CmoCh20G006530.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G006530.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionO-methyltransferase family protein
LocationCmo_Chr20: 3192423 .. 3196760 (+)
Sequence length2619
RNA-Seq ExpressionCmoCh20G006530.1
SyntenyCmoCh20G006530.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAGAAGGTGGATGAGCTGCTTCAATCCCAAGCTCATGTTTGGAACCATGCTTTGAAATTTTTCGACTCCATGTCTCTTAGATGTGCTGTTGAATTGGGCATACCGGATGTTATCCATACCCACGCCCAACCCATGAGCATTCAACCTTCAAAGGCTCAACACCTTGGCCGCCTCATGCGCCTCCTCGTCCATTCTGGCTTCTTTGATGCCAACGAGCAAGAGGACGTGAAATACAGCCTCACGCCGTCCTCTCGCCGTCTCCTCCGCCACACCCACACACACACTCGCACCACTTTCCAAATCATACCCTTCTTGTTTCTTAGTTTGGATAAAGCTACAATTGCTTCTATGGGAGCTTTGAGCGGTTGGCTTTGTAGCTCAGATCAAGACCATAACTCCAGTTCCTTTGAAATGGCAAACGGGAAGCCCATGTGGGACTACTCTGCTCAGGAACCAAGTTTTGGCAATTTGTTTCATCAGGCAATGGTTTGCGATTCTGAATTTATTGGTAAAATTGTGACGGAGAAGTGTAGCAGAGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCAGCAACCAACTGCAAAAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGGTAATGTTCTTAGATTAAGAAATTAGATATGTTTTTAACACTAAATTATTAATGGGTTTTTGATAAACAGTGGATATTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCAGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAAGACAGGGAGTCCACTGAAACTCAACTGTTCTTTGATGTTTTGATGATGGCCAATCTAAACGGCAAGGAGAGAAACGAGAGAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGGTAACTTCTTGAGCTGTACCCTTAACTCAAAAGTGTTGTTATTTGCTGCTTAATTATGCTTCTTCTTGTTGTGGGTTCGTAGAACTTGTGGTGTTGGTTTCAGGAAACCAGAAACAAACCATAATAAAGTTGTTATATTATTAATAGCTATTACTTATATACTCTTTTCGCATTCTTATCTTACTAGCTATCTATGACTTTGTCTCGGGTTCTAGAGAGTGTGGACTAAGCTGCAACTCTCTTTTCTACAACTCAGGTGGCTTTTCTATCTTATGCACTGGATGATTAGTGTCTTGGAATGAGGCGCGACCTCACGAGTAAAACTGAAGGAACAATATGGCTGGATCTAAATTAGTATCGTTCTTTGAACATCATCCGTGGGGGTTAGCTGACAACCATCCCACGAGACCATACTACCACTAAAATCGAGGGGTTGTGATCTCTTGTGTGCTGGTCAGCTACTAGATCATCCTACGTGATCCCACGCTCCCCCACTCAGGGATGGGTTTCTACAGCTTAGGGGTCCTATTCAGAAGACCACTTAGTTATCACCACTAGGTCAGGGCACAAATCATACTGCCTTGTCCGACTCTACCTGGCTCAATGATCTCAGTATCGGAGTACTGTCTCGACAGTACGATATCGGAGCAGAAAGCGAAGTGGAGGTTCTTGTAACACTATCCACACTACTCATGTCCGGCTAGCTACCTCGGTTGTCTATAGTTACCGTAGTGTCTTACTAGGTCTTTCTAGGGCGGATCCTGTGTCTTAGACTTGGATTCATTCATCTATAGAATTCTCATGCAAGGTCTCTCAAACCCGTCGTGGTCTTGAATATGGTTTGGCTCCGCTATTCATCCCTTCGTGGGCCTGAATGCATAGTATTCTAGAGGTATCTCATTCTAGGTCTTTGTTTAGTCTACTTAGTCTCAAGTGCCCTAGTCTAGGCTCGTCGTTTCTTCCTATGCTCAACAAGAAAGACAACACAAGCAATCATGCACTTAAGTTAACATGCAACAACTCATGCAATATTAACAAATAAGCTCGATGAGAACATAACGTCCATCTTTACCCAGAGCGCAGATTTTGCAAACTTCCATTCTTCCTGTATCATGCATACAATATCTCAATCTCATTCTAAAGCAAATTCATAGTCAATCGATTCTAGTTACCATTGTTTTTCTTATCTAGTTCTTTTCTATAGCTTATATTTTAATATGTTAGGTCCAGCTAACTAGGATTTATTAGTTTGACAACATGCATACTTATTCCAAGCGGTTCCGACAAGTACCACTCTTAGAATCTAAAAGTTTTGTCGTTGAAGAGAGATTAGAACGGTGTATGAGAGTTCGCTTATTTAAGCAATACTCGCCTAATTTGATAACCATTCCTCTTTAAGAATGAACCAAATTTTAATAACATTTAGGGTAGAAAGGTACTTTCTTTTCTTGGGAAGGCCCTAGTCATTTATGATTGTGGAAGGGAGAGATCAGGGCAGTGGATAGGAGTTTAAATATGAAATCACATCGGATGACGATGGAATCTGCAAGTTGTGAGGAAATTAATATCATAAATACAGCTTCCGGAGGTTGCCTGATTTCAGATCTGATCCCTAACAAAATCTAGATGGAAGAGAAGGTGGATGAGCTGCTTCAATCCCAAGCTCATGTTTGGAACCATGCTTTGAAACTTACGAACTCCATGGCTCTTAAATGTGCTGTTGAATTGGGCATACCGGATGTTATCCATACCCACGCCCAACCCATGAGCCTCTCGGATTTAGTGGCGGCGCTCCATATTCAACCTTCAAAGGCTCAATACCTTGGCCGCCTCATGCGCCCCCTCGTTCATTCTGGCTTCTTTGATGCGGACGAGCAAGAAGACGTGAGATACAGCCTCACGCCATCCTCTCGCCTTCTCCTCCGCCACACACACACCTACACCACTTTCCAAATGATACCCTTCTTGTGTATTGCTTTGGATAAAGCTACAATTGCTTCTTTGGGAGCTTTGAGCAGTTGGCTCTGTAGCTCAGACCAAGGCCATAACTCCAATTCCTTTGAAATGGCAAACGGGAAGTCCATATGGGAGTATGCTGCTCAGGAACCAAATTTTGGCAATTTGTTTCAACAGATAATGGTTTGTGATTCTGAATTGATTGGTAAAATAGTGATTGAGAAGTGTAGCGGTGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCTACAACCAAATGTAAGAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGGTAATGTTCTTATATTAACAAATTAGATACGTTTTTAACACTAAATTATTAATGGATTTTGATAATCAGTGGGTGTTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGGCGAAGGGGGAAAGTTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAAGACAGGGAGTCCACTGAAACTCAACTGTTCTTCGATGTATTGATGATGGTCAATCTAAACGGCAAGGAGAGAAATGAGAAAGAATGGAAGTATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAATTTGTAAACTGGGTTTAAGGTCCCTTATTGAAGTGTACCCTTAATTGAAAGTGCCTTTGCTGCTTAAGTTTGGTTATGGGTGAAACTTACGGTGTGCGTGTGGTGAAACAATGTATAATAAAGTTTGTATATTATGTGGGTTGGAAAAGTGTTGTAAAATGGCCCAATTTTTGTTGGGCTGTGTATTGAGGATGGCTTAAGAGATGCATTTTGTGTCGTCGCTTGCCCAATCTCCCCGCTCCAACCAAAGGCTAGCTCCATGCATGCCCAGTGTCACGGTTATGCTTGTCTAAGGCGTGTTGTCCATGATCGCATGTCCATGACAAGTCCAACCCTTTATGTCTTTTCATATTCTGGTGTTTTACTTTTGTATTTGCAGGAGGTATGACGTTTCAGAAATATTATGTCTAGGCCTGTTAGACATGAAATGCCTCGTAATGTAATTTAGGGAGATACGACTAGTTGTCAACCTCTCCCTGCCCAAGGTTATATATTGTGAGCCG

