CmoCh10G000370.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh10G000370.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPseudouridine synthase
LocationCmo_Chr10: 184445 .. 188826 (-)
Sequence length1455
RNA-Seq ExpressionCmoCh10G000370.1
SyntenyCmoCh10G000370.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCTCTCCTCTCTTCGGTCTGACTGTGAGTATGGCTTCCGTAGCACAGCTGAATAATGACGAGCAAGAGGAGAAGGAAGAAGAAAGCATGGGAGTGGGAGAGATCGTATGGCAAACCCCTGCCAACCCCCCACAACGAAGCGATTATATATTTCACAATGGTACAGACTAGATGCCCGCTTCTGTTGGCCTTTCTTTTCGTTTTACATTCCATTTTTGTTTTAATCAGAGTTCTTTGATTAGGGAGGCGGCACGTTAGACCTTACTACTTCGAGTTCATTGCTCATGTAAGACCTTAAACTTTCAGAAATGTTCGACTATTGAATCTAATTTCAAACTCTTGATTGGAAGTTCATAGCTGCAAATTGCAGGTTAAGAATCGATGGGCCGGCAAAACCATCGTCGACTTGTTTGCTGAAGAGTTCAAAGGCCGACCTTACGAGTATTTTGTATGCTTTTTCCTTTCCGTTTTTCCTCTATGATGTGGTCATTTAGTTTCAGCTTTTGTCAGTTATTGTTTTGTTGATATCAAAATATGGAGTTCTAATATGCACTGAATATGATTCTCATGCCCTCATACATTTTTCCTTGTTTTCATGCCTTACTGCTTGGAACTTTATTTCTTAGGTTGATGCAGTCAAATGTGGTCGCATACAAGTGGATGGGGAGATGGTGCCTGTATCATATATAGTTAAATCATCTCAAAAGATAAGTCATTTCCTGCATAGGTATGTGTTCTACACGTTGTAATTTCTGGTAGGATCTTTAAGGCTTATGGTTCCCGATTGGTACAATTACGTCCAACTCCGTGAGTAGCCTGGATGTGATTCTTTTGAATATACATTTGTGTCGAATTCATTTTTATGTCAAAGCTGCACTATAAAGTTATCAAACTTGTAATAGATTTTCTACGGGCTGTAGGCATGAACCCCCGGTAATGGCTTGGGACGTGAAAATTCTCAAAGAAGAAGCAGAAGTGCTGACAGTTTGCAAGCCTGCATCTGTTCCGGTGAGTCAGATTGATAGTATGAACTTCGTATAATGCCGTTAGTTATGCAAGTTGTGCAATACATTTCTGTAAACATTTGAGTCTTAGTGAACGGATTCCTCGTCTGTTGTTTGTTTTTCTTCTAAGTTGATTTTACCCTTACTTTAGGTTCCTCTAACTAGCAAGCTCTAATGTAATATAGGTTCATCCATGTGGTCAATATAGGAAGAACACAGTTTTCGGTATACTTCAGGCAGAGCACGGTTTGGCACCTCTATTTCGTATCCAACTTCCATGTCCTTCTAGAAGCTATTGTTCTCTTATTTAGTTCTGTTATATTGATAATGGAGAAAACACTTTTAACATACTCTATCCACTGGCTTGAAGAGCAATCCTCTTCTTCCCTTGACATGACCATGTTAGCTATACATCGACTAGACCGTCTTGTCTCTGGACTTCTTATCCTTTCTAGAAACGCATCCAAAGCTGACCTTTTCAGACAACAGGTATGCCTTGTACTTAAGATTTTTCATTTAGTTATATCGTAAATGACTGGGAGATACATTTTCATGGAATTTTTATGAGGTGCATTCTTCTCAAACATTTTATTCGAGCATGCACTCTCACTACTATGTTGTTGCACATTGTTTATAACTGCTTCAGATCGTTGCAATTGGACAAAAATTAGAAGGAAAATTAACTTTAGCACAGATGAATTAGTTAAGGAATTATAACTTCAACAAAAAAATCACAAGTTCAAATTCCCACCTCGGCTCTTGGACTTTCGAGGGAAACTAAATTGTAGTTGATATGATGATTGAATGTAAAAATTTATTAGAGAGAATTTGAAATAAACATAGTTACAATTATAAATCTACTTGAATAAGAAATTTTAAGATAACAATGTCCATTTGCACAACAAGTTTTTCCCAGTAATAATCTTGCGCTGTTTCACTGTGAATAGTTAAATTCAATTTGTCATTGCTTTCTCCTCCTCTTCTGCCCATGCACAAACTAGGAACTGAAGAAACTTATATAATAGGAATGTATGCATACTGCAGATTGAGTCTGGGCTGGTGAAGAAACAGTATCTTGCGAAGGTAGTGGGTGTATTTCCAGAAGAAGAGGTATTTCTTCTTCTGTCTTAAATTATTTATCAAATTGTTCCTTCGAATGCAACTAAACGTAAGGGTTACTTTTAGATTCTTCTTGTAACAATCTAAAAGGATCCTTTTGATAAAGGGTAATTTAGAATATTGACAATGTTGAGTCTAGCTTCCAGAAGGGACCCGTGGTTGTCTTGTCCTGACTTGCTAGCTATAGGCGTGTGCATGAATGTCTGCTTGTGGCTTGTGCTTCTTATAGACATGTGGGGCTGTTAGTACAGAATGAGATGAATTACTCGGTTGTTTTTTACACTAAAGGGAAATAAATTTGTTTCAAAATCTTCTTCACTTTCAAGGATAATACCAATCTGGAGCACTGTGGTTTTTTTTTTTTTAATGTTTAGTGAAGTCACCTCATAATGTTGCTCTTCTATTTCTATTAGGCATCTCTCTGATTATCTGCTTCATTTTACCTGGACTTAATTTCAGCAAGTGGTTGATGTTAACATAAATTACAATGCTCGAGAGGGAAAAAGCACAGCGGAGGTGAGGCTTTATTTTCTTCCTTTGTCCATGTGAACTTCAGTTACTTAATAAGAGGAAGTGGATGTTAAGGAAGGAATTTACTTTTCTGGCAACCGAATATAGTAGAAGAGTCAAAAAATTGTACTTGCAAGTTAAGCAATTGAACTTACATGTTTGTGTCTAATAGGTTCTAAAAACCTTTCAAAGTATATTATAGGTTCTTGAACTTGATCCCTAAATTTTCAATCTTGTGTTTGATAGGTGTGTAACATATTCAACTTTTTTCTAAAATTAACAAATTTGTTAGTATAAATAGAATTTTGTGTGTAATAGGACATTAAACTTTCAACTTTTTGTCCAATAGATTTGTAAATTTCTAAAAATGTAGAATAGGTTAATAACTTGTTAGACACAAAAATTGAAAATTTATGAACTTATTGGATACTTTTTGAAGTTTAGATACTTACTTTATGGACCTATTTAACAGTCTCTAAATGTTGGGCCTATTTGAACCAACTATGGACCGATCTTGTAATTTAGCCTTAGAATAAAGATGGGAATTTACTTTGTAGGGAATGGTTATGTTTTCATAAAATGAGTCTGCAAAAGCCCATTAACCATCAATTTACTCTCTCTGTCTCTCTCTGTCACTAAGGACGACTGTTTGCAATTCCATAACCATAGTATTAACATGGATAAAGTTTGTGGGAGATATGTTGCTCCGTTGTCTGGTAGTTTGGAGTCCTCTTGACCGGAAGCTTCTTGGTCTGAATTTAGTATGTCACTTGATATCGATACTACAGATAGTTGATTCTTGCAGGACACTCCAACAAAGGGGAAGGCTGCTCAAACAAAGTTTACCCGCATATGTTCCGATGGAGTCCATAGCATTGTTTTATGTGAACCAATCACTGGCCGAACTCATCAAGTAAAATTTAAATCTTGACTTGTTGAAAACATTTTGCATGAGACCCCCTCCCTCAATGACATTTCGTATGTTTAAGTGATCCATGTATACTCCCACTTCCTTTTGATGTTAATATCAGTGTCTTGTGGTATACCTGCAATGCAGATCCGTGTGCATTTGCAGTATACAGGGTATCCTATAGCCAACGATGTACTGTACCTTACAAAAGAAACTTCTGGTCGTTCCGTGCAAAAAACAGGTACAGATGGGGCTGCTGCAATATCAGCCTGTTCTCGGGCACGTGATATTCAAGAAGATTGTGTTAATGCACGCAATGAAAAATCAAATGAAGATTTTAGAATTGATCCCATGTGTACAAACTGTCCAAATTTGGGTCCAAAGGGGTGAGAAACTTTGATCCCATTGGAAGCTTTGCTGGTTTGTTTCCACTCGAAACGTAATAGGGTCCATGATTAACGCATTAAACGTGCTTCGTTTTGTGCAGATACGACCGGGAAGAAGAGGGCTTGTGGCTACATTGTGTTCAGTACTCCGGACCAGGTTGGATCTACGAATGCCCATATCCAGAATGGGCTTCACTTAAATAGCATTGTTTAAGCACTTGTTAGACACAAAACTTGAAGTTGGGGATGACCCAAATTTAAATGACTAAATCTGTGATTTCACCCAAATTGTGTAAATAAAATGTAAGTGAATAGAAGTCATGATTGTGCAAGTAGAATACGGTGAGAGAAAACACAATAGATCGGTGAGGAAATTTGTTGGTAAGGTAAAAGGGAGAAGGTAGGGAAAGAAGAGAGTGTTTGAAGAATAATTAGGGGAAAAGTGGGTGACG

mRNA sequence

ATCCTCTCCTCTCTTCGGTCTGACTGTGAGTATGGCTTCCGTAGCACAGCTGAATAATGACGAGCAAGAGGAGAAGGAAGAAGAAAGCATGGGAGTGGGAGAGATCGTATGGCAAACCCCTGCCAACCCCCCACAACGAAGCGATTATATATTTCACAATGGGAGGCGGCACGTTAGACCTTACTACTTCGAGTTCATTGCTCATGTTAAGAATCGATGGGCCGGCAAAACCATCGTCGACTTGTTTGCTGAAGAGTTCAAAGGCCGACCTTACGAGTATTTTGTTGATGCAGTCAAATGTGGTCGCATACAAGTGGATGGGGAGATGGTGCCTGTATCATATATAGTTAAATCATCTCAAAAGATAAGTCATTTCCTGCATAGGCATGAACCCCCGGTAATGGCTTGGGACGTGAAAATTCTCAAAGAAGAAGCAGAAGTGCTGACAGTTTGCAAGCCTGCATCTGTTCCGGTTCATCCATGTGGTCAATATAGGAAGAACACAGTTTTCGGTATACTTCAGGCAGAGCACGGTTTGGCACCTCTATTTCCTATACATCGACTAGACCGTCTTGTCTCTGGACTTCTTATCCTTTCTAGAAACGCATCCAAAGCTGACCTTTTCAGACAACAGATTGAGTCTGGGCTGGTGAAGAAACAGTATCTTGCGAAGGTAGTGGGTGTATTTCCAGAAGAAGAGCAAGTGGTTGATGTTAACATAAATTACAATGCTCGAGAGGGAAAAAGCACAGCGGAGGACACTCCAACAAAGGGGAAGGCTGCTCAAACAAAGTTTACCCGCATATGTTCCGATGGAGTCCATAGCATTGTTTTATGTGAACCAATCACTGGCCGAACTCATCAAATCCGTGTGCATTTGCAGTATACAGGGTATCCTATAGCCAACGATGTACTGTACCTTACAAAAGAAACTTCTGGTCGTTCCGTGCAAAAAACAGGTACAGATGGGGCTGCTGCAATATCAGCCTGTTCTCGGGCACGTGATATTCAAGAAGATTGTGTTAATGCACGCAATGAAAAATCAAATGAAGATTTTAGAATTGATCCCATGTGTACAAACTGTCCAAATTTGGGTCCAAAGGGATACGACCGGGAAGAAGAGGGCTTGTGGCTACATTGTGTTCAGTACTCCGGACCAGGTTGGATCTACGAATGCCCATATCCAGAATGGGCTTCACTTAAATAGCATTGTTTAAGCACTTGTTAGACACAAAACTTGAAGTTGGGGATGACCCAAATTTAAATGACTAAATCTGTGATTTCACCCAAATTGTGTAAATAAAATGTAAGTGAATAGAAGTCATGATTGTGCAAGTAGAATACGGTGAGAGAAAACACAATAGATCGGTGAGGAAATTTGTTGGTAAGGTAAAAGGGAGAAGGTAGGGAAAGAAGAGAGTGTTTGAAGAATAATTAGGGGAAAAGTGGGTGACG

Coding sequence (CDS)

ATGGCTTCCGTAGCACAGCTGAATAATGACGAGCAAGAGGAGAAGGAAGAAGAAAGCATGGGAGTGGGAGAGATCGTATGGCAAACCCCTGCCAACCCCCCACAACGAAGCGATTATATATTTCACAATGGGAGGCGGCACGTTAGACCTTACTACTTCGAGTTCATTGCTCATGTTAAGAATCGATGGGCCGGCAAAACCATCGTCGACTTGTTTGCTGAAGAGTTCAAAGGCCGACCTTACGAGTATTTTGTTGATGCAGTCAAATGTGGTCGCATACAAGTGGATGGGGAGATGGTGCCTGTATCATATATAGTTAAATCATCTCAAAAGATAAGTCATTTCCTGCATAGGCATGAACCCCCGGTAATGGCTTGGGACGTGAAAATTCTCAAAGAAGAAGCAGAAGTGCTGACAGTTTGCAAGCCTGCATCTGTTCCGGTTCATCCATGTGGTCAATATAGGAAGAACACAGTTTTCGGTATACTTCAGGCAGAGCACGGTTTGGCACCTCTATTTCCTATACATCGACTAGACCGTCTTGTCTCTGGACTTCTTATCCTTTCTAGAAACGCATCCAAAGCTGACCTTTTCAGACAACAGATTGAGTCTGGGCTGGTGAAGAAACAGTATCTTGCGAAGGTAGTGGGTGTATTTCCAGAAGAAGAGCAAGTGGTTGATGTTAACATAAATTACAATGCTCGAGAGGGAAAAAGCACAGCGGAGGACACTCCAACAAAGGGGAAGGCTGCTCAAACAAAGTTTACCCGCATATGTTCCGATGGAGTCCATAGCATTGTTTTATGTGAACCAATCACTGGCCGAACTCATCAAATCCGTGTGCATTTGCAGTATACAGGGTATCCTATAGCCAACGATGTACTGTACCTTACAAAAGAAACTTCTGGTCGTTCCGTGCAAAAAACAGGTACAGATGGGGCTGCTGCAATATCAGCCTGTTCTCGGGCACGTGATATTCAAGAAGATTGTGTTAATGCACGCAATGAAAAATCAAATGAAGATTTTAGAATTGATCCCATGTGTACAAACTGTCCAAATTTGGGTCCAAAGGGATACGACCGGGAAGAAGAGGGCTTGTGGCTACATTGTGTTCAGTACTCCGGACCAGGTTGGATCTACGAATGCCCATATCCAGAATGGGCTTCACTTAAATAG

Protein sequence

MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDRLVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKSTAEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKETSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYDREEEGLWLHCVQYSGPGWIYECPYPEWASLK
Homology
BLAST of CmoCh10G000370.1 vs. ExPASy Swiss-Prot
Match: Q0E0Y3 (RNA pseudouridine synthase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0512300 PE=2 SV=2)

HSP 1 Score: 545.4 bits (1404), Expect = 5.1e-154
Identity = 256/381 (67.19%), Postives = 305/381 (80.05%), Query Frame = 0

Query: 21  GVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRP 80
           G   IVWQTPANPP+R DYIF +GRR+VRPYYFEFI+HVKNRWAGKTIVDLF +EFKGRP
Sbjct: 4   GPAGIVWQTPANPPERQDYIFRDGRRYVRPYYFEFISHVKNRWAGKTIVDLFTDEFKGRP 63

Query: 81  YEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAWDVKILKEEAEVLTV 140
            EY+V AVKCGR+QVD +MV   Y+V+SSQKISHFLHRHEPPV+  D+ IL+ EA+V+TV
Sbjct: 64  REYYVHAVKCGRLQVDDQMVHADYVVQSSQKISHFLHRHEPPVLGGDITILQNEADVVTV 123

Query: 141 CKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDRLVSGLLILSRNASKADLFRQ 200
           CKPASVPVHPCGQYRKNTV GILQAEHGL PLFP+HRLDRLVSGLLI ++NA KA+ FRQ
Sbjct: 124 CKPASVPVHPCGQYRKNTVVGILQAEHGLVPLFPVHRLDRLVSGLLIFAKNADKAESFRQ 183

Query: 201 QIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKSTAEDTPTKGKA-----AQTKF 260
           QIE+ L++K+Y+AKVVGVFP+ EQ V+ N+++NAREG+STAE     GKA     A TKF
Sbjct: 184 QIEASLLQKEYVAKVVGVFPDGEQTVNANVHFNAREGRSTAEVCDGDGKAPIGKQACTKF 243

Query: 261 TRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKETSGRSVQKTGTDGAA 320
            RIC+DG+HSIVLC+P+TGRTHQIRVHL++ GYPIAND +YL++  S RS + T  + A 
Sbjct: 244 QRICTDGIHSIVLCKPVTGRTHQIRVHLKHIGYPIANDEVYLSENFSPRSSKGTRINRAT 303

Query: 321 AISACSRARDIQEDCVNARNEKSNED------FRIDPMCTNCPNLGPKGYDREEEGLWLH 380
            + ACS      + C +  N  +NED      F IDPMCTNCPNL P GYD +EE LWLH
Sbjct: 304 TL-ACSLPSSDPDSCADLGNNDTNEDTEADEEFSIDPMCTNCPNLAPVGYDADEEALWLH 363

Query: 381 CVQYSGPGWIYECPYPEWASL 391
           CV+Y+GP W YECPYP+WA L
Sbjct: 364 CVRYTGPDWSYECPYPDWAFL 383

BLAST of CmoCh10G000370.1 vs. ExPASy Swiss-Prot
Match: Q9LU60 (RNA pseudouridine synthase 7 OS=Arabidopsis thaliana OX=3702 GN=At5g51140 PE=2 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.2e-144
Identity = 249/374 (66.58%), Postives = 288/374 (77.01%), Query Frame = 0

Query: 24  EIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEY 83
           +++ Q+ +   Q+ DYIFH GRRHVRPYYFEFI+HV  RW GKTIVDLFA+EFKGRP +Y
Sbjct: 32  DLLLQSSSKLSQKQDYIFHGGRRHVRPYYFEFISHVNKRWTGKTIVDLFADEFKGRPRDY 91

Query: 84  FVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAWDVKILKEEAEVLTVCKP 143
           +V AVK GRI+VDGE+VPVSYIVKSSQKI+HFLHRHEPPVM  DV IL +E +V+TVCKP
Sbjct: 92  YVGAVKSGRIKVDGEIVPVSYIVKSSQKITHFLHRHEPPVMIDDVVILHQEPDVVTVCKP 151

Query: 144 ASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDRLVSGLLILSRNASKADLFRQQIE 203
           ASVPVHPCGQYRKNT+ GIL AEH L  LFPIHRLDRLVSGLLI++R A+KAD FRQQIE
Sbjct: 152 ASVPVHPCGQYRKNTIVGILDAEHDLGTLFPIHRLDRLVSGLLIIARTAAKADFFRQQIE 211

Query: 204 SGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKSTAEDT-------PTKGKAAQTKFT 263
            G+VKK+Y+AKV+GVFPE+E +VD NINYN  EG+STAED          KGK A TKFT
Sbjct: 212 GGMVKKRYIAKVIGVFPEDEMIVDANINYNGSEGRSTAEDANSSGDDKKVKGKPACTKFT 271

Query: 264 RICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKETSGRSVQKTGTDGAAA 323
           RI ++G HS+V CEP+TGRTHQIRVHLQYTG+PIAND LYL +      +    T  A  
Sbjct: 272 RIDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYLNQH-----IDNLETYIAKR 331

Query: 324 ISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYDREEEGLWLHCVQYSGP 383
           I A  R     +D V      S+EDF IDPMCTNCP L P+GY+  +E LWLHCVQY G 
Sbjct: 332 IDAGERKIVSPDDYV-----YSSEDFSIDPMCTNCPKLIPQGYEEHDEALWLHCVQYCGT 391

Query: 384 GWIYECPYPEWASL 391
           GW YECPYP WASL
Sbjct: 392 GWEYECPYPSWASL 395

BLAST of CmoCh10G000370.1 vs. ExPASy Swiss-Prot
Match: Q09709 (Uncharacterized protein C18B11.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC18B11.02c PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.8e-65
Identity = 137/374 (36.63%), Postives = 202/374 (54.01%), Query Frame = 0

Query: 32  NPPQRSDYIFH-NGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEYFVDAVKC 91
           +P +  +Y+F  +G R V PYY+E+I   K RW GKT++++F  EF+ R   Y+  A++ 
Sbjct: 8   DPLKDCEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRN 67

Query: 92  GRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAWDVKILKEEAEVLTVCKPASVPVHP 151
           G+++V+ ++  V  ++++   +SH  HRHEPPV    V I+ E+   + + KPA VPVHP
Sbjct: 68  GQVKVNNQIGNVDTLIENGYIVSHHAHRHEPPVSDQPVGIVHEDESYVVIDKPAGVPVHP 127

Query: 152 CGQYRKNTVFGILQAEHGLAPLFPIHRLDRLVSGLLILSRNASKADLFRQQIESGLVKKQ 211
            G+Y  NTV  IL  E+  + L+P +RLDRL SGL+  S++   A+  R  + S  +KK+
Sbjct: 128 TGRYNHNTVLHILMKENKCSLLYPCNRLDRLTSGLMFFSKSPKAAEEMRVHMISKSLKKE 187

Query: 212 YLAKVVGVFPEEEQVVDVNINYNAREGKSTAEDTPTKGKAAQTKFTRICSDGVHSIVLCE 271
           Y+A+V+G FP   +V+                  P  GK A T F RI  DG  S+VLC+
Sbjct: 188 YVARVIGEFPNSGEVICDAPLLTVAPTLGLNRIHP-DGKEASTVFKRIAFDGHTSLVLCK 247

Query: 272 PITGRTHQIRVHLQYTGYPIANDVLYLTKETSGRSVQKTG----------------TDGA 331
           P+TGRTHQ+RVHLQY GYPIAND +Y  K   G S+ K G                T+ A
Sbjct: 248 PLTGRTHQLRVHLQYLGYPIANDPIYANKRVWGPSLGKNGEGSNESVIMRLSEIGKTETA 307

Query: 332 AAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYDREEEGLWLHCVQYS 389
           + +       +  E    AR +K   +      C+ C          EE G+WLH ++Y 
Sbjct: 308 SPLLQYEWYTNETEAEAKARRDKRLGELLTGKHCSECGTPLYSDPSPEELGIWLHALKYE 367

BLAST of CmoCh10G000370.1 vs. ExPASy Swiss-Prot
Match: Q8IZ73 (RNA pseudouridylate synthase domain-containing protein 2 OS=Homo sapiens OX=9606 GN=RPUSD2 PE=1 SV=2)

HSP 1 Score: 233.4 bits (594), Expect = 4.3e-60
Identity = 137/411 (33.33%), Postives = 214/411 (52.07%), Query Frame = 0

Query: 37  SDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVD 96
           + Y F  G R VRPYYF+F  + K RW G +++ +F+ EF+ +P  Y+  AV+ GR+Q++
Sbjct: 131 TSYYFEGGLRKVRPYYFDFRTYCKGRWVGHSLLHVFSTEFRAQPLAYYEAAVRAGRLQLN 190

Query: 97  GEMV-PVSYIVKSSQKISHFLHRHEPPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYR 156
            + V  ++ ++K +  + + +HRHEPPV A  +++L E  +V+ V KP+S+PVHPCG++R
Sbjct: 191 EKPVQDLNIVLKDNDFLRNTVHRHEPPVTAEPIRLLAENEDVVVVDKPSSIPVHPCGRFR 250

Query: 157 KNTVFGILQAEHGLAPLFPIHRLDRLVSGLLILSRNASKADLFRQQIESGLVKKQYLAKV 216
            NTV  IL  EH L  L P+HRLDRL SG+L+ ++ A+ ++   +Q+    ++K+Y+ +V
Sbjct: 251 HNTVIFILGKEHQLKELHPLHRLDRLTSGVLMFAKTAAVSERIHEQVRDRQLEKEYVCRV 310

Query: 217 VGVFPEEEQVVDVNINYNAREGKSTAEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGR 276
            G FP EE      I   + +      D   +GK  +T F R+  +G  S+V C P+TGR
Sbjct: 311 EGEFPTEEVTCKEPILVVSYKVGVCRVD--PRGKPCETVFQRLSYNGQSSVVRCRPLTGR 370

Query: 277 THQIRVHLQYTGYPIANDVLYLT-----KETSGRSVQKTG-------------------- 336
           THQIRVHLQ+ G+PI ND +Y +         G  + KT                     
Sbjct: 371 THQIRVHLQFLGHPILNDPIYNSVAWGPSRGRGGYIPKTNEELLRDLVAEHQAKQSLDVL 430

Query: 337 -----------TDGAAAIS------------ACSRARDIQE------DCVNARNEKSNE- 389
                      TD  A  S            A  +  ++ E      D +   +EK+ E 
Sbjct: 431 DLCEGDLSPGLTDSTAPSSELGKDDLEELAAAAQKMEEVAEAAPQELDTIALASEKAVET 490

BLAST of CmoCh10G000370.1 vs. ExPASy Swiss-Prot
Match: Q149F1 (RNA pseudouridylate synthase domain-containing protein 2 OS=Mus musculus OX=10090 GN=Rpusd2 PE=1 SV=2)

HSP 1 Score: 227.6 bits (579), Expect = 2.4e-58
Identity = 107/259 (41.31%), Postives = 167/259 (64.48%), Query Frame = 0

Query: 39  YIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGE 98
           Y F  G R VRPYYF+F  + K RW G++++ +F+ EF+ +P  Y+  AV+ GR+ ++ E
Sbjct: 146 YYFEGGLRKVRPYYFDFQTYCKGRWVGRSLLHVFSTEFRSQPLSYYEAAVRAGRLHLNEE 205

Query: 99  MV-PVSYIVKSSQKISHFLHRHEPPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKN 158
            V  +S ++K +  + + +HRHEPPV A  + +L E  +V+ + KP+S+PVHPCG++R N
Sbjct: 206 PVQDLSIVLKDNDFLRNTVHRHEPPVTAEPIHLLAENNDVVVIDKPSSIPVHPCGRFRHN 265

Query: 159 TVFGILQAEHGLAPLFPIHRLDRLVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVG 218
           TV  IL  EH L  L P+HRLDRL SG+L+ ++ A+ ++   +Q+    ++K+Y+ +V G
Sbjct: 266 TVIFILGKEHQLKELHPLHRLDRLTSGVLMFAKTAAVSEKIHEQVRDRQLEKEYVCRVAG 325

Query: 219 VFPEEEQVVDVNINYNAREGKSTAEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTH 278
            FP++E +    I   + +      D   +GK  +T F R+  +G  S+V C P+TGRTH
Sbjct: 326 EFPDKEVICKEPILVVSYKVGVCRVD--PRGKPCETVFQRLSYNGRSSVVQCRPLTGRTH 385

Query: 279 QIRVHLQYTGYPIANDVLY 297
           QIRVHLQ+ G+PI ND +Y
Sbjct: 386 QIRVHLQFLGHPILNDPIY 402

BLAST of CmoCh10G000370.1 vs. ExPASy TrEMBL
Match: A0A6J1E0D9 (Pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111429699 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 2.9e-232
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60
           MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK
Sbjct: 1   MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60

Query: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120
           NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE
Sbjct: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120

Query: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDR 180
           PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDR
Sbjct: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDR 180

Query: 181 LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240
           LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST
Sbjct: 181 LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240

Query: 241 AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 300
           AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE
Sbjct: 241 AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 300

Query: 301 TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD 360
           TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD
Sbjct: 301 TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD 360

Query: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASLK 392
           REEEGLWLHCVQYSGPGWIYECPYPEWASLK
Sbjct: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASLK 391

BLAST of CmoCh10G000370.1 vs. ExPASy TrEMBL
Match: A0A6J1JLJ0 (Pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111485688 PE=3 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 3.6e-227
Identity = 382/391 (97.70%), Postives = 385/391 (98.47%), Query Frame = 0

Query: 1   MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60
           MASV QLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK
Sbjct: 1   MASVEQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60

Query: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120
           NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE
Sbjct: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120

Query: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDR 180
           PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTV GILQAEHGLAPLFPIHRLDR
Sbjct: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDR 180

Query: 181 LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240
           LVSGLLIL+RNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST
Sbjct: 181 LVSGLLILARNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240

Query: 241 AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 300
           AEDTPTKGKAAQTKFTR+CSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIAND LYLTKE
Sbjct: 241 AEDTPTKGKAAQTKFTRVCSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDALYLTKE 300

Query: 301 TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD 360
           TSGRSVQKT  DGAAAISACSRARDIQEDCV+ARNEKSNEDFRIDPMCTNCPNL PKGYD
Sbjct: 301 TSGRSVQKTTADGAAAISACSRARDIQEDCVHARNEKSNEDFRIDPMCTNCPNLAPKGYD 360

Query: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASLK 392
           REEEGLWLHCVQYSGPGWIYECPYPEWASLK
Sbjct: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASLK 391

BLAST of CmoCh10G000370.1 vs. ExPASy TrEMBL
Match: A0A1S3CLD1 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103502297 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.4e-199
Identity = 337/391 (86.19%), Postives = 358/391 (91.56%), Query Frame = 0

Query: 7   LNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGK 66
           + +  + E+E  SMG+G IVWQTPANPPQRSDYIF NGRRHVRPYYFEFIAHVKNRWAGK
Sbjct: 1   MKSKRKREEESMSMGMGGIVWQTPANPPQRSDYIFQNGRRHVRPYYFEFIAHVKNRWAGK 60

Query: 67  TIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAW 126
           TIVDLFAEEFKGRPYEY+VDAVKCGRIQVDGE+VPVSYIVKSSQKISHFLHRHEPPVMAW
Sbjct: 61  TIVDLFAEEFKGRPYEYYVDAVKCGRIQVDGEIVPVSYIVKSSQKISHFLHRHEPPVMAW 120

Query: 127 DVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDRLVSGLL 186
            VK+LKEEAEVLTVCKPASVPVHPCGQYRKNTV GIL+AEHGLAPLFPIHRLDRLVSGLL
Sbjct: 121 GVKVLKEEAEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHRLDRLVSGLL 180

Query: 187 ILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKSTAE---- 246
           IL+RNASKADLFRQQIESGLVKKQY+A+VVGVFPEEEQVVD NINYNAREG+STAE    
Sbjct: 181 ILARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDANINYNAREGRSTAEMVDS 240

Query: 247 --DTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 306
             DTPTKGKAAQTKFTRI S+GVHSIVLCEPITGRTHQIRVHLQYTGYPIAND LYLTKE
Sbjct: 241 STDTPTKGKAAQTKFTRISSNGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDALYLTKE 300

Query: 307 TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD 366
            SGRSVQKT  D AAAIS+CS A D++EDC N   E S+EDF IDPMCTNCPNL PKGY+
Sbjct: 301 ASGRSVQKTTADRAAAISSCSPACDVEEDCANEWKENSSEDFSIDPMCTNCPNLAPKGYE 360

Query: 367 REEEGLWLHCVQYSGPGWIYECPYPEWASLK 392
            EEEGLWLHCV+YSGPGWIYECPYPEWASL+
Sbjct: 361 GEEEGLWLHCVRYSGPGWIYECPYPEWASLE 391

BLAST of CmoCh10G000370.1 vs. ExPASy TrEMBL
Match: A0A1S3CLH7 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103502297 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.9e-199
Identity = 337/392 (85.97%), Postives = 358/392 (91.33%), Query Frame = 0

Query: 7   LNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGK 66
           + +  + E+E  SMG+G IVWQTPANPPQRSDYIF NGRRHVRPYYFEFIAHVKNRWAGK
Sbjct: 1   MKSKRKREEESMSMGMGGIVWQTPANPPQRSDYIFQNGRRHVRPYYFEFIAHVKNRWAGK 60

Query: 67  TIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAW 126
           TIVDLFAEEFKGRPYEY+VDAVKCGRIQVDGE+VPVSYIVKSSQKISHFLHRHEPPVMAW
Sbjct: 61  TIVDLFAEEFKGRPYEYYVDAVKCGRIQVDGEIVPVSYIVKSSQKISHFLHRHEPPVMAW 120

Query: 127 DVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDRLVSGLL 186
            VK+LKEEAEVLTVCKPASVPVHPCGQYRKNTV GIL+AEHGLAPLFPIHRLDRLVSGLL
Sbjct: 121 GVKVLKEEAEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHRLDRLVSGLL 180

Query: 187 ILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKSTAE---- 246
           IL+RNASKADLFRQQIESGLVKKQY+A+VVGVFPEEEQVVD NINYNAREG+STAE    
Sbjct: 181 ILARNASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDANINYNAREGRSTAEQMVD 240

Query: 247 ---DTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTK 306
              DTPTKGKAAQTKFTRI S+GVHSIVLCEPITGRTHQIRVHLQYTGYPIAND LYLTK
Sbjct: 241 SSTDTPTKGKAAQTKFTRISSNGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDALYLTK 300

Query: 307 ETSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGY 366
           E SGRSVQKT  D AAAIS+CS A D++EDC N   E S+EDF IDPMCTNCPNL PKGY
Sbjct: 301 EASGRSVQKTTADRAAAISSCSPACDVEEDCANEWKENSSEDFSIDPMCTNCPNLAPKGY 360

Query: 367 DREEEGLWLHCVQYSGPGWIYECPYPEWASLK 392
           + EEEGLWLHCV+YSGPGWIYECPYPEWASL+
Sbjct: 361 EGEEEGLWLHCVRYSGPGWIYECPYPEWASLE 392

BLAST of CmoCh10G000370.1 vs. ExPASy TrEMBL
Match: A0A0A0LR57 (Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G001310 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.0e-197
Identity = 336/386 (87.05%), Postives = 350/386 (90.67%), Query Frame = 0

Query: 12  QEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDL 71
           +EE     MG+G IVWQTP NPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDL
Sbjct: 8   EEESMRMGMGMGGIVWQTPDNPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDL 67

Query: 72  FAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAWDVKIL 131
           FAEEFKGRPYEY+VDAV+CGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAW VK+L
Sbjct: 68  FAEEFKGRPYEYYVDAVECGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAWGVKVL 127

Query: 132 KEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDRLVSGLLILSRN 191
           KEE EVLTVCKPASVPVHPCGQYRKNTV GIL+AEHGLAPLFPIHRLDRLVSGLLIL+RN
Sbjct: 128 KEEEEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHRLDRLVSGLLILARN 187

Query: 192 ASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKSTAE------DTP 251
           ASKADLFRQQIESGLVKKQY+A+VVGVFPEEEQVVD NINYN REG+STAE      DT 
Sbjct: 188 ASKADLFRQQIESGLVKKQYIARVVGVFPEEEQVVDANINYNPREGRSTAEMVDSSTDTR 247

Query: 252 TKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKETSGRS 311
           TKGKAAQTKFTRI SDGVHSIV CEPITGRTHQIRVHLQYTGYPIAND LYLTKE SGRS
Sbjct: 248 TKGKAAQTKFTRISSDGVHSIVSCEPITGRTHQIRVHLQYTGYPIANDALYLTKEASGRS 307

Query: 312 VQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYDREEEG 371
           VQKT  D AAAIS CS ARD+QEDC NA  E  +EDF IDPMCTNCPNL PKGY+ EEEG
Sbjct: 308 VQKTTADRAAAISFCSPARDVQEDCANACKENWSEDFGIDPMCTNCPNLAPKGYEGEEEG 367

Query: 372 LWLHCVQYSGPGWIYECPYPEWASLK 392
           LWLHCVQYSGPGWIYECPYPEWASL+
Sbjct: 368 LWLHCVQYSGPGWIYECPYPEWASLE 393

BLAST of CmoCh10G000370.1 vs. NCBI nr
Match: XP_022921409.1 (RNA pseudouridine synthase 7-like [Cucurbita moschata])

HSP 1 Score: 813.9 bits (2101), Expect = 5.9e-232
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60
           MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK
Sbjct: 1   MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60

Query: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120
           NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE
Sbjct: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120

Query: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDR 180
           PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDR
Sbjct: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDR 180

Query: 181 LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240
           LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST
Sbjct: 181 LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240

Query: 241 AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 300
           AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE
Sbjct: 241 AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 300

Query: 301 TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD 360
           TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD
Sbjct: 301 TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD 360

Query: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASLK 392
           REEEGLWLHCVQYSGPGWIYECPYPEWASLK
Sbjct: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASLK 391

BLAST of CmoCh10G000370.1 vs. NCBI nr
Match: KAG6589362.1 (RNA pseudouridine synthase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 803.9 bits (2075), Expect = 6.1e-229
Identity = 386/390 (98.97%), Postives = 387/390 (99.23%), Query Frame = 0

Query: 1   MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60
           MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK
Sbjct: 1   MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60

Query: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120
           NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE
Sbjct: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120

Query: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDR 180
           PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTV GILQAEHGLAPLFPIHRLDR
Sbjct: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDR 180

Query: 181 LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240
           LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST
Sbjct: 181 LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240

Query: 241 AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 300
           AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIAND LYLTKE
Sbjct: 241 AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDALYLTKE 300

Query: 301 TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD 360
           TSGRSVQKT TDGAAAISACSRARDI+EDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD
Sbjct: 301 TSGRSVQKTSTDGAAAISACSRARDIEEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD 360

Query: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASL 391
           REEEGLWLHCVQYSGPGWIYECPYPEWASL
Sbjct: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASL 390

BLAST of CmoCh10G000370.1 vs. NCBI nr
Match: XP_022988439.1 (RNA pseudouridine synthase 7-like [Cucurbita maxima])

HSP 1 Score: 797.0 bits (2057), Expect = 7.5e-227
Identity = 382/391 (97.70%), Postives = 385/391 (98.47%), Query Frame = 0

Query: 1   MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60
           MASV QLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK
Sbjct: 1   MASVEQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60

Query: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120
           NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE
Sbjct: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120

Query: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDR 180
           PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTV GILQAEHGLAPLFPIHRLDR
Sbjct: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVVGILQAEHGLAPLFPIHRLDR 180

Query: 181 LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240
           LVSGLLIL+RNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST
Sbjct: 181 LVSGLLILARNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240

Query: 241 AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 300
           AEDTPTKGKAAQTKFTR+CSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIAND LYLTKE
Sbjct: 241 AEDTPTKGKAAQTKFTRVCSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDALYLTKE 300

Query: 301 TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD 360
           TSGRSVQKT  DGAAAISACSRARDIQEDCV+ARNEKSNEDFRIDPMCTNCPNL PKGYD
Sbjct: 301 TSGRSVQKTTADGAAAISACSRARDIQEDCVHARNEKSNEDFRIDPMCTNCPNLAPKGYD 360

Query: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASLK 392
           REEEGLWLHCVQYSGPGWIYECPYPEWASLK
Sbjct: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASLK 391

BLAST of CmoCh10G000370.1 vs. NCBI nr
Match: XP_023516372.1 (RNA pseudouridine synthase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788.9 bits (2036), Expect = 2.0e-224
Identity = 377/391 (96.42%), Postives = 383/391 (97.95%), Query Frame = 0

Query: 1   MASVAQLNNDEQEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60
           MASV QLNNDEQ+EKEEESMGVGEI+WQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK
Sbjct: 1   MASVEQLNNDEQKEKEEESMGVGEIIWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVK 60

Query: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHE 120
           NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQK+SHFLHRHE
Sbjct: 61  NRWAGKTIVDLFAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKMSHFLHRHE 120

Query: 121 PPVMAWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDR 180
           PPVMAW+VKILKEEAEVL VCKPASVPVHPCGQYRKNTV GILQAEHGLAPLFPIHRLDR
Sbjct: 121 PPVMAWNVKILKEEAEVLAVCKPASVPVHPCGQYRKNTVLGILQAEHGLAPLFPIHRLDR 180

Query: 181 LVSGLLILSRNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240
           LVSGLLIL+RNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST
Sbjct: 181 LVSGLLILARNASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKST 240

Query: 241 AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 300
           AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE
Sbjct: 241 AEDTPTKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 300

Query: 301 TSGRSVQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYD 360
           T+GRSVQKT  DGAAAISACSRA DIQEDCVNARNEKSNEDFRIDPMCTNCPNL PKGYD
Sbjct: 301 TTGRSVQKTTADGAAAISACSRAPDIQEDCVNARNEKSNEDFRIDPMCTNCPNLAPKGYD 360

Query: 361 REEEGLWLHCVQYSGPGWIYECPYPEWASLK 392
           REEEGLWLHCVQYSGPGWIYECPYPEW SLK
Sbjct: 361 REEEGLWLHCVQYSGPGWIYECPYPEWGSLK 391

BLAST of CmoCh10G000370.1 vs. NCBI nr
Match: XP_038879253.1 (RNA pseudouridine synthase 7 isoform X1 [Benincasa hispida])

HSP 1 Score: 717.6 bits (1851), Expect = 5.8e-203
Identity = 345/386 (89.38%), Postives = 359/386 (93.01%), Query Frame = 0

Query: 12  QEEKEEESMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDL 71
           + ++EEE MGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDL
Sbjct: 4   KRKREEEGMGVGEIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDL 63

Query: 72  FAEEFKGRPYEYFVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAWDVKIL 131
           FAEEFKGRPYEY+VDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAW VKIL
Sbjct: 64  FAEEFKGRPYEYYVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAWGVKIL 123

Query: 132 KEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDRLVSGLLILSRN 191
           KEEAEVLTVCKPASVPVHPCGQYRKNTV GIL+AEHGLAPLFPIHRLDRLVSGLLIL+RN
Sbjct: 124 KEEAEVLTVCKPASVPVHPCGQYRKNTVVGILEAEHGLAPLFPIHRLDRLVSGLLILARN 183

Query: 192 ASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKSTAE------DTP 251
           ASKADLFRQQIESGLVKKQY+A+VVGVFPE EQVVDVNINYNAREG+STAE      +TP
Sbjct: 184 ASKADLFRQQIESGLVKKQYIARVVGVFPEAEQVVDVNINYNAREGRSTAEMVDSCANTP 243

Query: 252 TKGKAAQTKFTRICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKETSGRS 311
            KGKAAQTKFTRI SDGV SIVLCEPITGRTHQIRVHLQYTGYPIAND LYLTKE SGRS
Sbjct: 244 IKGKAAQTKFTRISSDGVQSIVLCEPITGRTHQIRVHLQYTGYPIANDTLYLTKEASGRS 303

Query: 312 VQKTGTDGAAAISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYDREEEG 371
           V+KT  D AAAIS CS A DI+EDCVNAR E  +EDFRIDPMCTNCPNL PKGYD EEEG
Sbjct: 304 VEKTTADRAAAISNCSAACDIEEDCVNAREENWSEDFRIDPMCTNCPNLAPKGYDGEEEG 363

Query: 372 LWLHCVQYSGPGWIYECPYPEWASLK 392
           LWLHCVQYSGPGWIYECPYP+WASLK
Sbjct: 364 LWLHCVQYSGPGWIYECPYPQWASLK 389

BLAST of CmoCh10G000370.1 vs. TAIR 10
Match: AT5G51140.1 (Pseudouridine synthase family protein )

HSP 1 Score: 513.5 bits (1321), Expect = 1.5e-145
Identity = 249/374 (66.58%), Postives = 288/374 (77.01%), Query Frame = 0

Query: 24  EIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEY 83
           +++ Q+ +   Q+ DYIFH GRRHVRPYYFEFI+HV  RW GKTIVDLFA+EFKGRP +Y
Sbjct: 32  DLLLQSSSKLSQKQDYIFHGGRRHVRPYYFEFISHVNKRWTGKTIVDLFADEFKGRPRDY 91

Query: 84  FVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAWDVKILKEEAEVLTVCKP 143
           +V AVK GRI+VDGE+VPVSYIVKSSQKI+HFLHRHEPPVM  DV IL +E +V+TVCKP
Sbjct: 92  YVGAVKSGRIKVDGEIVPVSYIVKSSQKITHFLHRHEPPVMIDDVVILHQEPDVVTVCKP 151

Query: 144 ASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDRLVSGLLILSRNASKADLFRQQIE 203
           ASVPVHPCGQYRKNT+ GIL AEH L  LFPIHRLDRLVSGLLI++R A+KAD FRQQIE
Sbjct: 152 ASVPVHPCGQYRKNTIVGILDAEHDLGTLFPIHRLDRLVSGLLIIARTAAKADFFRQQIE 211

Query: 204 SGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKSTAEDT-------PTKGKAAQTKFT 263
            G+VKK+Y+AKV+GVFPE+E +VD NINYN  EG+STAED          KGK A TKFT
Sbjct: 212 GGMVKKRYIAKVIGVFPEDEMIVDANINYNGSEGRSTAEDANSSGDDKKVKGKPACTKFT 271

Query: 264 RICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKETSGRSVQKTGTDGAAA 323
           RI ++G HS+V CEP+TGRTHQIRVHLQYTG+PIAND LYL +      +    T  A  
Sbjct: 272 RIDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYLNQH-----IDNLETYIAKR 331

Query: 324 ISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYDREEEGLWLHCVQYSGP 383
           I A  R     +D V      S+EDF IDPMCTNCP L P+GY+  +E LWLHCVQY G 
Sbjct: 332 IDAGERKIVSPDDYV-----YSSEDFSIDPMCTNCPKLIPQGYEEHDEALWLHCVQYCGT 391

Query: 384 GWIYECPYPEWASL 391
           GW YECPYP WASL
Sbjct: 392 GWEYECPYPSWASL 395

BLAST of CmoCh10G000370.1 vs. TAIR 10
Match: AT5G51140.2 (Pseudouridine synthase family protein )

HSP 1 Score: 492.3 bits (1266), Expect = 3.7e-139
Identity = 241/365 (66.03%), Postives = 280/365 (76.71%), Query Frame = 0

Query: 24  EIVWQTPANPPQRSDYIFHNGRRHVRPYYFEFIAHVKNRWAGKTIVDLFAEEFKGRPYEY 83
           +++ Q+ +   Q+ DYIFH GRRHVRPYYFEFI+HV  RW GKTIVDLFA+EFKGRP +Y
Sbjct: 32  DLLLQSSSKLSQKQDYIFHGGRRHVRPYYFEFISHVNKRWTGKTIVDLFADEFKGRPRDY 91

Query: 84  FVDAVKCGRIQVDGEMVPVSYIVKSSQKISHFLHRHEPPVMAWDVKILKEEAEVLTVCKP 143
           +V AVK GRI+VDGE+VPVSYIVKSSQKI+HFLHRHEPPVM  DV IL +E +V+TVCKP
Sbjct: 92  YVGAVKSGRIKVDGEIVPVSYIVKSSQKITHFLHRHEPPVMIDDVVILHQEPDVVTVCKP 151

Query: 144 ASVPVHPCGQYRKNTVFGILQAEHGLAPLFPIHRLDRLVSGLLILSRNASKADLFRQQIE 203
           ASVPVHPCGQYRKNT+ GIL AEH L  LFPIHRLDRLVSGLLI++R A+KAD FRQQIE
Sbjct: 152 ASVPVHPCGQYRKNTIVGILDAEHDLGTLFPIHRLDRLVSGLLIIARTAAKADFFRQQIE 211

Query: 204 SGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKSTAEDT-------PTKGKAAQTKFT 263
            G+VKK+Y+AKV+GVFPE+E +VD NINYN  EG+STAED          KGK A TKFT
Sbjct: 212 GGMVKKRYIAKVIGVFPEDEMIVDANINYNGSEGRSTAEDANSSGDDKKVKGKPACTKFT 271

Query: 264 RICSDGVHSIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKETSGRSVQKTGTDGAAA 323
           RI ++G HS+V CEP+TGRTHQIRVHLQYTG+PIAND LYL +      +    T  A  
Sbjct: 272 RIDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYLNQH-----IDNLETYIAKR 331

Query: 324 ISACSRARDIQEDCVNARNEKSNEDFRIDPMCTNCPNLGPKGYDREEEGLWLHCVQYSGP 382
           I A  R     +D V      S+EDF IDPMCTNCP L P+GY+  +E LWLHCVQY G 
Sbjct: 332 IDAGERKIVSPDDYV-----YSSEDFSIDPMCTNCPKLIPQGYEEHDEALWLHCVQYCGT 386

BLAST of CmoCh10G000370.1 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 78.2 bits (191), Expect = 1.7e-14
Identity = 65/263 (24.71%), Postives = 122/263 (46.39%), Query Frame = 0

Query: 67  TIVDLFAEEFK-GRPYEYFVDAVKCGRIQVDGEMV-PVSYIVKSSQKISHFLHRHEPPVM 126
           T+ + +  ++K   P   ++  ++ G+IQ+DGE+V   + +++S  K+ +     + P  
Sbjct: 34  TVSEFYFTKYKSSAPLLGWIQRIQNGQIQIDGEVVKDPNTLLRSGSKLVYSRLPWKEPDT 93

Query: 127 AWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLF----------P 186
            + +++L E+ +++ + KP+ + V P G +++ TV   LQ   G    +          P
Sbjct: 94  PYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVP 153

Query: 187 IHRLDRLVSGLLILSRN-----------------ASKADLFRQQIESGLVKKQYLAKVVG 246
           +HRL R  SG+L+ ++                      +L ++      + K Y A   G
Sbjct: 154 VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG 213

Query: 247 VFPEEEQVVDVNINYNAREGKSTAEDTPT-KGKAAQTKFTRICSDGVH--SIVLCEPITG 298
           +  E+E V+   I      G +      + +GK A +K   +  D     S+V  E  +G
Sbjct: 214 IVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKVEIQSG 273

BLAST of CmoCh10G000370.1 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.5e-12
Identity = 62/256 (24.22%), Postives = 118/256 (46.09%), Query Frame = 0

Query: 67  TIVDLFAEEFK-GRPYEYFVDAVKCGRIQVDGEMV-PVSYIVKSSQKISHFLHRHEPPVM 126
           T+ + +  ++K   P   ++  ++ G+IQ+DGE+V   + +++S  K+ +     + P  
Sbjct: 34  TVSEFYFTKYKSSAPLLGWIQRIQNGQIQIDGEVVKDPNTLLRSGSKLVYSRLPWKEPDT 93

Query: 127 AWDVKILKEEAEVLTVCKPASVPVHPCGQYRKNTVFGILQAEHGLAPLF----------P 186
            + +++L E+ +++ + KP+ + V P G +++ TV   LQ   G    +          P
Sbjct: 94  PYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVP 153

Query: 187 IHRLDRLVSGLLILSRN-----------------ASKADLFRQQIESGLVKKQYLAKVVG 246
           +HRL R  SG+L+ ++                      +L ++      + K Y A   G
Sbjct: 154 VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG 213

Query: 247 VFPEEEQVVDVNINYNAREGKSTAEDTPT-KGKAAQTKFTRICSDGVH--SIVLCEPITG 291
           +  E+E V+   I      G +      + +GK A +K   +  D     S+V  E  +G
Sbjct: 214 IVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKVEIQSG 273

BLAST of CmoCh10G000370.1 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 5.0e-11
Identity = 59/239 (24.69%), Postives = 107/239 (44.77%), Query Frame = 0

Query: 93  IQVDGEMV-PVSYIVKSSQKISHFLHRHEPPVMAWDVKILKEEAEVLTVCKPASVPVHPC 152
           IQ+DGE+V   + +++S  K+ +     + P   + +++L E+ +++ + KP+ + V P 
Sbjct: 91  IQIDGEVVKDPNTLLRSGSKLVYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQVLPG 150

Query: 153 GQYRKNTVFGILQAEHGLAPLF----------PIHRLDRLVSGLLILSRN---------- 212
           G +++ TV   LQ   G    +          P+HRL R  SG+L+ ++           
Sbjct: 151 GLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRLGRGTSGILLCAKTKLAKTKLAAY 210

Query: 213 -------ASKADLFRQQIESGLVKKQYLAKVVGVFPEEEQVVDVNINYNAREGKSTAEDT 272
                      +L ++      + K Y A   G+  E+E V+   I      G +     
Sbjct: 211 FAEGTSLVGSGNLDQECGTGRKLSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYV 270

Query: 273 PT-KGKAAQTKFTRICSDGVH--SIVLCEPITGRTHQIRVHLQYTGYPIANDVLYLTKE 301
            + +GK A +K   +  D     S+V  E  +GR HQIR+HL Y G+P+    ++   E
Sbjct: 271 ASPEGKPAFSKVFVLERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVEMTMFTGSE 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0E0Y35.1e-15467.19RNA pseudouridine synthase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g05... [more]
Q9LU602.2e-14466.58RNA pseudouridine synthase 7 OS=Arabidopsis thaliana OX=3702 GN=At5g51140 PE=2 S... [more]
Q097095.8e-6536.63Uncharacterized protein C18B11.02c OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
Q8IZ734.3e-6033.33RNA pseudouridylate synthase domain-containing protein 2 OS=Homo sapiens OX=9606... [more]
Q149F12.4e-5841.31RNA pseudouridylate synthase domain-containing protein 2 OS=Mus musculus OX=1009... [more]
Match NameE-valueIdentityDescription
A0A6J1E0D92.9e-232100.00Pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111429699 PE=3 SV=1[more]
A0A6J1JLJ03.6e-22797.70Pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111485688 PE=3 SV=1[more]
A0A1S3CLD11.4e-19986.19Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103502297 PE=3 SV=1[more]
A0A1S3CLH71.9e-19985.97Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103502297 PE=3 SV=1[more]
A0A0A0LR571.0e-19787.05Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G001310 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022921409.15.9e-232100.00RNA pseudouridine synthase 7-like [Cucurbita moschata][more]
KAG6589362.16.1e-22998.97RNA pseudouridine synthase 7, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022988439.17.5e-22797.70RNA pseudouridine synthase 7-like [Cucurbita maxima][more]
XP_023516372.12.0e-22496.42RNA pseudouridine synthase 7-like [Cucurbita pepo subsp. pepo][more]
XP_038879253.15.8e-20389.38RNA pseudouridine synthase 7 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G51140.11.5e-14566.58Pseudouridine synthase family protein [more]
AT5G51140.23.7e-13966.03Pseudouridine synthase family protein [more]
AT3G52260.21.7e-1424.71Pseudouridine synthase family protein [more]
AT3G52260.11.5e-1224.22Pseudouridine synthase family protein [more]
AT3G52260.35.0e-1124.69Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006225Pseudouridine synthase, RluC/RluDTIGRFAMTIGR00005TIGR00005coord: 62..311
e-value: 1.4E-58
score: 196.5
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 137..284
e-value: 1.3E-27
score: 96.9
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 121..373
e-value: 1.8E-46
score: 160.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 18..390
NoneNo IPR availablePANTHERPTHR21600:SF62PSEUDOURIDINE SYNTHASEcoord: 18..390
NoneNo IPR availableCDDcd02557PseudoU_synth_ScRIB2coord: 113..389
e-value: 9.55001E-98
score: 288.375
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 175..189
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 129..387

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh10G000370CmoCh10G000370gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G000370.1:exon:5932CmoCh10G000370.1:exon:5932exon
CmoCh10G000370.1:exon:5931CmoCh10G000370.1:exon:5931exon
CmoCh10G000370.1:exon:5930CmoCh10G000370.1:exon:5930exon
CmoCh10G000370.1:exon:5929CmoCh10G000370.1:exon:5929exon
CmoCh10G000370.1:exon:5928CmoCh10G000370.1:exon:5928exon
CmoCh10G000370.1:exon:5927CmoCh10G000370.1:exon:5927exon
CmoCh10G000370.1:exon:5926CmoCh10G000370.1:exon:5926exon
CmoCh10G000370.1:exon:5925CmoCh10G000370.1:exon:5925exon
CmoCh10G000370.1:exon:5924CmoCh10G000370.1:exon:5924exon
CmoCh10G000370.1:exon:5923CmoCh10G000370.1:exon:5923exon
CmoCh10G000370.1:exon:5922CmoCh10G000370.1:exon:5922exon
CmoCh10G000370.1:exon:5921CmoCh10G000370.1:exon:5921exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G000370.1:three_prime_utrCmoCh10G000370.1:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_12CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_11CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_10CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_9CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_8CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_7CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_6CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_5CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_4CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_3CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cds_2CDS
CmoCh10G000370.1:cdsCmoCh10G000370.1:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G000370.1:five_prime_utrCmoCh10G000370.1:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh10G000370.1CmoCh10G000370.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding