CmoCh07G001540.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh07G001540.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionseipin-2
LocationCmo_Chr07: 842443 .. 847011 (+)
Sequence length3295
RNA-Seq ExpressionCmoCh07G001540.1
SyntenyCmoCh07G001540.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAGAAACTTTATCGGAATTGTGTGTATTTAAATCTCAAAATCGAGGAGGATGAATTTGAAGATTGTAAGAAACCCAATGTTCTGGTTTTAGATTGCGCATTTCGATACTCCATCTCGGTATGGAATCCCACAACACCAAAGATAAAGATAACGAAGACGATGATGACTTCTTCGACACTCTCGATGAGTTCCCATCGGAGGATTCTTCCGTCACCGATCAACCTCAACTTTCCACTTCACTTTCCTCCATTTCCGACTCTTCTACGGTACCTGAAACCTCGTCCGAGAATGAATTCTCATCTGTCGATTCATTACGCCATCGCCCCTCTGCTCGCCGTAGAATGGCAGGCGAGACTCCGAGCTCAGATTCCTCTATAAGTTCCCTAACAAGTACAATCGACGATTCGGGGATGATGAGCCCCGAATGGAAAACCCCAGAAATTCACCCGAATTTTATTGACGATGTAAAGAAGCTCGAGGGATCCGAGGCTTTATCCGTTCAAGTAAGCTCGTGGGGAGGTTCGAGTAGCGTGAAGGATGAGAAGAGTGAGGTATCGACGGTGACTACTGTGGAGACTAATTCGGGCGGCGAATTGGGCGTTTCGGAGGCGGAATCCGGCCATTCTTCTTCAAACCTACTTATGTTAATTGTAGGATTGCTAATCAAGGCAATTGGGGTTCAACTTAGCTTCTTCGTCTACAGTTTCTGCTTTCCCTTGTGGTTTTTATATCATTCTTACACGTTTGTTTTCCATCCTTTTCAAACAATTAAGCTTGGGAGAGCATATGTGACAGGAAAGTTATTTGGGTTTTGTGAACTTGTTACTGCTGTGGTCAGTCCTTTGATATCTGAACGGTTGAAGGAACCCAAGTCTCTTTGGAAGGCTGGACTCCGCTGTGTGTGGGGCTTTCTGTGGTCGGCTTATGTTTGTATCATTTTGTGTGGTCTCTTGATTTCGGCGCTTATATTTGGTGGATTTTTAATGAGGTTCTTAGTTCAGGAGCCTGTTAAGATCAAAGAAGTATTAAATTTTGACTACACCAAACACAGTCCTGAGGCTTATATGCCAATCTTACCTGATTCAGATGTTCTTTATGGTCGGAATTGTAAAGAAAATGTCGTTAGTGGGAAGATCCAGTCGCGTGTTATTCCTCATCATCATCATTTGCAGGCCATTGTCTCACTAACATTGCCAGAGTCTGAATATAACAGAAATCTTGGGGTCTTCCAGGTATGTCTTTATGCATTTTGTTAAAGGAATATGCCTTTTCCAATTATGACAAGTAACGTAATTTTCAGATACTTTTCTTTTTTGGTATGTTAAGTGTCGATCTTCATTGAAGTCGACTTGATATTATCTTAAACATTGCTCCAATGGATAAGATTGTGAATCATAATCCAATTCCTTTTTACCCTACCTTAACCACTGAGCGATGGAATTTGCAGTAGAAACCACTATGAAAGTGTGCTTCTGAGATGATTGTCAAAGGCTTCAATGATTAATTTGGTTGGATAGAGGATGATATGGGAATAGTAGCATAGTATTCGAGACATAGGAGCTGCTAAGAACTTGATTTATACATCTATGTTGTTTAGTCGTTTGCATTGGTTCTCTTGTGCATAACTGTTCCATGAATTATGAATAAAAGTAAACAAGAAAAGGCCACGGTAAAATAAGTTCAACAATGTGGACACCCTGCAATACAAATAAAGCTAATATAGTAATGGTTTCAGTTGTCTAAAACACAACCAAATATTAATGAAAAACTCCCTGGGGAACGTCAAAGAGTTTGCCAACTATGTGCGTACGTGATGAAGGTGGGTCGGTGAAGCCTTTTAGAATCTTGCTTAGATCAGACAAGTTCACAAGAACGTCTATATATTATTAAATTCAGTAATTGTTTGGTTTTGATTTGCCTGAAAATTCTATTTTAGTTATCAGTATCATCAATGGATGTGTATACTCTCTCTTAAGTGTGTTTGGGTAATGTTTATTATGTGATTCCTTGTGAGATATTTGATGGTCATGCTGCAGGTAAGAGTAGACTTTCTTTCTGTAAGTGGTGACGTTCTTGCAAGCTCAAGCCATCCATGCATGTTGCAGTTTAGAAGCGAGCCCATTCGCCTTTTACTGACTATCCTGAAGCTTGCACCCCTTGTTACGGGCTATATATCAGAGTCCCAGACTCTGAATCTTAAGCTTAAAGGTCTCGCTGAAGGAAATATACCGACAGCTTGTCTGCGGGTTACAATTGAACAGCGAGCAGAGTTCAGTCCTGGTGCTGGTATACCCGAAATATATGATGCATCCTTAATCCTTGAATCCGAACTTCCCCTGTTTAAAAGGATTATATGGTATTGGAGGAAGACTCTCTATGTATGGATTAGCATGACATCGTTTATGATGCAGTTGCTTTTTATGCTGGTTTGTTGTCGACCGATAATCCTTCCTAGAATGAGGATGAGGAGAAGGGATGGATCTGCGAATGTAAGCTCCACGAGGAACGACATCTAGGCATGAGGTTAGATAAGAACAGGCTCCCCTGGATATCTAATCGAAGTGTTGAATCAATAAGAATTTTGTTGCTGATATATAAAACCCAACAATTCCAATATCATTGGTTGCTCCAATACCGAATACTACATATTTAGTATGACCAATTCTTTTATTTGTTAGTTTTTTTACGTTGACTTGTATGTTCTTGGTTTGAATTTGAAGTCGATGGGGATGATGATAAGAGAGCAGATCATATTTATGACTATACAGTTCTAGAAGCCATTTCAACTCGGCACGAACGACTCGAAGGGCAATTTCTTGTGGTAACCTACATTAGAGTGAAGTTCTATGCACTGTTAACCGGGATTTTCATTGTGTGTATGATCTCACCAGGGCTACACAATTGGGGCGAAAAGAACGTTCATGTGGAGGATTCAAAACCTCGGAACTTTTATTTGTCATCATCAGAACTTGAAGCTGTGTAACGTGTGCATTGATGAACAGAGTTCATGTGAACTTCTGCGCAACCATCGAAGTTTGAACTTTTTCTAATTTAGATGTGGGATTCTTAGTTTGGTTCATATTCCAACGCGAATCTAGTTCATGTGACATCAAATACTTGTTCATTTCCATATCTTTTGATAATACAGAAGTAGAAATGTTTGGTAACTGGAAGCTCATCTATAGTAAAGTTTAAAGTACAAGAAAGAAAAGAAAAAAAGAGTCCTTTTGATATAACCCTTCGGTGTCTCAAAAGTTTTCATGGGGTGACATGACGACTTTTGAGGAACGAGGCACTTCGAGCGATCTCTCGTAGAGGTTTGTCTGTGAACCTGATGGTATTATACACTAACGCACCTGATATTGCTCCTAGAATAGGTGCTGTCATGTAAATCCATAATCCTTTGAATTGTGCAAACACTAATGCAGGCCCCAAGCTTCTGGCTGGATTCATGGATGCTCCTGTAATTGGCCTGAACAAGATGGGATACTAGATTCGTCAAGTACTCATAAAGCTTTTTACTTAAAATTACATCAACATTCAGCTTAATACCCTGCAACCATCACGTTCAGCAGCACGGTAGCACCAACAGCGAGTCCAGCAAGTTCACCAATCTGTAAGATAAAGGAAGGTTAGATTCCTTGTCCTTTCGACTCAGTTCAACATAGAAAGTTTGAGGAGAAAAATAGGAACTAACGGCTCTATTGTCAGTGGCAACACCAGAAACCACAAACATGAGGTAGAATGTGATTATGAATTCAATGACAAAGGTCTGCATGTATGAGTCGCTTGGGAGAGTTCCTGGAAAGTGGTCTTGTTTTCCATTAAAAATTAGCCTGAGTGTCCAACTTGCTAATGTTGATCCAAGAACTTGAGCCGCCAGATATGAAGGCACCTGCAGCAAATTTGAATTGGGTTGCCATCAGAATGGAATAAGCAGCACTGAAAAAGCTTAATGAGCTGCAGAGAGAGAGAGGTAGATTACCTCACTCCATGGAAATCTTCTGGTAATGGCAAAGGATATGGTGACAGCAGGGTTAAAATGAGCACCGGAAATGTGGCCAACGGAATAGATCATCACCATTACAACCAAACCCCAAACAATTGAAATCCCTGGGAAAGTGATGACTCCATCTTTGCTCAAATTCACAACCACTGATGCTTCGCCTGCAAATATCACGAAATATGTCCCCATAACCTCTGCAATCAACTGTTTCCACCAAACAAGACAAAACAAAATCAATGATTCTATATCAAATCCCAAAGCTTGAAAATGGGTTTTTTCAGACAAGAACAAAGAGAGCCAGAGACCTTCTGAATGAAAAGTACAGAGACACAATCGGATGCCGAATCATCTTCGACACTCATAGAAAGACTGTGGCGTTCATTGTTGGAAGCTGAACTCTCTGCCATGATTGATTTGGTTGAAGCTTTGAAATTGGATTGATTGGTTTGAAGAAGAATGCTGTTTTTATAGCAAATGTTTTAAAACTGGATAAAACCATAAGATCCTGTTTTTAAGATGTTGTTGAAATGTGTTCTAACAGCTGGGTTTGGTAAGAAACGAT

mRNA sequence

TGAGAAACTTTATCGGAATTGTGTGTATTTAAATCTCAAAATCGAGGAGGATGAATTTGAAGATTGTAAGAAACCCAATGTTCTGGTTTTAGATTGCGCATTTCGATACTCCATCTCGGTATGGAATCCCACAACACCAAAGATAAAGATAACGAAGACGATGATGACTTCTTCGACACTCTCGATGAGTTCCCATCGGAGGATTCTTCCGTCACCGATCAACCTCAACTTTCCACTTCACTTTCCTCCATTTCCGACTCTTCTACGGTACCTGAAACCTCGTCCGAGAATGAATTCTCATCTGTCGATTCATTACGCCATCGCCCCTCTGCTCGCCGTAGAATGGCAGGCGAGACTCCGAGCTCAGATTCCTCTATAAGTTCCCTAACAAGTACAATCGACGATTCGGGGATGATGAGCCCCGAATGGAAAACCCCAGAAATTCACCCGAATTTTATTGACGATGTAAAGAAGCTCGAGGGATCCGAGGCTTTATCCGTTCAAGTAAGCTCGTGGGGAGGTTCGAGTAGCGTGAAGGATGAGAAGAGTGAGGTATCGACGGTGACTACTGTGGAGACTAATTCGGGCGGCGAATTGGGCGTTTCGGAGGCGGAATCCGGCCATTCTTCTTCAAACCTACTTATGTTAATTGTAGGATTGCTAATCAAGGCAATTGGGGTTCAACTTAGCTTCTTCGTCTACAGTTTCTGCTTTCCCTTGTGGTTTTTATATCATTCTTACACGTTTGTTTTCCATCCTTTTCAAACAATTAAGCTTGGGAGAGCATATGTGACAGGAAAGTTATTTGGGTTTTGTGAACTTGTTACTGCTGTGGTCAGTCCTTTGATATCTGAACGGTTGAAGGAACCCAAGTCTCTTTGGAAGGCTGGACTCCGCTGTGTGTGGGGCTTTCTGTGGTCGGCTTATGTTTGTATCATTTTGTGTGGTCTCTTGATTTCGGCGCTTATATTTGGTGGATTTTTAATGAGGTTCTTAGTTCAGGAGCCTGTTAAGATCAAAGAAGTATTAAATTTTGACTACACCAAACACAGTCCTGAGGCTTATATGCCAATCTTACCTGATTCAGATGTTCTTTATGGTCGGAATTGTAAAGAAAATGTCGTTAGTGGGAAGATCCAGTCGCGTGTTATTCCTCATCATCATCATTTGCAGGCCATTGTCTCACTAACATTGCCAGAGTCTGAATATAACAGAAATCTTGGGGTCTTCCAGGTAAGAGTAGACTTTCTTTCTGTAAGTGGTGACGTTCTTGCAAGCTCAAGCCATCCATGCATGTTGCAGTTTAGAAGCGAGCCCATTCGCCTTTTACTGACTATCCTGAAGCTTGCACCCCTTGTTACGGGCTATATATCAGAGTCCCAGACTCTGAATCTTAAGCTTAAAGGTCTCGCTGAAGGAAATATACCGACAGCTTGTCTGCGGGTTACAATTGAACAGCGAGCAGAGTTCAGTCCTGGTGCTGGTATACCCGAAATATATGATGCATCCTTAATCCTTGAATCCGAACTTCCCCTGTTTAAAAGGATTATATGGTATTGGAGGAAGACTCTCTATGTATGGATTAGCATGACATCGTTTATGATGCAGTTGCTTTTTATGCTGGTTTGTTGTCGACCGATAATCCTTCCTAGAATGAGGATGAGGAGAAGGGATGGATCTGCGAATGTAAGCTCCACGAGGAACGACATCTAGGCATGAGTCGATGGGGATGATGATAAGAGAGCAGATCATATTTATGACTATACAGTTCTAGAAGCCATTTCAACTCGGCACGAACGACTCGAAGGGCAATTTCTTGTGGGCTACACAATTGGGGCGAAAAGAACGTTCATGTGGAGGATTCAAAACCTCGGAACTTTTATTTGTCATCATCAGAACTTGAAGCTGTGTAACGTGTGCATTGATGAACAGAGTTCATGTGAACTTCTGCGCAACCATCGAAGTTTGAACTTTTTCTAATTTAGATGTGGGATTCTTAGTTTGGTTCATATTCCAACGCGAATCTAGTTCATGTGACATCAAATACTTGTTCATTTCCATATCTTTTGATAATACAGAAGTAGAAATGTTTGGTAACTGGAAGCTCATCTATAGTAAAGTTTAAAGTACAAGAAAGAAAAGAAAAAAAGAGTCCTTTTGATATAACCCTTCGGTGTCTCAAAAGTTTTCATGGGGTGACATGACGACTTTTGAGGAACGAGGCACTTCGAGCGATCTCTCGTAGAGGCCCCAAGCTTCTGGCTGGATTCATGGATGCTCCTGTAATTGGCCTGAACAAGATGGGATACTAGATTCGTCAAGTACTCATAAAGCTTTTTACTTAAAATTACATCAACATTCAGCTTAATACCCTGCAACCATCACGTTCAGCAGCACGGTAGCACCAACAGCGAGTCCAGCAAGTTCACCAATCTGTAAGATAAAGGAAGAAACCACAAACATGAGGTAGAATGTGATTATGAATTCAATGACAAAGGTCTGCATGTATGAGTCGCTTGGGAGAGTTCCTGGAAAGTGGTCTTGTTTTCCATTAAAAATTAGCCTGAGTGTCCAACTTGCTAATGTTGATCCAAGAACTTGAGCCGCCAGATATGAAGGCACCTGCAGCAAATTTGAATTGGGTTGCCATCAGAATGGAATAAGCAGCACTGAAAAAGCTTAATGAGCTGCAGAGAGAGAGAGGTAGATTACCTCACTCCATGGAAATCTTCTGGTAATGGCAAAGGATATGGTGACAGCAGGGTTAAAATGAGCACCGGAAATGTGGCCAACGGAATAGATCATCACCATTACAACCAAACCCCAAACAATTGAAATCCCTGGGAAAGTGATGACTCCATCTTTGCTCAAATTCACAACCACTGATGCTTCGCCTGCAAATATCACGAAATATGTCCCCATAACCTCTGCAATCAACTGTTTCCACCAAACAAGACAAAACAAAATCAATGATTCTATATCAAATCCCAAAGCTTGAAAATGGGTTTTTTCAGACAAGAACAAAGAGAGCCAGAGACCTTCTGAATGAAAAGTACAGAGACACAATCGGATGCCGAATCATCTTCGACACTCATAGAAAGACTGTGGCGTTCATTGTTGGAAGCTGAACTCTCTGCCATGATTGATTTGGTTGAAGCTTTGAAATTGGATTGATTGGTTTGAAGAAGAATGCTGTTTTTATAGCAAATGTTTTAAAACTGGATAAAACCATAAGATCCTGTTTTTAAGATGTTGTTGAAATGTGTTCTAACAGCTGGGTTTGGTAAGAAACGAT

Coding sequence (CDS)

ATGGAATCCCACAACACCAAAGATAAAGATAACGAAGACGATGATGACTTCTTCGACACTCTCGATGAGTTCCCATCGGAGGATTCTTCCGTCACCGATCAACCTCAACTTTCCACTTCACTTTCCTCCATTTCCGACTCTTCTACGGTACCTGAAACCTCGTCCGAGAATGAATTCTCATCTGTCGATTCATTACGCCATCGCCCCTCTGCTCGCCGTAGAATGGCAGGCGAGACTCCGAGCTCAGATTCCTCTATAAGTTCCCTAACAAGTACAATCGACGATTCGGGGATGATGAGCCCCGAATGGAAAACCCCAGAAATTCACCCGAATTTTATTGACGATGTAAAGAAGCTCGAGGGATCCGAGGCTTTATCCGTTCAAGTAAGCTCGTGGGGAGGTTCGAGTAGCGTGAAGGATGAGAAGAGTGAGGTATCGACGGTGACTACTGTGGAGACTAATTCGGGCGGCGAATTGGGCGTTTCGGAGGCGGAATCCGGCCATTCTTCTTCAAACCTACTTATGTTAATTGTAGGATTGCTAATCAAGGCAATTGGGGTTCAACTTAGCTTCTTCGTCTACAGTTTCTGCTTTCCCTTGTGGTTTTTATATCATTCTTACACGTTTGTTTTCCATCCTTTTCAAACAATTAAGCTTGGGAGAGCATATGTGACAGGAAAGTTATTTGGGTTTTGTGAACTTGTTACTGCTGTGGTCAGTCCTTTGATATCTGAACGGTTGAAGGAACCCAAGTCTCTTTGGAAGGCTGGACTCCGCTGTGTGTGGGGCTTTCTGTGGTCGGCTTATGTTTGTATCATTTTGTGTGGTCTCTTGATTTCGGCGCTTATATTTGGTGGATTTTTAATGAGGTTCTTAGTTCAGGAGCCTGTTAAGATCAAAGAAGTATTAAATTTTGACTACACCAAACACAGTCCTGAGGCTTATATGCCAATCTTACCTGATTCAGATGTTCTTTATGGTCGGAATTGTAAAGAAAATGTCGTTAGTGGGAAGATCCAGTCGCGTGTTATTCCTCATCATCATCATTTGCAGGCCATTGTCTCACTAACATTGCCAGAGTCTGAATATAACAGAAATCTTGGGGTCTTCCAGGTAAGAGTAGACTTTCTTTCTGTAAGTGGTGACGTTCTTGCAAGCTCAAGCCATCCATGCATGTTGCAGTTTAGAAGCGAGCCCATTCGCCTTTTACTGACTATCCTGAAGCTTGCACCCCTTGTTACGGGCTATATATCAGAGTCCCAGACTCTGAATCTTAAGCTTAAAGGTCTCGCTGAAGGAAATATACCGACAGCTTGTCTGCGGGTTACAATTGAACAGCGAGCAGAGTTCAGTCCTGGTGCTGGTATACCCGAAATATATGATGCATCCTTAATCCTTGAATCCGAACTTCCCCTGTTTAAAAGGATTATATGGTATTGGAGGAAGACTCTCTATGTATGGATTAGCATGACATCGTTTATGATGCAGTTGCTTTTTATGCTGGTTTGTTGTCGACCGATAATCCTTCCTAGAATGAGGATGAGGAGAAGGGATGGATCTGCGAATGTAAGCTCCACGAGGAACGACATCTAG

Protein sequence

MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFSSVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLEGSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGLLIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVSPLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIKEVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPESEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISESQTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYWRKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI
Homology
BLAST of CmoCh07G001540.1 vs. ExPASy Swiss-Prot
Match: F4I340 (Seipin-2 OS=Arabidopsis thaliana OX=3702 GN=SEI2 PE=2 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 9.9e-100
Identity = 222/493 (45.03%), Postives = 307/493 (62.27%), Query Frame = 0

Query: 50  VPETSSENEFSSVDSLRHRPSARRR----MAGETPSSDSS----ISSLTSTIDDSGMMSP 109
           +P  S+ ++ SS+  LR R SA RR       ET  S SS    +   +S ++ +  +  
Sbjct: 32  LPIRSNSHQPSSL--LRRRKSAHRRDLISSDIETEPSSSSDGFDVGEKSSYVEKNAELRG 91

Query: 110 EWKTPEIHPNFIDDVKKLEGSEALSVQVSSWGGSSSVKDEK-------SEVSTVTTVETN 169
           +  T ++  +  D +      E     +SS G    V D          E STVTTV ++
Sbjct: 92  DIDTSDVIESTKDSIDLSSEKENDLDVISSSGNDMDVIDSGRNRVDPFQEESTVTTVSSD 151

Query: 170 SGGELGVSEA-----ESGHSSS-NLLMLIVGLLIKAIGVQLSFFVYSFCFPLWFLYHSYT 229
             G+   + +     E  +S+  +LL  +VGL+IKAI  Q+SF      FP W L + + 
Sbjct: 152 DQGDDDYAGSVPQFREPPNSTEWSLLGFLVGLVIKAIEFQVSFMTSLLTFPPWLLRNCFL 211

Query: 230 FVFHPFQTIKLGRAYVTGKLFGFCELVTAVVSPLISERLKEPKSLWKAGLRCVWGFLWSA 289
           F F PF TI+ GR ++  ++ G  +++   ++P    RLK+ K +     +  WG  W+ 
Sbjct: 212 FFFDPFSTIRFGRRFLMARVAGISDMIFGYMNPF---RLKDTKQMLSIVCKFGWGMFWAV 271

Query: 290 YVCIILCGLLISALIFGGFLMRFLVQEPVKIKEVLNFDYTKHSPEAYMPILPDSDVLYGR 349
           YV I+L GLL+S+L+ GG+++  +  +P ++KE LNFDYTK+SPEAY+PI   + V    
Sbjct: 272 YVGIVLFGLLVSSLMIGGYVINRIADKPFEVKETLNFDYTKNSPEAYVPISSCAGVECEG 331

Query: 350 NCKENVVSGKIQS-RVIPHHHHLQAIVSLTLPESEYNRNLGVFQVRVDFLSVSGDVLASS 409
           +CKE+    KI+  RVIP    L  I+S+TLPES YN+NLG+FQVRVDFLSV G  +AS 
Sbjct: 332 SCKESNEMSKIRGLRVIPRDQKLDIILSMTLPESAYNKNLGMFQVRVDFLSVDGQTIASI 391

Query: 410 SHPCMLQFRSEPIRLLLTILKLAPLVTGYISESQTLNLKLKGLAEGNIPTACLRVTIEQR 469
             PCML+FRSEPIRL+ T  K+ PLVTGY+SE QTL+LKLKG  E +IPTACL++ IEQR
Sbjct: 392 RRPCMLRFRSEPIRLVQTFFKVVPLVTGYVSEIQTLSLKLKGFVEKDIPTACLKIIIEQR 451

Query: 470 AEFSPGAGIPEIYDASLILESELPLFKRIIWYWRKTLYVWISMTSFMMQLLFMLVCCRPI 521
           AEF PGAGIPE+YDASL +ES LP F++IIW WRKTL+VWISM+ F+ +LLF LVCCRP+
Sbjct: 452 AEFRPGAGIPELYDASLSVESGLPFFRKIIWKWRKTLFVWISMSLFITELLFTLVCCRPL 511

BLAST of CmoCh07G001540.1 vs. ExPASy Swiss-Prot
Match: Q8L615 (Seipin-3 OS=Arabidopsis thaliana OX=3702 GN=SEI3 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.4e-93
Identity = 222/517 (42.94%), Postives = 305/517 (58.99%), Query Frame = 0

Query: 38  STSLSSISDSSTVPETSSENEF------SSVDSLRHRPSARRRMAGETPSSDSSISSLTS 97
           S S SS + SS      +E+EF      +  D L   P A  R       +DSS SS TS
Sbjct: 3   SESESSSTHSSCDRFLDAEDEFFYDSFSNHYDCLNSSPPANLRRRRLPMDTDSSSSSSTS 62

Query: 98  TIDDSGMMSPEWKTPEIHPNFID------DVKKLEGSEALSVQVSSWGGSSSVKDEKSEV 157
           +++     S   +  E+  + +D      DV   + + +    +S  G    V D  ++ 
Sbjct: 63  SLESCEKRSTVGENDELEVSLVDFETIEIDVDVTDSANSSIDSISEKGEDFEVIDSCTDT 122

Query: 158 S--------------TVTTVETNSGGELGVSEAESGHSSSNLLMLIVGLLIKAIGVQLSF 217
                          TVTT+    G      E  +G   ++    +  L+I++I  Q+S 
Sbjct: 123 EKNMGENDSGRVDPFTVTTLNDERG------EIYTGPEYTSTDWSLTSLVIRSIEFQVSL 182

Query: 218 FVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVSPLISERLKEPK 277
            +    FP W +    +FVF P++T++ GR Y+   + G C+             LK+ K
Sbjct: 183 MITFIRFPPWLISKCLSFVFDPYRTMRRGRRYLVSWIVGLCD-----------SGLKDDK 242

Query: 278 SLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIKEVLNFDYTKHS 337
            + +   R  WG   + YV I+L  LL+SA +  GF++ +L  EP+ IKE LNFDYTK S
Sbjct: 243 PVLELVRRVTWGLFCAVYVGIMLFALLVSAFMISGFVITYLAHEPLVIKESLNFDYTKSS 302

Query: 338 PEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPESEYNRNLGVFQ 397
           PEAY+PI   + V +G + KE++ +GK++          +  VS+TLPESEYNRNLG+FQ
Sbjct: 303 PEAYVPISSCAGVAFGLSGKESIETGKVKGL----KDRTEITVSMTLPESEYNRNLGMFQ 362

Query: 398 VRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISESQTLNLKLKGLA 457
           VRVDFLS SG VLASS  PCM++F SEPIRL+ T+LK+APLVTGY+SE QTLNLKLKGL 
Sbjct: 363 VRVDFLSASGHVLASSRRPCMVKFSSEPIRLVQTLLKIAPLVTGYVSEIQTLNLKLKGLV 422

Query: 458 EGN-IPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYWRKTLYVWISM 517
           E + IPTACL++ IEQRAEF PGAGIPEIYDASL LES+LP  KRIIW WRKTL+VWISM
Sbjct: 423 EKDIIPTACLKIMIEQRAEFRPGAGIPEIYDASLFLESKLPFLKRIIWNWRKTLFVWISM 482

Query: 518 TSFMMQLLFMLVCCRPIILPR--MRMRRRDGSANVSS 526
           + F+M+LLF LV  RP+I+PR   R ++RDG+ ++++
Sbjct: 483 SLFIMELLFALVFFRPLIIPRTGQRTQQRDGTHSINN 498

BLAST of CmoCh07G001540.1 vs. ExPASy Swiss-Prot
Match: Q9FFD9 (Seipin-1 OS=Arabidopsis thaliana OX=3702 GN=SEI1 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.6e-33
Identity = 87/265 (32.83%), Postives = 144/265 (54.34%), Query Frame = 0

Query: 259 RCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIKEVLNFDYTKHSPEAYMPI 318
           R  +G L + +V +++   LI A++ G  ++   V++PV +++ L FDYT+ +P A    
Sbjct: 87  RTWFGILGACHVSMVMVLALILAVVIGVGIVSLYVEKPVVVRDRLFFDYTEENPSAVFSF 146

Query: 319 LPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPESEYNRNLGVFQVRVDFLS 378
                              K +S  +P  H +   + L +PESE NR +GVFQ++V+ LS
Sbjct: 147 -----------------DKKKRSFSVPVGHSVHVSLVLWMPESEINRRIGVFQLKVELLS 206

Query: 379 VSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISESQTLNLKLKGLAEGNIPTA 438
           + G+ +A SS PCML+FRS+PIRL  T +   PL+ G  +E+QT+ +      E    T 
Sbjct: 207 LKGETIARSSQPCMLRFRSKPIRLARTFVMSVPLIAGIANEAQTMRIDALKHQEKMPRTK 266

Query: 439 CLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYWRKTLYVWISMTSFMMQLL 498
            +R T+  RA+      +P++Y+A +++ S+ P  KR+ + W+ TL VW SM  ++  L 
Sbjct: 267 AVRATLIPRAQTRT---LPQLYEAEIVINSKPPWIKRMAYNWKWTLCVWTSMYLYVAILT 326

Query: 499 FMLVCCRPIILPRMRMRRRDGSANV 524
            +L C RP++ P    R    S N+
Sbjct: 327 ALLWCFRPVLFPYTSSRTISESENL 331

BLAST of CmoCh07G001540.1 vs. ExPASy Swiss-Prot
Match: Q9Z2E9 (Seipin OS=Mus musculus OX=10090 GN=Bscl2 PE=1 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 3.7e-06
Identity = 42/159 (26.42%), Postives = 80/159 (50.31%), Query Frame = 0

Query: 271 CIILCGLLISALIFGGFLMRFLVQEPVKIKEVLNFDYTK--HSPEAYMPILPDSDVLYGR 330
           C IL  L +S  ++G F   ++    V     ++F Y     S  A +   P ++V   +
Sbjct: 36  CTILLLLWVSVFLYGSFYYSYM--PTVSHLSPVHFHYRTDCDSSTASLCSFPVANVSLAK 95

Query: 331 NCKENVVSGKIQSRVIPHHHHLQAIVSLTLPESEYNRNLGVFQVRVDFLSVSGDVLASSS 390
           + ++         RV+ +    +  + L LPES  N++LG+F V V   +  G ++++SS
Sbjct: 96  SGRD---------RVLMYGQPYRVTLELELPESPVNQDLGMFLVTVSCYTRGGRIISTSS 155

Query: 391 HPCMLQFRSEPIRLLLTILKLAPLVTGYISESQTLNLKL 428
              ML +RS+ +++L T+L  + L+ G+  + Q L ++L
Sbjct: 156 RSVMLHYRSQLLQVLDTLLFSSLLLFGFAEQKQLLEVEL 183

BLAST of CmoCh07G001540.1 vs. ExPASy Swiss-Prot
Match: Q5FVJ6 (Seipin OS=Rattus norvegicus OX=10116 GN=Bscl2 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.9e-05
Identity = 41/159 (25.79%), Postives = 80/159 (50.31%), Query Frame = 0

Query: 271 CIILCGLLISALIFGGFLMRFLVQEPVKIKEVLNFDYTK--HSPEAYMPILPDSDVLYGR 330
           C IL  L +S  ++G F   ++    V     ++F Y     S  A +   P ++V   +
Sbjct: 36  CTILLLLWVSVFLYGSFYYSYM--PTVSHLSPVHFYYRTDCDSSTASLCSFPVANVSLTK 95

Query: 331 NCKENVVSGKIQSRVIPHHHHLQAIVSLTLPESEYNRNLGVFQVRVDFLSVSGDVLASSS 390
           + ++         RV+ +    +  + L LPES  N++LG+F V V   +  G ++++SS
Sbjct: 96  SGRD---------RVLMYGQPYRVTLELELPESPVNQDLGMFLVTVSCYTRGGRIISTSS 155

Query: 391 HPCMLQFRSEPIRLLLTILKLAPLVTGYISESQTLNLKL 428
              ML +RS+ +++L T++  + L+ G+  + Q L ++L
Sbjct: 156 RSVMLHYRSQLLQMLDTLVFSSLLLFGFAEQKQLLEVEL 183

BLAST of CmoCh07G001540.1 vs. ExPASy TrEMBL
Match: A0A6J1EEQ2 (seipin-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433666 PE=4 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.6e-294
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60
           MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS
Sbjct: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60

Query: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120
           SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE
Sbjct: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180
           GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180

Query: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240
           LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS
Sbjct: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300
           PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420
           SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES
Sbjct: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420

Query: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480
           QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW
Sbjct: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480

Query: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 531
           RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI
Sbjct: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 530

BLAST of CmoCh07G001540.1 vs. ExPASy TrEMBL
Match: A0A6J1KN97 (seipin-2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496838 PE=4 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 8.8e-285
Identity = 517/530 (97.55%), Postives = 522/530 (98.49%), Query Frame = 0

Query: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60
           MESH+T  KDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSS V ETSSENEFS
Sbjct: 1   MESHDT--KDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSPVLETSSENEFS 60

Query: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120
           SV SLRHRPS+RRRMAGETPSSDSSISSLTS+IDDS MMSPEWKTPEIHPNFIDDVKKLE
Sbjct: 61  SVASLRHRPSSRRRMAGETPSSDSSISSLTSSIDDSVMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180
           GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180

Query: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240
           LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS
Sbjct: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300
           PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGK QSRVIPHHHHLQAIVSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKTQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420
           SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQF+SEPIRLLLTILKLAPLVTGYISES
Sbjct: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYISES 420

Query: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480
           QTLNLKLKGLAEGN+PTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW
Sbjct: 421 QTLNLKLKGLAEGNVPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480

Query: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 531
           RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRD SANVSSTRNDI
Sbjct: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDESANVSSTRNDI 528

BLAST of CmoCh07G001540.1 vs. ExPASy TrEMBL
Match: A0A6J1EFI9 (seipin-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433666 PE=4 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 2.9e-264
Identity = 489/530 (92.26%), Postives = 489/530 (92.26%), Query Frame = 0

Query: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60
           MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS
Sbjct: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60

Query: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120
           SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE
Sbjct: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180
           GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLI   
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLI--- 180

Query: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240
                                                 LGRAYVTGKLFGFCELVTAVVS
Sbjct: 181 --------------------------------------LGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300
           PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420
           SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES
Sbjct: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420

Query: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480
           QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW
Sbjct: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480

Query: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 531
           RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI
Sbjct: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 489

BLAST of CmoCh07G001540.1 vs. ExPASy TrEMBL
Match: A0A6J1KLL0 (seipin-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496838 PE=4 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.2e-254
Identity = 476/530 (89.81%), Postives = 481/530 (90.75%), Query Frame = 0

Query: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60
           MESH+T  KDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSS V ETSSENEFS
Sbjct: 1   MESHDT--KDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSPVLETSSENEFS 60

Query: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120
           SV SLRHRPS+RRRMAGETPSSDSSISSLTS+IDDS MMSPEWKTPEIHPNFIDDVKKLE
Sbjct: 61  SVASLRHRPSSRRRMAGETPSSDSSISSLTSSIDDSVMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180
           GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLI   
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLI--- 180

Query: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240
                                                 LGRAYVTGKLFGFCELVTAVVS
Sbjct: 181 --------------------------------------LGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300
           PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGK QSRVIPHHHHLQAIVSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKTQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420
           SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQF+SEPIRLLLTILKLAPLVTGYISES
Sbjct: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYISES 420

Query: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480
           QTLNLKLKGLAEGN+PTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW
Sbjct: 421 QTLNLKLKGLAEGNVPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480

Query: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 531
           RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRD SANVSSTRNDI
Sbjct: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDESANVSSTRNDI 487

BLAST of CmoCh07G001540.1 vs. ExPASy TrEMBL
Match: E5GC21 (Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 3.4e-220
Identity = 413/522 (79.12%), Postives = 457/522 (87.55%), Query Frame = 0

Query: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60
           MESH+T  KDNEDDDD FD+  +FPSE+ S+ DQPQLSTS S  SDSS +PE SSEN  +
Sbjct: 4   MESHDT--KDNEDDDDLFDSFHDFPSENCSLADQPQLSTSTSPSSDSSPLPEISSENVPA 63

Query: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120
            V+SLR RPS RRR+AGETP+SDSSISSLT+TIDDS + +PE K+PEIH +F DD  KLE
Sbjct: 64  PVNSLRRRPSVRRRIAGETPTSDSSISSLTTTIDDS-VKNPERKSPEIHWDFNDDGNKLE 123

Query: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180
           G E+LSVQV+SW GSSSV ++ +E STVTT E NS  ELG SE ES  SSS++L+LI GL
Sbjct: 124 GPESLSVQVNSWAGSSSVNEKDTEASTVTTAEINSEVELGASEVESRDSSSSILVLIAGL 183

Query: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240
           LIKAIGVQLSFFVYS CFPLWFLY SYTF+FHPFQTIKLGRAYV GKL G  ELV A+V 
Sbjct: 184 LIKAIGVQLSFFVYSICFPLWFLYLSYTFIFHPFQTIKLGRAYVRGKLLGVWELVVALVG 243

Query: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300
           PL+SERLKE KSLWK G+RCVWG LWS+YVCIIL GLLISALIF  FLMRFLVQEP+K+K
Sbjct: 244 PLVSERLKERKSLWKYGVRCVWGLLWSSYVCIILFGLLISALIFSAFLMRFLVQEPMKMK 303

Query: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360
           EVLNFDYTK SPEA MPILP+S+ LYG NCK+NV+SGK Q RVIP HH LQAIVSLTLPE
Sbjct: 304 EVLNFDYTKPSPEALMPILPNSNDLYGHNCKDNVLSGKTQYRVIPPHHQLQAIVSLTLPE 363

Query: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420
           SEYNRNLGVFQVRVDFLSVSGD+LASSSHPCMLQF+SEPIRLLLT+LKLAPLVTGYISES
Sbjct: 364 SEYNRNLGVFQVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTLLKLAPLVTGYISES 423

Query: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480
           QTL++KLKG  EGNIPTACLRVTIEQRAEF+PGAGIPEIY+ASLILESELPLFKRIIWYW
Sbjct: 424 QTLSIKLKGFTEGNIPTACLRVTIEQRAEFNPGAGIPEIYNASLILESELPLFKRIIWYW 483

Query: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSAN 523
           RKT+YVWISMTSFMMQLLF LVCCRP+ILP  R+RRRDGSAN
Sbjct: 484 RKTIYVWISMTSFMMQLLFTLVCCRPLILP--RIRRRDGSAN 520

BLAST of CmoCh07G001540.1 vs. NCBI nr
Match: XP_022926562.1 (seipin-2-like isoform X1 [Cucurbita moschata] >XP_022926563.1 seipin-2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.3e-294
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60
           MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS
Sbjct: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60

Query: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120
           SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE
Sbjct: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180
           GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180

Query: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240
           LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS
Sbjct: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300
           PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420
           SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES
Sbjct: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420

Query: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480
           QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW
Sbjct: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480

Query: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 531
           RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI
Sbjct: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 530

BLAST of CmoCh07G001540.1 vs. NCBI nr
Match: KAG7026505.1 (Seipin-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1019.2 bits (2634), Expect = 1.3e-293
Identity = 529/530 (99.81%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60
           MESH+TKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS
Sbjct: 1   MESHDTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60

Query: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120
           SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE
Sbjct: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180
           GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180

Query: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240
           LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS
Sbjct: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300
           PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420
           SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES
Sbjct: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420

Query: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480
           QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW
Sbjct: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480

Query: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 531
           RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI
Sbjct: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 530

BLAST of CmoCh07G001540.1 vs. NCBI nr
Match: XP_023518198.1 (seipin-3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 996.9 bits (2576), Expect = 6.7e-287
Identity = 518/530 (97.74%), Postives = 524/530 (98.87%), Query Frame = 0

Query: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60
           MESH+TKDKDNEDDDDFFDTLDEFP EDSSVTDQPQLSTSLSSISDSS VPETSSENEFS
Sbjct: 1   MESHDTKDKDNEDDDDFFDTLDEFPPEDSSVTDQPQLSTSLSSISDSSPVPETSSENEFS 60

Query: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120
           SVDSLR+RPSARRR+AGETPSSDSSISSLTSTIDDS MMSPEWKTPEIHPNFIDDVKKLE
Sbjct: 61  SVDSLRYRPSARRRIAGETPSSDSSISSLTSTIDDSVMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180
           GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSG ELGVSEAESGHSSSNLLML+VGL
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGRELGVSEAESGHSSSNLLMLMVGL 180

Query: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240
           LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS
Sbjct: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300
           PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENV SGK QSRVIPHHHHLQAIVSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVASGKTQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420
           SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQF+SEPIRLLLTILKLAPLVTGYISES
Sbjct: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYISES 420

Query: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480
           QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW
Sbjct: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480

Query: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 531
           RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSA+VSSTRNDI
Sbjct: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSADVSSTRNDI 530

BLAST of CmoCh07G001540.1 vs. NCBI nr
Match: XP_023003136.1 (seipin-2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 988.8 bits (2555), Expect = 1.8e-284
Identity = 517/530 (97.55%), Postives = 522/530 (98.49%), Query Frame = 0

Query: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60
           MESH+T  KDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSS V ETSSENEFS
Sbjct: 1   MESHDT--KDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSPVLETSSENEFS 60

Query: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120
           SV SLRHRPS+RRRMAGETPSSDSSISSLTS+IDDS MMSPEWKTPEIHPNFIDDVKKLE
Sbjct: 61  SVASLRHRPSSRRRMAGETPSSDSSISSLTSSIDDSVMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180
           GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180

Query: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240
           LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS
Sbjct: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300
           PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGK QSRVIPHHHHLQAIVSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKTQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420
           SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQF+SEPIRLLLTILKLAPLVTGYISES
Sbjct: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYISES 420

Query: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480
           QTLNLKLKGLAEGN+PTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW
Sbjct: 421 QTLNLKLKGLAEGNVPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480

Query: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 531
           RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRD SANVSSTRNDI
Sbjct: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDESANVSSTRNDI 528

BLAST of CmoCh07G001540.1 vs. NCBI nr
Match: XP_022926564.1 (seipin-2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 920.6 bits (2378), Expect = 6.1e-264
Identity = 489/530 (92.26%), Postives = 489/530 (92.26%), Query Frame = 0

Query: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60
           MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS
Sbjct: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60

Query: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120
           SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE
Sbjct: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180
           GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLI   
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLI--- 180

Query: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240
                                                 LGRAYVTGKLFGFCELVTAVVS
Sbjct: 181 --------------------------------------LGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300
           PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420
           SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES
Sbjct: 361 SEYNRNLGVFQVRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISES 420

Query: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480
           QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW
Sbjct: 421 QTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYW 480

Query: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 531
           RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI
Sbjct: 481 RKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRNDI 489

BLAST of CmoCh07G001540.1 vs. TAIR 10
Match: AT1G29760.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 365.5 bits (937), Expect = 7.0e-101
Identity = 222/493 (45.03%), Postives = 307/493 (62.27%), Query Frame = 0

Query: 50  VPETSSENEFSSVDSLRHRPSARRR----MAGETPSSDSS----ISSLTSTIDDSGMMSP 109
           +P  S+ ++ SS+  LR R SA RR       ET  S SS    +   +S ++ +  +  
Sbjct: 32  LPIRSNSHQPSSL--LRRRKSAHRRDLISSDIETEPSSSSDGFDVGEKSSYVEKNAELRG 91

Query: 110 EWKTPEIHPNFIDDVKKLEGSEALSVQVSSWGGSSSVKDEK-------SEVSTVTTVETN 169
           +  T ++  +  D +      E     +SS G    V D          E STVTTV ++
Sbjct: 92  DIDTSDVIESTKDSIDLSSEKENDLDVISSSGNDMDVIDSGRNRVDPFQEESTVTTVSSD 151

Query: 170 SGGELGVSEA-----ESGHSSS-NLLMLIVGLLIKAIGVQLSFFVYSFCFPLWFLYHSYT 229
             G+   + +     E  +S+  +LL  +VGL+IKAI  Q+SF      FP W L + + 
Sbjct: 152 DQGDDDYAGSVPQFREPPNSTEWSLLGFLVGLVIKAIEFQVSFMTSLLTFPPWLLRNCFL 211

Query: 230 FVFHPFQTIKLGRAYVTGKLFGFCELVTAVVSPLISERLKEPKSLWKAGLRCVWGFLWSA 289
           F F PF TI+ GR ++  ++ G  +++   ++P    RLK+ K +     +  WG  W+ 
Sbjct: 212 FFFDPFSTIRFGRRFLMARVAGISDMIFGYMNPF---RLKDTKQMLSIVCKFGWGMFWAV 271

Query: 290 YVCIILCGLLISALIFGGFLMRFLVQEPVKIKEVLNFDYTKHSPEAYMPILPDSDVLYGR 349
           YV I+L GLL+S+L+ GG+++  +  +P ++KE LNFDYTK+SPEAY+PI   + V    
Sbjct: 272 YVGIVLFGLLVSSLMIGGYVINRIADKPFEVKETLNFDYTKNSPEAYVPISSCAGVECEG 331

Query: 350 NCKENVVSGKIQS-RVIPHHHHLQAIVSLTLPESEYNRNLGVFQVRVDFLSVSGDVLASS 409
           +CKE+    KI+  RVIP    L  I+S+TLPES YN+NLG+FQVRVDFLSV G  +AS 
Sbjct: 332 SCKESNEMSKIRGLRVIPRDQKLDIILSMTLPESAYNKNLGMFQVRVDFLSVDGQTIASI 391

Query: 410 SHPCMLQFRSEPIRLLLTILKLAPLVTGYISESQTLNLKLKGLAEGNIPTACLRVTIEQR 469
             PCML+FRSEPIRL+ T  K+ PLVTGY+SE QTL+LKLKG  E +IPTACL++ IEQR
Sbjct: 392 RRPCMLRFRSEPIRLVQTFFKVVPLVTGYVSEIQTLSLKLKGFVEKDIPTACLKIIIEQR 451

Query: 470 AEFSPGAGIPEIYDASLILESELPLFKRIIWYWRKTLYVWISMTSFMMQLLFMLVCCRPI 521
           AEF PGAGIPE+YDASL +ES LP F++IIW WRKTL+VWISM+ F+ +LLF LVCCRP+
Sbjct: 452 AEFRPGAGIPELYDASLSVESGLPFFRKIIWKWRKTLFVWISMSLFITELLFTLVCCRPL 511

BLAST of CmoCh07G001540.1 vs. TAIR 10
Match: AT2G34380.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 345.1 bits (884), Expect = 9.8e-95
Identity = 222/517 (42.94%), Postives = 305/517 (58.99%), Query Frame = 0

Query: 38  STSLSSISDSSTVPETSSENEF------SSVDSLRHRPSARRRMAGETPSSDSSISSLTS 97
           S S SS + SS      +E+EF      +  D L   P A  R       +DSS SS TS
Sbjct: 3   SESESSSTHSSCDRFLDAEDEFFYDSFSNHYDCLNSSPPANLRRRRLPMDTDSSSSSSTS 62

Query: 98  TIDDSGMMSPEWKTPEIHPNFID------DVKKLEGSEALSVQVSSWGGSSSVKDEKSEV 157
           +++     S   +  E+  + +D      DV   + + +    +S  G    V D  ++ 
Sbjct: 63  SLESCEKRSTVGENDELEVSLVDFETIEIDVDVTDSANSSIDSISEKGEDFEVIDSCTDT 122

Query: 158 S--------------TVTTVETNSGGELGVSEAESGHSSSNLLMLIVGLLIKAIGVQLSF 217
                          TVTT+    G      E  +G   ++    +  L+I++I  Q+S 
Sbjct: 123 EKNMGENDSGRVDPFTVTTLNDERG------EIYTGPEYTSTDWSLTSLVIRSIEFQVSL 182

Query: 218 FVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVSPLISERLKEPK 277
            +    FP W +    +FVF P++T++ GR Y+   + G C+             LK+ K
Sbjct: 183 MITFIRFPPWLISKCLSFVFDPYRTMRRGRRYLVSWIVGLCD-----------SGLKDDK 242

Query: 278 SLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIKEVLNFDYTKHS 337
            + +   R  WG   + YV I+L  LL+SA +  GF++ +L  EP+ IKE LNFDYTK S
Sbjct: 243 PVLELVRRVTWGLFCAVYVGIMLFALLVSAFMISGFVITYLAHEPLVIKESLNFDYTKSS 302

Query: 338 PEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPESEYNRNLGVFQ 397
           PEAY+PI   + V +G + KE++ +GK++          +  VS+TLPESEYNRNLG+FQ
Sbjct: 303 PEAYVPISSCAGVAFGLSGKESIETGKVKGL----KDRTEITVSMTLPESEYNRNLGMFQ 362

Query: 398 VRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISESQTLNLKLKGLA 457
           VRVDFLS SG VLASS  PCM++F SEPIRL+ T+LK+APLVTGY+SE QTLNLKLKGL 
Sbjct: 363 VRVDFLSASGHVLASSRRPCMVKFSSEPIRLVQTLLKIAPLVTGYVSEIQTLNLKLKGLV 422

Query: 458 EGN-IPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYWRKTLYVWISM 517
           E + IPTACL++ IEQRAEF PGAGIPEIYDASL LES+LP  KRIIW WRKTL+VWISM
Sbjct: 423 EKDIIPTACLKIMIEQRAEFRPGAGIPEIYDASLFLESKLPFLKRIIWNWRKTLFVWISM 482

Query: 518 TSFMMQLLFMLVCCRPIILPR--MRMRRRDGSANVSS 526
           + F+M+LLF LV  RP+I+PR   R ++RDG+ ++++
Sbjct: 483 SLFIMELLFALVFFRPLIIPRTGQRTQQRDGTHSINN 498

BLAST of CmoCh07G001540.1 vs. TAIR 10
Match: AT5G16460.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 145.6 bits (366), Expect = 1.1e-34
Identity = 87/265 (32.83%), Postives = 144/265 (54.34%), Query Frame = 0

Query: 259 RCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIKEVLNFDYTKHSPEAYMPI 318
           R  +G L + +V +++   LI A++ G  ++   V++PV +++ L FDYT+ +P A    
Sbjct: 87  RTWFGILGACHVSMVMVLALILAVVIGVGIVSLYVEKPVVVRDRLFFDYTEENPSAVFSF 146

Query: 319 LPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPESEYNRNLGVFQVRVDFLS 378
                              K +S  +P  H +   + L +PESE NR +GVFQ++V+ LS
Sbjct: 147 -----------------DKKKRSFSVPVGHSVHVSLVLWMPESEINRRIGVFQLKVELLS 206

Query: 379 VSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVTGYISESQTLNLKLKGLAEGNIPTA 438
           + G+ +A SS PCML+FRS+PIRL  T +   PL+ G  +E+QT+ +      E    T 
Sbjct: 207 LKGETIARSSQPCMLRFRSKPIRLARTFVMSVPLIAGIANEAQTMRIDALKHQEKMPRTK 266

Query: 439 CLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRIIWYWRKTLYVWISMTSFMMQLL 498
            +R T+  RA+      +P++Y+A +++ S+ P  KR+ + W+ TL VW SM  ++  L 
Sbjct: 267 AVRATLIPRAQTRT---LPQLYEAEIVINSKPPWIKRMAYNWKWTLCVWTSMYLYVAILT 326

Query: 499 FMLVCCRPIILPRMRMRRRDGSANV 524
            +L C RP++ P    R    S N+
Sbjct: 327 ALLWCFRPVLFPYTSSRTISESENL 331

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I3409.9e-10045.03Seipin-2 OS=Arabidopsis thaliana OX=3702 GN=SEI2 PE=2 SV=1[more]
Q8L6151.4e-9342.94Seipin-3 OS=Arabidopsis thaliana OX=3702 GN=SEI3 PE=2 SV=1[more]
Q9FFD91.6e-3332.83Seipin-1 OS=Arabidopsis thaliana OX=3702 GN=SEI1 PE=2 SV=1[more]
Q9Z2E93.7e-0626.42Seipin OS=Mus musculus OX=10090 GN=Bscl2 PE=1 SV=2[more]
Q5FVJ61.9e-0525.79Seipin OS=Rattus norvegicus OX=10116 GN=Bscl2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EEQ21.6e-294100.00seipin-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433666 PE=4 SV=1[more]
A0A6J1KN978.8e-28597.55seipin-2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496838 PE=4 SV=1[more]
A0A6J1EFI92.9e-26492.26seipin-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433666 PE=4 SV=1[more]
A0A6J1KLL01.2e-25489.81seipin-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496838 PE=4 SV=1[more]
E5GC213.4e-22079.12Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022926562.13.3e-294100.00seipin-2-like isoform X1 [Cucurbita moschata] >XP_022926563.1 seipin-2-like isof... [more]
KAG7026505.11.3e-29399.81Seipin-2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023518198.16.7e-28797.74seipin-3-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023003136.11.8e-28497.55seipin-2-like isoform X1 [Cucurbita maxima][more]
XP_022926564.16.1e-26492.26seipin-2-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G29760.17.0e-10145.03Putative adipose-regulatory protein (Seipin) [more]
AT2G34380.19.8e-9542.94Putative adipose-regulatory protein (Seipin) [more]
AT5G16460.11.1e-3432.83Putative adipose-regulatory protein (Seipin) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009617Seipin familyPFAMPF06775Seipincoord: 277..492
e-value: 2.0E-40
score: 138.9
IPR009617Seipin familyPANTHERPTHR21212BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEINcoord: 8..529
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..162
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..107
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..98
NoneNo IPR availablePANTHERPTHR21212:SF4PROTEIN (SEIPIN), PUTATIVE-RELATEDcoord: 8..529

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh07G001540CmoCh07G001540gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh07G001540.1:exon:7563CmoCh07G001540.1:exon:7563exon
CmoCh07G001540.1:exon:7564CmoCh07G001540.1:exon:7564exon
CmoCh07G001540.1:exon:7565CmoCh07G001540.1:exon:7565exon
CmoCh07G001540.1:exon:7566CmoCh07G001540.1:exon:7566exon
CmoCh07G001540.1:exon:7567CmoCh07G001540.1:exon:7567exon
CmoCh07G001540.1:exon:7568CmoCh07G001540.1:exon:7568exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh07G001540.1:five_prime_utrCmoCh07G001540.1:five_prime_utrfive_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh07G001540.1:cdsCmoCh07G001540.1:cdsCDS
CmoCh07G001540.1:cdsCmoCh07G001540.1:cds_2CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh07G001540.1:three_prime_utrCmoCh07G001540.1:three_prime_utrthree_prime_UTR
CmoCh07G001540.1:three_prime_utrCmoCh07G001540.1:three_prime_utr_2three_prime_UTR
CmoCh07G001540.1:three_prime_utrCmoCh07G001540.1:three_prime_utr_3three_prime_UTR
CmoCh07G001540.1:three_prime_utrCmoCh07G001540.1:three_prime_utr_4three_prime_UTR
CmoCh07G001540.1:three_prime_utrCmoCh07G001540.1:three_prime_utr_5three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh07G001540.1CmoCh07G001540.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019915 lipid storage
biological_process GO:0140042 lipid droplet formation
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane