CmoCh16G003580.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G003580.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	alpha-L-fucosidase 2-like
Location	Cmo_Chr16: 1653937 .. 1656203 (-)
Sequence length	1335
RNA-Seq Expression	CmoCh16G003580.1
Synteny	CmoCh16G003580.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTATTGGCCCTCCCTTCCATGCAACCTCAAGGAGTGCCAGGAACCATTGTTTGATTTCACATCCTTCTTGTCTGTCAATGGACGCAAGACAGCAAAAGTAAGCATCTTTACCTCTGATAGCATCCAAGCCATCACAAGATTTCCATCATAGGGAAGAAACATTCTCAACCTTGTCTGTTTCATTGACCTCACCTGTTCTTGAACATTATAGGTGAACTATGAAGCAAATGGTTGGGTAGCTCATCAAGTGTCAGACATCTGGGCAAAAAGCTCTCCGGATCAAGGGCAGGCCGTTTGGGCTCTATGGCCAATGGGTGGAGCTTGGCTCTGTACCCATCTGTGGGAGCATTACACATACACAATGGACAAGGTAAGTTCTTGCTTGTCCTTTGTACTGCAAATTCATAAGCTGTCACCTCTAAATGGAACTTTTGGTGCAGAATTTCTTAAGAAACAAGGCATACCCTTTGATGGAAGGATGCACATCGTTTCTGCTGGATTGGCTAATAGATGGCAAGAATGGATATCTAGAAACAAACCCATCGACATCCCCAGAGCACATGTTTATAGCTCCTGATGGTAAGCCTGCTAGTGTGAGCTACTCAACAACAATGGATATTGCTATCATAAAGGAGGTTTTCTCAGCCATCATAGCTGCTGCAGAGGTGAAGCTTGAACTGAAACCTTTTTTTACTTCATGTACTGCACGGATTTGTTTATGTTTTTCTTTACAGATTCTAGGAAGAAGCAAGGATGCTATTATTGACAAGGTTCGGAAGGCTGAAGCTAGGCTATTGCCTTATAAGATAGCTAGAGATGGTTCTCTTATGGAATGGGTATGTAAGTATCTCGTCTCTTAACCAAGAACATTGAAGAACTAACTATCTACACCGCCCTAACTCGACTAACCCGACTAACTTGACGACGTTTCTTGGTTAGGCTATTGATTTTGAGGACCAAGAAGTGCATCATCGACATGTATCACACCTGTTTGGGCTGTATCCAGGACACACCATATCTGTTGAGAGAACTCCCAACATTTCGGAAGCTGCAAATAATACTCTTCATAAGAGAGGTTTCTATTTGTCAACCAAATTCTTTTCTTCTACTTTAGGCTTTATAGACATAGGGTGCTCTAGATTATCTCGTGCCAATGAAGTGAGATCCCACATCGGTTAGAGAAGGAAACGAAGCATTCTTTATAAGGGTGAAGAAACCTCTCCCTAGCGGACGCGTTTTAAAACCTTGAGAAGCTCGGGAGGGAAAGCCCAAAGGGACAATATCTGCTAGTGGTGGGCTTGGGTTGTTGCAAATGGTATTAGAGCCAAACACCAAGCGGTGTGCCAGTGAGGACGTTGGGGCTCGAAGGAGAGTGGATTATGAGATCCCACATCGGTTGGGAGTAGAACGAAGCATTCTTTATAAGGATGTGGAAACCTCTCCCTAGCAAACGCGTTTTAAAAACCTTGAGGGAAAGTCTGAAATGGAAAGCTAAAAAAAGACAATATCTATTAGCTTGATCACTGCACAAAACAACTGTGGTTCATGTGAAACAGGAGAGGAAGGTCCAGGATGGTCGACTGTGTGGAAGATCGCGCTATGGGCTCGTCTTTGGAACGGTGAGCACGCATACCGAATGGTGAAGCATTTGTTCGAGCTCGTGGATCCAGAGCATGAAAGTGATTATGAAGGTGGCCTGTACAGCAATTTGTTCACTGCTCATCCCCCTTTCCAAATAGATGCCAACTTTGGGTAAGCTCAAATGAAACACTATCCTTTCTAGCTACATAGTTTACCTGATCATTGCCTGCTCCTTCTGTTGGGCTATCCAGATTCTCAGCTGCCATTGCTGAAATGCTGGTCCAGAGCACTGTCAAGGACCTGTACTTGCTTCCTGCTCTTCCCGGTGATGTCTGGTCCGACGGTTGCGTTAAAGGCTTGAAGGCGCGGGGTGGCTTGACGGTAAACATCTGCTGGAAGGGAGGCGATCTCGACGAGGTCGGTGTTTGGTCGTCGGAACAAATTTCGGTTACAACATTACACTACAGAGGGAGTAAAGTAGCAGCAAATTTAACATCTGGTAAACTCTACACTTTCAATAAGCTGTTGAAATGTGTTGGAACATGCTCTCTTCTACCAAACAGCAATGGGTTTCCAAATCCTTGAAGCACATGTTCTTTGATGATTCTTTCTGTAACATGAAAGGTTTCCAAATCCTTAAATATAATTTCTAAGTTTGTTCGGCCACAAACACTGCCTGCA

mRNA sequence

ATGAACTATTGGCCCTCCCTTCCATGCAACCTCAAGGAGTGCCAGGAACCATTGTTTGATTTCACATCCTTCTTGTCTGTCAATGGACGCAAGACAGCAAAAGTGAACTATGAAGCAAATGGTTGGGTAGCTCATCAAGTGTCAGACATCTGGGCAAAAAGCTCTCCGGATCAAGGGCAGGCCGTTTGGGCTCTATGGCCAATGGGTGGAGCTTGGCTCTGTACCCATCTGTGGGAGCATTACACATACACAATGGACAAGAATTTCTTAAGAAACAAGGCATACCCTTTGATGGAAGGATGCACATCGTTTCTGCTGGATTGGCTAATAGATGGCAAGAATGGATATCTAGAAACAAACCCATCGACATCCCCAGAGCACATGTTTATAGCTCCTGATGGTAAGCCTGCTAGTGTGAGCTACTCAACAACAATGGATATTGCTATCATAAAGGAGGTTTTCTCAGCCATCATAGCTGCTGCAGAGATTCTAGGAAGAAGCAAGGATGCTATTATTGACAAGGTTCGGAAGGCTGAAGCTAGGCTATTGCCTTATAAGATAGCTAGAGATGGTTCTCTTATGGAATGGGCTATTGATTTTGAGGACCAAGAAGTGCATCATCGACATGTATCACACCTGTTTGGGCTGTATCCAGGACACACCATATCTGTTGAGAGAACTCCCAACATTTCGGAAGCTGCAAATAATACTCTTCATAAGAGAGGATGGTCGACTGTGTGGAAGATCGCGCTATGGGCTCGTCTTTGGAACGGTGAGCACGCATACCGAATGGTGAAGCATTTGTTCGAGCTCGTGGATCCAGAGCATGAAAGTGATTATGAAGGTGGCCTGTACAGCAATTTGTTCACTGCTCATCCCCCTTTCCAAATAGATGCCAACTTTGGATTCTCAGCTGCCATTGCTGAAATGCTGGTCCAGAGCACTGTCAAGGACCTGTACTTGCTTCCTGCTCTTCCCGGTGATGTCTGGTCCGACGGTTGCGTTAAAGGCTTGAAGGCGCGGGGTGGCTTGACGGTAAACATCTGCTGGAAGGGAGGCGATCTCGACGAGGTCGGTGTTTGGTCGTCGGAACAAATTTCGGTTACAACATTACACTACAGAGGGAGTAAAGTAGCAGCAAATTTAACATCTGGTAAACTCTACACTTTCAATAAGCTGTTGAAATGTGTTGGAACATGCTCTCTTCTACCAAACAGCAATGGGTTTCCAAATCCTTGAAGCACATGTTCTTTGATGATTCTTTCTGTAACATGAAAGGTTTCCAAATCCTTAAATATAATTTCTAAGTTTGTTCGGCCACAAACACTGCCTGCA

Coding sequence (CDS)

ATGAACTATTGGCCCTCCCTTCCATGCAACCTCAAGGAGTGCCAGGAACCATTGTTTGATTTCACATCCTTCTTGTCTGTCAATGGACGCAAGACAGCAAAAGTGAACTATGAAGCAAATGGTTGGGTAGCTCATCAAGTGTCAGACATCTGGGCAAAAAGCTCTCCGGATCAAGGGCAGGCCGTTTGGGCTCTATGGCCAATGGGTGGAGCTTGGCTCTGTACCCATCTGTGGGAGCATTACACATACACAATGGACAAGAATTTCTTAAGAAACAAGGCATACCCTTTGATGGAAGGATGCACATCGTTTCTGCTGGATTGGCTAATAGATGGCAAGAATGGATATCTAGAAACAAACCCATCGACATCCCCAGAGCACATGTTTATAGCTCCTGATGGTAAGCCTGCTAGTGTGAGCTACTCAACAACAATGGATATTGCTATCATAAAGGAGGTTTTCTCAGCCATCATAGCTGCTGCAGAGATTCTAGGAAGAAGCAAGGATGCTATTATTGACAAGGTTCGGAAGGCTGAAGCTAGGCTATTGCCTTATAAGATAGCTAGAGATGGTTCTCTTATGGAATGGGCTATTGATTTTGAGGACCAAGAAGTGCATCATCGACATGTATCACACCTGTTTGGGCTGTATCCAGGACACACCATATCTGTTGAGAGAACTCCCAACATTTCGGAAGCTGCAAATAATACTCTTCATAAGAGAGGATGGTCGACTGTGTGGAAGATCGCGCTATGGGCTCGTCTTTGGAACGGTGAGCACGCATACCGAATGGTGAAGCATTTGTTCGAGCTCGTGGATCCAGAGCATGAAAGTGATTATGAAGGTGGCCTGTACAGCAATTTGTTCACTGCTCATCCCCCTTTCCAAATAGATGCCAACTTTGGATTCTCAGCTGCCATTGCTGAAATGCTGGTCCAGAGCACTGTCAAGGACCTGTACTTGCTTCCTGCTCTTCCCGGTGATGTCTGGTCCGACGGTTGCGTTAAAGGCTTGAAGGCGCGGGGTGGCTTGACGGTAAACATCTGCTGGAAGGGAGGCGATCTCGACGAGGTCGGTGTTTGGTCGTCGGAACAAATTTCGGTTACAACATTACACTACAGAGGGAGTAAAGTAGCAGCAAATTTAACATCTGGTAAACTCTACACTTTCAATAAGCTGTTGAAATGTGTTGGAACATGCTCTCTTCTACCAAACAGCAATGGGTTTCCAAATCCTTGA

Protein sequence

MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQAVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETNPSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEARLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHKRGWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPFQIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDLDEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP

Homology

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BLAST of CmoCh16G003580.1 vs. ExPASy Swiss-Prot
Match: Q8L7W8 (Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 3.3e-175
Identity = 286/407 (70.27%), Postives = 341/407 (83.78%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYW SLP N++ECQEPLFD+ S L++NGRKTA+VNY A+GWVAHQVSDIWAK+SPD+G+
Sbjct: 438 MNYWHSLPGNIRECQEPLFDYMSALAINGRKTAQVNYGASGWVAHQVSDIWAKTSPDRGE 497

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTH WEHYTYTMDK FL+ K YPL+EGCTSFLLDWLI GK+G+L+TN
Sbjct: 498 AVWALWPMGGAWLCTHAWEHYTYTMDKEFLKKKGYPLLEGCTSFLLDWLIKGKDGFLQTN 557

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMF AP GKPASVSYS+TMDIAIIKEVF+ I++A+EILG++ D +I KV  A+A
Sbjct: 558 PSTSPEHMFTAPIGKPASVSYSSTMDIAIIKEVFADIVSASEILGKTNDTLIGKVIAAQA 617

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           +L P +I++DGS+ EWA DFED EVHHRHVSHLFGL+PGHTI+VE++P +++A   TL K
Sbjct: 618 KLPPTRISKDGSIREWAEDFEDPEVHHRHVSHLFGLFPGHTITVEKSPELAKAVEATLKK 677

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWST WK ALWARL N EHAYRMV H+F+LVDP +E +YEGGLYSN+FTAHPPF
Sbjct: 678 RGEEGPGWSTTWKAALWARLHNSEHAYRMVTHIFDLVDPLNERNYEGGLYSNMFTAHPPF 737

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGF+AA+AEMLVQST KDLYLLPALP D W +G V GL+ARGG+TV+I W  G+L
Sbjct: 738 QIDANFGFAAAVAEMLVQSTTKDLYLLPALPADKWPNGIVNGLRARGGVTVSIKWMEGNL 797

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSL 403
            E G+W SEQI  T + YRG   AA L  GK++TF+K L+C+ T  L
Sbjct: 798 VEFGLW-SEQIVSTRIVYRGISAAAELLPGKVFTFDKDLRCIRTDKL 843

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BLAST of CmoCh16G003580.1 vs. ExPASy Swiss-Prot
Match: Q5AU81 (Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=afcA PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.9e-46
Identity = 125/380 (32.89%), Postives = 184/380 (48.42%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSV-NGRKTAKVNYEANGWVAHQVSDIWAKSSPDQG 60
           MNYW +    L E Q  L+++ +   V  G +TA++ Y A+GWV H   +I+  ++  + 
Sbjct: 414 MNYWLADQTGLGETQHALWNYMADTWVPRGTETARLLYNASGWVVHNEINIFGFTAMKE- 473

Query: 61  QAVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGK---NGY 120
            A WA +P   AW+  H+W+++ YT D  +L ++ Y L++G  SF L  L + K   +G 
Sbjct: 474 DAGWANYPAAAAWMMQHVWDNFDYTHDTAWLVSQGYALLKGIASFWLSSLQEDKFFNDGS 533

Query: 121 LETNPSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVR 180
           L  NP  SPE             ++  T    +I +VF  ++AA E +  S    +D V 
Sbjct: 534 LVVNPCNSPE---------TGPTTFGCTHYQQLIHQVFETVLAAQEYIHESDTKFVDSVA 593

Query: 181 KAEARL-LPYKIARDGSLMEWAIDFE---DQEVHHRHVSHLFGLYPGHTISV----ERTP 240
            A  RL     ++  G L EW +      D    HRH+SHL G YPG++IS      R  
Sbjct: 594 SALERLDTGLHLSSWGGLKEWKLPDSYGYDNMSTHRHLSHLAGWYPGYSISSFAHGYRNK 653

Query: 241 NISEAANNTLHKR----------GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHES 300
            I +A   TL  R          GW+ VW+ A WARL +   AY  +++         + 
Sbjct: 654 TIQDAVKETLTARGMGNAADANAGWAKVWRAACWARLNDSSMAYDELRYAI-------DE 713

Query: 301 DYEGGLYSNLFTAHPPFQIDANFGFSAAIAEMLV--------QSTVKDLYLLPALPGDVW 351
           ++ G   S  + A PPFQIDANFGF+ A+  MLV            + + L PA+P   W
Sbjct: 714 NFVGNGLSMYWGASPPFQIDANFGFAGAVLSMLVVDLPTPRSDPGQRTVVLGPAIP-SAW 773

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BLAST of CmoCh16G003580.1 vs. ExPASy Swiss-Prot
Match: A2R797 (Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.2e-41
Identity = 116/378 (30.69%), Postives = 182/378 (48.15%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSV-NGRKTAKVNY-EANGWVAHQVSDIWAKSSPDQ 60
           MN+W      L E  EPL+ + +   +  G +TA++ Y  + GWV H   + +  ++  +
Sbjct: 394 MNHWAVDQTGLGELTEPLWTYMAETWMPRGAETAELLYGTSEGWVTHDEMNTFGHTAM-K 453

Query: 61  GQAVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDG---KNG 120
             A WA +P   AW+  H+W+H+ Y+ D  + R   YP+++G   F L  L+     K+G
Sbjct: 454 DVAQWADYPATNAWMSHHVWDHFDYSQDSAWYRETGYPILKGAAQFWLSQLVKDEYFKDG 513

Query: 121 YLETNPSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKV 180
            L  NP  SPEH    P   P   ++  T    +I E+F  ++      G    +  + +
Sbjct: 514 TLVVNPCNSPEH---GPTLTPQ--TFGCTHYQQLIWELFDHVLQGWTASGDDDTSFKNAI 573

Query: 181 RKAEARLLP-YKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPN--ISE 240
               + L P   I   G + EW +D + +   HRH+S+L+G YPG+ IS     N  I++
Sbjct: 574 TSKFSTLDPGIHIGSWGQIQEWKLDIDVKNDTHRHLSNLYGWYPGYIISSVHGSNKTITD 633

Query: 241 AANNTLHKR---------GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGG 300
           A   TL+ R         GW+ VW+ A WA L   + AY       EL     ++  E G
Sbjct: 634 AVETTLYSRGTGVEDSNTGWAKVWRSACWALLNVTDEAYS------ELSLAIQDNFAENG 693

Query: 301 LYSNLFTAHPPFQIDANFGFSAAIAEMLVQST-----------VKDLYLLPALPGDVWSD 351
              ++++  PPFQIDANFG   A+ +ML++ +            +D+ L PA+P   W  
Sbjct: 694 F--DMYSGSPPFQIDANFGLVGAMVQMLIRDSDRSSADASAGKTQDVLLGPAIPA-AWGG 753

[Close]

BLAST of CmoCh16G003580.1 vs. ExPASy Swiss-Prot
Match: Q2USL3 (Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=afcA PE=3 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 1.8e-24
Identity = 87/268 (32.46%), Postives = 120/268 (44.78%), Query Frame = 0


Query: 110 IDGKNGYLETNPSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKD 169
           I G  G L  NP TSPE             ++  T    +I +V+   I  AEI G +  
Sbjct: 441 IFGHTGSLVVNPCTSPEQ---------GPTTFGCTHWQQLIHQVYENAIQGAEIAGETDS 500

Query: 170 AIIDKVRKAEARL-LPYKIARDGSLMEWAI----DFEDQEVHHRHVSHLFGLYPGHTISV 229
            ++  ++    RL     I   G + EW +    D+E +   HRH+SHL G YPG ++S 
Sbjct: 501 TLLKDIKDQLPRLDKGLHIGTWGQIKEWKLPDSYDYEKEGNEHRHLSHLVGWYPGWSLSS 560

Query: 230 ----ERTPNISEAANNTLHKR--------GWSTVWKIALWARLWNGEHAYRMVKHLFELV 289
                    I  A N +L  R        GW  VW+ A WARL N E A+    +   L 
Sbjct: 561 YFNGYNNATIQSAVNTSLISRGVGLYTNAGWEKVWRSACWARLNNTEKAH----YELRLT 620

Query: 290 DPEHESDYEGGLYSNLFTAHPPFQIDANFGFSAAIAEMLV----------QSTVKDLYLL 349
             ++       LYS   T    FQIDANFG+  A+  MLV          +  V+ + L 
Sbjct: 621 IDQNIGQSGLSLYSGGDTPSGAFQIDANFGYLGAVLSMLVVDMPLDSTHSEDDVRTVVLG 680

Query: 350 PALPGDVWSDGCVKGLKARGGLTVNICW 351
           PA+P   W+ G VKGL+ RGG +V+  W
Sbjct: 681 PAIPA-AWAGGSVKGLRLRGGGSVDFSW 694

[Close]

BLAST of CmoCh16G003580.1 vs. ExPASy TrEMBL
Match: A0A6J1E566 (alpha-L-fucosidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430687 PE=4 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.0e-248
Identity = 412/417 (98.80%), Postives = 412/417 (98.80%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ
Sbjct: 414 MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 473

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN
Sbjct: 474 AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 533

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA
Sbjct: 534 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 593

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK
Sbjct: 594 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 653

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF
Sbjct: 654 RGEEGPGWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 713

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL
Sbjct: 714 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 773

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP 413
           DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP
Sbjct: 774 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP 830

[Close]

BLAST of CmoCh16G003580.1 vs. ExPASy TrEMBL
Match: A0A6J1JA05 (alpha-L-fucosidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482622 PE=4 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 6.7e-240
Identity = 399/417 (95.68%), Postives = 402/417 (96.40%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ
Sbjct: 414 MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 473

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN
Sbjct: 474 AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 533

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKD IIDKVRKA+A
Sbjct: 534 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDTIIDKVRKAQA 593

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTI+VE+TPNISEAANNTLHK
Sbjct: 594 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTITVEKTPNISEAANNTLHK 653

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWST WKIALWARL N E AYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF
Sbjct: 654 RGEEGPGWSTAWKIALWARLRNSEQAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 713

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGFSAAIAEMLVQSTVKDLYLLPALP DVWSDGCVKGLKARGGLTVNICWKGGDL
Sbjct: 714 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPCDVWSDGCVKGLKARGGLTVNICWKGGDL 773

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP 413
           DEVGVWSSEQISVTTLHYRGSKVA NLTSGKLYTFNK LKC  TCSLLPNSNGFPNP
Sbjct: 774 DEVGVWSSEQISVTTLHYRGSKVAVNLTSGKLYTFNKQLKCARTCSLLPNSNGFPNP 830

[Close]

BLAST of CmoCh16G003580.1 vs. ExPASy TrEMBL
Match: A0A6J1IV53 (alpha-L-fucosidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111478832 PE=4 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.7e-222
Identity = 364/407 (89.43%), Postives = 392/407 (96.31%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYWP+LPCNL+ECQEPLFDFTSFLSVNGRKTAKVNYEA+GWVAHQVSDIWAKSSPD+GQ
Sbjct: 424 MNYWPALPCNLRECQEPLFDFTSFLSVNGRKTAKVNYEASGWVAHQVSDIWAKSSPDRGQ 483

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTHLWEHYTYTMDKNFL+NKAYPLMEGCTSFLLDWLI+GK+GYLETN
Sbjct: 484 AVWALWPMGGAWLCTHLWEHYTYTMDKNFLKNKAYPLMEGCTSFLLDWLIEGKDGYLETN 543

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFS+I++AAEILG+SKDAII+KVR A++
Sbjct: 544 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSSIVSAAEILGKSKDAIINKVRMAQS 603

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           RLLPYKIARDGSLMEWA+DFED+EVHHRHVSHLFGLYPGHTI+VE+TPN+S+AA+NTLHK
Sbjct: 604 RLLPYKIARDGSLMEWALDFEDREVHHRHVSHLFGLYPGHTITVEKTPNVSKAADNTLHK 663

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWST WKIALWARL N EHAYRMVKHLF+LVDP+HESDYEGGLYSNLFTAHPPF
Sbjct: 664 RGEEGPGWSTAWKIALWARLQNSEHAYRMVKHLFDLVDPDHESDYEGGLYSNLFTAHPPF 723

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGFSAAIAEMLVQST+ DLYLLPALP DVW  GCVKGLKARGGLTVNICW GGDL
Sbjct: 724 QIDANFGFSAAIAEMLVQSTMNDLYLLPALPRDVWPYGCVKGLKARGGLTVNICWTGGDL 783

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSL 403
           +EVGVW+SEQISVTTLHYRG+KVAANLTSGK+YTFNKLLKCVGT SL
Sbjct: 784 NEVGVWTSEQISVTTLHYRGTKVAANLTSGKVYTFNKLLKCVGTYSL 830

[Close]

BLAST of CmoCh16G003580.1 vs. ExPASy TrEMBL
Match: A0A6J1FNV4 (alpha-L-fucosidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111447154 PE=4 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.1e-221
Identity = 360/407 (88.45%), Postives = 392/407 (96.31%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYWP+LPCNL+ECQEPLFDFT+FLSVNGRKTAKVNYEA+GWVAHQVSDIWAKSSPD+GQ
Sbjct: 424 MNYWPALPCNLRECQEPLFDFTNFLSVNGRKTAKVNYEASGWVAHQVSDIWAKSSPDRGQ 483

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTHLWEHYTYTMDKNFL+NKAYPLMEGCTSFLLDWLI+GK+GYLETN
Sbjct: 484 AVWALWPMGGAWLCTHLWEHYTYTMDKNFLKNKAYPLMEGCTSFLLDWLIEGKDGYLETN 543

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFS+I++AAEILG+SKDAII+KVR A++
Sbjct: 544 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSSIVSAAEILGKSKDAIINKVRMAQS 603

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           RLLPYKIARDGSLMEWA+DFED+EVHHRHVSHLFGLYPGHTI+VE+TPN+S+AA+NTLHK
Sbjct: 604 RLLPYKIARDGSLMEWALDFEDREVHHRHVSHLFGLYPGHTITVEKTPNVSKAADNTLHK 663

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWST WKIALWARL N EHAY+MVKHLF+LVDP+HESDYEGGLYSNLFTAHPPF
Sbjct: 664 RGEEGPGWSTAWKIALWARLQNSEHAYQMVKHLFDLVDPDHESDYEGGLYSNLFTAHPPF 723

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGFSAAIAEMLVQS++ DLYLLPALP DVW  GCVKGLKARGGLTVN+CW GGDL
Sbjct: 724 QIDANFGFSAAIAEMLVQSSMNDLYLLPALPRDVWPYGCVKGLKARGGLTVNVCWTGGDL 783

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSL 403
           +EVGVWSSEQIS+TTLHYRG+KVAANLTSGK+YTFNKLLKCVGT SL
Sbjct: 784 NEVGVWSSEQISLTTLHYRGTKVAANLTSGKVYTFNKLLKCVGTYSL 830

[Close]

BLAST of CmoCh16G003580.1 vs. ExPASy TrEMBL
Match: A0A5D3CPM6 (Alpha-L-fucosidase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G00640 PE=4 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.7e-219
Identity = 361/407 (88.70%), Postives = 385/407 (94.59%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEA+GWVAHQVSDIWAKSSPD+GQ
Sbjct: 408 MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEASGWVAHQVSDIWAKSSPDRGQ 467

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTHLWEHYTYTMDKNFL+NKAYPLMEGC SFLLDWLI+GK+GYLETN
Sbjct: 468 AVWALWPMGGAWLCTHLWEHYTYTMDKNFLKNKAYPLMEGCASFLLDWLINGKDGYLETN 527

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMFIAPDGKPASVSYSTTMDIAI KEVFS+II+AAEILG+SKD  IDKVRKA+A
Sbjct: 528 PSTSPEHMFIAPDGKPASVSYSTTMDIAITKEVFSSIISAAEILGKSKDTFIDKVRKAQA 587

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           RLLPYKIA+DGSLMEWA+DFED+EVHHRHVSHLFGLYPGHTI+V++TPNISEAANNTLHK
Sbjct: 588 RLLPYKIAKDGSLMEWALDFEDREVHHRHVSHLFGLYPGHTITVQKTPNISEAANNTLHK 647

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWST WKIALWARL N EHAYRMVKHLF+LVDP+HESDYEGGLYSNLFTAHPPF
Sbjct: 648 RGEEGPGWSTAWKIALWARLHNSEHAYRMVKHLFDLVDPDHESDYEGGLYSNLFTAHPPF 707

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGFSAAIAEMLVQST+ DLYLLPALP  VW  GCVKGLKARGGLTVN+CW GGDL
Sbjct: 708 QIDANFGFSAAIAEMLVQSTINDLYLLPALPRHVWPYGCVKGLKARGGLTVNMCWTGGDL 767

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSL 403
           DEVG+WSSEQIS+TTLHYR + VAANL+SGK+YTFNKLLKCV T SL
Sbjct: 768 DEVGLWSSEQISLTTLHYRETTVAANLSSGKVYTFNKLLKCVRTYSL 814

[Close]

BLAST of CmoCh16G003580.1 vs. NCBI nr
Match: XP_022922821.1 (alpha-L-fucosidase 2-like [Cucurbita moschata])

HSP 1 Score: 868.6 bits (2243), Expect = 2.1e-248
Identity = 412/417 (98.80%), Postives = 412/417 (98.80%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ
Sbjct: 414 MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 473

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN
Sbjct: 474 AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 533

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA
Sbjct: 534 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 593

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK
Sbjct: 594 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 653

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF
Sbjct: 654 RGEEGPGWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 713

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL
Sbjct: 714 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 773

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP 413
           DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP
Sbjct: 774 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP 830

[Close]

BLAST of CmoCh16G003580.1 vs. NCBI nr
Match: XP_023551736.1 (alpha-L-fucosidase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 856.7 bits (2212), Expect = 8.4e-245
Identity = 407/417 (97.60%), Postives = 408/417 (97.84%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ
Sbjct: 409 MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 468

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN
Sbjct: 469 AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 528

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKD IIDKVRKAEA
Sbjct: 529 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDTIIDKVRKAEA 588

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTI+VERTPNISEAANNTLHK
Sbjct: 589 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTITVERTPNISEAANNTLHK 648

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWST WKIALWARL NGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF
Sbjct: 649 RGEEGPGWSTAWKIALWARLRNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 708

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL
Sbjct: 709 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 768

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP 413
           DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTC LLPNSNGFPNP
Sbjct: 769 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCFLLPNSNGFPNP 825

[Close]

BLAST of CmoCh16G003580.1 vs. NCBI nr
Match: XP_022984253.1 (alpha-L-fucosidase 2-like [Cucurbita maxima])

HSP 1 Score: 839.3 bits (2167), Expect = 1.4e-239
Identity = 399/417 (95.68%), Postives = 402/417 (96.40%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ
Sbjct: 414 MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 473

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN
Sbjct: 474 AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 533

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKD IIDKVRKA+A
Sbjct: 534 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDTIIDKVRKAQA 593

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTI+VE+TPNISEAANNTLHK
Sbjct: 594 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTITVEKTPNISEAANNTLHK 653

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWST WKIALWARL N E AYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF
Sbjct: 654 RGEEGPGWSTAWKIALWARLRNSEQAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 713

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGFSAAIAEMLVQSTVKDLYLLPALP DVWSDGCVKGLKARGGLTVNICWKGGDL
Sbjct: 714 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPCDVWSDGCVKGLKARGGLTVNICWKGGDL 773

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP 413
           DEVGVWSSEQISVTTLHYRGSKVA NLTSGKLYTFNK LKC  TCSLLPNSNGFPNP
Sbjct: 774 DEVGVWSSEQISVTTLHYRGSKVAVNLTSGKLYTFNKQLKCARTCSLLPNSNGFPNP 830

[Close]

BLAST of CmoCh16G003580.1 vs. NCBI nr
Match: KAG6576933.1 (Alpha-L-fucosidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 804.3 bits (2076), Expect = 4.9e-229
Identity = 386/409 (94.38%), Postives = 389/409 (95.11%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ
Sbjct: 414 MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 473

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN
Sbjct: 474 AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 533

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKD IIDKVRKAEA
Sbjct: 534 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDTIIDKVRKAEA 593

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTI+VE+TPNISEAANNTLHK
Sbjct: 594 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTITVEKTPNISEAANNTLHK 653

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWST WKIALWARL NGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF
Sbjct: 654 RGEEGPGWSTAWKIALWARLRNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 713

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL
Sbjct: 714 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 773

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSLLP 405
           DEVGVWSSEQISVTTLHYRGSKVAANLTSG L +   LL     C L P
Sbjct: 774 DEVGVWSSEQISVTTLHYRGSKVAANLTSGLLLSDRLLLFLGFACHLSP 822

[Close]

BLAST of CmoCh16G003580.1 vs. NCBI nr
Match: KAG7014958.1 (Alpha-L-fucosidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 799.3 bits (2063), Expect = 1.6e-227
Identity = 381/398 (95.73%), Postives = 387/398 (97.24%), Query Frame = 0


Query: 16  EPLFDFTSFLSVN-GRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQAVWALWPMGGAWLC 75
           E LF ++ +L ++  R   +VNYEANGWVAHQVSDIWAKSSPDQGQAVWALWPMGGAWLC
Sbjct: 324 ELLFQYSRYLLISCSRPGTQVNYEANGWVAHQVSDIWAKSSPDQGQAVWALWPMGGAWLC 383

Query: 76  THLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETNPSTSPEHMFIAPDG 135
           THLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETNPSTSPEHMFIAPDG
Sbjct: 384 THLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETNPSTSPEHMFIAPDG 443

Query: 136 KPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEARLLPYKIARDGSLM 195
           KPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEARLLPYKIARDGSLM
Sbjct: 444 KPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEARLLPYKIARDGSLM 503

Query: 196 EWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHKRGWSTVWKIALWAR 255
           EWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHKRGWST WKIALWAR
Sbjct: 504 EWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHKRGWSTAWKIALWAR 563

Query: 256 LWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPFQIDANFGFSAAIAEMLVQS 315
           L NGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPFQIDANFGFSAAIAEMLVQS
Sbjct: 564 LRNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPFQIDANFGFSAAIAEMLVQS 623

Query: 316 TVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDLDEVGVWSSEQISVTTLHYR 375
           TVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDLDEVGVWSSEQISVTTLHYR
Sbjct: 624 TVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDLDEVGVWSSEQISVTTLHYR 683

Query: 376 GSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP 413
           GSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP
Sbjct: 684 GSKVAANLTSGKLYTFNKLLKCVGTCSLLPNSNGFPNP 721

[Close]

BLAST of CmoCh16G003580.1 vs. TAIR 10
Match: AT4G34260.1 (1,2-alpha-L-fucosidases )

HSP 1 Score: 615.9 bits (1587), Expect = 2.3e-176
Identity = 286/407 (70.27%), Postives = 341/407 (83.78%), Query Frame = 0


Query: 1   MNYWPSLPCNLKECQEPLFDFTSFLSVNGRKTAKVNYEANGWVAHQVSDIWAKSSPDQGQ 60
           MNYW SLP N++ECQEPLFD+ S L++NGRKTA+VNY A+GWVAHQVSDIWAK+SPD+G+
Sbjct: 438 MNYWHSLPGNIRECQEPLFDYMSALAINGRKTAQVNYGASGWVAHQVSDIWAKTSPDRGE 497

Query: 61  AVWALWPMGGAWLCTHLWEHYTYTMDKNFLRNKAYPLMEGCTSFLLDWLIDGKNGYLETN 120
           AVWALWPMGGAWLCTH WEHYTYTMDK FL+ K YPL+EGCTSFLLDWLI GK+G+L+TN
Sbjct: 498 AVWALWPMGGAWLCTHAWEHYTYTMDKEFLKKKGYPLLEGCTSFLLDWLIKGKDGFLQTN 557

Query: 121 PSTSPEHMFIAPDGKPASVSYSTTMDIAIIKEVFSAIIAAAEILGRSKDAIIDKVRKAEA 180
           PSTSPEHMF AP GKPASVSYS+TMDIAIIKEVF+ I++A+EILG++ D +I KV  A+A
Sbjct: 558 PSTSPEHMFTAPIGKPASVSYSSTMDIAIIKEVFADIVSASEILGKTNDTLIGKVIAAQA 617

Query: 181 RLLPYKIARDGSLMEWAIDFEDQEVHHRHVSHLFGLYPGHTISVERTPNISEAANNTLHK 240
           +L P +I++DGS+ EWA DFED EVHHRHVSHLFGL+PGHTI+VE++P +++A   TL K
Sbjct: 618 KLPPTRISKDGSIREWAEDFEDPEVHHRHVSHLFGLFPGHTITVEKSPELAKAVEATLKK 677

Query: 241 R-----GWSTVWKIALWARLWNGEHAYRMVKHLFELVDPEHESDYEGGLYSNLFTAHPPF 300
           R     GWST WK ALWARL N EHAYRMV H+F+LVDP +E +YEGGLYSN+FTAHPPF
Sbjct: 678 RGEEGPGWSTTWKAALWARLHNSEHAYRMVTHIFDLVDPLNERNYEGGLYSNMFTAHPPF 737

Query: 301 QIDANFGFSAAIAEMLVQSTVKDLYLLPALPGDVWSDGCVKGLKARGGLTVNICWKGGDL 360
           QIDANFGF+AA+AEMLVQST KDLYLLPALP D W +G V GL+ARGG+TV+I W  G+L
Sbjct: 738 QIDANFGFAAAVAEMLVQSTTKDLYLLPALPADKWPNGIVNGLRARGGVTVSIKWMEGNL 797

Query: 361 DEVGVWSSEQISVTTLHYRGSKVAANLTSGKLYTFNKLLKCVGTCSL 403
            E G+W SEQI  T + YRG   AA L  GK++TF+K L+C+ T  L
Sbjct: 798 VEFGLW-SEQIVSTRIVYRGISAAAELLPGKVFTFDKDLRCIRTDKL 843

The following BLAST results are available for this feature:

BLAST of CmoCh16G003580.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L7W8	3.3e-175	70.27	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1	[more]
Q5AU81	4.9e-46	32.89	Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112...	[more]
A2R797	1.2e-41	30.69	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513...	[more]
Q2USL3	1.8e-24	32.46	Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) O...	[more]

BLAST of CmoCh16G003580.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E566	1.0e-248	98.80	alpha-L-fucosidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430687 PE=4 SV=...	[more]
A0A6J1JA05	6.7e-240	95.68	alpha-L-fucosidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482622 PE=4 SV=1	[more]
A0A6J1IV53	1.7e-222	89.43	alpha-L-fucosidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111478832 PE=4 SV=1	[more]
A0A6J1FNV4	4.1e-221	88.45	alpha-L-fucosidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111447154 PE=4 SV=...	[more]
A0A5D3CPM6	1.7e-219	88.70	Alpha-L-fucosidase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832...	[more]

BLAST of CmoCh16G003580.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922821.1	2.1e-248	98.80	alpha-L-fucosidase 2-like [Cucurbita moschata]	[more]
XP_023551736.1	8.4e-245	97.60	alpha-L-fucosidase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022984253.1	1.4e-239	95.68	alpha-L-fucosidase 2-like [Cucurbita maxima]	[more]
KAG6576933.1	4.9e-229	94.38	Alpha-L-fucosidase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7014958.1	1.6e-227	95.73	Alpha-L-fucosidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CmoCh16G003580.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34260.1	2.3e-176	70.27	1,2-alpha-L-fucosidases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012341	Six-hairpin glycosidase-like superfamily	GENE3D	1.50.10.10		coord: 1..311 e-value: 4.5E-124 score: 417.1
None	No IPR available	PANTHER	PTHR31084:SF13	SUBFAMILY NOT NAMED	coord: 1..403
None	No IPR available	PANTHER	PTHR31084	ALPHA-L-FUCOSIDASE 2	coord: 1..403
IPR008928	Six-hairpin glycosidase superfamily	SUPERFAMILY	48208	Six-hairpin glycosidases	coord: 13..358

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh16G003580	CmoCh16G003580	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G003580.1:exon:8659	CmoCh16G003580.1:exon:8659	exon
CmoCh16G003580.1:exon:8658	CmoCh16G003580.1:exon:8658	exon
CmoCh16G003580.1:exon:8657	CmoCh16G003580.1:exon:8657	exon
CmoCh16G003580.1:exon:8656	CmoCh16G003580.1:exon:8656	exon
CmoCh16G003580.1:exon:8655	CmoCh16G003580.1:exon:8655	exon
CmoCh16G003580.1:exon:8654	CmoCh16G003580.1:exon:8654	exon
CmoCh16G003580.1:exon:8653	CmoCh16G003580.1:exon:8653	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G003580.1:three_prime_utr	CmoCh16G003580.1:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G003580.1:cds	CmoCh16G003580.1:cds_7	CDS
CmoCh16G003580.1:cds	CmoCh16G003580.1:cds_6	CDS
CmoCh16G003580.1:cds	CmoCh16G003580.1:cds_5	CDS
CmoCh16G003580.1:cds	CmoCh16G003580.1:cds_4	CDS
CmoCh16G003580.1:cds	CmoCh16G003580.1:cds_3	CDS
CmoCh16G003580.1:cds	CmoCh16G003580.1:cds_2	CDS
CmoCh16G003580.1:cds	CmoCh16G003580.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh16G003580.1	CmoCh16G003580.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004560	alpha-L-fucosidase activity