CmoCh16G008330.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G008330.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprolycopene isomerase, chloroplastic
LocationCmo_Chr16: 4382286 .. 4386948 (+)
Sequence length1975
RNA-Seq ExpressionCmoCh16G008330.1
SyntenyCmoCh16G008330.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTTCAAGCGATCGATTTTCGTCTTCATAAATCATAAATATCTCCAATTCTCACATTTCTCTGGTTCAATTGAATCCACTGGAAGCTCCGCCAGTCCGCGGCAATGGACATTTGCTCCAATGTGCTTGGATTCTCTCGGTTTCTTTCCCCTTCTGCTGTTCGAGCTTCAGCCTATCGTCGGCCCATTTTCGCACGAAGCTCCAGCAGTACTCAAGAACCCACTCTCACTACCTCCATATCTCTCAACAACAGAAATGGCTTCTCAGGTCCTTTCTTCGCTCTCTTTTTCTTCCTTTTATCGGCGTTTTTTGCTTTGATCAAATGGTTTTTCATTCATAATCTTCATTCTAGGTTAAATGATTATTTGCAGGGAAGGCAGAGGCGGATGTTGTAGTAATTGGGAGTGGCATTGGGGGGCTTTGCTGTGCAGGTCTGTTAGCTAGATATGGACAAGATGTTCTTGTATTAGAGAGCCATGATTTGCCTGGAGGTGCTGCTCATTCATTTGAGATTAAGGGCTACAAATTCGACTCTGGTCCCTCGTTGTTTTCTGGGTTTCAATCTCGTGGTCCTCAAGCAAATCCACTTGCTCAAGTAAACTCCCTCACTCTGTATCATGCCCCTTATCAATACTGAATAAACACCTAGAATCTCTTCTGTTACATGAAGCAATATAGAGTTCTAATGAAATGGATTTAAGTGTCTTCTGAGTTCGGATAGTCTTGATTATAATTCTTTTCTTGTGATTCATTGAATGGTCATAACATTTGGCATGAAGATAGGGAGATTCAATTTTCATTTGTAACCATCTGAACCCACTACTAGTAGATATTGTTCACTTTGGCCTATTAATTTGTAACAACTCAAGCTCACCGCTTGTAGATATTGTCCGCTTTGACCCGTTACGTATCAGGCCAAAGTAGATAATATCTGCTAGCGGTGGGCTGTTACATATGGTATCAAGTTAGACACCAAACGGTGTGTGAGTAAGGACGTTGGGCCCCTAAGGGAGGTGGATTGTGAGATCCCACATCGATTGGAGAGGGGAATTAAACATTCTTTATAAGGGTGTGGAACCTCTCCCTAACAGACACGTTTTAAAACTGAGGTTGACGGCGATAAGTAACAGGCAAAGCGGACAATATCTGTTAGTAGTGGGTTTGAGCTGTTACAAATGGTATCAACACTAGACACCAGGCGGTGTGCCAATGGGGGTGGATTGTGAGATCCCGCATCGATTGGAAAGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGCAGACACATTTTAAAACCGTGAGGCTTATGGCAATAGTAACGAGCTAAAGTGGACAATATCACACCACTCCTGTATTGCCCACTACCACTAAATGCAGCTTGGCAGACATGAAAAGCCCTTATCATCAAAGCACCCAAAAGTCTTATTTTTCCATCTACTTGCCTGTTAAAATTTTCAGGTTTTAGATGCACTTGGTGAGTCACTTCCTTGTGTCAATTATGATTCATGGATGGTCTACATACCTGAAGGAGAGTTCTTGTCACGCATTGGCCCCACAGAGTTTTTCAAGGTGGATATCAGACAATGAGTTGGTAACCCATTTTACTTCTTGTTCCATTTGCTTTATTTTGATAAGGAAAACATCTTTTTCAGGCACATATTGCCTGTTCAATCACATAGATAAATGTGTATAAGCACCCAAATCAACTACATCCATAAGATTGTGATATCAAAAATTGAAAAGAACAGGAATTAAGCAATGTTTAGTCTGTTACCTTCAGGATCTTGAGACGTATGCAAGTCCAAATGCTGTTCAAGAATGGCAGAAACTATTAGTAAGTAAAATGTAGTCTATTTTCTATTTCCTTCAACAAAATTTTCATTTGGAATATCATTTAGCTTCCATTCGTGTATACAGGAAACCATACTTCCATTGTCTGCTGCAGCAATGGCTCTACCACCGCTATCAATTCGAGGCAATTGGGGAGTTCTTTCAACAGCTGCAGCAAGATATGCTCCTTCTCTCTTGAAATCCTTCATTCAGATGGGACCTCAGGGTATTCTTGGTTCGACCAAGCTTCTCAGACCATTCTCAGAAATCATCGACTCGTTGGAACTTAACGACCCTTTCATTCGAAATTGGTTGGATCTGTTGGCTTTCTTACTTGCTGGAGTAAAAACAAATGGCATATTATCAGCAGAGATGGTAAATATTTTTATGAAGCTTTAGAAAAACTAAAATCTGAGTCTTGTCAGTTTCTGTATTACTGCATCTGGTATTAGACATGCATGTTACTGAGAACACTGTAGGTAAAAACATTCTGTTTTTATTCTCATTTTGCCTAGATATAGTAGCATAAGTCATAACGGTGAGATCTCACATCGGTTGGAGAGGGGAACGAAACATTTCTTATAAGGGTGTGGAAACCTCTCCTTAGCAAACGCGTTTTAAAACCATGAGACTGACAGCGATACGTAACGAGCCAGAGCAGACAATATCTGCTAGTGGTGGGCTTGGACTGTTACAAATGGTATTAGAATCAGACACCGGATGGTGTGCTAGCGAGGATGCTGGGTCCTCAAGGGGGGTGGATTGTAAGATCTTACCTCGGTTGGAGAGGGAAATAAAGCATTCCTTATATGAGTGTGGAAACCTCTCCTTAGCAGGCGCGTTTTAAAACCGTGAGGCTAAGGGCGATGTGTAACGGGCCAAAGCGGATAATATCTGCTAACGGTAGGCTTGGGTTGTTACAATAACTTTCTTACATTTTTGGGCCCTCATGTTTGAATGCCACCTAGGATAACAAATGCATTGATTTTTCTTCTACATTTCAGATTTATATGTTTGCAGAATGGTATAAGCCAGGTTCCTGTCTCGAATATCCTCTTCATGGAAGTGGGGCAGTTGTTAACGCCCTTGTTCAAGGCCTGCAAAAATTTGGAGGGCGTATCTCTCTTGGAAGTCATGTTCAGAACATTATAGTCGAGAACGATCGAGCCGTTGGGGTCAAGCTAAAGAGCGGTCAAGTAAATTCTATGGAATCAATCACAGTCATTGTTAAGGAACATCAACAGTATCCTCCATTTTGTAATCCTCTAATGTCATGATTGAATATGTTGCAGTTTATACGTGCAAAGAAGGCTGTTGTGAGCAATGCATCCATGTGGGATACACTGGCCTTGTTACCTGAAAGTGTTGTTCCTAAATCATATCGTGATGGTGTAAATGCAATGCCACAATGTGAATCGTTTATGCATCTTCACTTAGGCTTTGACGCAGAGGTGAGTGATGTTAGCAATACCATTTTCTTCCTTTTTCCTATGAGACTAAATCCTTCTACAAGGAGAGGATAGTGCTTAACAATTCTCGTTCCATATGGATAGCTATAATGGTTTTCATGTAAAAAGTGTTAGGAACCACGACCCTCCACAATAGTATGATATTGTCCACTTTGAGCATAAGCTCTCATGGCTTCGCTTTTGGTTTCCCCAAAATGCCTCATACCAATGGAGATGTATTCCTAACCCATGATCAACCCCTTAATTAGCTTACGTGGGACTCCTCTCCCACCAATCCTCAACAATACTCCCCTCGAACAAAGTACACTGTAGAGTCTCCCCTGAGGCTTATGGAGCCCTCGAACAACCTTCCTTTAATCGAGCCTCAACTCCTTCTCTAGTGTACTCGAATAAAGTACACTCTTTGTTCGACACTTGAGGATTCTATTGACATGACTAAGTTAAAGGCATGACTCTAATACCATGTTAGAAACCACAACCCTCCACATATTTAGCTTTATTACAGTTGGGTGTTGCTCCCTAGAAAATTCTGAGTTCAAGATGTGTGAAGTCTGTGCATGTGTTTTTCAGGGTATTCGTGAAGACCTGGGAATTCATCATATAGTTGTAAACGATTGGGATAGAGGAGTGGATGCAGATCAAAACGTGGTTTTGATATCTGTACCAAGCGTTCTTAGTCCAGATCTAGCACCCCCTGGAAAACATGTGTTACATGCATACACACCAGGAACAGAACCATATACTCTTTGGGAAGGACTTGACCGTAGGAGCTCTGAGTACAAAAATCTCAAGGCTCAAAGATCTGAGGTAAGTTCTTCAACTTTTCTTAGTCTTTCTTATGTAATGCTTCGAGTTTCAAACACGAATATCGAAGATTTTAAGAACCTAACCGAAACGAGTTTAAACCCGAACTATTTTGGTTGAAAAGGTGATGTGGAGGGCAGTTGAGAGAGCCCTTGGGCCAGGTTTCAAGAGAGAAAAGTGTGAGGTGAAGTTGGTGGGAAGTCCATTGACGCATCAAAGGTTTCTTAGGAGGAACAGAGGAACATATGGGCCAGCAATTGAAGCAGGGAAAGCCACATTTCCTGGGCATTCGACTCCCATCCCTCAGCTTTACTGCTGTGGGGATTCTACTTTCCCTGGCATTGGAGTCCCTGCAGTGGCAGCCAGTGGTGCCATTGTTGCCAATTCTTTGGTCTCTACTTCCCAACACTCTCAGCTTCTTGATGCAATTGGAATCTGATTTCATTTTGACATCTTTTTCATTCCCTTATCCTGCACTTACCATCTTCTGTAAATTTGTATTATAACAATTGGATTAGATATAAAGTTTTATTCTCTGTGTTTGGTCA

mRNA sequence

ACCTTCAAGCGATCGATTTTCGTCTTCATAAATCATAAATATCTCCAATTCTCACATTTCTCTGGTTCAATTGAATCCACTGGAAGCTCCGCCAGTCCGCGGCAATGGACATTTGCTCCAATGTGCTTGGATTCTCTCGGTTTCTTTCCCCTTCTGCTGTTCGAGCTTCAGCCTATCGTCGGCCCATTTTCGCACGAAGCTCCAGCAGTACTCAAGAACCCACTCTCACTACCTCCATATCTCTCAACAACAGAAATGGCTTCTCAGGGAAGGCAGAGGCGGATGTTGTAGTAATTGGGAGTGGCATTGGGGGGCTTTGCTGTGCAGGTCTGTTAGCTAGATATGGACAAGATGTTCTTGTATTAGAGAGCCATGATTTGCCTGGAGGTGCTGCTCATTCATTTGAGATTAAGGGCTACAAATTCGACTCTGGTCCCTCGTTGTTTTCTGGGTTTCAATCTCGTGGTCCTCAAGCAAATCCACTTGCTCAAGTTTTAGATGCACTTGGTGAGTCACTTCCTTGTGTCAATTATGATTCATGGATGGTCTACATACCTGAAGGAGAGTTCTTGTCACGCATTGGCCCCACAGAGTTTTTCAAGGTGGATATCAGACAATGAGTTGGATCTTGAGACGTATGCAAGTCCAAATGCTGTTCAAGAATGGCAGAAACTATTAGAAACCATACTTCCATTGTCTGCTGCAGCAATGGCTCTACCACCGCTATCAATTCGAGGCAATTGGGGAGTTCTTTCAACAGCTGCAGCAAGATATGCTCCTTCTCTCTTGAAATCCTTCATTCAGATGGGACCTCAGGGTATTCTTGGTTCGACCAAGCTTCTCAGACCATTCTCAGAAATCATCGACTCGTTGGAACTTAACGACCCTTTCATTCGAAATTGGTTGGATCTGTTGGCTTTCTTACTTGCTGGAGTAAAAACAAATGGCATATTATCAGCAGAGATGATTTATATGTTTGCAGAATGGTATAAGCCAGGTTCCTGTCTCGAATATCCTCTTCATGGAAGTGGGGCAGTTGTTAACGCCCTTGTTCAAGGCCTGCAAAAATTTGGAGGGCGTATCTCTCTTGGAAGTCATGTTCAGAACATTATAGTCGAGAACGATCGAGCCGTTGGGGTCAAGCTAAAGAGCGGTCAATTTATACGTGCAAAGAAGGCTGTTGTGAGCAATGCATCCATGTGGGATACACTGGCCTTGTTACCTGAAAGTGTTGTTCCTAAATCATATCGTGATGGTGTAAATGCAATGCCACAATGTGAATCGTTTATGCATCTTCACTTAGGCTTTGACGCAGAGGGTATTCGTGAAGACCTGGGAATTCATCATATAGTTGTAAACGATTGGGATAGAGGAGTGGATGCAGATCAAAACGTGGTTTTGATATCTGTACCAAGCGTTCTTAGTCCAGATCTAGCACCCCCTGGAAAACATGTGTTACATGCATACACACCAGGAACAGAACCATATACTCTTTGGGAAGGACTTGACCGTAGGAGCTCTGAGTACAAAAATCTCAAGGCTCAAAGATCTGAGATGTGGAGGGCAGTTGAGAGAGCCCTTGGGCCAGGTTTCAAGAGAGAAAAGTGTGAGGTGAAGTTGGTGGGAAGTCCATTGACGCATCAAAGGTTTCTTAGGAGGAACAGAGGAACATATGGGCCAGCAATTGAAGCAGGGAAAGCCACATTTCCTGGGCATTCGACTCCCATCCCTCAGCTTTACTGCTGTGGGGATTCTACTTTCCCTGGCATTGGAGTCCCTGCAGTGGCAGCCAGTGGTGCCATTGTTGCCAATTCTTTGGTCTCTACTTCCCAACACTCTCAGCTTCTTGATGCAATTGGAATCTGATTTCATTTTGACATCTTTTTCATTCCCTTATCCTGCACTTACCATCTTCTGTAAATTTGTATTATAACAATTGGATTAGATATAAAGTTTTATTCTCTGTGTTTGGTCA

Coding sequence (CDS)

ATGCACTTGGTGAGTCACTTCCTTGTGTCAATTATGATTCATGGATGGTCTACATACCTGAAGGAGAGTTCTTGTCACGCATTGGCCCCACAGAGTTTTTCAAGGTGGATATCAGACAATGAGTTGGATCTTGAGACGTATGCAAGTCCAAATGCTGTTCAAGAATGGCAGAAACTATTAGAAACCATACTTCCATTGTCTGCTGCAGCAATGGCTCTACCACCGCTATCAATTCGAGGCAATTGGGGAGTTCTTTCAACAGCTGCAGCAAGATATGCTCCTTCTCTCTTGAAATCCTTCATTCAGATGGGACCTCAGGGTATTCTTGGTTCGACCAAGCTTCTCAGACCATTCTCAGAAATCATCGACTCGTTGGAACTTAACGACCCTTTCATTCGAAATTGGTTGGATCTGTTGGCTTTCTTACTTGCTGGAGTAAAAACAAATGGCATATTATCAGCAGAGATGATTTATATGTTTGCAGAATGGTATAAGCCAGGTTCCTGTCTCGAATATCCTCTTCATGGAAGTGGGGCAGTTGTTAACGCCCTTGTTCAAGGCCTGCAAAAATTTGGAGGGCGTATCTCTCTTGGAAGTCATGTTCAGAACATTATAGTCGAGAACGATCGAGCCGTTGGGGTCAAGCTAAAGAGCGGTCAATTTATACGTGCAAAGAAGGCTGTTGTGAGCAATGCATCCATGTGGGATACACTGGCCTTGTTACCTGAAAGTGTTGTTCCTAAATCATATCGTGATGGTGTAAATGCAATGCCACAATGTGAATCGTTTATGCATCTTCACTTAGGCTTTGACGCAGAGGGTATTCGTGAAGACCTGGGAATTCATCATATAGTTGTAAACGATTGGGATAGAGGAGTGGATGCAGATCAAAACGTGGTTTTGATATCTGTACCAAGCGTTCTTAGTCCAGATCTAGCACCCCCTGGAAAACATGTGTTACATGCATACACACCAGGAACAGAACCATATACTCTTTGGGAAGGACTTGACCGTAGGAGCTCTGAGTACAAAAATCTCAAGGCTCAAAGATCTGAGATGTGGAGGGCAGTTGAGAGAGCCCTTGGGCCAGGTTTCAAGAGAGAAAAGTGTGAGGTGAAGTTGGTGGGAAGTCCATTGACGCATCAAAGGTTTCTTAGGAGGAACAGAGGAACATATGGGCCAGCAATTGAAGCAGGGAAAGCCACATTTCCTGGGCATTCGACTCCCATCCCTCAGCTTTACTGCTGTGGGGATTCTACTTTCCCTGGCATTGGAGTCCCTGCAGTGGCAGCCAGTGGTGCCATTGTTGCCAATTCTTTGGTCTCTACTTCCCAACACTCTCAGCTTCTTGATGCAATTGGAATCTGA

Protein sequence

MHLVSHFLVSIMIHGWSTYLKESSCHALAPQSFSRWISDNELDLETYASPNAVQEWQKLLETILPLSAAAMALPPLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRNWLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGVNAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAPPGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEMWRAVERALGPGFKREKCEVKLVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASGAIVANSLVSTSQHSQLLDAIGI
Homology
BLAST of CmoCh16G008330.1 vs. ExPASy Swiss-Prot
Match: Q2VEX9 (Prolycopene isomerase, chloroplastic OS=Daucus carota OX=4039 GN=CRTISO PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.9e-44
Identity = 113/335 (33.73%), Postives = 181/335 (54.03%), Query Frame = 0

Query: 127 LNDPFIRNWLDLLAFLLAGVKT--NGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNAL 186
           + DP + +++D   F+++ V      +++A M+     +      + YP+ G G +  +L
Sbjct: 277 IKDPEVLSFIDAECFIVSTVNALKTPMINASMVLCDRHY----GGINYPVGGVGGIAKSL 336

Query: 187 VQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLA-LLPE 246
            +GL   G  I   ++V++IIVEN +AVGV+L +G  I AK  ++SNA+ WDT   LL +
Sbjct: 337 AKGLVDQGSEIQYKANVKSIIVENGKAVGVRLANGNEIFAKN-IISNATRWDTFGKLLKQ 396

Query: 247 SVVPKSYRDGVNAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLIS 306
             +PK   +      +  SF+ +HLG  ++ +  D   HH V+ D    ++     + +S
Sbjct: 397 DELPKEEENFQKLYIKAPSFLSIHLGVKSDVLPPDTDCHHFVLEDDWSNLEVPYGSIFLS 456

Query: 307 VPSVLSPDLAPPGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEMWRAVERALGP 366
           +P+VL   LAP G H+LH +T  T     W+G+ ++  E K  +    E+   +E+ L P
Sbjct: 457 IPTVLDSSLAPEGNHILHIFT--TSSIEDWQGMSQKDYE-KKKELVADEIISRLEKKLFP 516

Query: 367 GFKREKCEVKLVGSPLTHQRFLRRNRGTYGPAIEA---GKATFPGHSTPIPQLYCCGDST 426
           G  +    +K VG+P TH+R+L R+ GTYGP  +    G    P ++T I  LYC GDS 
Sbjct: 517 GL-QSSIVLKEVGTPKTHRRYLARDSGTYGPMPQGTPKGLLGMPFNTTAIDGLYCVGDSC 576

Query: 427 FPGIGVPAVAASGAIVANSL---VSTSQHSQLLDA 453
           FPG GV AVA SG + A+ +   +   Q S +LDA
Sbjct: 577 FPGQGVIAVAFSGVMCAHRVAADLGLEQKSPILDA 602

BLAST of CmoCh16G008330.1 vs. ExPASy Swiss-Prot
Match: Q52QW3 (Prolycopene isomerase 1, chloroplastic OS=Oncidium hybrid cultivar OX=141207 GN=CRTISO1 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.2e-42
Identity = 110/323 (34.06%), Postives = 175/323 (54.18%), Query Frame = 0

Query: 127 LNDPFIRNWLDLLAFLLAGVKT--NGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNAL 186
           + DP + +++D   F+++ VK     +++A M+     +      + YP+ G G +  AL
Sbjct: 249 IKDPQLLSFIDAECFIVSTVKALHTPMINASMVLCDRHF----GGINYPVGGVGGIAKAL 308

Query: 187 VQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLA-LLPE 246
             GL   G ++   ++V  I++++ +AVGVKL +G+   A K V+SNA+ WDT   LL  
Sbjct: 309 ANGLVNKGSKLLYKANVTKILLKDGKAVGVKLSNGREFFA-KTVISNATRWDTFGKLLKV 368

Query: 247 SVVPKSYRDGVNAMPQCESFMHLHLGFDAEGIREDLGIHHIVVND-WDRGVDADQNVVLI 306
             +P+  ++      +  SF+ +H+G  A  +  D   HH ++ D W R ++     + +
Sbjct: 369 EDIPQEEKNFKKIYLKAPSFLSIHMGVKAFVLPPDTDCHHFILEDNWGR-LELPYGSIFL 428

Query: 307 SVPSVLSPDLAPPGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEMWRAVERALG 366
           S+P+VL P LAP G H+ H +T  T     WEGL  +  E K  +    E+   +E+ L 
Sbjct: 429 SIPTVLDPSLAPEGHHIFHIFT--TSSIENWEGLSHKEYEEKK-ELVADEIITRLEKKLF 488

Query: 367 PGFKREKCEVKLVGSPLTHQRFLRRNRGTYGP---AIEAGKATFPGHSTPIPQLYCCGDS 426
           PG K +   +K VG+P TH+RFL R+ GTYGP    +  G    P ++T I  LYC GDS
Sbjct: 489 PGLK-DSVVLKEVGTPKTHRRFLARDSGTYGPMPRKVPKGLLGMPFNTTAINGLYCVGDS 548

Query: 427 TFPGIGVPAVAASGAIVANSLVS 443
            FPG GV AVA SG + A+ + +
Sbjct: 549 CFPGQGVIAVAFSGVMCAHRVAA 561

BLAST of CmoCh16G008330.1 vs. ExPASy Swiss-Prot
Match: Q52QW2 (Prolycopene isomerase 2, chloroplastic OS=Oncidium hybrid cultivar OX=141207 GN=CRTISO2 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.2e-42
Identity = 110/323 (34.06%), Postives = 175/323 (54.18%), Query Frame = 0

Query: 127 LNDPFIRNWLDLLAFLLAGVKT--NGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNAL 186
           + DP + +++D   F+++ VK     +++A M+     +      + YP+ G G +  AL
Sbjct: 249 IKDPQLLSFIDAECFIVSTVKALHTPMINASMVLCDRHF----GGINYPVGGVGGIAKAL 308

Query: 187 VQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLA-LLPE 246
             GL   G ++   ++V  I++++ +AVGVKL +G+   A K V+SNA+ WDT   LL  
Sbjct: 309 ANGLVNKGSKLLYKANVTKILLKDGKAVGVKLSNGREFFA-KTVISNATRWDTFGKLLKV 368

Query: 247 SVVPKSYRDGVNAMPQCESFMHLHLGFDAEGIREDLGIHHIVVND-WDRGVDADQNVVLI 306
             +P+  ++      +  SF+ +H+G  A  +  D   HH ++ D W R ++     + +
Sbjct: 369 EDIPQEEKNFKKIYLKAPSFLSIHMGVKAFVLPPDTDCHHFILEDNWGR-LELPYGSIFL 428

Query: 307 SVPSVLSPDLAPPGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEMWRAVERALG 366
           S+P+VL P LAP G H+ H +T  T     WEGL  +  E K  +    E+   +E+ L 
Sbjct: 429 SIPTVLDPSLAPEGHHIFHIFT--TSSIENWEGLSHKEYEEKK-ELVADEIITRLEKKLF 488

Query: 367 PGFKREKCEVKLVGSPLTHQRFLRRNRGTYGP---AIEAGKATFPGHSTPIPQLYCCGDS 426
           PG K +   +K VG+P TH+RFL R+ GTYGP    +  G    P ++T I  LYC GDS
Sbjct: 489 PGLK-DSVVLKEVGTPKTHRRFLARDSGTYGPMPRKVPKGLLGMPFNTTAINGLYCVGDS 548

Query: 427 TFPGIGVPAVAASGAIVANSLVS 443
            FPG GV AVA SG + A+ + +
Sbjct: 549 CFPGQGVIAVAFSGVMCAHRVAA 561

BLAST of CmoCh16G008330.1 vs. ExPASy Swiss-Prot
Match: Q8S4R4 (Prolycopene isomerase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=CRTISO PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.2e-42
Identity = 113/335 (33.73%), Postives = 176/335 (52.54%), Query Frame = 0

Query: 127 LNDPFIRNWLDLLAFLLAGVKT--NGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNAL 186
           + DP + +++D   F+++ V      +++A M+     +      + YP+ G G +  +L
Sbjct: 277 IRDPGLLSFIDAECFIVSTVNALQTPMINASMVLCDRHF----GGINYPVGGVGEIAKSL 336

Query: 187 VQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLA-LLPE 246
            +GL   G +I   ++V +II++N +AVGVKL  G+   A K +VSNA+ WDT   LL  
Sbjct: 337 AKGLDDHGSQILYRANVTSIILDNGKAVGVKLSDGRKFYA-KTIVSNATRWDTFGKLLKA 396

Query: 247 SVVPKSYRDGVNAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLIS 306
             +PK   +   A  +  SF+ +H+G  A+ +  D   HH V+ D    ++     + +S
Sbjct: 397 ENLPKEEENFQKAYVKAPSFLSIHMGVKADVLPPDTDCHHFVLEDDWTNLEKPYGSIFLS 456

Query: 307 VPSVLSPDLAPPGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEMWRAVERALGP 366
           +P+VL   LAP G H+LH +T  T     WEGL  +  E K  +     +   +E+ L P
Sbjct: 457 IPTVLDSSLAPEGHHILHIFT--TSSIEDWEGLSPKDYEAKK-EVVAERIISRLEKTLFP 516

Query: 367 GFKREKCEVKLVGSPLTHQRFLRRNRGTYGP---AIEAGKATFPGHSTPIPQLYCCGDST 426
           G K      K VG+P TH+R+L R+ GTYGP       G    P ++T I  LYC GDS 
Sbjct: 517 GLK-SSILFKEVGTPKTHRRYLARDSGTYGPMPRGTPKGLLGMPFNTTAIDGLYCVGDSC 576

Query: 427 FPGIGVPAVAASGAIVANSLVST---SQHSQLLDA 453
           FPG GV AVA SG + A+ + +     + S +LD+
Sbjct: 577 FPGQGVIAVAFSGVMCAHRVAADLGFEKKSDVLDS 602

BLAST of CmoCh16G008330.1 vs. ExPASy Swiss-Prot
Match: Q9M9Y8 (Prolycopene isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRTISO PE=1 SV=2)

HSP 1 Score: 175.3 bits (443), Expect = 1.6e-42
Identity = 109/334 (32.63%), Postives = 179/334 (53.59%), Query Frame = 0

Query: 127 LNDPFIRNWLDLLAFLLAGVKT--NGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNAL 186
           + DP + +++D   F+++ V      +++A M+     +      + YP+ G G +  +L
Sbjct: 257 IKDPQLLSFIDAECFIVSTVNALQTPMINASMVLCDRHY----GGINYPVGGVGGIAKSL 316

Query: 187 VQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLA-LLPE 246
            +GL   G  I   ++V++II+++ +AVGV+L  G+   A K ++SNA+ WDT   LL  
Sbjct: 317 AEGLVDQGSEIQYKANVKSIILDHGKAVGVRLADGREFFA-KTIISNATRWDTFGKLLKG 376

Query: 247 SVVPKSYRDGVNAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLIS 306
             +PK   +      +  SF+ +H+G  AE +  D   HH V+ D  + ++     + +S
Sbjct: 377 EKLPKEEENFQKVYVKAPSFLSIHMGVKAEVLPPDTDCHHFVLEDDWKNLEEPYGSIFLS 436

Query: 307 VPSVLSPDLAPPGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEMWRAVERALGP 366
           +P++L   LAP G+H+LH +T  T     WEGL  +  E K  +   + + + +E+ L P
Sbjct: 437 IPTILDSSLAPDGRHILHIFT--TSSIEDWEGLPPKEYEAKK-EDVAARIIQRLEKKLFP 496

Query: 367 GFKREKCEVKLVGSPLTHQRFLRRNRGTYGP---AIEAGKATFPGHSTPIPQLYCCGDST 426
           G        K VG+P TH+RFL R++GTYGP       G    P ++T I  LYC GDS 
Sbjct: 497 GLS-SSITFKEVGTPRTHRRFLARDKGTYGPMPRGTPKGLLGMPFNTTAIDGLYCVGDSC 556

Query: 427 FPGIGVPAVAASGAIVANSL---VSTSQHSQLLD 452
           FPG GV AVA SG + A+ +   +   + S++LD
Sbjct: 557 FPGQGVIAVAFSGVMCAHRVAADIGLEKKSRVLD 581

BLAST of CmoCh16G008330.1 vs. ExPASy TrEMBL
Match: A0A1S4DZ57 (prolycopene isomerase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493319 PE=4 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 7.2e-243
Identity = 425/456 (93.20%), Postives = 436/456 (95.61%), Query Frame = 0

Query: 1   MHLVSHFLVSIMIHGWSTYLKESSCHALAPQSFSRWISDNELDLETYASPNAVQEWQKLL 60
           MHLVS FLV IMIHGWSTYLKE+  HAL PQSFS  +     DLETYASPNAVQEWQKLL
Sbjct: 53  MHLVSRFLVLIMIHGWSTYLKENFYHALVPQSFSSLLCLQ--DLETYASPNAVQEWQKLL 112

Query: 61  ETILPLSAAAMALPPLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSE 120
           ETILPLSAAAMALPPLSIRG+WGVLSTAAARYAPSLLKSFIQMGPQGI+GSTKLLRPFSE
Sbjct: 113 ETILPLSAAAMALPPLSIRGDWGVLSTAAARYAPSLLKSFIQMGPQGIIGSTKLLRPFSE 172

Query: 121 IIDSLELNDPFIRNWLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAV 180
           IIDSLEL DPFIRNWLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAV
Sbjct: 173 IIDSLELKDPFIRNWLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAV 232

Query: 181 VNALVQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLAL 240
           VNALVQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMW+TLAL
Sbjct: 233 VNALVQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWNTLAL 292

Query: 241 LPESVVPKSYRDGVNAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVV 300
           LPE VVPKSYRD VN MPQCESFMHLHLGFDAEGIR+DLGIHHIVVNDWDRGVDADQNVV
Sbjct: 293 LPEGVVPKSYRDSVNTMPQCESFMHLHLGFDAEGIRDDLGIHHIVVNDWDRGVDADQNVV 352

Query: 301 LISVPSVLSPDLAPPGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVER 360
           LISVPSVLSPDLAPPGKHVLHAYTPGTEPYTLW+GLDRRSSEYKNLKA+RSE MWRAVER
Sbjct: 353 LISVPSVLSPDLAPPGKHVLHAYTPGTEPYTLWKGLDRRSSEYKNLKAERSEVMWRAVER 412

Query: 361 ALGPGFKREKCEVKLVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDS 420
           A+G GFKREKCEVKLVGSPLTH+RFLRR RGTYGPAIEAGK +FPGHSTPIPQLYCCGDS
Sbjct: 413 AVGAGFKREKCEVKLVGSPLTHERFLRRKRGTYGPAIEAGKGSFPGHSTPIPQLYCCGDS 472

Query: 421 TFPGIGVPAVAASGAIVANSLVSTSQHSQLLDAIGI 456
           TFPGIGVPAVAASGAIVANSLVSTSQHSQLLDAIGI
Sbjct: 473 TFPGIGVPAVAASGAIVANSLVSTSQHSQLLDAIGI 506

BLAST of CmoCh16G008330.1 vs. ExPASy TrEMBL
Match: A0A6J1ENI9 (prolycopene isomerase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435961 PE=4 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.0e-237
Identity = 418/442 (94.57%), Postives = 421/442 (95.25%), Query Frame = 0

Query: 16  WSTYLKESS-CHALAPQSFSRWISDNELDLETYASPNAVQEWQKLLETILPLSAAAMALP 75
           W  Y+ E      + P  F +       DLETYASPNAVQEWQKLLETILPLSAAAMALP
Sbjct: 146 WMVYIPEGEFLSRIGPTEFFK-------DLETYASPNAVQEWQKLLETILPLSAAAMALP 205

Query: 76  PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 135
           PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN
Sbjct: 206 PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 265

Query: 136 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 195
           WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR
Sbjct: 266 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 325

Query: 196 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 255
           ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV
Sbjct: 326 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 385

Query: 256 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 315
           NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP
Sbjct: 386 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 445

Query: 316 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVERALGPGFKREKCEVK 375
           PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE MWRAVERALGPGFKREKCEVK
Sbjct: 446 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEVMWRAVERALGPGFKREKCEVK 505

Query: 376 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 435
           LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG
Sbjct: 506 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 565

Query: 436 AIVANSLVSTSQHSQLLDAIGI 456
           AIVANSLVSTSQHSQLLDAIGI
Sbjct: 566 AIVANSLVSTSQHSQLLDAIGI 580

BLAST of CmoCh16G008330.1 vs. ExPASy TrEMBL
Match: A0A6J1JAD0 (prolycopene isomerase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111482703 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 4.2e-235
Identity = 414/442 (93.67%), Postives = 419/442 (94.80%), Query Frame = 0

Query: 16  WSTYLKESS-CHALAPQSFSRWISDNELDLETYASPNAVQEWQKLLETILPLSAAAMALP 75
           W  Y+ E      + P  F +       DLETYASPNAVQEWQKLLETILPLSAAAMALP
Sbjct: 146 WMVYIPEGEFLSRIGPTEFFK-------DLETYASPNAVQEWQKLLETILPLSAAAMALP 205

Query: 76  PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 135
           PLSIRG+WGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN
Sbjct: 206 PLSIRGDWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 265

Query: 136 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 195
           WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR
Sbjct: 266 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 325

Query: 196 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 255
           ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV
Sbjct: 326 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 385

Query: 256 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 315
           NAMPQCESFMHLHLGFDA GIREDLGIHHIVVNDW+RGVDADQNVVLISVPSVLSPDLAP
Sbjct: 386 NAMPQCESFMHLHLGFDAVGIREDLGIHHIVVNDWERGVDADQNVVLISVPSVLSPDLAP 445

Query: 316 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVERALGPGFKREKCEVK 375
           PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE MWRAVERALGPGFKREKCEVK
Sbjct: 446 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEVMWRAVERALGPGFKREKCEVK 505

Query: 376 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 435
           LVGSPLTHQRFLRRNRGTYGPAIEAGK TFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG
Sbjct: 506 LVGSPLTHQRFLRRNRGTYGPAIEAGKGTFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 565

Query: 436 AIVANSLVSTSQHSQLLDAIGI 456
           AIVANSLVSTSQHSQLLDAIGI
Sbjct: 566 AIVANSLVSTSQHSQLLDAIGI 580

BLAST of CmoCh16G008330.1 vs. ExPASy TrEMBL
Match: A0A0A0L3B1 (Amino_oxidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G110060 PE=4 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 9.1e-230
Identity = 403/442 (91.18%), Postives = 414/442 (93.67%), Query Frame = 0

Query: 16  WSTYLKESS-CHALAPQSFSRWISDNELDLETYASPNAVQEWQKLLETILPLSAAAMALP 75
           W  Y+ E      + P  F +       DLETYASPNAVQEWQKLLETILPLSAAAMALP
Sbjct: 148 WMVYIPEGEFLSRIGPTEFFK-------DLETYASPNAVQEWQKLLETILPLSAAAMALP 207

Query: 76  PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 135
           PLSIRG+WGVLSTAAARYAPSLLKSFI+MGPQGI+GSTKLLRPFSEIIDSLEL DPFIRN
Sbjct: 208 PLSIRGDWGVLSTAAARYAPSLLKSFIEMGPQGIIGSTKLLRPFSEIIDSLELKDPFIRN 267

Query: 136 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 195
           WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR
Sbjct: 268 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 327

Query: 196 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 255
           ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMW+TLALLPE VVPKSYRD V
Sbjct: 328 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWNTLALLPEDVVPKSYRDSV 387

Query: 256 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 315
           N MPQCESFMHLHLGFDAEGIR+DLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP
Sbjct: 388 NTMPQCESFMHLHLGFDAEGIRDDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 447

Query: 316 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVERALGPGFKREKCEVK 375
           PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKA+RSE MWRAVERALG GFKREKCEVK
Sbjct: 448 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAERSEVMWRAVERALGAGFKREKCEVK 507

Query: 376 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 435
           LVGSPLTH+RFLRR RGTYGPAIEAGK +FPGHSTPIPQLYCCGDSTFPGIGVPAVAASG
Sbjct: 508 LVGSPLTHERFLRRKRGTYGPAIEAGKGSFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 567

Query: 436 AIVANSLVSTSQHSQLLDAIGI 456
           AIVANSLVSTSQHSQLLDAIGI
Sbjct: 568 AIVANSLVSTSQHSQLLDAIGI 582

BLAST of CmoCh16G008330.1 vs. ExPASy TrEMBL
Match: A0A6J1C5F2 (prolycopene isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111008423 PE=4 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.5e-229
Identity = 404/442 (91.40%), Postives = 412/442 (93.21%), Query Frame = 0

Query: 16  WSTYLKESS-CHALAPQSFSRWISDNELDLETYASPNAVQEWQKLLETILPLSAAAMALP 75
           W  Y+ E      + P  F +       DLE YASPNAVQEWQKLLETILPLSA AMALP
Sbjct: 148 WMVYIPEGEFLSRIGPTEFFK-------DLEKYASPNAVQEWQKLLETILPLSAVAMALP 207

Query: 76  PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 135
           PLSIRG+WGVLSTAAARYAPSLLKSFIQMGPQGI+GSTKLLRPFSEIIDSLEL+DPFIRN
Sbjct: 208 PLSIRGDWGVLSTAAARYAPSLLKSFIQMGPQGIIGSTKLLRPFSEIIDSLELSDPFIRN 267

Query: 136 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 195
           WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGS LEYPLHGSGAVVNALVQGLQKFGGR
Sbjct: 268 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSSLEYPLHGSGAVVNALVQGLQKFGGR 327

Query: 196 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 255
           ISLGSHVQNIIVENDRAVGVKLKSGQF+RAKKAVVSNASMWDTLALLPE  VPKSYRD V
Sbjct: 328 ISLGSHVQNIIVENDRAVGVKLKSGQFVRAKKAVVSNASMWDTLALLPEGAVPKSYRDSV 387

Query: 256 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 315
           N MPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP
Sbjct: 388 NTMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 447

Query: 316 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVERALGPGFKREKCEVK 375
           PGKHVLHAYTPGTEPY LWEGLDRRSSEYKNLKAQRSE MWRAVERALG GFKREKCEVK
Sbjct: 448 PGKHVLHAYTPGTEPYALWEGLDRRSSEYKNLKAQRSEVMWRAVERALGEGFKREKCEVK 507

Query: 376 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 435
           LVGSPLTHQRFLRRNRGTYGPAIEAGKA+FPGHSTPIPQLYCCGDSTFPGIGVPAVAASG
Sbjct: 508 LVGSPLTHQRFLRRNRGTYGPAIEAGKASFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 567

Query: 436 AIVANSLVSTSQHSQLLDAIGI 456
           AIVANSLVSTSQHSQLLDAIGI
Sbjct: 568 AIVANSLVSTSQHSQLLDAIGI 582

BLAST of CmoCh16G008330.1 vs. NCBI nr
Match: XP_016901253.1 (PREDICTED: prolycopene isomerase, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 849.4 bits (2193), Expect = 1.5e-242
Identity = 425/456 (93.20%), Postives = 436/456 (95.61%), Query Frame = 0

Query: 1   MHLVSHFLVSIMIHGWSTYLKESSCHALAPQSFSRWISDNELDLETYASPNAVQEWQKLL 60
           MHLVS FLV IMIHGWSTYLKE+  HAL PQSFS  +     DLETYASPNAVQEWQKLL
Sbjct: 53  MHLVSRFLVLIMIHGWSTYLKENFYHALVPQSFSSLLCLQ--DLETYASPNAVQEWQKLL 112

Query: 61  ETILPLSAAAMALPPLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSE 120
           ETILPLSAAAMALPPLSIRG+WGVLSTAAARYAPSLLKSFIQMGPQGI+GSTKLLRPFSE
Sbjct: 113 ETILPLSAAAMALPPLSIRGDWGVLSTAAARYAPSLLKSFIQMGPQGIIGSTKLLRPFSE 172

Query: 121 IIDSLELNDPFIRNWLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAV 180
           IIDSLEL DPFIRNWLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAV
Sbjct: 173 IIDSLELKDPFIRNWLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAV 232

Query: 181 VNALVQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLAL 240
           VNALVQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMW+TLAL
Sbjct: 233 VNALVQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWNTLAL 292

Query: 241 LPESVVPKSYRDGVNAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVV 300
           LPE VVPKSYRD VN MPQCESFMHLHLGFDAEGIR+DLGIHHIVVNDWDRGVDADQNVV
Sbjct: 293 LPEGVVPKSYRDSVNTMPQCESFMHLHLGFDAEGIRDDLGIHHIVVNDWDRGVDADQNVV 352

Query: 301 LISVPSVLSPDLAPPGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVER 360
           LISVPSVLSPDLAPPGKHVLHAYTPGTEPYTLW+GLDRRSSEYKNLKA+RSE MWRAVER
Sbjct: 353 LISVPSVLSPDLAPPGKHVLHAYTPGTEPYTLWKGLDRRSSEYKNLKAERSEVMWRAVER 412

Query: 361 ALGPGFKREKCEVKLVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDS 420
           A+G GFKREKCEVKLVGSPLTH+RFLRR RGTYGPAIEAGK +FPGHSTPIPQLYCCGDS
Sbjct: 413 AVGAGFKREKCEVKLVGSPLTHERFLRRKRGTYGPAIEAGKGSFPGHSTPIPQLYCCGDS 472

Query: 421 TFPGIGVPAVAASGAIVANSLVSTSQHSQLLDAIGI 456
           TFPGIGVPAVAASGAIVANSLVSTSQHSQLLDAIGI
Sbjct: 473 TFPGIGVPAVAASGAIVANSLVSTSQHSQLLDAIGI 506

BLAST of CmoCh16G008330.1 vs. NCBI nr
Match: XP_022929364.1 (prolycopene isomerase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 831.2 bits (2146), Expect = 4.2e-237
Identity = 418/442 (94.57%), Postives = 421/442 (95.25%), Query Frame = 0

Query: 16  WSTYLKESS-CHALAPQSFSRWISDNELDLETYASPNAVQEWQKLLETILPLSAAAMALP 75
           W  Y+ E      + P  F +       DLETYASPNAVQEWQKLLETILPLSAAAMALP
Sbjct: 146 WMVYIPEGEFLSRIGPTEFFK-------DLETYASPNAVQEWQKLLETILPLSAAAMALP 205

Query: 76  PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 135
           PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN
Sbjct: 206 PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 265

Query: 136 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 195
           WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR
Sbjct: 266 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 325

Query: 196 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 255
           ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV
Sbjct: 326 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 385

Query: 256 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 315
           NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP
Sbjct: 386 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 445

Query: 316 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVERALGPGFKREKCEVK 375
           PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE MWRAVERALGPGFKREKCEVK
Sbjct: 446 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEVMWRAVERALGPGFKREKCEVK 505

Query: 376 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 435
           LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG
Sbjct: 506 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 565

Query: 436 AIVANSLVSTSQHSQLLDAIGI 456
           AIVANSLVSTSQHSQLLDAIGI
Sbjct: 566 AIVANSLVSTSQHSQLLDAIGI 580

BLAST of CmoCh16G008330.1 vs. NCBI nr
Match: KAG6577364.1 (Prolycopene isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015453.1 Prolycopene isomerase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 829.3 bits (2141), Expect = 1.6e-236
Identity = 417/442 (94.34%), Postives = 421/442 (95.25%), Query Frame = 0

Query: 16  WSTYLKESS-CHALAPQSFSRWISDNELDLETYASPNAVQEWQKLLETILPLSAAAMALP 75
           W  Y+ E      + P  F +       DLETYASPNAVQEWQKLLETILPLSAAAMALP
Sbjct: 146 WMVYIPEGEFLSRIGPTEFFK-------DLETYASPNAVQEWQKLLETILPLSAAAMALP 205

Query: 76  PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 135
           PLSIRG+WGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN
Sbjct: 206 PLSIRGDWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 265

Query: 136 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 195
           WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR
Sbjct: 266 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 325

Query: 196 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 255
           ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV
Sbjct: 326 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 385

Query: 256 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 315
           NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP
Sbjct: 386 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 445

Query: 316 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVERALGPGFKREKCEVK 375
           PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE MWRAVERALGPGFKREKCEVK
Sbjct: 446 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEVMWRAVERALGPGFKREKCEVK 505

Query: 376 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 435
           LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG
Sbjct: 506 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 565

Query: 436 AIVANSLVSTSQHSQLLDAIGI 456
           AIVANSLVSTSQHSQLLDAIGI
Sbjct: 566 AIVANSLVSTSQHSQLLDAIGI 580

BLAST of CmoCh16G008330.1 vs. NCBI nr
Match: XP_023553449.1 (prolycopene isomerase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 824.7 bits (2129), Expect = 3.9e-235
Identity = 414/442 (93.67%), Postives = 419/442 (94.80%), Query Frame = 0

Query: 16  WSTYLKESS-CHALAPQSFSRWISDNELDLETYASPNAVQEWQKLLETILPLSAAAMALP 75
           W  Y+ E      + P  F +       DLE YASPNAVQEWQKLLETILPLSAAAMALP
Sbjct: 146 WMVYIPEGEFLSRIGPTEFFK-------DLEKYASPNAVQEWQKLLETILPLSAAAMALP 205

Query: 76  PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 135
           PLSIRG+WGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN
Sbjct: 206 PLSIRGDWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 265

Query: 136 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 195
           WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR
Sbjct: 266 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 325

Query: 196 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 255
           ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDT+ALLPESVVPKSYRDGV
Sbjct: 326 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTMALLPESVVPKSYRDGV 385

Query: 256 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 315
           NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP
Sbjct: 386 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 445

Query: 316 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVERALGPGFKREKCEVK 375
           PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE MWRAVERALGPGFKREKCEVK
Sbjct: 446 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEVMWRAVERALGPGFKREKCEVK 505

Query: 376 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 435
           LVGSPLTHQRFLRRNRGTYGPAIEAGK TFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG
Sbjct: 506 LVGSPLTHQRFLRRNRGTYGPAIEAGKGTFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 565

Query: 436 AIVANSLVSTSQHSQLLDAIGI 456
           AIVANSLVSTSQHSQLLDAIGI
Sbjct: 566 AIVANSLVSTSQHSQLLDAIGI 580

BLAST of CmoCh16G008330.1 vs. NCBI nr
Match: XP_022984389.1 (prolycopene isomerase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 823.5 bits (2126), Expect = 8.7e-235
Identity = 414/442 (93.67%), Postives = 419/442 (94.80%), Query Frame = 0

Query: 16  WSTYLKESS-CHALAPQSFSRWISDNELDLETYASPNAVQEWQKLLETILPLSAAAMALP 75
           W  Y+ E      + P  F +       DLETYASPNAVQEWQKLLETILPLSAAAMALP
Sbjct: 146 WMVYIPEGEFLSRIGPTEFFK-------DLETYASPNAVQEWQKLLETILPLSAAAMALP 205

Query: 76  PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 135
           PLSIRG+WGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN
Sbjct: 206 PLSIRGDWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 265

Query: 136 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 195
           WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR
Sbjct: 266 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 325

Query: 196 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 255
           ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV
Sbjct: 326 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 385

Query: 256 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 315
           NAMPQCESFMHLHLGFDA GIREDLGIHHIVVNDW+RGVDADQNVVLISVPSVLSPDLAP
Sbjct: 386 NAMPQCESFMHLHLGFDAVGIREDLGIHHIVVNDWERGVDADQNVVLISVPSVLSPDLAP 445

Query: 316 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVERALGPGFKREKCEVK 375
           PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE MWRAVERALGPGFKREKCEVK
Sbjct: 446 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEVMWRAVERALGPGFKREKCEVK 505

Query: 376 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 435
           LVGSPLTHQRFLRRNRGTYGPAIEAGK TFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG
Sbjct: 506 LVGSPLTHQRFLRRNRGTYGPAIEAGKGTFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 565

Query: 436 AIVANSLVSTSQHSQLLDAIGI 456
           AIVANSLVSTSQHSQLLDAIGI
Sbjct: 566 AIVANSLVSTSQHSQLLDAIGI 580

BLAST of CmoCh16G008330.1 vs. TAIR 10
Match: AT1G57770.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 714.9 bits (1844), Expect = 4.1e-206
Identity = 354/442 (80.09%), Postives = 390/442 (88.24%), Query Frame = 0

Query: 16  WSTYLKESS-CHALAPQSFSRWISDNELDLETYASPNAVQEWQKLLETILPLSAAAMALP 75
           W  YL E      + P  F +       DLE YA P+AVQEW+KLL  ILPLS+AAMALP
Sbjct: 140 WMVYLPEGDFLSRIGPTDFFK-------DLEKYAGPSAVQEWEKLLGAILPLSSAAMALP 199

Query: 76  PLSIRGNWGVLSTAAARYAPSLLKSFIQMGPQGILGSTKLLRPFSEIIDSLELNDPFIRN 135
           PLSIRG+ GVLSTAAARYAPSLLKSFI+MGP+G LG+TKLLRPFSEI+DSLEL DPFIRN
Sbjct: 200 PLSIRGDLGVLSTAAARYAPSLLKSFIKMGPKGALGATKLLRPFSEIVDSLELKDPFIRN 259

Query: 136 WLDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNALVQGLQKFGGR 195
           W+DLLAFLLAGVK++GILSAEMIYMFAEWYKPG  LEYP+ G+GAVV ALV+GL+KFGGR
Sbjct: 260 WIDLLAFLLAGVKSDGILSAEMIYMFAEWYKPGCTLEYPIDGTGAVVEALVRGLEKFGGR 319

Query: 196 ISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLALLPESVVPKSYRDGV 255
           +SL SHV+NI++EN +AVGVKL++GQF+RA+KAVVSNASMWDTL LLP   +P+SY  GV
Sbjct: 320 LSLKSHVENIVIENGKAVGVKLRNGQFVRARKAVVSNASMWDTLKLLPPGALPESYVKGV 379

Query: 256 NAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAP 315
           N  PQCESFMHLHLGFDAEGI +DL IHHIVVNDWDRGVDADQNVVLISVPSVLSP+LAP
Sbjct: 380 NTTPQCESFMHLHLGFDAEGIADDLEIHHIVVNDWDRGVDADQNVVLISVPSVLSPNLAP 439

Query: 316 PGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSE-MWRAVERALGPGFKREKCEVK 375
           PGKHVLHAY PGTEP+ LWEGLDRRS+EYKNLK+QRSE MWRAVERALG GFKREKCEV 
Sbjct: 440 PGKHVLHAYCPGTEPFGLWEGLDRRSAEYKNLKSQRSEVMWRAVERALGLGFKREKCEVS 499

Query: 376 LVGSPLTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGVPAVAASG 435
           LVG+PLTHQRFLRRNRGTYGPAIEAGK TFPGHSTPIPQL CCGDSTFPGIGVPAVAASG
Sbjct: 500 LVGTPLTHQRFLRRNRGTYGPAIEAGKGTFPGHSTPIPQLLCCGDSTFPGIGVPAVAASG 559

Query: 436 AIVANSLVSTSQHSQLLDAIGI 456
           AIVANSLV  S+HSQLLDAIG+
Sbjct: 560 AIVANSLVPVSKHSQLLDAIGL 574

BLAST of CmoCh16G008330.1 vs. TAIR 10
Match: AT1G06820.1 (carotenoid isomerase )

HSP 1 Score: 175.3 bits (443), Expect = 1.2e-43
Identity = 109/334 (32.63%), Postives = 179/334 (53.59%), Query Frame = 0

Query: 127 LNDPFIRNWLDLLAFLLAGVKT--NGILSAEMIYMFAEWYKPGSCLEYPLHGSGAVVNAL 186
           + DP + +++D   F+++ V      +++A M+     +      + YP+ G G +  +L
Sbjct: 257 IKDPQLLSFIDAECFIVSTVNALQTPMINASMVLCDRHY----GGINYPVGGVGGIAKSL 316

Query: 187 VQGLQKFGGRISLGSHVQNIIVENDRAVGVKLKSGQFIRAKKAVVSNASMWDTLA-LLPE 246
            +GL   G  I   ++V++II+++ +AVGV+L  G+   A K ++SNA+ WDT   LL  
Sbjct: 317 AEGLVDQGSEIQYKANVKSIILDHGKAVGVRLADGREFFA-KTIISNATRWDTFGKLLKG 376

Query: 247 SVVPKSYRDGVNAMPQCESFMHLHLGFDAEGIREDLGIHHIVVNDWDRGVDADQNVVLIS 306
             +PK   +      +  SF+ +H+G  AE +  D   HH V+ D  + ++     + +S
Sbjct: 377 EKLPKEEENFQKVYVKAPSFLSIHMGVKAEVLPPDTDCHHFVLEDDWKNLEEPYGSIFLS 436

Query: 307 VPSVLSPDLAPPGKHVLHAYTPGTEPYTLWEGLDRRSSEYKNLKAQRSEMWRAVERALGP 366
           +P++L   LAP G+H+LH +T  T     WEGL  +  E K  +   + + + +E+ L P
Sbjct: 437 IPTILDSSLAPDGRHILHIFT--TSSIEDWEGLPPKEYEAKK-EDVAARIIQRLEKKLFP 496

Query: 367 GFKREKCEVKLVGSPLTHQRFLRRNRGTYGP---AIEAGKATFPGHSTPIPQLYCCGDST 426
           G        K VG+P TH+RFL R++GTYGP       G    P ++T I  LYC GDS 
Sbjct: 497 GLS-SSITFKEVGTPRTHRRFLARDKGTYGPMPRGTPKGLLGMPFNTTAIDGLYCVGDSC 556

Query: 427 FPGIGVPAVAASGAIVANSL---VSTSQHSQLLD 452
           FPG GV AVA SG + A+ +   +   + S++LD
Sbjct: 557 FPGQGVIAVAFSGVMCAHRVAADIGLEKKSRVLD 581

BLAST of CmoCh16G008330.1 vs. TAIR 10
Match: AT5G49555.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 69.3 bits (168), Expect = 8.9e-12
Identity = 80/348 (22.99%), Postives = 140/348 (40.23%), Query Frame = 0

Query: 114 LLRPFSEIIDSLELNDPFIRNW-LDLLAFLLAGVKTNGILSAEMIYMFAEWYKPGSCLEY 173
           LL P S+++++   +D    +   D +    A V T G     + ++  E         Y
Sbjct: 199 LLAPASKVLNNWFESDVLKASLATDAVIGSTASVHTPGSGYVLLHHVMGETDGEKGIWSY 258

Query: 174 PLHGSGAVVNALVQGLQKFGGRISLGSHVQNIIVENDRAV-GVKLKSGQFIRAKKAVVSN 233
              G G+V  A+    ++ G  I   + V  I+ E+   V GV L  G  + +  A++SN
Sbjct: 259 VEGGMGSVSMAIANAAKEAGAEIFTNAEVSEILTEDSSIVKGVLLADGTRVES-SAILSN 318

Query: 234 ASMWDT-LALLPESVVPKSYRD----------------GVNAMPQCESFMHLHLGFDAEG 293
           A+ + T + L+P +V+P+++                   V+ +PQ +     H G   E 
Sbjct: 319 ATPYRTYVELVPTNVLPENFVSAIKNSDYSSATTKINLAVDKLPQFQCCNTNHSGPGPEH 378

Query: 294 I------REDLGIHHIVVNDWDRGVDADQNVVLISVPSVLSPDLAPPGKHVLH---AYTP 353
                   E +   H   +D + G+ + + V+ +++PS L   ++PPGKHV++    YTP
Sbjct: 379 FGTIHIGAESMDEVHSACHDSENGLPSRRPVIEMTIPSTLDNTISPPGKHVINLFIQYTP 438

Query: 354 GTEPYTLWEGLDRRSSEYKNLKAQRSEMWRAVERALGPGFKREKCEVKLVGSP------- 413
                  WE        Y+   AQR   ++ ++    PGF        ++  P       
Sbjct: 439 YKPSDGSWE-----DPTYREAFAQR--CFKLIDE-YAPGFSSSIISYDMLTPPDLEREIG 498

Query: 414 LTHQRFLRRNRGTYGPAIEAGKATFPGHSTPIPQLYCCGDSTFPGIGV 427
           LT         G     +      +  + +P+  LY CG    PG GV
Sbjct: 499 LTGGNIFHGAMGLDSLFLMRPVKGWSNYRSPLKGLYLCGSGAHPGGGV 537

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2VEX93.9e-4433.73Prolycopene isomerase, chloroplastic OS=Daucus carota OX=4039 GN=CRTISO PE=2 SV=... [more]
Q52QW31.2e-4234.06Prolycopene isomerase 1, chloroplastic OS=Oncidium hybrid cultivar OX=141207 GN=... [more]
Q52QW21.2e-4234.06Prolycopene isomerase 2, chloroplastic OS=Oncidium hybrid cultivar OX=141207 GN=... [more]
Q8S4R41.2e-4233.73Prolycopene isomerase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=CRTISO P... [more]
Q9M9Y81.6e-4232.63Prolycopene isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRTISO P... [more]
Match NameE-valueIdentityDescription
A0A1S4DZ577.2e-24393.20prolycopene isomerase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1ENI92.0e-23794.57prolycopene isomerase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1114359... [more]
A0A6J1JAD04.2e-23593.67prolycopene isomerase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111482703... [more]
A0A0A0L3B19.1e-23091.18Amino_oxidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G1100... [more]
A0A6J1C5F21.5e-22991.40prolycopene isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111008... [more]
Match NameE-valueIdentityDescription
XP_016901253.11.5e-24293.20PREDICTED: prolycopene isomerase, chloroplastic isoform X2 [Cucumis melo][more]
XP_022929364.14.2e-23794.57prolycopene isomerase, chloroplastic [Cucurbita moschata][more]
KAG6577364.11.6e-23694.34Prolycopene isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_023553449.13.9e-23593.67prolycopene isomerase, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022984389.18.7e-23593.67prolycopene isomerase, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G57770.14.1e-20680.09FAD/NAD(P)-binding oxidoreductase family protein [more]
AT1G06820.11.2e-4332.63carotenoid isomerase [more]
AT5G49555.18.9e-1222.99FAD/NAD(P)-binding oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.660.50coord: 128..388
e-value: 3.6E-21
score: 77.6
NoneNo IPR availablePANTHERPTHR46313:SF1FAD/NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEINcoord: 37..455
NoneNo IPR availablePANTHERPTHR46313FAMILY NOT NAMEDcoord: 37..455
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 171..419
e-value: 3.6E-21
score: 77.6
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 114..441

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh16G008330CmoCh16G008330gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh16G008330.1:exon:8049CmoCh16G008330.1:exon:8049exon
CmoCh16G008330.1:exon:8050CmoCh16G008330.1:exon:8050exon
CmoCh16G008330.1:exon:8051CmoCh16G008330.1:exon:8051exon
CmoCh16G008330.1:exon:8052CmoCh16G008330.1:exon:8052exon
CmoCh16G008330.1:exon:8053CmoCh16G008330.1:exon:8053exon
CmoCh16G008330.1:exon:8054CmoCh16G008330.1:exon:8054exon
CmoCh16G008330.1:exon:8055CmoCh16G008330.1:exon:8055exon
CmoCh16G008330.1:exon:8056CmoCh16G008330.1:exon:8056exon
CmoCh16G008330.1:exon:8057CmoCh16G008330.1:exon:8057exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh16G008330.1:five_prime_utrCmoCh16G008330.1:five_prime_utrfive_prime_UTR
CmoCh16G008330.1:five_prime_utrCmoCh16G008330.1:five_prime_utr_2five_prime_UTR
CmoCh16G008330.1:five_prime_utrCmoCh16G008330.1:five_prime_utr_3five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh16G008330.1:cdsCmoCh16G008330.1:cdsCDS
CmoCh16G008330.1:cdsCmoCh16G008330.1:cds_2CDS
CmoCh16G008330.1:cdsCmoCh16G008330.1:cds_3CDS
CmoCh16G008330.1:cdsCmoCh16G008330.1:cds_4CDS
CmoCh16G008330.1:cdsCmoCh16G008330.1:cds_5CDS
CmoCh16G008330.1:cdsCmoCh16G008330.1:cds_6CDS
CmoCh16G008330.1:cdsCmoCh16G008330.1:cds_7CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh16G008330.1:three_prime_utrCmoCh16G008330.1:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh16G008330.1CmoCh16G008330.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016853 isomerase activity
molecular_function GO:0016491 oxidoreductase activity