mRNA sequence

ATGGAAGAGAAGGTGGATGAGCTGCTTCAATCCCAAGCTCATGTTTGGAACCATGCTTTGAAATTTTTCGACTCCATGTCTCTTAGATGTGCTGTTGAATTGGGCATACCGGATGTTATCCATACCCACGCCCAACCCATGAGCATTCAACCTTCAAAGGCTCAACACCTTGGCCGCCTCATGCGCCTCCTCGTCCATTCTGGCTTCTTTGATGCCAACGAGCAAGAGGACGTGAAATACAGCCTCACGCCGTCCTCTCGCCGTCTCCTCCGCCACACCCACACACACACTCGCACCACTTTCCAAATCATACCCTTCTTGTTTCTTAGTTTGGATAAAGCTACAATTGCTTCTATGGGAGCTTTGAGCGGTTGGCTTTGTAGCTCAGATCAAGACCATAACTCCAGTTCCTTTGAAATGGCAAACGGGAAGCCCATGTGGGACTACTCTGCTCAGGAACCAAGTTTTGGCAATTTGTTTCATCAGGCAATGGTTTGCGATTCTGAATTTATTGGTAAAATTGTGACGGAGAAGTGTAGCAGAGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCAGCAACCAACTGCAAAAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGTGGATATTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCAGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAAGACAGGGAGTCCACTGAAACTCAACTGTTCTTTGATGTTTTGATGATGGCCAATCTAAACGGCAAGGAGAGAAACGAGAGAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGAACTTGTGGTGTTGGTTTCAGGAAACCAGAAACAAACCATAATAAAATGGAAGAGAAGGTGGATGAGCTGCTTCAATCCCAAGCTCATGTTTGGAACCATGCTTTGAAACTTACGAACTCCATGGCTCTTAAATGTGCTGTTGAATTGGGCATACCGGATGTTATCCATACCCACGCCCAACCCATGAGCCTCTCGGATTTAGTGGCGGCGCTCCATATTCAACCTTCAAAGGCTCAATACCTTGGCCGCCTCATGCGCCCCCTCGTTCATTCTGGCTTCTTTGATGCGGACGAGCAAGAAGACGTGAGATACAGCCTCACGCCATCCTCTCGCCTTCTCCTCCGCCACACACACACCTACACCACTTTCCAAATGATACCCTTCTTGTGTATTGCTTTGGATAAAGCTACAATTGCTTCTTTGGGAGCTTTGAGCAGTTGGCTCTGTAGCTCAGACCAAGGCCATAACTCCAATTCCTTTGAAATGGCAAACGGGAAGTCCATATGGGAGTATGCTGCTCAGGAACCAAATTTTGGCAATTTGTTTCAACAGATAATGGTTTGTGATTCTGAATTGATTGGTAAAATAGTGATTGAGAAGTGTAGCGGTGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCTACAACCAAATGTAAGAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGTGGGTGTTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGGCGAAGGGGGAAAGTTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAAGACAGGGAGTCCACTGAAACTCAACTGTTCTTCGATGTATTGATGATGGTCAATCTAAACGGCAAGGAGAGAAATGAGAAAGAATGGAAGTATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAATTTGTAAACTGGGTTTAAGGTCCCTTATTGAAGTGTACCCTTAATTGAAAGTGCCTTTGCTGCTTAAGTTTGGTTATGGGTGAAACTTACGGTGTGCGTGTGGTGAAACAATGTATAATAAAGTTTGTATATTATGTGGGTTGGAAAAGTGTTGTAAAATGGCCCAATTTTTGTTGGGCTGTGTATTGAGGATGGCTTAAGAGATGCATTTTGTGTCGTCGCTTGCCCAATCTCCCCGCTCCAACCAAAGGCTAGCTCCATGCATGCCCAGTGTCACGGTTATGCTTGTCTAAGGCGTGTTGTCCATGATCGCATGTCCATGACAAGTCCAACCCTTTATGTCTTTTCATATTCTGGTGTTTTACTTTTGTATTTGCAGGAGGTATGACGTTTCAGAAATATTATGTCTAGGCCTGTTAGACATGAAATGCCTCGTAATGTAATTTAGGGAGATACGACTAGTTGTCAACCTCTCCCTGCCCAAGGTTATATATTGTGAGCCG

Coding sequence (CDS)

ATGGAAGAGAAGGTGGATGAGCTGCTTCAATCCCAAGCTCATGTTTGGAACCATGCTTTGAAATTTTTCGACTCCATGTCTCTTAGATGTGCTGTTGAATTGGGCATACCGGATGTTATCCATACCCACGCCCAACCCATGAGCATTCAACCTTCAAAGGCTCAACACCTTGGCCGCCTCATGCGCCTCCTCGTCCATTCTGGCTTCTTTGATGCCAACGAGCAAGAGGACGTGAAATACAGCCTCACGCCGTCCTCTCGCCGTCTCCTCCGCCACACCCACACACACACTCGCACCACTTTCCAAATCATACCCTTCTTGTTTCTTAGTTTGGATAAAGCTACAATTGCTTCTATGGGAGCTTTGAGCGGTTGGCTTTGTAGCTCAGATCAAGACCATAACTCCAGTTCCTTTGAAATGGCAAACGGGAAGCCCATGTGGGACTACTCTGCTCAGGAACCAAGTTTTGGCAATTTGTTTCATCAGGCAATGGTTTGCGATTCTGAATTTATTGGTAAAATTGTGACGGAGAAGTGTAGCAGAGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCAGCAACCAACTGCAAAAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGTGGATATTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGCAGAAGGTGGAAAATTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAAGACAGGGAGTCCACTGAAACTCAACTGTTCTTTGATGTTTTGATGATGGCCAATCTAAACGGCAAGGAGAGAAACGAGAGAGAATGGAAGAATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAGTTTCTAAGTTGGGTTTAAGAACTTGTGGTGTTGGTTTCAGGAAACCAGAAACAAACCATAATAAAATGGAAGAGAAGGTGGATGAGCTGCTTCAATCCCAAGCTCATGTTTGGAACCATGCTTTGAAACTTACGAACTCCATGGCTCTTAAATGTGCTGTTGAATTGGGCATACCGGATGTTATCCATACCCACGCCCAACCCATGAGCCTCTCGGATTTAGTGGCGGCGCTCCATATTCAACCTTCAAAGGCTCAATACCTTGGCCGCCTCATGCGCCCCCTCGTTCATTCTGGCTTCTTTGATGCGGACGAGCAAGAAGACGTGAGATACAGCCTCACGCCATCCTCTCGCCTTCTCCTCCGCCACACACACACCTACACCACTTTCCAAATGATACCCTTCTTGTGTATTGCTTTGGATAAAGCTACAATTGCTTCTTTGGGAGCTTTGAGCAGTTGGCTCTGTAGCTCAGACCAAGGCCATAACTCCAATTCCTTTGAAATGGCAAACGGGAAGTCCATATGGGAGTATGCTGCTCAGGAACCAAATTTTGGCAATTTGTTTCAACAGATAATGGTTTGTGATTCTGAATTGATTGGTAAAATAGTGATTGAGAAGTGTAGCGGTGTGTTTGAAGGGTTGAAGTCATTGGTTGATGTTGGTGGTGGCACAGGCGTTATGGCCAACGCTATTGCAGAGGCCTTCCCGCACATCACTTGCTCTGTATTTGATCTCCCACAAGTGGTTTCCAACCTACAACCAAATGTAAGAAACTTGAGCTTTATTGGAGGAGACATGCTTGAATCAAAAATCCCTCCCGCAAATGCTGTTATGCTCAAGTGGGTGTTACACAATTGGGATGATGAACAAAGCATTAAGATATTGAAGAAGTGTAAAGATGCAATTCCAAGCAGAGGCGAAGGGGGAAAGTTGATCATCATAGACATGGTGTTGGACAACGAAGTAGAAGACAGGGAGTCCACTGAAACTCAACTGTTCTTCGATGTATTGATGATGGTCAATCTAAACGGCAAGGAGAGAAATGAGAAAGAATGGAAGTATCTGTTTATGGAAGCAGGTTTCAGTGATTACACCATAATTTGTAAACTGGGTTTAAGGTCCCTTATTGAAGTGTACCCTTAA

Protein sequence

MEEKVDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMSIQPSKAQHLGRLMRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSLDKATIASMGALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFIGKIVTEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNLSFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDRESTETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLRTCGVGFRKPETNHNKMEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQPSKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLDNEVEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVYP
Homology
BLAST of CmoCh20G006530.1 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.1e-96
Identity = 188/368 (51.09%), Postives = 245/368 (66.58%), Query Frame = 0

Query: 362 EEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQPS 421
           EE   E+L +QAH+WN      NSM+LKCA++LGIPD+I+ H +PM++S L  AL I   
Sbjct: 20  EENSTEILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHGKPMTISQLTLALPINRK 79

Query: 422 KAQYLGRLMRPLVHSGFF-----DADEQ---EDVRYSLTPSSRLLLRHTHTYTTFQMIPF 481
           K+  + RLMR L+HSGFF     +  E+   E+  Y +T +S+LLL+      T    PF
Sbjct: 80  KSPCVYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVT----PF 139

Query: 482 LCIALDKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVC 541
           L   LD         LS+W     Q  +   F+ ANG + W+Y + EP     F   M  
Sbjct: 140 LLAMLDPVMTKPWDFLSNWF----QNGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMAS 199

Query: 542 DSELIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQP 601
           D+ L+  +VIEKC GVFEG++SLVDVGGGTG +A++IA AFPHI C+VFDLP VV++LQ 
Sbjct: 200 DARLVTSVVIEKCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQG 259

Query: 602 NVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAI-PSRGEGGKLII 661
              NL+F+GGDM    +P    V+LKW+LH+W+DE+S+KILKKCK+AI  S  +GGK+II
Sbjct: 260 G-NNLNFVGGDMFVD-VPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVII 319

Query: 662 IDMVLDNEV-EDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGL 720
           IDM ++NE  ED ES ETQLFFD+LMM  + G+ERNEKEW  LF +AGFS+Y I   LGL
Sbjct: 320 IDMKVENEKDEDDESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILGL 377

BLAST of CmoCh20G006530.1 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 345.5 bits (885), Expect = 1.4e-93
Identity = 176/357 (49.30%), Postives = 238/357 (66.67%), Query Frame = 0

Query: 367 ELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQPSKAQYL 426
           ELL +QAHVWNH      SM+LKCA++LGIPD+IH H +PM+L +LVA L + P ++Q +
Sbjct: 11  ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHGKPMTLPELVAKLPVHPKRSQCV 70

Query: 427 GRLMRPLVHSGFFDADE----QEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDKAT 486
            RLMR LVHSGF  A      +E+  Y LT +SRLLL       +  + P +   LD   
Sbjct: 71  YRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLMD----DSLSIRPLVLAMLDPIL 130

Query: 487 IASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGKIV 546
                 LS+W     Q  +   F  A  +S W+YA  EP   N F + M  D+ L+  ++
Sbjct: 131 TKPWHYLSAWF----QNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTSVL 190

Query: 547 IEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIG 606
           +++  GVF GL SLVDVGGGTG +A AIA AFPH+ C+V DL  VV+ LQ + +NL++  
Sbjct: 191 LKEGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGS-KNLNYFA 250

Query: 607 GDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLDNEVE 666
           GDM E+ IPPA+A++LKW+LH+W +E+ +KILK+C++AIPS+  GGK+IIIDM++     
Sbjct: 251 GDMFEA-IPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQG 310

Query: 667 DRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVYP 720
           D +STETQLFFD+ MM+   G+ER+E EW+ LF++AGFS Y I   LGLRSLIEVYP
Sbjct: 311 DYKSTETQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEVYP 357

BLAST of CmoCh20G006530.1 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 4.8e-89
Identity = 177/369 (47.97%), Postives = 243/369 (65.85%), Query Frame = 0

Query: 357 NHNKMEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTH-AQPMSLSDLVAA 416
           N N   +  +ELL +QAH++NH     +SMALKCAVEL IP+++H H  + ++LS+LV A
Sbjct: 13  NGNGYGDSGEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTLSELVQA 72

Query: 417 LHIQPSKAQYLGRLMRPLVHSGFFD----ADEQEDVRYSLTPSSRLLLRHTHTYTTFQMI 476
           L I  +K+  L RL+R LVHSGFF       E ++  Y  T SS+LLL++        M 
Sbjct: 73  LQIPQAKSACLYRLLRILVHSGFFAITKIQSEGDEEGYLPTLSSKLLLKN----HPMSMS 132

Query: 477 PFLCIALDKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIM 536
           P L   ++   +A +   S W   SD   +   FE  +G S+W+Y  + P+   +F + M
Sbjct: 133 PCLLGLVNPTMVAPMHFFSDWFKRSD---DMTPFEATHGASLWKYFGETPHMAEIFNEAM 192

Query: 537 VCDSELIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNL 596
            C++ L   +V+++C G  EG+ SLVDVGGGTG +  AIAEAFP++ C+V DLPQVV NL
Sbjct: 193 GCETRLAMSVVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNL 252

Query: 597 QPNVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLI 656
           + +  NL F+ GDM +  IPPA+ V LKW+LH+W+DE+ IKILK+CK+AIPS+ EGGKLI
Sbjct: 253 KGS-NNLEFVSGDMFQF-IPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLI 312

Query: 657 IIDMVL-DNEVEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLG 716
           IIDMV+ D+     ESTETQLF+D+ +M  L G ER E EWK LF+ AGF+ Y I   LG
Sbjct: 313 IIDMVVNDHNKGSYESTETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVLG 372

Query: 717 LRSLIEVYP 720
           L+S+IEV+P
Sbjct: 373 LKSIIEVFP 372

BLAST of CmoCh20G006530.1 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 5.5e-85
Identity = 168/365 (46.03%), Postives = 233/365 (63.84%), Query Frame = 0

Query: 367 ELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQPSKAQYL 426
           ELL++QAHVWNH     NSM+LKCA++LGIPD IH H  P++LS L  AL+I  +K+  L
Sbjct: 12  ELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHGNPITLSQLADALNINKAKSHGL 71

Query: 427 GRLMRPLVHSGFFDA-----------DEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLC 486
            RLMR LVHSGFFD            +E+E+  YSLTP+SRLLLR         + PF  
Sbjct: 72  FRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRS----EPLSVAPFAL 131

Query: 487 IALDKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDS 546
              D     +   LS W     +     +F+   G +  EYA  +     LF + M CD+
Sbjct: 132 AMSDPVYTETWHHLSEWF----RNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDA 191

Query: 547 ELIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNV 606
             +  I+  +C  +F+GL+S+VDVGGGTG  A  IA AFP + C+V DLP VV  L+ + 
Sbjct: 192 GFVNSILTTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGS- 251

Query: 607 RNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIP-SRGEGGKLIIID 666
            NLSF+ GDM +  IP A+A+ +K++LH+W+DE+ +KILKKCK+AI  S     K+I+++
Sbjct: 252 ENLSFVSGDMFDF-IPHADAIFMKFILHDWNDEECVKILKKCKEAISRSNNSCRKIILVE 311

Query: 667 MVLDNEVEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSL 720
           +V+++E E  E+TET+LFFD+ M+  + GKER+EKEW  LF +AGF++Y I   LGLRS+
Sbjct: 312 IVMEDEKETHEATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTNYKITRVLGLRSV 366

BLAST of CmoCh20G006530.1 vs. ExPASy Swiss-Prot
Match: Q8GSN1 (Myricetin O-methyltransferase OS=Catharanthus roseus OX=4058 PE=1 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 7.1e-85
Identity = 171/361 (47.37%), Postives = 237/361 (65.65%), Query Frame = 0

Query: 361 MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP 420
           ME +  E+  +QAH +      T+  +LKCAV+LGIPD IH+H +PM+LSDL  +L I P
Sbjct: 1   MELQSSEIRNAQAHFFTQVFSFTSMSSLKCAVQLGIPDAIHSHGKPMALSDLTNSLPINP 60

Query: 421 SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDK 480
           SKA Y+ RLMR LV +G+F   E+E   YSLTP +RLLL++    +   ++    IA  K
Sbjct: 61  SKAPYIYRLMRILVAAGYF--SEEEKNVYSLTPFTRLLLKNDPLNSISMVLGVNQIAELK 120

Query: 481 ATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGK 540
           A      A+S W     Q  +  +FE A+GK+ W++ A E  +G  F  +M  DS L+ K
Sbjct: 121 A----WNAMSEWF----QNEDLTAFETAHGKNFWDFGA-EDKYGKNFDGVMAADSILVSK 180

Query: 541 IVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSF 600
           ++I + + +FEGL SLVDVGGGTG +A AIA++FP + C+VFDLP VV+NL+ +  NL F
Sbjct: 181 MLIPEFNYLFEGLDSLVDVGGGTGTIAKAIAKSFPDLKCTVFDLPHVVANLE-STENLEF 240

Query: 601 IGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVL--D 660
           +GGDM E KIP ANA++LKW+LH+W DE+ +K+LK C+ AIP + +GGK+I+I+ VL   
Sbjct: 241 VGGDMFE-KIPSANAILLKWILHDWKDEECVKVLKMCRKAIPEKEKGGKVILIETVLMDS 300

Query: 661 NEVEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVY 720
            + E+ E+ + Q+  D+ MMV    KER E+EW  LF EAGFS Y I   +  RS IEVY
Sbjct: 301 KKHENEEAVKAQISSDIDMMVFFTAKERTEEEWATLFREAGFSGYKIFPMIDFRSPIEVY 348

BLAST of CmoCh20G006530.1 vs. ExPASy TrEMBL
Match: A0A6J1FXQ6 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111448676 PE=3 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 3.8e-206
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0

Query: 361 MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP 420
           MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP
Sbjct: 1   MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP 60

Query: 421 SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDK 480
           SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDK
Sbjct: 61  SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDK 120

Query: 481 ATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGK 540
           ATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGK
Sbjct: 121 ATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGK 180

Query: 541 IVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSF 600
           IVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSF
Sbjct: 181 IVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSF 240

Query: 601 IGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLDNE 660
           IGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLDNE
Sbjct: 241 IGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLDNE 300

Query: 661 VEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVYP 720
           VEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVYP
Sbjct: 301 VEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVYP 359

BLAST of CmoCh20G006530.1 vs. ExPASy TrEMBL
Match: A0A6J1FYH4 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111448677 PE=3 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 1.3e-198
Identity = 345/345 (100.00%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   MEEKVDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMSIQPSKAQHLGRL 60
           MEEKVDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMSIQPSKAQHLGRL
Sbjct: 1   MEEKVDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMSIQPSKAQHLGRL 60

Query: 61  MRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSLDKATIASMG 120
           MRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSLDKATIASMG
Sbjct: 61  MRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSLDKATIASMG 120

Query: 121 ALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFIGKIVTEKCS 180
           ALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFIGKIVTEKCS
Sbjct: 121 ALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFIGKIVTEKCS 180

Query: 181 RVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNLSFIGGDMLE 240
           RVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNLSFIGGDMLE
Sbjct: 181 RVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNLSFIGGDMLE 240

Query: 241 SKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDREST 300
           SKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDREST
Sbjct: 241 SKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDREST 300

Query: 301 ETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLR 346
           ETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLR
Sbjct: 301 ETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLR 345

BLAST of CmoCh20G006530.1 vs. ExPASy TrEMBL
Match: A0A6J1JFR9 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111484102 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 2.0e-183
Identity = 321/361 (88.92%), Postives = 338/361 (93.63%), Query Frame = 0

Query: 361 MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP 420
           MEEK+DELLQSQAHVWNHALK  NSMALKCAVELGIPDVIH+HAQPM+LSDLVAAL IQP
Sbjct: 1   MEEKLDELLQSQAHVWNHALKFMNSMALKCAVELGIPDVIHSHAQPMTLSDLVAALRIQP 60

Query: 421 SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRH--THTYTTFQMIPFLCIAL 480
           SKAQYLGRLMR LVHSGFFDADEQEDVRYSLTPSSRLLLRH  TH++TTFQ+IPFL + L
Sbjct: 61  SKAQYLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHSHTTFQIIPFLYLGL 120

Query: 481 DKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELI 540
           DK TIASLGA S WLCSSDQ HNSNSFEMANGK IWEYAAQEP+FGNLFQQ MVCDSE+I
Sbjct: 121 DKTTIASLGAFSGWLCSSDQDHNSNSFEMANGKPIWEYAAQEPSFGNLFQQTMVCDSEMI 180

Query: 541 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 600
           GKIV EKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITC+VFDLPQVVSN Q   +NL
Sbjct: 181 GKIVTEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCTVFDLPQVVSNQQRTAKNL 240

Query: 601 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLD 660
            FIGGDMLE+KIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSR EGGKL+IIDMVLD
Sbjct: 241 RFIGGDMLEAKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLVIIDMVLD 300

Query: 661 NEVEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVY 720
           NE+E+RESTETQL FDVLMMVNL+GKERNEKEWK LFMEAGFSDYTI+CKLGLRSLIEVY
Sbjct: 301 NEMEERESTETQLLFDVLMMVNLHGKERNEKEWKNLFMEAGFSDYTIVCKLGLRSLIEVY 360

BLAST of CmoCh20G006530.1 vs. ExPASy TrEMBL
Match: A0A6N2MMG0 (Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS385670 PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 5.9e-183
Identity = 359/744 (48.25%), Postives = 469/744 (63.04%), Query Frame = 0

Query: 2   EEKVDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMS---------IQPS 61
           EE   ELLQ+Q  VWNH   F +SM+L+CAV+LGIPDVIH H +PM+         I PS
Sbjct: 6   EESASELLQAQTQVWNHIFNFINSMTLKCAVQLGIPDVIHKHGKPMTLSELVSSLPIHPS 65

Query: 62  KAQHLGRLMRLLVHSGFFDANE----QEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLF 121
           K Q++ R+MR+LVHSGFF             Y L+ S R LL  +    R      P L 
Sbjct: 66  KTQYVHRIMRILVHSGFFSQQNLNGGPNQEAYFLSQSGRLLLEDSPLSMR------PLLL 125

Query: 122 LSLDKATIASMGALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDS 181
           + LD         LS W     ++   ++F +A+G+ +W+Y+ Q+P   NLF+ AM  DS
Sbjct: 126 MLLDPVLTKPWDCLSTWF----RNDEVTAFSVAHGRTLWEYAGQDPRLNNLFNAAMANDS 185

Query: 182 EFIGKIVTEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTA 241
             + K++  KC  +FEG+ SLVDVGGG G MA AI+EAFPHI C+VFDLP VVS+ Q  +
Sbjct: 186 VLVSKVIVSKCKGIFEGVSSLVDVGGGVGTMATAISEAFPHIHCTVFDLPHVVSDLQ-GS 245

Query: 242 KNLSFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAI---PSRAEGGKLII 301
           KNL +  GDMLE+ +PPA+A++LKWILH+W DE  IKIL++CK AI     + + GK+II
Sbjct: 246 KNLEYAAGDMLEA-VPPADALLLKWILHDWSDEDCIKILQQCKQAIMRNEKQKKVGKVII 305

Query: 302 IDMVLDNEVEDRESTETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLR 361
           IDMV +N+  D  S ET+LFFD+ MM  +NG ERNE+EW  LF +AGF +Y I   + L 
Sbjct: 306 IDMVRENQNRDEMSAETRLFFDLEMMVTVNGMERNEKEWGKLFFDAGFLNYKIHPVIVL- 365

Query: 362 TCGVGFRKPETNHNKMEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQ 421
                      +++K++               + +   NSM LKCAV+LGIPD IH H +
Sbjct: 366 ----------VSYSKLK-------------LTYGIIFINSMTLKCAVQLGIPDAIHKHGK 425

Query: 422 PMSLSDLVAALHIQPSKAQYLGRLMRPLVHSGFFDA-------DEQEDVRYSLTPSSRLL 481
           PM+LS LV+AL I PSK QY+ RLMR LVHSGFF         D+QE   YSL+ S+RLL
Sbjct: 426 PMTLSQLVSALPIHPSKTQYVHRLMRILVHSGFFSQQNLNGINDDQE--VYSLSQSARLL 485

Query: 482 LRHTHTYTTFQMIPFLCIALDKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAA 541
           L      +   M P L I LD         LS+W     +     +F +A+GK+IWEY  
Sbjct: 486 LED----SPLSMRPLLLILLDPVVTKPWDCLSTWF----RNDEVTAFSVAHGKTIWEYTG 545

Query: 542 QEPNFGNLFQQIMVCDSELIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHIT 601
           Q+P   NLF + M  DS L+ K+++ KC G+      LVDVGGGTG MA AI+EAFPHI 
Sbjct: 546 QDPRLSNLFNEAMASDSVLVSKVIVSKCKGMI-----LVDVGGGTGTMAKAISEAFPHID 605

Query: 602 CSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCK 661
           C+VFDLP+VVS+LQ + +NL ++GGDM E  +PPA+AV+LKW+LH+W DE  IKIL++CK
Sbjct: 606 CTVFDLPRVVSDLQGS-KNLKYVGGDMFE-VVPPADAVLLKWILHDWSDEDCIKILQRCK 665

Query: 662 DAIPSRGEG--GKLIIIDMVLDNEVEDRE-STETQLFFDVLMMVNLNGKERNEKEWKYLF 720
            AI   G+   GK+IIIDMV +N+  D   STETQLFFD+ MMV +NG ERNEKEW  LF
Sbjct: 666 QAIMRNGKQKVGKVIIIDMVRENKNRDEAVSTETQLFFDLEMMVTVNGMERNEKEWGKLF 696

BLAST of CmoCh20G006530.1 vs. ExPASy TrEMBL
Match: A0A6N2LSG2 (Uncharacterized protein (Fragment) OS=Salix viminalis OX=40686 GN=SVIM_LOCUS271561 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 8.8e-179
Identity = 337/727 (46.35%), Postives = 464/727 (63.82%), Query Frame = 0

Query: 7   ELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMS---------IQPSKAQHL 66
           ELLQ+QAHVWNH   F +SMSL+CAVELGIPD+I  H +PM+         I P+KA  +
Sbjct: 12  ELLQAQAHVWNHIFNFINSMSLKCAVELGIPDIILNHGKPMTLSELVAVLPIHPTKAPFV 71

Query: 67  GRLMRLLVHSGFF----DANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSLDK 126
            RLMR+LVHSGFF     +       Y LT +S+ L++         F + P L   LD 
Sbjct: 72  YRLMRILVHSGFFVQQNGSGNTVGEGYVLTNASQLLVKD------NPFSVTPLLLAELDP 131

Query: 127 ATIASMGALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFIGK 186
                   +S W     Q+ + S F  A+ +  W+Y++QEP     F++AM  D+  +  
Sbjct: 132 ILTEPWHHVSAWF----QNDDPSPFHTAHERTFWEYASQEPKLNQFFNEAMASDARLVSS 191

Query: 187 IVTEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNLSF 246
           ++  +C  VFEGL SLVDVGGGTG +A AIA  F H+ C+VFDLP VV++ + +  NL +
Sbjct: 192 VMINECKGVFEGLNSLVDVGGGTGTVAKAIATEFQHLDCTVFDLPHVVADLEGSG-NLKY 251

Query: 247 IGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNE 306
           +GGDM E +IP A+A++LKWILH+W+DE+ +KIL++CK+AI  R EGGK+I+IDMV++N 
Sbjct: 252 LGGDMFE-EIPQADAILLKWILHDWNDEECVKILRQCKEAIKGR-EGGKVIVIDMVVENN 311

Query: 307 VEDRESTETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLRTCGVGFRK 366
             +  STETQ FFD+LMM  + GKER + EW  LF +AGFS++ I   LG          
Sbjct: 312 EVEAGSTETQFFFDMLMMILVTGKERKKSEWSKLFSDAGFSNFKINPVLG---------- 371

Query: 367 PETNHNKMEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLV 426
               H        ELLQ+QAHVWNH     NSM+LKC+VELGIPD+IH H +PM+L+ LV
Sbjct: 372 --NLHGA------ELLQAQAHVWNHIFNFINSMSLKCSVELGIPDLIHNHGKPMTLAQLV 431

Query: 427 AALHIQPSKAQYLGRLMRPLVHSGFF----DADEQEDVRYSLTPSSRLLLRHTHTYTTFQ 486
           A L I P+K  ++ RLMR LVHSGFF     + + E   Y LT +S+  L+       F 
Sbjct: 432 AVLPIHPTKTHFVYRLMRILVHSGFFVQQNGSGDTEGEGYVLTNASQ--LQFLVKDNPFS 491

Query: 487 MIPFLCIALDKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQ 546
           + PFL   LD         +S+W     Q  + + F  A+ ++ WEYA+QEP     F +
Sbjct: 492 VTPFLQAMLDPILTQPWHHVSAWF----QNDDPSPFHTAHERTFWEYASQEPKLNQFFNE 551

Query: 547 IMVCDSELIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVS 606
            M  D+ L+  ++I +C GVFEGL SLVDVGGGTG +A AIA+ F H+ C+VFDLP VV+
Sbjct: 552 AMASDARLVSSVMINECKGVFEGLNSLVDVGGGTGTVAKAIAKEFQHLDCTVFDLPHVVA 611

Query: 607 NLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGK 666
           +L+ +  NL ++GGDM E +IP A+A++LKW+LH+W+DE+ ++IL++CK+AI  R EGGK
Sbjct: 612 DLEGS-GNLKYLGGDMFE-EIPQADAILLKWILHDWNDEECVQILRQCKEAIKGR-EGGK 671

Query: 667 LIIIDMVLDNEVEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKL 717
           +I+IDMV++N   +  STET LFFD+LMMV + GKERN+ EW  LF +AGFS++ I   L
Sbjct: 672 VIVIDMVVENNKREAASTETHLFFDMLMMVEVTGKERNKNEWSKLFSDAGFSNFKISPVL 698

BLAST of CmoCh20G006530.1 vs. NCBI nr
Match: XP_022944128.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 728.0 bits (1878), Expect = 7.8e-206
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0

Query: 361 MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP 420
           MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP
Sbjct: 1   MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP 60

Query: 421 SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDK 480
           SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDK
Sbjct: 61  SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDK 120

Query: 481 ATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGK 540
           ATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGK
Sbjct: 121 ATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGK 180

Query: 541 IVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSF 600
           IVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSF
Sbjct: 181 IVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSF 240

Query: 601 IGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLDNE 660
           IGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLDNE
Sbjct: 241 IGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLDNE 300

Query: 661 VEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVYP 720
           VEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVYP
Sbjct: 301 VEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVYP 359

BLAST of CmoCh20G006530.1 vs. NCBI nr
Match: KAG6570912.1 (putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 706.1 bits (1821), Expect = 3.2e-199
Identity = 348/359 (96.94%), Postives = 353/359 (98.33%), Query Frame = 0

Query: 361 MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP 420
           MEEK+DELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP
Sbjct: 1   MEEKLDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP 60

Query: 421 SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDK 480
           SKAQ+LGRLMR LVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDK
Sbjct: 61  SKAQHLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDK 120

Query: 481 ATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGK 540
           ATIASLGALSSWLCSSDQGHNSNSFEMANGK IWEYAAQEPNFGNLFQQIMVCDSELIGK
Sbjct: 121 ATIASLGALSSWLCSSDQGHNSNSFEMANGKPIWEYAAQEPNFGNLFQQIMVCDSELIGK 180

Query: 541 IVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSF 600
           IVIEKCS VFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNV+NLSF
Sbjct: 181 IVIEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVKNLSF 240

Query: 601 IGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLDNE 660
           IGGDMLESKIPPANAVMLKW+LHNWDDEQSIKILKKCKDAIPSR EGGKLIIIDMVLDNE
Sbjct: 241 IGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNE 300

Query: 661 VEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVYP 720
           VEDRESTETQLFFDVLMM NLNGKERNE+EWK LFMEAGFSDYTIICKLGLRSLIEVYP
Sbjct: 301 VEDRESTETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIICKLGLRSLIEVYP 359

BLAST of CmoCh20G006530.1 vs. NCBI nr
Match: XP_022944130.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 703.0 bits (1813), Expect = 2.7e-198
Identity = 345/345 (100.00%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   MEEKVDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMSIQPSKAQHLGRL 60
           MEEKVDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMSIQPSKAQHLGRL
Sbjct: 1   MEEKVDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMSIQPSKAQHLGRL 60

Query: 61  MRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSLDKATIASMG 120
           MRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSLDKATIASMG
Sbjct: 61  MRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSLDKATIASMG 120

Query: 121 ALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFIGKIVTEKCS 180
           ALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFIGKIVTEKCS
Sbjct: 121 ALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFIGKIVTEKCS 180

Query: 181 RVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNLSFIGGDMLE 240
           RVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNLSFIGGDMLE
Sbjct: 181 RVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNLSFIGGDMLE 240

Query: 241 SKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDREST 300
           SKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDREST
Sbjct: 241 SKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDNEVEDREST 300

Query: 301 ETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLR 346
           ETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLR
Sbjct: 301 ETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLR 345

BLAST of CmoCh20G006530.1 vs. NCBI nr
Match: KAG6570911.1 (putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 686.4 bits (1770), Expect = 2.6e-193
Identity = 339/355 (95.49%), Postives = 342/355 (96.34%), Query Frame = 0

Query: 1   MEEKVDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMS---------IQP 60
           MEEKVDE+LQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMS         IQP
Sbjct: 1   MEEKVDEVLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTHAQPMSLSDLVTALHIQP 60

Query: 61  SKAQHLGRLMRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSL 120
           SKAQHLGRLMRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSL
Sbjct: 61  SKAQHLGRLMRLLVHSGFFDANEQEDVKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSL 120

Query: 121 DKATIASMGALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFI 180
           DKATIASMGALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFI
Sbjct: 121 DKATIASMGALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFI 180

Query: 181 GKIVTEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNL 240
           GKIVTEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSN QP  KNL
Sbjct: 181 GKIVTEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVKNL 240

Query: 241 SFIGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLD 300
           SFIGGDMLESKIPPANAVMLKW+LHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLD
Sbjct: 241 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLD 300

Query: 301 NEVEDRESTETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVSKLGLRT 347
           NEVEDRESTETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIV KLGLR+
Sbjct: 301 NEVEDRESTETQLFFDVLMMANLNGKERNEREWKNLFMEAGFSDYTIVCKLGLRS 355

BLAST of CmoCh20G006530.1 vs. NCBI nr
Match: XP_022986324.1 (probable O-methyltransferase 3 [Cucurbita maxima])

HSP 1 Score: 652.5 bits (1682), Expect = 4.2e-183
Identity = 321/361 (88.92%), Postives = 338/361 (93.63%), Query Frame = 0

Query: 361 MEEKVDELLQSQAHVWNHALKLTNSMALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQP 420
           MEEK+DELLQSQAHVWNHALK  NSMALKCAVELGIPDVIH+HAQPM+LSDLVAAL IQP
Sbjct: 1   MEEKLDELLQSQAHVWNHALKFMNSMALKCAVELGIPDVIHSHAQPMTLSDLVAALRIQP 60

Query: 421 SKAQYLGRLMRPLVHSGFFDADEQEDVRYSLTPSSRLLLRH--THTYTTFQMIPFLCIAL 480
           SKAQYLGRLMR LVHSGFFDADEQEDVRYSLTPSSRLLLRH  TH++TTFQ+IPFL + L
Sbjct: 61  SKAQYLGRLMRLLVHSGFFDADEQEDVRYSLTPSSRLLLRHTDTHSHTTFQIIPFLYLGL 120

Query: 481 DKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELI 540
           DK TIASLGA S WLCSSDQ HNSNSFEMANGK IWEYAAQEP+FGNLFQQ MVCDSE+I
Sbjct: 121 DKTTIASLGAFSGWLCSSDQDHNSNSFEMANGKPIWEYAAQEPSFGNLFQQTMVCDSEMI 180

Query: 541 GKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNL 600
           GKIV EKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITC+VFDLPQVVSN Q   +NL
Sbjct: 181 GKIVTEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCTVFDLPQVVSNQQRTAKNL 240

Query: 601 SFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLD 660
            FIGGDMLE+KIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSR EGGKL+IIDMVLD
Sbjct: 241 RFIGGDMLEAKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRAEGGKLVIIDMVLD 300

Query: 661 NEVEDRESTETQLFFDVLMMVNLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEVY 720
           NE+E+RESTETQL FDVLMMVNL+GKERNEKEWK LFMEAGFSDYTI+CKLGLRSLIEVY
Sbjct: 301 NEMEERESTETQLLFDVLMMVNLHGKERNEKEWKNLFMEAGFSDYTIVCKLGLRSLIEVY 360

BLAST of CmoCh20G006530.1 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 191.0 bits (484), Expect = 3.2e-48
Identity = 111/353 (31.44%), Postives = 180/353 (50.99%), Query Frame = 0

Query: 5   VDELLQSQAHVWNHALKFFDSMSLRCAVELGIPDVIHTH--AQPMSIQ------PSKAQH 64
           +DE  ++   +W +   F D  + +CA++L IP+ I  H  +QP+++        +   H
Sbjct: 23  LDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSH 82

Query: 65  LGRLMRLLVHSGFFDANEQED---VKYSLTPSSRRLLRHTHTHTRTTFQIIPFLFLSLDK 124
           L R+MR LVH G F     +D     Y  TP SRRL+       R    + PF+      
Sbjct: 83  LRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLM----ITRRDGKSLAPFVLFETTP 142

Query: 125 ATIASMGALSGWLCSSDQDHNSSSFEMANGKPMWDYSAQEPSFGNLFHQAMVCDSEFIGK 184
             +A    LS  + S         F+  +GK +W ++   P   ++ ++AM CD+  +  
Sbjct: 143 EMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVVP 202

Query: 185 IVTEKCSRVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNQQPTAKNLSF 244
            V   C  +F+G+ ++VDVGGGTG     + + FP I    FDLP V+   +     +  
Sbjct: 203 RVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAE-VLDGVEN 262

Query: 245 IGGDMLESKIPPANAVMLKWILHNWDDEQSIKILKKCKDAIPSRAEGGKLIIIDMVLDN- 304
           + GDM +S IP  +A+ +KW+LH+W D+  IKILK CK+A+P     GK++I++ V+   
Sbjct: 263 VEGDMFDS-IPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNI--GKVLIVESVIGEN 322

Query: 305 ------EVEDRESTETQLFFDVLMMANLN-GKERNEREWKNLFMEAGFSDYTI 339
                 +  D +    +L  D++MMA+ + GKER  +EW  +  EAGF+ Y +
Sbjct: 323 KKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEV 367

BLAST of CmoCh20G006530.1 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 175.3 bits (443), Expect = 1.8e-43
Identity = 119/375 (31.73%), Postives = 181/375 (48.27%), Query Frame = 0

Query: 361 MEEKVDELLQSQA----HVWNHALKLTNSMALKCAVELGIPDVIHTH--AQPMSLSDLVA 420
           MEE    LL  +A     +W +     +  A KCA++L IP+ I  H  +QP++LS+L +
Sbjct: 1   MEESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSS 60

Query: 421 ALHIQPSKAQYLGRLMRPLVHSGFFDADEQED---VRYSLTPSSRLLLRHTHTYTTFQMI 480
           A+   PS   +L R+MR LVH G F     +D     Y+ TP SR ++            
Sbjct: 61  AVSASPS---HLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM------------ 120

Query: 481 PFLCIALDKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWEYAAQEPNFGNLFQQIM 540
                                                +GK +W +A        L  + M
Sbjct: 121 ---------------------------------ITKLHGKDLWAFAQDNLCHSQLINEAM 180

Query: 541 VCDSELIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNL 600
            CD+  +   V   C G+F+G+ ++VDVGGGTG     + + FP I    FDLP V+   
Sbjct: 181 ACDARRVVPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVA 240

Query: 601 QPNVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLI 660
           Q  +  +  + GDM +S IP ++AV++KWVLH+W D+  IKILK CK+A+      GK++
Sbjct: 241 QV-LDGVENVEGDMFDS-IPASDAVIIKWVLHDWGDKDCIKILKNCKEAV--LPNIGKVL 300

Query: 661 IIDMVLDN-------EVEDRESTETQLFFDVLMMVNLN-GKERNEKEWKYLFMEAGFSDY 719
           I++ V+         E  D +    +L  D++MMV+ + GKER  KEW ++  EAGF+ Y
Sbjct: 301 IVECVIGEKKNTMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARY 323

BLAST of CmoCh20G006530.1 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 154.8 bits (390), Expect = 2.6e-37
Identity = 103/326 (31.60%), Postives = 169/326 (51.84%), Query Frame = 0

Query: 402 THAQPMSLSDLVAALHIQPSKAQYLGRLMRPLVHSGFF------DADEQEDVRYSLTPSS 461
           ++  P+ L+ + A  +  P     + RL+R LV           D + +E   Y L    
Sbjct: 52  SYLSPVDLASMAAPKN--PHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVG 111

Query: 462 RLLLRHTHTYTTFQMIPFLCIALDKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWE 521
           + L++       F + P++     KA     G + S+L  + Q   ++++E AN   I+E
Sbjct: 112 KKLIKDE---DGFSIAPYVLAGCTKAK----GGVWSYLTEAIQEGGASAWERANEALIFE 171

Query: 522 YAAQEPNFGNLFQQIMVCDSELIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFP 581
           Y  +  N   +F + M   + ++ K ++E   G FEG+   VDVGG  G     I   +P
Sbjct: 172 YMKKNENLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYP 231

Query: 582 HITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILK 641
           HI    FDLP +V    P +  +  IGGDM + +IP    +++KW+LH+W+DE+ ++ILK
Sbjct: 232 HIKGINFDLPHIVKE-APQIHGVEHIGGDMFD-EIPRGEVILMKWILHDWNDEKCVEILK 291

Query: 642 KCKDAIPSRGEGGKLIIIDMVLDNEVEDRE-STETQLFFDVLMM-VNLNGKERNEKEWKY 701
            CK A+P   E G++I+I+M++  EV + + +T+  L  D+ MM +   GKER +KE++ 
Sbjct: 292 NCKKALP---ETGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFED 351

Query: 702 LFMEAGFSDYTIICKLGLRSLIEVYP 720
           L  EAGF    II       +IE+YP
Sbjct: 352 LAKEAGFKLPKIIYGAYSYWIIELYP 362

BLAST of CmoCh20G006530.1 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 150.6 bits (379), Expect = 4.8e-36
Identity = 103/341 (30.21%), Postives = 167/341 (48.97%), Query Frame = 0

Query: 386 MALKCAVELGIPDVIHTHAQPMSLSDLVAALHIQ-PSKAQYLGRLMRPLVHSGFFDADEQ 445
           MALK A+EL + +++  +  PMS +++ + L  + P     L R++R L          +
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNR 93

Query: 446 E------DVRYSLTPSSRLLLRHTHTYTTFQMIPFLCIALDKATIASLGALSSWLCSSDQ 505
           +      +  Y L P  + L ++    +    I  LC+      +     + SW    D 
Sbjct: 94  KLSGDGVERIYGLGPVCKYLTKNEDGVS----IAALCLMNQDKVL-----MESWYHLKDA 153

Query: 506 GHNSN-SFEMANGKSIWEYAAQEPNFGNLFQQIMVCDSELIGKIVIEKCSGVFEGLKSLV 565
             +    F  A G S +EY   +P F  +F   M   S +  K ++E   G FEGL SLV
Sbjct: 154 ILDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLV 213

Query: 566 DVGGGTGVMANAIAEAFPHITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVM 625
           DVGGG G     I   +P++    FDLP V+ +  P+   +  +GGDM  S +P  +A+ 
Sbjct: 214 DVGGGIGATLKMIVSKYPNLKGINFDLPHVIED-APSHPGIEHVGGDMFVS-VPKGDAIF 273

Query: 626 LKWVLHNWDDEQSIKILKKCKDAIPSRGEGGKLIIIDMVLDNEVEDRESTETQLFFDVLM 685
           +KW+ H+W DE  +K LK C +++P   E GK+I+ + +L    +   ST+  +  D +M
Sbjct: 274 MKWICHDWSDEHCVKFLKNCYESLP---EDGKVILAECILPETPDSSLSTKQVVHVDCIM 333

Query: 686 MV-NLNGKERNEKEWKYLFMEAGFSDYTIICKLGLRSLIEV 718
           +  N  GKER EKE++ L   +GF    ++C     +LIE+
Sbjct: 334 LAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIEL 359

BLAST of CmoCh20G006530.1 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 147.9 bits (372), Expect = 3.1e-35
Identity = 101/326 (30.98%), Postives = 167/326 (51.23%), Query Frame = 0

Query: 402 THAQPMSLSDLVAALHIQPSKAQYLGRLMRPLVHSGFF------DADEQEDVRYSLTPSS 461
           ++  P+ L+ + A  +  P     + RL+R LV           D + +E   Y L    
Sbjct: 52  SYLSPVDLASMAAPKN--PHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVG 111

Query: 462 RLLLRHTHTYTTFQMIPFLCIALDKATIASLGALSSWLCSSDQGHNSNSFEMANGKSIWE 521
           + L++       F + P++     KA     G +   +  + Q   ++++E AN   I+E
Sbjct: 112 KKLIKDE---DGFSIAPYVLAGCTKAK----GGVWYNVQHAIQEGGASAWERANEALIFE 171

Query: 522 YAAQEPNFGNLFQQIMVCDSELIGKIVIEKCSGVFEGLKSLVDVGGGTGVMANAIAEAFP 581
           Y  +  N   +F + M   + ++ K ++E   G FEG+   VDVGG  G     I   +P
Sbjct: 172 YMKKNENLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYP 231

Query: 582 HITCSVFDLPQVVSNLQPNVRNLSFIGGDMLESKIPPANAVMLKWVLHNWDDEQSIKILK 641
           HI    FDLP +V    P +  +  IGGDM + +IP    +++KW+LH+W+DE+ ++ILK
Sbjct: 232 HIKGINFDLPHIVKE-APQIHGVEHIGGDMFD-EIPRGEVILMKWILHDWNDEKCVEILK 291

Query: 642 KCKDAIPSRGEGGKLIIIDMVLDNEVEDRE-STETQLFFDVLMM-VNLNGKERNEKEWKY 701
            CK A+P   E G++I+I+M++  EV + + +T+  L  D+ MM +   GKER +KE++ 
Sbjct: 292 NCKKALP---ETGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFED 351

Query: 702 LFMEAGFSDYTIICKLGLRSLIEVYP 720
           L  EAGF    II       +IE+YP
Sbjct: 352 LAKEAGFKLPKIIYGAYSYWIIELYP 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B0ZB571.1e-9651.09Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1[more]
B6VJS41.4e-9349.30Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1... [more]
A0A0N9HMN64.8e-8947.97Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P... [more]
Q6VMW05.5e-8546.038-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=... [more]
Q8GSN17.1e-8547.37Myricetin O-methyltransferase OS=Catharanthus roseus OX=4058 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FXQ63.8e-206100.00probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111448676 PE=... [more]
A0A6J1FYH41.3e-198100.00probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111448677 PE=... [more]
A0A6J1JFR92.0e-18388.92probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111484102 PE=3 ... [more]
A0A6N2MMG05.9e-18348.25Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS385670 PE=3 SV=... [more]
A0A6N2LSG28.8e-17946.35Uncharacterized protein (Fragment) OS=Salix viminalis OX=40686 GN=SVIM_LOCUS2715... [more]
Match NameE-valueIdentityDescription
XP_022944128.17.8e-206100.00probable O-methyltransferase 3 [Cucurbita moschata][more]
KAG6570912.13.2e-19996.94putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022944130.12.7e-198100.00probable O-methyltransferase 3 [Cucurbita moschata][more]
KAG6570911.12.6e-19395.49putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022986324.14.2e-18388.92probable O-methyltransferase 3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G35160.13.2e-4831.44O-methyltransferase family protein [more]
AT4G35150.11.8e-4331.73O-methyltransferase family protein [more]
AT1G51990.12.6e-3731.60O-methyltransferase family protein [more]
AT5G54160.14.8e-3630.21O-methyltransferase 1 [more]
AT1G51990.23.1e-3530.98O-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 357..458
e-value: 5.9E-39
score: 133.8
coord: 1..89
e-value: 7.8E-29
score: 101.3
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 465..719
e-value: 2.2E-77
score: 261.6
coord: 98..346
e-value: 4.9E-75
score: 253.9
NoneNo IPR availablePIRSRPIRSR005739-1PIRSR005739-1coord: 25..344
e-value: 6.0E-80
score: 266.3
coord: 385..718
e-value: 5.5E-86
score: 286.1
NoneNo IPR availablePANTHERPTHR11746:SF250O-METHYLTRANSFERASEcoord: 364..719
NoneNo IPR availablePANTHERPTHR11746:SF250O-METHYLTRANSFERASEcoord: 5..345
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 556..657
e-value: 0.00125199
score: 37.0243
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 133..334
e-value: 1.9E-54
score: 184.2
coord: 499..701
e-value: 8.0E-56
score: 188.7
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 26..65
e-value: 3.4E-8
score: 33.4
coord: 386..434
e-value: 8.3E-17
score: 60.9
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 5..345
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 364..719
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 376..719
score: 76.83947
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 16..353
score: 67.912781
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 103..343
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 469..718
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 365..461
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 5..92

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh20G006530CmoCh20G006530gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh20G006530.1:exon:1836CmoCh20G006530.1:exon:1836exon
CmoCh20G006530.1:exon:1837CmoCh20G006530.1:exon:1837exon
CmoCh20G006530.1:exon:1838CmoCh20G006530.1:exon:1838exon
CmoCh20G006530.1:exon:1839CmoCh20G006530.1:exon:1839exon
CmoCh20G006530.1:exon:1840CmoCh20G006530.1:exon:1840exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh20G006530.1:cdsCmoCh20G006530.1:cdsCDS
CmoCh20G006530.1:cdsCmoCh20G006530.1:cds_2CDS
CmoCh20G006530.1:cdsCmoCh20G006530.1:cds_3CDS
CmoCh20G006530.1:cdsCmoCh20G006530.1:cds_4CDS
CmoCh20G006530.1:cdsCmoCh20G006530.1:cds_5CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh20G006530.1:three_prime_utrCmoCh20G006530.1:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh20G006530.1CmoCh20G006530.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity