CmoCh01G012600.1 (mRNA) Cucurbita moschata (Rifu) v1

BLAST of CmoCh01G012600.1 vs. ExPASy Swiss-Prot
Match: Q8GY87 (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=UBC26 PE=2 SV=2)

HSP 1 Score: 378.6 bits (971), Expect = 1.5e-103
Identity = 177/270 (65.56%), Postives = 209/270 (77.41%), Query Frame = 0

Query: 439 FKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEKDLPETIFVRVYESRMDLM 498
           FK+FDTV+D SDH+Y+S G S KQ  K W KKVQ +WKILE DLPE I VR  ESRMDL+
Sbjct: 244 FKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLL 303

Query: 499 RAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCGKVCLSLLNT 558
           RAVIIGA+GTPYHDGLFFFDI FP  YP  PP VHYHSGGLR+NPNLYNCGKVCLSLL T
Sbjct: 304 RAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGT 363

Query: 559 WDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDKSQRYNEDTF 618
           W G   EKWLP  STMLQ+LVSIQ LILN KPYFNEPGY    G+A GE KS+ Y+E+ F
Sbjct: 364 WAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSENVF 423

Query: 619 LLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVKGGVQDLDVG 678
           LLS+KTMVY+IRRPP+HFE++V+ H+F R HDI+ AC AY  GA +GS+VKGGVQDL+  
Sbjct: 424 LLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEA 483

Query: 679 DKSCSSHFRHSLSKLLSMLVVELKKIGVSD 709
            +S S  F+  ++  +  +V E  K+GV +
Sbjct: 484 RQSGSKKFKTDVASFMQTVVDEFVKLGVKE 513

BLAST of CmoCh01G012600.1 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.3e-102
Identity = 198/368 (53.80%), Postives = 241/368 (65.49%), Query Frame = 0

Query: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLG-AP-PTLGEA----VDMPWTQGSTQHYTNI 420
           PY   Y+ +K     F+ +     P   LG AP P  G +     + P  + +   +   
Sbjct: 224 PYAVHYSALKTA---FSNYLQPQTPDTVLGEAPAPAAGSSGLLPPNTPGFKSNAARFKEE 283

Query: 421 APDNSTSVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEE 480
            P      S    N++  L  +  FK+FD V+D SDH+Y+S G + KQ  K W K++Q+E
Sbjct: 284 PPILPPDDSRVKRNMEDYLGLYLFFKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDE 343

Query: 481 WKILEKDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHY 540
           W+ILEKDLPE IFVR YESRMDL+RAVIIGAQGTPYHDGLFFFDIFFP  YP  PP VHY
Sbjct: 344 WRILEKDLPEMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHY 403

Query: 541 HSGGLRLNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNE 600
           HSGGLR+NPNLYNCGKVCLSLL TW G   EKW+P  STMLQVLVSIQGLILN KPYFNE
Sbjct: 404 HSGGLRINPNLYNCGKVCLSLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNE 463

Query: 601 PGYEYQNGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVA 660
           PGYE   GSA GE  S+ Y+E+TF+LS+KTMVY +RRPPK+FEDF  GHFF   HD+L A
Sbjct: 464 PGYERSAGSAHGESTSKAYSENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKA 523

Query: 661 CKAYTDGAEVGSLVKGGVQDLDVGDKSCSS-HFRHSLSKLL-SMLVVELKKIGVSDCEKF 720
           C AY +GA  G LVK G  D++      SS  FR  ++  + ++L+ E   +GV   E  
Sbjct: 524 CNAYRNGATPGYLVK-GAPDVEENSAGMSSLKFRTDVATFVETVLLKEFILLGVLGLEPE 583

BLAST of CmoCh01G012600.1 vs. ExPASy Swiss-Prot
Match: Q9C918 (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 368.2 bits (944), Expect = 2.1e-100
Identity = 169/271 (62.36%), Postives = 211/271 (77.86%), Query Frame = 0

Query: 438 SFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEKDLPETIFVRVYESRMDL 497
           +FK+FD+V+D SDH+Y S G + KQ  K W KKVQ+EWKIL ++LPETIFVR  ESRMDL
Sbjct: 26  NFKRFDSVEDFSDHHYVSKGKASKQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMDL 85

Query: 498 MRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCGKVCLSLLN 557
           +RAVIIGA+GTPYHDGLFFFDI FP  YP  PP V+YHSGGLR+NPNLYNCGKVCLSLL 
Sbjct: 86  LRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNCGKVCLSLLG 145

Query: 558 TWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDKSQRYNEDT 617
           TW G   + WLP  STMLQVLVSIQ L+LN +PYFNEPGY    G+ +GE KS+ Y+E+ 
Sbjct: 146 TWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESKSKVYSENV 205

Query: 618 FLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVKGGVQDLDV 677
           FLLS+KTMVY++R+PP+HFE++V+ H+F R HDI+ AC AY  GA +GS+VKGGVQDL+ 
Sbjct: 206 FLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEQ 265

Query: 678 GDKSCSSHFRHSLSKLLSMLVVELKKIGVSD 709
             +S S  F+  ++  +  +V E  K+GV +
Sbjct: 266 ARQSGSKKFKTDVASFMQTVVDEFVKLGVKE 296

BLAST of CmoCh01G012600.1 vs. ExPASy Swiss-Prot
Match: F4HPP7 (Putative ubiquitin-conjugating enzyme E2 39 OS=Arabidopsis thaliana OX=3702 GN=UBC39 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 8.2e-89
Identity = 161/276 (58.33%), Postives = 197/276 (71.38%), Query Frame = 0

Query: 439 FKQFDTVDDHSDHYYSSNG---------FSMKQEPKKWTKKVQEEWKILEKDLPETIFVR 498
           FK+FDTV+D SDH+Y++ G            K  PK W K +Q+EWKIL+K+LPETIFVR
Sbjct: 21  FKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKNLPETIFVR 80

Query: 499 VYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCG 558
             ESR+DL+RAVIIGA+GTPYHDGLFFFDI FP  YP  PPKVHYHSGGLR+NPNLY CG
Sbjct: 81  ACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGLRINPNLYKCG 140

Query: 559 KVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDK 618
           KVCLSL++TW G+  EKWLP  STMLQ+LVSIQ LILN KPY+NEPGYE   G+ +GE  
Sbjct: 141 KVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSMGTPLGESY 200

Query: 619 SQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVK 678
           S+ Y+E+ F+ S+KTM         HFE+FVR HFF R HDI+ AC AY DGA VGS+ K
Sbjct: 201 SKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYKDGAPVGSIDK 260

Query: 679 GGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIG 706
           GGV+      +  S  FR +++  +  +V E   +G
Sbjct: 261 GGVKKQT--RQRGSLKFRINVTSFMKTVVDEFVNLG 285

BLAST of CmoCh01G012600.1 vs. ExPASy Swiss-Prot
Match: Q9ZVX1 (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 7.4e-82
Identity = 153/280 (54.64%), Postives = 194/280 (69.29%), Query Frame = 0

Query: 438  SFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEKDLPETIFVRVYESRMDL 497
            SF++FD   D  DH++       K + ++W KKV ++WKIL+ +LP+ IFVR YE RMDL
Sbjct: 823  SFRRFDISQDPLDHHFLGVD-GQKTKERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDL 882

Query: 498  MRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCGKVCLSLLN 557
            +RAVI+GA GTPY DGLFFFD   P++YP  PP  +YHSGG RLNPNLY  GKVCLSLLN
Sbjct: 883  LRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLN 942

Query: 558  TWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDKSQRYNEDT 617
            TW GRGNE W P  S++LQVLVS+QGL+LN+KPYFNE GY+ Q G+A GE  S  YNE+T
Sbjct: 943  TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENT 1002

Query: 618  FLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVK-GGVQDLD 677
            FLL+ KTM+Y +R+PPK FE+ ++ HF KRG+ IL AC AY  G  +GSL K   V D  
Sbjct: 1003 FLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMKGYLIGSLTKDASVIDER 1062

Query: 678  VGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQ 717
                S S  F+  L+K+   L   L ++G +DC +F   Q
Sbjct: 1063 SSANSTSVGFKLMLAKIAPKLFSALSEVG-ADCNEFQHLQ 1100

BLAST of CmoCh01G012600.1 vs. ExPASy TrEMBL
Match: A0A6J1FQP7 (uncharacterized protein LOC111446374 OS=Cucurbita moschata OX=3662 GN=LOC111446374 PE=4 SV=1)

HSP 1 Score: 1475.3 bits (3818), Expect = 0.0e+00
Identity = 721/721 (100.00%), Postives = 721/721 (100.00%), Query Frame = 0

Query: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60
           MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD
Sbjct: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60

Query: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL 120
           DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL
Sbjct: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL 120

Query: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180
           PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP
Sbjct: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180

Query: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240
           HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE
Sbjct: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240

Query: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ 300
           SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ
Sbjct: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ 300

Query: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI 360
           YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI
Sbjct: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI 360

Query: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST 420
           PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST
Sbjct: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST 420

Query: 421 SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480
           SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK
Sbjct: 421 SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480

Query: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540
           DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR
Sbjct: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540

Query: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600
           LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ
Sbjct: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600

Query: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660
           NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD
Sbjct: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660

Query: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720
           GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST
Sbjct: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720

Query: 721 W 722
           W
Sbjct: 721 W 721

BLAST of CmoCh01G012600.1 vs. ExPASy TrEMBL
Match: A0A6J1IZ24 (probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita maxima OX=3661 GN=LOC111480464 PE=4 SV=1)

HSP 1 Score: 1439.9 bits (3726), Expect = 0.0e+00
Identity = 700/721 (97.09%), Postives = 709/721 (98.34%), Query Frame = 0

Query: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60
           MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIIS+NVEVIDLD
Sbjct: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISVNVEVIDLD 60

Query: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL 120
           DDEASDADMAMFLQDNFD NNKGKGLYHHSDHQLKKTSSSCSF+PDVEV+ESTNKD SNL
Sbjct: 61  DDEASDADMAMFLQDNFDTNNKGKGLYHHSDHQLKKTSSSCSFIPDVEVVESTNKDGSNL 120

Query: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180
           PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIP+DIEVPIPWLP
Sbjct: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPTDIEVPIPWLP 180

Query: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240
           H QKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE
Sbjct: 181 HVQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240

Query: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ 300
            FEPFPSFSSQD QPN K+TASQYRDKSVSPYEVGHVNSLLGTH AK WWSDHLKCKKKQ
Sbjct: 241 GFEPFPSFSSQDIQPNMKSTASQYRDKSVSPYEVGHVNSLLGTHAAKPWWSDHLKCKKKQ 300

Query: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI 360
           YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHM++ KNMQPGQFI
Sbjct: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMEVVKNMQPGQFI 360

Query: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST 420
           PYGPEY KMKAKDPFFATHTASGLPFYPLG PPTLGEAVDMPWTQGSTQH+TNIAPDNST
Sbjct: 361 PYGPEYAKMKAKDPFFATHTASGLPFYPLGVPPTLGEAVDMPWTQGSTQHFTNIAPDNST 420

Query: 421 SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480
           SVSI PENLDAILANFESFKQFDTV+DHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK
Sbjct: 421 SVSIPPENLDAILANFESFKQFDTVNDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480

Query: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540
           DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR
Sbjct: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540

Query: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600
           LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ
Sbjct: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600

Query: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660
           NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD
Sbjct: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660

Query: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720
           GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST
Sbjct: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720

Query: 721 W 722
           W
Sbjct: 721 W 721

BLAST of CmoCh01G012600.1 vs. ExPASy TrEMBL
Match: A0A6J1FEJ0 (uncharacterized protein LOC111443352 OS=Cucurbita moschata OX=3662 GN=LOC111443352 PE=4 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.6e-271
Identity = 483/720 (67.08%), Postives = 566/720 (78.61%), Query Frame = 0

Query: 1   MEPPLSSNSFQNSKEQELS-VSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDL 60
           MEP   +NS  NSK+QE+S  SSSG  KPQVLG   +IK+PP MLKD+GI  L  + +D+
Sbjct: 1   MEPSFPNNSSINSKKQEVSGSSSSGSPKPQVLGTEPMIKRPPNMLKDQGINLL--DRVDV 60

Query: 61  DDDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESN 120
           D+DEAS  + A+F  D+FD N+KGKG+Y HSDHQLK+T +S S  PDVE+ ES N+DESN
Sbjct: 61  DEDEAS--NNAVFNHDDFDTNDKGKGIYQHSDHQLKETFASHSLSPDVEIQESPNEDESN 120

Query: 121 LPSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWL 180
            PSASHNF+++DN+ S+SS + ++W  FDE MDVDAYSALQAHFDHMDIP DIE PIPWL
Sbjct: 121 FPSASHNFVEIDNHFSDSSNDYEDWTAFDEFMDVDAYSALQAHFDHMDIPPDIEAPIPWL 180

Query: 181 PHGQKGNQTNIETS---SLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLS 240
           PHGQ GN  + ETS   S LAEPQ +P  VN LG++ A ++LK AL+NSL+L ADI V +
Sbjct: 181 PHGQNGNNIDTETSTSWSSLAEPQTEPGPVNHLGKSPAHVQLKEALSNSLNLQADIGVQN 240

Query: 241 HLHESFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKA-WWSDHLK 300
           HL E  EPF SF SQD QPN+K+  S+YR K    Y V H+NS LG   AK+  + D  K
Sbjct: 241 HLPEDIEPFASFPSQDVQPNRKSATSRYRGK----YGVSHLNSALGIDAAKSRSFLDPFK 300

Query: 301 CKKKQYGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQ 360
            KKK +  NN S N  DA+KLHNGGETS T DSVKK AG+H MP HS FPG+M+ FK+ +
Sbjct: 301 RKKKLHELNNISINTSDAIKLHNGGETSDTADSVKKLAGLHSMP-HSKFPGYMEFFKHPE 360

Query: 361 PGQFIPYGPEYTKMKAKDPFFATHTASGL--PFYPLGAPPTLGEAVDMPWTQGSTQHYTN 420
            GQ +P+ P+Y KMKAK+PFF  H ASGL  PF PL  PPTLGE VDMPW Q ST+   N
Sbjct: 361 AGQPMPHWPDYAKMKAKEPFFGPHMASGLYGPFSPL-VPPTLGEVVDMPWVQSSTKSLPN 420

Query: 421 IAPDNSTSVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQE 480
           I PD+S + +I+P +LD IL NFE FKQFDTVDDHSDH+Y+S GFSMKQ  KKWTKK+Q+
Sbjct: 421 IDPDDSINETIAPTDLDEILGNFEQFKQFDTVDDHSDHHYTSKGFSMKQASKKWTKKIQD 480

Query: 481 EWKILEKDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVH 540
           +WKIL+ DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPT YPD PP V+
Sbjct: 481 DWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTAYPDLPPHVY 540

Query: 541 YHSGGLRLNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFN 600
           YHS GLRLNPNLYNCGKVCLSLLNTW G GNE W+PGMSTMLQVLVSIQGLILNTKPYFN
Sbjct: 541 YHSKGLRLNPNLYNCGKVCLSLLNTWRGSGNENWVPGMSTMLQVLVSIQGLILNTKPYFN 600

Query: 601 EPGYEYQNGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILV 660
           EPGY YQ+GS  GE++SQRYNE+ ++LSIKTMV+NIRRPPKHFEDFVRGHFFKR HDILV
Sbjct: 601 EPGYAYQSGSVAGENRSQRYNEEAYILSIKTMVFNIRRPPKHFEDFVRGHFFKRAHDILV 660

Query: 661 ACKAYTDGAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFM 714
           ACKAY  GA+VGSLVK  V+DL   +KSCS HF+  ++ +LS LV   K+IGVSDCEKF+
Sbjct: 661 ACKAYIGGAQVGSLVKDRVKDLKPSNKSCSDHFKQCVNHVLSRLVETFKRIGVSDCEKFL 710

BLAST of CmoCh01G012600.1 vs. ExPASy TrEMBL
Match: A0A6J1IGV9 (uncharacterized protein LOC111476837 OS=Cucurbita maxima OX=3661 GN=LOC111476837 PE=4 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 7.5e-271
Identity = 482/720 (66.94%), Postives = 561/720 (77.92%), Query Frame = 0

Query: 1   MEPPLSSNSFQNSKEQELS-VSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDL 60
           MEP   +NS  NSK+QE+S  SSSG  KPQVLG   +IK+PP MLKD+GII L  + +D+
Sbjct: 1   MEPSFPNNSSNNSKKQEVSGSSSSGSPKPQVLGTEPMIKRPPNMLKDQGIILL--DRVDV 60

Query: 61  DDDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESN 120
           D+DEAS  D A+F  D+FD N+KGKG+YHHSDHQLK+T  S S  PDVE+ ES N+DESN
Sbjct: 61  DEDEAS--DNAVFNHDDFDTNDKGKGIYHHSDHQLKETFVSHSLSPDVEIQESPNEDESN 120

Query: 121 LPSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWL 180
            PSASHNF+++DN+ S+SS + ++W  FDE MDVDAYSALQAHFDHMDIP DIE PIPWL
Sbjct: 121 FPSASHNFVEIDNHFSDSSNDYEDWTAFDEFMDVDAYSALQAHFDHMDIPPDIEAPIPWL 180

Query: 181 PHGQKGNQTNIETS---SLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLS 240
           PHGQ GN  + ETS   S LAEPQ +P  VN LG++ A +++K AL+ SL+L ADI V +
Sbjct: 181 PHGQNGNNIDTETSSSWSSLAEPQSEPGPVNHLGKSPAHVQMKEALSKSLNLQADIGVQN 240

Query: 241 HLHESFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKA-WWSDHLK 300
           HL E  EPF SF SQD QPN+K+  S+YR K    Y V H+NS LG   AK+  + D  K
Sbjct: 241 HLPEDIEPFASFPSQDVQPNRKSATSRYRGK----YGVSHLNSALGIDAAKSRSFLDPFK 300

Query: 301 CKKKQYGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQ 360
            KKK +  NN S N  DAMK HNGGETS T DSVKK  G+  MP HS FPG++D FK+ +
Sbjct: 301 RKKKLHELNNISINTSDAMKFHNGGETSDTADSVKKLTGLQSMP-HSKFPGYVDFFKHPE 360

Query: 361 PGQFIPYGPEYTKMKAKDPFFATHTASGL--PFYPLGAPPTLGEAVDMPWTQGSTQHYTN 420
            GQ +PY P+Y KMKAK+PFF  H ASGL  PF PL  PPTLGE VDMPW Q ST+   N
Sbjct: 361 AGQPMPYWPDYAKMKAKEPFFGPHMASGLYGPFSPL-VPPTLGEVVDMPWVQSSTKSLPN 420

Query: 421 IAPDNSTSVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQE 480
           I PD+S S +I+P +LD IL NFE FKQFDTVDDHSDH+Y+S GFSMKQ  KKWTK +Q+
Sbjct: 421 IDPDDSISETIAPTDLDEILGNFERFKQFDTVDDHSDHHYTSKGFSMKQASKKWTKTIQD 480

Query: 481 EWKILEKDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVH 540
           +WKIL+ DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPT YPD PP V+
Sbjct: 481 DWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTAYPDLPPHVY 540

Query: 541 YHSGGLRLNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFN 600
           YHS GLRLNPNLYNCGKVCLSLLNTW G GNE W+PGMSTMLQVLVSIQGLILNTKPYFN
Sbjct: 541 YHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFN 600

Query: 601 EPGYEYQNGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILV 660
           EPGY YQ+GS  GE++SQRYNE+ ++LSIKTMV+NIRRPPKHFEDFVRGHFFKR HDILV
Sbjct: 601 EPGYAYQSGSVAGENRSQRYNEEAYILSIKTMVFNIRRPPKHFEDFVRGHFFKRAHDILV 660

Query: 661 ACKAYTDGAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFM 714
           ACKAY  GA+VGSLVK  V+D    +KSCS HF+  ++ +LS LV   K+IGVSDCEKF+
Sbjct: 661 ACKAYIGGAQVGSLVKDRVKDFKPSNKSCSDHFQQCVNHVLSRLVETFKRIGVSDCEKFL 710

BLAST of CmoCh01G012600.1 vs. ExPASy TrEMBL
Match: A0A6J1CEB1 (probable ubiquitin-conjugating enzyme E2 26 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010440 PE=4 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 2.4e-261
Identity = 472/725 (65.10%), Postives = 563/725 (77.66%), Query Frame = 0

Query: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60
           MEPP S NS QNSK + LS + SG MKPQVL  S +I +PPKMLK+KGI S  VEVID+D
Sbjct: 11  MEPPPSPNSSQNSKTKLLSGTGSGSMKPQVLRHSPLINRPPKMLKNKGIQS--VEVIDVD 70

Query: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL 120
            DE  D +  +++ D  + N+KGKG+YH+SDH+L++  +S S+ PDVE++E  N+ ES  
Sbjct: 71  KDE--DFNNVIYIHDGVENNDKGKGIYHNSDHELQEMMASPSYSPDVEILEGGNEVESAF 130

Query: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180
           PSA HN IDLDN+ S+SS   DEWIPFDE+MDVD YSALQAHFDHMDIP DIE PIPWLP
Sbjct: 131 PSAQHNVIDLDNHYSDSS-NGDEWIPFDEIMDVDEYSALQAHFDHMDIPPDIEAPIPWLP 190

Query: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240
           HGQ  +++   TSS  AE QI P +VNQLG + A  KLKA LANSL+L A+ID+++H  E
Sbjct: 191 HGQNRHKSIFGTSSSFAESQINPGSVNQLGNSPAHAKLKATLANSLNLQAEIDIVNHPPE 250

Query: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKA-WWSDHLKCKKK 300
             EPF SF+SQD  PNKK+ AS+YRDKS  P+EV  +NSLLG   AK  W+SD  K KKK
Sbjct: 251 GTEPFTSFTSQDVLPNKKSVASRYRDKSGLPHEV--INSLLGAEAAKTRWFSDPFKRKKK 310

Query: 301 QYGSNNSSFNQPDAMKLH-NGGETSA-TPDSVKKQAGIHKMPHHSNFPGHMD-IFKNMQP 360
            +GSNN S NQ D+ KLH NGGETS+ T ++VKKQ   + M    NFP +MD I+     
Sbjct: 311 LHGSNNISINQSDSRKLHNNGGETSSDTSETVKKQFSSNNMSSF-NFPAYMDNIYYPPGA 370

Query: 361 GQFIPYGPEYTKMKAKDPFFATHTASG--------LPFYPLGAPPTLGEAVDMPWTQGST 420
           G  +P+ P+Y KMK K+      + S         +P +PL  PPTLGE VDMPW QGST
Sbjct: 371 GASLPHWPDYAKMKTKELVLNPPSVSDYYGPFDHLIPPHPL-HPPTLGEVVDMPWIQGST 430

Query: 421 QHYTNIAPDNSTSVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWT 480
           +   N+APDNST  +IS  +LD I+  FE FK+FDTVDDHSDH+Y+S GF+MKQ PKKWT
Sbjct: 431 RDPANLAPDNSTLETISIADLDEIMRKFEQFKRFDTVDDHSDHHYTSKGFTMKQLPKKWT 490

Query: 481 KKVQEEWKILEKDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDA 540
           K++QEEWKILEKDLP+TIFVR YESRMDL+RAVIIGA+GTPYHDGLFFFDIFFP  YPD 
Sbjct: 491 KRIQEEWKILEKDLPDTIFVRAYESRMDLLRAVIIGAEGTPYHDGLFFFDIFFPMGYPDV 550

Query: 541 PPKVHYHSGGLRLNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNT 600
           PP V+YHSGGLRLNPNLYNCGKVCLSLLNTW G GNE+W+PGMSTMLQVLVSIQGLILNT
Sbjct: 551 PPLVYYHSGGLRLNPNLYNCGKVCLSLLNTWSGSGNERWIPGMSTMLQVLVSIQGLILNT 610

Query: 601 KPYFNEPGYEYQNGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRG 660
           KPYFNEPG+  QNGS  GE++SQ+YNE+T+LLS+KTMVYNIRRPPKHFEDFV+GHFFKR 
Sbjct: 611 KPYFNEPGFANQNGSVAGENRSQQYNEETYLLSMKTMVYNIRRPPKHFEDFVKGHFFKRA 670

Query: 661 HDILVACKAYTDGAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSD 714
           HDILV+CKAY DGA+VGSLVKGGVQDLD+ DK CS+HF+ SLS+ LSMLV   +++GV D
Sbjct: 671 HDILVSCKAYMDGAQVGSLVKGGVQDLDLSDKCCSNHFKQSLSQYLSMLVKTFQRMGVGD 726

BLAST of CmoCh01G012600.1 vs. NCBI nr
Match: XP_022940938.1 (uncharacterized protein LOC111446374 [Cucurbita moschata])

HSP 1 Score: 1475.3 bits (3818), Expect = 0.0e+00
Identity = 721/721 (100.00%), Postives = 721/721 (100.00%), Query Frame = 0

Query: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60
           MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD
Sbjct: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60

Query: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL 120
           DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL
Sbjct: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL 120

Query: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180
           PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP
Sbjct: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180

Query: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240
           HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE
Sbjct: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240

Query: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ 300
           SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ
Sbjct: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ 300

Query: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI 360
           YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI
Sbjct: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI 360

Query: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST 420
           PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST
Sbjct: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST 420

Query: 421 SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480
           SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK
Sbjct: 421 SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480

Query: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540
           DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR
Sbjct: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540

Query: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600
           LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ
Sbjct: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600

Query: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660
           NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD
Sbjct: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660

Query: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720
           GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST
Sbjct: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720

Query: 721 W 722
           W
Sbjct: 721 W 721

BLAST of CmoCh01G012600.1 vs. NCBI nr
Match: XP_023524739.1 (uncharacterized protein LOC111788588 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1470.3 bits (3805), Expect = 0.0e+00
Identity = 719/721 (99.72%), Postives = 720/721 (99.86%), Query Frame = 0

Query: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60
           MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD
Sbjct: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60

Query: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL 120
           DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSS SF+PDVEVIESTNKDESNL
Sbjct: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSRSFIPDVEVIESTNKDESNL 120

Query: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180
           PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP
Sbjct: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180

Query: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240
           HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE
Sbjct: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240

Query: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ 300
           SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ
Sbjct: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ 300

Query: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI 360
           YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI
Sbjct: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI 360

Query: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST 420
           PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST
Sbjct: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST 420

Query: 421 SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480
           SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK
Sbjct: 421 SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480

Query: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540
           DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR
Sbjct: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540

Query: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600
           LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ
Sbjct: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600

Query: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660
           NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD
Sbjct: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660

Query: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720
           GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST
Sbjct: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720

Query: 721 W 722
           W
Sbjct: 721 W 721

BLAST of CmoCh01G012600.1 vs. NCBI nr
Match: KAG6607877.1 (putative ubiquitin-conjugating enzyme E2 26, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1464.5 bits (3790), Expect = 0.0e+00
Identity = 716/721 (99.31%), Postives = 719/721 (99.72%), Query Frame = 0

Query: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60
           MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD
Sbjct: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60

Query: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL 120
           DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSS SF+PDVEVIESTNKDESNL
Sbjct: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSRSFIPDVEVIESTNKDESNL 120

Query: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180
           PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP
Sbjct: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180

Query: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240
           HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE
Sbjct: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240

Query: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ 300
           SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ
Sbjct: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ 300

Query: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI 360
           YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPH SNFPGHM+IFKNMQPGQFI
Sbjct: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHQSNFPGHMEIFKNMQPGQFI 360

Query: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST 420
           PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST
Sbjct: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST 420

Query: 421 SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480
           SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK
Sbjct: 421 SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480

Query: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540
           DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR
Sbjct: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540

Query: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600
           LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ
Sbjct: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600

Query: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660
           NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD
Sbjct: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660

Query: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720
           GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGV+DCEKFMPRQTPST
Sbjct: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVNDCEKFMPRQTPST 720

Query: 721 W 722
           W
Sbjct: 721 W 721

BLAST of CmoCh01G012600.1 vs. NCBI nr
Match: XP_022981285.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucurbita maxima])

HSP 1 Score: 1439.9 bits (3726), Expect = 0.0e+00
Identity = 700/721 (97.09%), Postives = 709/721 (98.34%), Query Frame = 0

Query: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLD 60
           MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIIS+NVEVIDLD
Sbjct: 1   MEPPLSSNSFQNSKEQELSVSSSGFMKPQVLGASSIIKQPPKMLKDKGIISVNVEVIDLD 60

Query: 61  DDEASDADMAMFLQDNFDANNKGKGLYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNL 120
           DDEASDADMAMFLQDNFD NNKGKGLYHHSDHQLKKTSSSCSF+PDVEV+ESTNKD SNL
Sbjct: 61  DDEASDADMAMFLQDNFDTNNKGKGLYHHSDHQLKKTSSSCSFIPDVEVVESTNKDGSNL 120

Query: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLP 180
           PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIP+DIEVPIPWLP
Sbjct: 121 PSASHNFIDLDNYCSESSAEDDEWIPFDEVMDVDAYSALQAHFDHMDIPTDIEVPIPWLP 180

Query: 181 HGQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240
           H QKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE
Sbjct: 181 HVQKGNQTNIETSSLLAEPQIKPDAVNQLGRASALLKLKAALANSLSLPADIDVLSHLHE 240

Query: 241 SFEPFPSFSSQDTQPNKKTTASQYRDKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQ 300
            FEPFPSFSSQD QPN K+TASQYRDKSVSPYEVGHVNSLLGTH AK WWSDHLKCKKKQ
Sbjct: 241 GFEPFPSFSSQDIQPNMKSTASQYRDKSVSPYEVGHVNSLLGTHAAKPWWSDHLKCKKKQ 300

Query: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFI 360
           YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHM++ KNMQPGQFI
Sbjct: 301 YGSNNSSFNQPDAMKLHNGGETSATPDSVKKQAGIHKMPHHSNFPGHMEVVKNMQPGQFI 360

Query: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNST 420
           PYGPEY KMKAKDPFFATHTASGLPFYPLG PPTLGEAVDMPWTQGSTQH+TNIAPDNST
Sbjct: 361 PYGPEYAKMKAKDPFFATHTASGLPFYPLGVPPTLGEAVDMPWTQGSTQHFTNIAPDNST 420

Query: 421 SVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480
           SVSI PENLDAILANFESFKQFDTV+DHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK
Sbjct: 421 SVSIPPENLDAILANFESFKQFDTVNDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEK 480

Query: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540
           DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR
Sbjct: 481 DLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLR 540

Query: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600
           LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ
Sbjct: 541 LNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQ 600

Query: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660
           NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD
Sbjct: 601 NGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTD 660

Query: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720
           GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST
Sbjct: 661 GAEVGSLVKGGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPST 720

Query: 721 W 722
           W
Sbjct: 721 W 721

BLAST of CmoCh01G012600.1 vs. NCBI nr
Match: KAG7037406.1 (putative ubiquitin-conjugating enzyme E2 26 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 691/700 (98.71%), Postives = 694/700 (99.14%), Query Frame = 0

Query: 26  MKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLDDDEASDADMAMFLQDNFDANNKGKG 85
           MKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLDDDEASDADMAMFLQDNFDANNKGKG
Sbjct: 1   MKPQVLGASSIIKQPPKMLKDKGIISLNVEVIDLDDDEASDADMAMFLQDNFDANNKGKG 60

Query: 86  LYHHSDHQLKKTSSSCSFVPDVEVIESTNKDESNLPSASHNFIDLDNYCSESSAEDDEWI 145
           LYHHSDHQLKKTSSS SF+PDVEVIESTNKDESNLPSASHNFIDLDNYCSESSAEDDEWI
Sbjct: 61  LYHHSDHQLKKTSSSRSFIPDVEVIESTNKDESNLPSASHNFIDLDNYCSESSAEDDEWI 120

Query: 146 PFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLPHGQKGNQTNIETSSLLAEPQIKPDA 205
           PFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLPHGQKGNQTNIETSSLLAEPQIKPDA
Sbjct: 121 PFDEVMDVDAYSALQAHFDHMDIPSDIEVPIPWLPHGQKGNQTNIETSSLLAEPQIKPDA 180

Query: 206 VNQLGRASALLKLKAALANSLSLPADIDVLSHLHESFEPFPSFSSQDTQPNKKTTASQYR 265
           VNQLGRASALLKLKAALANSLSLPADIDVLSHLHESFEPFPSFSSQDTQPNKKTTASQYR
Sbjct: 181 VNQLGRASALLKLKAALANSLSLPADIDVLSHLHESFEPFPSFSSQDTQPNKKTTASQYR 240

Query: 266 DKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQYGSNNS----SFNQPDAMKLHNGGE 325
           DKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQYGSNNS    SFNQPDAMKLHNGGE
Sbjct: 241 DKSVSPYEVGHVNSLLGTHVAKAWWSDHLKCKKKQYGSNNSSFNHSFNQPDAMKLHNGGE 300

Query: 326 TSATPDSVKKQAGIHKMPHHSNFPGHMDIFKNMQPGQFIPYGPEYTKMKAKDPFFATHTA 385
           TSATPDSVKKQAGIHKMPH SNFPGHM+IFKNMQPGQFIPYGPEYTKMKAKDPFFATHTA
Sbjct: 301 TSATPDSVKKQAGIHKMPHQSNFPGHMEIFKNMQPGQFIPYGPEYTKMKAKDPFFATHTA 360

Query: 386 SGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNSTSVSISPENLDAILANFESFKQ 445
           SGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNSTSVSISPENLDAILANFESFKQ
Sbjct: 361 SGLPFYPLGAPPTLGEAVDMPWTQGSTQHYTNIAPDNSTSVSISPENLDAILANFESFKQ 420

Query: 446 FDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEKDLPETIFVRVYESRMDLMRAV 505
           FDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEKDLPETIFVRVYESRMDLMRAV
Sbjct: 421 FDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEKDLPETIFVRVYESRMDLMRAV 480

Query: 506 IIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCGKVCLSLLNTWDG 565
           IIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCGKVCLSLLNTWDG
Sbjct: 481 IIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCGKVCLSLLNTWDG 540

Query: 566 RGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDKSQRYNEDTFLLS 625
           RGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDKSQRYNEDTFLLS
Sbjct: 541 RGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDKSQRYNEDTFLLS 600

Query: 626 IKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVKGGVQDLDVGDKS 685
           IKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVKGGVQDLDVGDKS
Sbjct: 601 IKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVKGGVQDLDVGDKS 660

Query: 686 CSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQTPSTW 722
           CSSHFRHSLSKLLSMLVVELKKIGV+DCEKFMPRQTPSTW
Sbjct: 661 CSSHFRHSLSKLLSMLVVELKKIGVNDCEKFMPRQTPSTW 700

BLAST of CmoCh01G012600.1 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 378.6 bits (971), Expect = 1.1e-104
Identity = 177/270 (65.56%), Postives = 209/270 (77.41%), Query Frame = 0

Query: 439 FKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEKDLPETIFVRVYESRMDLM 498
           FK+FDTV+D SDH+Y+S G S KQ  K W KKVQ +WKILE DLPE I VR  ESRMDL+
Sbjct: 244 FKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLL 303

Query: 499 RAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCGKVCLSLLNT 558
           RAVIIGA+GTPYHDGLFFFDI FP  YP  PP VHYHSGGLR+NPNLYNCGKVCLSLL T
Sbjct: 304 RAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGT 363

Query: 559 WDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDKSQRYNEDTF 618
           W G   EKWLP  STMLQ+LVSIQ LILN KPYFNEPGY    G+A GE KS+ Y+E+ F
Sbjct: 364 WAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSENVF 423

Query: 619 LLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVKGGVQDLDVG 678
           LLS+KTMVY+IRRPP+HFE++V+ H+F R HDI+ AC AY  GA +GS+VKGGVQDL+  
Sbjct: 424 LLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMVKGGVQDLEEA 483

Query: 679 DKSCSSHFRHSLSKLLSMLVVELKKIGVSD 709
            +S S  F+  ++  +  +V E  K+GV +
Sbjct: 484 RQSGSKKFKTDVASFMQTVVDEFVKLGVKE 513

BLAST of CmoCh01G012600.1 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 375.6 bits (963), Expect = 9.2e-104
Identity = 198/368 (53.80%), Postives = 241/368 (65.49%), Query Frame = 0

Query: 361 PYGPEYTKMKAKDPFFATHTASGLPFYPLG-AP-PTLGEA----VDMPWTQGSTQHYTNI 420
           PY   Y+ +K     F+ +     P   LG AP P  G +     + P  + +   +   
Sbjct: 224 PYAVHYSALKTA---FSNYLQPQTPDTVLGEAPAPAAGSSGLLPPNTPGFKSNAARFKEE 283

Query: 421 APDNSTSVSISPENLDAILANFESFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEE 480
            P      S    N++  L  +  FK+FD V+D SDH+Y+S G + KQ  K W K++Q+E
Sbjct: 284 PPILPPDDSRVKRNMEDYLGLYLFFKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDE 343

Query: 481 WKILEKDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHY 540
           W+ILEKDLPE IFVR YESRMDL+RAVIIGAQGTPYHDGLFFFDIFFP  YP  PP VHY
Sbjct: 344 WRILEKDLPEMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHY 403

Query: 541 HSGGLRLNPNLYNCGKVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNE 600
           HSGGLR+NPNLYNCGKVCLSLL TW G   EKW+P  STMLQVLVSIQGLILN KPYFNE
Sbjct: 404 HSGGLRINPNLYNCGKVCLSLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNE 463

Query: 601 PGYEYQNGSAMGEDKSQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVA 660
           PGYE   GSA GE  S+ Y+E+TF+LS+KTMVY +RRPPK+FEDF  GHFF   HD+L A
Sbjct: 464 PGYERSAGSAHGESTSKAYSENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKA 523

Query: 661 CKAYTDGAEVGSLVKGGVQDLDVGDKSCSS-HFRHSLSKLL-SMLVVELKKIGVSDCEKF 720
           C AY +GA  G LVK G  D++      SS  FR  ++  + ++L+ E   +GV   E  
Sbjct: 524 CNAYRNGATPGYLVK-GAPDVEENSAGMSSLKFRTDVATFVETVLLKEFILLGVLGLEPE 583

BLAST of CmoCh01G012600.1 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 329.7 bits (844), Expect = 5.8e-90
Identity = 161/276 (58.33%), Postives = 197/276 (71.38%), Query Frame = 0

Query: 439 FKQFDTVDDHSDHYYSSNG---------FSMKQEPKKWTKKVQEEWKILEKDLPETIFVR 498
           FK+FDTV+D SDH+Y++ G            K  PK W K +Q+EWKIL+K+LPETIFVR
Sbjct: 21  FKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKNLPETIFVR 80

Query: 499 VYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCG 558
             ESR+DL+RAVIIGA+GTPYHDGLFFFDI FP  YP  PPKVHYHSGGLR+NPNLY CG
Sbjct: 81  ACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGLRINPNLYKCG 140

Query: 559 KVCLSLLNTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDK 618
           KVCLSL++TW G+  EKWLP  STMLQ+LVSIQ LILN KPY+NEPGYE   G+ +GE  
Sbjct: 141 KVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSMGTPLGESY 200

Query: 619 SQRYNEDTFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVK 678
           S+ Y+E+ F+ S+KTM         HFE+FVR HFF R HDI+ AC AY DGA VGS+ K
Sbjct: 201 SKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYKDGAPVGSIDK 260

Query: 679 GGVQDLDVGDKSCSSHFRHSLSKLLSMLVVELKKIG 706
           GGV+      +  S  FR +++  +  +V E   +G
Sbjct: 261 GGVKKQT--RQRGSLKFRINVTSFMKTVVDEFVNLG 285

BLAST of CmoCh01G012600.1 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 306.6 bits (784), Expect = 5.3e-83
Identity = 153/280 (54.64%), Postives = 194/280 (69.29%), Query Frame = 0

Query: 438  SFKQFDTVDDHSDHYYSSNGFSMKQEPKKWTKKVQEEWKILEKDLPETIFVRVYESRMDL 497
            SF++FD   D  DH++       K + ++W KKV ++WKIL+ +LP+ IFVR YE RMDL
Sbjct: 823  SFRRFDISQDPLDHHFLGVD-GQKTKERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDL 882

Query: 498  MRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCGKVCLSLLN 557
            +RAVI+GA GTPY DGLFFFD   P++YP  PP  +YHSGG RLNPNLY  GKVCLSLLN
Sbjct: 883  LRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLN 942

Query: 558  TWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDKSQRYNEDT 617
            TW GRGNE W P  S++LQVLVS+QGL+LN+KPYFNE GY+ Q G+A GE  S  YNE+T
Sbjct: 943  TWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENT 1002

Query: 618  FLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVK-GGVQDLD 677
            FLL+ KTM+Y +R+PPK FE+ ++ HF KRG+ IL AC AY  G  +GSL K   V D  
Sbjct: 1003 FLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMKGYLIGSLTKDASVIDER 1062

Query: 678  VGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDCEKFMPRQ 717
                S S  F+  L+K+   L   L ++G +DC +F   Q
Sbjct: 1063 SSANSTSVGFKLMLAKIAPKLFSALSEVG-ADCNEFQHLQ 1100

BLAST of CmoCh01G012600.1 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 303.9 bits (777), Expect = 3.4e-82
Identity = 149/273 (54.58%), Postives = 184/273 (67.40%), Query Frame = 0

Query: 439 FKQFDTVDDHSDHYY--SSNGFSMKQEPKKWTKKVQEEWKILEKDLPETIFVRVYESRMD 498
           F+QFD V+D SDH++  S  G +  Q  K W KKVQ+EW  LE +LP TI+VRV E RMD
Sbjct: 633 FRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMD 692

Query: 499 LMRAVIIGAQGTPYHDGLFFFDIFFPTNYPDAPPKVHYHSGGLRLNPNLYNCGKVCLSLL 558
           L+RA ++GA GTPYHDGLFFFDI  P  YP  PP VHYHSGG+RLNPNLY  G+VCLSLL
Sbjct: 693 LLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLL 752

Query: 559 NTWDGRGNEKWLPGMSTMLQVLVSIQGLILNTKPYFNEPGYEYQNGSAMGEDKSQRYNED 618
           NTW G G E W  G S++LQ+L+S Q L+LN KPYFNE GY+ Q G A GE  S  YNE+
Sbjct: 753 NTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNEN 812

Query: 619 TFLLSIKTMVYNIRRPPKHFEDFVRGHFFKRGHDILVACKAYTDGAEVGSLVKGGVQDLD 678
            FL++ K+M+  +R+PPKHFE  V+ HF  R   +L ACKAY +G  VGS       +L 
Sbjct: 813 AFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGSSA-----NLQ 872

Query: 679 VGDKSCSSHFRHSLSKLLSMLVVELKKIGVSDC 710
               + S+ F+  LSKL   L+    +IGV DC
Sbjct: 873 GNSTTNSTGFKIMLSKLYPKLLEAFSEIGV-DC 899