CmoCh14G012810.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh14G012810.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionankyrin repeat-containing protein NPR4-like isoform X3
LocationCmo_Chr14: 10727693 .. 10728885 (-)
Sequence length606
RNA-Seq ExpressionCmoCh14G012810.1
SyntenyCmoCh14G012810.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCAAGATCTCAAACATCCCACGTCCTCTTTGAATGCTATTCCTGATGATGATGCACATAATCAAGAAATTAGAATTGTGATGCAGTCTGAGGTTGATGCCTTTCAACCAACGCCCCTCCACCTTGCTTCCAAGAATGGAGACATCGACACCGTTCGAGCTTTGTTGGAGAACAACACGAGTGCTTGCTTGGTTTACGATAACAACGGTTTCATTCCTCTCCATTATGCAGTAATTAATGGACAAGTCGAGATCACGAAAGAGCTTATCAATGCAACCCCACAATCTATTTGGATGAAGCTTAAAGATGGTCGAACTGCCTTGCATTTATGCGTAGAAAGTAACCATTTAGAGAGTCTAAAGCTGCTTATTGAAACATTTATGAATCGTCACGAAGGTTTCTTGAATACAACTGATGACGAGGGGAACACTATTTTGGATTTATCAATCAAACTCAGACAAAGTGAGGTAAGTAATAAACAAAAACTCTAAGGGTATAAAGATATTTGTCTTCCATTGCTGCTCACCATAGGTATCATATCTTGTATCTGTTTATTGCTATTTTCATTTATTGTTATTTCCATATCTTTGTAATTTATTTATTTTAATGCAGATGGTAGGATATTTACTCTCACTCTCTGAAGTAAAAACTAGAATAAGCACAACTTCAAATGTTAGAAAAAGAAGTCTAGAATCTCGAAATTCTAAAAAACAAAGACGAGAATCTGCATCATTTAGAATAGGGCGGTGGAAGGCATGGAGGAAGAAATTAAAATACAAAGGAGATTGGGTTCAAGAAGTGCAAGGGACAATGATGTTGGTGGGTACAGTAATCGCAACCGTGACTTTTCAAGCTGGGGTGAACCCTGCAGGCGGTGTTTGGCAAGAAGACACTCCAGACAGTGCTACTTTGAAAAATGGGACAATTCACCGAGCTGGAAGTGCAATTATGGCATACCGACTACCAGTAGAGCACTTAATTTACTCAATGGCCAACGTGGTCTCGTTCATGGCCTCTGTAAGCGTGATTCTGCTAATCATCAGTCGAGTTCCACTAAAAAATAGAGTTTGTAGTTGGGTGTTAGTGTTGGCCATGTGTGTGGCAGTAGTGTTCTTAGCATTGGCCTTTCTACAAGGATTTATAATGGTTAACGTCGACAATCTTGCTATAGGATTTGGATATTATTTAG

mRNA sequence

ATGGAGCAAGATCTCAAACATCCCACGTCCTCTTTGAATGCTATTCCTGATGATGATGCACATAATCAAGAAATTAGAATTGTGATGCAGTCTGAGGTTGATGCCTTTCAACCAACGCCCCTCCACCTTGCTTCCAAGAATGGAGACATCGACACCGTTCGAGCTTTGTTGGAGAACAACACGAGTGCTTGCTTGGTTTACGATAACAACGGTTTCATTCCTCTCCATTATGCAGTAATTAATGGACAAGTCGAGATCACGAAAGAGCTTATCAATGCAACCCCACAATCTATTTGGATGAAGCTTAAAGATGGTCGAACTGCCTTGCATTTATGCGTAGAAAGTAACCATTTAGAGAGTCTAAAGCTGCTTATTGAAACATTTATGAATCGTCACGAAGTAATCGCAACCGTGACTTTTCAAGCTGGGGTGAACCCTGCAGGCGGTGTTTGGCAAGAAGACACTCCAGACAGTGCTACTTTGAAAAATGGGACAATTCACCGAGCTGGAAGTGCAATTATGGCATACCGACTACCAGTAGAGCACTTAATTTACTCAATGGCCAACGTGGTCTCGTTCATGGCCTCTGATTTGGATATTATTTAG

Coding sequence (CDS)

ATGGAGCAAGATCTCAAACATCCCACGTCCTCTTTGAATGCTATTCCTGATGATGATGCACATAATCAAGAAATTAGAATTGTGATGCAGTCTGAGGTTGATGCCTTTCAACCAACGCCCCTCCACCTTGCTTCCAAGAATGGAGACATCGACACCGTTCGAGCTTTGTTGGAGAACAACACGAGTGCTTGCTTGGTTTACGATAACAACGGTTTCATTCCTCTCCATTATGCAGTAATTAATGGACAAGTCGAGATCACGAAAGAGCTTATCAATGCAACCCCACAATCTATTTGGATGAAGCTTAAAGATGGTCGAACTGCCTTGCATTTATGCGTAGAAAGTAACCATTTAGAGAGTCTAAAGCTGCTTATTGAAACATTTATGAATCGTCACGAAGTAATCGCAACCGTGACTTTTCAAGCTGGGGTGAACCCTGCAGGCGGTGTTTGGCAAGAAGACACTCCAGACAGTGCTACTTTGAAAAATGGGACAATTCACCGAGCTGGAAGTGCAATTATGGCATACCGACTACCAGTAGAGCACTTAATTTACTCAATGGCCAACGTGGTCTCGTTCATGGCCTCTGATTTGGATATTATTTAG

Protein sequence

MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENNTSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLESLKLLIETFMNRHEVIATVTFQAGVNPAGGVWQEDTPDSATLKNGTIHRAGSAIMAYRLPVEHLIYSMANVVSFMASDLDII
Homology
BLAST of CmoCh14G012810.1 vs. ExPASy Swiss-Prot
Match: P16157 (Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3)

HSP 1 Score: 63.9 bits (154), Expect = 2.3e-09
Identity = 45/138 (32.61%), Postives = 66/138 (47.83%), Query Frame = 0

Query: 39  TPLHLASKNGDIDTVRALLENNTS-ACLVYDNNGFIPLHYAVINGQVEITKELI--NATP 98
           TPLH+A++ G ++TV ALLE   S AC+     GF PLH A   G+V + + L+  +A P
Sbjct: 505 TPLHIAAREGHVETVLALLEKEASQACMT--KKGFTPLHVAAKYGKVRVAELLLERDAHP 564

Query: 99  QSIWMKLKDGRTALHLCVESNHLESLKLLIETFMNRHEVIATVTFQAGVNPAGGVWQEDT 158
            +     K+G T LH+ V  N+L+ +KLL+                 G +P    W   T
Sbjct: 565 NAAG---KNGLTPLHVAVHHNNLDIVKLLL---------------PRGGSPHSPAWNGYT 622

Query: 159 PDSATLKNGTIHRAGSAI 174
           P     K   +  A S +
Sbjct: 625 PLHIAAKQNQVEVARSLL 622

BLAST of CmoCh14G012810.1 vs. ExPASy Swiss-Prot
Match: Q02357 (Ankyrin-1 OS=Mus musculus OX=10090 GN=Ank1 PE=1 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 5.2e-09
Identity = 46/138 (33.33%), Postives = 64/138 (46.38%), Query Frame = 0

Query: 39  TPLHLASKNGDIDTVRALLENNTS-ACLVYDNNGFIPLHYAVINGQVEITKELI--NATP 98
           TPLH A++ G +DT  ALLE   S AC+     GF PLH A   G+V + + L+  +A P
Sbjct: 501 TPLHTAAREGHVDTALALLEKEASQACMT--KKGFTPLHVAAKYGKVRLAELLLEHDAHP 560

Query: 99  QSIWMKLKDGRTALHLCVESNHLESLKLLIETFMNRHEVIATVTFQAGVNPAGGVWQEDT 158
            +     K+G T LH+ V  N+L+ +KLL+                 G +P    W   T
Sbjct: 561 NAAG---KNGLTPLHVAVHHNNLDIVKLLL---------------PRGGSPHSPAWNGYT 618

Query: 159 PDSATLKNGTIHRAGSAI 174
           P     K   I  A S +
Sbjct: 621 PLHIAAKQNQIEVARSLL 618

BLAST of CmoCh14G012810.1 vs. ExPASy Swiss-Prot
Match: Q18297 (Transient receptor potential cation channel subfamily A member 1 homolog OS=Caenorhabditis elegans OX=6239 GN=trpa-1 PE=2 SV=5)

HSP 1 Score: 59.3 bits (142), Expect = 5.7e-08
Identity = 37/122 (30.33%), Postives = 63/122 (51.64%), Query Frame = 0

Query: 5   LKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENNTSAC 64
           LK PT +   I  +D     +R  M + VD  Q TP+H+ + NG ++ ++ L ++  S  
Sbjct: 447 LKSPTRNTLRIVSED-----VRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASIT 506

Query: 65  LVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLESLKLL 124
            V ++     LH A I GQ    ++L+    + + MK + G +ALHL   S H  + K+L
Sbjct: 507 QVNEDEE-TALHRAAIGGQTGAVRQLLEWDIRLLLMKDEMGNSALHLAARSGHDATTKVL 562

Query: 125 IE 127
           ++
Sbjct: 567 LD 562

BLAST of CmoCh14G012810.1 vs. ExPASy Swiss-Prot
Match: Q8N7Z5 (Ankyrin repeat domain-containing protein 31 OS=Homo sapiens OX=9606 GN=ANKRD31 PE=2 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 8.3e-07
Identity = 32/93 (34.41%), Postives = 54/93 (58.06%), Query Frame = 0

Query: 41   LHLASKNGDIDTVRALLENNTSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWM 100
            LHLA + G++  V+AL+E+     L  DN G+ PLH A   G ++I  EL+ A  + +  
Sbjct: 1159 LHLAVRRGNLPLVKALIESGADVNL-NDNAGWTPLHEASNEGSIDIIVELLKAGAK-VNC 1218

Query: 101  KLKDGRTALHLCVESNHLESLKLLIETFMNRHE 134
            +  DG   LH  V +NHL++ ++L++   N ++
Sbjct: 1219 ENIDGILPLHDAVANNHLKAAEILLQNGANPNQ 1249

BLAST of CmoCh14G012810.1 vs. ExPASy Swiss-Prot
Match: P0DJE3 (Alpha-latrotoxin-Lhe1a OS=Latrodectus hesperus OX=256737 PE=1 SV=2)

HSP 1 Score: 55.1 bits (131), Expect = 1.1e-06
Identity = 31/99 (31.31%), Postives = 50/99 (50.51%), Query Frame = 0

Query: 27  IVMQSEVDAFQPTPLHLASKNGDIDTVRALLENNTSACLVYDNNGFIPLHYAVINGQVEI 86
           + + S+   F  TPLHLA++ G ++T + L+E++       D +GF PLHYAV  G+  I
Sbjct: 550 VSINSKTYHFLQTPLHLAAQRGFVNTFQRLMESSEININERDKDGFTPLHYAVRGGE-RI 609

Query: 87  TKELINATPQSIWMKLKDGRTALHLCVESNHLESLKLLI 126
            +  +N     +  K   G T  HL +  N  +    L+
Sbjct: 610 LEAFMNQIGIDVNAKSNKGLTPFHLAIIKNDWQVASTLL 647

BLAST of CmoCh14G012810.1 vs. ExPASy TrEMBL
Match: A0A6J1GU04 (ankyrin repeat-containing protein NPR4-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111457477 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.1e-94
Identity = 196/292 (67.12%), Postives = 196/292 (67.12%), Query Frame = 0

Query: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60
           MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN
Sbjct: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60

Query: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120
           TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES
Sbjct: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120

Query: 121 LKLLIETFMNRHE----------------------------------------------- 180
           LKLLIETFMNRHE                                               
Sbjct: 121 LKLLIETFMNRHEGFLNTTDDEGNTILDLSIKLRQSEMVGYLLSLSEVKTRISTTSNVRK 180

Query: 181 -------------------------------------------------VIATVTFQAGV 197
                                                            VIATVTFQAGV
Sbjct: 181 RSLESRNSKKQRRESASFRIGRWKAWRKKLKYKGDWVQEVQGTMMLVGTVIATVTFQAGV 240

BLAST of CmoCh14G012810.1 vs. ExPASy TrEMBL
Match: A0A6J1GTP7 (uncharacterized protein LOC111457477 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457477 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 4.9e-87
Identity = 189/305 (61.97%), Postives = 192/305 (62.95%), Query Frame = 0

Query: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60
           MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN
Sbjct: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60

Query: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120
           TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES
Sbjct: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120

Query: 121 LKLLIETFMNRHE----------------------------------------------- 180
           LKLLIETFMNRHE                                               
Sbjct: 121 LKLLIETFMNRHEGFLNTTDDEGNTILDLSIKLRQSEMVGYLLSLSEVKTRISTSNVRKR 180

Query: 181 ------------------------------------------------VIATVTFQAGVN 197
                                                           VIATVTFQAGVN
Sbjct: 181 SLASRNSKKQRQESASFRIGRWKTWRKKLKYKGDWVQEVEGTMMLVATVIATVTFQAGVN 240

BLAST of CmoCh14G012810.1 vs. ExPASy TrEMBL
Match: A0A6J1IU53 (ankyrin repeat-containing protein NPR4-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111479945 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.1e-81
Identity = 174/291 (59.79%), Postives = 183/291 (62.89%), Query Frame = 0

Query: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60
           MEQ+LKHPT S NAIPDD A NQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN
Sbjct: 1   MEQNLKHPTPSFNAIPDDAAQNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60

Query: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120
           TSACLVYDNNGFIPLHYAVINGQVEI KELINA PQSIWMKL DGRT LHLCVE +HLES
Sbjct: 61  TSACLVYDNNGFIPLHYAVINGQVEIMKELINARPQSIWMKLNDGRTVLHLCVEGDHLES 120

Query: 121 LKLLIETFMNRHE----------------------------------------------- 180
           +KLLIETFMNRHE                                               
Sbjct: 121 IKLLIETFMNRHEGFLNTTDDKGNTILDLSTTLRKSEMVGYLLSLSEVKTRINTPNVRKR 180

Query: 181 ------------------------------------------------VIATVTFQAGVN 197
                                                           VIATVTFQAGVN
Sbjct: 181 SLASQNSTKQRRESASFRVGRWKTWRKKLKYKGDWVQEVQGIMMLVATVIATVTFQAGVN 240

BLAST of CmoCh14G012810.1 vs. ExPASy TrEMBL
Match: A0A6J1IZU6 (ankyrin repeat-containing protein NPR4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479945 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.4e-78
Identity = 174/303 (57.43%), Postives = 183/303 (60.40%), Query Frame = 0

Query: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60
           MEQ+LKHPT S NAIPDD A NQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN
Sbjct: 1   MEQNLKHPTPSFNAIPDDAAQNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60

Query: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120
           TSACLVYDNNGFIPLHYAVINGQVEI KELINA PQSIWMKL DGRT LHLCVE +HLES
Sbjct: 61  TSACLVYDNNGFIPLHYAVINGQVEIMKELINARPQSIWMKLNDGRTVLHLCVEGDHLES 120

Query: 121 LKLLIETFMNRHE----------------------------------------------- 180
           +KLLIETFMNRHE                                               
Sbjct: 121 IKLLIETFMNRHEGFLNTTDDKGNTILDLSTTLRKSEMVGYLLSLSEVKTRINTPNVRKR 180

Query: 181 ------------------------------------------------VIATVTFQAGVN 197
                                                           VIATVTFQAGVN
Sbjct: 181 SLASQNSTKQRRESASFRVGRWKTWRKKLKYKGDWVQEVQGIMMLVATVIATVTFQAGVN 240

BLAST of CmoCh14G012810.1 vs. ExPASy TrEMBL
Match: A0A6J1IX06 (ankyrin repeat-containing protein NPR4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479945 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.4e-78
Identity = 174/303 (57.43%), Postives = 183/303 (60.40%), Query Frame = 0

Query: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60
           MEQ+LKHPT S NAIPDD A NQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN
Sbjct: 1   MEQNLKHPTPSFNAIPDDAAQNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60

Query: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120
           TSACLVYDNNGFIPLHYAVINGQVEI KELINA PQSIWMKL DGRT LHLCVE +HLES
Sbjct: 61  TSACLVYDNNGFIPLHYAVINGQVEIMKELINARPQSIWMKLNDGRTVLHLCVEGDHLES 120

Query: 121 LKLLIETFMNRHE----------------------------------------------- 180
           +KLLIETFMNRHE                                               
Sbjct: 121 IKLLIETFMNRHEGFLNTTDDKGNTILDLSTTLRKSEMVGYLLSLSEVKTRINTPNVRKR 180

Query: 181 ------------------------------------------------VIATVTFQAGVN 197
                                                           VIATVTFQAGVN
Sbjct: 181 SLASQNSTKQRRESASFRVGRWKTWRKKLKYKGDWVQEVQGIMMLVATVIATVTFQAGVN 240

BLAST of CmoCh14G012810.1 vs. NCBI nr
Match: XP_022955458.1 (ankyrin repeat-containing protein NPR4-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 355.9 bits (912), Expect = 2.3e-94
Identity = 196/292 (67.12%), Postives = 196/292 (67.12%), Query Frame = 0

Query: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60
           MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN
Sbjct: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60

Query: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120
           TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES
Sbjct: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120

Query: 121 LKLLIETFMNRHE----------------------------------------------- 180
           LKLLIETFMNRHE                                               
Sbjct: 121 LKLLIETFMNRHEGFLNTTDDEGNTILDLSIKLRQSEMVGYLLSLSEVKTRISTTSNVRK 180

Query: 181 -------------------------------------------------VIATVTFQAGV 197
                                                            VIATVTFQAGV
Sbjct: 181 RSLESRNSKKQRRESASFRIGRWKAWRKKLKYKGDWVQEVQGTMMLVGTVIATVTFQAGV 240

BLAST of CmoCh14G012810.1 vs. NCBI nr
Match: XP_023528989.1 (ankyrin repeat-containing protein NPR4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 334.7 bits (857), Expect = 5.4e-88
Identity = 183/292 (62.67%), Postives = 188/292 (64.38%), Query Frame = 0

Query: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60
           MEQDLKHPT S NAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALL+NN
Sbjct: 1   MEQDLKHPTPSFNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLKNN 60

Query: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120
           T AC VYDN+GFIPLHYAVINGQV+I KELINATPQSIWMKLKDGRT LHLCVESNHLE 
Sbjct: 61  TGACFVYDNDGFIPLHYAVINGQVDIMKELINATPQSIWMKLKDGRTVLHLCVESNHLEG 120

Query: 121 LKLLIETFMNRHE----------------------------------------------- 180
           LKLLIETFMN HE                                               
Sbjct: 121 LKLLIETFMNCHEGFLNTTDDEGNTILDLSIKLRQSEMVGYLLSLSEVKTRVSTTSNVRK 180

Query: 181 -------------------------------------------------VIATVTFQAGV 197
                                                            VIATVTFQAGV
Sbjct: 181 RSLESRNSKKQRRESASFRIGRWKAWRKKLKYKGDWVQEVQGTMMLVATVIATVTFQAGV 240

BLAST of CmoCh14G012810.1 vs. NCBI nr
Match: XP_023528988.1 (ankyrin repeat-containing protein NPR4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 334.7 bits (857), Expect = 5.4e-88
Identity = 183/292 (62.67%), Postives = 188/292 (64.38%), Query Frame = 0

Query: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60
           MEQDLKHPT S NAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALL+NN
Sbjct: 1   MEQDLKHPTPSFNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLKNN 60

Query: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120
           T AC VYDN+GFIPLHYAVINGQV+I KELINATPQSIWMKLKDGRT LHLCVESNHLE 
Sbjct: 61  TGACFVYDNDGFIPLHYAVINGQVDIMKELINATPQSIWMKLKDGRTVLHLCVESNHLEG 120

Query: 121 LKLLIETFMNRHE----------------------------------------------- 180
           LKLLIETFMN HE                                               
Sbjct: 121 LKLLIETFMNCHEGFLNTTDDEGNTILDLSIKLRQSEMVGYLLSLSEVKTRVSTTSNVRK 180

Query: 181 -------------------------------------------------VIATVTFQAGV 197
                                                            VIATVTFQAGV
Sbjct: 181 RSLESRNSKKQRRESASFRIGRWKAWRKKLKYKGDWVQEVQGTMMLVATVIATVTFQAGV 240

BLAST of CmoCh14G012810.1 vs. NCBI nr
Match: XP_022955457.1 (uncharacterized protein LOC111457477 isoform X2 [Cucurbita moschata])

HSP 1 Score: 330.5 bits (846), Expect = 1.0e-86
Identity = 189/305 (61.97%), Postives = 192/305 (62.95%), Query Frame = 0

Query: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60
           MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN
Sbjct: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60

Query: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120
           TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES
Sbjct: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120

Query: 121 LKLLIETFMNRHE----------------------------------------------- 180
           LKLLIETFMNRHE                                               
Sbjct: 121 LKLLIETFMNRHEGFLNTTDDEGNTILDLSIKLRQSEMVGYLLSLSEVKTRISTSNVRKR 180

Query: 181 ------------------------------------------------VIATVTFQAGVN 197
                                                           VIATVTFQAGVN
Sbjct: 181 SLASRNSKKQRQESASFRIGRWKTWRKKLKYKGDWVQEVEGTMMLVATVIATVTFQAGVN 240

BLAST of CmoCh14G012810.1 vs. NCBI nr
Match: XP_022980641.1 (ankyrin repeat-containing protein NPR4-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 311.2 bits (796), Expect = 6.4e-81
Identity = 174/291 (59.79%), Postives = 183/291 (62.89%), Query Frame = 0

Query: 1   MEQDLKHPTSSLNAIPDDDAHNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60
           MEQ+LKHPT S NAIPDD A NQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN
Sbjct: 1   MEQNLKHPTPSFNAIPDDAAQNQEIRIVMQSEVDAFQPTPLHLASKNGDIDTVRALLENN 60

Query: 61  TSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSIWMKLKDGRTALHLCVESNHLES 120
           TSACLVYDNNGFIPLHYAVINGQVEI KELINA PQSIWMKL DGRT LHLCVE +HLES
Sbjct: 61  TSACLVYDNNGFIPLHYAVINGQVEIMKELINARPQSIWMKLNDGRTVLHLCVEGDHLES 120

Query: 121 LKLLIETFMNRHE----------------------------------------------- 180
           +KLLIETFMNRHE                                               
Sbjct: 121 IKLLIETFMNRHEGFLNTTDDKGNTILDLSTTLRKSEMVGYLLSLSEVKTRINTPNVRKR 180

Query: 181 ------------------------------------------------VIATVTFQAGVN 197
                                                           VIATVTFQAGVN
Sbjct: 181 SLASQNSTKQRRESASFRVGRWKTWRKKLKYKGDWVQEVQGIMMLVATVIATVTFQAGVN 240

BLAST of CmoCh14G012810.1 vs. TAIR 10
Match: AT5G51160.1 (Ankyrin repeat family protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.3e-10
Identity = 32/99 (32.32%), Postives = 57/99 (57.58%), Query Frame = 0

Query: 39  TPLHLASKNGDIDTVRALLENNTSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSI 98
           +PLH A+  G ++TVRA L      C + D +G  PLH A + G++++ +E++ +    +
Sbjct: 49  SPLHAAAAAGQVETVRATLGVEKKLCRLKDRDGKTPLHVATMRGKIDVIREIVASCVDCL 108

Query: 99  WMKLKDGRTALHLCVESNHLESLKLLIE--TFMNRHEVI 136
             +   G+TALHL V    +E++  ++E  T  NR +V+
Sbjct: 109 EDETVQGQTALHLAVLHLEIEAVIAIVELITETNRFDVL 147

BLAST of CmoCh14G012810.1 vs. TAIR 10
Match: AT1G07710.1 (Ankyrin repeat family protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.4e-09
Identity = 33/91 (36.26%), Postives = 47/91 (51.65%), Query Frame = 0

Query: 39  TPLHLASKNGDIDTVRALLENNTSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSI 98
           T LH AS+NG +  ++ALL +  +  +  D  G   LH AV    VE+ +ELI A   SI
Sbjct: 171 TALHSASRNGHVKVIKALLASEPAIAIRMDKKGQTALHMAVKGTNVEVVEELIKADRSSI 230

Query: 99  WMKLKDGRTALHLCVESNHLESLKLLIETFM 130
            +    G TALH+       + +KLL+   M
Sbjct: 231 NIADTKGNTALHIAARKGRSQIVKLLLANNM 261

BLAST of CmoCh14G012810.1 vs. TAIR 10
Match: AT2G31820.1 (Ankyrin repeat family protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.4e-09
Identity = 31/79 (39.24%), Postives = 42/79 (53.16%), Query Frame = 0

Query: 39  TPLHLASKNGDIDTVRALLENNTSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSI 98
           T LH A+  G ID V  LLE +++   +  NNG   LH A   G VE+ K LI   P   
Sbjct: 260 TALHTAATQGHIDVVNLLLETDSNLAKIAKNNGKTALHSAARMGHVEVVKSLIGKDPSIG 319

Query: 99  WMKLKDGRTALHLCVESNH 118
           +   K G+TALH+ V+  +
Sbjct: 320 FRTDKKGQTALHMAVKGQN 338

BLAST of CmoCh14G012810.1 vs. TAIR 10
Match: AT5G54620.1 (Ankyrin repeat family protein )

HSP 1 Score: 59.7 bits (143), Expect = 3.1e-09
Identity = 34/101 (33.66%), Postives = 48/101 (47.52%), Query Frame = 0

Query: 39  TPLHLASKNGDIDTVRALLENNTSACLVYDNNGFIPLHYAVINGQVEITKELINATPQSI 98
           +PLHLA +N  +     L++ N    LV    G  PLH  V  G   +  E + A P+SI
Sbjct: 73  SPLHLAVENHQVQLALELVKINPDLVLVAGRKGMTPLHLVVKKGDANLLTEFLLACPESI 132

Query: 99  WMKLKDGRTALHLCVESNHLESLKLLIETFMNRHEVIATVT 140
                +G TALH+ V ++  E LK+L       H+  A  T
Sbjct: 133 KDTNVNGETALHIAVMNDRYEELKVLTGWIHRLHKSDAAST 173

BLAST of CmoCh14G012810.1 vs. TAIR 10
Match: AT5G54710.1 (Ankyrin repeat family protein )

HSP 1 Score: 55.5 bits (132), Expect = 5.9e-08
Identity = 34/101 (33.66%), Postives = 53/101 (52.48%), Query Frame = 0

Query: 9   TSSLNAIPDDDAHNQEIRIVMQSEVDAF----------QPTPLHLASKNGDIDTVRALL- 68
           TSS+  +  + A +++ ++++ S ++ F          Q T LH A K+G+++  R LL 
Sbjct: 159 TSSMTMVELNAAFSEQQQVIIDSILEKFPNLILDADEEQSTLLHKACKSGNLEMARTLLD 218

Query: 69  -ENNTSACLVYDNNGFIPLHYAVINGQVEITKELINATPQS 98
            + N       D +G  PLH AVING VEI KE +   P S
Sbjct: 219 VDVNQEIAEKVDKDGLTPLHRAVINGSVEILKEFLCKAPSS 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P161572.3e-0932.61Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3[more]
Q023575.2e-0933.33Ankyrin-1 OS=Mus musculus OX=10090 GN=Ank1 PE=1 SV=2[more]
Q182975.7e-0830.33Transient receptor potential cation channel subfamily A member 1 homolog OS=Caen... [more]
Q8N7Z58.3e-0734.41Ankyrin repeat domain-containing protein 31 OS=Homo sapiens OX=9606 GN=ANKRD31 P... [more]
P0DJE31.1e-0631.31Alpha-latrotoxin-Lhe1a OS=Latrodectus hesperus OX=256737 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1GU041.1e-9467.12ankyrin repeat-containing protein NPR4-like isoform X3 OS=Cucurbita moschata OX=... [more]
A0A6J1GTP74.9e-8761.97uncharacterized protein LOC111457477 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IU533.1e-8159.79ankyrin repeat-containing protein NPR4-like isoform X3 OS=Cucurbita maxima OX=36... [more]
A0A6J1IZU61.4e-7857.43ankyrin repeat-containing protein NPR4-like isoform X1 OS=Cucurbita maxima OX=36... [more]
A0A6J1IX061.4e-7857.43ankyrin repeat-containing protein NPR4-like isoform X2 OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
XP_022955458.12.3e-9467.12ankyrin repeat-containing protein NPR4-like isoform X3 [Cucurbita moschata][more]
XP_023528989.15.4e-8862.67ankyrin repeat-containing protein NPR4-like isoform X2 [Cucurbita pepo subsp. pe... [more]
XP_023528988.15.4e-8862.67ankyrin repeat-containing protein NPR4-like isoform X1 [Cucurbita pepo subsp. pe... [more]
XP_022955457.11.0e-8661.97uncharacterized protein LOC111457477 isoform X2 [Cucurbita moschata][more]
XP_022980641.16.4e-8159.79ankyrin repeat-containing protein NPR4-like isoform X3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G51160.11.3e-1032.32Ankyrin repeat family protein [more]
AT1G07710.11.4e-0936.26Ankyrin repeat family protein [more]
AT2G31820.12.4e-0939.24Ankyrin repeat family protein [more]
AT5G54620.13.1e-0933.66Ankyrin repeat family protein [more]
AT5G54710.15.9e-0833.66Ankyrin repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 36..65
e-value: 0.029
score: 23.5
coord: 70..102
e-value: 0.12
score: 21.5
coord: 104..133
e-value: 0.0015
score: 27.8
IPR002110Ankyrin repeatPFAMPF00023Ankcoord: 104..125
e-value: 0.0039
score: 17.6
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 36..68
score: 9.190411
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 104..126
score: 9.27054
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 70..92
score: 9.1637
IPR026961PGG domainPFAMPF13962PGGcoord: 134..196
e-value: 2.3E-9
score: 37.0
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 34..92
e-value: 7.0E-12
score: 45.8
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 18..161
e-value: 2.3E-23
score: 84.9
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 34..127
NoneNo IPR availablePANTHERPTHR24128HOMEOBOX PROTEIN WARIAIcoord: 39..130
coord: 134..196
NoneNo IPR availablePANTHERPTHR24128:SF61ANKYRIN REPEAT-CONTAINING PROTEIN ITN1-LIKEcoord: 39..130
coord: 134..196
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 36..62
score: 9.118069
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 104..126
score: 9.250726
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 70..92
score: 9.1446

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh14G012810CmoCh14G012810gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh14G012810.1:exon:6830CmoCh14G012810.1:exon:6830exon
CmoCh14G012810.1:exon:6829CmoCh14G012810.1:exon:6829exon
CmoCh14G012810.1:exon:6828CmoCh14G012810.1:exon:6828exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh14G012810.1:cdsCmoCh14G012810.1:cds_3CDS
CmoCh14G012810.1:cdsCmoCh14G012810.1:cds_2CDS
CmoCh14G012810.1:cdsCmoCh14G012810.1:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh14G012810.1CmoCh14G012810.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0005515 protein binding