Cp4.1LG01g03680.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g03680.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Cp4.1LG01: 1646236 .. 1647202 (-)
Sequence length	606
RNA-Seq Expression	Cp4.1LG01g03680.1
Synteny	Cp4.1LG01g03680.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTCTTCTTCGTTTCAATTACGTTCACCACCTCGGTCGTGGCTTCTTCTTCATCCGTTACCCGTCCCGTGAAACCATACATCAAAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCCCTCACCTTACATGCTTCACAAACCAAAAGAACACAACAAGAGTTCTGCAGAGCAACCATGGCTTCTTCTCTCAAAACTACACGAGATGCCACCTCCATAATCTCCAAGTTATCTCGCCAGAAGATGTCTACCTATGAAGCTAAGGTCATTAGAGATTGTGTTGATAATCTTCAAGACTCTACCGACGAGCTCCAAAGAGCCATCGCTGCAATAAAAAACTTGAGTCGTGCTAAAGATGTGAACTTTCAGGTTATTTAAACTTCTACTTAAACCCAAAAGGTTTAAATTTATATGTTTTCGTAAATTTTATCTCAAATTCATATTTTAAACGTCATTCTACATCATGTATTTGAAATAAAATTTTAAACCTAAAATCATCCAAAATATATATATATATATCTTTAGACATGATCTTAGCACACTTATGGTTTGACCATAAGGTTTTAAATACGAAAGTCAACCTAGAGACGTGAAAAATATATATTTAATCAAATAAGTTAACATTTTTTATAAATGAAATTAGGTTGATTATGAATTTTAATTAAACCCTTTAAATAAGTGAAGGTAGATTGGGTTAAATTTAATATTTGTATTCATATTGTGAACACAGTTACATAGTATCAAAACATGGATGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAAGAAGAGTGTTGCGGTTGTGGTGAGGCATATCAGTAACGCCTTGTCCCTCCTCAACACCTTCAAATACAAATCCTGA

mRNA sequence

ATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTCTTCTTCGTTTCAATTACGTTCACCACCTCGGTCGTGGCTTCTTCTTCATCCGTTACCCGTCCCGTGAAACCATACATCAAAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCCCTCACCTTACATGCTTCACAAACCAAAAGAACACAACAAGAGTTCTGCAGAGCAACCATGGCTTCTTCTCTCAAAACTACACGAGATGCCACCTCCATAATCTCCAAGTTATCTCGCCAGAAGATGTCTACCTATGAAGCTAAGGTCATTAGAGATTGTGTTGATAATCTTCAAGACTCTACCGACGAGCTCCAAAGAGCCATCGCTGCAATAAAAAACTTGAGTCGTGCTAAAGATGTGAACTTTCAGTTACATAGTATCAAAACATGGATGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAAGAAGAGTGTTGCGGTTGTGGTGAGGCATATCAGTAACGCCTTGTCCCTCCTCAACACCTTCAAATACAAATCCTGA

Coding sequence (CDS)

ATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTCTTCTTCGTTTCAATTACGTTCACCACCTCGGTCGTGGCTTCTTCTTCATCCGTTACCCGTCCCGTGAAACCATACATCAAAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCCCTCACCTTACATGCTTCACAAACCAAAAGAACACAACAAGAGTTCTGCAGAGCAACCATGGCTTCTTCTCTCAAAACTACACGAGATGCCACCTCCATAATCTCCAAGTTATCTCGCCAGAAGATGTCTACCTATGAAGCTAAGGTCATTAGAGATTGTGTTGATAATCTTCAAGACTCTACCGACGAGCTCCAAAGAGCCATCGCTGCAATAAAAAACTTGAGTCGTGCTAAAGATGTGAACTTTCAGTTACATAGTATCAAAACATGGATGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAAGAAGAGTGTTGCGGTTGTGGTGAGGCATATCAGTAACGCCTTGTCCCTCCTCAACACCTTCAAATACAAATCCTGA

Protein sequence

MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQRAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSLLNTFKYKS

Homology

[Close]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.4e-22
Identity = 64/187 (34.22%), Postives = 106/187 (56.68%), Query Frame = 0


Query: 17  SITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQEFCRATMA 76
           ++ F+  +V  +++   P   +IK +C  T YP +CE SL+ +A   +   QE     + 
Sbjct: 7   TLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQ 66

Query: 77  SSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLQDSTDELQRAIAAIKNLSRAK-- 136
            SL  T+ A + + +L++ K +   +   I DC++ ++DS D + R+   +KNLS AK  
Sbjct: 67  VSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGN 126

Query: 137 DVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSL 196
           D  F++ +++TW+SAA TD  TC DG    AG+ +  K+K+ V+  V  V R  SNAL+L
Sbjct: 127 DFTFRMSNVETWVSAALTDETTCMDGF---AGKGMDGKIKESVRAQVVAVARVTSNALAL 186

Query: 197 LNTFKYK 201
           +N F  K
Sbjct: 187 VNNFAAK 190

[Close]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy Swiss-Prot
Match: Q9SB38 (Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 9.5e-19
Identity = 58/191 (30.37%), Postives = 111/191 (58.12%), Query Frame = 0


Query: 10  LTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQE 69
           LTL  F++ +      A+     +    Y+K AC +T YP +C   L+ ++S  K    +
Sbjct: 9   LTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKSDPIK 68

Query: 70  FCRATMASSLKTTRDATSIISKLSRQKMS--TYEAKVIRDCVDNLQDSTDELQRAIAAIK 129
            C  ++  ++K+ ++AT ++S L ++  +  ++E  +++DCVD ++D+ DEL++A+A +K
Sbjct: 69  LCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVAEMK 128

Query: 130 NL-SRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRH 189
            +    K     L ++KTW+S+A TD  TC+DG   G   +V  + K++VKK+++ + + 
Sbjct: 129 YVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKKAISELSKT 188

Query: 190 ISNALSLLNTF 198
            SN L+LL  +
Sbjct: 189 TSNTLALLTHY 196

[Close]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-14
Identity = 60/200 (30.00%), Postives = 103/200 (51.50%), Query Frame = 0


Query: 11  TLFFFVSITFTTSVVASSSSVTRPVKP------YIKKACKTTPYPRLCEISLTLHASQTK 70
           TLF F+    +T+++ +SSS  R          +I+ +CK T YP +C  SLT +A+  +
Sbjct: 8   TLFLFL---LSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQ 67

Query: 71  RTQQEFCRATMASSLKTTRDATSIISKLSR-QKMSTYEAKVIRDCVDNLQDSTDELQRAI 130
            + +      +  ++   +     + +L R   +   E + ++DC++ + D+ D L  +I
Sbjct: 68  TSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSI 127

Query: 131 AAIKNLSRAKDVN---FQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 190
             +K    AK  +   F + + +TW SAA T+A TCSDG    AGR +  +VK  V+  +
Sbjct: 128 HEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGF---AGRVMDGRVKNSVRARI 187

Query: 191 AVVVRHISNALSLLNTFKYK 201
             + R  SNAL+L+N F  K
Sbjct: 188 LNLGRGTSNALALINAFAKK 201

[Close]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.4e-14
Identity = 61/201 (30.35%), Postives = 108/201 (53.73%), Query Frame = 0


Query: 6   LKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTK- 65
           LK+ + L   +SIT   S  +S++        +I  +C+TT YP LC  +L+ +A++ + 
Sbjct: 3   LKNTIFLVILLSITILQS--SSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRH 62

Query: 66  RTQQEFCRATMASSLKTTRDATSIISKLSRQ--KMSTYEAKVIRDCVDNLQDSTDELQRA 125
              Q+  +  +  SL   +  T  ++KL+++  K    E   I+DC++ L +S D L ++
Sbjct: 63  NNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQS 122

Query: 126 IAAIKNLSRA---KDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKS 185
           +  +     A   +D  +++ +++TW+SAA TD  TC DG S    R +  KVK+ ++  
Sbjct: 123 VKELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFS---ERAMGGKVKRLIRYK 182

Query: 186 VAVVVRHISNALSLLNTFKYK 201
           V  V +  SNAL+L+N F  K
Sbjct: 183 VVHVAQVTSNALALVNQFAEK 198

[Close]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.6e-12
Identity = 55/182 (30.22%), Postives = 94/182 (51.65%), Query Frame = 0


Query: 22  TSVVASSSSVT---RPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQEFCRATMASS 81
           +S+  SSSS T   +    YIK +C  T Y  +C  SL+ +AS  +   Q+     +  +
Sbjct: 128 SSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLT 187

Query: 82  LKTTRDATSIISKLSR-QKMSTYEAKVIRDCVDNLQDSTDELQRAIAAIKNLSRAKDVNF 141
           L + + A+  +  +S    ++  E   + DCV+ + DS   LQ +I  + +++      F
Sbjct: 188 LSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKF 247

Query: 142 Q--LHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSLLN 198
           +  +  ++TW+SAA T+  TC DG S      V++ VK  V++ V  V R  SNAL+L+N
Sbjct: 248 EMVMSDVETWVSAALTNDDTCMDGFS-----LVKTAVKDLVRRHVVEVARLTSNALALIN 304

[Close]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr
Match: XP_023542401.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 378 bits (970), Expect = 9.73e-132
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA 60
           MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA
Sbjct: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA 60

Query: 61  SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQ 120
           SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQ
Sbjct: 61  SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQ 120

Query: 121 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180
           RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV
Sbjct: 121 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180

Query: 181 AVVVRHISNALSLLNTFKYKS 201
           AVVVRHISNALSLLNTFKYKS
Sbjct: 181 AVVVRHISNALSLLNTFKYKS 201

[Close]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr
Match: KAG7030832.1 (hypothetical protein SDJN02_04869, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364 bits (935), Expect = 2.11e-126
Identity = 193/201 (96.02%), Postives = 198/201 (98.51%), Query Frame = 0


Query: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA 60
           MEFQILKSLLTLFFFVSIT TTSVVASSSS+TRPVKPYI KACKTTPYPRLCEISLTLHA
Sbjct: 1   MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHA 60

Query: 61  SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQ 120
           SQTK+TQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNL+DSTDEL+
Sbjct: 61  SQTKKTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELR 120

Query: 121 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180
           RAIAAIK+LSRAKD NFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV
Sbjct: 121 RAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180

Query: 181 AVVVRHISNALSLLNTFKYKS 201
           AVVVRHISNALSLLNTFKYKS
Sbjct: 181 AVVVRHISNALSLLNTFKYKS 201

[Close]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr
Match: XP_022942851.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 362 bits (930), Expect = 1.22e-125
Identity = 192/201 (95.52%), Postives = 197/201 (98.01%), Query Frame = 0


Query: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA 60
           MEFQILKSLLTLFFFVSIT TTSVVASSSS+TRPVKPYI KACKTTPYPRLCEISLTLHA
Sbjct: 1   MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHA 60

Query: 61  SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQ 120
           SQTKRTQQEFCRATM SSLKTTRDATSIISKLSRQK+STYEAKVIRDCVDNL+DSTDEL+
Sbjct: 61  SQTKRTQQEFCRATMTSSLKTTRDATSIISKLSRQKISTYEAKVIRDCVDNLKDSTDELR 120

Query: 121 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180
           RAIAAIK+LSRAKD NFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV
Sbjct: 121 RAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180

Query: 181 AVVVRHISNALSLLNTFKYKS 201
           AVVVRHISNALSLLNTFKYKS
Sbjct: 181 AVVVRHISNALSLLNTFKYKS 201

[Close]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr
Match: XP_022987359.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 357 bits (916), Expect = 1.66e-123
Identity = 188/201 (93.53%), Postives = 194/201 (96.52%), Query Frame = 0


Query: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA 60
           MEFQILKSLLTLFFFVSITFTTSVVASSSS+ RPVKPYIKKACKTTPYPRLCEISLTLHA
Sbjct: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHA 60

Query: 61  SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQ 120
           SQTK+TQQEFCRA MASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNL+DSTDEL 
Sbjct: 61  SQTKKTQQEFCRAAMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELH 120

Query: 121 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180
           RAIA  K+LSRAKD NFQLHSIKTW+SAAETDAMTCSDGLSGGAGRKVRSKVKQEV+KSV
Sbjct: 121 RAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSV 180

Query: 181 AVVVRHISNALSLLNTFKYKS 201
           AVVVRHISNALSLL TFKYKS
Sbjct: 181 AVVVRHISNALSLLTTFKYKS 201

[Close]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr
Match: XP_038882390.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 273 bits (699), Expect = 1.94e-90
Identity = 143/201 (71.14%), Postives = 168/201 (83.58%), Query Frame = 0


Query: 1   MEFQILKSLLTLFFFVSIT-FTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLH 60
           ME QILKS LTLFFF+ +T FTTS  ASSSS  RPV+P+I+KACK TPYPRLCE +L+L+
Sbjct: 1   MESQILKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLY 60

Query: 61  ASQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDEL 120
           ASQTKR QQE CRA M SSLK  ++ATSIISK+SR+K+S YEA+VI DC+DNL DS DE+
Sbjct: 61  ASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDEI 120

Query: 121 QRAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKS 180
           +RA  AIK LSR+KDV+FQL SIKTWMSAAETD +TC+DGLSG AGRKVR+KVK+EVK  
Sbjct: 121 RRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNC 180

Query: 181 VAVVVRHISNALSLLNTFKYK 200
             +VVR ISNALSL+N F YK
Sbjct: 181 SVIVVRQISNALSLINNFNYK 201

[Close]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy TrEMBL
Match: A0A6J1FQ13 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111447758 PE=4 SV=1)

HSP 1 Score: 362 bits (930), Expect = 5.90e-126
Identity = 192/201 (95.52%), Postives = 197/201 (98.01%), Query Frame = 0


Query: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA 60
           MEFQILKSLLTLFFFVSIT TTSVVASSSS+TRPVKPYI KACKTTPYPRLCEISLTLHA
Sbjct: 1   MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHA 60

Query: 61  SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQ 120
           SQTKRTQQEFCRATM SSLKTTRDATSIISKLSRQK+STYEAKVIRDCVDNL+DSTDEL+
Sbjct: 61  SQTKRTQQEFCRATMTSSLKTTRDATSIISKLSRQKISTYEAKVIRDCVDNLKDSTDELR 120

Query: 121 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180
           RAIAAIK+LSRAKD NFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV
Sbjct: 121 RAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180

Query: 181 AVVVRHISNALSLLNTFKYKS 201
           AVVVRHISNALSLLNTFKYKS
Sbjct: 181 AVVVRHISNALSLLNTFKYKS 201

[Close]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy TrEMBL
Match: A0A6J1JIN9 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111484936 PE=4 SV=1)

HSP 1 Score: 357 bits (916), Expect = 8.04e-124
Identity = 188/201 (93.53%), Postives = 194/201 (96.52%), Query Frame = 0


Query: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA 60
           MEFQILKSLLTLFFFVSITFTTSVVASSSS+ RPVKPYIKKACKTTPYPRLCEISLTLHA
Sbjct: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHA 60

Query: 61  SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQ 120
           SQTK+TQQEFCRA MASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNL+DSTDEL 
Sbjct: 61  SQTKKTQQEFCRAAMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELH 120

Query: 121 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180
           RAIA  K+LSRAKD NFQLHSIKTW+SAAETDAMTCSDGLSGGAGRKVRSKVKQEV+KSV
Sbjct: 121 RAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSV 180

Query: 181 AVVVRHISNALSLLNTFKYKS 201
           AVVVRHISNALSLL TFKYKS
Sbjct: 181 AVVVRHISNALSLLTTFKYKS 201

[Close]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy TrEMBL
Match: A0A1S3AXZ9 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1)

HSP 1 Score: 261 bits (667), Expect = 6.93e-86
Identity = 142/202 (70.30%), Postives = 164/202 (81.19%), Query Frame = 0


Query: 1   MEFQILKSLLTLFFFVSI--TFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTL 60
           ME QILKS LTLFFF  I  TFT S VASSSS  RPVKP+I+KACK TPYPRLCE +L+L
Sbjct: 1   MESQILKSSLTLFFFFIILTTFTPSAVASSSSTVRPVKPHIRKACKPTPYPRLCETALSL 60

Query: 61  HASQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDE 120
           +ASQTKR QQE CRA M SSLK  ++ATSIISKLSR+KMS YE +VI DC+D+L+DS DE
Sbjct: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVDE 120

Query: 121 LQRAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKK 180
           L+RA  AIK+LSR+KDV+FQL+SIKTW SAA TD +TC+DGLSGG+G KV  KVK+EVK 
Sbjct: 121 LRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVKN 180

Query: 181 SVAVVVRHISNALSLLNTFKYK 200
               VVR ISNALSL+N F YK
Sbjct: 181 CSINVVRQISNALSLINNFNYK 202

[Close]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy TrEMBL
Match: A0A0A0L671 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 256 bits (654), Expect = 6.80e-84
Identity = 137/203 (67.49%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 1   MEFQILKSLLTL---FFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLT 60
           ME QILKS LTL   FF +  TFT S V+SSSS  RPV+P+I+KACK TPYPRLCE +L+
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LHASQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTD 120
           L+ASQTKR QQE CRA M SSLK  ++ATSIISKLSR+KMS YEA+VI DC+DNL+DS D
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 ELQRAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVK 180
           EL+RA  AIK+LSR+KDV+FQL+SIKTWMSAA+TD +TC+DGLSGG+G KV   +K++VK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 KSVAVVVRHISNALSLLNTFKYK 200
                VVR ISNAL L+N F YK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy TrEMBL
Match: A0A6J1J533 (pectinesterase inhibitor 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483499 PE=4 SV=1)

HSP 1 Score: 247 bits (630), Expect = 2.40e-80
Identity = 132/196 (67.35%), Postives = 160/196 (81.63%), Query Frame = 0


Query: 6   LKSLLTLFFFVSI-TFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTK 65
           +KSLL LFFF+ I TFT S VA+SSS  +PVKP+I+KAC+ TPYPRLCE +L+L++SQTK
Sbjct: 1   MKSLLILFFFILIATFTISAVAASSSAIQPVKPHIRKACRPTPYPRLCETALSLYSSQTK 60

Query: 66  RTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQRAIA 125
           RTQQE CRA + SSLK  ++ATSIISKLS +K+S YEA+VI DC DNL+DS  EL+RA  
Sbjct: 61  RTQQELCRAAVVSSLKAAQNATSIISKLSHRKVSPYEAEVIGDCTDNLKDSIYELRRATT 120

Query: 126 AIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVV 185
           AIK+L+RA DV FQL SIKTW+SAAET+ MTC+DGLSGGAG KVRS VK+EVKK    V+
Sbjct: 121 AIKSLNRADDVEFQLSSIKTWLSAAETNIMTCTDGLSGGAGWKVRSMVKKEVKKCSVNVI 180

Query: 186 RHISNALSLLNTFKYK 200
           R  SNALSL++  KYK
Sbjct: 181 RQTSNALSLISNLKYK 196

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.5e-22
Identity = 65/201 (32.34%), Postives = 111/201 (55.22%), Query Frame = 0


Query: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA 60
           M  Q L  LL++ + +S+  T +  AS +  ++    +I+ +CKTT YP LC  SL+++A
Sbjct: 1   MAKQYLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYA 60

Query: 61  SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLQDSTDEL 120
           +  + + +      +A +L   +     +S+L+R K +   E + I+DCV+ + D+ D L
Sbjct: 61  NDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRL 120

Query: 121 QRAIAAIKNLSRAKDVN---FQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEV 180
            +++  +K    AKD +   + + + +TW SAA TD  TCSDG S   GR +  ++K  V
Sbjct: 121 TKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFS---GRVMDGRIKNSV 180

Query: 181 KKSVAVVVRHISNALSLLNTF 198
           +  +  V    SNALSL+N F
Sbjct: 181 RARIMNVGHETSNALSLINAF 198

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TAIR 10
Match: AT5G51520.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 7.9e-21
Identity = 63/205 (30.73%), Postives = 112/205 (54.63%), Query Frame = 0


Query: 4   QILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQT 63
           Q+L SL  +F F + T       S+SS ++  K ++K AC +T YP  C  SL+ ++S  
Sbjct: 3   QVLYSLTIVFVFFASTNIQKTSGSASSYSQNHKTFVKTACNSTTYPDKCYKSLSSYSSNI 62

Query: 64  KRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTY---------EAKVIRDCVDNLQD 123
           K    + C   +  ++K+ ++ATS++SKL +    +          EA +++DC++ ++D
Sbjct: 63  KSDPIKLCTTALNLNVKSAKEATSVVSKLLKMSQKSTAGRKGKMLPEALILKDCLEEMKD 122

Query: 124 STDELQRAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQ 183
           +  EL++AI  +KNL     +   + +++TW+S+A TD  TC+DG       KV  + K+
Sbjct: 123 TIIELKQAITEMKNLQDGGSMAEHITNVRTWVSSALTDEGTCTDGFE---EVKVNKETKK 182

Query: 184 EVKKSVAVVVRHISNALSLLNTFKY 200
           +V K V  +    SN L+L+   +Y
Sbjct: 183 KVNKVVEELATTTSNTLALITNLRY 204

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TAIR 10
Match: AT4G25250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 95.1 bits (235), Expect = 6.7e-20
Identity = 58/191 (30.37%), Postives = 111/191 (58.12%), Query Frame = 0


Query: 10  LTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQE 69
           LTL  F++ +      A+     +    Y+K AC +T YP +C   L+ ++S  K    +
Sbjct: 9   LTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKSDPIK 68

Query: 70  FCRATMASSLKTTRDATSIISKLSRQKMS--TYEAKVIRDCVDNLQDSTDELQRAIAAIK 129
            C  ++  ++K+ ++AT ++S L ++  +  ++E  +++DCVD ++D+ DEL++A+A +K
Sbjct: 69  LCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVAEMK 128

Query: 130 NL-SRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRH 189
            +    K     L ++KTW+S+A TD  TC+DG   G   +V  + K++VKK+++ + + 
Sbjct: 129 YVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKKAISELSKT 188

Query: 190 ISNALSLLNTF 198
            SN L+LL  +
Sbjct: 189 TSNTLALLTHY 196

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 4.8e-18
Identity = 57/189 (30.16%), Postives = 101/189 (53.44%), Query Frame = 0


Query: 9   LLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQ 68
           L T    +++        ++++ T     ++K +C  T YPRLC  SL+ HAS  + + +
Sbjct: 14  LTTFLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPK 73

Query: 69  EFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQRAIAAIKN 128
               A +  +L + +  ++++ +LS  ++   E   +RDCV+ L D+ +EL+++I  +  
Sbjct: 74  LMAHAALNITLASAKVTSAMMVRLSNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQ 133

Query: 129 LSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHIS 188
           LS   +    +  I+TW+SAA TD  TC+DG     G  +  KVK  V+  + V+    S
Sbjct: 134 LS-GSNYEVYISDIQTWVSAALTDVNTCTDGFE---GEDMDGKVKVLVRGRILVIAHLTS 193

Query: 189 NALSLLNTF 198
           NAL+L+N F
Sbjct: 194 NALALINHF 198

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.0e-15
Identity = 60/200 (30.00%), Postives = 103/200 (51.50%), Query Frame = 0


Query: 11  TLFFFVSITFTTSVVASSSSVTRPVKP------YIKKACKTTPYPRLCEISLTLHASQTK 70
           TLF F+    +T+++ +SSS  R          +I+ +CK T YP +C  SLT +A+  +
Sbjct: 8   TLFLFL---LSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQ 67

Query: 71  RTQQEFCRATMASSLKTTRDATSIISKLSR-QKMSTYEAKVIRDCVDNLQDSTDELQRAI 130
            + +      +  ++   +     + +L R   +   E + ++DC++ + D+ D L  +I
Sbjct: 68  TSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSI 127

Query: 131 AAIKNLSRAKDVN---FQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 190
             +K    AK  +   F + + +TW SAA T+A TCSDG    AGR +  +VK  V+  +
Sbjct: 128 HEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGF---AGRVMDGRVKNSVRARI 187

Query: 191 AVVVRHISNALSLLNTFKYK 201
             + R  SNAL+L+N F  K
Sbjct: 188 LNLGRGTSNALALINAFAKK 201

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g03680.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	1.4e-22	34.22	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB38	9.5e-19	30.37	Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1	[more]
Q9STY5	1.4e-14	30.00	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SI72	2.4e-14	30.35	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SI74	6.6e-12	30.22	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023542401.1	9.73e-132	100.00	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
KAG7030832.1	2.11e-126	96.02	hypothetical protein SDJN02_04869, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022942851.1	1.22e-125	95.52	21 kDa protein-like [Cucurbita moschata]	[more]
XP_022987359.1	1.66e-123	93.53	21 kDa protein-like [Cucurbita maxima]	[more]
XP_038882390.1	1.94e-90	71.14	21 kDa protein-like [Benincasa hispida]	[more]

BLAST of Cp4.1LG01g03680.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FQ13	5.90e-126	95.52	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111447758 PE=4 SV=1	[more]
A0A6J1JIN9	8.04e-124	93.53	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111484936 PE=4 SV=1	[more]
A0A1S3AXZ9	6.93e-86	70.30	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1	[more]
A0A0A0L671	6.80e-84	67.49	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 S...	[more]
A0A6J1J533	2.40e-80	67.35	pectinesterase inhibitor 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483499 PE=4...	[more]

BLAST of Cp4.1LG01g03680.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62350.1	2.5e-22	32.34	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G51520.1	7.9e-21	30.73	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25250.1	6.7e-20	30.37	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62360.1	4.8e-18	30.16	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	1.0e-15	30.00	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 109..132
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 9..194
None	No IPR available	PANTHER	PTHR31080:SF15	PECTINESTERASE INHIBITOR 4	coord: 9..194
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 42..197 e-value: 1.3221E-34 score: 118.31
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 34..193 e-value: 1.7E-37 score: 140.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 38..193 e-value: 8.4E-29 score: 100.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 12..196 e-value: 2.3E-31 score: 106.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 36..197 e-value: 1.5E-30 score: 108.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 37..197

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG01g03680	Cp4.1LG01g03680	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG01g03680.1:exon:002	Cp4.1LG01g03680.1:exon:002	exon
Cp4.1LG01g03680.1:exon:001	Cp4.1LG01g03680.1:exon:001	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG01g03680.1:cds:002	Cp4.1LG01g03680.1:cds:002	CDS
Cp4.1LG01g03680.1:cds:001	Cp4.1LG01g03680.1:cds:001	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG01g03680.1	Cp4.1LG01g03680.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity