Cmc12g0327461.1 (mRNA) Melon (Charmono) v1.1

Overview


Name	Cmc12g0327461.1
Type	mRNA
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	berberine bridge enzyme-like 18
Location	CMiso1.1chr12: 18402861 .. 18405518 (-)
Sequence length	2144
RNA-Seq Expression	Cmc12g0327461.1
Synteny	Cmc12g0327461.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTCTTTTTAGCTAAAGATACGATAGGATGGGATAACACAATAGTTACGTTGAAACATTTCAGTTTATTTGACACATCATATATAGCACTATGAATGGAGAAGGTTTCAATCACAAAGTTTTCCAACCTAACCATGATGCTTTCTCCGTTAGTCCCTCTTTCCCTCGTCTTCATTGTTGTATGTTCATCATCTTCTTTATGGGCAGCAACTTCTTCTTCTATAAAACATGAGGAATTTCTTCAATGTCTTCTTCATCATTCTCCTGATACTTCCTCCATTAACAAACTGGTTCACACTCCCATCAACTCCTCCTTTTCCACTCTCTTCAATTTCCCCACTCGAAACTCCAGATTCTCAACTCCCAACACCCCAAAACCACTCCTCATCATAACCCCATCAAATATTTCCCACATTCAAGCAGCCGTCATTTGCTCCAAATCTCACGGCCTTCAAATCCGAATCCGAAGCGGCGGTCACGACTTTGAGGGCCTTTCTTACGTAGCCTACCACAGATTCATCGTAGTCGATCTTATTAATCTAAGGTCGGTCACAGTTGATGTGGAACAAAGTACTGCATGGGTACAGTCCGGTGCAACTCTAGGTGAACTTTACTACAAAATTGGGAAGAAAAGTCGAACCTTGGGCTTCCCGGCGGGTATTTGTCCAACTGTTGGTGTTGGTGGGCACTTTAGTGGTGGTGGATATGGGTCTTTGTTAAGGAAATATGGTGTTGCAGCCGATAATGTAATAGACGCTTATTTGGTTGATGCAAATGGGGAGTTTCATGATAGAGAGTCGATGGGGGAGGATTTGTTTTGGGCCATTAGAGGCGGCGGCGGAGGAAGCTTTGGAGTTGTGGTGGCGTGGAAGGTCAAGCTGGTTCCGGTTCCAGCGTCCGTAACGATTTGCTCCGCCAAGAGAAGTGTGGAGGAAGAAGCAATTAAGCTAATAGATCGGTGGCAATATGTGGCAAACAAATTGGAAGAAGACCTATTTCTTGCCATCTTATGGAATGGTATTTTCACTTGACACTTTTTTTTTTTTTTTTTAATTTCTAATGAAAAGGAAACAAACCAAATGGTTTAAAAATTATAATGAAACACAGGCTGTTTTTAATTAGATTTAAAATGTTTTAACATTTTTATATCAATAAAATCTAAATTCTAAAAAAAGTATTTGAAAAAGATATTATATATTTATATTACTATATACATATGCATACTTACATATTTAGTTAGGCAAATAATGAGCGCATTAGATTGGGAGACCAAAATATGACCATTCAAACTTTGAGTTCTGATTGACATAGATTGAGCATAATTTGACTTAGGCCATACTTTTAGGTAAGTAGTAATTGATACTCTTGTTGTTGAGAGTACATATTTGGCATGGCTTTGTTGGAAACTACTTTTATTGGTGTTATCAGTTGAGGTGTGTTTGAGCATTATTTGTTATTGAGTTATTTTGGTGGTTTATTGACACACATTGATTTAAACTCAAATATATATATCGTTGTTGATGATGCATAGGTGGGAAAGTTTCAGCCCAAGGAGGAGGAAAAATTAATCCAATAGCTTTATTCTTCCCTTTGTTTCTTGGAAATGTAAATGAACTGATGGCAATCCTAAACAAAACATTTCCTGAATTGGGTTTCACAAAAGAAGAATGCAAAGAAAAAAGTTGGATTGAATCAGCTGCTTCTGCGGCCAATAGGTTTCAAATTGAAGATCAACCCTTGGAAGCTTTGCTTAATAGAACACCTATAAACCATGGAAGCAGCTTCAAACTCAAATCTGACTATGTTAAGGAGCCCATGACTAAAACTGCAATTGAGGGCATATGGAAAAGATTAGAATCTCAAGATATAGAGGGAGTAACCCTTGCACTTATTCCATATGGAGGGAGGATGAGTCGAATTTCCGAGTCAAATATTCCTTTCTCACATAGAGCTGGAATTCTATACCAAATTGGATACATTCTAGGGTGGGATGAAAAAGGTGTGGAAGCAGAGGAACGACACTTAAATTGGATAAGGGAGATTTATAGTTACATGACTCCTTTTGTTTCAAAATCTCCGAGAACTGCATATGTCAACTATAGAGACCTTGATATTGGATCAAACAATAAGTATGGAAAGATAAATTACAAGCAAGCCTGTGTTTTTGGTTCAAAATATTTTGGTAATAATTTTAATAGATTGGTAAAAGTGAAGACTAAGGTGGATCCTTACAATTTCTTCTGGCATGAACAAAGCATACCACTCTCTTCCAACCTTTAATATGGTTTGAACAATTTGGTATTTGGTTTCATGTTTACTCCTACACATATGCCCAACCTATTATGGTGACGAAATAAGGGATTTGTTTGGATTGCGTTTAACCATATATATAGTGACAAGATAAGGGATTTGTTTGGATTGCTTTTAACCATAGATATGGTGACAAGATAAAAGGATATGCATTAGATGCGAATGTGTTGCATTCACAATTAAATAATGGTAATTAATTCTTAGTTTGTCTCTTGTCGTTATATTATATCTAGCAGTATATTGTTTATGTTTATTTCTCCAGTTCATCAATCCTTTTGTCTTTGTAGTACTTTTAAGGTAATTTTTCAATCTTACCGTAGTGCGGTTAAAAACTTATACAATAAACTT

mRNA sequence

CTTTCTTTTTAGCTAAAGATACGATAGGATGGGATAACACAATAGTTACGTTGAAACATTTCAGTTTATTTGACACATCATATATAGCACTATGAATGGAGAAGGTTTCAATCACAAAGTTTTCCAACCTAACCATGATGCTTTCTCCGTTAGTCCCTCTTTCCCTCGTCTTCATTGTTGTATGTTCATCATCTTCTTTATGGGCAGCAACTTCTTCTTCTATAAAACATGAGGAATTTCTTCAATGTCTTCTTCATCATTCTCCTGATACTTCCTCCATTAACAAACTGGTTCACACTCCCATCAACTCCTCCTTTTCCACTCTCTTCAATTTCCCCACTCGAAACTCCAGATTCTCAACTCCCAACACCCCAAAACCACTCCTCATCATAACCCCATCAAATATTTCCCACATTCAAGCAGCCGTCATTTGCTCCAAATCTCACGGCCTTCAAATCCGAATCCGAAGCGGCGGTCACGACTTTGAGGGCCTTTCTTACGTAGCCTACCACAGATTCATCGTAGTCGATCTTATTAATCTAAGGTCGGTCACAGTTGATGTGGAACAAAGTACTGCATGGGTACAGTCCGGTGCAACTCTAGGTGAACTTTACTACAAAATTGGGAAGAAAAGTCGAACCTTGGGCTTCCCGGCGGGTATTTGTCCAACTGTTGGTGTTGGTGGGCACTTTAGTGGTGGTGGATATGGGTCTTTGTTAAGGAAATATGGTGTTGCAGCCGATAATGTAATAGACGCTTATTTGGTTGATGCAAATGGGGAGTTTCATGATAGAGAGTCGATGGGGGAGGATTTGTTTTGGGCCATTAGAGGCGGCGGCGGAGGAAGCTTTGGAGTTGTGGTGGCGTGGAAGGTCAAGCTGGTTCCGGTTCCAGCGTCCGTAACGATTTGCTCCGCCAAGAGAAGTGTGGAGGAAGAAGCAATTAAGCTAATAGATCGGTGGCAATATGTGGCAAACAAATTGGAAGAAGACCTATTTCTTGCCATCTTATGGAATGGTGGGAAAGTTTCAGCCCAAGGAGGAGGAAAAATTAATCCAATAGCTTTATTCTTCCCTTTGTTTCTTGGAAATGTAAATGAACTGATGGCAATCCTAAACAAAACATTTCCTGAATTGGGTTTCACAAAAGAAGAATGCAAAGAAAAAAGTTGGATTGAATCAGCTGCTTCTGCGGCCAATAGGTTTCAAATTGAAGATCAACCCTTGGAAGCTTTGCTTAATAGAACACCTATAAACCATGGAAGCAGCTTCAAACTCAAATCTGACTATGTTAAGGAGCCCATGACTAAAACTGCAATTGAGGGCATATGGAAAAGATTAGAATCTCAAGATATAGAGGGAGTAACCCTTGCACTTATTCCATATGGAGGGAGGATGAGTCGAATTTCCGAGTCAAATATTCCTTTCTCACATAGAGCTGGAATTCTATACCAAATTGGATACATTCTAGGGTGGGATGAAAAAGGTGTGGAAGCAGAGGAACGACACTTAAATTGGATAAGGGAGATTTATAGTTACATGACTCCTTTTGTTTCAAAATCTCCGAGAACTGCATATGTCAACTATAGAGACCTTGATATTGGATCAAACAATAAGTATGGAAAGATAAATTACAAGCAAGCCTGTGTTTTTGGTTCAAAATATTTTGGTAATAATTTTAATAGATTGGTAAAAGTGAAGACTAAGGTGGATCCTTACAATTTCTTCTGGCATGAACAAAGCATACCACTCTCTTCCAACCTTTAATATGGTTTGAACAATTTGGTATTTGGTTTCATGTTTACTCCTACACATATGCCCAACCTATTATGGTGACGAAATAAGGGATTTGTTTGGATTGCGTTTAACCATATATATAGTGACAAGATAAGGGATTTGTTTGGATTGCTTTTAACCATAGATATGGTGACAAGATAAAAGGATATGCATTAGATGCGAATGTGTTGCATTCACAATTAAATAATGGTAATTAATTCTTAGTTTGTCTCTTGTCGTTATATTATATCTAGCAGTATATTGTTTATGTTTATTTCTCCAGTTCATCAATCCTTTTGTCTTTGTAGTACTTTTAAGGTAATTTTTCAATCTTACCGTAGTGCGGTTAAAAACTTATACAATAAACTT

Coding sequence (CDS)

ATGGAGAAGGTTTCAATCACAAAGTTTTCCAACCTAACCATGATGCTTTCTCCGTTAGTCCCTCTTTCCCTCGTCTTCATTGTTGTATGTTCATCATCTTCTTTATGGGCAGCAACTTCTTCTTCTATAAAACATGAGGAATTTCTTCAATGTCTTCTTCATCATTCTCCTGATACTTCCTCCATTAACAAACTGGTTCACACTCCCATCAACTCCTCCTTTTCCACTCTCTTCAATTTCCCCACTCGAAACTCCAGATTCTCAACTCCCAACACCCCAAAACCACTCCTCATCATAACCCCATCAAATATTTCCCACATTCAAGCAGCCGTCATTTGCTCCAAATCTCACGGCCTTCAAATCCGAATCCGAAGCGGCGGTCACGACTTTGAGGGCCTTTCTTACGTAGCCTACCACAGATTCATCGTAGTCGATCTTATTAATCTAAGGTCGGTCACAGTTGATGTGGAACAAAGTACTGCATGGGTACAGTCCGGTGCAACTCTAGGTGAACTTTACTACAAAATTGGGAAGAAAAGTCGAACCTTGGGCTTCCCGGCGGGTATTTGTCCAACTGTTGGTGTTGGTGGGCACTTTAGTGGTGGTGGATATGGGTCTTTGTTAAGGAAATATGGTGTTGCAGCCGATAATGTAATAGACGCTTATTTGGTTGATGCAAATGGGGAGTTTCATGATAGAGAGTCGATGGGGGAGGATTTGTTTTGGGCCATTAGAGGCGGCGGCGGAGGAAGCTTTGGAGTTGTGGTGGCGTGGAAGGTCAAGCTGGTTCCGGTTCCAGCGTCCGTAACGATTTGCTCCGCCAAGAGAAGTGTGGAGGAAGAAGCAATTAAGCTAATAGATCGGTGGCAATATGTGGCAAACAAATTGGAAGAAGACCTATTTCTTGCCATCTTATGGAATGGTGGGAAAGTTTCAGCCCAAGGAGGAGGAAAAATTAATCCAATAGCTTTATTCTTCCCTTTGTTTCTTGGAAATGTAAATGAACTGATGGCAATCCTAAACAAAACATTTCCTGAATTGGGTTTCACAAAAGAAGAATGCAAAGAAAAAAGTTGGATTGAATCAGCTGCTTCTGCGGCCAATAGGTTTCAAATTGAAGATCAACCCTTGGAAGCTTTGCTTAATAGAACACCTATAAACCATGGAAGCAGCTTCAAACTCAAATCTGACTATGTTAAGGAGCCCATGACTAAAACTGCAATTGAGGGCATATGGAAAAGATTAGAATCTCAAGATATAGAGGGAGTAACCCTTGCACTTATTCCATATGGAGGGAGGATGAGTCGAATTTCCGAGTCAAATATTCCTTTCTCACATAGAGCTGGAATTCTATACCAAATTGGATACATTCTAGGGTGGGATGAAAAAGGTGTGGAAGCAGAGGAACGACACTTAAATTGGATAAGGGAGATTTATAGTTACATGACTCCTTTTGTTTCAAAATCTCCGAGAACTGCATATGTCAACTATAGAGACCTTGATATTGGATCAAACAATAAGTATGGAAAGATAAATTACAAGCAAGCCTGTGTTTTTGGTTCAAAATATTTTGGTAATAATTTTAATAGATTGGTAAAAGTGAAGACTAAGGTGGATCCTTACAATTTCTTCTGGCATGAACAAAGCATACCACTCTCTTCCAACCTTTAA

Protein sequence

MEKVSITKFSNLTMMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSAQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLSSNL

Homology

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BLAST of Cmc12g0327461.1 vs. NCBI nr
Match: XP_008445721.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0


Query: 1   MEKVSITKFSNLTMMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTS 60
           MEKVSITKFSNLTMMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTS
Sbjct: 1   MEKVSITKFSNLTMMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTS 60

Query: 61  SINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQ 120
           SINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQ
Sbjct: 61  SINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQ 120

Query: 121 IRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKS 180
           IRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKS
Sbjct: 121 IRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKS 180

Query: 181 RTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDL 240
           RTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDL
Sbjct: 181 RTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDL 240

Query: 241 FWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDL 300
           FWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDL
Sbjct: 241 FWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDL 300

Query: 301 FLAILWNGGKVSAQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWI 360
           FLAILWNGGKVSAQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWI
Sbjct: 301 FLAILWNGGKVSAQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWI 360

Query: 361 ESAASAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDI 420
           ESAASAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDI
Sbjct: 361 ESAASAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDI 420

Query: 421 EGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYS 480
           EGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYS
Sbjct: 421 EGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYS 480

Query: 481 YMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDP 540
           YMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDP
Sbjct: 481 YMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDP 540

Query: 541 YNFFWHEQSIPLSSNL 557
           YNFFWHEQSIPLSSNL
Sbjct: 541 YNFFWHEQSIPLSSNL 556

[Close]

BLAST of Cmc12g0327461.1 vs. NCBI nr
Match: KAA0047096.1 (tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05062.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1036.9 bits (2680), Expect = 6.1e-299
Identity = 520/543 (95.76%), Postives = 520/543 (95.76%), Query Frame = 0


Query: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73
           MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS
Sbjct: 1   MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 60

Query: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133
           FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193
           SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313
           VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA
Sbjct: 241 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 300

Query: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373
           QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 360

Query: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433
           DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR
Sbjct: 361 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 420

Query: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493
           MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA
Sbjct: 421 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 480

Query: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553
           YVNYRDLDIGSNNK                       LVKVKTKVDPYNFFWHEQSIPLS
Sbjct: 481 YVNYRDLDIGSNNK-----------------------LVKVKTKVDPYNFFWHEQSIPLS 520

Query: 554 SNL 557
           SNL
Sbjct: 541 SNL 520

[Close]

BLAST of Cmc12g0327461.1 vs. NCBI nr
Match: XP_011659211.2 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653319.1 hypothetical protein Csa_023346 [Cucumis sativus])

HSP 1 Score: 1024.2 bits (2647), Expect = 4.1e-295
Identity = 500/542 (92.25%), Postives = 522/542 (96.31%), Query Frame = 0


Query: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73
           M  SPL+PLSLVF+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60

Query: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133
           FS+L NFPTRNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120

Query: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193
           SYVAYH+FIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEE+LFLAI WNGGKVSA
Sbjct: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300

Query: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360

Query: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433
           DQPLEALLNRTPI HG SFKLKSDYVKEPMTK AI+GIWKRLESQDIEGVTLA+IPYGGR
Sbjct: 361 DQPLEALLNRTPITHG-SFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420

Query: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493
           M++ISES IPF HRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYMTPFVSKSPR A
Sbjct: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480

Query: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553
           YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVK+ VDPYNFFWHEQSIPLS
Sbjct: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540

Query: 554 SN 556
           S+
Sbjct: 541 SD 541

[Close]

BLAST of Cmc12g0327461.1 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 902.1 bits (2330), Expect = 2.3e-258
Identity = 444/543 (81.77%), Postives = 493/543 (90.79%), Query Frame = 0


Query: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSS-IKHEEFLQCLLHHSPDTSSINKLVHTPINS 73
           M  SPL+ LSL FI+VCSSSSL +ATSSS IKHEEFLQCLLHHSP+T SI KLV+ PINS
Sbjct: 1   MKFSPLILLSLAFIIVCSSSSLLSATSSSYIKHEEFLQCLLHHSPNTYSITKLVYAPINS 60

Query: 74  SFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEG 133
           S+S+L NFP+RNSRFSTPN P PLLIITP+NISHIQAA+ICSKSHGLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFPSRNSRFSTPNIPNPLLIITPTNISHIQAAIICSKSHGLQIRIRSGGHDFEG 120

Query: 134 LSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPT 193
           LSYVAY  FIVVDLINLRSVTVD E+S+AWVQSGATLGELYY+I +KS+TL FPAG+CPT
Sbjct: 121 LSYVAYVPFIVVDLINLRSVTVDAEKSSAWVQSGATLGELYYRIAEKSQTLAFPAGVCPT 180

Query: 194 VGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 253
           VGVGGHFSGGGYG LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGG+F
Sbjct: 181 VGVGGHFSGGGYGILLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGNF 240

Query: 254 GVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVS 313
           GVVVAWKVKLVPVP +VTIC+AKR++EE AI+LID+WQYV NKLEEDLFL+I  NGGK S
Sbjct: 241 GVVVAWKVKLVPVPETVTICAAKRTLEEGAIELIDQWQYVGNKLEEDLFLSIFLNGGKNS 300

Query: 314 AQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQI 373
           AQGGGK+NP ALFF LFLG  +EL+AIL K FPELG TKE+CKEKSWI SA SAANRFQI
Sbjct: 301 AQGGGKVNPTALFFSLFLGKADELVAILKKKFPELGLTKEDCKEKSWIGSAVSAANRFQI 360

Query: 374 EDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGG 433
           EDQPLEALLNRTP+ +G S K+KSDYVKE +TK  IEGIW+RL+SQDIEGVTLALIPYGG
Sbjct: 361 EDQPLEALLNRTPLANG-SLKIKSDYVKELITKVGIEGIWERLKSQDIEGVTLALIPYGG 420

Query: 434 RMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRT 493
           RM +IS+S IPFSHRAGI YQIGYI+GW E+G++AE+RH+NWIREIYSYMTPFVSKSPR 
Sbjct: 421 RMGQISDSEIPFSHRAGIRYQIGYIVGWKEQGLDAEKRHINWIREIYSYMTPFVSKSPRA 480

Query: 494 AYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPL 553
           AYVNYRD DIGSNNKYGK++YK+A V+G KYFGNNFNRLV++KTKVDP NFF HEQSIPL
Sbjct: 481 AYVNYRDHDIGSNNKYGKVSYKRARVWGLKYFGNNFNRLVRLKTKVDPSNFFRHEQSIPL 540

Query: 554 SSN 556
           S N
Sbjct: 541 SPN 542

[Close]

BLAST of Cmc12g0327461.1 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 876.3 bits (2263), Expect = 1.4e-250
Identity = 430/539 (79.78%), Postives = 478/539 (88.68%), Query Frame = 0


Query: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSS-IKHEEFLQCLLHHSPDTSSINKLVHTPINS 73
           M  SPL+PLSL FI++CSSSSL +ATSSS IK+EEFLQCLLHHSP+T SI  LV+ P NS
Sbjct: 1   MKFSPLIPLSLAFIILCSSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNS 60

Query: 74  SFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEG 133
           S+S+L NF  RN RFSTP  PKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120

Query: 134 LSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPT 193
           LSYVAYH FIVVDLINL SV VDVEQSTAWVQSGATLGELYY+I +KSRTL FPAG CPT
Sbjct: 121 LSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPT 180

Query: 194 VGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 253
           VGVGGHFSGGGYG+LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 254 GVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVS 313
           G+VVAWKVKLVPVPA+VTIC+A R+ +E AIKLID+WQYVANKL+EDLFL+I+  GGK+S
Sbjct: 241 GIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKIS 300

Query: 314 AQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQI 373
           AQ GGKINP ALFF LFLG  +EL+ IL   FPEL   KE+CKE SWIESA   AN  QI
Sbjct: 301 AQ-GGKINPTALFFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQI 360

Query: 374 EDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGG 433
           +DQPLEA LNRTP+ +G SFK+KSDYVKEP+TK AIEGIW+RL+SQDIEGV L LIPYGG
Sbjct: 361 DDQPLEAFLNRTPLANG-SFKIKSDYVKEPITKAAIEGIWQRLKSQDIEGVNLGLIPYGG 420

Query: 434 RMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRT 493
           RMS+ISES IPF HR G LY+IGYI+GW+E+GV+AE+RHLNWIREIYSYMTPFVSKSPR 
Sbjct: 421 RMSQISESEIPFPHRIGNLYKIGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSKSPRA 480

Query: 494 AYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIP 552
           AYVNYRDLDIGSN+KYGK +YK+AC++G KYFGNNFNRLV VK +VDPY+FF HEQSIP
Sbjct: 481 AYVNYRDLDIGSNDKYGKTSYKRACIWGKKYFGNNFNRLVHVKNEVDPYDFFRHEQSIP 537

[Close]

BLAST of Cmc12g0327461.1 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.7e-148
Identity = 259/510 (50.78%), Postives = 354/510 (69.41%), Query Frame = 0


Query: 46  EEFLQCLLHHSPDTSSINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNIS 105
           E FL+C   H P+  +  KLV+T  +  + ++ N   +N RF +  TPKPL+I+TPSN S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 106 HIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQS 165
           HIQA ++CSK  GLQIR RSGGHD EG+SY++   F+VVDL N+ S+ +DV   TAWV++
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 166 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 225
           GATLGE+YY I +K+  L FP G CPTVGVGGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 226 ANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVE-EEAIK 285
            +G+  DR+SMGEDLFWAIRGGGG +FG++ AWK+KLV VP+  TI S K+++E    +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 286 LIDRWQYVANKLEEDLFLAILWNGGKVS-AQGGGKINPIALFFPLFLGNVNELMAILNKT 345
           L ++WQ +A K ++DL L   +    ++   G  K      F  +F G V+ L+ ++NK+
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 346 FPELGFTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKE 405
           FPELG  K +CKE SWI++    S    F   +   E LL+R+     ++F +K DYVK+
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRS-AGKKTAFSIKLDYVKK 391

Query: 406 PMTKTAIEGIWKRLESQDIEGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWD 465
           P+ +TA+  I ++L  +D+      L PYGG M  ISES IPF HRAGI+Y++ Y   W+
Sbjct: 392 PIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWE 451

Query: 466 EKGVEAEERHLNWIREIYSYMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGS 525
           ++  E  E+H+NW+R +Y++ TP+VS++PR AY+NYRDLD+G  N     NY QA ++G 
Sbjct: 452 KQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGE 511

Query: 526 KYFGNNFNRLVKVKTKVDPYNFFWHEQSIP 552
           KYFG NFNRLVKVKTKVDP NFF +EQSIP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPNNFFRNEQSIP 538

[Close]

BLAST of Cmc12g0327461.1 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.5e-146
Identity = 265/539 (49.17%), Postives = 366/539 (67.90%), Query Frame = 0


Query: 30  CSSSSLW-------------AATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSSFST 89
           CS+ S W               TS +   E FL+C   + P+ ++  KLV+T  N  + +
Sbjct: 3   CSTFSFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMS 62

Query: 90  LFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYV 149
           + N    N RF++  TPKPL+I+TPS++SHIQ  ++CSK  GLQIR RSGGHD EG+SY+
Sbjct: 63  VLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYI 122

Query: 150 AYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVG 209
           +   F++VDL N+RS+ +DV   TAWV++GATLGE+YY + +K+  L   AG CPTV  G
Sbjct: 123 SQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAG 182

Query: 210 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVV 269
           GHF GGGYG L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWA+RGGG  SFG++V
Sbjct: 183 GHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIV 242

Query: 270 AWKVKLVPVPASVTICSAKRSVE-EEAIKLIDRWQYVANKLEEDLFLAILWNGGKVS-AQ 329
           AWK++LV VP S T+ S K+ +E  E +KL+++WQ +A K ++DL L   +    ++  Q
Sbjct: 243 AWKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQ 302

Query: 330 GGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAA--SAANRFQI 389
           G  K      F  +FLG V+ L+ ++NK+FPELG  K +C++ SWI++    S    +  
Sbjct: 303 GKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDT 362

Query: 390 EDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGG 449
           ++   E LL+R+   +G +FK+K DYVK+P+ ++    I ++L  +DI     AL PYGG
Sbjct: 363 DNFNKEILLDRSAGQNG-AFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGG 422

Query: 450 RMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRT 509
            M  ISES IPF HRAGILY++ YI  W+++  E  E+HLNWIR IY++MTP+VSK+PR 
Sbjct: 423 IMDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRL 482

Query: 510 AYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIP 552
           AY+NYRDLDIG N+     NY QA ++G KYFG NF+RLVKVKT VDP NFF +EQSIP
Sbjct: 483 AYLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIP 537

[Close]

BLAST of Cmc12g0327461.1 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.9e-146
Identity = 257/510 (50.39%), Postives = 354/510 (69.41%), Query Frame = 0


Query: 46  EEFLQCLLHHSPDTSSINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNIS 105
           E FL+CL  + P   +  KLV+T  +  + ++ N   +N RF++  TPKPL+I TP N+S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 106 HIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQS 165
           HIQ  ++CSK  GLQIR RSGGHD EG+SY++   F++VDL N+ SV +DV   TAWV+S
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 166 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 225
           GATLGE+YY I + +  L FPAG CPTVG GGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 226 ANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVE-EEAIK 285
            +G+  DR+SMGEDLFWAIRGGGG +FG++ AWK++LV VP+  TI S K+++E  E +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 286 LIDRWQYVANKLEEDLFLAILWNGGKVS-AQGGGKINPIALFFPLFLGNVNELMAILNKT 345
           L+++WQ +A   E++L L   +    ++  QG  K    + F  +F G V+ L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 346 FPELGFTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKE 405
           FPELG  K +CK+ SWI++    S    +   +   E LL+R+     ++F +K DYVK+
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSG-GRKAAFSIKLDYVKK 391

Query: 406 PMTKTAIEGIWKRLESQDIEGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWD 465
           P+ +TA+  I ++L  +D+        PYGG M  ISES IPF HRAGI Y+I YI  W+
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWE 451

Query: 466 EKGVEAEERHLNWIREIYSYMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGS 525
           ++  E  E+H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     NY QA ++G 
Sbjct: 452 KQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 526 KYFGNNFNRLVKVKTKVDPYNFFWHEQSIP 552
           KYFG NFNRLVKVKTKVDP NFF +EQSIP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

[Close]

BLAST of Cmc12g0327461.1 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 5.6e-146
Identity = 256/510 (50.20%), Postives = 354/510 (69.41%), Query Frame = 0


Query: 46  EEFLQCLLHHSPDTSSINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNIS 105
           E FL+C   + P   +  KLV+T  +  + ++ N   +N RF++  TPKPL+IITP N+S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 106 HIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQS 165
           HIQ  ++CSK  GLQIR RSGGHD EG+SY++   F++VDL N+ SV +DV   TAWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 166 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 225
           GATLGE+YY I + +  L FPAG CPTVG GGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 226 ANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVE-EEAIK 285
            +G+  DR+SMGEDLFWAIRGGGG +FG++ AWK++LV VP+  TI S K+++E  E +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 286 LIDRWQYVANKLEEDLFLAILWNGGKVS-AQGGGKINPIALFFPLFLGNVNELMAILNKT 345
           L+++WQ +A   E++L L   +    ++  QG  K    + F  +F G V+ L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 346 FPELGFTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKE 405
           FPELG  K +CK+ SWI++    S    +       E LL+R+     ++F +K DYVK+
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSG-GRKAAFSIKLDYVKK 391

Query: 406 PMTKTAIEGIWKRLESQDIEGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWD 465
           P+ +TA+  I ++L  +D+        PYGG M  ISES IPF HRAGI+Y+I YI  W+
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWE 451

Query: 466 EKGVEAEERHLNWIREIYSYMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGS 525
           ++  E  E+H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     NY QA ++G 
Sbjct: 452 KQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 526 KYFGNNFNRLVKVKTKVDPYNFFWHEQSIP 552
           KYFG NFNRLVKVKTKVDP NFF +EQSIP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

[Close]

BLAST of Cmc12g0327461.1 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 3.6e-145
Identity = 252/505 (49.90%), Postives = 355/505 (70.30%), Query Frame = 0


Query: 48  FLQCLLHHSPDTSSINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHI 107
           FLQCL     D++ ++K++HTP ++SFS++     +N RFS P+ PKP+LI+TP   S +
Sbjct: 37  FLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDV 96

Query: 108 QAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGA 167
           Q+AV C++  G+ IR RSGGHD+EGLSYV +  F+++DL NLRS+TVDV+  + WVQ+GA
Sbjct: 97  QSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGA 156

Query: 168 TLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDAN 227
           T+GELYY+IGKK+RTL FPAG+CPTVGVGGHFSGGGYG+LLRK+G+AAD+VIDA +VDA 
Sbjct: 157 TIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDAR 216

Query: 228 GEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLID 287
           G   +R  MGED FWAIRGGGG SF VV++WK+ L+ VP++VT+ +  +  E+ A+K+I 
Sbjct: 217 GRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIH 276

Query: 288 RWQYVANKLEEDLFLAILWNGGKVSAQGGGKINPIALFFP-LFLGNVNELMAILNKTFPE 347
           RWQ+VA+K+ +DLF+ ++    K         N +   FP L+LG+V  L+ ++NK FPE
Sbjct: 277 RWQFVADKVSDDLFIRVMLQRYK---------NMVRASFPGLYLGSVKNLLKMVNKEFPE 336

Query: 348 LGFTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKT 407
           LG  +++C E SWIES    A   ++ ++P+  L  RT      +FK KSD+V+EPM KT
Sbjct: 337 LGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRT--RASLAFKAKSDFVQEPMPKT 396

Query: 408 AIEGIWKRLESQDIEGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVE 467
           AI  +W+RL+  + E   L   P+GG+MS I++   PF HR G +Y+I Y+  W  +G +
Sbjct: 397 AISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW--RG-D 456

Query: 468 AEERHLNWIREIYSYMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGN 527
            +E+++ W+  +Y  M+ FV+KSPR AY+N RDLD+G      +  Y++   +G KYF N
Sbjct: 457 VKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKN 516

Query: 528 NFNRLVKVKTKVDPYNFFWHEQSIP 552
           NF RLV+VKT VDP +FF  EQSIP
Sbjct: 517 NFERLVRVKTSVDPSDFFCDEQSIP 524

[Close]

BLAST of Cmc12g0327461.1 vs. ExPASy TrEMBL
Match: A0A1S3BDD9 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0


Query: 1   MEKVSITKFSNLTMMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTS 60
           MEKVSITKFSNLTMMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTS
Sbjct: 1   MEKVSITKFSNLTMMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTS 60

Query: 61  SINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQ 120
           SINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQ
Sbjct: 61  SINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQ 120

Query: 121 IRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKS 180
           IRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKS
Sbjct: 121 IRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKS 180

Query: 181 RTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDL 240
           RTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDL
Sbjct: 181 RTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDL 240

Query: 241 FWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDL 300
           FWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDL
Sbjct: 241 FWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDL 300

Query: 301 FLAILWNGGKVSAQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWI 360
           FLAILWNGGKVSAQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWI
Sbjct: 301 FLAILWNGGKVSAQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWI 360

Query: 361 ESAASAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDI 420
           ESAASAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDI
Sbjct: 361 ESAASAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDI 420

Query: 421 EGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYS 480
           EGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYS
Sbjct: 421 EGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYS 480

Query: 481 YMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDP 540
           YMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDP
Sbjct: 481 YMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDP 540

Query: 541 YNFFWHEQSIPLSSNL 557
           YNFFWHEQSIPLSSNL
Sbjct: 541 YNFFWHEQSIPLSSNL 556

[Close]

BLAST of Cmc12g0327461.1 vs. ExPASy TrEMBL
Match: A0A5D3C1K3 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00270 PE=3 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 3.0e-299
Identity = 520/543 (95.76%), Postives = 520/543 (95.76%), Query Frame = 0


Query: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73
           MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS
Sbjct: 1   MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 60

Query: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133
           FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193
           SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313
           VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA
Sbjct: 241 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 300

Query: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373
           QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 360

Query: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433
           DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR
Sbjct: 361 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 420

Query: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493
           MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA
Sbjct: 421 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 480

Query: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553
           YVNYRDLDIGSNNK                       LVKVKTKVDPYNFFWHEQSIPLS
Sbjct: 481 YVNYRDLDIGSNNK-----------------------LVKVKTKVDPYNFFWHEQSIPLS 520

Query: 554 SNL 557
           SNL
Sbjct: 541 SNL 520

[Close]

BLAST of Cmc12g0327461.1 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.4e-245
Identity = 417/539 (77.37%), Postives = 473/539 (87.76%), Query Frame = 0


Query: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73
           M  S L+PLSL FIVVCSSSS+W + SS + HEEFL CLLHHSPD+ SI KLV+TPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133
           +S + NF  RN RFSTPNTPKPLLIITPSNISH+QAAVICSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193
           SYVAYH F+VVDLINL SVT++VEQSTAWVQSGATLGELYY+I +KSRTL FPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253
           GVGGHFSGGG+G+LLRKYG+AADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313
           +VVAWKVKLVPVPA+VTIC+  R++EEEAIKLID+WQYVANKL+EDLFL I   GGK+ A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373
           Q G KINPIALFF LFLG  +ELMAILNKTFP+LG TKEECKE SWIES     N  QIE
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433
           DQPLE LLNRTP+ +G + K+KSDYVKEP++K  I+ +W+RL+SQDIEG  L  IPYGGR
Sbjct: 361 DQPLEVLLNRTPLANG-NIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGR 420

Query: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493
           MS+ISES IPFSHRAG LY+IGY+ GW E G+ AE+ HLNWIR+IYSYMTPFV+KSPR A
Sbjct: 421 MSQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAA 480

Query: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPL 553
           YVNYRDLDIGSN+KYGK +YK+A V+GSKYFG+NF+RLV VK +VDPYNFF HEQSIP+
Sbjct: 481 YVNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPV 537

[Close]

BLAST of Cmc12g0327461.1 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.4e-245
Identity = 417/539 (77.37%), Postives = 473/539 (87.76%), Query Frame = 0


Query: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73
           M  S L+PLSL FIVVCSSSS+W + SS + HEEFL CLLHHSPD+ SI KLV+TPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133
           +S + NF  RN RFSTPNTPKPLLIITPSNISH+QAAVICSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193
           SYVAYH F+VVDLINL SVT++VEQSTAWVQSGATLGELYY+I +KSRTL FPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253
           GVGGHFSGGG+G+LLRKYG+AADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313
           +VVAWKVKLVPVPA+VTIC+  R++EEEAIKLID+WQYVANKL+EDLFL I   GGK+ A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373
           Q G KINPIALFF LFLG  +ELMAILNKTFP+LG TKEECKE SWIES     N  QIE
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433
           DQPLE LLNRTP+ +G + K+KSDYVKEP++K  I+ +W+RL+SQDIEG  L  IPYGGR
Sbjct: 361 DQPLEVLLNRTPLANG-NIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGR 420

Query: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493
           MS+ISES IPFSHRAG LY+IGY+ GW E G+ AE+ HLNWIR+IYSYMTPFV+KSPR A
Sbjct: 421 MSQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAA 480

Query: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPL 553
           YVNYRDLDIGSN+KYGK +YK+A V+GSKYFG+NF+RLV VK +VDPYNFF HEQSIP+
Sbjct: 481 YVNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPV 537

[Close]

BLAST of Cmc12g0327461.1 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.4e-224
Identity = 385/537 (71.69%), Postives = 455/537 (84.73%), Query Frame = 0


Query: 17  SPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTS--SINKLVHTPINSSF 76
           SPL+ LSL FI + SSSSLWA+   + KHE FLQCLL HS + S  SI+K++HTPINSS+
Sbjct: 5   SPLISLSLAFIFL-SSSSLWASAFPN-KHESFLQCLLDHSLEYSNYSISKVIHTPINSSY 64

Query: 77  STLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLS 136
           S++ +F  RN RFST  TPKPLLI+TPS++SHIQAAVICSKS+G QIR RSGGHDFEGLS
Sbjct: 65  SSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLS 124

Query: 137 YVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVG 196
           YVA+  FI+VDLINLRS+++D++ +TAWVQSGATLGELYY I +KSRTL FPAGICPTVG
Sbjct: 125 YVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVG 184

Query: 197 VGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGV 256
           VGGHFSGGGYG LLRKYG+AADNVIDAYLVDA+G+FH+RESMGEDLFWAIRGGGGGSFG+
Sbjct: 185 VGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGI 244

Query: 257 VVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSAQ 316
           VVAWKVKLVPVPA+VT+CSA R++EE A+K+  RWQYVANKL+E+LFL ILW GGK+++Q
Sbjct: 245 VVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQ 304

Query: 317 GGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIED 376
            GGK NP+A FF LFLG  +EL++ILN  FPEL   K++C E SWIES       F  E 
Sbjct: 305 EGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTE- 364

Query: 377 QPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGRM 436
             LEALL+RTP+ + SS K+KSDYVKEPM++  ++GIWKRL+SQDIEG  +  +PYGGRM
Sbjct: 365 LTLEALLSRTPLTNTSS-KIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM 424

Query: 437 SRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTAY 496
           S+ISES  PFSHRAG LY+IGYI GW+E+G++AE+RHL WIRE+Y YMTPFVSKSPR AY
Sbjct: 425 SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY 484

Query: 497 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIP 552
            NYRDLDIGSNNK+GK +YKQA V+G KYFGNNFNRLV VKTKVDPY+FF HEQSIP
Sbjct: 485 ANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 537

[Close]

BLAST of Cmc12g0327461.1 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 516.5 bits (1329), Expect = 2.6e-146
Identity = 252/505 (49.90%), Postives = 355/505 (70.30%), Query Frame = 0


Query: 48  FLQCLLHHSPDTSSINKLVHTPINSSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHI 107
           FLQCL     D++ ++K++HTP ++SFS++     +N RFS P+ PKP+LI+TP   S +
Sbjct: 37  FLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDV 96

Query: 108 QAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGA 167
           Q+AV C++  G+ IR RSGGHD+EGLSYV +  F+++DL NLRS+TVDV+  + WVQ+GA
Sbjct: 97  QSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGA 156

Query: 168 TLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDAN 227
           T+GELYY+IGKK+RTL FPAG+CPTVGVGGHFSGGGYG+LLRK+G+AAD+VIDA +VDA 
Sbjct: 157 TIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDAR 216

Query: 228 GEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVEEEAIKLID 287
           G   +R  MGED FWAIRGGGG SF VV++WK+ L+ VP++VT+ +  +  E+ A+K+I 
Sbjct: 217 GRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIH 276

Query: 288 RWQYVANKLEEDLFLAILWNGGKVSAQGGGKINPIALFFP-LFLGNVNELMAILNKTFPE 347
           RWQ+VA+K+ +DLF+ ++    K         N +   FP L+LG+V  L+ ++NK FPE
Sbjct: 277 RWQFVADKVSDDLFIRVMLQRYK---------NMVRASFPGLYLGSVKNLLKMVNKEFPE 336

Query: 348 LGFTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKT 407
           LG  +++C E SWIES    A   ++ ++P+  L  RT      +FK KSD+V+EPM KT
Sbjct: 337 LGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRT--RASLAFKAKSDFVQEPMPKT 396

Query: 408 AIEGIWKRLESQDIEGVTLALIPYGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVE 467
           AI  +W+RL+  + E   L   P+GG+MS I++   PF HR G +Y+I Y+  W  +G +
Sbjct: 397 AISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW--RG-D 456

Query: 468 AEERHLNWIREIYSYMTPFVSKSPRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGN 527
            +E+++ W+  +Y  M+ FV+KSPR AY+N RDLD+G      +  Y++   +G KYF N
Sbjct: 457 VKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKN 516

Query: 528 NFNRLVKVKTKVDPYNFFWHEQSIP 552
           NF RLV+VKT VDP +FF  EQSIP
Sbjct: 517 NFERLVRVKTSVDPSDFFCDEQSIP 524

[Close]

BLAST of Cmc12g0327461.1 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 508.4 bits (1308), Expect = 7.0e-144
Identity = 254/535 (47.48%), Postives = 366/535 (68.41%), Query Frame = 0


Query: 23  SLVFIVVCSSSSLWAATSS-SIKHEEFLQCLLHHSPDTSSINKLVHTPINSSFSTLFNFP 82
           S +F ++  S ++    S+    HE+FL+CL +   D +   K++HT  +SSF ++ +  
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 83  TRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHR- 142
            +N RFS   TPKP+ IITP   S +Q  + C++ HG+ +R RS GH +EGLSY+AY++ 
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 143 FIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFS 202
           F V+DL NLRS+++DV+  T WVQ+GAT GELYY+IGK +++L FPAGI PTVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 203 GGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKV 262
           GGGYG+LLRKYG+AADN+IDA +VDA+G   DR++MGED FWAIRGGGG SFGV+++WKV
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 263 KLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSAQGGGKIN 322
           KLV VP+++T+   +++ ++EA+++I +WQY A+K+ +DLF+       + + +   K  
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNA 304

Query: 323 PIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIEDQPLEAL 382
             ALF  L++G VN L+A++ + FPELG  KE C+E SWIES    A+    + + L  L
Sbjct: 305 VHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD--FPKGESLGVL 364

Query: 383 LNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGRMSRISES 442
            NR       SFK K D+V+EP+ + AI+ IW+RLE+ +     + L P+GG+MS ++E 
Sbjct: 365 TNRE--RTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEY 424

Query: 443 NIPFSHRAGILYQIGYILGW---DEKGVEAEERHLNWIREIYSYMTPFVSKSPRTAYVNY 502
             PF HR G LY+I Y+  W   ++K     +++L W+  +Y +MTP+VSKSPR AYVN+
Sbjct: 425 ETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNF 484

Query: 503 RDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPL 553
           +D+D+G      K  Y++   +G KYF NNF RLV+VKT+VDP +FF  EQSIPL
Sbjct: 485 KDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPL 528

[Close]

BLAST of Cmc12g0327461.1 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 506.5 bits (1303), Expect = 2.7e-143
Identity = 258/541 (47.69%), Postives = 369/541 (68.21%), Query Frame = 0


Query: 19  LVPLSLVFIVVCSSSSLWAATSSS-IKHEEFLQCLLHHSPDTSSINKLVHTPINSSFSTL 78
           L+ L + F+++  S S + + S+    HE FL+CL H   +  S  +++HT  + S+ ++
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSI 66

Query: 79  FNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVA 138
            N   +N RF    TPKP+ IITP   + +Q+ + C++ HG+ IR RSGGHD+EGLSY+A
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 139 YHR-FIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVG 198
             R F+V+DL NLRS+T+DV+  T WVQSGAT+GELYY+IGK S++L FPAG+ PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 199 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVV 258
           G F GGGYG+L+RKYG++ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF VV+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 259 AWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFL-AILWNGGKVSAQG 318
           +WK++L+ VP+ VT+    ++ E+EA+ +I++WQY+A+K+  DLF+ A+L    +V A  
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETEVYASF 306

Query: 319 GGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIEDQ 378
            G          L+LG V++L+A++   FPELG     C+E SWIES       + I+ +
Sbjct: 307 PG----------LYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL-----WFIKGE 366

Query: 379 PLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGRMS 438
            +E L  R   +   SFK K D+++EP+ KTAI+ +W+R E+ +     + L P+GG+MS
Sbjct: 367 SMEILAKRKRTSR--SFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMS 426

Query: 439 RISESNIPFSHRAGILYQIGYILGWDE---KGVEAEERHLNWIREIYSYMTPFVSKSPRT 498
            I+++ IPF HR G LY+I Y+  W E   K     E++L W+  +Y +MTP+VSKSPR 
Sbjct: 427 EIADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRR 486

Query: 499 AYVNYRDLDIGSNNKYG-KINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIP 553
           AYVN+RD+D+G       K  Y++A V+G KYF NNF+RLV+VKT VDP +FF  EQSIP
Sbjct: 487 AYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 528

[Close]

BLAST of Cmc12g0327461.1 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 498.8 bits (1283), Expect = 5.6e-141
Identity = 259/536 (48.32%), Postives = 358/536 (66.79%), Query Frame = 0


Query: 22  LSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSSFSTLFNFP 81
           L L F+V      +W ++SSS   E F QCL  +S     I+  +    N S+S++    
Sbjct: 7   LVLFFVVF-----IWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQAN 66

Query: 82  TRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAY--H 141
            RN RF+T +TPKP LII  ++ SH+QAA+ C K H LQ++IRSGGHD++GLSYV Y   
Sbjct: 67  IRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGK 126

Query: 142 RFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHF 201
            F V+D+ NLRSV VDV   TAWVQ+GA LGE+YY I +KS+TL +PAGICPTVGVGGH 
Sbjct: 127 PFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHI 186

Query: 202 SGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWK 261
           SGGGYG+++RKYG+  DN IDA +VD NG+  DR+ MGEDL+WAI GGGGGS+GVV+A+K
Sbjct: 187 SGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYK 246

Query: 262 VKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWN--GGKVSAQGGG 321
           + LV VP +VT+    R++E+ A  +I RWQ VA KL ++LF+  + +   G VS+Q   
Sbjct: 247 INLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTV 306

Query: 322 KINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIEDQPL 381
           +      F  +FLG+   L++ILN+ FPELG  + +C E SWI+S     N  Q+     
Sbjct: 307 R----TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN-IQVGSSET 366

Query: 382 EALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGRMSRI 441
             L    P+N+    K KSDYV+EP+++T +E IWK++   ++E  T+A  PYGG M RI
Sbjct: 367 LLLQRNQPVNY---LKRKSDYVREPISRTGLESIWKKM--IELEIPTMAFNPYGGEMGRI 426

Query: 442 SESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTAYVNY 501
           S +  PF +RAG L++I Y   W ++ +   +R++   R++Y +MTPFVSK+PR ++ NY
Sbjct: 427 SSTVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFNY 486

Query: 502 RDLDIGSNNKYGKI-NYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPL 553
           RD+D+G N+  GKI +Y +   +G KYF  NF RLVK+KT+VD  NFF +EQSIP+
Sbjct: 487 RDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPV 525

[Close]

BLAST of Cmc12g0327461.1 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 493.8 bits (1270), Expect = 1.8e-139
Identity = 251/543 (46.22%), Postives = 365/543 (67.22%), Query Frame = 0


Query: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPIN-- 73
           M +S  +PL  + ++  S  ++   +S +   ++F+ C+  ++     + K    P    
Sbjct: 1   MGISKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNV 60

Query: 74  SSFSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFE 133
           S FS +     +N RF   + PKP  I +P + SH+QA++ICSK   + +R+RSGGHD+E
Sbjct: 61  SMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYE 120

Query: 134 GLSYVAY--HRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGI 193
           GLSYV+     FI++DL  +R V ++++ ++AWVQSGAT+GELYY+I +KS+  GFPAG+
Sbjct: 121 GLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGL 180

Query: 194 CPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGG 253
           C ++G+GGH +GG YGS++RKYG+ ADNV+DA +VDANG+  DR +MGED FWAIRGG G
Sbjct: 181 CSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAG 240

Query: 254 GSFGVVVAWKVKLVPVPASVTICSAKRSVEEE-AIKLIDRWQYVANKLEEDLFLAILWNG 313
           GSFG+++AWK+KLVPVP +VT+ +  ++++++   K+I +WQ VA+KL E+LF+ +L+N 
Sbjct: 241 GSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFN- 300

Query: 314 GKVSAQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAAN 373
             V+  GG K      +  LFLG    LM ++ K+FPELG T ++C E SW+ES A  + 
Sbjct: 301 --VAGTGGNK-TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISG 360

Query: 374 RFQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALI 433
            F         L  ++P     SFK KSD+VK P+ ++ ++GI+K+L  +DI    +   
Sbjct: 361 -FPTHTPTNVLLQGKSPFPK-VSFKAKSDFVKTPIPESGLQGIFKKLLKEDIP--LMIWN 420

Query: 434 PYGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSK 493
           PYGG M++I ES IPF HR G+L+++ Y+  W +   +   RH+NWIR++YSYMTP+VS 
Sbjct: 421 PYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSS 480

Query: 494 SPRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQ 552
           +PR AYVNYRDLD+G N K  K   KQA V+G+ YF NNFNRL+ +K KVDP NFF HEQ
Sbjct: 481 NPREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQ 534

The following BLAST results are available for this feature:

BLAST of Cmc12g0327461.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008445721.1	0.0e+00	100.00	PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo]	[more]
KAA0047096.1	6.1e-299	95.76	tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05062.1...	[more]
XP_011659211.2	4.1e-295	92.25	tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653319.1 hypothetica...	[more]
XP_038895262.1	2.3e-258	81.77	tetrahydrocannabinolic acid synthase-like [Benincasa hispida]	[more]
XP_038895343.1	1.4e-250	79.78	cannabidiolic acid synthase-like [Benincasa hispida]	[more]

BLAST of Cmc12g0327461.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GTB6	2.7e-148	50.78	Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV...	[more]
A6P6V9	1.5e-146	49.17	Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1	[more]
A6P6W1	1.9e-146	50.39	Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=...	[more]
A6P6W0	5.6e-146	50.20	Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=...	[more]
Q9SVG5	3.6e-145	49.90	Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=...	[more]

BLAST of Cmc12g0327461.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BDD9	0.0e+00	100.00	tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348866...	[more]
A0A5D3C1K3	3.0e-299	95.76	Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A5A7TYU2	1.4e-245	77.37	Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BCV7	1.4e-245	77.37	cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV...	[more]
A0A6J1CN68	1.4e-224	71.69	tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]

BLAST of Cmc12g0327461.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20820.1	2.6e-146	49.90	FAD-binding Berberine family protein	[more]
AT5G44440.1	7.0e-144	47.48	FAD-binding Berberine family protein	[more]
AT5G44410.1	2.7e-143	47.69	FAD-binding Berberine family protein	[more]
AT1G30700.1	5.6e-141	48.32	FAD-binding Berberine family protein	[more]
AT5G44400.1	1.8e-139	46.22	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012951	Berberine/berberine-like	PFAM	PF08031	BBE	coord: 493..551 e-value: 8.5E-21 score: 73.8
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 95..231 e-value: 2.4E-28 score: 98.6
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 153..551 e-value: 9.6E-158 score: 527.5
IPR016167	FAD-binding, type PCMH, subdomain 1	GENE3D	3.30.43.10		coord: 42..149 e-value: 1.0E-40 score: 139.9
None	No IPR available	GENE3D	3.40.462.20		coord: 268..489 e-value: 9.6E-158 score: 527.5
None	No IPR available	PANTHER	PTHR32448:SF131	RETICULINE OXIDASE-LIKE PROTEIN	coord: 22..552
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 22..552
IPR016166	FAD-binding domain, PCMH-type	PROSITE	PS51387	FAD_PCMH	coord: 91..265 score: 20.193354
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 42..266

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cmc12g0327461	Cmc12g0327461	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc12g0327461.1-exon	Cmc12g0327461.1-exon-CMiso1.1chr12:18402861..18403987	exon
Cmc12g0327461.1-exon	Cmc12g0327461.1-exon-CMiso1.1chr12:18404502..18405518	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc12g0327461.1-three_prime_utr	Cmc12g0327461.1-three_prime_utr-CMiso1.1chr12:18402861..18403238	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc12g0327461.1-cds	Cmc12g0327461.1-cds-CMiso1.1chr12:18403239..18403987	CDS
Cmc12g0327461.1-cds	Cmc12g0327461.1-cds-CMiso1.1chr12:18404502..18405423	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc12g0327461.1-five_prime_utr	Cmc12g0327461.1-five_prime_utr-CMiso1.1chr12:18405424..18405518	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cmc12g0327461.1	Cmc12g0327461.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016491	oxidoreductase activity