Cmc05g0140291.1 (mRNA) Melon (Charmono) v1.1

Overview


Name	Cmc05g0140291.1
Type	mRNA
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Reverse transcriptase
Location	CMiso1.1chr05: 24737685 .. 24738808 (-)
Sequence length	759
RNA-Seq Expression	Cmc05g0140291.1
Synteny	Cmc05g0140291.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCCAGCAGAGTTGAAAGAGCTGAAAGTGCAGTTACAGGAGTTGCTTGATAAAGGCTTCATTCGGCCGAGTGTGTCACCTTGGGGTGCACCAGTTTTATTTGTTAAGAAGAAGGATGGATCGATGCGCCTATGTATTGACTATAGAGAGTTGAATAAGGTAACCGTTAAGAACAAATATCCCTTGCCCAGGATCGATGATCTGTTTGACCAATTACAGGGAGCTACAGTGTTCTTTAAGATCGACCTTCGGTCAGGATATCATCAGTTGAAGATTAAGGATAGCGATGTACCGAAGACAGTTTTTGTTCTAGATATGGACACTATGAGTTTATTGTGATGTCTTTTTGTTTGACAAATGCTCCGACAGTGTTCAGGGATTTGATGAACAGAGTGTTTATAAAGTTCCTAGACACTTTTGTGATCGTGTTTATTGATGATATTTTGATATATTCCAAGACAGAGGCCGAGCATGAGGAGCATTTACGTATGGTTCTAGAAACCCTTCGAGCTAATAAATTGTATGCAAAGTTCTCAAAATGTGAGTTTTGGTTTAAGCAGGTATCCTTTCTAGGCCATGTGGTTTCTAAAGCTGGTGTTTCTGTGGATCCAACTAAGATAGAGGCAGTCACGAGTTGACCCCGACCTTCCACAGTCAGTGAGGTTCATAGCTTTCTGGGTTTAGCAGGTTATTTCTGACGATTTATGGAGAACTTTTCCCGTATAGCTACTCCTCTTACTCAGTTGACCAGGAAGGGAGCTCCTTTTGTTTGGAGCAAGGCTTGTGAGGACAGTTTCCAGAACCTTAGACAGAAGCTACCTGCACTGGTTCTTACTGTACCTGATGGTTCAGGGAGTTTTGTGATTTACAGTGATGCTTCTAAGAAAGGTTTGGGTTGTGTTTTGATGCAGCAAGGTAAGGTAGTCGCTTACACTTCTCGTCAGTTGAAGAGTCATGAGCAGAATTACCCTACCCACGATTTAGAAATGGCAGTAGTAATTTTTGCCTTGAAGATATGGAGGCATTACTTGTATGGTAAAAAGATACAGATCTTCACAGATCATAAAGGCTTGAAATACATCTTCACTGAAAAGGAACTGAATATGAGACAACGAAGATGA

mRNA sequence

ATGGCCCCAGCAGAGTTGAAAGAGCTGAAAGTGCAGTTACAGGAGTTGCTTGATAAAGGCTTCATTCGGCCGAGTGTGTCACCTTGGGGTGCACCAGTTTTATTTGTTAAGAAGAAGGATGGATCGATGCGCCTATGTATTGACTATAGAGAGTTGAATAAGGTAACCGTTAAGAACAAATATCCCTTGCCCAGGATCGATGATCTGTTTGACCAATTACAGGGAGCTACAGTGTTCTTTAAGATCGACCTTCGGTCAGGATATCATCAGTTGAAGATTAAGGATAGCGATGTATCCTTTCTAGGCCATGTGGTTTCTAAAGCTGGTGTTTCTGTGGATCCAACTAAGATAGAGGCAGTCACGACTACTCCTCTTACTCAGTTGACCAGGAAGGGAGCTCCTTTTGTTTGGAGCAAGGCTTGTGAGGACAGTTTCCAGAACCTTAGACAGAAGCTACCTGCACTGGTTCTTACTGTACCTGATGGTTCAGGGAGTTTTGTGATTTACAGTGATGCTTCTAAGAAAGGTTTGGGTTGTGTTTTGATGCAGCAAGGTAAGGTAGTCGCTTACACTTCTCGTCAGTTGAAGAGTCATGAGCAGAATTACCCTACCCACGATTTAGAAATGGCAGTAGTAATTTTTGCCTTGAAGATATGGAGGCATTACTTGTATGGTAAAAAGATACAGATCTTCACAGATCATAAAGGCTTGAAATACATCTTCACTGAAAAGGAACTGAATATGAGACAACGAAGATGA

Coding sequence (CDS)

ATGGCCCCAGCAGAGTTGAAAGAGCTGAAAGTGCAGTTACAGGAGTTGCTTGATAAAGGCTTCATTCGGCCGAGTGTGTCACCTTGGGGTGCACCAGTTTTATTTGTTAAGAAGAAGGATGGATCGATGCGCCTATGTATTGACTATAGAGAGTTGAATAAGGTAACCGTTAAGAACAAATATCCCTTGCCCAGGATCGATGATCTGTTTGACCAATTACAGGGAGCTACAGTGTTCTTTAAGATCGACCTTCGGTCAGGATATCATCAGTTGAAGATTAAGGATAGCGATGTATCCTTTCTAGGCCATGTGGTTTCTAAAGCTGGTGTTTCTGTGGATCCAACTAAGATAGAGGCAGTCACGACTACTCCTCTTACTCAGTTGACCAGGAAGGGAGCTCCTTTTGTTTGGAGCAAGGCTTGTGAGGACAGTTTCCAGAACCTTAGACAGAAGCTACCTGCACTGGTTCTTACTGTACCTGATGGTTCAGGGAGTTTTGTGATTTACAGTGATGCTTCTAAGAAAGGTTTGGGTTGTGTTTTGATGCAGCAAGGTAAGGTAGTCGCTTACACTTCTCGTCAGTTGAAGAGTCATGAGCAGAATTACCCTACCCACGATTTAGAAATGGCAGTAGTAATTTTTGCCTTGAAGATATGGAGGCATTACTTGTATGGTAAAAAGATACAGATCTTCACAGATCATAAAGGCTTGAAATACATCTTCACTGAAAAGGAACTGAATATGAGACAACGAAGATGA

Protein sequence

MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNKYPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSDVSFLGHVVSKAGVSVDPTKIEAVTTTPLTQLTRKGAPFVWSKACEDSFQNLRQKLPALVLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYTSRQLKSHEQNYPTHDLEMAVVIFALKIWRHYLYGKKIQIFTDHKGLKYIFTEKELNMRQRR

Homology

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BLAST of Cmc05g0140291.1 vs. NCBI nr
Match: KAA0031895.1 (ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 438.3 bits (1126), Expect = 4.4e-119
Identity = 234/328 (71.34%), Postives = 241/328 (73.48%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 60
           MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKN+
Sbjct: 479 MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 538

Query: 61  YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSD----------------------- 120
           YPLPRIDDLFDQLQGATVF KIDLRSGYHQL+IKD D                       
Sbjct: 539 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDEDVPKTAFRSRYGHYEFIVMSFGLT 598

Query: 121 ---------------------VSFLGHVVSKAGVSVDPTKIEAVT--------------- 180
                                VSFLGHVVSKAGVSVDP KIEAVT               
Sbjct: 599 NAPAVFMDLMNRVFREFLDTFVSFLGHVVSKAGVSVDPAKIEAVTGWTRPSTVSEVRSFL 658

Query: 181 ----------------TTPLTQLTRKGAPFVWSKACEDSFQNLRQKL-PALVLTVPDGSG 240
                            TPLTQLTRKGAPFVWSKACEDSFQNL+QKL  A VLTVPDGSG
Sbjct: 659 GLAGYYRRFVGNFSRIATPLTQLTRKGAPFVWSKACEDSFQNLKQKLVTAPVLTVPDGSG 718

Query: 241 SFVIYSDASKKGLGCVLMQQGKVVAYTSRQLKSHEQNYPTHDLEMAVVIFALKIWRHYLY 253
           SFVIYSDASKKGLGCVLMQQGKVVAY SRQLKSHEQNY THDLE+A V+FALKIWRHYLY
Sbjct: 719 SFVIYSDASKKGLGCVLMQQGKVVAYASRQLKSHEQNYSTHDLELAAVVFALKIWRHYLY 778

[Close]

BLAST of Cmc05g0140291.1 vs. NCBI nr
Match: KAA0067622.1 (ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 436.8 bits (1122), Expect = 1.3e-118
Identity = 233/344 (67.73%), Postives = 241/344 (70.06%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 60
           MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKN+
Sbjct: 396 MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 455

Query: 61  YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSD----------------------- 120
           YPLPRIDDLFDQLQGATVF KIDLRSGYHQL+IKD D                       
Sbjct: 456 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDEDVPKTAFRSRYGHYEFIVMSFGLT 515

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 516 NAPAVFMDLMNRVFREFLDTFVIVFIDDILIYSKTDAEHEEHLRMVLQTLRDNKLYAKFS 575

Query: 181 --------VSFLGHVVSKAGVSVDPTKIEAVTTTPLTQLTRKGAPFVWSKACEDSFQNLR 240
                   V+FLGHVVSKAGVSVDP KIEAVT TPLTQLTRKG PFVWSKACEDSFQNL+
Sbjct: 576 KCEFWLKQVTFLGHVVSKAGVSVDPAKIEAVTATPLTQLTRKGVPFVWSKACEDSFQNLK 635

Query: 241 QKL-PALVLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYTSRQLKSHEQNYPTHDLE 253
           QKL  A VLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAY SRQLKSHEQNYPTHDLE
Sbjct: 636 QKLVTAPVLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYASRQLKSHEQNYPTHDLE 695

[Close]

BLAST of Cmc05g0140291.1 vs. NCBI nr
Match: KAA0057633.1 (ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 436.4 bits (1121), Expect = 1.7e-118
Identity = 228/315 (72.38%), Postives = 238/315 (75.56%), Query Frame = 0


Query: 1    MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 60
            MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKN+
Sbjct: 893  MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 952

Query: 61   YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKD------------------------- 120
            Y LPRIDD FDQLQGATVF KIDLRSGYH L+IKD                         
Sbjct: 953  YSLPRIDDQFDQLQGATVFSKIDLRSGYHHLRIKDVFMDLMNRVFREFLDTFVIVFIDDI 1012

Query: 121  -------------------------------------SDVSFLGHVVSKAGVSVDPTKIE 180
                                                   VSFLGHVVSKAGVSVDP KIE
Sbjct: 1013 LIYSKTEAKYEEHLRIVLQTLRDTKLYAKFSKCEFWLKQVSFLGHVVSKAGVSVDPAKIE 1072

Query: 181  AVTTTPLTQLTRKGAPFVWSKACEDSFQNLRQKL-PALVLTVPDGSGSFVIYSDASKKGL 240
            AVT TPLTQLTRKGAPFVWSKACEDSFQNL+QKL  A VLTVPDGSGSF+IYSDASKKGL
Sbjct: 1073 AVTATPLTQLTRKGAPFVWSKACEDSFQNLKQKLVTAPVLTVPDGSGSFLIYSDASKKGL 1132

Query: 241  GCVLMQQGKVVAYTSRQLKSHEQNYPTHDLEMAVVIFALKIWRHYLYGKKIQIFTDHKGL 253
            GCVLMQQGKVVAY SRQLKS++QNYPTHDLE+A V+FALKIWRHYLYG+KIQIFTDHK L
Sbjct: 1133 GCVLMQQGKVVAYASRQLKSYDQNYPTHDLELAAVVFALKIWRHYLYGEKIQIFTDHKSL 1192

[Close]

BLAST of Cmc05g0140291.1 vs. NCBI nr
Match: KAA0025469.1 (ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 431.4 bits (1108), Expect = 5.3e-117
Identity = 232/344 (67.44%), Postives = 239/344 (69.48%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 60
           MAPAELKELK+QLQE LDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK
Sbjct: 599 MAPAELKELKMQLQEFLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 658

Query: 61  YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSD----------------------- 120
           YPLPRIDDLFDQLQGATVF KIDLRSGYHQL+IKD D                       
Sbjct: 659 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDGDVPKTAFRSRYGHYEFIVMSFGLT 718

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 719 NAPTVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRIVLQTLRDNKLYAKFS 778

Query: 181 --------VSFLGHVVSKAGVSVDPTKIEAVTTTPLTQLTRKGAPFVWSKACEDSFQNLR 240
                   VSFLGHVVSKAGVSVDP KIEAVT TPLTQLTRKGAPFVWSKACEDSFQNL+
Sbjct: 779 KCEFWLKQVSFLGHVVSKAGVSVDPAKIEAVTATPLTQLTRKGAPFVWSKACEDSFQNLK 838

Query: 241 QKL-PALVLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYTSRQLKSHEQNYPTHDLE 253
           QKL  A VLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAY S QLKSHEQN PTHDLE
Sbjct: 839 QKLVTAPVLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYASCQLKSHEQNCPTHDLE 898

[Close]

BLAST of Cmc05g0140291.1 vs. NCBI nr
Match: KAA0057623.1 (ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa] >TYK14866.1 ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 427.9 bits (1099), Expect = 5.9e-116
Identity = 229/337 (67.95%), Postives = 238/337 (70.62%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 60
           MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKK DGSMRLCIDYRELNKVTVKN+
Sbjct: 619 MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKNDGSMRLCIDYRELNKVTVKNR 678

Query: 61  YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSD----------------------- 120
           YPLPRIDDLFDQLQGATVFFKIDLRSGYHQL+IKD D                       
Sbjct: 679 YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLRIKDGDVPKMAFPSRYGHYEFIVMSFEAE 738

Query: 121 ------------------------------VSFLGHVVSKAGVSVDPTKIEAV------- 180
                                         VSFLGHVVSKAGV VDP KIE V       
Sbjct: 739 HEEHLRMVLQTLRDNKLYAKFSKCEFWLKHVSFLGHVVSKAGVYVDPAKIEVVINWPRPS 798

Query: 181 ------------------------TTTPLTQLTRKGAPFVWSKACEDSFQNLRQKL-PAL 240
                                     TPLTQLTRKGAPFVWSKACEDSFQ+L+QKL  A 
Sbjct: 799 TDSEVHSFLGLAGYYRRFVENFSRIATPLTQLTRKGAPFVWSKACEDSFQSLKQKLVTAR 858

Query: 241 VLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYTSRQLKSHEQNYPTHDLEMAVVIFA 253
           VLT+PDGS SFVIYSDASKKGLGCVLMQQGKVVAY SRQLKSHEQNYPTHDLE+A V+FA
Sbjct: 859 VLTIPDGSRSFVIYSDASKKGLGCVLMQQGKVVAYASRQLKSHEQNYPTHDLELAAVVFA 918

[Close]

BLAST of Cmc05g0140291.1 vs. ExPASy Swiss-Prot
Match: P10394 (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.9e-29
Identity = 97/373 (26.01%), Postives = 150/373 (40.21%), Query Frame = 0


Query: 4   AELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDG------SMRLCIDYRELNKVTV 63
           ++++E++ Q+Q+L+    + PSVS + +P+L V KK          RL IDYR++NK  +
Sbjct: 325 SQVEEIQAQVQKLIKDKIVEPSVSQYNSPLLLVPKKSSPNSDKKKWRLVIDYRQINKKLL 384

Query: 64  KNKYPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDS--------------------- 123
            +K+PLPRIDD+ DQL  A  F  +DL SG+HQ+++ +                      
Sbjct: 385 ADKFPLPRIDDILDQLGRAKYFSCLDLMSGFHQIELDEGSRDITSFSTSNGSYRFTRLPF 444

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 445 GLKIAPNSFQRMMTIAFSGIEPSQAFLYMDDLIVIGCSEKHMLKNLTEVFGKCREYNLKL 504

Query: 184 ----------DVSFLGHVVSKAGVSVDPTKIEAVTTTP---------------------- 243
                     +V+FLGH  +  G+  D  K + +   P                      
Sbjct: 505 HPEKCSFFMHEVTFLGHKCTDKGILPDDKKYDVIQNYPVPHDADSARRFVAFCNYYRRFI 564

[Close]

BLAST of Cmc05g0140291.1 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.7e-28
Identity = 97/375 (25.87%), Postives = 154/375 (41.07%), Query Frame = 0


Query: 7   KELKVQLQELLDKGFIRPSVSPWGAPVLFV-KKKDGS----MRLCIDYRELNKVTVKNKY 66
           +E++ Q+Q++L++G IR S SP+ +P+  V KK+D S     R+ IDYR+LN++TV +++
Sbjct: 221 QEVESQIQDMLNQGIIRTSNSPYNSPIWVVPKKQDASGKQKFRIVIDYRKLNEITVGDRH 280

Query: 67  PLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSDVS---------------------- 126
           P+P +D++  +L     F  IDL  G+HQ+++    VS                      
Sbjct: 281 PIPNMDEILGKLGRCNYFTTIDLAKGFHQIEMDPESVSKTAFSTKHGHYEYLRMPFGLKN 340

Query: 127 ------------------------------------------------------------ 186
                                                                       
Sbjct: 341 APATFQRCMNDILRPLLNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKANLKLQLDK 400

Query: 187 ---------FLGHVVSKAGVSVDPTKIEAVTTTP-------------LTQLTRKGAPFVW 246
                    FLGHV++  G+  +P KIEA+   P             LT   RK  P   
Sbjct: 401 CEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEIKAFLGLTGYYRKFIPNFA 460

Query: 247 SKA---------------CEDSFQNLRQKLPAL-----VLTVPDGSGSFVIYSDASKKGL 253
             A                   + +  +KL  L     +L VPD +  F + +DAS   L
Sbjct: 461 DIAKPMTKCLKKNMKIDTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVAL 520

[Close]

BLAST of Cmc05g0140291.1 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.4e-27
Identity = 94/374 (25.13%), Postives = 151/374 (40.37%), Query Frame = 0


Query: 8   ELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKD-----GSMRLCIDYRELNKVTVKNKYP 67
           E++ Q+QE+L++G IR S SP+ +P   V KK         R+ IDYR+LN++T+ ++YP
Sbjct: 221 EVENQVQEMLNQGLIRESNSPYNSPTWVVPKKPDASGANKYRVVIDYRKLNEITIPDRYP 280

Query: 68  LPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSDVS----------------------- 127
           +P +D++  +L     F  IDL  G+HQ+++ +  +S                       
Sbjct: 281 IPNMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAFSTKSGHYEYLRMPFGLRNA 340

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 341 PATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSIQLVFTKLADANLKLQLDKC 400

Query: 188 --------FLGHVVSKAGVSVDPTKIEAVTTTP-------------LTQLTRKGAP---- 247
                   FLGH+V+  G+  +P K++A+ + P             LT   RK  P    
Sbjct: 401 EFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYPIPTKDKEIRAFLGLTGYYRKFIPNYAD 460

Query: 248 ------FVWSKACEDSFQNLR-----QKLPAL-----VLTVPDGSGSFVIYSDASKKGLG 253
                     K  +   Q L      +KL AL     +L +PD    FV+ +DAS   LG
Sbjct: 461 IAKPMTSCLKKRTKIDTQKLEYIEAFEKLKALIIRDPILQLPDFEKKFVLTTDASNLALG 520

[Close]

BLAST of Cmc05g0140291.1 vs. ExPASy Swiss-Prot
Match: P10401 (Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.9e-26
Identity = 93/383 (24.28%), Postives = 153/383 (39.95%), Query Frame = 0


Query: 12  QLQELLDKGFIRPSVSPWGAPVLFVKKK------DGSMRLCIDYRELNKVTVKNKYPLPR 71
           ++++LL  G IRPS SP+ +P   V KK      + + RL ID+R+LN+ T+ ++YP+P 
Sbjct: 200 EVKQLLKDGIIRPSRSPYNSPTWVVDKKGTDAFGNPNKRLVIDFRKLNEKTIPDRYPMPS 259

Query: 72  IDDLFDQLQGATVFFKIDLRSGYHQLKIKDSD---------------------------- 131
           I  +   L  A  F  +DL+SGYHQ+ + + D                            
Sbjct: 260 IPMILANLGKAKFFTTLDLKSGYHQIYLAEHDREKTSFSVNGGKYEFCRLPFGLRNASSI 319

Query: 132 ------------------------------------------------------------ 191
                                                                       
Sbjct: 320 FQRALDDVLREQIGKICYVYVDDVIIFSENESDHVRHIDTVLKCLIDANMRVSQEKTRFF 379

Query: 192 ---VSFLGHVVSKAGVSVDPTKIEAV-------------------------------TTT 251
              V +LG +VSK G   DP K++A+                                  
Sbjct: 380 KESVEYLGFIVSKDGTKSDPEKVKAIQEYPEPDCVYKVRSFLGLASYYRVFIKDFAAIAR 439

Query: 252 PLTQLTR-----------KGAPFVWSKACEDSFQNLRQKLPA--LVLTVPDGSGSFVIYS 253
           P+T + +           K  P  +++   ++FQ LR  L +  ++L  PD    F + +
Sbjct: 440 PITDILKGENGSVSKHMSKKIPVEFNETQRNAFQRLRNILASEDVILKYPDFKKPFDLTT 499

[Close]

BLAST of Cmc05g0140291.1 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.6e-23
Identity = 94/389 (24.16%), Postives = 146/389 (37.53%), Query Frame = 0


Query: 8   ELKVQLQELLDKGFIRPSVSPWGAPVLFVKKK-----DGSMRLCIDYRELNKVTVKNKYP 67
           E++ Q+ ELL  G IRPS SP+ +P+  V KK     +   R+ +D++ LN VT+ + YP
Sbjct: 138 EVERQIDELLQDGIIRPSNSPYNSPIWIVPKKPKPNGEKQYRMVVDFKRLNTVTIPDTYP 197

Query: 68  LPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSD------------------------- 127
           +P I+     L  A  F  +DL SG+HQ+ +K+SD                         
Sbjct: 198 IPDINATLASLGNAKYFTTLDLTSGFHQIHMKESDIPKTAFSTLNGKYEFLRLPFGLKNA 257

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 258 PAIFQRMIDDILREHIGKVCYVYIDDIIVFSEDYDTHWKNLRLVLASLSKANLQVNLEKS 317

Query: 188 ------VSFLGHVVSKAGVSVDPTKIEAVT------------------------------ 247
                 V FLG++V+  G+  DP K+ A++                              
Sbjct: 318 HFLDTQVEFLGYIVTADGIKADPKKVRAISEMPPPTSVKELKRFLGMTSYYRKFIQDYAK 377

Query: 248 -TTPLTQLTR-----------KGAPFVWSKACEDSFQNLRQKL-PALVLTVPDGSGSFVI 253
              PLT LTR              P    +    SF +L+  L  + +L  P  +  F +
Sbjct: 378 VAKPLTNLTRGLYANIKSSQSSKVPITLDETALQSFNDLKSILCSSEILAFPCFTKPFHL 437

[Close]

BLAST of Cmc05g0140291.1 vs. ExPASy TrEMBL
Match: A0A5A7SRV9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold218G00130 PE=4 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 2.1e-119
Identity = 234/328 (71.34%), Postives = 241/328 (73.48%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 60
           MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKN+
Sbjct: 479 MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 538

Query: 61  YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSD----------------------- 120
           YPLPRIDDLFDQLQGATVF KIDLRSGYHQL+IKD D                       
Sbjct: 539 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDEDVPKTAFRSRYGHYEFIVMSFGLT 598

Query: 121 ---------------------VSFLGHVVSKAGVSVDPTKIEAVT--------------- 180
                                VSFLGHVVSKAGVSVDP KIEAVT               
Sbjct: 599 NAPAVFMDLMNRVFREFLDTFVSFLGHVVSKAGVSVDPAKIEAVTGWTRPSTVSEVRSFL 658

Query: 181 ----------------TTPLTQLTRKGAPFVWSKACEDSFQNLRQKL-PALVLTVPDGSG 240
                            TPLTQLTRKGAPFVWSKACEDSFQNL+QKL  A VLTVPDGSG
Sbjct: 659 GLAGYYRRFVGNFSRIATPLTQLTRKGAPFVWSKACEDSFQNLKQKLVTAPVLTVPDGSG 718

Query: 241 SFVIYSDASKKGLGCVLMQQGKVVAYTSRQLKSHEQNYPTHDLEMAVVIFALKIWRHYLY 253
           SFVIYSDASKKGLGCVLMQQGKVVAY SRQLKSHEQNY THDLE+A V+FALKIWRHYLY
Sbjct: 719 SFVIYSDASKKGLGCVLMQQGKVVAYASRQLKSHEQNYSTHDLELAAVVFALKIWRHYLY 778

[Close]

BLAST of Cmc05g0140291.1 vs. ExPASy TrEMBL
Match: A0A5A7VGW8 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold485G00940 PE=4 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 6.1e-119
Identity = 233/344 (67.73%), Postives = 241/344 (70.06%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 60
           MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKN+
Sbjct: 396 MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 455

Query: 61  YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSD----------------------- 120
           YPLPRIDDLFDQLQGATVF KIDLRSGYHQL+IKD D                       
Sbjct: 456 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDEDVPKTAFRSRYGHYEFIVMSFGLT 515

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 516 NAPAVFMDLMNRVFREFLDTFVIVFIDDILIYSKTDAEHEEHLRMVLQTLRDNKLYAKFS 575

Query: 181 --------VSFLGHVVSKAGVSVDPTKIEAVTTTPLTQLTRKGAPFVWSKACEDSFQNLR 240
                   V+FLGHVVSKAGVSVDP KIEAVT TPLTQLTRKG PFVWSKACEDSFQNL+
Sbjct: 576 KCEFWLKQVTFLGHVVSKAGVSVDPAKIEAVTATPLTQLTRKGVPFVWSKACEDSFQNLK 635

Query: 241 QKL-PALVLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYTSRQLKSHEQNYPTHDLE 253
           QKL  A VLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAY SRQLKSHEQNYPTHDLE
Sbjct: 636 QKLVTAPVLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYASRQLKSHEQNYPTHDLE 695

[Close]

BLAST of Cmc05g0140291.1 vs. ExPASy TrEMBL
Match: A0A5A7UP36 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold9731G00040 PE=4 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 8.0e-119
Identity = 228/315 (72.38%), Postives = 238/315 (75.56%), Query Frame = 0


Query: 1    MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 60
            MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKN+
Sbjct: 893  MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 952

Query: 61   YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKD------------------------- 120
            Y LPRIDD FDQLQGATVF KIDLRSGYH L+IKD                         
Sbjct: 953  YSLPRIDDQFDQLQGATVFSKIDLRSGYHHLRIKDVFMDLMNRVFREFLDTFVIVFIDDI 1012

Query: 121  -------------------------------------SDVSFLGHVVSKAGVSVDPTKIE 180
                                                   VSFLGHVVSKAGVSVDP KIE
Sbjct: 1013 LIYSKTEAKYEEHLRIVLQTLRDTKLYAKFSKCEFWLKQVSFLGHVVSKAGVSVDPAKIE 1072

Query: 181  AVTTTPLTQLTRKGAPFVWSKACEDSFQNLRQKL-PALVLTVPDGSGSFVIYSDASKKGL 240
            AVT TPLTQLTRKGAPFVWSKACEDSFQNL+QKL  A VLTVPDGSGSF+IYSDASKKGL
Sbjct: 1073 AVTATPLTQLTRKGAPFVWSKACEDSFQNLKQKLVTAPVLTVPDGSGSFLIYSDASKKGL 1132

Query: 241  GCVLMQQGKVVAYTSRQLKSHEQNYPTHDLEMAVVIFALKIWRHYLYGKKIQIFTDHKGL 253
            GCVLMQQGKVVAY SRQLKS++QNYPTHDLE+A V+FALKIWRHYLYG+KIQIFTDHK L
Sbjct: 1133 GCVLMQQGKVVAYASRQLKSYDQNYPTHDLELAAVVFALKIWRHYLYGEKIQIFTDHKSL 1192

[Close]

BLAST of Cmc05g0140291.1 vs. ExPASy TrEMBL
Match: A0A5A7SJH3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold417G00700 PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 2.6e-117
Identity = 232/344 (67.44%), Postives = 239/344 (69.48%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 60
           MAPAELKELK+QLQE LDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK
Sbjct: 599 MAPAELKELKMQLQEFLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 658

Query: 61  YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSD----------------------- 120
           YPLPRIDDLFDQLQGATVF KIDLRSGYHQL+IKD D                       
Sbjct: 659 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDGDVPKTAFRSRYGHYEFIVMSFGLT 718

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 719 NAPTVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRIVLQTLRDNKLYAKFS 778

Query: 181 --------VSFLGHVVSKAGVSVDPTKIEAVTTTPLTQLTRKGAPFVWSKACEDSFQNLR 240
                   VSFLGHVVSKAGVSVDP KIEAVT TPLTQLTRKGAPFVWSKACEDSFQNL+
Sbjct: 779 KCEFWLKQVSFLGHVVSKAGVSVDPAKIEAVTATPLTQLTRKGAPFVWSKACEDSFQNLK 838

Query: 241 QKL-PALVLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYTSRQLKSHEQNYPTHDLE 253
           QKL  A VLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAY S QLKSHEQN PTHDLE
Sbjct: 839 QKLVTAPVLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYASCQLKSHEQNCPTHDLE 898

[Close]

BLAST of Cmc05g0140291.1 vs. ExPASy TrEMBL
Match: A0A5A7UP12 (Ty3-gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1432G00010 PE=4 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 2.8e-116
Identity = 229/337 (67.95%), Postives = 238/337 (70.62%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNK 60
           MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKK DGSMRLCIDYRELNKVTVKN+
Sbjct: 619 MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKNDGSMRLCIDYRELNKVTVKNR 678

Query: 61  YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLKIKDSD----------------------- 120
           YPLPRIDDLFDQLQGATVFFKIDLRSGYHQL+IKD D                       
Sbjct: 679 YPLPRIDDLFDQLQGATVFFKIDLRSGYHQLRIKDGDVPKMAFPSRYGHYEFIVMSFEAE 738

Query: 121 ------------------------------VSFLGHVVSKAGVSVDPTKIEAV------- 180
                                         VSFLGHVVSKAGV VDP KIE V       
Sbjct: 739 HEEHLRMVLQTLRDNKLYAKFSKCEFWLKHVSFLGHVVSKAGVYVDPAKIEVVINWPRPS 798

Query: 181 ------------------------TTTPLTQLTRKGAPFVWSKACEDSFQNLRQKL-PAL 240
                                     TPLTQLTRKGAPFVWSKACEDSFQ+L+QKL  A 
Sbjct: 799 TDSEVHSFLGLAGYYRRFVENFSRIATPLTQLTRKGAPFVWSKACEDSFQSLKQKLVTAR 858

Query: 241 VLTVPDGSGSFVIYSDASKKGLGCVLMQQGKVVAYTSRQLKSHEQNYPTHDLEMAVVIFA 253
           VLT+PDGS SFVIYSDASKKGLGCVLMQQGKVVAY SRQLKSHEQNYPTHDLE+A V+FA
Sbjct: 859 VLTIPDGSRSFVIYSDASKKGLGCVLMQQGKVVAYASRQLKSHEQNYPTHDLELAAVVFA 918

The following BLAST results are available for this feature:

BLAST of Cmc05g0140291.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0031895.1	4.4e-119	71.34	ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0067622.1	1.3e-118	67.73	ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0057633.1	1.7e-118	72.38	ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0025469.1	5.3e-117	67.44	ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0057623.1	5.9e-116	67.95	ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa] >TYK14866.1 ty3-gyp...	[more]

BLAST of Cmc05g0140291.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10394	1.9e-29	26.01	Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaste...	[more]
P04323	3.7e-28	25.87	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
P20825	1.4e-27	25.13	Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste...	[more]
P10401	5.9e-26	24.28	Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogas...	[more]
Q8I7P9	1.6e-23	24.16	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]

BLAST of Cmc05g0140291.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SRV9	2.1e-119	71.34	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21...	[more]
A0A5A7VGW8	6.1e-119	67.73	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold48...	[more]
A0A5A7UP36	8.0e-119	72.38	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold97...	[more]
A0A5A7SJH3	2.6e-117	67.44	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41...	[more]
A0A5A7UP12	2.8e-116	67.95	Ty3-gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of Cmc05g0140291.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 61..88 e-value: 4.0E-42 score: 145.9
IPR041373	Reverse transcriptase, RNase H-like domain	PFAM	PF17917	RT_RNaseH	coord: 164..252 e-value: 3.3E-29 score: 101.3
None	No IPR available	GENE3D	3.10.20.370		coord: 161..231 e-value: 3.7E-8 score: 35.2
None	No IPR available	GENE3D	3.10.10.10	HIV Type 1 Reverse Transcriptase, subunit A, domain 1	coord: 1..99 e-value: 4.0E-42 score: 145.9
None	No IPR available	PANTHER	PTHR24559:SF355	PROTEIN, PUTATIVE-RELATED	coord: 124..252
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 124..252
None	No IPR available	CDD	cd09274	RNase_HI_RT_Ty3	coord: 167..252 e-value: 1.20346E-40 score: 134.157
None	No IPR available	CDD	cd01647	RT_LTR	coord: 20..97 e-value: 1.10323E-38 score: 131.178
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 36..152 e-value: 4.4E-7 score: 29.7
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 2..252

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cmc05g0140291	Cmc05g0140291	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc05g0140291.1-exon	Cmc05g0140291.1-exon-CMiso1.1chr05:24737685..24738079	exon
Cmc05g0140291.1-exon	Cmc05g0140291.1-exon-CMiso1.1chr05:24738173..24738245	exon
Cmc05g0140291.1-exon	Cmc05g0140291.1-exon-CMiso1.1chr05:24738518..24738808	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc05g0140291.1-cds	Cmc05g0140291.1-cds-CMiso1.1chr05:24737685..24738079	CDS
Cmc05g0140291.1-cds	Cmc05g0140291.1-cds-CMiso1.1chr05:24738173..24738245	CDS
Cmc05g0140291.1-cds	Cmc05g0140291.1-cds-CMiso1.1chr05:24738518..24738808	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cmc05g0140291.1	Cmc05g0140291.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008233	peptidase activity
molecular_function	GO:0016740	transferase activity