CmoCh20G005910.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G005910.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionDiacylglycerol kinase
LocationCmo_Chr20: 2899368 .. 2906415 (-)
Sequence length1257
RNA-Seq ExpressionCmoCh20G005910.1
SyntenyCmoCh20G005910.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTAGCTATCCGAAACAGGTAATGGCGACGCGCCTTTCTGAATCTTCCCTTCCCTCCACTTTCTCCGTTTATATATATATATAGTATGTTTTTCAGCCCACTGGGGAGAGAGGTGATTAAGATTTCTGTAATTCAGTTCCGTCAACGCTTTGGTTTTACGGTGGCTTTTCCGGGTTAGTTCGAACTTTTTTATTTGCTCCGCAGCCTTTTCTCTGATTCTCTTTTCGGAGATTTCTATATTGTGTTTTTTTTTTTTTTTTTTTTTGCTTCTTCTGGTATTTTACGGGCATTTCTAGGCGCCGACGGGGAAGATAGAGAATCAGAATAGTAGAATTTTTCTGTTTCGTTGATCTGTTGCTTCTCTGCAATTGTTTAACTCTGGAATTTGAATTTCCACCTGATTTTTTTTCCTTGGGGGGAGGGGGGAGAGGGGGCATAATGATCTTTACGGATCAGAGATATTCCTTGCGCTTTTTTATGCTTTAACGTCTTGAATATTCTGAATTTTGTCTGTTTCGCTTTTCGAATGGGCCATTTTCCCACTCTTAAAGATTCATTGCTGCCGAGCTGAGACTTTTCGTGTCATTTGTGTGTAATGTGAATATCTTGCACAATTATGGACATTTAGCATTCGTAATCTATAATGTCGTAATCGTATATGTTGACTTGCAATTTTTGTGCTTAATTGGCAATTCATCACGGGGATTTCTCTGAAAGCGTTACCCTTGTGAATTTTTATTTTATTTTTAGAGGTCTGAAATCGGTTAAGAATCTATACCTAACCTGCTACTCATGATTCATTGCATTTGATATTGAAGATTTGTTGAGTACTGATCAACTGATCACAAGAATTGGCATCTATAATCTTTATTTGGGGACTGAATAAGTGATGGGATGAAGTTTCATTTCTTTTATTTTCTGTATGTTTTTGTTCCCAAATGATTCATGCTACTAGTGTAGAATGGTATTATCTTGAGAAACCTTTGGTGTTCACATAACCTACTAATTCCAAAAATTTTGCAATTCATTGATTTATGTGATTTAAGGATATCTATTTTGCTTTGTTGTCTGCATAAATCAGACTCATGATACCCATTTTACAATGAGTATAAATTTATGAACACTTTCCCAATGTTTTTTTTAATGATGATGCAAATCGTGCAATGATGCAGTTCGAAGCAATGGCGACAAATTTCCCATTTGATTCCGAATTCTTTAAGAATTTTCTCATCCCTGATTACATACTCCGACCTGAAGCTGAAGCTAAAGTTGAGAGCGTTCCTTATGTTCCCAATTGTCCTGTCTTAGTATTTATCAACTCCAGAAGTGGTGGTCAGCTTGGTGGCAGTCTCCTTAGCACATATCGTTCTCTTCTCAATGAGAAACAGGTGATTTTTTGGTTACAAAGGTTTTAGATGTACTTTGTCGGCCTCTGGGAGCTATATAGGAAATTACTGAATTTACTGTTGGTTGACTTCTATCTGACTAATTGTAAGGTTTTTGATTTGGGGGAAGAAGCTCCTGACGTGGTGCTGCGCAGATTCTTCTTGAACCTTGAAAAGCTTAAGCTTAATGGCGATGAAATTGCAGTCGAAATTCAGAAGAGATTGCGACTAATAGTGAGTTTCTGATGTGCTTTCCAATATTTACGCCAAGTTCAAAATTCTTTGGTTTAGCAATATACACTGTTAAATTCCATCCTCAATGCATTTGATCTTTCAGGTTGCAGGGGGTGATGGAACAGCTGGCTGGCTTCTTGGAGTTGTTTGCGATCTTAAGTTATCTTATTCACCTCCAATTGCTACGGTGCCCTTAGGAACTGGAAACAATCTTCCTTTTGCATTTGGTTGGGTAATTTTTTTAAAATTATTTCACACTTTCTGGTTACCATATTAACATGGTAGCATTGCAAAGGATGTTGAGCAAATATAATTGAAAACTGAGTTCATATATTTGATTGCTGCTTTATTATTGATTAGAATGTTGGTATTGTCGAAAACCTTGTTGATAGGCATTTGAAGGATGAATGCTTTTCTGTGAAAACTGTGCATTTTAGTTTGATATCAGCCACTGACATCGATATAATGTTTAATATCCTCCTGATATTATTATCAGGCAGCACAAGAATTGAAACAAAAATATTTTCCTAATTTCAGGGAAAGAGGAATCCTGGAACAGACCTTAACGCTGTGATTTCATTTTTAGATCAAGTCCTGAAAGCAAAGGAAATGAAAATTGACAAGTAAGAAAACAGGATAGAAAGGCTGACACACACATGTTTGCATATGTATGTGCTCATCATAAACTCAACATTATCATAATCAAAAGCTGTAATGGATCTTGTTATTGTTGTATGACAGTTGGCATATTCTGATGAGGATGAGAGCTCCAGCAGAAGGCTCATGTGATCCTATTGCTCCTCTTGAATTGCCACATTCCCTGCATGCATTTCATCGGGTCACCGAGGGTGATGAACACAAGGTGGTAACTACATACTATGTGGAACACATTCCCTATTTGTATAAGATTTTGATATAAAAGAGTATTTTGATGTTCTAAATAAATCTTAGTCGATTATATTGTTTTCTCTCAAAGAAACCTGCATGCGAAGCAACTCATCACCATCTTAAGTAGTTACTTACCGGGTTAATTTATCCGCCTGCCTCTTATCTGAAAAAATTTTAGCCGCCTATTTCAACACCTGCAAAACTACATGGTCATACTTGAGGTTGAAGTTAAGTTGTTCAAAAGGTACTTTATAGCTGAGTTAGCAATTCAGAACACAAGACAGGAGCAATGATGTATTTTGATGGAACTTATAGAACAATAGTATTGGATAAGTGGTGGCTAGATTTCACTCTGTTCAAGTATGTTATTTTGGAACTAGTCTTGAATAGAGTTCTTATTTATGAAGAACTTAGATTACAGAAGACTTGATAAATACTCGCTGTATGTCCTTGGAAATGAGCAGTTTTAACTGAACCATGAAGTGTGATTGAAAGATACAAGTGATAAATATAAATTAGAAACCAAAGTCATCCTCTCTTGATTATACTTCATAAGTATTTAGCTCATTTTCAATTATAAGTTCCATTCATTTTTTTTTTAAGCAATATTCTTAACGTAGGCTCTGAAACTATATAAAGATTATGCACTCAACTCAACATGTAGAAAAAGTATTATTTGTGGAAAGAAGGGCTCAAGTTCTCTTTTTTCTTTTTTTTAATCTCATTTTATGGAAGAAATTTCACCTTCAGTTATGTTTTTTATAAGTCAATATTCTTCTCTCTTTATGTGCTGAACTACTTACTTGATTCTCAGGAAGGTTGCCTCACATTCCGTGGGGGATTTTGGAACTACTTCAGCATGGGTAAGACTACTTCCATGACAGTTCTAGATAAAAGTTCAACATGTTACTGAGATTTGTAATACATCACAGGAGATATGTCCTAAATCATTTCCGGAAAGAAAAATCCTTATTTTGATGATCTCTTAATGGGAAATTTATTCGACATTCGGCTTCTACTGTTTGCAATTTTGTATACTGTTAATTTCCAATTTAGTTTGCAATTACTGGTAATGGTCTCTGACATGAGAGTTGTTTCTTCGTTTTGATTGCAGGAATGGACGCTCAAGTATCATATGCTTTTCATTCTGAGAGAAAGATGCATCCTGAAAAATTTAAGAATCAGTTAGTTAATCAGGTAAGTGTTACAAATGTCTTCATGAATTTGCTTGCAGACTAGTTTCCAATCATACTAAAATCTTTATATGAGATAATAGATACTATGAACGCAATTGATTTTTGAAACATTGAACATCAAAATATGAACTTCAACGTGTAAGCTTTCTTGGATATAATATGTCAACCACATTTAACTCTTTGATTATTAGTGTATTGCTCATATACCAAAATTTAACCTTTTTCTCCTCATTTGTGTACCAGAGTACCTATGCAAAGATTGGCAGCACCTGGTTTTTTGCTCCTTTGTTTCATCCTTCATCCATGTGCGTACTCAAATGATTTTATTTATTTATTTTGTGCGGGGGGGAGGGGGTACTTCATAGTGAAAAGTAGTAACCTAAATTACCAACCGTGATGAGGTACACAAAATTTCCAATACATCCCTCATGAGGTATTCAACAACCCCCGTAATGTTTCGCCTCCCCATTGGGAGACTTAGCAAAATGCACTGAAACATCTTGCCAAATCCCAGCTCTCATCATTAACCACTCCCTCATTCGATTTCATTATTTCCAGAAACTCCAATGAGTTGTTTTAGCTTGATCCATAATATTTTAGCCTTCTTCTGTTCTATGCCGCTTAACATCTGTATTAAGTTTCTCCTTTGCTGCTATGTGGAGATCCCAAGATTGGTTGAAACTCACATTTAAATGCCTAAAACGTGCTACTGTATTCTGTACTGTCAATGAAAGAATAGGCGGTCTTCATTTTCCTTTTGCTTCTTACTGATTTCAGCAACGAGGGTTGAAAAAACATGTTAATGCATCTATTCATCGTAATGACTCCCCCCATTGAACAGCGTCCACACATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCACTTTTTTTGCTCCTTGTAATCACCACAGAATGGGTTACCCTGTCTTTTAACTCTGGTAGTCACCATAGAATGATCTTTAACAAGTTCAGAGCAATTGCGCCACAACTCTGATTACAATGGAAAAGGCCTTCAAATTTTAATGTAACAAATGGGTATTTCTGCTTATTTTTTCAAAAGAGAATACAAGAATGATATGTTTCATTAACCTTCAGTTTGTTCCCTTTTCTTTGTTTAACTAGTTTCCTGTCACTATATAGGAATATATCTCAAATTGCTAAGGTCGAGATCATGAAACATCATGGTGATTGGCAAACTTTGCCTATACCTCCTGGGTAAAGTTTTTCAGCTTTCAAAGTTACTTACTGGACCCAATTCTACTTTCTTCACATAACTTCTGTTTTGGAACGATATTAAAAAAATTGTTCAAATTTCAGATAATGTCTTTGTCTGTTTGCACTAATCTGATTTTCTTTTGAACTGCAATGTACTCAATTACCATTTTTCTCGAATGACATGCCCTTTTCTCATTTTTACCTATCTGATGCCTATAATATATTTATCCTTCCAATTTTTTGCTTAGCATGCAGAAGAACAAGTATGGAAGTGTTGATGCATCTCATTTATTTTAGTATTGCATGTCTTATTGTGAATACTACTTCTATTGTTTTAAAATTATAATTTGAAACTGAAACCAGCAAGCACCGTACATCCTACTTTTTTCTTCTTAATTAGTACTGTATTACTCGATTTGTGTCAATATGGTCTTGCTAATTATTCTCGCAAGAAGATAGTGTACCCCTCCTCTGTCACCTTGGTTAATGATAATGGTTAAGATGCTGTCTGATAACCATTTTGTTTTCTAATTTTGGAAGTCAATCTTATACGCATTATTTTAACCTAGAGTTTCTTTTGGTCACATGTTCTTTAAACCAAAATTGGAAAACTAAAAAAAGTAGTTTTCAAAATTTGTTTTCATTCTTTTAAATTTGGCTCAGAGTTTAAATGTGTTCTTTAAAAAGTGGAAAGATTACCAAAGAATTTGTGTGGGGAGAGCAAGTATAATTGTTAGAAACAAAAAAACGAAAAATGAAATTGTGGTCAAACGTTCAGTAGTGTCTAGGACCCTTTCCTTATGATACCGTGTGCTTCTCTTTCTTATTACGAAATGCTGTTCCCTGATGTTTGCTACAGAATCAGGTCAATCGTATGCCTTAACTTGCCCAGCTTTTCCGGTGGATTCAATCCATGGGGTAAACCAAATTGCAGGAGGCAGCGTGATGTTAGTTCCCGATCTATTTATTTTCTATTTAGATTTGAAGTAATCTGAATCCAGATGTTGGTGCAATGCTTTAATTACTTTCACGTGTTTCTCTTTTAGTTCCTAATGTCATTCCTTCATGAAATGTGGTTTATAGAGAGACTTTACTCCACCATACGTCGATGATGGCCTTCTTGAGGTTGTGGGATTTCGGGATGCTTGGCATGGACTCGTTTTGCTTGCTCCCAGTGGACATGGAACTCGTCTTGCGCAGGTGAGAAAATTTAATCAAGGACTGAGGACACAAGAGGGTCTCTAATCTATATTAGCTAGTCTTAGTTTAGGTGGTAACCTAAGCACAACGAATTGACTAAGACATATATACTTTCTACTGAGAGTTAAAAGGTGAAGAGATCTGACTAAGACATATATACTTTCGACTGAGAGTTAAAAGGTATGAATCTCTTCACCATAAAAAAAAATCTTTAGTCTTTTTGGTTAAAAATGGTTGAACATAAAGTTGGTATATTAAAGTAGACATTATGGTAAAGCCTAACTTCATTATATAAGTCCAATGAAGGCTTTTGAAAGCAGATAAAGATTCGTTTATTTCCATGTGTTACAGTTGTGGATCATAACCTTCAGCATATGGGATATAGGTTATGGTAAAATGCTCATAACAGCCAAAACTATTATATTTATGTTTATTTTCATAGTAATGAGGTATTGTTCTGAATTTGTCTGTGTCTCTGTTGTATTTGCTCACACTTCGCCTCTGATTTGGTGCAAGGCTCATCGCATCAGGTTCGAGTTCCAGAAAGGTAAAGCAGATCACACATTCATGAGAATTGATGGGGAACCCTGGAAGCAACCCCTCCCTGCCAAAGAATCTGTTATGGTGGAAATCTCTCATCTTGGTCAAGTCAACATGCTCGCCACCCATAGTTGCATATCAAGAAGTGTTAATGATCCCTCAACACCCAGCAGGATTGATGAGGATTCAGAAGAGGAGGATTGTGAAGATGACTCCACAAAAGGTGGAGAAGAATTCAGGAAATTTGGTGCAGCAGCCACCTTTAAGATTCCAGATGAAGTTGATATATCTCAACTTAGTTGA

