CmoCh15G006570.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh15G006570.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Calcium-binding EF-hand family protein
Location	Cmo_Chr15: 3182657 .. 3183139 (-)
Sequence length	483
RNA-Seq Expression	CmoCh15G006570.1
Synteny	CmoCh15G006570.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCTTCCTATTAGCTCAAACGACTTGCTCAGGATCTTCAACAGGCTCGACAAGAACAAGGATGGCCTCATTTGCGTTCAGGAGCTCAAGTGGCTCCTAGACACCATTGGCATTCAATTGACCATGGAGGAGTTGGAGTCGTTTCTCGAGAAACCGAGCCTTGACTTCGATGAGTTCTTGTTCTTTTACGAGTCGATATCGAAGCAAAATAAAGGCGAGTGCAAGGGTGGGTTGGTTGATGAGCATGGAGATGAAGAGGAAGATGTGGAGATTGTATATATGGCTTTTAAAGTGTTTGATGTGAATGGTGATGGCTTCATTTCTTGCGAGGAGCTCGAAAACGTGCTGTCGAGACTTGAGCTTTGGGATGCAAATAAAAGCGAGGTTGATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTCGATTTTGAGGAGTTTATGAATATGATGTTGTTCACAAACTAG

mRNA sequence

ATGGCTCTTCCTATTAGCTCAAACGACTTGCTCAGGATCTTCAACAGGCTCGACAAGAACAAGGATGGCCTCATTTGCGTTCAGGAGCTCAAGTGGCTCCTAGACACCATTGGCATTCAATTGACCATGGAGGAGTTGGAGTCGTTTCTCGAGAAACCGAGCCTTGACTTCGATGAGTTCTTGTTCTTTTACGAGTCGATATCGAAGCAAAATAAAGGCGAGTGCAAGGGTGGGTTGGTTGATGAGCATGGAGATGAAGAGGAAGATGTGGAGATTGTATATATGGCTTTTAAAGTGTTTGATGTGAATGGTGATGGCTTCATTTCTTGCGAGGAGCTCGAAAACGTGCTGTCGAGACTTGAGCTTTGGGATGCAAATAAAAGCGAGGTTGATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTCGATTTTGAGGAGTTTATGAATATGATGTTGTTCACAAACTAG

Coding sequence (CDS)

ATGGCTCTTCCTATTAGCTCAAACGACTTGCTCAGGATCTTCAACAGGCTCGACAAGAACAAGGATGGCCTCATTTGCGTTCAGGAGCTCAAGTGGCTCCTAGACACCATTGGCATTCAATTGACCATGGAGGAGTTGGAGTCGTTTCTCGAGAAACCGAGCCTTGACTTCGATGAGTTCTTGTTCTTTTACGAGTCGATATCGAAGCAAAATAAAGGCGAGTGCAAGGGTGGGTTGGTTGATGAGCATGGAGATGAAGAGGAAGATGTGGAGATTGTATATATGGCTTTTAAAGTGTTTGATGTGAATGGTGATGGCTTCATTTCTTGCGAGGAGCTCGAAAACGTGCTGTCGAGACTTGAGCTTTGGGATGCAAATAAAAGCGAGGTTGATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTCGATTTTGAGGAGTTTATGAATATGATGTTGTTCACAAACTAG

Protein sequence

MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN

Homology

[Close]

BLAST of CmoCh15G006570.1 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 132.5 bits (332), Expect = 4.3e-30
Identity = 77/154 (50.00%), Postives = 99/154 (64.29%), Query Frame = 0


Query: 5   ISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIG-IQLTMEELESFLEKPSLDFDEFLFF 64
           I++NDL R+F  LDKN+DGL+ + EL W+LD +G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 65  YESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLELW 124
           Y      +KG  K   +D   D +   E +  AF VFDVNGDG+IS EEL +VL RL   
Sbjct: 66  YYDAVLDSKGSKKN--IDVVADND---EAIARAFNVFDVNGDGYISAEELRDVLERLGFE 125

Query: 125 DANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMML 158
           +  K+  DC  MIR +D NLDG +DFEEF NM+L
Sbjct: 126 EEAKA-WDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of CmoCh15G006570.1 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 4.9e-18
Identity = 61/155 (39.35%), Postives = 85/155 (54.84%), Query Frame = 0


Query: 10  LLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLE------KPSLDFDEFLFF 69
           L RIF+  DKN DG I V+EL   L  +G+   + +L+S +E         L+FD+F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 70  YESISKQ-NKGECKGG-LVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLE 129
           ++++      G C GG   D+     E+   +  AFKVFD NGDGFIS  EL+ VL +L 
Sbjct: 90  HKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLG 149

Query: 130 LWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
           L +  + E     MI + D N DG++DF EF NMM
Sbjct: 150 LPEGGEME-RVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of CmoCh15G006570.1 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-17
Identity = 60/154 (38.96%), Postives = 86/154 (55.84%), Query Frame = 0


Query: 9   DLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEK------PSLDFDEFLF 68
           +L R+F   DKN DG I  +EL   L ++GI +  +EL   +EK        +D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEF-- 64

Query: 69  FYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 128
                     GE    ++DE  +EEED++    AF VFD NGDGFI+ +EL+ VLS L L
Sbjct: 65  ----------GELYKTIMDEEDEEEEDMK---EAFNVFDQNGDGFITVDELKAVLSSLGL 124

Query: 129 WDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
               K+  DC+ MI+  D + DG+++++EF  MM
Sbjct: 125 -KQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of CmoCh15G006570.1 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.1e-17
Identity = 60/153 (39.22%), Postives = 86/153 (56.21%), Query Frame = 0


Query: 10  LLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFL------EKPSLDFDEFLFF 69
           L R+F+  DKN DG I V+EL   L  +G+     +L+S +      +K  L FD+F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 70  YESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLELW 129
           ++++ +   G  +G   D  G  E D+E    AF VFD +GDGFIS  EL+ VL +L L 
Sbjct: 89  HKTLDESFFGG-EGSCCD--GSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGLP 148

Query: 130 DANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
           +A + E     MI + D+N DG++DF EF NMM
Sbjct: 149 EAGEIE-QVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of CmoCh15G006570.1 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.0e-16
Identity = 56/154 (36.36%), Postives = 79/154 (51.30%), Query Frame = 0


Query: 9   DLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEK------PSLDFDEFLF 68
           +L R+F   DKN DG I   ELK    ++GI +   E+   + K       ++D DEF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF-- 64

Query: 69  FYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 128
                         G L  E  +E+E+ E +  AF+VFD NGDGFI+ EEL +VL+ + L
Sbjct: 65  --------------GSLYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGL 124

Query: 129 WDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
                 E DC+ MI   D + DG ++F+EF  MM
Sbjct: 125 KQGRTLE-DCKKMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of CmoCh15G006570.1 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 9.7e-86
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmoCh15G006570.1 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.9e-81
Identity = 153/160 (95.62%), Postives = 156/160 (97.50%), Query Frame = 0


Query: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MA PISSNDLLRIFNRLDKN DGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           L FYES+SKQNKGECKGGLVDEH DE+EDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDAN+SEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmoCh15G006570.1 vs. ExPASy TrEMBL
Match: A0A5D3B6U1 (Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold282G00080 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.9e-66
Identity = 131/158 (82.91%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFLFF
Sbjct: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62

Query: 64  YESISKQNKGECKGGLVD-EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123
           YESISKQNKGECKGG+      D E+D EIVYMAFKVFD+NGDGFISC+ELENVL RLEL
Sbjct: 63  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 122

Query: 124 WDANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 160
           WDA++SEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 123 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 160

[Close]

BLAST of CmoCh15G006570.1 vs. ExPASy TrEMBL
Match: A0A1S3B3T2 (probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.9e-66
Identity = 131/158 (82.91%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFLFF
Sbjct: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62

Query: 64  YESISKQNKGECKGGLVD-EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123
           YESISKQNKGECKGG+      D E+D EIVYMAFKVFD+NGDGFISC+ELENVL RLEL
Sbjct: 63  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 122

Query: 124 WDANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 160
           WDA++SEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 123 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 160

[Close]

BLAST of CmoCh15G006570.1 vs. ExPASy TrEMBL
Match: A0A0A0KFQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.6e-64
Identity = 128/162 (79.01%), Postives = 144/162 (88.89%), Query Frame = 0


Query: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63
           PISS DL RIF +LDKN DGLIC+QEL WLLD+IGIQLTMEELESFLE+PSLDFDEFLFF
Sbjct: 3   PISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFLFF 62

Query: 64  YESISKQNKGECKGGLV----DEHGDE-EEDVEIVYMAFKVFDVNGDGFISCEELENVLS 123
           YESISKQNKGECKGG+     D   D+ ++D+EIVY+AFKVFD+NGDGFISC+ELENVL 
Sbjct: 63  YESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENVLV 122

Query: 124 RLELWDANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 160
           +LELWDA++S+VD CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 123 KLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 164

[Close]

BLAST of CmoCh15G006570.1 vs. NCBI nr
Match: XP_022939513.1 (probable calcium-binding protein CML44 [Cucurbita moschata] >KAG7016457.1 putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 325.9 bits (834), Expect = 2.0e-85
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmoCh15G006570.1 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 324.7 bits (831), Expect = 4.5e-85
Identity = 159/160 (99.38%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDAN+SEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmoCh15G006570.1 vs. NCBI nr
Match: KAG6578937.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 321.2 bits (822), Expect = 4.9e-84
Identity = 158/160 (98.75%), Postives = 159/160 (99.38%), Query Frame = 0


Query: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           LFFYESISKQNKGECKGGLV EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LFFYESISKQNKGECKGGLVYEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDANK+EVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANKNEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmoCh15G006570.1 vs. NCBI nr
Match: XP_022993348.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 311.6 bits (797), Expect = 3.9e-81
Identity = 153/160 (95.62%), Postives = 156/160 (97.50%), Query Frame = 0


Query: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MA PISSNDLLRIFNRLDKN DGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           L FYES+SKQNKGECKGGLVDEH DE+EDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDAN+SEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmoCh15G006570.1 vs. NCBI nr
Match: XP_038885742.1 (probable calcium-binding protein CML44 [Benincasa hispida])

HSP 1 Score: 263.5 bits (672), Expect = 1.2e-66
Identity = 130/156 (83.33%), Postives = 142/156 (91.03%), Query Frame = 0


Query: 5   ISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFFY 64
           IS NDL RIFN+LDKN DGLIC+QELKWL D IGIQLTMEELESFLEKPSLDF+EFLFFY
Sbjct: 14  ISFNDLHRIFNKLDKNGDGLICLQELKWLFDRIGIQLTMEELESFLEKPSLDFNEFLFFY 73

Query: 65  ESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLELWD 124
           ESISKQNKGECKGG+     D EE++EIVYMAFKVFD+NGDGFISC+ELENVLSRLELWD
Sbjct: 74  ESISKQNKGECKGGVGCLQDDHEEEMEIVYMAFKVFDMNGDGFISCDELENVLSRLELWD 133

Query: 125 ANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 160
           AN++++D CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 134 ANRNDIDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 169

[Close]

BLAST of CmoCh15G006570.1 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 132.5 bits (332), Expect = 3.0e-31
Identity = 77/154 (50.00%), Postives = 99/154 (64.29%), Query Frame = 0


Query: 5   ISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIG-IQLTMEELESFLEKPSLDFDEFLFF 64
           I++NDL R+F  LDKN+DGL+ + EL W+LD +G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 65  YESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLELW 124
           Y      +KG  K   +D   D +   E +  AF VFDVNGDG+IS EEL +VL RL   
Sbjct: 66  YYDAVLDSKGSKKN--IDVVADND---EAIARAFNVFDVNGDGYISAEELRDVLERLGFE 125

Query: 125 DANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMML 158
           +  K+  DC  MIR +D NLDG +DFEEF NM+L
Sbjct: 126 EEAKA-WDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of CmoCh15G006570.1 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 92.4 bits (228), Expect = 3.5e-19
Identity = 61/155 (39.35%), Postives = 85/155 (54.84%), Query Frame = 0


Query: 10  LLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLE------KPSLDFDEFLFF 69
           L RIF+  DKN DG I V+EL   L  +G+   + +L+S +E         L+FD+F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 70  YESISKQ-NKGECKGG-LVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLE 129
           ++++      G C GG   D+     E+   +  AFKVFD NGDGFIS  EL+ VL +L 
Sbjct: 90  HKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLG 149

Query: 130 LWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
           L +  + E     MI + D N DG++DF EF NMM
Sbjct: 150 LPEGGEME-RVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of CmoCh15G006570.1 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 91.3 bits (225), Expect = 7.7e-19
Identity = 60/154 (38.96%), Postives = 86/154 (55.84%), Query Frame = 0


Query: 9   DLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEK------PSLDFDEFLF 68
           +L R+F   DKN DG I  +EL   L ++GI +  +EL   +EK        +D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEF-- 64

Query: 69  FYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 128
                     GE    ++DE  +EEED++    AF VFD NGDGFI+ +EL+ VLS L L
Sbjct: 65  ----------GELYKTIMDEEDEEEEDMK---EAFNVFDQNGDGFITVDELKAVLSSLGL 124

Query: 129 WDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
               K+  DC+ MI+  D + DG+++++EF  MM
Sbjct: 125 -KQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of CmoCh15G006570.1 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 89.4 bits (220), Expect = 2.9e-18
Identity = 60/153 (39.22%), Postives = 86/153 (56.21%), Query Frame = 0


Query: 10  LLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFL------EKPSLDFDEFLFF 69
           L R+F+  DKN DG I V+EL   L  +G+     +L+S +      +K  L FD+F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 70  YESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLELW 129
           ++++ +   G  +G   D  G  E D+E    AF VFD +GDGFIS  EL+ VL +L L 
Sbjct: 89  HKTLDESFFGG-EGSCCD--GSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGLP 148

Query: 130 DANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
           +A + E     MI + D+N DG++DF EF NMM
Sbjct: 149 EAGEIE-QVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of CmoCh15G006570.1 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 87.0 bits (214), Expect = 1.5e-17
Identity = 56/154 (36.36%), Postives = 79/154 (51.30%), Query Frame = 0


Query: 9   DLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEK------PSLDFDEFLF 68
           +L R+F   DKN DG I   ELK    ++GI +   E+   + K       ++D DEF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF-- 64

Query: 69  FYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 128
                         G L  E  +E+E+ E +  AF+VFD NGDGFI+ EEL +VL+ + L
Sbjct: 65  --------------GSLYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGL 124

Query: 129 WDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
                 E DC+ MI   D + DG ++F+EF  MM
Sbjct: 125 KQGRTLE-DCKKMISKVDVDGDGMVNFKEFKQMM 141

The following BLAST results are available for this feature:

BLAST of CmoCh15G006570.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPK5	4.3e-30	50.00	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ...	[more]
Q9SVG9	4.9e-18	39.35	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q9LNE7	1.1e-17	38.96	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9FI19	4.1e-17	39.22	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q9SU00	2.0e-16	36.36	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]

BLAST of CmoCh15G006570.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FLU5	9.7e-86	100.00	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1K1Y7	1.9e-81	95.63	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893...	[more]
A0A5D3B6U1	2.9e-66	82.91	Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3B3T2	2.9e-66	82.91	probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 P...	[more]
A0A0A0KFQ6	1.6e-64	79.01	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1	[more]

BLAST of CmoCh15G006570.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022939513.1	2.0e-85	100.00	probable calcium-binding protein CML44 [Cucurbita moschata] >KAG7016457.1 putati...	[more]
XP_023551499.1	4.5e-85	99.38	probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo]	[more]
KAG6578937.1	4.9e-84	98.75	putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022993348.1	3.9e-81	95.63	probable calcium-binding protein CML44 [Cucurbita maxima]	[more]
XP_038885742.1	1.2e-66	83.33	probable calcium-binding protein CML44 [Benincasa hispida]	[more]

BLAST of CmoCh15G006570.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21550.1	3.0e-31	50.00	Calcium-binding EF-hand family protein	[more]
AT4G20780.1	3.5e-19	39.35	calmodulin like 42	[more]
AT1G05990.1	7.7e-19	38.96	EF hand calcium-binding protein family	[more]
AT5G44460.1	2.9e-18	39.22	calmodulin like 43	[more]
AT4G12860.1	1.5e-17	36.36	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 131..159 e-value: 0.17 score: 21.0 coord: 9..37 e-value: 0.0062 score: 25.7 coord: 92..120 e-value: 0.0022 score: 27.2
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 13..31 e-value: 8.7E-5 score: 21.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 94..156 e-value: 3.1E-12 score: 46.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 88..123 score: 13.286007
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 5..40 score: 12.086506
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 127..160 score: 13.174425
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 96..156 e-value: 6.71051E-13 score: 58.3281
None	No IPR available	GENE3D	1.10.238.10		coord: 5..64 e-value: 6.9E-10 score: 40.9
None	No IPR available	GENE3D	1.10.238.10		coord: 65..156 e-value: 5.7E-20 score: 73.4
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 9..160
None	No IPR available	PANTHER	PTHR10891:SF788	CALCIUM-BINDING PROTEIN CML44-RELATED	coord: 9..160
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 101..113
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 18..30
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 9..157

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh15G006570	CmoCh15G006570	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh15G006570.1:exon:4343	CmoCh15G006570.1:exon:4343	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh15G006570.1:cds	CmoCh15G006570.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh15G006570.1	CmoCh15G006570.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding