CmoCh16G010990.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G010990.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative
Location	Cmo_Chr16: 7753107 .. 7753673 (+)
Sequence length	567
RNA-Seq Expression	CmoCh16G010990.1
Synteny	CmoCh16G010990.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATCCTCAGTTCTTCCAATCACAGCCATTGTCTTTGTGATCTTAATCAACCAATCATATACTGTTGCAACTCAACCAATCAACGACACCCAATTCATCAAAACCACTTGTCAAACCACACCCTACCCTGACCTCTGCCTTTCCTCCCTCTCCCCTGACGCCTCCGCCATTCGCTGCAGCCATCGTCTGATGACTATTGCCGCCCTCGCCGTTGCTCTCACGCACACGCATGATGCCTCATCGACCGTCACGAGCCTGGCCAAGTCGAATGCCATAACAACGCGAGAGGGTTATGCTTTGAGTGATTGCATTGAGGAGTTTGGAGACTCAGTAGAAGAGTTAAAGATGGCTGTGGAAGCGTTGAAGGGTAATGACAAAACCCGACTTGACACTGAAGATATTCAAACGTGGATAAGCGCTGCTCTAACTGACGACAATACGTGCATGGACGGGTTTGCAGGAGACGCCATGAACGGTAGCGTGAAGGAGTCGATGAAGGCAATGGTGGTGGATGTTGCTCAATTGACAAGCATTGCTCTCTCGCTTGTTAGGCAGTTGAAGTGA

mRNA sequence

ATGGAATCCTCAGTTCTTCCAATCACAGCCATTGTCTTTGTGATCTTAATCAACCAATCATATACTGTTGCAACTCAACCAATCAACGACACCCAATTCATCAAAACCACTTGTCAAACCACACCCTACCCTGACCTCTGCCTTTCCTCCCTCTCCCCTGACGCCTCCGCCATTCGCTGCAGCCATCGTCTGATGACTATTGCCGCCCTCGCCGTTGCTCTCACGCACACGCATGATGCCTCATCGACCGTCACGAGCCTGGCCAAGTCGAATGCCATAACAACGCGAGAGGGTTATGCTTTGAGTGATTGCATTGAGGAGTTTGGAGACTCAGTAGAAGAGTTAAAGATGGCTGTGGAAGCGTTGAAGGGTAATGACAAAACCCGACTTGACACTGAAGATATTCAAACGTGGATAAGCGCTGCTCTAACTGACGACAATACGTGCATGGACGGGTTTGCAGGAGACGCCATGAACGGTAGCGTGAAGGAGTCGATGAAGGCAATGGTGGTGGATGTTGCTCAATTGACAAGCATTGCTCTCTCGCTTGTTAGGCAGTTGAAGTGA

Coding sequence (CDS)

ATGGAATCCTCAGTTCTTCCAATCACAGCCATTGTCTTTGTGATCTTAATCAACCAATCATATACTGTTGCAACTCAACCAATCAACGACACCCAATTCATCAAAACCACTTGTCAAACCACACCCTACCCTGACCTCTGCCTTTCCTCCCTCTCCCCTGACGCCTCCGCCATTCGCTGCAGCCATCGTCTGATGACTATTGCCGCCCTCGCCGTTGCTCTCACGCACACGCATGATGCCTCATCGACCGTCACGAGCCTGGCCAAGTCGAATGCCATAACAACGCGAGAGGGTTATGCTTTGAGTGATTGCATTGAGGAGTTTGGAGACTCAGTAGAAGAGTTAAAGATGGCTGTGGAAGCGTTGAAGGGTAATGACAAAACCCGACTTGACACTGAAGATATTCAAACGTGGATAAGCGCTGCTCTAACTGACGACAATACGTGCATGGACGGGTTTGCAGGAGACGCCATGAACGGTAGCGTGAAGGAGTCGATGAAGGCAATGGTGGTGGATGTTGCTCAATTGACAAGCATTGCTCTCTCGCTTGTTAGGCAGTTGAAGTGA

Protein sequence

MESSVLPITAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLMTIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVEALKGNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLVRQLK

Homology

[Close]

BLAST of CmoCh16G010990.1 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 74/182 (40.66%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 9   TAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLMTIA 68
           + +VF  L+      +  P N  QFIKT+C  T YP +C  SLS  A  I+ + + +   
Sbjct: 6   STLVFSALLVILAAASAAPAN--QFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELAST 65

Query: 69  ALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVEAL------ 128
           AL V+LT T  A + +  L K   +  R+  A+ DC+EE  DS++ +  + + +      
Sbjct: 66  ALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHA 125

Query: 129 KGNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALS 185
           KGND T     +++TW+SAALTD+ TCMDGFAG  M+G +KES++A VV VA++TS AL+
Sbjct: 126 KGNDFT-FRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALA 184

[Close]

BLAST of CmoCh16G010990.1 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 7.0e-24
Identity = 69/188 (36.70%), Postives = 109/188 (57.98%), Query Frame = 0


Query: 8   ITAIVFVILINQSYTVATQPI-----NDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSH 67
           ++ I+FV+ ++ +  V  + +      DT+FIK +C+TT YPD C  SLS  AS I+   
Sbjct: 7   LSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQP 66

Query: 68  RLMTIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVEAL 127
           R +   ALAV++     A + V+ +     IT R+  A++DC+EE GD+V+ L  +++ L
Sbjct: 67  RKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKEL 126

Query: 128 K------GNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQL 185
           K        +       +++TW SAALTD+  CMDGF G AM G +K  ++  +V VA+ 
Sbjct: 127 KHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEE 186

[Close]

BLAST of CmoCh16G010990.1 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 3.8e-22
Identity = 63/157 (40.13%), Postives = 100/157 (63.69%), Query Frame = 0


Query: 33  FIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLMTIAALAVALTHTHDASSTVTSLAKSNA 92
           +IKT+C  T Y  +C +SLSP AS IR + + + + AL + L+    AS  V +++    
Sbjct: 147 YIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGG 206

Query: 93  ITTREGYALSDCIEEFGDSVEELKMAVEAL-----KGNDKTRLDTEDIQTWISAALTDDN 152
           +T  E  A++DC+EE GDSV  L+ ++  L     K + K  +   D++TW+SAALT+D+
Sbjct: 207 LTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDD 266

Query: 153 TCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLV 185
           TCMDGF+   +  +VK+ ++  VV+VA+LTS AL+L+
Sbjct: 267 TCMDGFS--LVKTAVKDLVRRHVVEVARLTSNALALI 301

[Close]

BLAST of CmoCh16G010990.1 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.2e-21
Identity = 61/158 (38.61%), Postives = 95/158 (60.13%), Query Frame = 0


Query: 33  FIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLMTIAALAVALTHTHDASSTVTSLAKSNA 92
           FI+ +C+ T YP +C++SL+  A++I+ S R +   AL V +T        V  L +  +
Sbjct: 38  FIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTS 97

Query: 93  ITTREGYALSDCIEEFGDSVEELKMAVE------ALKGNDKTRLDTEDIQTWISAALTDD 152
           +  RE  A+ DCIEE  D+V+ L M++       + KG D+      + QTW SAALT+ 
Sbjct: 98  LKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNA 157

Query: 153 NTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLV 185
           NTC DGFAG  M+G VK S++A ++++ + TS AL+L+
Sbjct: 158 NTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALI 195

[Close]

BLAST of CmoCh16G010990.1 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.7e-20
Identity = 71/184 (38.59%), Postives = 102/184 (55.43%), Query Frame = 0


Query: 11  IVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSH-RLMTIAA 70
           I+  I I QS +         QFI ++CQTT YP LC+ +LS  A+ IR ++ + +   A
Sbjct: 11  ILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTA 70

Query: 71  LAVALTHTHDASSTVTSLAKSN-AITTREGYALSDCIEEFGDSVEELKMAVEALK----- 130
           L ++L      +  V  L K       RE  A+ DCIE  G+SV+ L  +V+ L      
Sbjct: 71  LIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHA 130

Query: 131 -GNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALS 187
             ++       ++QTW+SAALTD+ TC+DGF+  AM G VK  ++  VV VAQ+TS AL+
Sbjct: 131 VASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALA 190

[Close]

BLAST of CmoCh16G010990.1 vs. ExPASy TrEMBL
Match: A0A6J1E6H9 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 3.3e-93
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MESSVLPITAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRC 60
           MESSVLPITAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRC
Sbjct: 1   MESSVLPITAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRC 60

Query: 61  SHRLMTIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVE 120
           SHRLMTIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVE
Sbjct: 61  SHRLMTIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVE 120

Query: 121 ALKGNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIA 180
           ALKGNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIA
Sbjct: 121 ALKGNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIA 180

Query: 181 LSLVRQLK 189
           LSLVRQLK
Sbjct: 181 LSLVRQLK 188

[Close]

BLAST of CmoCh16G010990.1 vs. ExPASy TrEMBL
Match: A0A1S3BLP3 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 4.6e-63
Identity = 138/192 (71.88%), Postives = 158/192 (82.29%), Query Frame = 0


Query: 1   MESSVLPI---TAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASA 60
           M+SS  P+     ++ +ILINQS    +QP NDTQFIKTTCQ+TPYPDLCLSSLS  A+A
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  IRCSHRLMTIAALAVALTHTHDASSTVTSLAK-SNAITTREGYALSDCIEEFGDSVEELK 120
           I  S+RLMTIAAL VALTHT   SS V SLAK SNA+T REGY + DCIEEFGDSVEELK
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 MAVEALKGNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQL 180
           MA+E LK N+KTR +TEDI+TW+SAALTDD+TCMDG  GDAMNG++KES+KAMVVDVAQL
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVRQLK 189
           TSIALSLV  LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of CmoCh16G010990.1 vs. ExPASy TrEMBL
Match: A0A0A0L1P3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.8e-60
Identity = 132/185 (71.35%), Postives = 154/185 (83.24%), Query Frame = 0


Query: 6   LPITAIVFVILINQSYTVA-TQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRL 65
           LP+ AI+ ++LINQS  +A +QPINDTQFIKTTCQ+TPYPDLCLSSLS  A+ I  S  L
Sbjct: 8   LPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHL 67

Query: 66  MTIAALAVALTHTHDASSTVTSLAK-SNAITTREGYALSDCIEEFGDSVEELKMAVEALK 125
           MT+AAL VALTHT   SS + SLAK SNA+T R+ Y + DCIEEFGDSVEELKMAVE LK
Sbjct: 68  MTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELK 127

Query: 126 GNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSL 185
            N+K+R +TEDI+TW+SAALTDD+TCMDG  GDAMNG+VKES+K MVV+VAQLTSIALSL
Sbjct: 128 DNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSL 187

Query: 186 VRQLK 189
           V  LK
Sbjct: 188 VSLLK 192

[Close]

BLAST of CmoCh16G010990.1 vs. ExPASy TrEMBL
Match: A0A6J1DFW8 (pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 PE=4 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 3.5e-50
Identity = 119/183 (65.03%), Postives = 141/183 (77.05%), Query Frame = 0


Query: 6   LPITAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLM 65
           LPITAI+ +ILIN    ++   I+DTQFIK TCQTTPYPDLC SSLS DA ++  S R M
Sbjct: 8   LPITAILLIILIN----LSNATIDDTQFIKATCQTTPYPDLCFSSLSADAVSVHGSLRSM 67

Query: 66  TIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVEALKGN 125
           TIAAL VALTHT  ASS V SLAKSNA+  +E +AL DCIEE GDSVEEL+ +V  LK  
Sbjct: 68  TIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQSSVAELKDG 127

Query: 126 DKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLVR 185
           D +R +T+DI+TW+SAALTDD+TCM+G  GD   G VKES++ +VVDVAQLTSIALSLV 
Sbjct: 128 DGSRPETDDIRTWVSAALTDDDTCMEGLVGD--GGGVKESVRGLVVDVAQLTSIALSLVS 184

Query: 186 QLK 189
            LK
Sbjct: 188 HLK 184

[Close]

BLAST of CmoCh16G010990.1 vs. ExPASy TrEMBL
Match: A0A5D3D7I0 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001400 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 7.7e-42
Identity = 97/125 (77.60%), Postives = 108/125 (86.40%), Query Frame = 0


Query: 65  MTIAALAVALTHTHDASSTVTSLAK-SNAITTREGYALSDCIEEFGDSVEELKMAVEALK 124
           MTIAAL VALTHT   SS V SLAK SNA+T REGY + DCIEEFGDSVEELKMA+E LK
Sbjct: 1   MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK 60

Query: 125 GNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSL 184
            N+KTR +TEDI+TW+SAALTDD+TCMDG  GDAMNG++KES+KAMVVDVAQLTSIALSL
Sbjct: 61  DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSL 120

Query: 185 VRQLK 189
           V  LK
Sbjct: 121 VSLLK 125

[Close]

BLAST of CmoCh16G010990.1 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 115.2 bits (287), Expect = 5.9e-26
Identity = 70/176 (39.77%), Postives = 106/176 (60.23%), Query Frame = 0


Query: 11  IVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLMTIAAL 70
           I  + L++ + T  T    +T+F+K++C  T YP LC SSLS  AS I+ S +LM  AAL
Sbjct: 21  IAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAAL 80

Query: 71  AVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVEAL--KGNDKT 130
            + L      S+ +  L+ S  +  +E  A+ DC+EE GD++EEL+ ++  +        
Sbjct: 81  NITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSNY 140

Query: 131 RLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLV 185
            +   DIQTW+SAALTD NTC DGF G+ M+G VK  ++  ++ +A LTS AL+L+
Sbjct: 141 EVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALI 195

[Close]

BLAST of CmoCh16G010990.1 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 112.1 bits (279), Expect = 5.0e-25
Identity = 69/188 (36.70%), Postives = 109/188 (57.98%), Query Frame = 0


Query: 8   ITAIVFVILINQSYTVATQPI-----NDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSH 67
           ++ I+FV+ ++ +  V  + +      DT+FIK +C+TT YPD C  SLS  AS I+   
Sbjct: 7   LSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQP 66

Query: 68  RLMTIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVEAL 127
           R +   ALAV++     A + V+ +     IT R+  A++DC+EE GD+V+ L  +++ L
Sbjct: 67  RKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKEL 126

Query: 128 K------GNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQL 185
           K        +       +++TW SAALTD+  CMDGF G AM G +K  ++  +V VA+ 
Sbjct: 127 KHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEE 186

[Close]

BLAST of CmoCh16G010990.1 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 4.2e-24
Identity = 63/158 (39.87%), Postives = 95/158 (60.13%), Query Frame = 0


Query: 33  FIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLMTIAALAVALTHTHDASSTVTSLAKSNA 92
           FI+++C+TT YP LC+ SLS  A+ I+ S + +   A+AV L+        V+ L +   
Sbjct: 38  FIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKG 97

Query: 93  ITTREGYALSDCIEEFGDSVEELKMAVEAL------KGNDKTRLDTEDIQTWISAALTDD 152
           +  RE  A+ DC+EE  D+V+ L  +V+ L      K  D+      + QTW SAALTD+
Sbjct: 98  LKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDE 157

Query: 153 NTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLV 185
           NTC DGF+G  M+G +K S++A +++V   TS ALSL+
Sbjct: 158 NTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLI 195

[Close]

BLAST of CmoCh16G010990.1 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 2.7e-23
Identity = 63/157 (40.13%), Postives = 100/157 (63.69%), Query Frame = 0


Query: 33  FIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLMTIAALAVALTHTHDASSTVTSLAKSNA 92
           +IKT+C  T Y  +C +SLSP AS IR + + + + AL + L+    AS  V +++    
Sbjct: 147 YIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGG 206

Query: 93  ITTREGYALSDCIEEFGDSVEELKMAVEAL-----KGNDKTRLDTEDIQTWISAALTDDN 152
           +T  E  A++DC+EE GDSV  L+ ++  L     K + K  +   D++TW+SAALT+D+
Sbjct: 207 LTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDD 266

Query: 153 TCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLV 185
           TCMDGF+   +  +VK+ ++  VV+VA+LTS AL+L+
Sbjct: 267 TCMDGFS--LVKTAVKDLVRRHVVEVARLTSNALALI 301

[Close]

BLAST of CmoCh16G010990.1 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-21
Identity = 68/188 (36.17%), Postives = 105/188 (55.85%), Query Frame = 0


Query: 11  IVFVILINQSYTVA-----TQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLM 70
           ++F+ L+ QS T+A       P +   FI ++C+ T Y  LC+  L+  A  IR +   +
Sbjct: 13  LLFLPLLCQS-TIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQL 72

Query: 71  TIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVEALKGN 130
              ALAV L      +  V  L K+  I  RE  A+ DC+E  GD +E L  ++  LK  
Sbjct: 73  AQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQV 132

Query: 131 DKTRLDTED-------IQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTS 187
            ++  D ++       ++TW+SAALTD+ TC+DGF G  M+G VK +++  VV VA++TS
Sbjct: 133 GRSGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTS 192

The following BLAST results are available for this feature:

BLAST of CmoCh16G010990.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	1.1e-24	40.66	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB37	7.0e-24	36.70	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI74	3.8e-22	40.13	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9STY5	4.2e-21	38.61	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SI72	2.7e-20	38.59	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]

BLAST of CmoCh16G010990.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E6H9	3.3e-93	100.00	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1	[more]
A0A1S3BLP3	4.6e-63	71.88	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1	[more]
A0A0A0L1P3	2.8e-60	71.35	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 S...	[more]
A0A6J1DFW8	3.5e-50	65.03	pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 P...	[more]
A0A5D3D7I0	7.7e-42	77.60	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G...	[more]

BLAST of CmoCh16G010990.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	5.9e-26	39.77	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	5.0e-25	36.70	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	4.2e-24	39.87	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	2.7e-23	40.13	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	1.1e-21	36.17	pectin methylesterase inhibitor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 105..125
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 10..185
None	No IPR available	PANTHER	PTHR31080:SF96	21 KDA PROTEIN-LIKE	coord: 10..185
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 36..184 e-value: 8.54416E-37 score: 123.317
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 29..183 e-value: 3.0E-41 score: 153.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 3..187 e-value: 1.2E-34 score: 117.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 33..183 e-value: 2.0E-35 score: 122.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 28..188 e-value: 1.0E-38 score: 134.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..187

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh16G010990	CmoCh16G010990	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G010990.1:exon:7188	CmoCh16G010990.1:exon:7188	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G010990.1:cds	CmoCh16G010990.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh16G010990.1	CmoCh16G010990.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity