CmoCh06G008310.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh06G008310.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGamma-glutamyl phosphate reductase
LocationCmo_Chr06: 4543915 .. 4546457 (+)
Sequence length798
RNA-Seq ExpressionCmoCh06G008310.1
SyntenyCmoCh06G008310.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGCGACTCGGGATTCGGTCATCGGTTCGCATTCTCTCTCGTAGCTCAAATATTTGAGGTTTCTGTCCTCCCTTCGCCTTGATTTTTCCGGCAATTGGAAAACGAAGAAGATGAAGAGATCGGTGAGCGCTTCCAGAGTCTCCGATAAGGTGTTGTTGAGTTCGCCGTCGTTGTCGTCGTCGTTGCAATCCGCAGTCTTTAGATCTTCTAGTGTGAGTTCCGGCTTGGACTCAGAGCTTTACCTACCGACGTACAATCCGTTCTCTCACGCGGCTGAAAAAGAACGTCGCCGTCTTAAGCTTGCCAAGATCTTCATCCACTTCATTCCCGTCGTCGTCGTCTTCTGCGCCGTCGTTCTCTGGTTCTTCTCCGATCCAGGTACTGAAATTACTAGAGTGAAAGATGCTCGTAGATCGAGATCGACTATTTGATATTAAAGACGGCGAGGAATTACGAAATATAATATTTCCCCCAAAGTTATTGCTGCCCGTCCTGTAATATATCGTTAGGGATTGCAATGGATTATCGATTAACACGAGCAATCAGAGTGCTTAAAATCAATGGCCTTATTGTCGTCCTTGTCTCCATTTATGCGGCTTGTTGTTTTGATATTGGAAATGATTGGTTGTTGAACAGAAGGAAGGGGGATTGGTGGGGAAGTTGTTGTGATAGCTTATGGAAAACCGCCGAAGGACTTTAGAATAAGGATTTATTAGCATAAATTAAAGATTTCTCACCGTCCTAGCGTAGTACTTATGAAGTTTGGAGTTGGAATCAATTGCGCATGTTGTCTCTATGTAGCTAAGAAATTTTCGAATGATAGTGTGTGTATATAAACTTTGAATGCATTGCTCTCTAATATCATGTCATTAACTTGGTATCTCTTAAATGTTCGGATATTGTATGATGCAAGTAAAAGGATACATGAATTAAGTATTCCCTGGTTAACAGTTACTATTCTTCTGTTGTTCAAGTAGAATTTCATTGTATAATTTAAAGCTAAATGATAAGGGTAATGTGATTATCGGGACTGTAAACTGTTTAGCTTCTGCCATTTATTTGTTGGGGTTTCTGAGCTTGCTCAATGCAGTCAATGATCCGCTTGTTGAGTTTATTCAAATATTCATAATGTTTTACTCCATCAAGATGGTAGATGCGTGCATGTTCTGTTTGTGTAGATTCTATATATTCTACTATTTGTGACCTTAGTGATAGTATAATTAAGAAGCCTTGTACTGGTCTCACTTTTCGTTATCAAATTAGTGAATGTAAAAATCTAAACCTCCTGTATTCATCTAGGAACAGCAGTTCGAGTCTTAGCTAATTACAAATGCTTTAAGGGTCTTAGAACTCGAGTAGGCACTACTTTAGATAGCATCATATTGGGTAGGAACAGCAGTTCGAGACTTAGCTAATTAAAAATGCTTAAAGGGTCTTAGAACTTGAATAGACCGGCCCTACTTTAGATAGCATCATATTGGGTTATGGAGCCTGGTACTTATTTATAGTTTATTCAATTGTTATACTTGTAAAACTATATATGCCAAATGATCATATATATACTTACTAAAACTATATATGCAAAATGATCATATATAGTAAATGATTATTTATAGTAAAAAGCTATGTAGATAGTTATAATACTAGAGGGTTATATTCCTCTTTTAGCCAATATTTCTCCTGGTGTTATTTGTGTTTATTGAATTCATGAACATAAATCTTTAGCCAATATTATTCTCTGTCCTGTTCTTTATATTCATTTAGATCGAGTGTGTGAGTTGTTGTCTGATATCATTCTATTTTAGAATTGTTTTCCTCCTTGACTAGCATGCCAGCAAGTAAAGTCACTCTTCTATGAGATGTCTTAGGATTTAGTAAGTCTTTATGTGGCATAGTAAGAACAAAGAGGACACTTGAAGAGCACGCTCGTTTTCTTCTCCGACTTGAGTGGTGGTTCTATTACAAGGAAAATAAGTGGGAATGCCATAGTAAGCTTACATGGATATGTTCATGAACCGTTGAAAAACCATGAATTTGGGTGTTCAATATGAAGCATCAAAGTCAATAGCAGTGTTTGTTACTTTCAGGATTGTTTATGTTCAATGAATTTATCTTCTGTGTAGGACAACAAATATAGACAGAGCTGTTTCTTCAGAGACCTTACTGGAATGGTCAGCTACTTGAAGCAAAGCATGTGATCTTGATGAATGGTGTACTGAACAAGAGGACAATAAAGCCTATGCTCAGTAAGTATGCAGGCATAAGATGTCACTTTATAATAGTCTTTCCTTTGAATGATAGGCTTACTTTGCTTCGATTACTTATGTTAAAACGAACATCTCATCGTGTCGGTTGGTTATCCTCGTGTGACTAACCATTAAAACCGTAGTTTACTCGATACTTATACAGCTATGAAACTGATGTTAGCATGTGTTCATTGAGATAGAGAGAGGAGTGATTTCAAGTGTATATTCTTGACCTCTGTTCTTTGATCAAGGATATTGGTTGTGGTTGCATTGTGGATGTAGAATCTCACTTGAACT

mRNA sequence

CTGCGACTCGGGATTCGGTCATCGGTTCGCATTCTCTCTCGTAGCTCAAATATTTGAGGTTTCTGTCCTCCCTTCGCCTTGATTTTTCCGGCAATTGGAAAACGAAGAAGATGAAGAGATCGGTGAGCGCTTCCAGAGTCTCCGATAAGGTGTTGTTGAGTTCGCCGTCGTTGTCGTCGTCGTTGCAATCCGCAGTCTTTAGATCTTCTAGTGTGAGTTCCGGCTTGGACTCAGAGCTTTACCTACCGACGTACAATCCGTTCTCTCACGCGGCTGAAAAAGAACGTCGCCGTCTTAAGCTTGCCAAGATCTTCATCCACTTCATTCCCGTCGTCGTCGTCTTCTGCGCCGTCGTTCTCTGGTTCTTCTCCGATCCAGGACAACAAATATAGACAGAGCTGTTTCTTCAGAGACCTTACTGGAATGGTCAGCTACTTGAAGCAAAGCATGTGATCTTGATGAATGGTGTACTGAACAAGAGGACAATAAAGCCTATGCTCAGTAAGTATGCAGGCATAAGATGTCACTTTATAATAGTCTTTCCTTTGAATGATAGGCTTACTTTGCTTCGATTACTTATGTTAAAACGAACATCTCATCGTGTCGGTTGGTTATCCTCGTGTGACTAACCATTAAAACCGTAGTTTACTCGATACTTATACAGCTATGAAACTGATGTTAGCATGTGTTCATTGAGATAGAGAGAGGAGTGATTTCAAGTGTATATTCTTGACCTCTGTTCTTTGATCAAGGATATTGGTTGTGGTTGCATTGTGGATGTAGAATCTCACTTGAACT

Coding sequence (CDS)

ATGAAGAGATCGGTGAGCGCTTCCAGAGTCTCCGATAAGGTGTTGTTGAGTTCGCCGTCGTTGTCGTCGTCGTTGCAATCCGCAGTCTTTAGATCTTCTAGTGTGAGTTCCGGCTTGGACTCAGAGCTTTACCTACCGACGTACAATCCGTTCTCTCACGCGGCTGAAAAAGAACGTCGCCGTCTTAAGCTTGCCAAGATCTTCATCCACTTCATTCCCGTCGTCGTCGTCTTCTGCGCCGTCGTTCTCTGGTTCTTCTCCGATCCAGGACAACAAATATAG

Protein sequence

MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERRRLKLAKIFIHFIPVVVVFCAVVLWFFSDPGQQI
Homology
BLAST of CmoCh06G008310.1 vs. ExPASy TrEMBL
Match: A0A6J1FKB1 (uncharacterized protein LOC111444994 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111444994 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.7e-40
Identity = 93/93 (100.00%), Postives = 93/93 (100.00%), Query Frame = 0

Query: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60
          MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR
Sbjct: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60

Query: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDPGQQI 94
          RLKLAKIFIHFIPVVVVFCAVVLWFFSDPGQQI
Sbjct: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDPGQQI 93

BLAST of CmoCh06G008310.1 vs. ExPASy TrEMBL
Match: A0A6J1FL80 (uncharacterized protein LOC111444994 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444994 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.2e-37
Identity = 89/89 (100.00%), Postives = 89/89 (100.00%), Query Frame = 0

Query: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60
          MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR
Sbjct: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60

Query: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDP 90
          RLKLAKIFIHFIPVVVVFCAVVLWFFSDP
Sbjct: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDP 89

BLAST of CmoCh06G008310.1 vs. ExPASy TrEMBL
Match: A0A6J1KXV3 (uncharacterized protein LOC111498546 OS=Cucurbita maxima OX=3661 GN=LOC111498546 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 5.3e-36
Identity = 87/89 (97.75%), Postives = 87/89 (97.75%), Query Frame = 0

Query: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60
          MKRSVSASRVSDKVLLSSPS  SSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR
Sbjct: 1  MKRSVSASRVSDKVLLSSPSSLSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60

Query: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDP 90
          RLKLAKIFIHFIPVVVVFCAVVLWFFSDP
Sbjct: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDP 89

BLAST of CmoCh06G008310.1 vs. ExPASy TrEMBL
Match: A0A6J1E9H6 (uncharacterized protein LOC111431091 OS=Cucurbita moschata OX=3662 GN=LOC111431091 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 8.5e-26
Identity = 67/93 (72.04%), Postives = 80/93 (86.02%), Query Frame = 0

Query: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60
          M+RS SA  VSDK  L+S   SSS QSA+FRSSSVSSGL+S+++LPT+NPFSHAA K+RR
Sbjct: 1  MRRSSSACIVSDKRSLASS--SSSSQSAIFRSSSVSSGLESDVFLPTFNPFSHAAAKKRR 60

Query: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDPGQQI 94
           LKLA+IF+HFIP VV+FCAVVLWFFSDPG Q+
Sbjct: 61 HLKLARIFVHFIPFVVIFCAVVLWFFSDPGSQL 91

BLAST of CmoCh06G008310.1 vs. ExPASy TrEMBL
Match: A0A5D3CLM0 (Gamma-glutamyl phosphate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00790 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.5e-25
Identity = 66/93 (70.97%), Postives = 77/93 (82.80%), Query Frame = 0

Query: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60
          MKRS SAS VSDK  + S S SS  QSA+FRSSSVS GL+SE YLPT++PFSHAAEK+RR
Sbjct: 1  MKRSSSASIVSDKRFVYSSSSSSLSQSAIFRSSSVSFGLESEPYLPTFSPFSHAAEKKRR 60

Query: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDPGQQI 94
           LKLAKIFIH IP +++FCA+VLW FSDPG +I
Sbjct: 61 HLKLAKIFIHSIPFIILFCAIVLWLFSDPGTEI 93

BLAST of CmoCh06G008310.1 vs. TAIR 10
Match: AT2G35658.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G16840.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 75.9 bits (185), Expect = 2.0e-14
Identity = 44/93 (47.31%), Postives = 60/93 (64.52%), Query Frame = 0

Query: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60
          M+RS SAS VSD++  +SPS S S      R  + +   DS L LP Y+P SH  +K + 
Sbjct: 1  MRRSASASIVSDQLSANSPSPSPSPP----RIKTSADSDDSHLLLPRYDPNSHPGKKNKS 60

Query: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDPGQQI 94
          R + A+  IHFIP+V++ CAV+LW FS+PG QI
Sbjct: 61 RFRSAENAIHFIPIVLILCAVILWMFSNPGNQI 89

BLAST of CmoCh06G008310.1 vs. TAIR 10
Match: AT2G35658.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G16840.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 70.5 bits (171), Expect = 8.2e-13
Identity = 42/93 (45.16%), Postives = 59/93 (63.44%), Query Frame = 0

Query: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60
          M+RS SAS VSD++  +SPS S S      R  + +   DS L LP Y+P SH  +K + 
Sbjct: 1  MRRSASASIVSDQLSANSPSPSPSPP----RIKTSADSDDSHLLLPRYDPNSHPGKKNKS 60

Query: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDPGQQI 94
          R + A+  IHFIP+V++ CAV+LW FS+P  +I
Sbjct: 61 RFRSAENAIHFIPIVLILCAVILWMFSNPVAKI 89

BLAST of CmoCh06G008310.1 vs. TAIR 10
Match: AT2G35658.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G16840.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 70.5 bits (171), Expect = 8.2e-13
Identity = 41/91 (45.05%), Postives = 58/91 (63.74%), Query Frame = 0

Query: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTYNPFSHAAEKERR 60
          M+RS SAS VSD++  +SPS S S      R  + +   DS L LP Y+P SH  +K + 
Sbjct: 1  MRRSASASIVSDQLSANSPSPSPSPP----RIKTSADSDDSHLLLPRYDPNSHPGKKNKS 60

Query: 61 RLKLAKIFIHFIPVVVVFCAVVLWFFSDPGQ 92
          R + A+  IHFIP+V++ CAV+LW FS+P +
Sbjct: 61 RFRSAENAIHFIPIVLILCAVILWMFSNPAK 87

BLAST of CmoCh06G008310.1 vs. TAIR 10
Match: AT4G16840.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G35658.3); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 47.8 bits (112), Expect = 5.7e-06
Identity = 34/88 (38.64%), Postives = 50/88 (56.82%), Query Frame = 0

Query: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDS-ELYLPTYNPFSHAAEKER 60
          M+RS S SRV D      P+ S S      RS SV++  D  EL LP Y+P S A ++E+
Sbjct: 1  MRRSASGSRVLDHF----PATSRSPP----RSQSVTAMEDDVELLLPRYDPNSQAGKREK 60

Query: 61 RRLKLAKIFIHFIPVVVVFCAVVLWFFS 88
           R + ++  IH IP++++ C  +LW  S
Sbjct: 61 SRFRFSENVIHLIPLILLLCVAILWLSS 80

BLAST of CmoCh06G008310.1 vs. TAIR 10
Match: AT5G61630.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G07490.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 42.7 bits (99), Expect = 1.8e-04
Identity = 37/90 (41.11%), Postives = 46/90 (51.11%), Query Frame = 0

Query: 1  MKRSVSASRVSDKVLLSSPSLSSSLQSAVFRSSSVSSGLDSELYLPTY-NPFSHAAEKER 60
          M RS S +RV        P   SS         SV   LD E  LP+Y NP     +KE+
Sbjct: 1  MYRSASWNRV--------PEDYSSAPPKGLWMGSVIGPLD-ENELPSYNNPPEEMVKKEK 60

Query: 61 RRLKLAKIFIHFIPVVVVFCAVVLWFFSDP 90
           R K A+  IH IP V++ CA+VLW FS+P
Sbjct: 61 SRAKFAENAIHIIPFVLLACALVLWLFSNP 81

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1FKB14.7e-40100.00uncharacterized protein LOC111444994 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1FL802.2e-37100.00uncharacterized protein LOC111444994 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KXV35.3e-3697.75uncharacterized protein LOC111498546 OS=Cucurbita maxima OX=3661 GN=LOC111498546... [more]
A0A6J1E9H68.5e-2672.04uncharacterized protein LOC111431091 OS=Cucurbita moschata OX=3662 GN=LOC1114310... [more]
A0A5D3CLM05.5e-2570.97Gamma-glutamyl phosphate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT2G35658.22.0e-1447.31unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT2G35658.18.2e-1345.16unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT2G35658.38.2e-1345.05unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT4G16840.15.7e-0638.64unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G61630.11.8e-0441.11unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34189TRANSMEMBRANE PROTEINcoord: 1..91
NoneNo IPR availablePANTHERPTHR34189:SF13TRANSMEMBRANE PROTEINcoord: 1..91

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh06G008310CmoCh06G008310gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh06G008310.1:exon:4768CmoCh06G008310.1:exon:4768exon
CmoCh06G008310.1:exon:4769CmoCh06G008310.1:exon:4769exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh06G008310.1:five_prime_utrCmoCh06G008310.1:five_prime_utrfive_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh06G008310.1:cdsCmoCh06G008310.1:cdsCDS
CmoCh06G008310.1:cdsCmoCh06G008310.1:cds_2CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh06G008310.1:three_prime_utrCmoCh06G008310.1:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh06G008310.1CmoCh06G008310.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane