Cp4.1LG02g01900.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g01900.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	E3 SUMO-protein ligase MMS21
Location	Cp4.1LG02: 4314686 .. 4317511 (-)
Sequence length	1032
RNA-Seq Expression	Cp4.1LG02g01900.1
Synteny	Cp4.1LG02g01900.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTTTTCTAAGCGGGTATTAGCTTTTGGCGCTTAAACCAAACCATCGCCGGAGGGGGTTTCACTTTCGGTTATCTCTTCTTCAAAGGAATGGCGTCCACTTCCAATTCCAATTCAACTGGCGTTTCCGAAAGAATCAAATCCGCTGCCACTATTATGAACTCTGAGAATCAATCTCTCCTTGCCGTAACTTTCTTAATACTACTCTATTTCTTCTTTTTATGCTTTCAATTTGTTGTTCTTTTTGTTCTACTGTTTGTGCTTTTCCGATCGACAGATTAAATTTTCTAATTTCCATGGAAAATGAAGTTGCCGTTGCTAAATACATCTTGTTTTTAGATTTAAGATCTCGATTTCCTATATAGAGCGTCAGTTCTAACGCTGTATTTCCTTCACTATGCTCTGTGAACTGTGTTTTCTTGATTTCTGAGAACTGAAAAACTATTGGAAGATGATCTTGTGTATTCTTTTAATGGAAAATCCCCCCGCCCAACCCGTTTTGTTCAGTTCGTGGTCCGAGAAGACGAGATAAGAATGGTGAATTAAATTTGAGGAGTGATTGGGATTATTAGGTTGAAAAGTATGATGATGGGTTATCTGTTTATTTCTGTGATTGGATTTTTCTGTCTTAATTTCAGGAATTACGGAAGTCGCTGATCATGATGAAGGAAATCGGGGTGGATTTGGAGAGGGACAATCAGTCGAGGATGGTACTATGAACTATTGACCTTAATTTCGAATATTCAGAAATTTTTGTAATTTGTTACTTTGGAAGTGAATGTTGTTAGGATAAGATATTGTTTGTAATAAAATATACTAAGAAGCAAGTACCTAACATGTTCATTGCTCATTGTTGATCATTGTTGCTTGGAACCCTTAACTAATGATTTACTTGGTATTTGAACATTATTTTAGGTCAAGGAGCTTGAAAACTCTGTTGTTGAGCTGTTGGGTACTTATGAAAAATGTAGCAACTTTTCATCTGCAATTCAGTCAGTTGGAAATATATATGAACCACAAGAAGAGGTGCTTGAATGCATTTTCCACTACACTCTTTTTTTTGGTTAATTTTCATTGTGATGGTTCCTTCCTTCGTACATAATATTCTATGTTGATCATGTAGTTAACTGATTTTGAGAAACTACTTGACAATGAAGTTGCAAAAGTCAGCGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGACAGTTTAGAGAAGCTATTTGGGTATGCATCTTCATCTTCTTTAATATGCACTAGGAAATTTTGGAATGCGTTAGTTCACTTCTATGACTTTCTACTATTCGGGCTTTAGAACTGATTATGCGTGGCAGCAACCCTCAAATCAGTGCAAATAATTATACTAATACTGTGAAAATTCCCAACATATGAGAAGTAAGCAAGAGAAAATTCATAAGTGATTGGAAACCATTTTTTTGTTCTTCCAATTGCATTATTCTTTCCAGTGCTTTAACTTATCTCCCAATATTCCGGCAGAATGTTCATCACGCAGGACAACCAATGCCTGGTGAGGAGCAGGAGGACGTTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCGTTGAGTGGAAAGCCTGTCACAGAATTGGCAGAACCTGTTCGAAGGTATTTTACAACTAAACATATTTTCATGAAGGATAAAGAATTGGAACCATTTCTGTTAGCATTGAAAAAGAAGTCTGCTTTGTTAGAATTGTTGGGAGGGAGTCGTACGTTGGCTAATTTAGGAAATGATCGTGGTTTATAAGTAAAGAATACATCTCCATTGGTACAAGGCCATTTGAAAAAGCCCAAAGCAAAGTCATGAGAGCTTATGCTCAAAGTAGACAATATCGTACCATTGTGGAGAGTCGTTATTCCTAACATGTTTGTCTATTCACTGTGTACTAACAGCATGGATGTTGAAAGGGAATGGCTACATGCTTTATTTTCTCTAGTTTCATTTTTCAAGTGGATGCCTAAAAAATAGAATGAGCTTGGATCTTCTACTTTTTCAGAGCCGAGTTTCGAAATGGTTTTTTGTTCCTTAAAACAAATAACCATTTTAGGTTGTGGAAAAGATTCACTGACATGGAATGTATAGGTCTTCTTGTTTTTTCTTGGTTTTTTTATTTTGAGTTCAACCACACGTTGAAGAGGACGATTCTAATTTTTTATTTTTCAATCGATGATATAATGCTTATGTTTAAGTTAACTTCCATAGCCTGTTGTTATAGATCAGTACGGCCAGGCCATTGTGCATTTAAACACTTCTACTAAGTCTCTTTTTTGTCTCTACTTTCTGCAATTTTCAGTACGGAATGCAAGCACGTCTACGAAAAGGTTGCCATAATGCAGTACCTAAAATCCAAGAGATCACGAGCTCAATGCCCTGTTGCTGGTATTTGAATCTACTACATTACATGTACGACATAGAATGTTTTTAGATTGCTCGTCTCACTTGACCTCTTTGTTCTTATAGCCTGTCCCAAGATGTTGCAGCCTGAAAAGGTCATTTCTGATCCATTCTTACCGATTGAAATTGATGAACTACGCAAAACGTCTAAGCATTCAGTGAGGATACAGGATTTCACAGAGGTTGATGCAGATTAACACCACTGAAGTAACTTCTTTTACATATTTTGTCATTGTCTTAGTAGATTTCTTGGATCAAATGTTCTGCTTGGTAACTTTAGCTCGTTAGGGATGTTCATGGTTATCTATATCTATCAATATGAATTGTCTCTATTTATGTGCGAATGGAGAATTGTGGCTTCATTCTCAAATAGCCATTGCCCCTTAAATTTT

mRNA sequence

TTTTTTTTCTAAGCGGGTATTAGCTTTTGGCGCTTAAACCAAACCATCGCCGGAGGGGGTTTCACTTTCGGTTATCTCTTCTTCAAAGGAATGGCGTCCACTTCCAATTCCAATTCAACTGGCGTTTCCGAAAGAATCAAATCCGCTGCCACTATTATGAACTCTGAGAATCAATCTCTCCTTGCCGAATTACGGAAGTCGCTGATCATGATGAAGGAAATCGGGGTGGATTTGGAGAGGGACAATCAGTCGAGGATGGTCAAGGAGCTTGAAAACTCTGTTGTTGAGCTGTTGGGTACTTATGAAAAATGTAGCAACTTTTCATCTGCAATTCAGTCAGTTGGAAATATATATGAACCACAAGAAGAGTTAACTGATTTTGAGAAACTACTTGACAATGAAGTTGCAAAAGTCAGCGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGACAGTTTAGAGAAGCTATTTGGAATGTTCATCACGCAGGACAACCAATGCCTGGTGAGGAGCAGGAGGACGTTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCGTTGAGTGGAAAGCCTGTCACAGAATTGGCAGAACCTGTTCGAAGTACGGAATGCAAGCACGTCTACGAAAAGGTTGCCATAATGCAGTACCTAAAATCCAAGAGATCACGAGCTCAATGCCCTGTTGCTGCCTGTCCCAAGATGTTGCAGCCTGAAAAGGTCATTTCTGATCCATTCTTACCGATTGAAATTGATGAACTACGCAAAACGTCTAAGCATTCAGTGAGGATACAGGATTTCACAGAGGTTGATGCAGATTAACACCACTGAAGTAACTTCTTTTACATATTTTGTCATTGTCTTAGTAGATTTCTTGGATCAAATGTTCTGCTTGGTAACTTTAGCTCGTTAGGGATGTTCATGGTTATCTATATCTATCAATATGAATTGTCTCTATTTATGTGCGAATGGAGAATTGTGGCTTCATTCTCAAATAGCCATTGCCCCTTAAATTTT

Coding sequence (CDS)

ATGGCGTCCACTTCCAATTCCAATTCAACTGGCGTTTCCGAAAGAATCAAATCCGCTGCCACTATTATGAACTCTGAGAATCAATCTCTCCTTGCCGAATTACGGAAGTCGCTGATCATGATGAAGGAAATCGGGGTGGATTTGGAGAGGGACAATCAGTCGAGGATGGTCAAGGAGCTTGAAAACTCTGTTGTTGAGCTGTTGGGTACTTATGAAAAATGTAGCAACTTTTCATCTGCAATTCAGTCAGTTGGAAATATATATGAACCACAAGAAGAGTTAACTGATTTTGAGAAACTACTTGACAATGAAGTTGCAAAAGTCAGCGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGACAGTTTAGAGAAGCTATTTGGAATGTTCATCACGCAGGACAACCAATGCCTGGTGAGGAGCAGGAGGACGTTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCGTTGAGTGGAAAGCCTGTCACAGAATTGGCAGAACCTGTTCGAAGTACGGAATGCAAGCACGTCTACGAAAAGGTTGCCATAATGCAGTACCTAAAATCCAAGAGATCACGAGCTCAATGCCCTGTTGCTGCCTGTCCCAAGATGTTGCAGCCTGAAAAGGTCATTTCTGATCCATTCTTACCGATTGAAATTGATGAACTACGCAAAACGTCTAAGCATTCAGTGAGGATACAGGATTTCACAGAGGTTGATGCAGATTAA

Protein sequence

MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKELENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQDFTEVDAD

Homology

[Close]

BLAST of Cp4.1LG02g01900.1 vs. ExPASy Swiss-Prot
Match: Q8GYH7 (E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.1e-69
Identity = 141/248 (56.85%), Postives = 184/248 (74.19%), Query Frame = 0


Query: 5   SNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKELENSV 64
           S S+S GV+ RI++A+ ++ S+N S LA++RK++ MMK I V LE++NQ+  VK+LENSV
Sbjct: 3   SASSSDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDLENSV 62

Query: 65  VELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHSIIRQ 124
            ELL  +  C++ S+AIQSV N Y+P E+LTDF+KLLD+E  K+    SS  QN  ++RQ
Sbjct: 63  AELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDHLMRQ 122

Query: 125 FREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTECKHVY 184
           FREA+WNVHHAG+PMPG++ ED+VMTSTQC LLN+TCPLSGKPVTELA+PVRS +C+HVY
Sbjct: 123 FREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDCRHVY 182

Query: 185 EKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR---IQD 244
           EK  I+ Y+ +    A CPVA C   LQ  KVI D  L  EI+E+R  +K S R   I+D
Sbjct: 183 EKSVILHYIVN-NPNANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAEVIED 242

Query: 245 FTE-VDAD 249
           FTE VD D
Sbjct: 243 FTEDVDED 249

[Close]

BLAST of Cp4.1LG02g01900.1 vs. NCBI nr
Match: XP_023525588.1 (E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-protein ligase MMS21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 476 bits (1225), Expect = 4.75e-169
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60
           MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120
           ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180
           IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240
           KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTEVDAD 248
           DFTEVDAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Cp4.1LG02g01900.1 vs. NCBI nr
Match: KAG6607898.1 (E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 474 bits (1220), Expect = 2.75e-168
Identity = 247/248 (99.60%), Postives = 247/248 (99.60%), Query Frame = 0


Query: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60
           MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120
           ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNL NHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLHNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180
           IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240
           KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTEVDAD 248
           DFTEVDAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Cp4.1LG02g01900.1 vs. NCBI nr
Match: XP_022941288.1 (E3 SUMO-protein ligase MMS21 [Cucurbita moschata])

HSP 1 Score: 473 bits (1216), Expect = 1.12e-167
Identity = 246/248 (99.19%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60
           MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGV+LERDNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVELERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120
           ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLD+EVAKVSENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180
           IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240
           KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTEVDAD 248
           DFTEVDAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Cp4.1LG02g01900.1 vs. NCBI nr
Match: XP_022981951.1 (E3 SUMO-protein ligase MMS21 [Cucurbita maxima])

HSP 1 Score: 469 bits (1206), Expect = 4.02e-166
Identity = 247/250 (98.80%), Postives = 247/250 (98.80%), Query Frame = 0


Query: 1   MASTSNSNS--TGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK 60
           MASTSNSNS  TGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK
Sbjct: 1   MASTSNSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK 60

Query: 61  ELENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN 120
           ELENSVVELL TYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN
Sbjct: 61  ELENSVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN 120

Query: 121 HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST 180
           HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST
Sbjct: 121 HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST 180

Query: 181 ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR 240
           ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR
Sbjct: 181 ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR 240

Query: 241 IQDFTEVDAD 248
           IQDFTEVDAD
Sbjct: 241 IQDFTEVDAD 250

[Close]

BLAST of Cp4.1LG02g01900.1 vs. NCBI nr
Match: XP_022139610.1 (E3 SUMO-protein ligase MMS21 isoform X1 [Momordica charantia])

HSP 1 Score: 420 bits (1079), Expect = 8.47e-147
Identity = 215/248 (86.69%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60
           MASTS++ S+ VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG+DLERDNQS MVK+L
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60

Query: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120
           EN+VVELL TYE C NFSSAIQSVGNIYEP+EELTDFEKLLD+EVAKVSENSSSNLQNHS
Sbjct: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180
           IIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180

Query: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240
           KHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVRIQ
Sbjct: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240

Query: 241 DFTEVDAD 248
           DFTE+DA+
Sbjct: 241 DFTELDAE 248

[Close]

BLAST of Cp4.1LG02g01900.1 vs. ExPASy TrEMBL
Match: A0A6J1FRP6 (E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 SV=1)

HSP 1 Score: 473 bits (1216), Expect = 5.42e-168
Identity = 246/248 (99.19%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60
           MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGV+LERDNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVELERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120
           ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLD+EVAKVSENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180
           IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240
           KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTEVDAD 248
           DFTEVDAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Cp4.1LG02g01900.1 vs. ExPASy TrEMBL
Match: A0A6J1IXZ2 (E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV=1)

HSP 1 Score: 469 bits (1206), Expect = 1.95e-166
Identity = 247/250 (98.80%), Postives = 247/250 (98.80%), Query Frame = 0


Query: 1   MASTSNSNS--TGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK 60
           MASTSNSNS  TGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK
Sbjct: 1   MASTSNSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK 60

Query: 61  ELENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN 120
           ELENSVVELL TYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN
Sbjct: 61  ELENSVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN 120

Query: 121 HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST 180
           HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST
Sbjct: 121 HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST 180

Query: 181 ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR 240
           ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR
Sbjct: 181 ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR 240

Query: 241 IQDFTEVDAD 248
           IQDFTEVDAD
Sbjct: 241 IQDFTEVDAD 250

[Close]

BLAST of Cp4.1LG02g01900.1 vs. ExPASy TrEMBL
Match: A0A6J1CCS6 (E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010470 PE=3 SV=1)

HSP 1 Score: 420 bits (1079), Expect = 4.10e-147
Identity = 215/248 (86.69%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60
           MASTS++ S+ VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG+DLERDNQS MVK+L
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60

Query: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120
           EN+VVELL TYE C NFSSAIQSVGNIYEP+EELTDFEKLLD+EVAKVSENSSSNLQNHS
Sbjct: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180
           IIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180

Query: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240
           KHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVRIQ
Sbjct: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240

Query: 241 DFTEVDAD 248
           DFTE+DA+
Sbjct: 241 DFTELDAE 248

[Close]

BLAST of Cp4.1LG02g01900.1 vs. ExPASy TrEMBL
Match: A0A5D3DWX9 (E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001530 PE=3 SV=1)

HSP 1 Score: 409 bits (1052), Expect = 5.32e-143
Identity = 210/248 (84.68%), Postives = 228/248 (91.94%), Query Frame = 0


Query: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60
           MAS S+S STGV+ RIKSAATIM+SENQSLLAELRK+LIMMKEIGVDLE++NQ++MVKEL
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLLAELRKTLIMMKEIGVDLEKENQNKMVKEL 60

Query: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120
           E S+VELL  YE C+NFSSAIQSVGN YEP+EELTDFEKLLD+EVAKVS NSSSN  NH 
Sbjct: 61  EKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSVNSSSNFANHP 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180
           IIRQFREAIWNVHHAGQPM GEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSAEC 180

Query: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240
           KH+YEK AIMQYL SK+SRAQCPVAACPKMLQP+KV+ DPFL IEIDELRK S+HS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSERIQ 240

Query: 241 DFTEVDAD 248
           DFTE+DAD
Sbjct: 241 DFTELDAD 248

[Close]

BLAST of Cp4.1LG02g01900.1 vs. ExPASy TrEMBL
Match: A0A1S3CLF9 (E3 SUMO-protein ligase MMS21 OS=Cucumis melo OX=3656 GN=LOC103501788 PE=3 SV=1)

HSP 1 Score: 409 bits (1052), Expect = 5.32e-143
Identity = 210/248 (84.68%), Postives = 228/248 (91.94%), Query Frame = 0


Query: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60
           MAS S+S STGV+ RIKSAATIM+SENQSLLAELRK+LIMMKEIGVDLE++NQ++MVKEL
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLLAELRKTLIMMKEIGVDLEKENQNKMVKEL 60

Query: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120
           E S+VELL  YE C+NFSSAIQSVGN YEP+EELTDFEKLLD+EVAKVS NSSSN  NH 
Sbjct: 61  EKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSVNSSSNFANHP 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180
           IIRQFREAIWNVHHAGQPM GEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSAEC 180

Query: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240
           KH+YEK AIMQYL SK+SRAQCPVAACPKMLQP+KV+ DPFL IEIDELRK S+HS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSERIQ 240

Query: 241 DFTEVDAD 248
           DFTE+DAD
Sbjct: 241 DFTELDAD 248

[Close]

BLAST of Cp4.1LG02g01900.1 vs. TAIR 10
Match: AT3G15150.1 (RING/U-box superfamily protein )

HSP 1 Score: 264.6 bits (675), Expect = 7.9e-71
Identity = 141/248 (56.85%), Postives = 184/248 (74.19%), Query Frame = 0


Query: 5   SNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKELENSV 64
           S S+S GV+ RI++A+ ++ S+N S LA++RK++ MMK I V LE++NQ+  VK+LENSV
Sbjct: 3   SASSSDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDLENSV 62

Query: 65  VELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHSIIRQ 124
            ELL  +  C++ S+AIQSV N Y+P E+LTDF+KLLD+E  K+    SS  QN  ++RQ
Sbjct: 63  AELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDHLMRQ 122

Query: 125 FREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTECKHVY 184
           FREA+WNVHHAG+PMPG++ ED+VMTSTQC LLN+TCPLSGKPVTELA+PVRS +C+HVY
Sbjct: 123 FREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDCRHVY 182

Query: 185 EKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR---IQD 244
           EK  I+ Y+ +    A CPVA C   LQ  KVI D  L  EI+E+R  +K S R   I+D
Sbjct: 183 EKSVILHYIVN-NPNANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAEVIED 242

Query: 245 FTE-VDAD 249
           FTE VD D
Sbjct: 243 FTEDVDED 249

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g01900.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYH7	1.1e-69	56.85	E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1	[more]

BLAST of Cp4.1LG02g01900.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023525588.1	4.75e-169	100.00	E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-...	[more]
KAG6607898.1	2.75e-168	99.60	E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022941288.1	1.12e-167	99.19	E3 SUMO-protein ligase MMS21 [Cucurbita moschata]	[more]
XP_022981951.1	4.02e-166	98.80	E3 SUMO-protein ligase MMS21 [Cucurbita maxima]	[more]
XP_022139610.1	8.47e-147	86.69	E3 SUMO-protein ligase MMS21 isoform X1 [Momordica charantia]	[more]

BLAST of Cp4.1LG02g01900.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FRP6	5.42e-168	99.19	E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 ...	[more]
A0A6J1IXZ2	1.95e-166	98.80	E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV...	[more]
A0A6J1CCS6	4.10e-147	86.69	E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5D3DWX9	5.32e-143	84.68	E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3CLF9	5.32e-143	84.68	E3 SUMO-protein ligase MMS21 OS=Cucumis melo OX=3656 GN=LOC103501788 PE=3 SV=1	[more]

BLAST of Cp4.1LG02g01900.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15150.1	7.9e-71	56.85	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 153..239 e-value: 5.0E-18 score: 67.1
IPR004181	Zinc finger, MIZ-type	PFAM	PF11789	zf-Nse	coord: 146..207 e-value: 2.1E-15 score: 56.4
IPR004181	Zinc finger, MIZ-type	CDD	cd16651	SPL-RING_NSE2	coord: 160..228 e-value: 8.80948E-23 score: 86.1444
IPR026846	E3 SUMO-protein ligase Nse2 (Mms21)	PANTHER	PTHR21330	UNCHARACTERIZED	coord: 6..236
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 159..231

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG02g01900	Cp4.1LG02g01900	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG02g01900.1:three_prime_utr:001	Cp4.1LG02g01900.1:three_prime_utr:001	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG02g01900.1:exon:007	Cp4.1LG02g01900.1:exon:007	exon
Cp4.1LG02g01900.1:exon:006	Cp4.1LG02g01900.1:exon:006	exon
Cp4.1LG02g01900.1:exon:005	Cp4.1LG02g01900.1:exon:005	exon
Cp4.1LG02g01900.1:exon:004	Cp4.1LG02g01900.1:exon:004	exon
Cp4.1LG02g01900.1:exon:003	Cp4.1LG02g01900.1:exon:003	exon
Cp4.1LG02g01900.1:exon:002	Cp4.1LG02g01900.1:exon:002	exon
Cp4.1LG02g01900.1:exon:001	Cp4.1LG02g01900.1:exon:001	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG02g01900.1:cds:007	Cp4.1LG02g01900.1:cds:007	CDS
Cp4.1LG02g01900.1:cds:006	Cp4.1LG02g01900.1:cds:006	CDS
Cp4.1LG02g01900.1:cds:005	Cp4.1LG02g01900.1:cds:005	CDS
Cp4.1LG02g01900.1:cds:004	Cp4.1LG02g01900.1:cds:004	CDS
Cp4.1LG02g01900.1:cds:003	Cp4.1LG02g01900.1:cds:003	CDS
Cp4.1LG02g01900.1:cds:002	Cp4.1LG02g01900.1:cds:002	CDS
Cp4.1LG02g01900.1:cds:001	Cp4.1LG02g01900.1:cds:001	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG02g01900.1:five_prime_utr:001	Cp4.1LG02g01900.1:five_prime_utr:001	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG02g01900.1	Cp4.1LG02g01900.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0060250	germ-line stem-cell niche homeostasis
biological_process	GO:0032876	negative regulation of DNA endoreduplication
biological_process	GO:0008284	positive regulation of cell population proliferation
biological_process	GO:0080038	positive regulation of cytokinin-activated signaling pathway
biological_process	GO:0045931	positive regulation of mitotic cell cycle
biological_process	GO:0016925	protein sumoylation
biological_process	GO:0048509	regulation of meristem development
biological_process	GO:0010082	regulation of root meristem growth
cellular_component	GO:0030915	Smc5-Smc6 complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0019789	SUMO transferase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0061665	SUMO ligase activity