CmoCh02G013070.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G013070.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionubiquitin-conjugating enzyme E2 20-like
LocationCmo_Chr02: 7755628 .. 7757154 (-)
Sequence length756
RNA-Seq ExpressionCmoCh02G013070.1
SyntenyCmoCh02G013070.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGTTCACTAAAATTTTTGAATATTTACCCTTCAAATCGAAGCCCTAATTCTTCGTCTCTCTCCTTCGATTTAAGCTCTACAGTCTCCCTTCTCTCCCTACCAACGTCTAATCGATACTTCGTTGGTGGAGAAATTCCTTGGATTATTCGCGAATTCCTCGTGTCTTCTGTAATTTGGATCAGAGAGAATGGCGGCCATTAATCGATACACTGATAACACGACGCCGGCTAACCCTGTTGTTTCTCCAGCCAAGCAATCCTTACCGCCGGCCAAAACCGCGGACTCTCAGTCCGTTCTCAAAAGGTATATATATTTCCGATCTTTCGTATTTGATTTGATACGTACGAGGCTACGGATCGATCGCGGTTCTGTTTGTGTGCAATCGCCTCCGCTGTTTTGTGATATTCGGCCATTATTGAACTTTACGCTGTATGAGAGTGTTCTAGGGTTCTGGATTTAGTTTGAACTGATTTTGAGGACTGTTCTGTGGTCTTTCGCAATGATCTTTTTAGGGATTTAAGGGTAATTTGAGTGTCCTCTAAGGTTCTCTCCCTAACTTTTGCACCATAGACACATGGATCTATGTTGAACGATATACCTCGTTCTTGCGTGTTTTATATCAAGAAATTAGTGAAGAACATATATAACTAACGATCTTCCTTCAATTTTTGTTGTGTTTTCTCTGACAACAGGTTGCAGTCTGAGTTAATGGCTCTAATGGTAAGACTTGATTGAAGGATTGTTCTGTGTCATGTGTTATTTTCCGACTGTTAAATTGAAGTATTTGCTGATAGATGAATAAACTCATGGTTTTGAAATTGCAGATGAGCGGAGACTCTGGGATTTCAGCCTTTCCGGAGGAGGACAACATATTCTGCTGGAAAGGGACAATAACCGGAAGCAAAGACACAGTGTTTGAGGGAACAGAGTATAGACTATCCTTTTCGTTCCCTAACGATTACCCTTTTAAGCCACCAAAGGTGAAGTTCGAGACTGGCTGCTTTCACCCAAATGTCGACGTTTATGGCAATATTTGCTTGGACATACTTCAGGTAATTGAGCTATAGCCAAGTCTTCGACAATGAAACCGTAACTTCTATCCAGAAGTGAGTAATGTTTCATCTTTTGCAGGATAAATGGTCATCAGCCTATGATGTGAGAACAATACTTCTATCCATCCAGAGTCTGCTTGGAGGTATACTTCCTGACTTGATGGTTTTATTTCAATCTGAATGCTTCTAATTTTGCGAATGGAAAATGGATTTTGCGAATGACCCTTTACCGCCATTTACTTACAGAACCAAACACAAGCTCACCTTTAAACACCCAAGCAGCCCAATTGTGGAGCAATCAAGAAGGTGGGTTTCCATTGTAACACTAGTTTTTTTTTTCCTTCTCTCTCTGAGTTCATGTGATGAAAGTTGGCTCACTCATGTTTTCTGTGTTTTCTGCAGATTATAGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCAGCTTAGACTGTTTTNGTTTCCCAAA

mRNA sequence

ACGTTCACTAAAATTTTTGAATATTTACCCTTCAAATCGAAGCCCTAATTCTTCGTCTCTCTCCTTCGATTTAAGCTCTACAGTCTCCCTTCTCTCCCTACCAACGTCTAATCGATACTTCGTTGGTGGAGAAATTCCTTGGATTATTCGCGAATTCCTCGTGTCTTCTGTAATTTGGATCAGAGAGAATGGCGGCCATTAATCGATACACTGATAACACGACGCCGGCTAACCCTGTTGTTTCTCCAGCCAAGCAATCCTTACCGCCGGCCAAAACCGCGGACTCTCAGTCCGTTCTCAAAAGGTTGCAGTCTGAGTTAATGGCTCTAATGATGAGCGGAGACTCTGGGATTTCAGCCTTTCCGGAGGAGGACAACATATTCTGCTGGAAAGGGACAATAACCGGAAGCAAAGACACAGTGTTTGAGGGAACAGAGTATAGACTATCCTTTTCGTTCCCTAACGATTACCCTTTTAAGCCACCAAAGGTGAAGTTCGAGACTGGCTGCTTTCACCCAAATGTCGACGTTTATGGCAATATTTGCTTGGACATACTTCAGGATAAATGGTCATCAGCCTATGATGTGAGAACAATACTTCTATCCATCCAGAGTCTGCTTGGAGAACCAAACACAAGCTCACCTTTAAACACCCAAGCAGCCCAATTGTGGAGCAATCAAGAAGATTATAGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCAGCTTAGACTGTTTTNGTTTCCCAAA

Coding sequence (CDS)

ATGGCGGCCATTAATCGATACACTGATAACACGACGCCGGCTAACCCTGTTGTTTCTCCAGCCAAGCAATCCTTACCGCCGGCCAAAACCGCGGACTCTCAGTCCGTTCTCAAAAGGTTGCAGTCTGAGTTAATGGCTCTAATGATGAGCGGAGACTCTGGGATTTCAGCCTTTCCGGAGGAGGACAACATATTCTGCTGGAAAGGGACAATAACCGGAAGCAAAGACACAGTGTTTGAGGGAACAGAGTATAGACTATCCTTTTCGTTCCCTAACGATTACCCTTTTAAGCCACCAAAGGTGAAGTTCGAGACTGGCTGCTTTCACCCAAATGTCGACGTTTATGGCAATATTTGCTTGGACATACTTCAGGATAAATGGTCATCAGCCTATGATGTGAGAACAATACTTCTATCCATCCAGAGTCTGCTTGGAGAACCAAACACAAGCTCACCTTTAAACACCCAAGCAGCCCAATTGTGGAGCAATCAAGAAGATTATAGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCAGCTTAG

Protein sequence

MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTAAA
Homology
BLAST of CmoCh02G013070.1 vs. ExPASy Swiss-Prot
Match: Q8L7T3 (Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 3.8e-83
Identity = 152/180 (84.44%), Postives = 157/180 (87.22%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAA+N Y  NT    P  + +KQ   P KT DSQSVLKRLQSELM LMM G  GISAFPE
Sbjct: 1   MAAVNGYQGNTPADPPASNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGPGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLS SF NDYPFKPPKVKFET CFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQE+YRKMVEKLYKPP+A
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPSA 180

BLAST of CmoCh02G013070.1 vs. ExPASy Swiss-Prot
Match: Q9LJZ5 (Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.0e-80
Identity = 149/181 (82.32%), Postives = 155/181 (85.64%), Query Frame = 0

Query: 1   MAAINRYTDNTTPA-NPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFP 60
           MA +N YT NT  A  P  + +KQS PP KT DS SVLKRLQSELM LMM  D GISAFP
Sbjct: 1   MATVNGYTGNTPAATTPAATGSKQSAPPTKTVDSHSVLKRLQSELMGLMMGADPGISAFP 60

Query: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNIC 120
           EEDNIFCWKGTITGSKDTVFEGTEYRLS +F NDYPFK PKVKFET CFHPNVD+YGNIC
Sbjct: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNIC 120

Query: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPT 180
           LDILQDKWSSAYDVRTILLSIQSLLGEPN SSPLN QAAQLWSNQE+YRKMVEKLYKP  
Sbjct: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSNQEEYRKMVEKLYKPLN 180

BLAST of CmoCh02G013070.1 vs. ExPASy Swiss-Prot
Match: Q54XS6 (Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum OX=44689 GN=ube2c PE=3 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 2.4e-53
Identity = 97/150 (64.67%), Postives = 119/150 (79.33%), Query Frame = 0

Query: 31  ADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSF 90
           +   SV KRLQSELM LMM    GISAFP+ DNIF W GTI G+KDTV+E  E+++S  F
Sbjct: 2   SQDHSVSKRLQSELMNLMMGPSPGISAFPDGDNIFNWIGTIQGAKDTVYEQMEFKMSLKF 61

Query: 91  PNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNTS 150
           P DYP+KPP VKFET CFHPNVD  GNICLDIL+DKWS  Y+VR++L+SIQSLLGEPN  
Sbjct: 62  PTDYPYKPPTVKFETQCFHPNVDNNGNICLDILKDKWSPVYNVRSLLISIQSLLGEPNNE 121

Query: 151 SPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 181
           SPLN+ AA LWSNQ++Y+K+++K Y+  T+
Sbjct: 122 SPLNSYAASLWSNQDEYKKVLDKRYQEATS 151

BLAST of CmoCh02G013070.1 vs. ExPASy Swiss-Prot
Match: Q4R9D1 (Ubiquitin-conjugating enzyme E2 C OS=Macaca fascicularis OX=9541 GN=UBE2C PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 5.0e-51
Identity = 99/168 (58.93%), Postives = 119/168 (70.83%), Query Frame = 0

Query: 8   TDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCW 67
           + N  PA   V+ A++   P+  A    V KRLQ ELM LMMSGD GISAFPE DN+F W
Sbjct: 3   SQNRDPAATSVTAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKW 62

Query: 68  KGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKW 127
            GTI G+  TV+E   Y+LS  FP+ YP+  P VKF T C+HPNVD  GNICLDIL+DKW
Sbjct: 63  VGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKDKW 122

Query: 128 SSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLY 176
           S+ YDVRTILLSIQSLLGEPN  SPLNT AA+LW N   ++K +++ Y
Sbjct: 123 SALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETY 170

BLAST of CmoCh02G013070.1 vs. ExPASy Swiss-Prot
Match: Q95044 (Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima OX=6584 GN=UBE2C PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 8.5e-51
Identity = 94/167 (56.29%), Postives = 123/167 (73.65%), Query Frame = 0

Query: 10  NTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCWKG 69
           N  PA   V   ++      + +  SV KRLQ EL  L+MSGD GI+AFP+ DN+F W  
Sbjct: 5   NIDPAANQVRQKERPRDMTTSKERHSVSKRLQQELRTLLMSGDPGITAFPDGDNLFKWVA 64

Query: 70  TITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKWSS 129
           T+ G KDTV+E  +Y+L+  FP+DYP+KPP VKF T C+HPNVD  GNICLDIL++ W++
Sbjct: 65  TLDGPKDTVYESLKYKLTLEFPSDYPYKPPVVKFTTPCWHPNVDQSGNICLDILKENWTA 124

Query: 130 AYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYK 177
           +YDVRTILLS+QSLLGEPN +SPLN QAA +WSNQ +Y+K++ + YK
Sbjct: 125 SYDVRTILLSLQSLLGEPNNASPLNAQAADMWSNQTEYKKVLHEKYK 171

BLAST of CmoCh02G013070.1 vs. ExPASy TrEMBL
Match: A0A6J1H1C8 (ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita moschata OX=3662 GN=LOC111459191 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 7.9e-100
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 AA 183
           AA
Sbjct: 181 AA 182

BLAST of CmoCh02G013070.1 vs. ExPASy TrEMBL
Match: A0A6J1K3C1 (ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita maxima OX=3661 GN=LOC111490778 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 8.7e-99
Identity = 179/182 (98.35%), Postives = 181/182 (99.45%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTP+NPVVSPAKQSLPPAK ADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPSNPVVSPAKQSLPPAKAADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVD+YGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDIYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 AA 183
           AA
Sbjct: 181 AA 182

BLAST of CmoCh02G013070.1 vs. ExPASy TrEMBL
Match: A0A5D3BA03 (Ubiquitin-conjugating enzyme E2 20-like isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00870 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.6e-97
Identity = 177/181 (97.79%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQE+YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

BLAST of CmoCh02G013070.1 vs. ExPASy TrEMBL
Match: A0A0A0KF07 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G434330 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.6e-97
Identity = 177/181 (97.79%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQE+YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

BLAST of CmoCh02G013070.1 vs. ExPASy TrEMBL
Match: A0A1S3BLY8 (ubiquitin-conjugating enzyme E2 20 OS=Cucumis melo OX=3656 GN=LOC103491487 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.6e-97
Identity = 177/181 (97.79%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQE+YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

BLAST of CmoCh02G013070.1 vs. NCBI nr
Match: XP_022957735.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita moschata] >KAG6605929.1 Ubiquitin-conjugating enzyme E2 19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035878.1 Ubiquitin-conjugating enzyme E2 19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 372.9 bits (956), Expect = 1.6e-99
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 AA 183
           AA
Sbjct: 181 AA 182

BLAST of CmoCh02G013070.1 vs. NCBI nr
Match: XP_023533942.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 372.5 bits (955), Expect = 2.1e-99
Identity = 181/182 (99.45%), Postives = 182/182 (100.00%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVD+YGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDIYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 AA 183
           AA
Sbjct: 181 AA 182

BLAST of CmoCh02G013070.1 vs. NCBI nr
Match: XP_022995155.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita maxima])

HSP 1 Score: 369.4 bits (947), Expect = 1.8e-98
Identity = 179/182 (98.35%), Postives = 181/182 (99.45%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTP+NPVVSPAKQSLPPAK ADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPSNPVVSPAKQSLPPAKAADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVD+YGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDIYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 AA 183
           AA
Sbjct: 181 AA 182

BLAST of CmoCh02G013070.1 vs. NCBI nr
Match: XP_038900928.1 (LOW QUALITY PROTEIN: ubiquitin-conjugating enzyme E2 20 [Benincasa hispida])

HSP 1 Score: 365.9 bits (938), Expect = 2.0e-97
Identity = 178/181 (98.34%), Postives = 180/181 (99.45%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQE+YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

BLAST of CmoCh02G013070.1 vs. NCBI nr
Match: XP_004140207.1 (ubiquitin-conjugating enzyme E2 20 [Cucumis sativus] >XP_008449688.1 PREDICTED: ubiquitin-conjugating enzyme E2 20 [Cucumis melo] >KAA0061782.1 ubiquitin-conjugating enzyme E2 20-like isoform X3 [Cucumis melo var. makuwa] >KGN48113.1 hypothetical protein Csa_003824 [Cucumis sativus] >TYJ96123.1 ubiquitin-conjugating enzyme E2 20-like isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 364.0 bits (933), Expect = 7.5e-97
Identity = 177/181 (97.79%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQE+YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

BLAST of CmoCh02G013070.1 vs. TAIR 10
Match: AT1G50490.1 (ubiquitin-conjugating enzyme 20 )

HSP 1 Score: 308.9 bits (790), Expect = 2.7e-84
Identity = 152/180 (84.44%), Postives = 157/180 (87.22%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAA+N Y  NT    P  + +KQ   P KT DSQSVLKRLQSELM LMM G  GISAFPE
Sbjct: 1   MAAVNGYQGNTPADPPASNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGPGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLS SF NDYPFKPPKVKFET CFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQE+YRKMVEKLYKPP+A
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPSA 180

BLAST of CmoCh02G013070.1 vs. TAIR 10
Match: AT3G20060.1 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 300.8 bits (769), Expect = 7.3e-82
Identity = 149/181 (82.32%), Postives = 155/181 (85.64%), Query Frame = 0

Query: 1   MAAINRYTDNTTPA-NPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFP 60
           MA +N YT NT  A  P  + +KQS PP KT DS SVLKRLQSELM LMM  D GISAFP
Sbjct: 1   MATVNGYTGNTPAATTPAATGSKQSAPPTKTVDSHSVLKRLQSELMGLMMGADPGISAFP 60

Query: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNIC 120
           EEDNIFCWKGTITGSKDTVFEGTEYRLS +F NDYPFK PKVKFET CFHPNVD+YGNIC
Sbjct: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNIC 120

Query: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPT 180
           LDILQDKWSSAYDVRTILLSIQSLLGEPN SSPLN QAAQLWSNQE+YRKMVEKLYKP  
Sbjct: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSNQEEYRKMVEKLYKPLN 180

BLAST of CmoCh02G013070.1 vs. TAIR 10
Match: AT3G20060.2 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 255.8 bits (652), Expect = 2.7e-68
Identity = 121/136 (88.97%), Postives = 124/136 (91.18%), Query Frame = 0

Query: 45  MALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFE 104
           M LMM  D GISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLS +F NDYPFK PKVKFE
Sbjct: 1   MGLMMGADPGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFE 60

Query: 105 TGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQ 164
           T CFHPNVD+YGNICLDILQDKWSSAYDVRTILLSIQSLLGEPN SSPLN QAAQLWSNQ
Sbjct: 61  TCCFHPNVDLYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSNQ 120

Query: 165 EDYRKMVEKLYKPPTA 181
           E+YRKMVEKLYKP  A
Sbjct: 121 EEYRKMVEKLYKPLNA 136

BLAST of CmoCh02G013070.1 vs. TAIR 10
Match: AT2G02760.1 (ubiquiting-conjugating enzyme 2 )

HSP 1 Score: 130.6 bits (327), Expect = 1.3e-30
Identity = 62/138 (44.93%), Postives = 90/138 (65.22%), Query Frame = 0

Query: 38  KRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFK 97
           KRL  +   L     +GIS  P+++NI  W   I G  DT ++G  ++LS  F  DYP K
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 98  PPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQA 157
           PP V+F +  FHPN+   G+ICLDILQ++WS  YDV  IL SIQSLL +PN +SP N++A
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 126

Query: 158 AQLWS-NQEDYRKMVEKL 175
           A+++S ++ +Y + V ++
Sbjct: 127 ARMFSESKREYNRRVREV 144

BLAST of CmoCh02G013070.1 vs. TAIR 10
Match: AT1G14400.1 (ubiquitin carrier protein 1 )

HSP 1 Score: 130.2 bits (326), Expect = 1.7e-30
Identity = 62/135 (45.93%), Postives = 88/135 (65.19%), Query Frame = 0

Query: 38  KRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFK 97
           KRL  +   L     +GIS  P+++NI  W   I G  DT ++G  ++LS  F  DYP K
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 98  PPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQA 157
           PP V+F +  FHPN+   G+ICLDILQ++WS  YDV  IL SIQSLL +PN +SP N++A
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 126

Query: 158 AQLWS-NQEDYRKMV 172
           A+++S ++ +Y + V
Sbjct: 127 ARMYSESKREYNRRV 141

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L7T33.8e-8384.44Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2... [more]
Q9LJZ51.0e-8082.32Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1... [more]
Q54XS62.4e-5364.67Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum OX=44689 ... [more]
Q4R9D15.0e-5158.93Ubiquitin-conjugating enzyme E2 C OS=Macaca fascicularis OX=9541 GN=UBE2C PE=2 S... [more]
Q950448.5e-5156.29Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima OX=6584 GN=UBE2C PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1H1C87.9e-100100.00ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K3C18.7e-9998.35ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita maxima OX=3661 GN=LOC111490... [more]
A0A5D3BA033.6e-9797.79Ubiquitin-conjugating enzyme E2 20-like isoform X3 OS=Cucumis melo var. makuwa O... [more]
A0A0A0KF073.6e-9797.79UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G434330 PE... [more]
A0A1S3BLY83.6e-9797.79ubiquitin-conjugating enzyme E2 20 OS=Cucumis melo OX=3656 GN=LOC103491487 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_022957735.11.6e-99100.00ubiquitin-conjugating enzyme E2 20-like [Cucurbita moschata] >KAG6605929.1 Ubiqu... [more]
XP_023533942.12.1e-9999.45ubiquitin-conjugating enzyme E2 20-like [Cucurbita pepo subsp. pepo][more]
XP_022995155.11.8e-9898.35ubiquitin-conjugating enzyme E2 20-like [Cucurbita maxima][more]
XP_038900928.12.0e-9798.34LOW QUALITY PROTEIN: ubiquitin-conjugating enzyme E2 20 [Benincasa hispida][more]
XP_004140207.17.5e-9797.79ubiquitin-conjugating enzyme E2 20 [Cucumis sativus] >XP_008449688.1 PREDICTED: ... [more]
Match NameE-valueIdentityDescription
AT1G50490.12.7e-8484.44ubiquitin-conjugating enzyme 20 [more]
AT3G20060.17.3e-8282.32ubiquitin-conjugating enzyme19 [more]
AT3G20060.22.7e-6888.97ubiquitin-conjugating enzyme19 [more]
AT2G02760.11.3e-3044.93ubiquiting-conjugating enzyme 2 [more]
AT1G14400.11.7e-3045.93ubiquitin carrier protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 38..180
e-value: 5.6E-59
score: 211.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availablePANTHERPTHR24068:SF373BNAC06G03670D PROTEINcoord: 18..178
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 18..178
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 28..178
e-value: 1.9E-57
score: 195.2
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 28..174
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 39..173
e-value: 5.2E-47
score: 159.0
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 35..180
score: 48.103313
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 38..175
e-value: 2.60247E-64
score: 192.417
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 108..123

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh02G013070CmoCh02G013070gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh02G013070.1:exon:7313CmoCh02G013070.1:exon:7313exon
CmoCh02G013070.1:exon:7312CmoCh02G013070.1:exon:7312exon
CmoCh02G013070.1:exon:7311CmoCh02G013070.1:exon:7311exon
CmoCh02G013070.1:exon:7310CmoCh02G013070.1:exon:7310exon
CmoCh02G013070.1:exon:7309CmoCh02G013070.1:exon:7309exon
CmoCh02G013070.1:exon:7308CmoCh02G013070.1:exon:7308exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh02G013070.1:three_prime_utrCmoCh02G013070.1:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh02G013070.1:cdsCmoCh02G013070.1:cds_6CDS
CmoCh02G013070.1:cdsCmoCh02G013070.1:cds_5CDS
CmoCh02G013070.1:cdsCmoCh02G013070.1:cds_4CDS
CmoCh02G013070.1:cdsCmoCh02G013070.1:cds_3CDS
CmoCh02G013070.1:cdsCmoCh02G013070.1:cds_2CDS
CmoCh02G013070.1:cdsCmoCh02G013070.1:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh02G013070.1:five_prime_utrCmoCh02G013070.1:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh02G013070.1CmoCh02G013070.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031145 anaphase-promoting complex-dependent catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0030071 regulation of mitotic metaphase/anaphase transition
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity