Cmc04g0120161.1 (mRNA) Melon (Charmono) v1.1

Overview


Name	Cmc04g0120161.1
Type	mRNA
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Steroid 5-alpha-reductase DET2
Location	CMiso1.1chr04: 35856777 .. 35858404 (-)
Sequence length	1628
RNA-Seq Expression	Cmc04g0120161.1
Synteny	Cmc04g0120161.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGAAGAATAAAAAAAAGTGATTTTTTTTCCTTACTTGATTTTGAAATGTTCTTAACATTTTATATATTCAACATCTTTCCTCATCTATAAAAAGAAATACAAAAAAGAGAGAAACACCCAGTCATCTCTTTCCAAATTGAAAAAGGATAATTACTCTAATGAAAAATAGAGAATAAACCTTCTCCCCTTTTATTATATAGTTTTAGATCCATGGATTACATATTGAATAATGATTATTGATTTTCACCCTTTAATTTTACGGAACTTTATTAATCGAAATTACTCCACAAAGTCACCAAAAAGTGTCCCAAGTCTATTACCATAGTGGAGAAGGTGACTTCACTATGAGATTCAATAGGCTTCCTTCCCTCTGTCAGCTTCTTCCTTTTAGGTATGTAGATCTGGATTTTAATGAGATATGTTACAAATTTGAAAACAGATCAGCCACAAATTTCTCAACTTGATTCAAACCTACAACCGGAATCCTCGAATCCATCGCCTACCGTGATGGGTTGGGATCAGGCTCTGTTCCGCAACAGTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCTCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAGCACATCCGACCCGGGTGGGGTCCAACCATGTCGGCTCCATTGGCCTGGTTCCTGATGGAAAGTCCCACCTTATGGTTGACCCTCCTCCTCTTTCCATCCGGCGATCACTCCTCCGATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTTCACTACCTCAATCGCACCTGCTTCTACCCAATTCGTCTTCTCTGGAGTGGGACTCGACGGAATGCCACCGCCGGTTTTCCGGTGATTGTGGCTATCATGGCATTCGGATTCAACTTGCTCAATGCTTACGTGCAGACCAGATCTGTGTCTCATTACGTGAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTTGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAATATATGGTCGGATGGTGTTCTGGTCGGACTGAAGAGGAGCGGCGGCGGTTATAAGGTGCCGAAGGGAGGGTGGTTTGAGTTGGTGAGTTGTCCTAACTATTTGGGAGAGATTATGGAGTGGCTGGGCTGGGGGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTCCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCAAACCACACGTGGTACTTGAACAAGTTTGGAGAGGATTACCCCATAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAACTCTATGTCTATCATATTTCATATTAAAATAAAATAAATTCCTTTTGCCTTGTTTTAAAAGGGAATTTTTTTTTATATTCAAATACATTTTTTAGTGTTCAATAAGTCAAAAAATTACTAAGTAAGGCTTTTTTTTTTTAATTTGATGAAACATATTAACCATTTAATTATATTTACAAAATTCACAATTCATTTATATTTTCTTATTTCTAACAATGAGAAAAAAACATATATTTAAATTACATAATGTATCAAAGGCGGGGATAGCCGTCGTGGGAGAGCGTCAGGCGTGTTCGAATATAATAGGCAAGCAAGTAGCGACTCTCTCTTCGTTCTTCTC

mRNA sequence

GTGAAGAATAAAAAAAAGTGATTTTTTTTCCTTACTTGATTTTGAAATGTTCTTAACATTTTATATATTCAACATCTTTCCTCATCTATAAAAAGAAATACAAAAAAGAGAGAAACACCCAGTCATCTCTTTCCAAATTGAAAAAGGATAATTACTCTAATGAAAAATAGAGAATAAACCTTCTCCCCTTTTATTATATAGTTTTAGATCCATGGATTACATATTGAATAATGATTATTGATTTTCACCCTTTAATTTTACGGAACTTTATTAATCGAAATTACTCCACAAAGTCACCAAAAAGTGTCCCAAGTCTATTACCATAGTGGAGAAGGTGACTTCACTATGAGATTCAATAGGCTTCCTTCCCTCTGTCAGCTTCTTCCTTTTAGGTATGTAGATCTGGATTTTAATGAGATATGTTACAAATTTGAAAACAGATCAGCCACAAATTTCTCAACTTGATTCAAACCTACAACCGGAATCCTCGAATCCATCGCCTACCGTGATGGGTTGGGATCAGGCTCTGTTCCGCAACAGTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCTCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAGCACATCCGACCCGGGTGGGGTCCAACCATGTCGGCTCCATTGGCCTGGTTCCTGATGGAAAGTCCCACCTTATGGTTGACCCTCCTCCTCTTTCCATCCGGCGATCACTCCTCCGATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTTCACTACCTCAATCGCACCTGCTTCTACCCAATTCGTCTTCTCTGGAGTGGGACTCGACGGAATGCCACCGCCGGTTTTCCGGTGATTGTGGCTATCATGGCATTCGGATTCAACTTGCTCAATGCTTACGTGCAGACCAGATCTGTGTCTCATTACGTGAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTTGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAATATATGGTCGGATGGTGTTCTGGTCGGACTGAAGAGGAGCGGCGGCGGTTATAAGGTGCCGAAGGGAGGGTGGTTTGAGTTGGTGAGTTGTCCTAACTATTTGGGAGAGATTATGGAGTGGCTGGGCTGGGGGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTCCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCAAACCACACGTGGTACTTGAACAAGTTTGGAGAGGATTACCCCATAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAACTCTATGTCTATCATATTTCATATTAAAATAAAATAAATTCCTTTTGCCTTGTTTTAAAAGGGAATTTTTTTTTATATTCAAATACATTTTTTAGTGTTCAATAAGTCAAAAAATTACTAAGTAAGGCTTTTTTTTTTTAATTTGATGAAACATATTAACCATTTAATTATATTTACAAAATTCACAATTCATTTATATTTTCTTATTTCTAACAATGAGAAAAAAACATATATTTAAATTACATAATGTATCAAAGGCGGGGATAGCCGTCGTGGGAGAGCGTCAGGCGTGTTCGAATATAATAGGCAAGCAAGTAGCGACTCTCTCTTCGTTCTTCTC

Coding sequence (CDS)

ATGAGATATGTTACAAATTTGAAAACAGATCAGCCACAAATTTCTCAACTTGATTCAAACCTACAACCGGAATCCTCGAATCCATCGCCTACCGTGATGGGTTGGGATCAGGCTCTGTTCCGCAACAGTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCTCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAGCACATCCGACCCGGGTGGGGTCCAACCATGTCGGCTCCATTGGCCTGGTTCCTGATGGAAAGTCCCACCTTATGGTTGACCCTCCTCCTCTTTCCATCCGGCGATCACTCCTCCGATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTTCACTACCTCAATCGCACCTGCTTCTACCCAATTCGTCTTCTCTGGAGTGGGACTCGACGGAATGCCACCGCCGGTTTTCCGGTGATTGTGGCTATCATGGCATTCGGATTCAACTTGCTCAATGCTTACGTGCAGACCAGATCTGTGTCTCATTACGTGAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTTGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAATATATGGTCGGATGGTGTTCTGGTCGGACTGAAGAGGAGCGGCGGCGGTTATAAGGTGCCGAAGGGAGGGTGGTTTGAGTTGGTGAGTTGTCCTAACTATTTGGGAGAGATTATGGAGTGGCTGGGCTGGGGGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTCCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCAAACCACACGTGGTACTTGAACAAGTTTGGAGAGGATTACCCCATAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAA

Protein sequence

MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY

Homology

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BLAST of Cmc04g0120161.1 vs. NCBI nr
Match: XP_008442471.1 (PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo])

HSP 1 Score: 619.4 bits (1596), Expect = 1.6e-173
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0


Query: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60
           MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF
Sbjct: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 292
           GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY
Sbjct: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of Cmc04g0120161.1 vs. NCBI nr
Match: XP_004137737.1 (steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protein Csa_002591 [Cucumis sativus])

HSP 1 Score: 590.9 bits (1522), Expect = 6.1e-165
Identity = 277/291 (95.19%), Postives = 281/291 (96.56%), Query Frame = 0


Query: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60
           MR+ TNLKTD PQISQLDSNLQPESSNPSPT+MGWDQALF NSLLALYLIGPPTVVSLRF
Sbjct: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNA  GFPV+VAIMAFGFNLLN+YVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKR GGGYKVP GGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 292
            VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGE YPINRKAVIPFLY
Sbjct: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of Cmc04g0120161.1 vs. NCBI nr
Match: KAA0044147.1 (steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa])

HSP 1 Score: 557.8 bits (1436), Expect = 5.7e-155
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0


Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYPINRKAVIPFLY 292
           NKFGEDYPINRKAVIPFLY
Sbjct: 241 NKFGEDYPINRKAVIPFLY 259

[Close]

BLAST of Cmc04g0120161.1 vs. NCBI nr
Match: XP_038905208.1 (LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida])

HSP 1 Score: 498.0 bits (1281), Expect = 5.3e-137
Identity = 230/259 (88.80%), Postives = 242/259 (93.44%), Query Frame = 0


Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           MG DQALF NSLL LYLIGP  VV+LRFLQAPYGKH +PGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGSDQALFHNSLLRLYLIGPRQVVALRFLQAPYGKHNQPGWGPTMSAPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGDHSS+SKALLLISPFLLHY NRTC YPIRLL SGTRRN  AGFPVIVA+MA
Sbjct: 61  LTLLLFPSGDHSSNSKALLLISPFLLHYFNRTCLYPIRLLRSGTRRNTAAGFPVIVAVMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           FGFNLLN+Y+QTRSVSHY++YEGDGWFWWRFFGGGVVF++GM+VNIWSDGVLVGLKR GG
Sbjct: 121 FGFNLLNSYLQTRSVSHYMEYEGDGWFWWRFFGGGVVFLLGMSVNIWSDGVLVGLKRGGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVP G WFELVSCPNYLGEI+EWLGW VMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGWWFELVSCPNYLGEIVEWLGWAVMCGSWAGLAFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYPINRKAVIPFLY 292
           NKFGEDYP NRKAVIPFLY
Sbjct: 241 NKFGEDYPRNRKAVIPFLY 259

[Close]

BLAST of Cmc04g0120161.1 vs. NCBI nr
Match: XP_023527474.1 (steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 482.6 bits (1241), Expect = 2.3e-132
Identity = 224/259 (86.49%), Postives = 237/259 (91.51%), Query Frame = 0


Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LF NSLLALYLIGPPT VSLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAVSLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGD+SS+ K+LLLIS FLLHYLNRTC YPIRLL SGTRRN+  GFPV+VA+MA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           FGFNLLN+Y+QTRSVSHYV Y+ D WFWWRFFGG VVFV+GMAVNIWSDGVLV LK +GG
Sbjct: 121 FGFNLLNSYLQTRSVSHYVDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVP GGWFELVSCPNYLGEI+EWLGW VMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYPINRKAVIPFLY 292
           NKFGEDYP +RKAVIPFLY
Sbjct: 241 NKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of Cmc04g0120161.1 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.0e-98
Identity = 175/261 (67.05%), Postives = 197/261 (75.48%), Query Frame = 0


Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LF   LL LY+I  PT +SL FLQAPYGKH RPGWGPT+S PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LT  LFPSG H  + K+ LLISPFL HY NRT  YP+RL  + T+   T GFPV VA MA
Sbjct: 61  LTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRLARNTTQ---TRGFPVSVAFMA 120

Query: 153 FGFNLLNAYVQTRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSG 212
           FGFNLLN Y+Q R VSHY   YE +  FWWRF  G ++FV+GM VN+ +D VLVGLK+ G
Sbjct: 121 FGFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQG 180

Query: 213 -GGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTW 272
            GGYK+P+GG FELVSCPNY GEIMEW GW VM  SW G GFFLYTCANL+PRARA   W
Sbjct: 181 DGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLW 240

Query: 273 YLNKFGEDYPINRKAVIPFLY 292
           YL KF +DYP +RKAVIPF+Y
Sbjct: 241 YLEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of Cmc04g0120161.1 vs. ExPASy Swiss-Prot
Match: Q38944 (Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2)

HSP 1 Score: 346.7 bits (888), Expect = 2.6e-94
Identity = 163/258 (63.18%), Postives = 191/258 (74.03%), Query Frame = 0


Query: 36  DQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
           D+  FR  LL L   GPPT V L+FLQAPYGKH R GWGPT+S P+AWF+MESPTLWLTL
Sbjct: 6   DKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTL 65

Query: 96  LLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGF 155
           LLFP G H+ + K+LLL SP+L+HY +RT  YP+RL  S        GFP+ +A +AF F
Sbjct: 66  LLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKN-GFPITIAALAFTF 125

Query: 156 NLLNAYVQTRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSG-GG 215
           NLLN Y+Q R VSHY   YE   WFWWRF  G VVF+ GM +NI SD  LV LK+   GG
Sbjct: 126 NLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENRGG 185

Query: 216 YKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLN 275
           Y +P+GGWFELVSCPNY GE +EWLGW VM  SWAG+GFFLYTC+NL PRARA+H WY+ 
Sbjct: 186 YVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWYIA 245

Query: 276 KFGEDYPINRKAVIPFLY 292
           KF E+YP  RKAVIPF+Y
Sbjct: 246 KFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of Cmc04g0120161.1 vs. ExPASy Swiss-Prot
Match: Q5K2N1 (Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.2e-84
Identity = 149/257 (57.98%), Postives = 184/257 (71.60%), Query Frame = 0


Query: 36  DQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPG-WGPTMSAPLAWFLMESPTLWLT 95
           D+ LF   +  + ++  PT +  +F  +PYGKH      G T+S P+AW  MESPTLWLT
Sbjct: 5   DENLFNFIVFFILVMAFPTFILCQFFTSPYGKHYTSADSGTTISPPIAWAFMESPTLWLT 64

Query: 96  LLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFG 155
           +++F  G + ++  A LLISP+L HY NRT  YP+RL    T+ N    FP+ +A+ AF 
Sbjct: 65  IIVFRLGKNYTNPLAFLLISPYLFHYTNRTIIYPLRLRSRNTKNN----FPLNIAVTAFI 124

Query: 156 FNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGY 215
           FNLLNAY+Q+R VSHY  Y+ D WFW RF  G V+F  GM +NIW+DGVL+GLK  GGGY
Sbjct: 125 FNLLNAYIQSRWVSHYANYQEDDWFWVRFGIGLVIFGSGMLLNIWADGVLLGLKSQGGGY 184

Query: 216 KVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLNK 275
           K+P+GG F+ VS PNYLGEIMEWLGW +M  SWAGL FF+YTCANLVPRA +NH WYL K
Sbjct: 185 KIPRGGLFDYVSSPNYLGEIMEWLGWALMTWSWAGLAFFVYTCANLVPRAVSNHKWYLQK 244

Query: 276 FGEDYPINRKAVIPFLY 292
           FGEDYP NRKAV PFLY
Sbjct: 245 FGEDYPKNRKAVFPFLY 257

[Close]

BLAST of Cmc04g0120161.1 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-34
Identity = 89/243 (36.63%), Postives = 140/243 (57.61%), Query Frame = 0


Query: 54  TVVSLRFLQAPYGKHIRPGW-GPTMSAPLAWFLMESPTL-W-LTLLLFPSGDHSSDSKAL 113
           ++V LR + +PYG++  P W G  + A  AWF+ E P++ W L   + P+     +    
Sbjct: 27  SIVGLRSVGSPYGRY-SPQWPGIRVPARPAWFIQELPSMAWPLYEYIRPAAARLGNLPNR 86

Query: 114 LLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVA--IMAFGFNLLNAYVQTRSVS 173
           +L++ FL+HY+ RT  +P+ +           G P ++   ++AF F   N YVQ+R +S
Sbjct: 87  VLLAMFLIHYVQRTLVFPVLI---------RGGKPTLLVTFVLAFLFCTFNGYVQSRYLS 146

Query: 174 HYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSG-GGYKVPKGGWFELVSC 233
            +  Y  D      F  G  ++++GM +NI SD +L  L++ G  GYK+P+GG FE VS 
Sbjct: 147 QFAVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSA 206

Query: 234 PNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVI 291
            NY GE++EW G+ +   S  G+ F L+T + L+ RA+ +H WY  KF EDYP +RK +I
Sbjct: 207 ANYFGELVEWCGFALASWSLQGVVFALFTLSTLLTRAKQHHQWYHEKF-EDYPKSRKILI 258

[Close]

BLAST of Cmc04g0120161.1 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 6.8e-34
Identity = 89/240 (37.08%), Postives = 134/240 (55.83%), Query Frame = 0


Query: 56  VSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTL-W-LTLLLFPSGDHSSDSKALLLI 115
           V L+ + + YG++     G  + A  AWFL E P++ W L   + P+     +    +L+
Sbjct: 25  VGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLL 84

Query: 116 SPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIV--AIMAFGFNLLNAYVQTRSVSHYV 175
           + FL+HY+ RT  +P+ +           G P ++   ++AF F  LN Y+Q+R +S + 
Sbjct: 85  AMFLIHYVQRTLVFPVLI---------RGGKPTLLFTFVLAFLFCTLNGYLQSRYLSQFA 144

Query: 176 KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSG-GGYKVPKGGWFELVSCPNY 235
            Y  D      F  G  ++++GM +NI SD +L  L++ G  GYK+P+GG FE VS  NY
Sbjct: 145 VYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANY 204

Query: 236 LGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFL 291
            GE++EW G+ +   S  G+ F L+T   L  RAR +H WYL KF EDYP  RK +IPFL
Sbjct: 205 FGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKF-EDYPKTRKILIPFL 254

[Close]

BLAST of Cmc04g0120161.1 vs. ExPASy TrEMBL
Match: A0A1S3B6E3 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 7.7e-174
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0


Query: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60
           MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF
Sbjct: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 292
           GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY
Sbjct: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of Cmc04g0120161.1 vs. ExPASy TrEMBL
Match: A0A0A0LAB3 (Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 2.9e-165
Identity = 277/291 (95.19%), Postives = 281/291 (96.56%), Query Frame = 0


Query: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60
           MR+ TNLKTD PQISQLDSNLQPESSNPSPT+MGWDQALF NSLLALYLIGPPTVVSLRF
Sbjct: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNA  GFPV+VAIMAFGFNLLN+YVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKR GGGYKVP GGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 292
            VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGE YPINRKAVIPFLY
Sbjct: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of Cmc04g0120161.1 vs. ExPASy TrEMBL
Match: A0A5D3DNY2 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001080 PE=3 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 2.8e-155
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0


Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYPINRKAVIPFLY 292
           NKFGEDYPINRKAVIPFLY
Sbjct: 241 NKFGEDYPINRKAVIPFLY 259

[Close]

BLAST of Cmc04g0120161.1 vs. ExPASy TrEMBL
Match: A0A6J1J7F2 (Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.6e-131
Identity = 221/259 (85.33%), Postives = 236/259 (91.12%), Query Frame = 0


Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LF NSLLALYLIGPPT +SLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAISLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGD+SS+ K+LLLIS FLLHYLNRTC YPIRLL SGTRRN+  GFPV+VA+MA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           F FNLLN+Y+QTRSVSHY+ Y+ D WFWWRFFGG VVFV+GMAVNIWSDGVLV LK +GG
Sbjct: 121 FVFNLLNSYLQTRSVSHYMDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVP GGWFELVSCPNYLGEI+EWLGW VMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYPINRKAVIPFLY 292
           NKFGEDYP +RKAVIPFLY
Sbjct: 241 NKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of Cmc04g0120161.1 vs. ExPASy TrEMBL
Match: A0A6J1F1U8 (Steroid 5-alpha-reductase DET2 OS=Cucurbita moschata OX=3662 GN=LOC111441400 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.3e-130
Identity = 220/259 (84.94%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LF NSLLALYLIGPPT VSLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAVSLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGD+SS+ K+LLLIS FLLHYLNRTC YPIRLL SGTRRN+  GFPV++A+MA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVMALMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           F FNLLN+Y+QTRSVSHY+ Y+ D WFWWRFFGG VVF++GMAVNIWSDGVLV LK +GG
Sbjct: 121 FVFNLLNSYLQTRSVSHYMDYDRDEWFWWRFFGGAVVFLLGMAVNIWSDGVLVKLKMAGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVP GGWFELVSCPNYLGEI EWLGW VMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIAEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYPINRKAVIPFLY 292
           NKFGEDYP +RKAVIPFLY
Sbjct: 241 NKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of Cmc04g0120161.1 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 346.7 bits (888), Expect = 1.9e-95
Identity = 163/258 (63.18%), Postives = 191/258 (74.03%), Query Frame = 0


Query: 36  DQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
           D+  FR  LL L   GPPT V L+FLQAPYGKH R GWGPT+S P+AWF+MESPTLWLTL
Sbjct: 6   DKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTL 65

Query: 96  LLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGF 155
           LLFP G H+ + K+LLL SP+L+HY +RT  YP+RL  S        GFP+ +A +AF F
Sbjct: 66  LLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKN-GFPITIAALAFTF 125

Query: 156 NLLNAYVQTRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSG-GG 215
           NLLN Y+Q R VSHY   YE   WFWWRF  G VVF+ GM +NI SD  LV LK+   GG
Sbjct: 126 NLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENRGG 185

Query: 216 YKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLN 275
           Y +P+GGWFELVSCPNY GE +EWLGW VM  SWAG+GFFLYTC+NL PRARA+H WY+ 
Sbjct: 186 YVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWYIA 245

Query: 276 KFGEDYPINRKAVIPFLY 292
           KF E+YP  RKAVIPF+Y
Sbjct: 246 KFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of Cmc04g0120161.1 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-14
Identity = 40/108 (37.04%), Postives = 69/108 (63.89%), Query Frame = 0


Query: 186 GGVVFVMGMAVNIWSDGVLVGLKRSGG--GYKVPKGGWFELVSCPNYLGEIMEWLGWGVM 245
           G V+FV+G+  N++   +L  L++  G   YK+PKGG F+++ CP+YL EI+ +  + ++
Sbjct: 162 GVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLI 221

Query: 246 CGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 292
             +     F + T   L+ R+ A  TWYL+KF +D+P + KA+IPF++
Sbjct: 222 SQTIYSFSFAMGTMLYLIGRSYATRTWYLSKF-DDFPKHIKALIPFVF 268

[Close]

BLAST of Cmc04g0120161.1 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-12
Identity = 42/114 (36.84%), Postives = 57/114 (50.00%), Query Frame = 0


Query: 186 GGVVFVMGMAVNIWSDGVLVGLKR---SGGGYKVPKGGWFELVSCPNYLGEIMEWLGWGV 245
           GG +F+ G         +L  L+        Y +P G WFE+VSCP++L EI+ +LG  +
Sbjct: 211 GGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLI 270

Query: 246 MCGS-----WAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 292
             G      W   GF     ANL   A   H WYL KF E+YP +R A+ P +Y
Sbjct: 271 SSGGTDISIWLLFGF---VAANLTYAAGETHRWYLQKF-ENYPASRHAIFPHVY 320

[Close]

BLAST of Cmc04g0120161.1 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-11
Identity = 48/145 (33.10%), Postives = 67/145 (46.21%), Query Frame = 0


Query: 155 FNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRS---G 214
           FNLL+      S+S  +K     W       GG +F+ G         +L  L+ +    
Sbjct: 215 FNLLS------SISPLMKLGSLQWI------GGAIFLWGWIHQRRCHAILGSLRENPSQA 274

Query: 215 GGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGS-----WAGLGFFLYTCANLVPRARA 274
             Y +P G WF +VS P++L EI+ + G  +  G      W   GF     ANL   A  
Sbjct: 275 KEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF---VAANLTYAAGE 334

Query: 275 NHTWYLNKFGEDYPINRKAVIPFLY 292
            H WYL KF E+YP NR A+ P++Y
Sbjct: 335 THRWYLRKF-ENYPANRHAIFPYVY 343

[Close]

BLAST of Cmc04g0120161.1 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-11
Identity = 48/145 (33.10%), Postives = 67/145 (46.21%), Query Frame = 0


Query: 155 FNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRS---G 214
           FNLL+      S+S  +K     W       GG +F+ G         +L  L+ +    
Sbjct: 214 FNLLS------SISPLMKLGSLQWI------GGAIFLWGWIHQRRCHAILGSLRENPSQA 273

Query: 215 GGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGS-----WAGLGFFLYTCANLVPRARA 274
             Y +P G WF +VS P++L EI+ + G  +  G      W   GF     ANL   A  
Sbjct: 274 KEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF---VAANLTYAAGE 333

Query: 275 NHTWYLNKFGEDYPINRKAVIPFLY 292
            H WYL KF E+YP NR A+ P++Y
Sbjct: 334 THRWYLRKF-ENYPANRHAIFPYVY 342

The following BLAST results are available for this feature:

BLAST of Cmc04g0120161.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442471.1	1.6e-173	100.00	PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo]	[more]
XP_004137737.1	6.1e-165	95.19	steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protei...	[more]
KAA0044147.1	5.7e-155	100.00	steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-...	[more]
XP_038905208.1	5.3e-137	88.80	LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida]	[more]
XP_023527474.1	2.3e-132	86.49	steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cmc04g0120161.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QDF6	1.0e-98	67.05	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
Q38944	2.6e-94	63.18	Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2	[more]
Q5K2N1	4.2e-84	57.98	Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1	[more]
P24008	1.4e-34	36.63	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]
Q68FF9	6.8e-34	37.08	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]

BLAST of Cmc04g0120161.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B6E3	7.7e-174	100.00	Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1	[more]
A0A0A0LAB3	2.9e-165	95.19	Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 S...	[more]
A0A5D3DNY2	2.8e-155	100.00	Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1J7F2	3.6e-131	85.33	Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 ...	[more]
A0A6J1F1U8	2.3e-130	84.94	Steroid 5-alpha-reductase DET2 OS=Cucurbita moschata OX=3662 GN=LOC111441400 PE=...	[more]

BLAST of Cmc04g0120161.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38050.1	1.9e-95	63.18	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	1.2e-14	37.04	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	2.0e-12	36.84	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	1.7e-11	33.10	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.2	1.7e-11	33.10	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 144..291 e-value: 1.8E-46 score: 157.8
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 186..265 score: 26.181702
None	No IPR available	GENE3D	1.20.120.1630		coord: 108..290 e-value: 3.8E-11 score: 45.1
None	No IPR available	PANTHER	PTHR10556:SF53	STEROID 5-ALPHA-REDUCTASE DET2	coord: 35..291
IPR016636	3-oxo-5-alpha-steroid 4-dehydrogenase	PIRSF	PIRSF015596	5_alpha-SR2	coord: 31..291 e-value: 8.0E-84 score: 278.9
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 35..291

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cmc04g0120161	Cmc04g0120161	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc04g0120161.1-exon	Cmc04g0120161.1-exon-CMiso1.1chr04:35856777..35858404	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc04g0120161.1-three_prime_utr	Cmc04g0120161.1-three_prime_utr-CMiso1.1chr04:35856777..35857116	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc04g0120161.1-cds	Cmc04g0120161.1-cds-CMiso1.1chr04:35857117..35857992	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc04g0120161.1-five_prime_utr	Cmc04g0120161.1-five_prime_utr-CMiso1.1chr04:35857993..35858404	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cmc04g0120161.1	Cmc04g0120161.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0008202	steroid metabolic process
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
cellular_component	GO:0016020	membrane
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors
molecular_function	GO:0047751	cholestenone 5-alpha-reductase activity
molecular_function	GO:0050213	progesterone 5-alpha-reductase activity
molecular_function	GO:0009917	sterol 5-alpha reductase activity