCAGAGCCTAACACTGGGAGGTGTACTAGCAAGGACGTTGGGCCCTCCAAGGGGGGTGGATTGTGAGATCCCACATTGGTTGGAGACGGGAACGAAGTATTCCTTATAAGGGCGTGGAAACAGCCCTAGCATACACGTGGGAAACCCAGAAGAAAAAGCCCAAAGAGGACCAAATGAGCTTGAAAGCCATTAAAAGTGGCAACTCAATTTAGAATTTTTGTTATCCATTAAGAAGGGCCATTGCAAGATGAACATTTTTATTCACTCTTCAGTCACTCATTTTTCTTCTCAATATGTCAATGAATGCAGTATGTCTAAATTGTGTATTTTGTGTCCTTTTTTGACAAAGGCACTGCCCTTCCTTGACATTACCTGGTGAGCATCAAATGTTCTCGAAATTGAGTTATAAATTCTTTCTTTCAGTTCATTCAGCCTCCATTGAAGTGATGAACCCTGCTCCTGAAAAGGTTCCGGCCAGTCCACTTCGAGTACCACCTAGTCCTAGATATTCCCCATCTCCCAGATTCAAACGCCTTGGATCTGTACATCTCAACATGAGCCAAGTAGCCAGAGCAACTCAAGATTTCTCCCCAGCTCTGCGCATAGGCGAAGGAGGTTTTGGAACTGTCTACAAAGCACAACTAGAAAATGGGCATGTAGTTGCCATAAAACGTTCAAAAAAGGCAAGTACCAAATTCTTATGCTGTTTTTATGATGATAGGATATGTAAAATCTCAAACCCTTGTTTCTGAGAATAGTAACAGCATCTCTAACACAATTATTTTGCAGGAACTGTTTGAGAATTCACGAACTGACTTTAGCAGTGAAGTAGAGTTACTGTCCAAAATTGATCATCGGAGTCTGGTTAAGCTGCTAGGTCATGTTGATCATGGAAATGAGCGCATTATCATTACGGAATATGTAGGAAATGGTACTCTTAGAGAACACTTGGACGGTAAGGTTTCTACCCTATGGAAGACCAAAAATTAACCTGTTGTTTAACTCTGATGGCTTCTTTAAGGCTCATGACTGCGCTTTTATCATTGTAGGCGTGCATGGAAAAGTATTGGATTTTAATCAGAGACTTGAAATTGCCATTGACATTGCTCATGGACTAACTTACCTCCATATGTATGCAGGTTAGAATCTTTTTCATTAGTCGGCTTATAAATGTCACTCATTTTTTTGATATCAGGAAGGGCCAACTTTGAAAGTGGACATAATTACTAACGCAACACATCCATTTGATTGTGTGTATATTTTAACATATATAGAAGAGTTTGTTTAATTAGCAATTATTTTATGTCTCAAATAATATTTATTTTTAAAAAATTATTTTAAGAGAAGATTTTTGTGGATCTGCAATTACTGCTCAAAGTCCCTCGGTAAGAATATTATGAACACCGACTTGCACACGCCTCAATTATCTGTAATGATCTAAGCCTATCGCTAGCAGATATTGTCCTCTTTGAGCTTTTCCTTTCGGACTTTCCTTAAGGTTTTAAAACGCGTCTGTAGGAAGAGGCTTCCACACCCTTATAAACAATGTTCGTTTTCCTCCCTAACCAATGTCAGATCTCACAATCCACTCCCCTTCAAGGTCTAGCGTTCTCGCTGATACTCGTTCCCTTCTTTAATCGATGTGAGACCCCCCAATCCACCACTTTCGGGACCTAGCGTCCTTGCTGGCACACCGCCTCGTGTCCACCCCCCTTCGAGACTCAGCCTCCTCGTTGGCATGTCGCTCGGTGTCTAGCTCTGGTACCATTCATAATGACTCAAGTCCACAGCTAGCAGATATTGTCCTCTTTGGACTTTTTCTTCCGGACTAACTCTCAAGGTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCCACACCCTTATAAAGAGATGCGAGATCTCACACTATCTCTCATGACAACCACCTGACCCTACCATAGCTGGTCGACAACAGAACTTTTAGGACATTAAATCCTTGTTAGGCGGCTACCATGGCTTGAAAACTTTTATTATTACCTTATTGACCACTAGTCCCAAGCTTTGTTTAGGGTTATCTTATTGGATATTTTCTGCTGTCCAGCCAGTTAACCTTTTTTTTTCTTGTTGACAATAGAGAAGCAAATTATTCACAGAGATGTTAAGTCTACCAACATTCTTCTTACCGAAAACATGAGAGCAAAGGTGGCGGATTTTGGATTTGCAAGGCTTGGAACATTGAGTACAGATCAAACGCACATTTCAACTCAGGTGAAAGGAACTGTTGGTTACCTTGACCCAGAGTATATGAAGACTTATCAACTTACTACCAAGAGTGATGTCTACTCATTTGGGATTTTACTCGTAGAAATTCTAACAGGACGCCGTCCATTGGAAGTTAAGAGGCCTCCTGATGAGCGGGTCACAATAAGATGGGTGAGTTAAACATTCTAATCATTTTCTTAAGAAACCTTTCAAGTTTCGAACGAACTTTTTACTCATCGTTTCTGAAAGATCGCTTCTTGTTTTACTTTGATTCGTTGCAGGCTTTCAACAAGTATAGTGAAAACAAAATTCTGGAAACCTTAGATCCTTCAATGGAGGAAACTGTTGACGCAGAGATTGTCATCAAAATGTTCAAATTGGCAATCCAATGTGCAGCACCGGTACGAGCCGAACGACCAGACATGAAATTTGTTGGGGAGCAGCTGTGGTCAATAAGAGCAAACTATATGTCACAGAAGAAGGCAAGATTACCTTAAAAAGGTGTGTTGTGTTGGAAACTCCAGAGGTGACCCACTGTGATTTCATCGATTTACCATGTCATTTAGAAGTTCACCTCCCTTCTTCAGAGTCTTTCCACGTTTGATGCTTTACTTAATTACCCTGTCATTAGAGTTTTCACCTCCAACAGATATCATTCATCACTGTTTTAATAGGTGAATCACCTATTAATTTGGAGTTTTTCTTTTCCTTCAACTTCTCTAGAATTGAAGTCAACAACACTTGTGTACCATTGTAAAAAAGGGGGGTTATTCCCAATTTGATGGCAAATGATATTAAAAATTGATATTGCAATTGAAATGGTAAATAATGACAATAATTTTAATTTTGTATATAGTTGAAATTGTGTCGGAGATATTGTCACCATGTCTTCTTTCAATTTTTACCTTATTGCCATGTTAACTTCCATTCATCATTTCAATTGAGAAATATTGGGAAGTTTCCTTGGGATTACTAAAATATATTAATAGCCACCGGGCATGGCCTGTTCACTTCAAAAGCTGGCCTCTGAAAATATAGGTAAAGCTATTCATAAACTGTTTTAGATCCATCGAAACATTATACATGCCCAAGCCAGTGAACCCATTTGAAAAGCCAGAGATAAGAGCTAAACATCTCAATAAAACAGACGCATATTATAAGTGTATTTCCTCACACAAGCAGTATCAAGCTATTGTACCTCAGGCGCTTAATGGCTGAAATCTCAAGAGTTCATGTCTTCATCAATCACCATCTGAATATGTTTCTTCAGTTTTCTCCTTCGATGGTTACGTCCCCTGCA

mRNA sequence

TGGATAAAATTCCAACTATCTGATCGACTCCGCTGCAGCCGCGTCAAGATGAAGAACAATCCACATAATCATGAAAACAACTACTGGAGTCCAGTCAACTTCGATCCTGGCCGTTGATTTGAAATGGATGTAACATTTTTTGTATTTATTAATCATCTGGAAAATATCACAGGAGTTTGACGAAGCGACGAAATTTATGTTCAAATCAACTACTGGAGCAAAGGATTGTTGAGGAAATGGTGTTTTCCGAGCGGTCAACTTTATCCAAGCCTATCGCTTCCAACTCCATTTATCTATGGTACATCGCCTTCTCTCTCGACGATTCTTTGTTGTTGCTATTGGTTGTTTTTCACTCTATTGTTAGCTTCGCCGCCGATGGGATTCTTCCTGCTGTTATTCTGATCGGAGCTTCATTTGGTTGGCCTCTGAGTGTTGGCGATTCGTGAAGTGAGTTTAGATCGTCTCTCCGAGTTCCGGGTTTGATTTCCAAAGATGGCTGTTCTTCTTGTTATGTCATCGATAATAGCGTCCGTCATGGGCGTGTTGCTGGCCCTATCGCCAAGAGTATATGCTCATGAGTTTATGATGGAATCAGGGGTTTCAAGTGCTCATCAGATTCCTTACTCGGTTTCGGATGGAATCAGGTGTTTCAAATGCAACTTTTTTGCAGATGATATTTCAGTTAAAACATCAAGGAAACTGTTAAGGGAAGAAAGGGAGGAGGAATACACTAAGCTTAAAGATGGTGATTATCCTCATCATAGAGCGAAACAGGATGGGAAATCTTTGGCAACCTCCCAAACTATTGGAATGGTAGTGACTGGAACATTCGTTTTATGTTTTGGTGTTCTCTGTCCTTGTTTTTATCGAAAGAGGAGACGCACAGTTCAAAGAGGCATTGAAATAGAACAACCATCAGTTCATTCAGCCTCCATTGAAGTGATGAACCCTGCTCCTGAAAAGGTTCCGGCCAGTCCACTTCGAGTACCACCTAGTCCTAGATATTCCCCATCTCCCAGATTCAAACGCCTTGGATCTGTACATCTCAACATGAGCCAAGTAGCCAGAGCAACTCAAGATTTCTCCCCAGCTCTGCGCATAGGCGAAGGAGGATATGTAAAATCTCAAACCCTTGTTTCTGAGAATAGTAACAGCATCTCTAACACAATTATTTTGCAGGAACTGTTTGAGAATTCACGAACTGACTTTAGCAGTGAAGTAGAGTTACTGTCCAAAATTGATCATCGGAGTCTGGTTAAGCTGCTAGGTCATGTTGATCATGGAAATGAGCGCATTATCATTACGGAATATGTAGGAAATGGTACTCTTAGAGAACACTTGGACGGCGTGCATGGAAAAGTATTGGATTTTAATCAGAGACTTGAAATTGCCATTGACATTGCTCATGGACTAACTTACCTCCATATGTATGCAGAGAAGCAAATTATTCACAGAGATGTTAAGTCTACCAACATTCTTCTTACCGAAAACATGAGAGCAAAGGTGGCGGATTTTGGATTTGCAAGGCTTGGAACATTGAGTACAGATCAAACGCACATTTCAACTCAGGTGAAAGGAACTGTTGGTTACCTTGACCCAGAGTATATGAAGACTTATCAACTTACTACCAAGAGTGATGTCTACTCATTTGGGATTTTACTCGTAGAAATTCTAACAGGACGCCGTCCATTGGAAGTTAAGAGGCCTCCTGATGAGCGGGTCACAATAAGATGGGCTTTCAACAAGTATAGTGAAAACAAAATTCTGGAAACCTTAGATCCTTCAATGGAGGAAACTGTTGACGCAGAGATTGTCATCAAAATGTTCAAATTGGCAATCCAATGTGCAGCACCGGTACGAGCCGAACGACCAGACATGAAATTTGTTGGGGAGCAGCTGTGGTCAATAAGAGCAAACTATATGTCACAGAAGAAGGCAAGATTACCTTAAAAAGGTGTGTTGTGTTGGAAACTCCAGAGGTGACCCACTGTGATTTCATCGATTTACCATGTCATTTAGAAGTTCACCTCCCTTCTTCAGAGTCTTTCCACGTTTGATGCTTTACTTAATTACCCTGTCATTAGAGTTTTCACCTCCAACAGATATCATTCATCACTGTTTTAATAGGTGAATCACCTATTAATTTGGAGTTTTTCTTTTCCTTCAACTTCTCTAGAATTGAAGTCAACAACACTTGTGTACCATTGTAAAAAAGGGGGGTTATTCCCAATTTGATGGCAAATGATATTAAAAATTGATATTGCAATTGAAATGGTAAATAATGACAATAATTTTAATTTTGTATATAGTTGAAATTGTGTCGGAGATATTGTCACCATGTCTTCTTTCAATTTTTACCTTATTGCCATGTTAACTTCCATTCATCATTTCAATTGAGAAATATTGGGAAGTTTCCTTGGGATTACTAAAATATATTAATAGCCACCGGGCATGGCCTGTTCACTTCAAAAGCTGGCCTCTGAAAATATAGGTAAAGCTATTCATAAACTGTTTTAGATCCATCGAAACATTATACATGCCCAAGCCAGTGAACCCATTTGAAAAGCCAGAGATAAGAGCTAAACATCTCAATAAAACAGACGCATATTATAAGTGTATTTCCTCACACAAGCAGTATCAAGCTATTGTACCTCAGGCGCTTAATGGCTGAAATCTCAAGAGTTCATGTCTTCATCAATCACCATCTGAATATGTTTCTTCAGTTTTCTCCTTCGATGGTTACGTCCCCTGCA

Coding sequence (CDS)

ATGGCTGTTCTTCTTGTTATGTCATCGATAATAGCGTCCGTCATGGGCGTGTTGCTGGCCCTATCGCCAAGAGTATATGCTCATGAGTTTATGATGGAATCAGGGGTTTCAAGTGCTCATCAGATTCCTTACTCGGTTTCGGATGGAATCAGGTGTTTCAAATGCAACTTTTTTGCAGATGATATTTCAGTTAAAACATCAAGGAAACTGTTAAGGGAAGAAAGGGAGGAGGAATACACTAAGCTTAAAGATGGTGATTATCCTCATCATAGAGCGAAACAGGATGGGAAATCTTTGGCAACCTCCCAAACTATTGGAATGGTAGTGACTGGAACATTCGTTTTATGTTTTGGTGTTCTCTGTCCTTGTTTTTATCGAAAGAGGAGACGCACAGTTCAAAGAGGCATTGAAATAGAACAACCATCAGTTCATTCAGCCTCCATTGAAGTGATGAACCCTGCTCCTGAAAAGGTTCCGGCCAGTCCACTTCGAGTACCACCTAGTCCTAGATATTCCCCATCTCCCAGATTCAAACGCCTTGGATCTGTACATCTCAACATGAGCCAAGTAGCCAGAGCAACTCAAGATTTCTCCCCAGCTCTGCGCATAGGCGAAGGAGGATATGTAAAATCTCAAACCCTTGTTTCTGAGAATAGTAACAGCATCTCTAACACAATTATTTTGCAGGAACTGTTTGAGAATTCACGAACTGACTTTAGCAGTGAAGTAGAGTTACTGTCCAAAATTGATCATCGGAGTCTGGTTAAGCTGCTAGGTCATGTTGATCATGGAAATGAGCGCATTATCATTACGGAATATGTAGGAAATGGTACTCTTAGAGAACACTTGGACGGCGTGCATGGAAAAGTATTGGATTTTAATCAGAGACTTGAAATTGCCATTGACATTGCTCATGGACTAACTTACCTCCATATGTATGCAGAGAAGCAAATTATTCACAGAGATGTTAAGTCTACCAACATTCTTCTTACCGAAAACATGAGAGCAAAGGTGGCGGATTTTGGATTTGCAAGGCTTGGAACATTGAGTACAGATCAAACGCACATTTCAACTCAGGTGAAAGGAACTGTTGGTTACCTTGACCCAGAGTATATGAAGACTTATCAACTTACTACCAAGAGTGATGTCTACTCATTTGGGATTTTACTCGTAGAAATTCTAACAGGACGCCGTCCATTGGAAGTTAAGAGGCCTCCTGATGAGCGGGTCACAATAAGATGGGCTTTCAACAAGTATAGTGAAAACAAAATTCTGGAAACCTTAGATCCTTCAATGGAGGAAACTGTTGACGCAGAGATTGTCATCAAAATGTTCAAATTGGCAATCCAATGTGCAGCACCGGTACGAGCCGAACGACCAGACATGAAATTTGTTGGGGAGCAGCTGTGGTCAATAAGAGCAAACTATATGTCACAGAAGAAGGCAAGATTACCTTAA

Protein sequence

MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFADDISVKTSRKLLREEREEEYTKLKDGDYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVLCPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRLGSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSRTDFSSEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQKKARLP

Homology

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BLAST of Cp4.1LG04g09200.1 vs. ExPASy Swiss-Prot
Match: Q9ASQ5 (Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRCK3 PE=2 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.1e-125
Identity = 225/381 (59.06%), Postives = 286/381 (75.07%), Query Frame = 0


Query: 105 IGMVVTGTFVLCFGVLCPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLR 164
           + +  +G  +LC  + CPCF+++R+      +  E  SVH  S   M+P+ EK+P SP R
Sbjct: 125 VSIAGSGFLLLCCALCCPCFHKERKANSHEVLPKESNSVHQVSSFEMSPSSEKIPQSPFR 184

Query: 165 VPPSP--------RYSPSPRFKRLGSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVS 224
            PPSP        RY+ SPR  RLG ++L MSQ+  AT +F+ + +IGEGG+      V 
Sbjct: 185 APPSPSRVPQSPSRYAMSPRPSRLGPLNLTMSQINTATGNFADSHQIGEGGFGVVFKGVL 244

Query: 225 ENSNSISNTIILQELFENSRTDFSSEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGN 284
           ++   ++     +E FEN RT+F SEV+LLSKI HR+LVKLLG+VD G+ER+IITEYV N
Sbjct: 245 DDGQVVAIKRAKKEHFENLRTEFKSEVDLLSKIGHRNLVKLLGYVDKGDERLIITEYVRN 304

Query: 285 GTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRA 344
           GTLR+HLDG  G  L+FNQRLEI ID+ HGLTYLH YAE+QIIHRD+KS+NILLT++MRA
Sbjct: 305 GTLRDHLDGARGTKLNFNQRLEIVIDVCHGLTYLHSYAERQIIHRDIKSSNILLTDSMRA 364

Query: 345 KVADFGFARLGTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTG 404
           KVADFGFAR G   ++QTHI TQVKGTVGYLDPEYMKTY LT KSDVYSFGILLVEILTG
Sbjct: 365 KVADFGFARGGPTDSNQTHILTQVKGTVGYLDPEYMKTYHLTAKSDVYSFGILLVEILTG 424

Query: 405 RRPLEVKRPPDERVTIRWAFNKYSENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVR 464
           RRP+E KR PDER+T+RWAF+KY+E ++ E +DP+  E VD +I+ KMF LA QCAAP +
Sbjct: 425 RRPVEAKRLPDERITVRWAFDKYNEGRVFELVDPNARERVDEKILRKMFSLAFQCAAPTK 484

Query: 465 AERPDMKFVGEQLWSIRANYM 478
            ERPDM+ VG+QLW+IR++Y+
Sbjct: 485 KERPDMEAVGKQLWAIRSSYL 505

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BLAST of Cp4.1LG04g09200.1 vs. ExPASy Swiss-Prot
Match: Q8VZJ9 (Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CRCK2 PE=2 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 2.3e-79
Identity = 148/293 (50.51%), Postives = 200/293 (68.26%), Query Frame = 0


Query: 189 QVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSR---TDFSSEVEL 248
           ++  AT++FSP+ RIG+GG+     +   +  + +     + + ++ +    +F SE++ 
Sbjct: 111 EIYDATKNFSPSFRIGQGGFGTVYKVKLRDGKTFAVKRAKKSMHDDRQGADAEFMSEIQT 170

Query: 249 LSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAH 308
           L+++ H SLVK  G V H +E+I++ EYV NGTLR+HLD   GK LD   RL+IA D+AH
Sbjct: 171 LAQVTHLSLVKYYGFVVHNDEKILVVEYVANGTLRDHLDCKEGKTLDMATRLDIATDVAH 230

Query: 309 GLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLG-TLSTDQTHISTQVKGTV 368
            +TYLHMY +  IIHRD+KS+NILLTEN RAKVADFGFARL     +  TH+STQVKGT 
Sbjct: 231 AITYLHMYTQPPIIHRDIKSSNILLTENYRAKVADFGFARLAPDTDSGATHVSTQVKGTA 290

Query: 369 GYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSENKI 428
           GYLDPEY+ TYQLT KSDVYSFG+LLVE+LTGRRP+E+ R   ER+TIRWA  K++    
Sbjct: 291 GYLDPEYLTTYQLTEKSDVYSFGVLLVELLTGRRPIELSRGQKERITIRWAIKKFTSGDT 350

Query: 429 LETLDPSMEETVDAEIVI-KMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANY 477
           +  LDP +E+     + + K+ ++A QC AP R  RP MK   E LW IR +Y
Sbjct: 351 ISVLDPKLEQNSANNLALEKVLEMAFQCLAPHRRSRPSMKKCSEILWGIRKDY 403

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BLAST of Cp4.1LG04g09200.1 vs. ExPASy Swiss-Prot
Match: Q9FIL7 (Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CRCK1 PE=1 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 3.0e-79
Identity = 149/308 (48.38%), Postives = 211/308 (68.51%), Query Frame = 0


Query: 172 SPSPRFKRLGSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQEL 231
           S S +    G V  +  ++ RAT +FS   +IGEGG+      V +        + ++  
Sbjct: 122 SSSSKSWHQGPVIFSFGELQRATANFSSVHQIGEGGF----GTVFKGKLDDGTIVAIKRA 181

Query: 232 FENS-----RTDFSSEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGV 291
            +N+       +F +E+  LSKI+H +LVKL G ++HG+E++I+ EYV NG LREHLDG+
Sbjct: 182 RKNNYGKSWLLEFKNEIYTLSKIEHMNLVKLYGFLEHGDEKVIVVEYVANGNLREHLDGL 241

Query: 292 HGKVLDFNQRLEIAIDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARL 351
            G  L+  +RLEIAID+AH LTYLH Y +  IIHRD+K++NIL+T  +RAKVADFGFARL
Sbjct: 242 RGNRLEMAERLEIAIDVAHALTYLHTYTDSPIIHRDIKASNILITNKLRAKVADFGFARL 301

Query: 352 GTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPP 411
            +     THISTQVKG+ GY+DP+Y++T+QLT KSDVYSFG+LLVEILTGRRP+E+KRP 
Sbjct: 302 VSEDLGATHISTQVKGSAGYVDPDYLRTFQLTDKSDVYSFGVLLVEILTGRRPIELKRPR 361

Query: 412 DERVTIRWAFNKYSENKILETLDPSMEETVDA-EIVIKMFKLAIQCAAPVRAERPDMKFV 471
            +R+T++WA  +  +++ +  +DP ++    A E+  KM +LA +C  P RA RP MK +
Sbjct: 362 KDRLTVKWALRRLKDDEAVLIMDPFLKRNRAAIEVAEKMLRLASECVTPTRATRPAMKGI 421

Query: 472 GEQLWSIR 474
            E+LW+IR
Sbjct: 422 AEKLWAIR 425

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BLAST of Cp4.1LG04g09200.1 vs. ExPASy Swiss-Prot
Match: O80623 (Probable receptor-like protein kinase At2g39360 OS=Arabidopsis thaliana OX=3702 GN=At2g39360 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.4e-52
Identity = 121/301 (40.20%), Postives = 179/301 (59.47%), Query Frame = 0


Query: 187 MSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSR---TDFSSEV 246
           ++ +  AT DF  +L IG GG+ K    V  +   ++    ++     SR    +F +EV
Sbjct: 477 LALIKEATDDFDESLVIGVGGFGKVYKGVLRDKTEVA----VKRGAPQSRQGLAEFKTEV 536

Query: 247 ELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKV-LDFNQRLEIAID 306
           E+L++  HR LV L+G+ D  +E II+ EY+  GTL++HL  +  K  L + QRLEI + 
Sbjct: 537 EMLTQFRHRHLVSLIGYCDENSEMIIVYEYMEKGTLKDHLYDLDDKPRLSWRQRLEICVG 596

Query: 307 IAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQVKG 366
            A GL YLH  + + IIHRDVKS NILL +N  AKVADFG ++ G    DQTH+ST VKG
Sbjct: 597 AARGLHYLHTGSTRAIIHRDVKSANILLDDNFMAKVADFGLSKTGP-DLDQTHVSTAVKG 656

Query: 367 TVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSEN 426
           + GYLDPEY+   QLT KSDVYSFG++++E++ GR  ++   P ++   I WA     + 
Sbjct: 657 SFGYLDPEYLTRQQLTEKSDVYSFGVVMLEVVCGRPVIDPSLPREKVNLIEWAMKLVKKG 716

Query: 427 KILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQKKA 484
           K+ + +DP +   V  E V K  ++  +C +    ERP M   G+ LW++   +M Q +A
Sbjct: 717 KLEDIIDPFLVGKVKLEEVKKYCEVTEKCLSQNGIERPAM---GDLLWNL--EFMLQVQA 767

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BLAST of Cp4.1LG04g09200.1 vs. ExPASy Swiss-Prot
Match: Q9FID9 (Probable receptor-like protein kinase At5g38990 OS=Arabidopsis thaliana OX=3702 GN=At5g38990 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.0e-51
Identity = 113/287 (39.37%), Postives = 167/287 (58.19%), Query Frame = 0


Query: 189 QVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSRTDFSSEVELLSK 248
           ++  AT DF   L IG GG+        +   ++     L+        +F +E+E+LSK
Sbjct: 517 EIKSATNDFEEKLIIGVGGFGSVYKGRIDGGATLVAVKRLEITSNQGAKEFDTELEMLSK 576

Query: 249 IDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHL---DGVHGKVLDFNQRLEIAIDIAH 308
           + H  LV L+G+ D  NE +++ EY+ +GTL++HL   D      L + +RLEI I  A 
Sbjct: 577 LRHVHLVSLIGYCDDDNEMVLVYEYMPHGTLKDHLFRRDKASDPPLSWKRRLEICIGAAR 636

Query: 309 GLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQVKGTVG 368
           GL YLH  A+  IIHRD+K+TNILL EN  AKV+DFG +R+G  S  QTH+ST VKGT G
Sbjct: 637 GLQYLHTGAKYTIIHRDIKTTNILLDENFVAKVSDFGLSRVGPTSASQTHVSTVVKGTFG 696

Query: 369 YLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSENKIL 428
           YLDPEY +   LT KSDVYSFG++L+E+L  R       PP++   IRW  + +++  + 
Sbjct: 697 YLDPEYYRRQILTEKSDVYSFGVVLLEVLCCRPIRMQSVPPEQADLIRWVKSNFNKRTVD 756

Query: 429 ETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSI 473
           + +D  +   + +  + K  ++AI+C      ERP M  V   +W++
Sbjct: 757 QIIDSDLTADITSTSMEKFCEIAIRCVQDRGMERPPMNDV---VWAL 800

[Close]

BLAST of Cp4.1LG04g09200.1 vs. NCBI nr
Match: XP_023530026.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita pepo subsp. pepo] >XP_023530027.1 calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita pepo subsp. pepo] >XP_023530028.1 calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 465/485 (95.88%), Postives = 470/485 (96.91%), Query Frame = 0


Query: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFAD 60
           MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFAD
Sbjct: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFAD 60

Query: 61  DISVKTSRKLLREEREEEYTKLKDGDYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVL 120
           DISVKTSRKLLREEREEEYTKLKDGDYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVL
Sbjct: 61  DISVKTSRKLLREEREEEYTKLKDGDYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVL 120

Query: 121 CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL 180
           CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL
Sbjct: 121 CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL 180

Query: 181 GSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSRTDFS 240
           GSVHLNMSQVARATQDFSPALRIGEGG+        EN + ++     +ELFENSRTDFS
Sbjct: 181 GSVHLNMSQVARATQDFSPALRIGEGGFGTVYKAQLENGHVVAIKRSKKELFENSRTDFS 240

Query: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA 300
           SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA
Sbjct: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA 300

Query: 301 IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV 360
           IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV
Sbjct: 301 IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV 360

Query: 361 KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS 420
           KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS
Sbjct: 361 KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS 420

Query: 421 ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK 480
           ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK
Sbjct: 421 ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK 480

Query: 481 KARLP 485
           KARLP
Sbjct: 481 KARLP 485

[Close]

BLAST of Cp4.1LG04g09200.1 vs. NCBI nr
Match: XP_022930295.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita moschata] >XP_022930303.1 calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita moschata] >XP_022930311.1 calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita moschata])

HSP 1 Score: 883 bits (2282), Expect = 0.0
Identity = 455/485 (93.81%), Postives = 462/485 (95.26%), Query Frame = 0


Query: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFAD 60
           MA LLVMSSIIA+VMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIR FKCNFFA 
Sbjct: 1   MADLLVMSSIIAAVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRGFKCNFFAG 60

Query: 61  DISVKTSRKLLREEREEEYTKLKDGDYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVL 120
           DISVKTSRKLLREEREEEYT LKDGDYPH R KQDGKSLAT Q IGMVVTGTFVLCFGVL
Sbjct: 61  DISVKTSRKLLREEREEEYTNLKDGDYPHRREKQDGKSLATPQNIGMVVTGTFVLCFGVL 120

Query: 121 CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL 180
           CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL
Sbjct: 121 CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL 180

Query: 181 GSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSRTDFS 240
           GSVHLNMSQVARATQDFSPALRIGEGG+        EN + ++     +ELFENSRTDFS
Sbjct: 181 GSVHLNMSQVARATQDFSPALRIGEGGFGTVYKAQLENGHVVAIKRSKKELFENSRTDFS 240

Query: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA 300
           SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGK+LDFNQRLEIA
Sbjct: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKILDFNQRLEIA 300

Query: 301 IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV 360
           IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV
Sbjct: 301 IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV 360

Query: 361 KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS 420
           KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS
Sbjct: 361 KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS 420

Query: 421 ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK 480
           ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK
Sbjct: 421 ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK 480

Query: 481 KARLP 485
           KARLP
Sbjct: 481 KARLP 485

[Close]

BLAST of Cp4.1LG04g09200.1 vs. NCBI nr
Match: XP_023002661.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita maxima] >XP_023002670.1 calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita maxima])

HSP 1 Score: 863 bits (2229), Expect = 4.43e-314
Identity = 448/485 (92.37%), Postives = 456/485 (94.02%), Query Frame = 0


Query: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFAD 60
           MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSA+QIPYSVS GIR FKCNFFA 
Sbjct: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSANQIPYSVSYGIRGFKCNFFAA 60

Query: 61  DISVKTSRKLLREEREEEYTKLKDGDYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVL 120
           DISVKTSRKLLREEREEEYT LKD DYPHHR KQDGKSLAT Q IGMVVTGTFVLC GVL
Sbjct: 61  DISVKTSRKLLREEREEEYTNLKDCDYPHHREKQDGKSLATPQNIGMVVTGTFVLCLGVL 120

Query: 121 CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL 180
           CPCFYRKRR TVQRGI+IEQ SVHSASIEVMNPA EKVPASPLRVPPSPRYSPSPRFKRL
Sbjct: 121 CPCFYRKRRHTVQRGIKIEQQSVHSASIEVMNPASEKVPASPLRVPPSPRYSPSPRFKRL 180

Query: 181 GSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSRTDFS 240
           GSVHLNMSQVARATQDFSPALRIGEGG+        EN + ++     +ELFENSRTDFS
Sbjct: 181 GSVHLNMSQVARATQDFSPALRIGEGGFGTVYKAQLENGHVVAIKRSKKELFENSRTDFS 240

Query: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA 300
           SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA
Sbjct: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA 300

Query: 301 IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV 360
           IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV
Sbjct: 301 IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV 360

Query: 361 KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS 420
           KGT+GYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS
Sbjct: 361 KGTIGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS 420

Query: 421 ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK 480
           ENKILETLD SMEETVDAEIVIKMFKLAIQCAAPVRAERPDMK VGEQLWSIRANYMSQK
Sbjct: 421 ENKILETLDSSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKLVGEQLWSIRANYMSQK 480

Query: 481 KARLP 485
           KARLP
Sbjct: 481 KARLP 485

[Close]

BLAST of Cp4.1LG04g09200.1 vs. NCBI nr
Match: KAG7021759.1 (Calmodulin-binding receptor-like cytoplasmic kinase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 834 bits (2154), Expect = 4.57e-301
Identity = 452/571 (79.16%), Postives = 455/571 (79.68%), Query Frame = 0


Query: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFAD 60
           MAVLLVMSSIIA+VMGVLLA SPRVYAHEFMMESGVSSAHQIPYSVSDGIR FKCNFFAD
Sbjct: 1   MAVLLVMSSIIAAVMGVLLAQSPRVYAHEFMMESGVSSAHQIPYSVSDGIRGFKCNFFAD 60

Query: 61  DISVKTSRKLLREEREEEYTKLKDGDYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVL 120
           DISVKTSRKLLREEREEEYT LKDGDYPH R KQDGKSLAT Q IGMVVTGTFVLCFGVL
Sbjct: 61  DISVKTSRKLLREEREEEYTNLKDGDYPHRREKQDGKSLATPQNIGMVVTGTFVLCFGVL 120

Query: 121 CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL 180
           CPCFYRKRR TVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL
Sbjct: 121 CPCFYRKRRHTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL 180

Query: 181 GSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSRTDFS 240
           GSVHLNMSQVARATQDFSPALRIGEGG+        EN +         ELFENSRTDFS
Sbjct: 181 GSVHLNMSQVARATQDFSPALRIGEGGFGTVYKAQLENGH---------ELFENSRTDFS 240

Query: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA 300
           SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA
Sbjct: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA 300

Query: 301 IDIAHGLTYLHMYA---------------------------------------------- 360
           IDIAHGLTYLHMYA                                              
Sbjct: 301 IDIAHGLTYLHMYAGPIPSLADVVHFGLFLLDFSLKVLKRGPASLLILVPFFNRCETPQF 360

Query: 361 --------------------------------------------EKQIIHRDVKSTNILL 420
                                                       EKQIIHRDVKSTNILL
Sbjct: 361 TTFEASDLASTPPCVHPSSRLSLLVGMSPDVWLCQLTFFFLLTIEKQIIHRDVKSTNILL 420

Query: 421 TENMRAKVADFGFARLGTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILL 480
           TENMRAKVADFGFARLGTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILL
Sbjct: 421 TENMRAKVADFGFARLGTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILL 480

[Close]

BLAST of Cp4.1LG04g09200.1 vs. NCBI nr
Match: XP_038879737.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 [Benincasa hispida])

HSP 1 Score: 729 bits (1881), Expect = 1.75e-260
Identity = 394/523 (75.33%), Postives = 429/523 (82.03%), Query Frame = 0


Query: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDG----------- 60
           MA LLVMSSIIAS+  VLL  SP V+A EFMMESGVS  +QIPYSVS G           
Sbjct: 1   MAFLLVMSSIIASITAVLLMQSPGVFASEFMMESGVSRGNQIPYSVSKGCERYFMNGIPM 60

Query: 61  ---IRCFKCNFF------------ADD---------ISVKTSRKLLREEREEEYTKLKDG 120
              + C    F             +DD         I VKTSRKLL+EER++EY  LKDG
Sbjct: 61  DKIVFCKLLQFLRGYPGIFENSRGSDDCCWDNSFSGIRVKTSRKLLKEERKDEYINLKDG 120

Query: 121 DYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVLCPCFYRKRRRTVQRGIEIEQPSVHS 180
            Y H RAK+D KS ATS+ IG+ VTGTFVLC GVLCPCFYRKRRR+VQ+  EIEQ SVH 
Sbjct: 121 QYLHQRAKEDPKSFATSKNIGIAVTGTFVLCCGVLCPCFYRKRRRSVQKLSEIEQQSVHL 180

Query: 181 AS-IEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRLGSVHLNMSQVARATQDFSPALRIG 240
           AS IE MN  PEK+ ASPLRVPPSPRYSPSP+FKRLGSVHLNMSQVARATQ+FSPALRIG
Sbjct: 181 ASTIEAMNSTPEKILASPLRVPPSPRYSPSPKFKRLGSVHLNMSQVARATQEFSPALRIG 240

Query: 241 EGGYVKSQTLVSENSNSISNTIILQELFENSRTDFSSEVELLSKIDHRSLVKLLGHVDHG 300
           EGG+        E+   ++     +E FENSRTDF SEVELLSKIDHRSLVKLLG+VDHG
Sbjct: 241 EGGFGTVYKAQLEDGQIVAIKRAKKEQFENSRTDFGSEVELLSKIDHRSLVKLLGYVDHG 300

Query: 301 NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHMYAEKQIIHRDVK 360
           NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLH+YAEKQIIHRDVK
Sbjct: 301 NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDVK 360

Query: 361 STNILLTENMRAKVADFGFARLGTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVY 420
           STNILLTE+MRAKVADFGFARLGTL T+QTHISTQVKGTVGYLDPEYMKTYQLTTKSDVY
Sbjct: 361 STNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVY 420

Query: 421 SFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSENKILETLDPSMEETVDAEIVIKM 480
           SFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSE+KI+ETLDP MEETVDA+IV+KM
Sbjct: 421 SFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSEDKIVETLDPLMEETVDADIVVKM 480

Query: 481 FKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQKK--ARLP 485
           F+LAIQCAAPVRA+RPDMK VGEQLW+IRA+Y+SQKK  AR+P
Sbjct: 481 FELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARVP 523

[Close]

BLAST of Cp4.1LG04g09200.1 vs. ExPASy TrEMBL
Match: A0A6J1EUR2 (calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita moschata OX=3662 GN=LOC111436788 PE=3 SV=1)

HSP 1 Score: 883 bits (2282), Expect = 0.0
Identity = 455/485 (93.81%), Postives = 462/485 (95.26%), Query Frame = 0


Query: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFAD 60
           MA LLVMSSIIA+VMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIR FKCNFFA 
Sbjct: 1   MADLLVMSSIIAAVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRGFKCNFFAG 60

Query: 61  DISVKTSRKLLREEREEEYTKLKDGDYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVL 120
           DISVKTSRKLLREEREEEYT LKDGDYPH R KQDGKSLAT Q IGMVVTGTFVLCFGVL
Sbjct: 61  DISVKTSRKLLREEREEEYTNLKDGDYPHRREKQDGKSLATPQNIGMVVTGTFVLCFGVL 120

Query: 121 CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL 180
           CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL
Sbjct: 121 CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL 180

Query: 181 GSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSRTDFS 240
           GSVHLNMSQVARATQDFSPALRIGEGG+        EN + ++     +ELFENSRTDFS
Sbjct: 181 GSVHLNMSQVARATQDFSPALRIGEGGFGTVYKAQLENGHVVAIKRSKKELFENSRTDFS 240

Query: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA 300
           SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGK+LDFNQRLEIA
Sbjct: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKILDFNQRLEIA 300

Query: 301 IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV 360
           IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV
Sbjct: 301 IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV 360

Query: 361 KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS 420
           KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS
Sbjct: 361 KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS 420

Query: 421 ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK 480
           ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK
Sbjct: 421 ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK 480

Query: 481 KARLP 485
           KARLP
Sbjct: 481 KARLP 485

[Close]

BLAST of Cp4.1LG04g09200.1 vs. ExPASy TrEMBL
Match: A0A6J1KLX6 (calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita maxima OX=3661 GN=LOC111496449 PE=3 SV=1)

HSP 1 Score: 863 bits (2229), Expect = 2.15e-314
Identity = 448/485 (92.37%), Postives = 456/485 (94.02%), Query Frame = 0


Query: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFAD 60
           MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSA+QIPYSVS GIR FKCNFFA 
Sbjct: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSANQIPYSVSYGIRGFKCNFFAA 60

Query: 61  DISVKTSRKLLREEREEEYTKLKDGDYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVL 120
           DISVKTSRKLLREEREEEYT LKD DYPHHR KQDGKSLAT Q IGMVVTGTFVLC GVL
Sbjct: 61  DISVKTSRKLLREEREEEYTNLKDCDYPHHREKQDGKSLATPQNIGMVVTGTFVLCLGVL 120

Query: 121 CPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRL 180
           CPCFYRKRR TVQRGI+IEQ SVHSASIEVMNPA EKVPASPLRVPPSPRYSPSPRFKRL
Sbjct: 121 CPCFYRKRRHTVQRGIKIEQQSVHSASIEVMNPASEKVPASPLRVPPSPRYSPSPRFKRL 180

Query: 181 GSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSRTDFS 240
           GSVHLNMSQVARATQDFSPALRIGEGG+        EN + ++     +ELFENSRTDFS
Sbjct: 181 GSVHLNMSQVARATQDFSPALRIGEGGFGTVYKAQLENGHVVAIKRSKKELFENSRTDFS 240

Query: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA 300
           SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA
Sbjct: 241 SEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIA 300

Query: 301 IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV 360
           IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV
Sbjct: 301 IDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQV 360

Query: 361 KGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS 420
           KGT+GYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS
Sbjct: 361 KGTIGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYS 420

Query: 421 ENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQK 480
           ENKILETLD SMEETVDAEIVIKMFKLAIQCAAPVRAERPDMK VGEQLWSIRANYMSQK
Sbjct: 421 ENKILETLDSSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKLVGEQLWSIRANYMSQK 480

Query: 481 KARLP 485
           KARLP
Sbjct: 481 KARLP 485

[Close]

BLAST of Cp4.1LG04g09200.1 vs. ExPASy TrEMBL
Match: A0A6J1H8N3 (calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita moschata OX=3662 GN=LOC111461507 PE=4 SV=1)

HSP 1 Score: 716 bits (1848), Expect = 8.87e-256
Identity = 383/523 (73.23%), Postives = 426/523 (81.45%), Query Frame = 0


Query: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFAD 60
           MA LLV+S ++AS+  VLL  SP V+A EFMMESGVSSA+QIPYSVSDG   +  N    
Sbjct: 1   MAFLLVVSCVVASITAVLLVQSPLVFASEFMMESGVSSANQIPYSVSDGCERYFMNGIPM 60

Query: 61  DIS-----------------------------------VKTSRKLLREEREEEYTKLKDG 120
           D S                                   VK SRKLL+EER+EE   +KDG
Sbjct: 61  DKSMFCKLLQFLHGYPRIFEDSWGSDGCRWDSSFSGMRVKASRKLLKEERKEEDANVKDG 120

Query: 121 DYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVLCPCFYRKRRRTVQRGIEIEQPSVHS 180
            Y H +AK+D KS AT Q IG+VVTGTFVLC GVLCPCFY+KRRRTVQR ++ EQ SVH 
Sbjct: 121 KYRHRKAKEDPKSFATPQNIGIVVTGTFVLCCGVLCPCFYQKRRRTVQRALDKEQQSVHL 180

Query: 181 AS-IEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRLGSVHLNMSQVARATQDFSPALRIG 240
           AS IEVMN APEK+ ASPLRVPPSPRYSPSP+FKRLGSV LNMSQVA+ATQDFSPALRIG
Sbjct: 181 ASTIEVMNSAPEKILASPLRVPPSPRYSPSPKFKRLGSVRLNMSQVAKATQDFSPALRIG 240

Query: 241 EGGYVKSQTLVSENSNSISNTIILQELFENSRTDFSSEVELLSKIDHRSLVKLLGHVDHG 300
           EGG+        E+ + ++     +ELFENSRTDF SEVELLSKIDHRSLVKLLG+VDHG
Sbjct: 241 EGGFGTVYKARLEDGHIVAIKRAKKELFENSRTDFGSEVELLSKIDHRSLVKLLGYVDHG 300

Query: 301 NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHMYAEKQIIHRDVK 360
           NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLH+YAEKQIIHRDVK
Sbjct: 301 NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDVK 360

Query: 361 STNILLTENMRAKVADFGFARLGTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVY 420
           S+NILLTE MRAKVADFGFARLGTL ++QTHISTQVKGTVGYLDP+YMKTYQLT KSDVY
Sbjct: 361 SSNILLTETMRAKVADFGFARLGTLGSEQTHISTQVKGTVGYLDPDYMKTYQLTPKSDVY 420

Query: 421 SFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSENKILETLDPSMEETVDAEIVIKM 480
           SFGILLVEILTGRRPLEVK+PP+ERVTI+WAFNKYSE+KILETLDP MEET++A+IV+KM
Sbjct: 421 SFGILLVEILTGRRPLEVKKPPEERVTIKWAFNKYSEDKILETLDPLMEETLEADIVLKM 480

Query: 481 FKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQKK--ARLP 485
           F+LAIQCAAPVRA+RPDMK VGEQLW+IRA Y+SQKK  ARLP
Sbjct: 481 FELAIQCAAPVRADRPDMKLVGEQLWAIRAEYISQKKEKARLP 523

[Close]

BLAST of Cp4.1LG04g09200.1 vs. ExPASy TrEMBL
Match: A0A6J1C0H6 (calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Momordica charantia OX=3673 GN=LOC111007158 PE=4 SV=1)

HSP 1 Score: 714 bits (1844), Expect = 3.60e-255
Identity = 380/523 (72.66%), Postives = 426/523 (81.45%), Query Frame = 0


Query: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVS-SAHQIPYSVSDGIRCFKCNFFA 60
           MA LLV+SSI+ASVMG+LL  SPRV A EFMMESGVS S +QIPY VSDG   +  N  +
Sbjct: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60

Query: 61  DD-----------------------------------ISVKTSRKLLREEREEEYTKLKD 120
            D                                   +SVK SRKLL+EE++EEY  LKD
Sbjct: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120

Query: 121 GDYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVLCPCFYRKRRRTVQRGIEIEQPSVH 180
           G      AK+D KS A+ + IG+V+TGTFVLC GVLCPCFYRKRRRTVQ+ +  EQ SVH
Sbjct: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180

Query: 181 SASIEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRLGSVHLNMSQVARATQDFSPALRIG 240
           S+SIEVM+ APEK+P SPLRVPPSPRYSPSP+ KRLGSVHLNMSQV RAT+DFSP+LRIG
Sbjct: 181 SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRIG 240

Query: 241 EGGYVKSQTLVSENSNSISNTIILQELFENSRTDFSSEVELLSKIDHRSLVKLLGHVDHG 300
           EGG+        E+ + ++     +E+FENSRTDFSSEVELL+KIDHRSLVKLLG+VDHG
Sbjct: 241 EGGFGTVYKAQLEDGHIVAVKRAKKEIFENSRTDFSSEVELLAKIDHRSLVKLLGYVDHG 300

Query: 301 NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHMYAEKQIIHRDVK 360
           NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLH+YAEKQIIHRDVK
Sbjct: 301 NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDVK 360

Query: 361 STNILLTENMRAKVADFGFARLGTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVY 420
           STNILLTE+MRAKVADFGFARLGTL T+QTHISTQVKGTVGYLDPEYMKTYQLTTKSDVY
Sbjct: 361 STNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVY 420

Query: 421 SFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSENKILETLDPSMEETVDAEIVIKM 480
           SFGILLVEILTGRRPLE KRPP+ERVTIRWAF KYS++KILETLDP ++ETVD EIV+KM
Sbjct: 421 SFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVVKM 480

Query: 481 FKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQKK--ARLP 485
           F+LAIQCAAPVRA+RPDMK VGEQLW+IRA+Y+SQKK  AR P
Sbjct: 481 FELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 523

[Close]

BLAST of Cp4.1LG04g09200.1 vs. ExPASy TrEMBL
Match: A0A6J1JK63 (calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita maxima OX=3661 GN=LOC111485167 PE=4 SV=1)

HSP 1 Score: 714 bits (1843), Expect = 5.11e-255
Identity = 382/523 (73.04%), Postives = 425/523 (81.26%), Query Frame = 0


Query: 1   MAVLLVMSSIIASVMGVLLALSPRVYAHEFMMESGVSSAHQIPYSVSDGIRCFKCNFFAD 60
           M+ LLV+S I+AS+  V+L  SP V+A EFMMESGVSSA+QIPYSVSDG   +  N    
Sbjct: 1   MSFLLVVSCIVASITAVMLVQSPLVFASEFMMESGVSSANQIPYSVSDGFERYFMNGIPM 60

Query: 61  DIS-----------------------------------VKTSRKLLREEREEEYTKLKDG 120
           D S                                   VK SRKLL+EER+EE    KDG
Sbjct: 61  DKSMFCKLLQFLHGYPRILEDSWGSDGCRWDSSFSGMRVKASRKLLKEERKEEDANFKDG 120

Query: 121 DYPHHRAKQDGKSLATSQTIGMVVTGTFVLCFGVLCPCFYRKRRRTVQRGIEIEQPSVHS 180
            Y H +AK+D KS AT Q IG+VVTGTFVLC GVLCPCFY+KRRRTVQR ++ EQ SVH 
Sbjct: 121 QYRHWKAKEDPKSFATPQNIGIVVTGTFVLCCGVLCPCFYQKRRRTVQRALDKEQQSVHL 180

Query: 181 AS-IEVMNPAPEKVPASPLRVPPSPRYSPSPRFKRLGSVHLNMSQVARATQDFSPALRIG 240
           AS IEVMN APEK+ ASPLRVPPSPRYSPSP+FKRLGSV LNMSQVA+ATQDFSPALRIG
Sbjct: 181 ASTIEVMNSAPEKILASPLRVPPSPRYSPSPKFKRLGSVRLNMSQVAKATQDFSPALRIG 240

Query: 241 EGGYVKSQTLVSENSNSISNTIILQELFENSRTDFSSEVELLSKIDHRSLVKLLGHVDHG 300
           EGG+        E+ + ++     +ELFENSRTDF SEVELLSKIDHRSLVKLLG+VDHG
Sbjct: 241 EGGFGTVYKARLEDGHIVAIKRAKKELFENSRTDFGSEVELLSKIDHRSLVKLLGYVDHG 300

Query: 301 NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHMYAEKQIIHRDVK 360
           NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLH+YAEKQIIHRDVK
Sbjct: 301 NERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDVK 360

Query: 361 STNILLTENMRAKVADFGFARLGTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVY 420
           S+NILLTE MRAKVADFGFARLGTL ++QTHISTQVKGTVGYLDP+YMKTYQLT KSDVY
Sbjct: 361 SSNILLTETMRAKVADFGFARLGTLGSEQTHISTQVKGTVGYLDPDYMKTYQLTPKSDVY 420

Query: 421 SFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSENKILETLDPSMEETVDAEIVIKM 480
           SFGILLVEILTGRRPLEVK+PP+ERVTI+WAFNKYSE+KILETLDP MEET++AEIV+KM
Sbjct: 421 SFGILLVEILTGRRPLEVKKPPEERVTIKWAFNKYSEDKILETLDPLMEETLEAEIVLKM 480

Query: 481 FKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQKK--ARLP 485
           F+LAIQCAAPVRA+RPDMK VGEQLW+IRA Y+SQKK  AR+P
Sbjct: 481 FELAIQCAAPVRADRPDMKLVGEQLWAIRAEYISQKKEKARVP 523

[Close]

BLAST of Cp4.1LG04g09200.1 vs. TAIR 10
Match: AT2G11520.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 )

HSP 1 Score: 450.7 bits (1158), Expect = 1.5e-126
Identity = 225/381 (59.06%), Postives = 286/381 (75.07%), Query Frame = 0


Query: 105 IGMVVTGTFVLCFGVLCPCFYRKRRRTVQRGIEIEQPSVHSASIEVMNPAPEKVPASPLR 164
           + +  +G  +LC  + CPCF+++R+      +  E  SVH  S   M+P+ EK+P SP R
Sbjct: 125 VSIAGSGFLLLCCALCCPCFHKERKANSHEVLPKESNSVHQVSSFEMSPSSEKIPQSPFR 184

Query: 165 VPPSP--------RYSPSPRFKRLGSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVS 224
            PPSP        RY+ SPR  RLG ++L MSQ+  AT +F+ + +IGEGG+      V 
Sbjct: 185 APPSPSRVPQSPSRYAMSPRPSRLGPLNLTMSQINTATGNFADSHQIGEGGFGVVFKGVL 244

Query: 225 ENSNSISNTIILQELFENSRTDFSSEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGN 284
           ++   ++     +E FEN RT+F SEV+LLSKI HR+LVKLLG+VD G+ER+IITEYV N
Sbjct: 245 DDGQVVAIKRAKKEHFENLRTEFKSEVDLLSKIGHRNLVKLLGYVDKGDERLIITEYVRN 304

Query: 285 GTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRA 344
           GTLR+HLDG  G  L+FNQRLEI ID+ HGLTYLH YAE+QIIHRD+KS+NILLT++MRA
Sbjct: 305 GTLRDHLDGARGTKLNFNQRLEIVIDVCHGLTYLHSYAERQIIHRDIKSSNILLTDSMRA 364

Query: 345 KVADFGFARLGTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTG 404
           KVADFGFAR G   ++QTHI TQVKGTVGYLDPEYMKTY LT KSDVYSFGILLVEILTG
Sbjct: 365 KVADFGFARGGPTDSNQTHILTQVKGTVGYLDPEYMKTYHLTAKSDVYSFGILLVEILTG 424

Query: 405 RRPLEVKRPPDERVTIRWAFNKYSENKILETLDPSMEETVDAEIVIKMFKLAIQCAAPVR 464
           RRP+E KR PDER+T+RWAF+KY+E ++ E +DP+  E VD +I+ KMF LA QCAAP +
Sbjct: 425 RRPVEAKRLPDERITVRWAFDKYNEGRVFELVDPNARERVDEKILRKMFSLAFQCAAPTK 484

Query: 465 AERPDMKFVGEQLWSIRANYM 478
            ERPDM+ VG+QLW+IR++Y+
Sbjct: 485 KERPDMEAVGKQLWAIRSSYL 505

[Close]

BLAST of Cp4.1LG04g09200.1 vs. TAIR 10
Match: AT4G00330.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 )

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-80
Identity = 148/293 (50.51%), Postives = 200/293 (68.26%), Query Frame = 0


Query: 189 QVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSR---TDFSSEVEL 248
           ++  AT++FSP+ RIG+GG+     +   +  + +     + + ++ +    +F SE++ 
Sbjct: 111 EIYDATKNFSPSFRIGQGGFGTVYKVKLRDGKTFAVKRAKKSMHDDRQGADAEFMSEIQT 170

Query: 249 LSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAH 308
           L+++ H SLVK  G V H +E+I++ EYV NGTLR+HLD   GK LD   RL+IA D+AH
Sbjct: 171 LAQVTHLSLVKYYGFVVHNDEKILVVEYVANGTLRDHLDCKEGKTLDMATRLDIATDVAH 230

Query: 309 GLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLG-TLSTDQTHISTQVKGTV 368
            +TYLHMY +  IIHRD+KS+NILLTEN RAKVADFGFARL     +  TH+STQVKGT 
Sbjct: 231 AITYLHMYTQPPIIHRDIKSSNILLTENYRAKVADFGFARLAPDTDSGATHVSTQVKGTA 290

Query: 369 GYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSENKI 428
           GYLDPEY+ TYQLT KSDVYSFG+LLVE+LTGRRP+E+ R   ER+TIRWA  K++    
Sbjct: 291 GYLDPEYLTTYQLTEKSDVYSFGVLLVELLTGRRPIELSRGQKERITIRWAIKKFTSGDT 350

Query: 429 LETLDPSMEETVDAEIVI-KMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANY 477
           +  LDP +E+     + + K+ ++A QC AP R  RP MK   E LW IR +Y
Sbjct: 351 ISVLDPKLEQNSANNLALEKVLEMAFQCLAPHRRSRPSMKKCSEILWGIRKDY 403

[Close]

BLAST of Cp4.1LG04g09200.1 vs. TAIR 10
Match: AT5G58940.1 (calmodulin-binding receptor-like cytoplasmic kinase 1 )

HSP 1 Score: 297.4 bits (760), Expect = 2.1e-80
Identity = 149/308 (48.38%), Postives = 211/308 (68.51%), Query Frame = 0


Query: 172 SPSPRFKRLGSVHLNMSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQEL 231
           S S +    G V  +  ++ RAT +FS   +IGEGG+      V +        + ++  
Sbjct: 122 SSSSKSWHQGPVIFSFGELQRATANFSSVHQIGEGGF----GTVFKGKLDDGTIVAIKRA 181

Query: 232 FENS-----RTDFSSEVELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGV 291
            +N+       +F +E+  LSKI+H +LVKL G ++HG+E++I+ EYV NG LREHLDG+
Sbjct: 182 RKNNYGKSWLLEFKNEIYTLSKIEHMNLVKLYGFLEHGDEKVIVVEYVANGNLREHLDGL 241

Query: 292 HGKVLDFNQRLEIAIDIAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARL 351
            G  L+  +RLEIAID+AH LTYLH Y +  IIHRD+K++NIL+T  +RAKVADFGFARL
Sbjct: 242 RGNRLEMAERLEIAIDVAHALTYLHTYTDSPIIHRDIKASNILITNKLRAKVADFGFARL 301

Query: 352 GTLSTDQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPP 411
            +     THISTQVKG+ GY+DP+Y++T+QLT KSDVYSFG+LLVEILTGRRP+E+KRP 
Sbjct: 302 VSEDLGATHISTQVKGSAGYVDPDYLRTFQLTDKSDVYSFGVLLVEILTGRRPIELKRPR 361

Query: 412 DERVTIRWAFNKYSENKILETLDPSMEETVDA-EIVIKMFKLAIQCAAPVRAERPDMKFV 471
            +R+T++WA  +  +++ +  +DP ++    A E+  KM +LA +C  P RA RP MK +
Sbjct: 362 KDRLTVKWALRRLKDDEAVLIMDPFLKRNRAAIEVAEKMLRLASECVTPTRATRPAMKGI 421

Query: 472 GEQLWSIR 474
            E+LW+IR
Sbjct: 422 AEKLWAIR 425

[Close]

BLAST of Cp4.1LG04g09200.1 vs. TAIR 10
Match: AT2G39360.1 (Protein kinase superfamily protein )

HSP 1 Score: 208.8 bits (530), Expect = 1.0e-53
Identity = 121/301 (40.20%), Postives = 179/301 (59.47%), Query Frame = 0


Query: 187 MSQVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSR---TDFSSEV 246
           ++ +  AT DF  +L IG GG+ K    V  +   ++    ++     SR    +F +EV
Sbjct: 477 LALIKEATDDFDESLVIGVGGFGKVYKGVLRDKTEVA----VKRGAPQSRQGLAEFKTEV 536

Query: 247 ELLSKIDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHLDGVHGKV-LDFNQRLEIAID 306
           E+L++  HR LV L+G+ D  +E II+ EY+  GTL++HL  +  K  L + QRLEI + 
Sbjct: 537 EMLTQFRHRHLVSLIGYCDENSEMIIVYEYMEKGTLKDHLYDLDDKPRLSWRQRLEICVG 596

Query: 307 IAHGLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQVKG 366
            A GL YLH  + + IIHRDVKS NILL +N  AKVADFG ++ G    DQTH+ST VKG
Sbjct: 597 AARGLHYLHTGSTRAIIHRDVKSANILLDDNFMAKVADFGLSKTGP-DLDQTHVSTAVKG 656

Query: 367 TVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSEN 426
           + GYLDPEY+   QLT KSDVYSFG++++E++ GR  ++   P ++   I WA     + 
Sbjct: 657 SFGYLDPEYLTRQQLTEKSDVYSFGVVMLEVVCGRPVIDPSLPREKVNLIEWAMKLVKKG 716

Query: 427 KILETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSIRANYMSQKKA 484
           K+ + +DP +   V  E V K  ++  +C +    ERP M   G+ LW++   +M Q +A
Sbjct: 717 KLEDIIDPFLVGKVKLEEVKKYCEVTEKCLSQNGIERPAM---GDLLWNL--EFMLQVQA 767

[Close]

BLAST of Cp4.1LG04g09200.1 vs. TAIR 10
Match: AT5G39000.1 (Malectin/receptor-like protein kinase family protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.5e-52
Identity = 112/287 (39.02%), Postives = 164/287 (57.14%), Query Frame = 0


Query: 189 QVARATQDFSPALRIGEGGYVKSQTLVSENSNSISNTIILQELFENSRTDFSSEVELLSK 248
           ++  AT DF   L IG GG+        +   ++     L+        +F +E+E+LSK
Sbjct: 510 EIKSATNDFEDKLIIGVGGFGSVYKGQIDGGATLVAVKRLEITSNQGAKEFETELEMLSK 569

Query: 249 IDHRSLVKLLGHVDHGNERIIITEYVGNGTLREHL---DGVHGKVLDFNQRLEIAIDIAH 308
           + H  LV L+G+ D  NE +++ EY+ +GTL++HL   D      L + +RLEI I  A 
Sbjct: 570 LRHVHLVSLIGYCDEDNEMVLVYEYMPHGTLKDHLFRRDKTSDPPLSWKRRLEICIGAAR 629

Query: 309 GLTYLHMYAEKQIIHRDVKSTNILLTENMRAKVADFGFARLGTLSTDQTHISTQVKGTVG 368
           GL YLH  A+  IIHRD+K+TNILL EN   KV+DFG +R+G  S  QTH+ST VKGT G
Sbjct: 630 GLQYLHTGAKYTIIHRDIKTTNILLDENFVTKVSDFGLSRVGPTSASQTHVSTVVKGTFG 689

Query: 369 YLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSENKIL 428
           YLDPEY +   LT KSDVYSFG++L+E+L  R       PP++   IRW  + Y    + 
Sbjct: 690 YLDPEYYRRQVLTEKSDVYSFGVVLLEVLCCRPIRMQSVPPEQADLIRWVKSNYRRGTVD 749

Query: 429 ETLDPSMEETVDAEIVIKMFKLAIQCAAPVRAERPDMKFVGEQLWSI 473
           + +D  +   + +  + K  ++A++C      ERP M  V   +W++
Sbjct: 750 QIIDSDLSADITSTSLEKFCEIAVRCVQDRGMERPPMNDV---VWAL 793

The following BLAST results are available for this feature:

BLAST of Cp4.1LG04g09200.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ASQ5	2.1e-125	59.06	Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Arabidopsis thaliana OX...	[more]
Q8VZJ9	2.3e-79	50.51	Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Arabidopsis thaliana OX...	[more]
Q9FIL7	3.0e-79	48.38	Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Arabidopsis thaliana OX...	[more]
O80623	1.4e-52	40.20	Probable receptor-like protein kinase At2g39360 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9FID9	2.0e-51	39.37	Probable receptor-like protein kinase At5g38990 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Cp4.1LG04g09200.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023530026.1	0.0	95.88	calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita pepo subsp. pep...	[more]
XP_022930295.1	0.0	93.81	calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita moschata] >XP_0...	[more]
XP_023002661.1	4.43e-314	92.37	calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita maxima] >XP_023...	[more]
KAG7021759.1	4.57e-301	79.16	Calmodulin-binding receptor-like cytoplasmic kinase 3, partial [Cucurbita argyro...	[more]
XP_038879737.1	1.75e-260	75.33	calmodulin-binding receptor-like cytoplasmic kinase 3 [Benincasa hispida]	[more]

BLAST of Cp4.1LG04g09200.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EUR2	0.0	93.81	calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita moschata OX=3...	[more]
A0A6J1KLX6	2.15e-314	92.37	calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita maxima OX=366...	[more]
A0A6J1H8N3	8.87e-256	73.23	calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita moschata OX=3...	[more]
A0A6J1C0H6	3.60e-255	72.66	calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Momordica charantia OX=...	[more]
A0A6J1JK63	5.11e-255	73.04	calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita maxima OX=366...	[more]

BLAST of Cp4.1LG04g09200.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G11520.1	1.5e-126	59.06	calmodulin-binding receptor-like cytoplasmic kinase 3	[more]
AT4G00330.1	1.6e-80	50.51	calmodulin-binding receptor-like cytoplasmic kinase 2	[more]
AT5G58940.1	2.1e-80	48.38	calmodulin-binding receptor-like cytoplasmic kinase 1	[more]
AT2G39360.1	1.0e-53	40.20	Protein kinase superfamily protein	[more]
AT5G39000.1	1.5e-52	39.02	Malectin/receptor-like protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 197..471 e-value: 2.7E-19 score: 80.1
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 197..471 score: 32.244614
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PFAM	PF07714	PK_Tyr_Ser-Thr	coord: 207..469 e-value: 9.0E-45 score: 153.0
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 274..483 e-value: 9.1E-58 score: 197.0
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 172..273 e-value: 1.2E-12 score: 49.5
None	No IPR available	PIRSR	PIRSR037993-2	PIRSR037993-2	coord: 314..402 e-value: 1.7E-8 score: 31.6
None	No IPR available	PIRSR	PIRSR037921-1	PIRSR037921-1	coord: 230..402 e-value: 2.2E-24 score: 84.3
None	No IPR available	PIRSR	PIRSR000605-51	PIRSR000605-51	coord: 186..400 e-value: 1.4E-12 score: 45.0
None	No IPR available	PIRSR	PIRSR037014-2	PIRSR037014-2	coord: 241..400 e-value: 1.8E-14 score: 50.8
None	No IPR available	PANTHER	PTHR27003:SF274	NODULATION RECEPTOR KINASE-LIKE PROTEIN	coord: 58..477
None	No IPR available	PANTHER	PTHR27003	OS07G0166700 PROTEIN	coord: 58..477
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 318..330
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 180..472

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG04g09200	Cp4.1LG04g09200	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG04g09200.1:exon:001	Cp4.1LG04g09200.1:exon:001	exon
Cp4.1LG04g09200.1:exon:002	Cp4.1LG04g09200.1:exon:002	exon
Cp4.1LG04g09200.1:exon:003	Cp4.1LG04g09200.1:exon:003	exon
Cp4.1LG04g09200.1:exon:004	Cp4.1LG04g09200.1:exon:004	exon
Cp4.1LG04g09200.1:exon:005	Cp4.1LG04g09200.1:exon:005	exon
Cp4.1LG04g09200.1:exon:006	Cp4.1LG04g09200.1:exon:006	exon
Cp4.1LG04g09200.1:exon:007	Cp4.1LG04g09200.1:exon:007	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG04g09200.1:five_prime_utr:002	Cp4.1LG04g09200.1:five_prime_utr:002	five_prime_UTR
Cp4.1LG04g09200.1:five_prime_utr:001	Cp4.1LG04g09200.1:five_prime_utr:001	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG04g09200.1:cds:006	Cp4.1LG04g09200.1:cds:006	CDS
Cp4.1LG04g09200.1:cds:005	Cp4.1LG04g09200.1:cds:005	CDS
Cp4.1LG04g09200.1:cds:004	Cp4.1LG04g09200.1:cds:004	CDS
Cp4.1LG04g09200.1:cds:003	Cp4.1LG04g09200.1:cds:003	CDS
Cp4.1LG04g09200.1:cds:002	Cp4.1LG04g09200.1:cds:002	CDS
Cp4.1LG04g09200.1:cds:001	Cp4.1LG04g09200.1:cds:001	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG04g09200.1:three_prime_utr:001	Cp4.1LG04g09200.1:three_prime_utr:001	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG04g09200.1	Cp4.1LG04g09200.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0046777	protein autophosphorylation
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity