CmoCh10G010850.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh10G010850.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGDSL esterase/lipase
LocationCmo_Chr10: 5945643 .. 5947623 (+)
Sequence length1376
RNA-Seq ExpressionCmoCh10G010850.1
SyntenyCmoCh10G010850.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCCATTTTTCACATCCAAATTCTTTCACTTCAACCTTTTTCCTTTTTCGTCTCGCCTTCAATGGCACCGCACTCGTTTGTCGACGTTCTTGTCATCGTGCTTGCCTTAGCTTCTTTGAGCCCCCGAGCCAAGGCGGGGAGGAGTTTCTTCGTCTTTGGAGACTCTCTCGTTGACAATGGCAATAACAACTACCTTGCCACCACAGCCCGTGCCGATGCATACCCGTATGGCATCGATTTCCCGACGCATCGCCCGACTGGCCGCTTCTCCAACGGCCTTAACATCCCTGATTACATAAGTTGGCATCATTTTCTCTCTCTGTTTTTAGTAGCTTTAATCTTTTATTCATATTCTTAACACACGACTTTCAGGTAAGCAACTCGGGTCGGAGTTCTTACTTCCTTATTTGAGTCCGGAGCTCAACAGTCGGAGGTTGCTCGACGGCGCCAATTTCGCTTCGGCTGGCATTGGCATTCTCAATGACACGGGAATTCAGTTTGTACGTCCACATTACTTCCTTCCTCTAATTCTTAAGTATCTTTTGACGATTTTATCGATGATGAATTCATCGACTCGCCCTTTGTGCCAATGTCGAAATGGTTGAAAATGTGCATGGTGCAGATCAATATAATAAGAATGTTCAGGCAATTAGAGTACTTTGAAGAGTACCAAAAGAGAGTTGCAGCCCTGATTGGAGCTCAAAAAACAACCCAACTCGTAAATGGAGCCTTGGTTCTGGTTACCGTTGGTGGAAATGATTTCGTCAATAATTACTATCTCGTCCCCTTCTCCGCTCGGTCAAGGCAATATGCTCTCCCCGATTACGTCAAGGTACTCATCGTTGAGTATCGAAAGCTCCTCCTGGTAATATCTCCCTTTCTCATTTTGTTCTATAAATTTTTTTGTTACGTCAAGGTACTCATCATTTAGTATAGATTAATTGAGGATGTATAAATTAAAACTTCAAACTAATAATTATATTAATTTAAACCCTAATTCTCGTACGTGGATAGAGTTAACCACTTTTTATTAATGATTTAATTTTTAACTAGTTTTACTTAATTAGATTTAAAATGACTTGAAAATAATAAAAATAAAATTACCCTAATTCTAAAATTCAAAATTAGAAAAATAATAATAATAAAGCATATACTCTCAACATCAACTCTCGATAAGACGATGAGATTGTAATTTTATGATGCAGAGGCTTCACGAGCTCGGAGCGCGACGAGTTCTGGTTACAGGAACAGGGCCGCTAGGGTGCGTCCCAGCAGAATTAGCGATGCGAGGAAGCAGCGGCGGGCAGTGCTCGGCGGAGCTGCAACGGGCAGCGGCATTGTATAATCCACAACTTCTTCAAATGATCAAAGGACTCAATGCCCAAGTTGGTTCCAATGTGTTTGTGGCTGTTAATACTCAACAAATGCACAATGATTTCGTCTCCAATCCTCAAGCATTTGGTATGTCTAAAATTTTGCTAAGTTTATGTCTATATGTCCTTTAATGATAATGTAAATTGGTGTATGGTAGGGTTTGAGACATCAAAAGTGGCATGTTGTGGACAAGGGCCATACAATGGCTTAGGGCTTTGCACCGAAGCATCAAACTTATGTTCGAACAGAGATGCGTATGCGTTTTGGGATGCCTTTCATCCTTCTCAAAAGGCTAATGGTATGATAGTGAAGCAAATGTTGTCGGGGTCAACCAAATATATGTACCCTATGAACCTCACAACCATCTTTGAATTGGATTCTAGGACTTGAAAAATTGCAATAAAAGCAATACCGAAAGCCACGAAACCAAGAAAGAACAAGACAGAGAAAAGATATAATCTTCTTTTGCCTCTTATAATCAAAAGGGTTTGAATTATGTCGTTTTTAATGTTGCATTTGCTTTGGTTTGTGTTTAGTGTTGTGTTGTTGGCTTGATCGAGAAAATGTGCATGTTTTGAATTATTTATATAATGACCTAACTTATAG

mRNA sequence

CCTCCATTTTTCACATCCAAATTCTTTCACTTCAACCTTTTTCCTTTTTCGTCTCGCCTTCAATGGCACCGCACTCGTTTGTCGACGTTCTTGTCATCGTGCTTGCCTTAGCTTCTTTGAGCCCCCGAGCCAAGGCGGGGAGGAGTTTCTTCGTCTTTGGAGACTCTCTCGTTGACAATGGCAATAACAACTACCTTGCCACCACAGCCCGTGCCGATGCATACCCGTATGGCATCGATTTCCCGACGCATCGCCCGACTGGCCGCTTCTCCAACGGCCTTAACATCCCTGATTACATAAGTAAGCAACTCGGGTCGGAGTTCTTACTTCCTTATTTGAGTCCGGAGCTCAACAGTCGGAGGTTGCTCGACGGCGCCAATTTCGCTTCGGCTGGCATTGGCATTCTCAATGACACGGGAATTCAGTTTATCAATATAATAAGAATGTTCAGGCAATTAGAGTACTTTGAAGAGTACCAAAAGAGAGTTGCAGCCCTGATTGGAGCTCAAAAAACAACCCAACTCGTAAATGGAGCCTTGGTTCTGGTTACCGTTGGTGGAAATGATTTCGTCAATAATTACTATCTCGTCCCCTTCTCCGCTCGGTCAAGGCAATATGCTCTCCCCGATTACGTCAAGGTACTCATCGTTGAGTATCGAAAGCTCCTCCTGAGGCTTCACGAGCTCGGAGCGCGACGAGTTCTGGTTACAGGAACAGGGCCGCTAGGGTGCGTCCCAGCAGAATTAGCGATGCGAGGAAGCAGCGGCGGGCAGTGCTCGGCGGAGCTGCAACGGGCAGCGGCATTGTATAATCCACAACTTCTTCAAATGATCAAAGGACTCAATGCCCAAGTTGGTTCCAATGTGTTTGTGGCTGTTAATACTCAACAAATGCACAATGATTTCGTCTCCAATCCTCAAGCATTTGGGTTTGAGACATCAAAAGTGGCATGTTGTGGACAAGGGCCATACAATGGCTTAGGGCTTTGCACCGAAGCATCAAACTTATGTTCGAACAGAGATGCGTATGCGTTTTGGGATGCCTTTCATCCTTCTCAAAAGGCTAATGGTATGATAGTGAAGCAAATGTTGTCGGGGTCAACCAAATATATGTACCCTATGAACCTCACAACCATCTTTGAATTGGATTCTAGGACTTGAAAAATTGCAATAAAAGCAATACCGAAAGCCACGAAACCAAGAAAGAACAAGACAGAGAAAAGATATAATCTTCTTTTGCCTCTTATAATCAAAAGGGTTTGAATTATGTCGTTTTTAATGTTGCATTTGCTTTGGTTTGTGTTTAGTGTTGTGTTGTTGGCTTGATCGAGAAAATGTGCATGTTTTGAATTATTTATATAATGACCTAACTTATAG

Coding sequence (CDS)

ATGGCACCGCACTCGTTTGTCGACGTTCTTGTCATCGTGCTTGCCTTAGCTTCTTTGAGCCCCCGAGCCAAGGCGGGGAGGAGTTTCTTCGTCTTTGGAGACTCTCTCGTTGACAATGGCAATAACAACTACCTTGCCACCACAGCCCGTGCCGATGCATACCCGTATGGCATCGATTTCCCGACGCATCGCCCGACTGGCCGCTTCTCCAACGGCCTTAACATCCCTGATTACATAAGTAAGCAACTCGGGTCGGAGTTCTTACTTCCTTATTTGAGTCCGGAGCTCAACAGTCGGAGGTTGCTCGACGGCGCCAATTTCGCTTCGGCTGGCATTGGCATTCTCAATGACACGGGAATTCAGTTTATCAATATAATAAGAATGTTCAGGCAATTAGAGTACTTTGAAGAGTACCAAAAGAGAGTTGCAGCCCTGATTGGAGCTCAAAAAACAACCCAACTCGTAAATGGAGCCTTGGTTCTGGTTACCGTTGGTGGAAATGATTTCGTCAATAATTACTATCTCGTCCCCTTCTCCGCTCGGTCAAGGCAATATGCTCTCCCCGATTACGTCAAGGTACTCATCGTTGAGTATCGAAAGCTCCTCCTGAGGCTTCACGAGCTCGGAGCGCGACGAGTTCTGGTTACAGGAACAGGGCCGCTAGGGTGCGTCCCAGCAGAATTAGCGATGCGAGGAAGCAGCGGCGGGCAGTGCTCGGCGGAGCTGCAACGGGCAGCGGCATTGTATAATCCACAACTTCTTCAAATGATCAAAGGACTCAATGCCCAAGTTGGTTCCAATGTGTTTGTGGCTGTTAATACTCAACAAATGCACAATGATTTCGTCTCCAATCCTCAAGCATTTGGGTTTGAGACATCAAAAGTGGCATGTTGTGGACAAGGGCCATACAATGGCTTAGGGCTTTGCACCGAAGCATCAAACTTATGTTCGAACAGAGATGCGTATGCGTTTTGGGATGCCTTTCATCCTTCTCAAAAGGCTAATGGTATGATAGTGAAGCAAATGTTGTCGGGGTCAACCAAATATATGTACCCTATGAACCTCACAACCATCTTTGAATTGGATTCTAGGACTTGA

Protein sequence

MAPHSFVDVLVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDFPTHRPTGRFSNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGIQFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSARSRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSAELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQGPYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFELDSRT
Homology
BLAST of CmoCh10G010850.1 vs. ExPASy Swiss-Prot
Match: Q8LB81 (GDSL esterase/lipase At5g33370 OS=Arabidopsis thaliana OX=3702 GN=At5g33370 PE=2 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 5.3e-145
Identity = 249/354 (70.34%), Postives = 302/354 (85.31%), Query Frame = 0

Query: 12  IVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDFPTHRPTGRFSN 71
           I+   + L P+A A R+F VFGDSLVDNGNN++LATTARAD YPYGIDFPTHRPTGRFSN
Sbjct: 15  ILQVTSLLVPQANA-RAFLVFGDSLVDNGNNDFLATTARADNYPYGIDFPTHRPTGRFSN 74

Query: 72  GLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGIQFINIIRMFRQ 131
           GLNIPD IS+ LG E  +PYLSP L   +LL GANFASAGIGILNDTGIQF+NIIR+ +Q
Sbjct: 75  GLNIPDLISEHLGQESPMPYLSPMLKKDKLLRGANFASAGIGILNDTGIQFLNIIRITKQ 134

Query: 132 LEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSARSRQYALPDYV 191
           LEYFE+Y+ RV+ L+G ++  +LVNGALVL+T+GGNDFVNNYYLVPFSARSRQ++LPDYV
Sbjct: 135 LEYFEQYKVRVSGLVGEEEMNRLVNGALVLITLGGNDFVNNYYLVPFSARSRQFSLPDYV 194

Query: 192 KVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSAELQRAAALYNP 251
             +I EYRK+L ++++LGARRVLVTGTGP+GCVPAELA R S  G+C+ ELQRAA+L+NP
Sbjct: 195 VFVISEYRKVLRKMYDLGARRVLVTGTGPMGCVPAELAQR-SRNGECATELQRAASLFNP 254

Query: 252 QLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQGPYNGLGLCTE 311
           QL+QMI  LN +VGS+ F+A NTQQMH DF+S+PQA+GF TSKVACCGQGPYNG+GLCT 
Sbjct: 255 QLIQMITDLNNEVGSSAFIAANTQQMHMDFISDPQAYGFVTSKVACCGQGPYNGIGLCTP 314

Query: 312 ASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFELDSRT 366
            SNLC NRD +AFWD FHPS+KA+ +I +Q+L+GS +YM+PMNL+TI  +DS T
Sbjct: 315 LSNLCPNRDLFAFWDPFHPSEKASRIIAQQILNGSPEYMHPMNLSTILTVDSMT 366

BLAST of CmoCh10G010850.1 vs. ExPASy Swiss-Prot
Match: Q5PNZ0 (GDSL esterase/lipase At5g18430 OS=Arabidopsis thaliana OX=3702 GN=At5g18430 PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 7.9e-141
Identity = 244/362 (67.40%), Postives = 294/362 (81.22%), Query Frame = 0

Query: 1   MAPHSFVDVLVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDF 60
           M   + +  + + L      P    GR+FFVFGDSLVD+GNNNYL TTARAD+ PYGIDF
Sbjct: 1   MTISTVIAFMSMFLVFVMSGPIVVEGRAFFVFGDSLVDSGNNNYLVTTARADSPPYGIDF 60

Query: 61  PTHRPTGRFSNGLNIPDYISKQLGSEF-LLPYLSPELNSRRLLDGANFASAGIGILNDTG 120
           PT RPTGRFSNGLNIPD IS+ +G+E   LPYLSPEL  R LL+GANFASAGIGILNDTG
Sbjct: 61  PTRRPTGRFSNGLNIPDLISEAIGNEEPPLPYLSPELRGRSLLNGANFASAGIGILNDTG 120

Query: 121 IQFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFS 180
            QFINIIRM++QL+YF++YQ+RV+ LIG  +T +LV+ ALVL+TVGGNDFVNNY+L P+S
Sbjct: 121 FQFINIIRMYQQLDYFQQYQQRVSRLIGKPQTQRLVSQALVLITVGGNDFVNNYFLFPYS 180

Query: 181 ARSRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCS 240
           ARSRQ+ LPDYV++LI EY+K+LLRL+ LG  RVLVTG GPLGC PAELA  G+S G+CS
Sbjct: 181 ARSRQFTLPDYVRLLISEYKKILLRLNSLGVGRVLVTGAGPLGCAPAELARSGTSNGRCS 240

Query: 241 AELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCG 300
           AELQRAA+LY+PQLLQMI  LN ++G NVF+A NT QM  DF+S P+ +GF TSKVACCG
Sbjct: 241 AELQRAASLYDPQLLQMINELNKKIGRNVFIAANTNQMQEDFLSTPRRYGFVTSKVACCG 300

Query: 301 QGPYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIF 360
           QGPYNG+GLCT  SNLC NR+ Y FWDAFHP++KAN MIV+ +L+G+TKYM PMNL++  
Sbjct: 301 QGPYNGMGLCTVLSNLCPNRELYVFWDAFHPTEKANRMIVRHILTGTTKYMNPMNLSSAL 360

Query: 361 EL 362
            L
Sbjct: 361 AL 362

BLAST of CmoCh10G010850.1 vs. ExPASy Swiss-Prot
Match: Q9M8Y5 (GDSL esterase/lipase LTL1 OS=Arabidopsis thaliana OX=3702 GN=LTL1 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.4e-140
Identity = 247/362 (68.23%), Postives = 294/362 (81.22%), Query Frame = 0

Query: 2   APHSFVDVLVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDFP 61
           +P  F+  L  ++    L+P+ K+ R+FFVFGDSLVDNGNN+YL TTARAD YPYGID+P
Sbjct: 6   SPLGFLISLFFIVTF--LAPQVKS-RAFFVFGDSLVDNGNNDYLVTTARADNYPYGIDYP 65

Query: 62  THRPTGRFSNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGIQ 121
           T RPTGRFSNGLNIPD IS+ +G    LPYLSP L    LL GANFASAGIGILNDTGIQ
Sbjct: 66  TRRPTGRFSNGLNIPDIISEAIGMPSTLPYLSPHLTGENLLVGANFASAGIGILNDTGIQ 125

Query: 122 FINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSAR 181
           F+NIIR+ +Q+EYFE+YQ RV+ALIG + T QLVN ALVL+T+GGNDFVNNYYL+PFSAR
Sbjct: 126 FVNIIRISKQMEYFEQYQLRVSALIGPEATQQLVNQALVLITLGGNDFVNNYYLIPFSAR 185

Query: 182 SRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSAE 241
           SRQYALPDYV  LI EY K+L +L+ELGARRVLVTGTG +GC PAELA   S  G+C   
Sbjct: 186 SRQYALPDYVVYLISEYGKILRKLYELGARRVLVTGTGAMGCAPAELAQH-SRNGECYGA 245

Query: 242 LQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQG 301
           LQ AAAL+NPQL+ +I  +NA++G +VFVA N  QM+ D++SNP+ FGF TSKVACCGQG
Sbjct: 246 LQTAAALFNPQLVDLIASVNAEIGQDVFVAANAYQMNMDYLSNPEQFGFVTSKVACCGQG 305

Query: 302 PYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFEL 361
           PYNG+GLCT  SNLC NRD YAFWDAFHP++KAN +IV Q+L+GS+KYM+PMNL+T   L
Sbjct: 306 PYNGIGLCTPVSNLCPNRDLYAFWDAFHPTEKANRIIVNQILTGSSKYMHPMNLSTAMLL 363

Query: 362 DS 364
           DS
Sbjct: 366 DS 363

BLAST of CmoCh10G010850.1 vs. ExPASy Swiss-Prot
Match: Q9SVU5 (GDSL esterase/lipase At4g28780 OS=Arabidopsis thaliana OX=3702 GN=At4g28780 PE=2 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 4.0e-132
Identity = 227/355 (63.94%), Postives = 288/355 (81.13%), Query Frame = 0

Query: 10  LVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDFPTHRPTGRF 69
           + + + L  +  +  A R+FFVFGDSLVD+GNNNYL TTARAD+ PYGID+PT RPTGRF
Sbjct: 13  VALSVTLFLMPQQTNAARAFFVFGDSLVDSGNNNYLVTTARADSPPYGIDYPTGRPTGRF 72

Query: 70  SNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGIQFINIIRMF 129
           SNGLN+PD IS+Q+GSE  LP LSPEL   +LL GANFASAGIGILNDTG+QF+NI+R+ 
Sbjct: 73  SNGLNLPDIISEQIGSEPTLPILSPELTGEKLLIGANFASAGIGILNDTGVQFLNILRIG 132

Query: 130 RQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSARSRQYALPD 189
           RQ E F+EYQ+RV+ +IG+ KT QLVNGALVL+T+GGNDFVNNY+  P S R RQ +L +
Sbjct: 133 RQFELFQEYQERVSEIIGSDKTQQLVNGALVLMTLGGNDFVNNYFF-PISTRRRQSSLGE 192

Query: 190 YVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSAELQRAAALY 249
           + ++LI EY+K+L  L+ELGARRV+VTGTGPLGCVPAELA  GS  G+C+ E Q+AAA++
Sbjct: 193 FSQLLISEYKKILTSLYELGARRVMVTGTGPLGCVPAELASSGSVNGECAPEAQQAAAIF 252

Query: 250 NPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQGPYNGLGLC 309
           NP L+QM++GLN ++GS+VF+  N    + DF++NPQ FGF TSKVACCGQG YNG G+C
Sbjct: 253 NPLLVQMLQGLNREIGSDVFIGANAFNTNADFINNPQRFGFVTSKVACCGQGAYNGQGVC 312

Query: 310 TEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFELDSR 365
           T  S LCS+R+AYAFWD FHP++KA  +IV+Q+++GS +YM PMNL+TI  LDSR
Sbjct: 313 TPLSTLCSDRNAYAFWDPFHPTEKATRLIVQQIMTGSVEYMNPMNLSTIMALDSR 366

BLAST of CmoCh10G010850.1 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 254.2 bits (648), Expect = 2.2e-66
Identity = 139/357 (38.94%), Postives = 211/357 (59.10%), Query Frame = 0

Query: 11  VIVLALASLSPRAKAGRSF---FVFGDSLVDNGNNNYLATTARADAYPYGIDFPTHRPTG 70
           +IVL++   S    AG+     FVFGDSLVD GNNNYLAT ++A+  P GIDF +  PTG
Sbjct: 10  IIVLSVLFFSEVCLAGKKIPANFVFGDSLVDAGNNNYLATLSKANYVPNGIDFGS--PTG 69

Query: 71  RFSNGLNIPDYISKQLGSEFLL-PYLSPELNSRRLLDGANFASAGIGILNDTGIQFINII 130
           RF+NG  I D + + LGS+ L  PYL+P  +   +L+G N+AS G GILN TG  F   I
Sbjct: 70  RFTNGRTIVDIVYQALGSDELTPPYLAPTTSGSLILNGVNYASGGSGILNSTGKLFGERI 129

Query: 131 RMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSARSRQYA 190
            +  QL+ F   ++ + + IG  +  +L   A+  VT G ND +NNY+    S   R+  
Sbjct: 130 NVDAQLDNFATTRQDIISWIGESEAAKLFRSAIFSVTTGSNDLINNYFTPVISTLQRKVV 189

Query: 191 LPD-YVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSAELQRA 250
            P+ +V  +I ++R  L RL++LGAR+++V   GP+GC+P E     ++G  C AE    
Sbjct: 190 APEVFVDTMISKFRLQLTRLYQLGARKIVVINIGPIGCIPFERESDPAAGNNCLAEPNEV 249

Query: 251 AALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCG-QGPYN 310
           A +YN +L  +++ LN  +  + FV  +  ++ +D + N  ++GFE+ K+ CC   G   
Sbjct: 250 AQMYNLKLKTLVEELNKNLQGSRFVYGDVFRIVDDIIQNYSSYGFESEKIPCCSLVGKVG 309

Query: 311 GLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFEL 362
           GL  C   S +C +R  Y FWD +HP++ AN +I +++LSG T  +YP+N+  +  L
Sbjct: 310 GLIPCGPPSKVCMDRSKYVFWDPYHPTEAANIIIARRLLSGDTSDIYPINIRQLANL 364

BLAST of CmoCh10G010850.1 vs. ExPASy TrEMBL
Match: A0A6J1HBF2 (GDSL esterase/lipase At5g33370-like OS=Cucurbita moschata OX=3662 GN=LOC111461320 PE=3 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.1e-206
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 1   MAPHSFVDVLVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDF 60
           MAPHSFVDVLVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDF
Sbjct: 1   MAPHSFVDVLVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDF 60

Query: 61  PTHRPTGRFSNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGI 120
           PTHRPTGRFSNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGI
Sbjct: 61  PTHRPTGRFSNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGI 120

Query: 121 QFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSA 180
           QFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSA
Sbjct: 121 QFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSA 180

Query: 181 RSRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSA 240
           RSRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSA
Sbjct: 181 RSRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSA 240

Query: 241 ELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQ 300
           ELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQ
Sbjct: 241 ELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQ 300

Query: 301 GPYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFE 360
           GPYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFE
Sbjct: 301 GPYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFE 360

Query: 361 LDSRT 366
           LDSRT
Sbjct: 361 LDSRT 365

BLAST of CmoCh10G010850.1 vs. ExPASy TrEMBL
Match: A0A6J1JLG1 (GDSL esterase/lipase At5g33370-like OS=Cucurbita maxima OX=3661 GN=LOC111485480 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 9.3e-201
Identity = 353/364 (96.98%), Postives = 360/364 (98.90%), Query Frame = 0

Query: 1   MAPHSFVDVLVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDF 60
           MAP SF+DVLV+VLALA+LS RAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDF
Sbjct: 1   MAPRSFLDVLVLVLALAALSHRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDF 60

Query: 61  PTHRPTGRFSNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGI 120
           PTHRPTGRFSNGLNIPDY+SKQLGSEFLLPYLSPELN RRLLDGANFASAGIGILNDTGI
Sbjct: 61  PTHRPTGRFSNGLNIPDYLSKQLGSEFLLPYLSPELNGRRLLDGANFASAGIGILNDTGI 120

Query: 121 QFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSA 180
           QFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSA
Sbjct: 121 QFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSA 180

Query: 181 RSRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSA 240
           RSRQYALPDYVKVLIVEY+KLL+RLHELGARRVLVTGTGPLGCVPAELAMRGS+GGQCSA
Sbjct: 181 RSRQYALPDYVKVLIVEYQKLLMRLHELGARRVLVTGTGPLGCVPAELAMRGSNGGQCSA 240

Query: 241 ELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQ 300
           ELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQ
Sbjct: 241 ELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQ 300

Query: 301 GPYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFE 360
           GPYNGLGLCT ASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFE
Sbjct: 301 GPYNGLGLCTGASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFE 360

Query: 361 LDSR 365
           LDSR
Sbjct: 361 LDSR 364

BLAST of CmoCh10G010850.1 vs. ExPASy TrEMBL
Match: A0A5A7T4K1 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G003110 PE=3 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 4.0e-180
Identity = 313/366 (85.52%), Postives = 345/366 (94.26%), Query Frame = 0

Query: 1   MAPHSFVDVLVIVLA-LASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGID 60
           MAP   V +L +VLA LA+L+ +A+AGR+FFVFGDSLVDNGNNNYLATTARAD+YPYGID
Sbjct: 1   MAPRLSVAILGVVLAILAALTAQAEAGRAFFVFGDSLVDNGNNNYLATTARADSYPYGID 60

Query: 61  FPTHRPTGRFSNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTG 120
           FPTHRPTGRFSNGLNIPDYIS+QLGSEFLLPYL+PELN RRLLDGANFASAGIGILNDTG
Sbjct: 61  FPTHRPTGRFSNGLNIPDYISQQLGSEFLLPYLNPELNGRRLLDGANFASAGIGILNDTG 120

Query: 121 IQFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFS 180
           IQFINIIRMFRQ EYFEEYQ+RV  +IG ++  +LV GALVL+TVGGNDFVNNYYLVPFS
Sbjct: 121 IQFINIIRMFRQFEYFEEYQRRVGRVIGDERAKELVKGALVLITVGGNDFVNNYYLVPFS 180

Query: 181 ARSRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCS 240
           ARSRQY+LPDYV +LIVEYRK+LLRL+ELGARRVLVTGTGPLGCVPAELAMRGS+GGQCS
Sbjct: 181 ARSRQYSLPDYVNLLIVEYRKILLRLYELGARRVLVTGTGPLGCVPAELAMRGSTGGQCS 240

Query: 241 AELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCG 300
           AELQRAAALYNP+L QMIKGLN+Q+GSNVFVAVNTQQMH DF+SNP+A+GFETSKVACCG
Sbjct: 241 AELQRAAALYNPKLFQMIKGLNSQLGSNVFVAVNTQQMHIDFISNPRAYGFETSKVACCG 300

Query: 301 QGPYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIF 360
           QGPYNGLGLCT ASNLCSNRDAYAFWDAFHPSQKANG+IVKQM SG+T+YMYPMNLTTI 
Sbjct: 301 QGPYNGLGLCTVASNLCSNRDAYAFWDAFHPSQKANGIIVKQMFSGTTQYMYPMNLTTIL 360

Query: 361 ELDSRT 366
           +LDS+T
Sbjct: 361 QLDSKT 366

BLAST of CmoCh10G010850.1 vs. ExPASy TrEMBL
Match: A0A1S3B8S0 (GDSL esterase/lipase At5g33370 OS=Cucumis melo OX=3656 GN=LOC103487277 PE=3 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 4.0e-180
Identity = 313/366 (85.52%), Postives = 345/366 (94.26%), Query Frame = 0

Query: 1   MAPHSFVDVLVIVLA-LASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGID 60
           MAP   V +L +VLA LA+L+ +A+AGR+FFVFGDSLVDNGNNNYLATTARAD+YPYGID
Sbjct: 1   MAPRLSVAILGVVLAILAALTAQAEAGRAFFVFGDSLVDNGNNNYLATTARADSYPYGID 60

Query: 61  FPTHRPTGRFSNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTG 120
           FPTHRPTGRFSNGLNIPDYIS+QLGSEFLLPYL+PELN RRLLDGANFASAGIGILNDTG
Sbjct: 61  FPTHRPTGRFSNGLNIPDYISQQLGSEFLLPYLNPELNGRRLLDGANFASAGIGILNDTG 120

Query: 121 IQFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFS 180
           IQFINIIRMFRQ EYFEEYQ+RV  +IG ++  +LV GALVL+TVGGNDFVNNYYLVPFS
Sbjct: 121 IQFINIIRMFRQFEYFEEYQRRVGRVIGDERAKELVKGALVLITVGGNDFVNNYYLVPFS 180

Query: 181 ARSRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCS 240
           ARSRQY+LPDYV +LIVEYRK+LLRL+ELGARRVLVTGTGPLGCVPAELAMRGS+GGQCS
Sbjct: 181 ARSRQYSLPDYVNLLIVEYRKILLRLYELGARRVLVTGTGPLGCVPAELAMRGSTGGQCS 240

Query: 241 AELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCG 300
           AELQRAAALYNP+L QMIKGLN+Q+GSNVFVAVNTQQMH DF+SNP+A+GFETSKVACCG
Sbjct: 241 AELQRAAALYNPKLFQMIKGLNSQLGSNVFVAVNTQQMHIDFISNPRAYGFETSKVACCG 300

Query: 301 QGPYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIF 360
           QGPYNGLGLCT ASNLCSNRDAYAFWDAFHPSQKANG+IVKQM SG+T+YMYPMNLTTI 
Sbjct: 301 QGPYNGLGLCTVASNLCSNRDAYAFWDAFHPSQKANGIIVKQMFSGTTQYMYPMNLTTIL 360

Query: 361 ELDSRT 366
           +LDS+T
Sbjct: 361 QLDSKT 366

BLAST of CmoCh10G010850.1 vs. ExPASy TrEMBL
Match: A0A6J1DBW4 (GDSL esterase/lipase At5g33370-like OS=Momordica charantia OX=3673 GN=LOC111019605 PE=3 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 3.2e-177
Identity = 306/365 (83.84%), Postives = 342/365 (93.70%), Query Frame = 0

Query: 1   MAPHSFVDVLVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDF 60
           MA  S++ VL +VLAL +LS +A+AGR+FFVFGDSLVDNGNNNYLATTARAD+ PYGIDF
Sbjct: 1   MAARSWL-VLGLVLALGALSSQAEAGRAFFVFGDSLVDNGNNNYLATTARADSLPYGIDF 60

Query: 61  PTHRPTGRFSNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGI 120
           PTHRPTGRFSNGLNIPD+I K LGS+FLLPYLSPELN R+LLDGANFASAGIGILNDTGI
Sbjct: 61  PTHRPTGRFSNGLNIPDFICKHLGSDFLLPYLSPELNGRKLLDGANFASAGIGILNDTGI 120

Query: 121 QFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSA 180
           QFIN+IRMFRQLEYFEEYQKRV  +IGA++TTQLVNGALV++TVGGNDFVNNY+LVPFSA
Sbjct: 121 QFINVIRMFRQLEYFEEYQKRVGGIIGAERTTQLVNGALVIITVGGNDFVNNYFLVPFSA 180

Query: 181 RSRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSA 240
           RSRQYALPDYVK+LIVEYRKLLLR++ELGARRVLVTGTGPLGCVPAELAMRG +GGQCS 
Sbjct: 181 RSRQYALPDYVKLLIVEYRKLLLRVYELGARRVLVTGTGPLGCVPAELAMRGGNGGQCST 240

Query: 241 ELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQ 300
           ELQRAA+LYNPQLL+MIKGLN+Q+GSNVFVAVNT  MHNDF++NP  FGF+TS+VACCGQ
Sbjct: 241 ELQRAASLYNPQLLEMIKGLNSQIGSNVFVAVNTHLMHNDFITNPLPFGFQTSRVACCGQ 300

Query: 301 GPYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFE 360
           GP+NGLGLCT ASNLCSNRD YAFWDAFHPSQKANG+IVKQ+  G+T+YMYPMNL+TI E
Sbjct: 301 GPFNGLGLCTVASNLCSNRDDYAFWDAFHPSQKANGIIVKQIFQGTTQYMYPMNLSTILE 360

Query: 361 LDSRT 366
           LDSRT
Sbjct: 361 LDSRT 364

BLAST of CmoCh10G010850.1 vs. TAIR 10
Match: AT5G33370.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 515.4 bits (1326), Expect = 3.8e-146
Identity = 249/354 (70.34%), Postives = 302/354 (85.31%), Query Frame = 0

Query: 12  IVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDFPTHRPTGRFSN 71
           I+   + L P+A A R+F VFGDSLVDNGNN++LATTARAD YPYGIDFPTHRPTGRFSN
Sbjct: 15  ILQVTSLLVPQANA-RAFLVFGDSLVDNGNNDFLATTARADNYPYGIDFPTHRPTGRFSN 74

Query: 72  GLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGIQFINIIRMFRQ 131
           GLNIPD IS+ LG E  +PYLSP L   +LL GANFASAGIGILNDTGIQF+NIIR+ +Q
Sbjct: 75  GLNIPDLISEHLGQESPMPYLSPMLKKDKLLRGANFASAGIGILNDTGIQFLNIIRITKQ 134

Query: 132 LEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSARSRQYALPDYV 191
           LEYFE+Y+ RV+ L+G ++  +LVNGALVL+T+GGNDFVNNYYLVPFSARSRQ++LPDYV
Sbjct: 135 LEYFEQYKVRVSGLVGEEEMNRLVNGALVLITLGGNDFVNNYYLVPFSARSRQFSLPDYV 194

Query: 192 KVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSAELQRAAALYNP 251
             +I EYRK+L ++++LGARRVLVTGTGP+GCVPAELA R S  G+C+ ELQRAA+L+NP
Sbjct: 195 VFVISEYRKVLRKMYDLGARRVLVTGTGPMGCVPAELAQR-SRNGECATELQRAASLFNP 254

Query: 252 QLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQGPYNGLGLCTE 311
           QL+QMI  LN +VGS+ F+A NTQQMH DF+S+PQA+GF TSKVACCGQGPYNG+GLCT 
Sbjct: 255 QLIQMITDLNNEVGSSAFIAANTQQMHMDFISDPQAYGFVTSKVACCGQGPYNGIGLCTP 314

Query: 312 ASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFELDSRT 366
            SNLC NRD +AFWD FHPS+KA+ +I +Q+L+GS +YM+PMNL+TI  +DS T
Sbjct: 315 LSNLCPNRDLFAFWDPFHPSEKASRIIAQQILNGSPEYMHPMNLSTILTVDSMT 366

BLAST of CmoCh10G010850.1 vs. TAIR 10
Match: AT5G18430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 501.5 bits (1290), Expect = 5.6e-142
Identity = 244/362 (67.40%), Postives = 294/362 (81.22%), Query Frame = 0

Query: 1   MAPHSFVDVLVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDF 60
           M   + +  + + L      P    GR+FFVFGDSLVD+GNNNYL TTARAD+ PYGIDF
Sbjct: 1   MTISTVIAFMSMFLVFVMSGPIVVEGRAFFVFGDSLVDSGNNNYLVTTARADSPPYGIDF 60

Query: 61  PTHRPTGRFSNGLNIPDYISKQLGSEF-LLPYLSPELNSRRLLDGANFASAGIGILNDTG 120
           PT RPTGRFSNGLNIPD IS+ +G+E   LPYLSPEL  R LL+GANFASAGIGILNDTG
Sbjct: 61  PTRRPTGRFSNGLNIPDLISEAIGNEEPPLPYLSPELRGRSLLNGANFASAGIGILNDTG 120

Query: 121 IQFINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFS 180
            QFINIIRM++QL+YF++YQ+RV+ LIG  +T +LV+ ALVL+TVGGNDFVNNY+L P+S
Sbjct: 121 FQFINIIRMYQQLDYFQQYQQRVSRLIGKPQTQRLVSQALVLITVGGNDFVNNYFLFPYS 180

Query: 181 ARSRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCS 240
           ARSRQ+ LPDYV++LI EY+K+LLRL+ LG  RVLVTG GPLGC PAELA  G+S G+CS
Sbjct: 181 ARSRQFTLPDYVRLLISEYKKILLRLNSLGVGRVLVTGAGPLGCAPAELARSGTSNGRCS 240

Query: 241 AELQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCG 300
           AELQRAA+LY+PQLLQMI  LN ++G NVF+A NT QM  DF+S P+ +GF TSKVACCG
Sbjct: 241 AELQRAASLYDPQLLQMINELNKKIGRNVFIAANTNQMQEDFLSTPRRYGFVTSKVACCG 300

Query: 301 QGPYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIF 360
           QGPYNG+GLCT  SNLC NR+ Y FWDAFHP++KAN MIV+ +L+G+TKYM PMNL++  
Sbjct: 301 QGPYNGMGLCTVLSNLCPNRELYVFWDAFHPTEKANRMIVRHILTGTTKYMNPMNLSSAL 360

Query: 361 EL 362
            L
Sbjct: 361 AL 362

BLAST of CmoCh10G010850.1 vs. TAIR 10
Match: AT3G04290.1 (Li-tolerant lipase 1 )

HSP 1 Score: 500.7 bits (1288), Expect = 9.6e-142
Identity = 247/362 (68.23%), Postives = 294/362 (81.22%), Query Frame = 0

Query: 2   APHSFVDVLVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDFP 61
           +P  F+  L  ++    L+P+ K+ R+FFVFGDSLVDNGNN+YL TTARAD YPYGID+P
Sbjct: 6   SPLGFLISLFFIVTF--LAPQVKS-RAFFVFGDSLVDNGNNDYLVTTARADNYPYGIDYP 65

Query: 62  THRPTGRFSNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGIQ 121
           T RPTGRFSNGLNIPD IS+ +G    LPYLSP L    LL GANFASAGIGILNDTGIQ
Sbjct: 66  TRRPTGRFSNGLNIPDIISEAIGMPSTLPYLSPHLTGENLLVGANFASAGIGILNDTGIQ 125

Query: 122 FINIIRMFRQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSAR 181
           F+NIIR+ +Q+EYFE+YQ RV+ALIG + T QLVN ALVL+T+GGNDFVNNYYL+PFSAR
Sbjct: 126 FVNIIRISKQMEYFEQYQLRVSALIGPEATQQLVNQALVLITLGGNDFVNNYYLIPFSAR 185

Query: 182 SRQYALPDYVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSAE 241
           SRQYALPDYV  LI EY K+L +L+ELGARRVLVTGTG +GC PAELA   S  G+C   
Sbjct: 186 SRQYALPDYVVYLISEYGKILRKLYELGARRVLVTGTGAMGCAPAELAQH-SRNGECYGA 245

Query: 242 LQRAAALYNPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQG 301
           LQ AAAL+NPQL+ +I  +NA++G +VFVA N  QM+ D++SNP+ FGF TSKVACCGQG
Sbjct: 246 LQTAAALFNPQLVDLIASVNAEIGQDVFVAANAYQMNMDYLSNPEQFGFVTSKVACCGQG 305

Query: 302 PYNGLGLCTEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFEL 361
           PYNG+GLCT  SNLC NRD YAFWDAFHP++KAN +IV Q+L+GS+KYM+PMNL+T   L
Sbjct: 306 PYNGIGLCTPVSNLCPNRDLYAFWDAFHPTEKANRIIVNQILTGSSKYMHPMNLSTAMLL 363

Query: 362 DS 364
           DS
Sbjct: 366 DS 363

BLAST of CmoCh10G010850.1 vs. TAIR 10
Match: AT4G28780.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 472.6 bits (1215), Expect = 2.8e-133
Identity = 227/355 (63.94%), Postives = 288/355 (81.13%), Query Frame = 0

Query: 10  LVIVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDFPTHRPTGRF 69
           + + + L  +  +  A R+FFVFGDSLVD+GNNNYL TTARAD+ PYGID+PT RPTGRF
Sbjct: 13  VALSVTLFLMPQQTNAARAFFVFGDSLVDSGNNNYLVTTARADSPPYGIDYPTGRPTGRF 72

Query: 70  SNGLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGIQFINIIRMF 129
           SNGLN+PD IS+Q+GSE  LP LSPEL   +LL GANFASAGIGILNDTG+QF+NI+R+ 
Sbjct: 73  SNGLNLPDIISEQIGSEPTLPILSPELTGEKLLIGANFASAGIGILNDTGVQFLNILRIG 132

Query: 130 RQLEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSARSRQYALPD 189
           RQ E F+EYQ+RV+ +IG+ KT QLVNGALVL+T+GGNDFVNNY+  P S R RQ +L +
Sbjct: 133 RQFELFQEYQERVSEIIGSDKTQQLVNGALVLMTLGGNDFVNNYFF-PISTRRRQSSLGE 192

Query: 190 YVKVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSAELQRAAALY 249
           + ++LI EY+K+L  L+ELGARRV+VTGTGPLGCVPAELA  GS  G+C+ E Q+AAA++
Sbjct: 193 FSQLLISEYKKILTSLYELGARRVMVTGTGPLGCVPAELASSGSVNGECAPEAQQAAAIF 252

Query: 250 NPQLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFGFETSKVACCGQGPYNGLGLC 309
           NP L+QM++GLN ++GS+VF+  N    + DF++NPQ FGF TSKVACCGQG YNG G+C
Sbjct: 253 NPLLVQMLQGLNREIGSDVFIGANAFNTNADFINNPQRFGFVTSKVACCGQGAYNGQGVC 312