mRNA sequence

ATGTGTAGCTATCCGAAACAGTTCGAAGCAATGGCGACAAATTTCCCATTTGATTCCGAATTCTTTAAGAATTTTCTCATCCCTGATTACATACTCCGACCTGAAGCTGAAGCTAAAGTTGAGAGCGTTCCTTATGTTCCCAATTGTCCTGTCTTAGTATTTATCAACTCCAGAAGTGGTGGTCAGCTTGGTGGCAGTCTCCTTAGCACATATCGTTCTCTTCTCAATGAGAAACAGGTTTTTGATTTGGGGGAAGAAGCTCCTGACGTGGTGCTGCGCAGATTCTTCTTGAACCTTGAAAAGCTTAAGCTTAATGGCGATGAAATTGCAGTCGAAATTCAGAAGAGATTGCGACTAATAGTTGCAGGGGGTGATGGAACAGCTGGCTGGCTTCTTGGAGTTGTTTGCGATCTTAAGTTATCTTATTCACCTCCAATTGCTACGGTGCCCTTAGGAACTGGAAACAATCTTCCTTTTGCATTTGGTTGGGGAAAGAGGAATCCTGGAACAGACCTTAACGCTGTGATTTCATTTTTAGATCAAGTCCTGAAAGCAAAGGAAATGAAAATTGACAATTGGCATATTCTGATGAGGATGAGAGCTCCAGCAGAAGGCTCATGTGATCCTATTGCTCCTCTTGAATTGCCACATTCCCTGCATGCATTTCATCGGGTCACCGAGGGTGATGAACACAAGGTGAGAGACTTTACTCCACCATACGTCGATGATGGCCTTCTTGAGGTTGTGGGATTTCGGGATGCTTGGCATGGACTCGTTTTGCTTGCTCCCAGTGGACATGGAACTCGTCTTGCGCAGTTGTGGATCATAACCTTCAGCATATGGGATATAGGTTATGTAATGAGGTATTGTTCTGAATTTGTCTGTGTCTCTGTTGTATTTGCTCACACTTCGCCTCTGATTTGGTGCAAGGCTCATCGCATCAGGTTCGAGTTCCAGAAAGGTAAAGCAGATCACACATTCATGAGAATTGATGGGGAACCCTGGAAGCAACCCCTCCCTGCCAAAGAATCTGTTATGGTGGAAATCTCTCATCTTGGTCAAGTCAACATGCTCGCCACCCATAGTTGCATATCAAGAAGTGTTAATGATCCCTCAACACCCAGCAGGATTGATGAGGATTCAGAAGAGGAGGATTGTGAAGATGACTCCACAAAAGGTGGAGAAGAATTCAGGAAATTTGGTGCAGCAGCCACCTTTAAGATTCCAGATGAAGTTGATATATCTCAACTTAGTTGA

Coding sequence (CDS)

ATGTGTAGCTATCCGAAACAGTTCGAAGCAATGGCGACAAATTTCCCATTTGATTCCGAATTCTTTAAGAATTTTCTCATCCCTGATTACATACTCCGACCTGAAGCTGAAGCTAAAGTTGAGAGCGTTCCTTATGTTCCCAATTGTCCTGTCTTAGTATTTATCAACTCCAGAAGTGGTGGTCAGCTTGGTGGCAGTCTCCTTAGCACATATCGTTCTCTTCTCAATGAGAAACAGGTTTTTGATTTGGGGGAAGAAGCTCCTGACGTGGTGCTGCGCAGATTCTTCTTGAACCTTGAAAAGCTTAAGCTTAATGGCGATGAAATTGCAGTCGAAATTCAGAAGAGATTGCGACTAATAGTTGCAGGGGGTGATGGAACAGCTGGCTGGCTTCTTGGAGTTGTTTGCGATCTTAAGTTATCTTATTCACCTCCAATTGCTACGGTGCCCTTAGGAACTGGAAACAATCTTCCTTTTGCATTTGGTTGGGGAAAGAGGAATCCTGGAACAGACCTTAACGCTGTGATTTCATTTTTAGATCAAGTCCTGAAAGCAAAGGAAATGAAAATTGACAATTGGCATATTCTGATGAGGATGAGAGCTCCAGCAGAAGGCTCATGTGATCCTATTGCTCCTCTTGAATTGCCACATTCCCTGCATGCATTTCATCGGGTCACCGAGGGTGATGAACACAAGGTGAGAGACTTTACTCCACCATACGTCGATGATGGCCTTCTTGAGGTTGTGGGATTTCGGGATGCTTGGCATGGACTCGTTTTGCTTGCTCCCAGTGGACATGGAACTCGTCTTGCGCAGTTGTGGATCATAACCTTCAGCATATGGGATATAGGTTATGTAATGAGGTATTGTTCTGAATTTGTCTGTGTCTCTGTTGTATTTGCTCACACTTCGCCTCTGATTTGGTGCAAGGCTCATCGCATCAGGTTCGAGTTCCAGAAAGGTAAAGCAGATCACACATTCATGAGAATTGATGGGGAACCCTGGAAGCAACCCCTCCCTGCCAAAGAATCTGTTATGGTGGAAATCTCTCATCTTGGTCAAGTCAACATGCTCGCCACCCATAGTTGCATATCAAGAAGTGTTAATGATCCCTCAACACCCAGCAGGATTGATGAGGATTCAGAAGAGGAGGATTGTGAAGATGACTCCACAAAAGGTGGAGAAGAATTCAGGAAATTTGGTGCAGCAGCCACCTTTAAGATTCCAGATGAAGTTGATATATCTCAACTTAGTTGA

Protein sequence

MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKVRDFTPPYVDDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIWCKAHRIRFEFQKGKADHTFMRIDGEPWKQPLPAKESVMVEISHLGQVNMLATHSCISRSVNDPSTPSRIDEDSEEEDCEDDSTKGGEEFRKFGAAATFKIPDEVDISQLS
Homology
BLAST of CmoCh20G005910.1 vs. ExPASy Swiss-Prot
Match: Q9C5E5 (Diacylglycerol kinase 5 OS=Arabidopsis thaliana OX=3702 GN=DGK5 PE=2 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 4.0e-128
Identity = 265/518 (51.16%), Postives = 296/518 (57.14%), Query Frame = 0

Query: 19  SEFFKNFLIPDYIL--RPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLN 78
           S+F K F IP Y+L    E E + ES P  P  PVLVFINS+SGGQLGG L+ TYRSLLN
Sbjct: 8   SDFLKEFYIPTYVLSAETEEEEEEESRP-TPASPVLVFINSKSGGQLGGELILTYRSLLN 67

Query: 79  EKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVC 138
             QVFDL +E PD VLRR +LNLE+LK   D+ A +I+++L++IVAGGDGTAGWLLGVVC
Sbjct: 68  HNQVFDLDQETPDKVLRRIYLNLERLK--DDDFARQIREKLKIIVAGGDGTAGWLLGVVC 127

Query: 139 DLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHIL 198
           DLKLS+ PPIATVPLGTGNNLPFAFGWGK+NPGTD  AV SFL+QVLKAK MKIDNWHIL
Sbjct: 128 DLKLSHPPPIATVPLGTGNNLPFAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHIL 187

Query: 199 MRMRAPAE-GSCDPIAPLELPHSLHAFHRVTEGDE------------------------- 258
           MRM+ P E GSCDP+APLELPHSLHAFHRV+  DE                         
Sbjct: 188 MRMKTPKEGGSCDPVAPLELPHSLHAFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQI 247

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 248 SYAFHSERKLHPEKFKNQLVNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNG 307

Query: 319 --------HKVRD----------------------------FTPPYVDDGLLEVVGFRDA 378
                   H +R                              TPP+VDDGL+EVVGFR+A
Sbjct: 308 QWQDLHIPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNA 367

Query: 379 WHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIWCKAHRI 412
           WHGLVLLAP+GHGTRLAQ                                      A+RI
Sbjct: 368 WHGLVLLAPNGHGTRLAQ--------------------------------------ANRI 427

BLAST of CmoCh20G005910.1 vs. ExPASy Swiss-Prot
Match: Q7XQT2 (Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 436.8 bits (1122), Expect = 2.8e-121
Identity = 251/519 (48.36%), Postives = 289/519 (55.68%), Query Frame = 0

Query: 24  NFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLNEKQVFDL 83
           ++ IPDYIL P++E  +  V   P CPV+VFINSRSGGQLG SL+ TYR LLN+ QVFDL
Sbjct: 21  DYCIPDYILNPDSEQVL--VDQAPCCPVVVFINSRSGGQLGSSLIKTYRELLNKAQVFDL 80

Query: 84  GEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVCDLKLSYS 143
            EEAP+ VL R + N EKLK NGD IA +IQ  LRLIVAGGDGTA WLLGVV DLKLS+ 
Sbjct: 81  SEEAPEKVLHRLYCNFEKLKSNGDPIAFQIQSNLRLIVAGGDGTASWLLGVVSDLKLSHP 140

Query: 144 PPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHILMRMRAPA 203
           PPIATVPLGTGNNLPF+FGWGK+NP TD  AV SFL QV KA+EM ID+WHI+MRMRAP 
Sbjct: 141 PPIATVPLGTGNNLPFSFGWGKKNPTTDQEAVKSFLGQVKKAREMNIDSWHIIMRMRAPQ 200

Query: 204 EGSCDPIAPLELPHSLHAFHRVTEGD---------------------------------- 263
           EG C+PIAPLELPHSLHAFHRV+  D                                  
Sbjct: 201 EGPCEPIAPLELPHSLHAFHRVSGSDSLNMEGYHTYRGGFWNYFSMGMDAQVSYEFHSER 260

Query: 264 --------------------------------------------------------EHKV 323
                                                                   E K+
Sbjct: 261 KRNPEKFKNQLTNQSTYAKLGLKQGWFAASLTHPSSRNIAQLAKVRIMKRPGGQWEELKI 320

Query: 324 --------------------------------RDFTPPYVDDGLLEVVGFRDAWHGLVLL 383
                                           RD T P+VDDGL+EVVGFRDAWHGLVLL
Sbjct: 321 PRSIRSIVCLNLPSFSGGLNPWGTPGTRKVQDRDLTAPFVDDGLIEVVGFRDAWHGLVLL 380

Query: 384 APSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIWCKAHRIRFEFQKG 419
           AP+GHGTRLAQ                                      AHRIRFEF KG
Sbjct: 381 APNGHGTRLAQ--------------------------------------AHRIRFEFHKG 440

BLAST of CmoCh20G005910.1 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 5.6e-98
Identity = 216/499 (43.29%), Postives = 264/499 (52.91%), Query Frame = 0

Query: 19  SEFFKNFLIPDYILRPEAEAKVE-SVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLNE 78
           S+F K F IP Y+L PE E + + S    P  P+LVFINS+SGGQLG  L+ TYR+LLN+
Sbjct: 15  SDFLKKFFIPSYVLSPEDEPEAQISCTTAPENPILVFINSKSGGQLGAELILTYRTLLND 74

Query: 79  KQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVCD 138
           KQVFDL  E PD VL+R +LNLE+LK   D +A +I+ +L++IVAGGDGTAGWLLGVV D
Sbjct: 75  KQVFDLEVETPDKVLQRIYLNLERLK--DDSLASKIRDKLKIIVAGGDGTAGWLLGVVSD 134

Query: 139 LKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHILM 198
           L LS  PPIATVPLGTGNNLPFAFGWGK+NPGTD ++V SFL +V+ AKEMKIDNW ILM
Sbjct: 135 LNLSNPPPIATVPLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINAKEMKIDNWKILM 194

Query: 199 RMRAPAEGSCDPIAPLELPHSL----------------------------------HAFH 258
           RM+ P EGSCD    L+LPHSL                                  +AFH
Sbjct: 195 RMKHPKEGSCD--ITLKLPHSLPRIFPSDQENMEGYHTYRGGFWNYFSLGMDAQVSYAFH 254

Query: 259 -----------------------RVTEG-------------------------------- 318
                                    T+G                                
Sbjct: 255 SQRKLHPERFKNQLVNQSTYLKLSCTQGWFFASLFHPSSQNIAKLAKIQICDRNGQWNDL 314

Query: 319 -----------------------------DEHKVRDFTPPYVDDGLLEVVGFRDAWHGLV 378
                                         + + R  T P+VDDGL+E+VGFR+AWHGL+
Sbjct: 315 HIPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLI 374

Query: 379 LLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIWCKAHRIRFEFQ 398
           LL+P+GHGTRLAQ                                      A+R+R EF+
Sbjct: 375 LLSPNGHGTRLAQ--------------------------------------ANRVRLEFK 434

BLAST of CmoCh20G005910.1 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.5e-29
Identity = 75/186 (40.32%), Postives = 104/186 (55.91%), Query Frame = 0

Query: 27  IPDYILRPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLNEKQVFDLGEE 86
           IPD++L P            P+ P++VFIN  SGG+ G  L    + L++E+QVFDL E 
Sbjct: 72  IPDHLLLPGGAV----ADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEV 131

Query: 87  APDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVCDLK---LSYS 146
            P   +R     LEK+   GDE A E + RLR++VAGGDGT GW+LG + +L     S  
Sbjct: 132 KPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQI 191

Query: 147 PPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHILMRMRAPA 206
           PP+  +PLGTGN+L  +FGWG   P    +AV   L +       ++D+W IL+ M  P+
Sbjct: 192 PPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSM--PS 251

Query: 207 EGSCDP 210
               DP
Sbjct: 252 GEVVDP 251


HSP 2 Score: 43.5 bits (101), Expect = 6.8e-03
Identity = 18/40 (45.00%), Postives = 26/40 (65.00%), Query Frame = 0

Query: 310 KAHRIRFEFQKGKADHTFMRIDGEPWKQPLPAKESVMVEI 350
           +A  +RFE + G     F+++DGEPWKQP+  + S  VEI
Sbjct: 436 QAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEI 475

BLAST of CmoCh20G005910.1 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.2e-29
Identity = 72/203 (35.47%), Postives = 114/203 (56.16%), Query Frame = 0

Query: 40  VESVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNL 99
           +E+    P  P++VF+N +SGG+ G  +    ++L++E+QV+DL E  P+  +R     L
Sbjct: 80  LENNALTPEVPLMVFVNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCL 139

Query: 100 EKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVCDLKLSYS---PPIATVPLGTGNN 159
           E     GDE A EI++++R++VAGGDGT GW+LG + +L L      PP++ +PLGTGN+
Sbjct: 140 EAFASRGDECAKEIREKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGND 199

Query: 160 LPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELP 219
           L  +FGWG   P    +A+   L +   A   ++D+W+IL+ M  P+    DP      P
Sbjct: 200 LSRSFGWGGSFPFAWKSAIKRTLHRASVAPISRLDSWNILITM--PSGEIVDP------P 259

Query: 220 HSLHAFHRVTEGDEHKVRDFTPP 240
           +SL A          ++    PP
Sbjct: 260 YSLKATQECYIDQNLEIEGEIPP 274

BLAST of CmoCh20G005910.1 vs. ExPASy TrEMBL
Match: A0A6J1FYR8 (Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111449011 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 8.7e-203
Identity = 380/537 (70.76%), Postives = 380/537 (70.76%), Query Frame = 0

Query: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60
           MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG
Sbjct: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60

Query: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120
           GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI
Sbjct: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120

Query: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180
           VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD
Sbjct: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180

Query: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV------- 240
           QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV       
Sbjct: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKVEGCLTFR 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 GGFWNYFSMGMDAQVSYAFHSERKMHPEKFKNQLVNQSTYAKIGSTWFFAPLFHPSSMNI 300

Query: 301 ----------------------------------------------------RDFTPPYV 360
                                                               RDFTPPYV
Sbjct: 301 SQIAKVEIMKHHGDWQTLPIPPGIRSIVCLNLPSFSGGFNPWGKPNCRRQRDRDFTPPYV 360

Query: 361 DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFA 419
           DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ                             
Sbjct: 361 DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ----------------------------- 420

BLAST of CmoCh20G005910.1 vs. ExPASy TrEMBL
Match: A0A6J1FUV0 (Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111449011 PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 3.3e-202
Identity = 379/536 (70.71%), Postives = 379/536 (70.71%), Query Frame = 0

Query: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60
           MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG
Sbjct: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60

Query: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120
           GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI
Sbjct: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120

Query: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180
           VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD
Sbjct: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180

Query: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV------- 240
           QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHK        
Sbjct: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKEGCLTFRG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 GFWNYFSMGMDAQVSYAFHSERKMHPEKFKNQLVNQSTYAKIGSTWFFAPLFHPSSMNIS 300

Query: 301 ---------------------------------------------------RDFTPPYVD 360
                                                              RDFTPPYVD
Sbjct: 301 QIAKVEIMKHHGDWQTLPIPPGIRSIVCLNLPSFSGGFNPWGKPNCRRQRDRDFTPPYVD 360

Query: 361 DGLLEVVGFRDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAH 419
           DGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ                              
Sbjct: 361 DGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ------------------------------ 420

BLAST of CmoCh20G005910.1 vs. ExPASy TrEMBL
Match: A0A6J1JFP0 (Diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111484083 PE=3 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 3.5e-196
Identity = 370/537 (68.90%), Postives = 374/537 (69.65%), Query Frame = 0

Query: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60
           MC+YPKQFEAMATNF FDS+FFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG
Sbjct: 1   MCNYPKQFEAMATNFQFDSDFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60

Query: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120
           GQLGGSLLSTYRSLLNEKQVFDLGEEAPD VLRRFFLNLEKLKLNGD IAVEIQKRLRLI
Sbjct: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDAVLRRFFLNLEKLKLNGDAIAVEIQKRLRLI 120

Query: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180
           VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD
Sbjct: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180

Query: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV------- 240
           QVLKAKEMKIDNWHILMRM+APAEGSCDPIAPLELPHSLHAFHRVTEGDEH V       
Sbjct: 181 QVLKAKEMKIDNWHILMRMKAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHNVEGCLTFR 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 GGFWNYFSMGMDAQVSYAFHSERKMHPEKFKNQLVNQSTYAKIGSTWFFAPLFHPSSMNI 300

Query: 301 ----------------------------------------------------RDFTPPYV 360
                                                               RDFTPPYV
Sbjct: 301 SQIAKVEIMKHHGDWQTLPIPPGIRSIVCLNLPSFSGGFNPWGTPNCRRQRDRDFTPPYV 360

Query: 361 DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFA 419
           DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ                             
Sbjct: 361 DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ----------------------------- 420

BLAST of CmoCh20G005910.1 vs. ExPASy TrEMBL
Match: A0A6J1FW24 (Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111449011 PE=3 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 4.6e-196
Identity = 370/527 (70.21%), Postives = 370/527 (70.21%), Query Frame = 0

Query: 11  MATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLST 70
           MATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLST
Sbjct: 1   MATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLST 60

Query: 71  YRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGW 130
           YRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGW
Sbjct: 61  YRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGW 120

Query: 131 LLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKI 190
           LLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKI
Sbjct: 121 LLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKI 180

Query: 191 DNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV----------------- 250
           DNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV                 
Sbjct: 181 DNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKVEGCLTFRGGFWNYFSMG 240

Query: 251 ------------------------------------------------------------ 310
                                                                       
Sbjct: 241 MDAQVSYAFHSERKMHPEKFKNQLVNQSTYAKIGSTWFFAPLFHPSSMNISQIAKVEIMK 300

Query: 311 ------------------------------------------RDFTPPYVDDGLLEVVGF 370
                                                     RDFTPPYVDDGLLEVVGF
Sbjct: 301 HHGDWQTLPIPPGIRSIVCLNLPSFSGGFNPWGKPNCRRQRDRDFTPPYVDDGLLEVVGF 360

Query: 371 RDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIWCKA 419
           RDAWHGLVLLAPSGHGTRLAQ                                      A
Sbjct: 361 RDAWHGLVLLAPSGHGTRLAQ--------------------------------------A 420

BLAST of CmoCh20G005910.1 vs. ExPASy TrEMBL
Match: A0A6J1JDP0 (Diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111484083 PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 1.0e-195
Identity = 369/536 (68.84%), Postives = 374/536 (69.78%), Query Frame = 0

Query: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60
           MC+YPKQFEAMATNF FDS+FFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG
Sbjct: 1   MCNYPKQFEAMATNFQFDSDFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60

Query: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120
           GQLGGSLLSTYRSLLNEKQVFDLGEEAPD VLRRFFLNLEKLKLNGD IAVEIQKRLRLI
Sbjct: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDAVLRRFFLNLEKLKLNGDAIAVEIQKRLRLI 120

Query: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180
           VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD
Sbjct: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180

Query: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEH--------- 240
           QVLKAKEMKIDNWHILMRM+APAEGSCDPIAPLELPHSLHAFHRVTEGDEH         
Sbjct: 181 QVLKAKEMKIDNWHILMRMKAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHNEGCLTFRG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 GFWNYFSMGMDAQVSYAFHSERKMHPEKFKNQLVNQSTYAKIGSTWFFAPLFHPSSMNIS 300

Query: 301 -------------------------------------------------KVRDFTPPYVD 360
                                                            + RDFTPPYVD
Sbjct: 301 QIAKVEIMKHHGDWQTLPIPPGIRSIVCLNLPSFSGGFNPWGTPNCRRQRDRDFTPPYVD 360

Query: 361 DGLLEVVGFRDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAH 419
           DGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ                              
Sbjct: 361 DGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ------------------------------ 420

BLAST of CmoCh20G005910.1 vs. NCBI nr
Match: XP_022944600.1 (diacylglycerol kinase 5-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 716.1 bits (1847), Expect = 1.8e-202
Identity = 380/537 (70.76%), Postives = 380/537 (70.76%), Query Frame = 0

Query: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60
           MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG
Sbjct: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60

Query: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120
           GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI
Sbjct: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120

Query: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180
           VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD
Sbjct: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180

Query: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV------- 240
           QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV       
Sbjct: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKVEGCLTFR 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 GGFWNYFSMGMDAQVSYAFHSERKMHPEKFKNQLVNQSTYAKIGSTWFFAPLFHPSSMNI 300

Query: 301 ----------------------------------------------------RDFTPPYV 360
                                                               RDFTPPYV
Sbjct: 301 SQIAKVEIMKHHGDWQTLPIPPGIRSIVCLNLPSFSGGFNPWGKPNCRRQRDRDFTPPYV 360

Query: 361 DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFA 419
           DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ                             
Sbjct: 361 DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ----------------------------- 420

BLAST of CmoCh20G005910.1 vs. NCBI nr
Match: XP_022944601.1 (diacylglycerol kinase 5-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 714.1 bits (1842), Expect = 6.8e-202
Identity = 379/536 (70.71%), Postives = 379/536 (70.71%), Query Frame = 0

Query: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60
           MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG
Sbjct: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60

Query: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120
           GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI
Sbjct: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120

Query: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180
           VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD
Sbjct: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180

Query: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV------- 240
           QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHK        
Sbjct: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKEGCLTFRG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 GFWNYFSMGMDAQVSYAFHSERKMHPEKFKNQLVNQSTYAKIGSTWFFAPLFHPSSMNIS 300

Query: 301 ---------------------------------------------------RDFTPPYVD 360
                                                              RDFTPPYVD
Sbjct: 301 QIAKVEIMKHHGDWQTLPIPPGIRSIVCLNLPSFSGGFNPWGKPNCRRQRDRDFTPPYVD 360

Query: 361 DGLLEVVGFRDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAH 419
           DGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ                              
Sbjct: 361 DGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ------------------------------ 420

BLAST of CmoCh20G005910.1 vs. NCBI nr
Match: XP_023512855.1 (diacylglycerol kinase 5-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 699.5 bits (1804), Expect = 1.7e-197
Identity = 372/537 (69.27%), Postives = 374/537 (69.65%), Query Frame = 0

Query: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60
           MCSYPKQFEAMATNF FDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG
Sbjct: 1   MCSYPKQFEAMATNFQFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60

Query: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120
           GQLGGSLLSTYRSLLNEKQVFDLG+EAPD VLRRFFLNLEKLKLNGD IAVEIQKRLRLI
Sbjct: 61  GQLGGSLLSTYRSLLNEKQVFDLGDEAPDAVLRRFFLNLEKLKLNGDAIAVEIQKRLRLI 120

Query: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180
           VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD
Sbjct: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180

Query: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV------- 240
           QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDE  V       
Sbjct: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEDNVEGCLTFR 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 GGFWNYFSMGMDAQVSYAFHSERKMHPEKFKNQLVNQSTYAKIGSTWFFAPLFHPSSMNI 300

Query: 301 ----------------------------------------------------RDFTPPYV 360
                                                               RDFTPPYV
Sbjct: 301 SQIAKVEIMKHHGDWQTLPIPPGIRSIVCLNLPSFSGGFNPWGKPNCRRRRDRDFTPPYV 360

Query: 361 DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFA 419
           DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ                             
Sbjct: 361 DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ----------------------------- 420

BLAST of CmoCh20G005910.1 vs. NCBI nr
Match: KAG6570854.1 (Diacylglycerol kinase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 695.3 bits (1793), Expect = 3.3e-196
Identity = 370/530 (69.81%), Postives = 372/530 (70.19%), Query Frame = 0

Query: 7   QFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGS 66
           +FEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGS
Sbjct: 381 RFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGS 440

Query: 67  LLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDG 126
           LLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDG
Sbjct: 441 LLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDG 500

Query: 127 TAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAK 186
           TAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAK
Sbjct: 501 TAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAK 560

Query: 187 EMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV------------- 246
           EMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHK              
Sbjct: 561 EMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKEGCLTFRGGFWNYF 620

Query: 247 ------------------------------------------------------------ 306
                                                                       
Sbjct: 621 SMGMDAQVSYAFHSERKMHPEKFKNQLVNQSTYAKIGSTWFFAPLFHPSSMNISQIAKVE 680

Query: 307 ---------------------------------------------RDFTPPYVDDGLLEV 366
                                                        RDFTPPYVDDGLLEV
Sbjct: 681 IMKHHGDWQTLPIPPGIRSIVCLNLPSFSGGFNPWGKPNCRRQRDRDFTPPYVDDGLLEV 740

Query: 367 VGFRDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIW 419
           VGFRDAWHGLVLLAPSGHGTRLAQ                                    
Sbjct: 741 VGFRDAWHGLVLLAPSGHGTRLAQ------------------------------------ 800

BLAST of CmoCh20G005910.1 vs. NCBI nr
Match: XP_022986294.1 (diacylglycerol kinase 5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 694.1 bits (1790), Expect = 7.3e-196
Identity = 370/537 (68.90%), Postives = 374/537 (69.65%), Query Frame = 0

Query: 1   MCSYPKQFEAMATNFPFDSEFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60
           MC+YPKQFEAMATNF FDS+FFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG
Sbjct: 1   MCNYPKQFEAMATNFQFDSDFFKNFLIPDYILRPEAEAKVESVPYVPNCPVLVFINSRSG 60

Query: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLI 120
           GQLGGSLLSTYRSLLNEKQVFDLGEEAPD VLRRFFLNLEKLKLNGD IAVEIQKRLRLI
Sbjct: 61  GQLGGSLLSTYRSLLNEKQVFDLGEEAPDAVLRRFFLNLEKLKLNGDAIAVEIQKRLRLI 120

Query: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180
           VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD
Sbjct: 121 VAGGDGTAGWLLGVVCDLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLD 180

Query: 181 QVLKAKEMKIDNWHILMRMRAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHKV------- 240
           QVLKAKEMKIDNWHILMRM+APAEGSCDPIAPLELPHSLHAFHRVTEGDEH V       
Sbjct: 181 QVLKAKEMKIDNWHILMRMKAPAEGSCDPIAPLELPHSLHAFHRVTEGDEHNVEGCLTFR 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 GGFWNYFSMGMDAQVSYAFHSERKMHPEKFKNQLVNQSTYAKIGSTWFFAPLFHPSSMNI 300

Query: 301 ----------------------------------------------------RDFTPPYV 360
                                                               RDFTPPYV
Sbjct: 301 SQIAKVEIMKHHGDWQTLPIPPGIRSIVCLNLPSFSGGFNPWGTPNCRRQRDRDFTPPYV 360

Query: 361 DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFA 419
           DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ                             
Sbjct: 361 DDGLLEVVGFRDAWHGLVLLAPSGHGTRLAQ----------------------------- 420

BLAST of CmoCh20G005910.1 vs. TAIR 10
Match: AT2G20900.3 (diacylglycerol kinase 5 )

HSP 1 Score: 472.2 bits (1214), Expect = 4.2e-133
Identity = 272/525 (51.81%), Postives = 303/525 (57.71%), Query Frame = 0

Query: 19  SEFFKNFLIPDYIL--RPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLN 78
           S+F K F IP Y+L    E E + ES P  P  PVLVFINS+SGGQLGG L+ TYRSLLN
Sbjct: 8   SDFLKEFYIPTYVLSAETEEEEEEESRP-TPASPVLVFINSKSGGQLGGELILTYRSLLN 67

Query: 79  EKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVC 138
             QVFDL +E PD VLRR +LNLE+LK   D+ A +I+++L++IVAGGDGTAGWLLGVVC
Sbjct: 68  HNQVFDLDQETPDKVLRRIYLNLERLK--DDDFARQIREKLKIIVAGGDGTAGWLLGVVC 127

Query: 139 DLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHIL 198
           DLKLS+ PPIATVPLGTGNNLPFAFGWGK+NPGTD  AV SFL+QVLKAK MKIDNWHIL
Sbjct: 128 DLKLSHPPPIATVPLGTGNNLPFAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHIL 187

Query: 199 MRMRAPAE-GSCDPIAPLELPHSLHAFHRVTEGDE------------------------- 258
           MRM+ P E GSCDP+APLELPHSLHAFHRV+  DE                         
Sbjct: 188 MRMKTPKEGGSCDPVAPLELPHSLHAFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQI 247

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 248 SYAFHSERKLHPEKFKNQLVNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNG 307

Query: 319 --------HKVRD----------------------------FTPPYVDDGLLEVVGFRDA 378
                   H +R                              TPP+VDDGL+EVVGFR+A
Sbjct: 308 QWQDLHIPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNA 367

Query: 379 WHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIWCKAHRI 419
           WHGLVLLAP+GHGTRLAQ                                      A+RI
Sbjct: 368 WHGLVLLAPNGHGTRLAQ--------------------------------------ANRI 427

BLAST of CmoCh20G005910.1 vs. TAIR 10
Match: AT2G20900.2 (diacylglycerol kinase 5 )

HSP 1 Score: 472.2 bits (1214), Expect = 4.2e-133
Identity = 272/525 (51.81%), Postives = 303/525 (57.71%), Query Frame = 0

Query: 19  SEFFKNFLIPDYIL--RPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLN 78
           S+F K F IP Y+L    E E + ES P  P  PVLVFINS+SGGQLGG L+ TYRSLLN
Sbjct: 8   SDFLKEFYIPTYVLSAETEEEEEEESRP-TPASPVLVFINSKSGGQLGGELILTYRSLLN 67

Query: 79  EKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVC 138
             QVFDL +E PD VLRR +LNLE+LK   D+ A +I+++L++IVAGGDGTAGWLLGVVC
Sbjct: 68  HNQVFDLDQETPDKVLRRIYLNLERLK--DDDFARQIREKLKIIVAGGDGTAGWLLGVVC 127

Query: 139 DLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHIL 198
           DLKLS+ PPIATVPLGTGNNLPFAFGWGK+NPGTD  AV SFL+QVLKAK MKIDNWHIL
Sbjct: 128 DLKLSHPPPIATVPLGTGNNLPFAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHIL 187

Query: 199 MRMRAPAE-GSCDPIAPLELPHSLHAFHRVTEGDE------------------------- 258
           MRM+ P E GSCDP+APLELPHSLHAFHRV+  DE                         
Sbjct: 188 MRMKTPKEGGSCDPVAPLELPHSLHAFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQI 247

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 248 SYAFHSERKLHPEKFKNQLVNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNG 307

Query: 319 --------HKVRD----------------------------FTPPYVDDGLLEVVGFRDA 378
                   H +R                              TPP+VDDGL+EVVGFR+A
Sbjct: 308 QWQDLHIPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNA 367

Query: 379 WHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIWCKAHRI 419
           WHGLVLLAP+GHGTRLAQ                                      A+RI
Sbjct: 368 WHGLVLLAPNGHGTRLAQ--------------------------------------ANRI 427

BLAST of CmoCh20G005910.1 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 459.5 bits (1181), Expect = 2.8e-129
Identity = 265/518 (51.16%), Postives = 296/518 (57.14%), Query Frame = 0

Query: 19  SEFFKNFLIPDYIL--RPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLN 78
           S+F K F IP Y+L    E E + ES P  P  PVLVFINS+SGGQLGG L+ TYRSLLN
Sbjct: 8   SDFLKEFYIPTYVLSAETEEEEEEESRP-TPASPVLVFINSKSGGQLGGELILTYRSLLN 67

Query: 79  EKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVC 138
             QVFDL +E PD VLRR +LNLE+LK   D+ A +I+++L++IVAGGDGTAGWLLGVVC
Sbjct: 68  HNQVFDLDQETPDKVLRRIYLNLERLK--DDDFARQIREKLKIIVAGGDGTAGWLLGVVC 127

Query: 139 DLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHIL 198
           DLKLS+ PPIATVPLGTGNNLPFAFGWGK+NPGTD  AV SFL+QVLKAK MKIDNWHIL
Sbjct: 128 DLKLSHPPPIATVPLGTGNNLPFAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHIL 187

Query: 199 MRMRAPAE-GSCDPIAPLELPHSLHAFHRVTEGDE------------------------- 258
           MRM+ P E GSCDP+APLELPHSLHAFHRV+  DE                         
Sbjct: 188 MRMKTPKEGGSCDPVAPLELPHSLHAFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQI 247

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 248 SYAFHSERKLHPEKFKNQLVNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNG 307

Query: 319 --------HKVRD----------------------------FTPPYVDDGLLEVVGFRDA 378
                   H +R                              TPP+VDDGL+EVVGFR+A
Sbjct: 308 QWQDLHIPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNA 367

Query: 379 WHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIWCKAHRI 412
           WHGLVLLAP+GHGTRLAQ                                      A+RI
Sbjct: 368 WHGLVLLAPNGHGTRLAQ--------------------------------------ANRI 427

BLAST of CmoCh20G005910.1 vs. TAIR 10
Match: AT2G20900.4 (diacylglycerol kinase 5 )

HSP 1 Score: 459.5 bits (1181), Expect = 2.8e-129
Identity = 265/518 (51.16%), Postives = 296/518 (57.14%), Query Frame = 0

Query: 19  SEFFKNFLIPDYIL--RPEAEAKVESVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLN 78
           S+F K F IP Y+L    E E + ES P  P  PVLVFINS+SGGQLGG L+ TYRSLLN
Sbjct: 8   SDFLKEFYIPTYVLSAETEEEEEEESRP-TPASPVLVFINSKSGGQLGGELILTYRSLLN 67

Query: 79  EKQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVC 138
             QVFDL +E PD VLRR +LNLE+LK   D+ A +I+++L++IVAGGDGTAGWLLGVVC
Sbjct: 68  HNQVFDLDQETPDKVLRRIYLNLERLK--DDDFARQIREKLKIIVAGGDGTAGWLLGVVC 127

Query: 139 DLKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHIL 198
           DLKLS+ PPIATVPLGTGNNLPFAFGWGK+NPGTD  AV SFL+QVLKAK MKIDNWHIL
Sbjct: 128 DLKLSHPPPIATVPLGTGNNLPFAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHIL 187

Query: 199 MRMRAPAE-GSCDPIAPLELPHSLHAFHRVTEGDE------------------------- 258
           MRM+ P E GSCDP+APLELPHSLHAFHRV+  DE                         
Sbjct: 188 MRMKTPKEGGSCDPVAPLELPHSLHAFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQI 247

Query: 259 ------------------------------------------------------------ 318
                                                                       
Sbjct: 248 SYAFHSERKLHPEKFKNQLVNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKVKIATRNG 307

Query: 319 --------HKVRD----------------------------FTPPYVDDGLLEVVGFRDA 378
                   H +R                              TPP+VDDGL+EVVGFR+A
Sbjct: 308 QWQDLHIPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNA 367

Query: 379 WHGLVLLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIWCKAHRI 412
           WHGLVLLAP+GHGTRLAQ                                      A+RI
Sbjct: 368 WHGLVLLAPNGHGTRLAQ--------------------------------------ANRI 427

BLAST of CmoCh20G005910.1 vs. TAIR 10
Match: AT4G28130.1 (diacylglycerol kinase 6 )

HSP 1 Score: 359.4 bits (921), Expect = 4.0e-99
Identity = 216/499 (43.29%), Postives = 264/499 (52.91%), Query Frame = 0

Query: 19  SEFFKNFLIPDYILRPEAEAKVE-SVPYVPNCPVLVFINSRSGGQLGGSLLSTYRSLLNE 78
           S+F K F IP Y+L PE E + + S    P  P+LVFINS+SGGQLG  L+ TYR+LLN+
Sbjct: 15  SDFLKKFFIPSYVLSPEDEPEAQISCTTAPENPILVFINSKSGGQLGAELILTYRTLLND 74

Query: 79  KQVFDLGEEAPDVVLRRFFLNLEKLKLNGDEIAVEIQKRLRLIVAGGDGTAGWLLGVVCD 138
           KQVFDL  E PD VL+R +LNLE+LK   D +A +I+ +L++IVAGGDGTAGWLLGVV D
Sbjct: 75  KQVFDLEVETPDKVLQRIYLNLERLK--DDSLASKIRDKLKIIVAGGDGTAGWLLGVVSD 134

Query: 139 LKLSYSPPIATVPLGTGNNLPFAFGWGKRNPGTDLNAVISFLDQVLKAKEMKIDNWHILM 198
           L LS  PPIATVPLGTGNNLPFAFGWGK+NPGTD ++V SFL +V+ AKEMKIDNW ILM
Sbjct: 135 LNLSNPPPIATVPLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINAKEMKIDNWKILM 194

Query: 199 RMRAPAEGSCDPIAPLELPHSL----------------------------------HAFH 258
           RM+ P EGSCD    L+LPHSL                                  +AFH
Sbjct: 195 RMKHPKEGSCD--ITLKLPHSLPRIFPSDQENMEGYHTYRGGFWNYFSLGMDAQVSYAFH 254

Query: 259 -----------------------RVTEG-------------------------------- 318
                                    T+G                                
Sbjct: 255 SQRKLHPERFKNQLVNQSTYLKLSCTQGWFFASLFHPSSQNIAKLAKIQICDRNGQWNDL 314

Query: 319 -----------------------------DEHKVRDFTPPYVDDGLLEVVGFRDAWHGLV 378
                                         + + R  T P+VDDGL+E+VGFR+AWHGL+
Sbjct: 315 HIPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLI 374

Query: 379 LLAPSGHGTRLAQLWIITFSIWDIGYVMRYCSEFVCVSVVFAHTSPLIWCKAHRIRFEFQ 398
           LL+P+GHGTRLAQ                                      A+R+R EF+
Sbjct: 375 LLSPNGHGTRLAQ--------------------------------------ANRVRLEFK 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C5E54.0e-12851.16Diacylglycerol kinase 5 OS=Arabidopsis thaliana OX=3702 GN=DGK5 PE=2 SV=1[more]
Q7XQT22.8e-12148.36Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV... [more]
F4JKI35.6e-9843.29Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1[more]
Q8VZG11.5e-2940.32Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1[more]
Q1PDI23.2e-2935.47Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FYR88.7e-20370.76Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111449011 PE=3 SV=1[more]
A0A6J1FUV03.3e-20270.71Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111449011 PE=3 SV=1[more]
A0A6J1JFP03.5e-19668.90Diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111484083 PE=3 SV=1[more]
A0A6J1FW244.6e-19670.21Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111449011 PE=3 SV=1[more]
A0A6J1JDP01.0e-19568.84Diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111484083 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022944600.11.8e-20270.76diacylglycerol kinase 5-like isoform X1 [Cucurbita moschata][more]
XP_022944601.16.8e-20270.71diacylglycerol kinase 5-like isoform X2 [Cucurbita moschata][more]
XP_023512855.11.7e-19769.27diacylglycerol kinase 5-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6570854.13.3e-19669.81Diacylglycerol kinase 5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022986294.17.3e-19668.90diacylglycerol kinase 5-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G20900.34.2e-13351.81diacylglycerol kinase 5 [more]
AT2G20900.24.2e-13351.81diacylglycerol kinase 5 [more]
AT2G20900.12.8e-12951.16diacylglycerol kinase 5 [more]
AT2G20900.42.8e-12951.16diacylglycerol kinase 5 [more]
AT4G28130.14.0e-9943.29diacylglycerol kinase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001206Diacylglycerol kinase, catalytic domainSMARTSM00046dagk_c4a_7coord: 50..193
e-value: 1.1E-19
score: 81.4
IPR001206Diacylglycerol kinase, catalytic domainPFAMPF00781DAGK_catcoord: 50..176
e-value: 3.8E-24
score: 84.7
IPR001206Diacylglycerol kinase, catalytic domainPROSITEPS50146DAGKcoord: 46..199
score: 22.723284
IPR017438Inorganic polyphosphate/ATP-NAD kinase, N-terminalGENE3D3.40.50.10330coord: 49..195
e-value: 1.0E-13
score: 52.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 367..394
NoneNo IPR availablePANTHERPTHR11255:SF99DIACYLGLYCEROL KINASEcoord: 232..279
coord: 19..231
NoneNo IPR availablePANTHERPTHR11255:SF99DIACYLGLYCEROL KINASEcoord: 309..418
IPR037607Diacylglycerol kinasePANTHERPTHR11255DIACYLGLYCEROL KINASEcoord: 232..279
coord: 309..418
coord: 19..231
IPR016064NAD kinase/diacylglycerol kinase-like domain superfamilySUPERFAMILY111331NAD kinase/diacylglycerol kinase-likecoord: 48..342

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh20G005910CmoCh20G005910gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh20G005910.1:exon:2043CmoCh20G005910.1:exon:2043exon
CmoCh20G005910.1:exon:2042CmoCh20G005910.1:exon:2042exon
CmoCh20G005910.1:exon:2041CmoCh20G005910.1:exon:2041exon
CmoCh20G005910.1:exon:2040CmoCh20G005910.1:exon:2040exon
CmoCh20G005910.1:exon:2039CmoCh20G005910.1:exon:2039exon
CmoCh20G005910.1:exon:2038CmoCh20G005910.1:exon:2038exon
CmoCh20G005910.1:exon:2037CmoCh20G005910.1:exon:2037exon
CmoCh20G005910.1:exon:2036CmoCh20G005910.1:exon:2036exon
CmoCh20G005910.1:exon:2035CmoCh20G005910.1:exon:2035exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh20G005910.1:cdsCmoCh20G005910.1:cds_9CDS
CmoCh20G005910.1:cdsCmoCh20G005910.1:cds_8CDS
CmoCh20G005910.1:cdsCmoCh20G005910.1:cds_7CDS
CmoCh20G005910.1:cdsCmoCh20G005910.1:cds_6CDS
CmoCh20G005910.1:cdsCmoCh20G005910.1:cds_5CDS
CmoCh20G005910.1:cdsCmoCh20G005910.1:cds_4CDS
CmoCh20G005910.1:cdsCmoCh20G005910.1:cds_3CDS
CmoCh20G005910.1:cdsCmoCh20G005910.1:cds_2CDS
CmoCh20G005910.1:cdsCmoCh20G005910.1:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh20G005910.1CmoCh20G005910.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007165 signal transduction
molecular_function GO:0004143 diacylglycerol kinase activity
molecular_function GO:0016301 kinase activity
molecular_function GO:0003951 NAD+ kinase activity
molecular_function GO:0000166 nucleotide binding