Query: 310 TEASNLCSNRDAYAFWDAFHPSQKANGMIVKQMLSGSTKYMYPMNLTTIFELDSR 365
           T  S LCS+R+AYAFWD FHP++KA  +IV+Q+++GS +YM PMNL+TI  LDSR
Sbjct: 313 TPLSTLCSDRNAYAFWDPFHPTEKATRLIVQQIMTGSVEYMNPMNLSTIMALDSR 366

BLAST of CmoCh10G010850.1 vs. TAIR 10
Match: AT5G33370.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 399.4 bits (1025), Expect = 3.0e-111
Identity = 197/278 (70.86%), Postives = 238/278 (85.61%), Query Frame = 0

Query: 12  IVLALASLSPRAKAGRSFFVFGDSLVDNGNNNYLATTARADAYPYGIDFPTHRPTGRFSN 71
           I+   + L P+A A R+F VFGDSLVDNGNN++LATTARAD YPYGIDFPTHRPTGRFSN
Sbjct: 15  ILQVTSLLVPQANA-RAFLVFGDSLVDNGNNDFLATTARADNYPYGIDFPTHRPTGRFSN 74

Query: 72  GLNIPDYISKQLGSEFLLPYLSPELNSRRLLDGANFASAGIGILNDTGIQFINIIRMFRQ 131
           GLNIPD IS+ LG E  +PYLSP L   +LL GANFASAGIGILNDTGIQF+NIIR+ +Q
Sbjct: 75  GLNIPDLISEHLGQESPMPYLSPMLKKDKLLRGANFASAGIGILNDTGIQFLNIIRITKQ 134

Query: 132 LEYFEEYQKRVAALIGAQKTTQLVNGALVLVTVGGNDFVNNYYLVPFSARSRQYALPDYV 191
           LEYFE+Y+ RV+ L+G ++  +LVNGALVL+T+GGNDFVNNYYLVPFSARSRQ++LPDYV
Sbjct: 135 LEYFEQYKVRVSGLVGEEEMNRLVNGALVLITLGGNDFVNNYYLVPFSARSRQFSLPDYV 194

Query: 192 KVLIVEYRKLLLRLHELGARRVLVTGTGPLGCVPAELAMRGSSGGQCSAELQRAAALYNP 251
             +I EYRK+L ++++LGARRVLVTGTGP+GCVPAELA R S  G+C+ ELQRAA+L+NP
Sbjct: 195 VFVISEYRKVLRKMYDLGARRVLVTGTGPMGCVPAELAQR-SRNGECATELQRAASLFNP 254

Query: 252 QLLQMIKGLNAQVGSNVFVAVNTQQMHNDFVSNPQAFG 290
           QL+QMI  LN +VGS+ F+A NTQQMH DF+S+PQA+G
Sbjct: 255 QLIQMITDLNNEVGSSAFIAANTQQMHMDFISDPQAYG 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LB815.3e-14570.34GDSL esterase/lipase At5g33370 OS=Arabidopsis thaliana OX=3702 GN=At5g33370 PE=2... [more]
Q5PNZ07.9e-14167.40GDSL esterase/lipase At5g18430 OS=Arabidopsis thaliana OX=3702 GN=At5g18430 PE=2... [more]
Q9M8Y51.4e-14068.23GDSL esterase/lipase LTL1 OS=Arabidopsis thaliana OX=3702 GN=LTL1 PE=2 SV=1[more]
Q9SVU54.0e-13263.94GDSL esterase/lipase At4g28780 OS=Arabidopsis thaliana OX=3702 GN=At4g28780 PE=2... [more]
O234702.2e-6638.94GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3... [more]
Match NameE-valueIdentityDescription
A0A6J1HBF21.1e-206100.00GDSL esterase/lipase At5g33370-like OS=Cucurbita moschata OX=3662 GN=LOC11146132... [more]
A0A6J1JLG19.3e-20196.98GDSL esterase/lipase At5g33370-like OS=Cucurbita maxima OX=3661 GN=LOC111485480 ... [more]
A0A5A7T4K14.0e-18085.52GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270... [more]
A0A1S3B8S04.0e-18085.52GDSL esterase/lipase At5g33370 OS=Cucumis melo OX=3656 GN=LOC103487277 PE=3 SV=1[more]
A0A6J1DBW43.2e-17783.84GDSL esterase/lipase At5g33370-like OS=Momordica charantia OX=3673 GN=LOC1110196... [more]
Match NameE-valueIdentityDescription
AT5G33370.13.8e-14670.34GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G18430.15.6e-14267.40GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G04290.19.6e-14268.23Li-tolerant lipase 1 [more]
AT4G28780.12.8e-13363.94GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G33370.23.0e-11170.86GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 30..340
e-value: 1.0E-25
score: 91.5
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 21..348
e-value: 4.6E-74
score: 251.7
NoneNo IPR availablePANTHERPTHR45648GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)coord: 10..361
NoneNo IPR availablePANTHERPTHR45648:SF83FINGER PROTEIN, PUTATIVE-RELATEDcoord: 10..361
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 28..345
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 28..346
e-value: 3.12755E-128
score: 368.481

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh10G010850CmoCh10G010850gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G010850.1:exon:8273CmoCh10G010850.1:exon:8273exon
CmoCh10G010850.1:exon:8274CmoCh10G010850.1:exon:8274exon
CmoCh10G010850.1:exon:8275CmoCh10G010850.1:exon:8275exon
CmoCh10G010850.1:exon:8276CmoCh10G010850.1:exon:8276exon
CmoCh10G010850.1:exon:8277CmoCh10G010850.1:exon:8277exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G010850.1:five_prime_utrCmoCh10G010850.1:five_prime_utrfive_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G010850.1:cdsCmoCh10G010850.1:cdsCDS
CmoCh10G010850.1:cdsCmoCh10G010850.1:cds_2CDS
CmoCh10G010850.1:cdsCmoCh10G010850.1:cds_3CDS
CmoCh10G010850.1:cdsCmoCh10G010850.1:cds_4CDS
CmoCh10G010850.1:cdsCmoCh10G010850.1:cds_5CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G010850.1:three_prime_utrCmoCh10G010850.1:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh10G010850.1CmoCh10G010850.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds