CmaCh16G001060.1 (mRNA) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh16G001060.1 vs. ExPASy Swiss-Prot
Match: Q8W110 (Ferric reduction oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=FRO4 PE=2 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 4.2e-223
Identity = 381/699 (54.51%), Postives = 517/699 (73.96%), Query Frame = 0

Query: 11  VKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLLFSFPVMLMAV 70
           VK + ++ F+ W+ +WIM+ T L+K+ WTPKL+  LN+TYF  QGTNL+L + P+M +AV
Sbjct: 8   VKTLMVVLFLGWILVWIMISTNLFKSKWTPKLSKYLNTTYFGPQGTNLVLLTVPMMFIAV 67

Query: 71  LGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGFAAAFVILLIW 130
           L C YL +QK  ++  + +  +R  G     R ++ MNPLGI+   E+ F+  FV LL W
Sbjct: 68  LSCVYLHIQKKPTQPQREWKLKRIMG-----RVIMVMNPLGIVTATELTFSLLFVALLAW 127

Query: 131 SLGNYLYVSFGNLHMH-VAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSSLLPII 190
           SL NYLY+S+ ++H+H     K+W+AKFR+  LR+GY+G+ICWAFLFFPVTR S++LP++
Sbjct: 128 SLYNYLYLSY-HVHLHNDDNAKIWQAKFRAFGLRIGYVGNICWAFLFFPVTRASTILPLV 187

Query: 191 GLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAGLIAMA 250
           GLTSES+IKYHIWLGH+SN  F +HTV F+IYW + +++     W+  YV N+AG IAM 
Sbjct: 188 GLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMINKLMETFAWNPTYVPNLAGTIAMV 247

Query: 251 FATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGMFLFAIDRFL 310
               +WVTS  S RRK FE+FFY+HH Y LY VFY+IHVG ++ C+ILP +FLF IDR+L
Sbjct: 248 IGIAMWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYVIHVGDSWFCMILPNIFLFFIDRYL 307

Query: 311 RFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQWHPFTITSN 370
           RFLQS +R  LVSAR LP   +EL   K+  L Y+PTS++FL+VP+ISK+QWHPFTITS+
Sbjct: 308 RFLQSTKRSRLVSARILPSDNLELTFSKTPGLHYTPTSILFLHVPSISKIQWHPFTITSS 367

Query: 371 SKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRHESLLLISGG 430
           S LE D +SV I+  GSWT KL+  LSSS+D L+VS EGPYGP S    RH SL+L+SGG
Sbjct: 368 SNLEKDTLSVVIRRQGSWTQKLYTHLSSSIDSLEVSTEGPYGPNSFDVSRHNSLILVSGG 427

Query: 431 SGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQTEISNVP 490
           SGITPFIS+IRE +I +S   +  +P V LVC+FK+  DL  LDL+ P+  + ++IS + 
Sbjct: 428 SGITPFISVIRE-LISQSQNKSTKLPDVLLVCSFKHYHDLAFLDLIFPLDMSASDISRLN 487

Query: 491 LQIDAYITREEEQPQTNEEN-LINTIWFKPNPMDSPVSEALGPNNWLLLGTVISSSFVMF 550
           L+I+AYITRE+++P+T +++ L+ T WFKP P+DSP+S  LGPNN+L LG VI SSFVMF
Sbjct: 488 LRIEAYITREDKKPETTDDHRLLQTKWFKPQPLDSPISPVLGPNNFLWLGVVILSSFVMF 547

Query: 551 LILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRKTYVS--- 610
           L+L+GI+TRYYIYP++ N  ++YN++Y+ LWDMFL  ACI I SS+V +W++K       
Sbjct: 548 LLLIGIVTRYYIYPVDHNTGSIYNFSYRGLWDMFLGSACIFISSSVVFLWRKKQNKEGDK 607

Query: 611 --NKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLDCKG 670
               QVQ +  Q+P +SPGSW  G ERELES+PYQ +VQAT++H+G +P+LK+ILL+ +G
Sbjct: 608 EFKNQVQSVEFQTPTSSPGSWFHGHERELESVPYQSIVQATSVHFGSKPNLKKILLEAEG 667

Query: 671 S-DIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           S D+GV+V GP  MRH+VAKICS+    NL+F A+SF W
Sbjct: 668 SEDVGVMVCGPRKMRHEVAKICSSGLAKNLHFEAISFNW 699

BLAST of CmaCh16G001060.1 vs. ExPASy Swiss-Prot
Match: Q9FLW2 (Ferric reduction oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=FRO5 PE=2 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 6.1e-222
Identity = 378/701 (53.92%), Postives = 510/701 (72.75%), Query Frame = 0

Query: 11  VKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLLFSFPVMLMAV 70
           VKM+ ++ F+ W+++WIM+ T  ++N WTPKL   L +TYF  QG NL+L + P+M +AV
Sbjct: 8   VKMLMVVLFLGWIFVWIMISTNRFQNIWTPKLAKYLKTTYFGPQGMNLVLLTVPMMFIAV 67

Query: 71  LGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGFAAAFVILLIW 130
           L C YL  QK  S+    +  +  +    + R ++ MNPLGI+   E+ F+  F+ LL+W
Sbjct: 68  LSCVYLHTQKQPSQTQSLYKCREWKVKGRMGRVMMVMNPLGIVTATELTFSLLFLALLVW 127

Query: 131 SLGNYLYVSFG-NLHMHVAGEKV--WEAKFRSVSLRLGYIGHICWAFLFFPVTRGSSLLP 190
           +L NYLY+S+  +LH H     +  W+AKFR+  LR+GY+GH CWAFLFFPVTR S++LP
Sbjct: 128 ALSNYLYLSYHVHLHNHDNANDMCRWQAKFRAFGLRIGYVGHYCWAFLFFPVTRASTILP 187

Query: 191 IIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAGLIA 250
           ++GLTSES+IKYHIWLGH+SN  F +HTV F+IYW + +++     W+  YV N+AG IA
Sbjct: 188 LVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMVNKLMETFAWNATYVPNLAGTIA 247

Query: 251 MAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGMFLFAIDR 310
           M     IWVTS  S RRK FE+FFY+HH Y LY VFY IHVG ++ C+ILP +FLF IDR
Sbjct: 248 MVIGIAIWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYAIHVGDSWFCMILPNIFLFFIDR 307

Query: 311 FLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQWHPFTIT 370
           +LRFLQS +R  LVSA+ LP   +EL   K+S L Y+PTS++FL+VP+ISKLQWHPFTIT
Sbjct: 308 YLRFLQSTKRSRLVSAKILPSDNLELTFAKTSGLHYTPTSILFLHVPSISKLQWHPFTIT 367

Query: 371 SNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRHESLLLIS 430
           S+S LE D +SV I+  GSWT KL+  LSSS+D L+VS EGPYGP S    RH+SL+L+ 
Sbjct: 368 SSSNLEKDTLSVVIRKQGSWTQKLYTHLSSSIDSLEVSTEGPYGPNSFDVSRHDSLILVG 427

Query: 431 GGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQTEISN 490
           GGSG+TPFIS+IRE +I +S   +  +P V LVCAFKN  DL  LDL+ P   + ++IS 
Sbjct: 428 GGSGVTPFISVIRE-LIFQSQNRSTKLPNVLLVCAFKNYHDLAFLDLIFPSDISVSDISK 487

Query: 491 VPLQIDAYITREEEQPQTNEEN-LINTIWFKPNPMDSPVSEALGPNNWLLLGTVISSSFV 550
           + L+I+AYITRE+++P+T +++ L+ T WFKP P+DSP+S  LGPNN+L LG VI SSFV
Sbjct: 488 LNLRIEAYITREDKKPETTDDHKLLQTKWFKPQPLDSPISPVLGPNNFLWLGVVILSSFV 547

Query: 551 MFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRKTYV-- 610
           MFL+L+GI+TRYYIYP++ N  ++YN+TY+VLW MFL   CI I SS++ +W++K     
Sbjct: 548 MFLLLIGIVTRYYIYPVDHNTGSIYNFTYRVLWVMFLGCVCIFISSSIIFLWRKKENKEG 607

Query: 611 ---SNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLDC 670
              S KQVQ +  Q+P +SPGSW  G ERELES+PYQ +VQAT++H+G +P+LK+IL + 
Sbjct: 608 DKDSKKQVQSVEFQTPTSSPGSWFHGHERELESVPYQSIVQATSVHFGSKPNLKKILFEA 667

Query: 671 KGS-DIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           +GS D+GV+V GP  MRH+VAKICS+    NL+F A+SF W
Sbjct: 668 EGSEDVGVMVCGPKKMRHEVAKICSSGLAKNLHFEAISFNW 707

BLAST of CmaCh16G001060.1 vs. ExPASy Swiss-Prot
Match: P92949 (Ferric reduction oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=FRO2 PE=1 SV=2)

HSP 1 Score: 675.6 bits (1742), Expect = 5.9e-193
Identity = 346/708 (48.87%), Postives = 497/708 (70.20%), Query Frame = 0

Query: 7   MARGV-KMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDL-NSTYFREQGTNLLLFSFP 66
           M +GV K + ++ F+  + LWIM+PT  Y+  W P L +    STYF   GT L ++ FP
Sbjct: 22  MIKGVTKFLMMVIFLGTIMLWIMMPTLTYRTKWLPHLRIKFGTSTYFGATGTTLFMYMFP 81

Query: 67  VMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGD-SFLKRPVLAMNPLGILNTLEIGFAAA 126
           +M++A LGC YL  +   S  P     +   G  S L++P+L   PLGI++  EI F A 
Sbjct: 82  MMVVACLGCVYLHFKNRKS--PHHIDRETKGGVWSKLRKPMLVKGPLGIVSVTEITFLAM 141

Query: 127 FVILLIWSLGNYLYVSFGNLHMHVAG---EKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 186
           FV LL+W    YL  SF  +    A    E +W+AK  S +LRLG IG+IC AFLF PV 
Sbjct: 142 FVALLLWCFITYLRNSFATITPKSAAAHDESLWQAKLESAALRLGLIGNICLAFLFLPVA 201

Query: 187 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 246
           RGSSLLP +GLTSES+IKYHIWLGH+   LF +H + +IIYW    +++ +I W    VS
Sbjct: 202 RGSSLLPAMGLTSESSIKYHIWLGHMVMALFTVHGLCYIIYWASMHEISQMIMWDTKGVS 261

Query: 247 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 306
           N+AG IA+A   V+W T++  IRR+ FEVFFY+H+ Y ++ +F+++HVG ++  + LPG 
Sbjct: 262 NLAGEIALAAGLVMWATTYPKIRRRFFEVFFYTHYLYIVFMLFFVLHVGISFSFIALPGF 321

Query: 307 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 366
           ++F +DRFLRFLQS+  V L++AR LP   +EL   K+S+L YSPTS+MF+N+P+ISKLQ
Sbjct: 322 YIFLVDRFLRFLQSRENVRLLAARILPSDTMELTFSKNSKLVYSPTSIMFVNIPSISKLQ 381

Query: 367 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSS--LDHLQVSVEGPYGPASTRFL 426
           WHPFTITS+SKLEP+ +S+ IK  G W++KLHQ+LSSS  +D L VSVEGPYGPAS  FL
Sbjct: 382 WHPFTITSSSKLEPEKLSIVIKKEGKWSTKLHQRLSSSDQIDRLAVSVEGPYGPASADFL 441

Query: 427 RHESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPV 486
           RHE+L+++ GGSGITPFIS+IR+ +I  S K  C +P++ L+CAFK S +++MLDL+LP+
Sbjct: 442 RHEALVMVCGGSGITPFISVIRD-LIATSQKETCKIPKITLICAFKKSSEISMLDLVLPL 501

Query: 487 SSTQTEI-SNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLL 546
           S  +TE+ S++ ++I+A+ITR+ +     +   I T+WFKP+  D  +S  LGPN+WL L
Sbjct: 502 SGLETELSSDINIKIEAFITRDNDAGDEAKAGKIKTLWFKPSLSDQSISSILGPNSWLWL 561

Query: 547 GTVISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYK-VLWDMFLSLACICICSSLVL 606
           G +++SSF++F+I++GIITRYYIYPI+ N + +Y+ T K +++ + +S++ +  CS+  +
Sbjct: 562 GAILASSFLIFMIIIGIITRYYIYPIDHNTNKIYSLTSKTIIYILVISVSIMATCSA-AM 621

Query: 607 MWQRKTY--VSNKQVQVLNVQSPKASP-GSWIFGGERELESLPYQPLVQATNIHYGGRPD 666
           +W +K Y  V +KQVQ ++  SP +SP  SW +   RE+ES P + LVQ TN+H+G RP+
Sbjct: 622 LWNKKKYGKVESKQVQNVDRPSPTSSPTSSWGYNSLREIESTPQESLVQRTNLHFGERPN 681

Query: 667 LKRILLDCKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           LK++LLD +GS +GVLV GP  MR  VA+ICS+   +NL+F ++SF+W
Sbjct: 682 LKKLLLDVEGSSVGVLVCGPKKMRQKVAEICSSGLAENLHFESISFSW 725

BLAST of CmaCh16G001060.1 vs. ExPASy Swiss-Prot
Match: Q9LMM2 (Probable ferric reduction oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=2 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 1.5e-180
Identity = 327/705 (46.38%), Postives = 476/705 (67.52%), Query Frame = 0

Query: 11  VKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDL-NSTYFREQGTNLLLFSFPVMLMA 70
           +K + ++  +  + +WIM+PT  YK  W   +   L  S Y+   G NLL++ FP++L+A
Sbjct: 15  IKFLMMVILMGTIVIWIMMPTSTYKEIWLTSMRAKLGKSIYYGRPGVNLLVYMFPMILLA 74

Query: 71  VLGCFYLQLQKSSSEDPKPFSTQRSRGD-SFLKRPVLAMNPLGILNTLEIGFAAAFVILL 130
            LGC YL L+KS++ +      ++ R     L+RP+L   PLGI+   E+ F   F+ LL
Sbjct: 75  FLGCIYLHLKKSTTVNQFNSGVEKKRAKFGALRRPMLVNGPLGIVTVTEVMFLTMFMALL 134

Query: 131 IWSLGNYLYVSFGNLHMHVA---GEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSSL 190
           +WSL NY+Y +F N+    A   G  +W+A+   +++R+G +G+IC AFLF+PV RGSSL
Sbjct: 135 LWSLANYMYRTFVNVTSESAATDGNNLWQARLDLIAVRIGIVGNICLAFLFYPVARGSSL 194

Query: 191 LPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQM-ALLIEWSEDYVSNIAG 250
           L  +GLTSES+IKYHIWLGH+  ++F  H + + IYW   +Q+ + ++EW    VSN+AG
Sbjct: 195 LAAVGLTSESSIKYHIWLGHLVMIIFTSHGLCYFIYWISKNQLVSKMLEWDRTAVSNLAG 254

Query: 251 LIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGMFLFA 310
            IA+    ++WVT++  IRR++FEVFFYSH+ Y ++ +F++ HVG ++  + LPG ++F 
Sbjct: 255 EIALVAGLMMWVTTYPKIRRRLFEVFFYSHYLYIVFMLFFVFHVGISHALIPLPGFYIFL 314

Query: 311 IDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQWHPF 370
           +DRFLRFLQS+  V LVSAR LPC  VELN  K+  L YSPTS MF+N+P+ISKLQWHPF
Sbjct: 315 VDRFLRFLQSRNNVKLVSARVLPCDTVELNFSKNPMLMYSPTSTMFVNIPSISKLQWHPF 374

Query: 371 TITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSS----LDHLQVSVEGPYGPASTRFLRH 430
           TI S+SKLEP+ +SV IK  G W++KL+  LSSS    ++ L VSVEGPYGP+ST FLRH
Sbjct: 375 TIISSSKLEPETLSVMIKSQGKWSTKLYDMLSSSSSDQINRLAVSVEGPYGPSSTDFLRH 434

Query: 431 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 490
           ESL+++SGGSGITPFISI+R++  + ST   C +P++ L+CAFKNS DL+MLDL+LP S 
Sbjct: 435 ESLVMVSGGSGITPFISIVRDLFYMSSTH-KCKIPKMTLICAFKNSSDLSMLDLILPTSG 494

Query: 491 TQTEISN-VPLQIDAYITREEE---QPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLL 550
             T++++ V +QI A++TREE+   +  T+  N+I T  FKPN  D P+S  LGPN+WL 
Sbjct: 495 LTTDMASFVDIQIKAFVTREEKTSVKESTHNRNIIKTRHFKPNVSDQPISPILGPNSWLC 554

Query: 551 LGTVISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVL 610
           L  ++SSSF++F++++ IITRY+I+PI++N +  Y + YK L  +      +   S+  +
Sbjct: 555 LAAILSSSFMIFIVIIAIITRYHIHPIDQNSEK-YTWAYKSLIYLVSISITVVTTSTAAM 614

Query: 611 MWQRKTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKR 670
           +W +K Y +     V N+ SP              +ES P Q + Q+T+IHYG RP+L +
Sbjct: 615 LWNKKKYYAKNDQYVDNL-SPVI------------IESSPQQLISQSTDIHYGERPNLNK 674

Query: 671 ILLDCKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           +L+  KGS +G+LV GP  MR  VAKICS    +NL+F ++SF+W
Sbjct: 675 LLVGLKGSSVGILVCGPKKMRQKVAKICSFGSAENLHFESISFSW 704

BLAST of CmaCh16G001060.1 vs. ExPASy Swiss-Prot
Match: F4I4K7 (Ferric reduction oxidase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO3 PE=2 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 3.4e-172
Identity = 324/700 (46.29%), Postives = 468/700 (66.86%), Query Frame = 0

Query: 11  VKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDL-NSTYFREQGTNLLLFSFPVMLMA 70
           +K++ ++  +  + +WIM+PT  YK  W   +   L  S YF + G NLL++ FP++L+A
Sbjct: 38  IKLLTMVILMGTVVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFPMILLA 97

Query: 71  VLGCFYLQLQKSSSEDPKPFSTQRSRGDSF--LKRPVLAMNPLGILNTLEIGFAAAFVIL 130
            LG  YL L+K +  +       R + D F  LKRP+L    LGI+   E+ F   F+ L
Sbjct: 98  SLGSIYLHLKKQTRVNQFNSRMDRKKIDKFGALKRPMLVKAGLGIVTVTEVMFLMMFMAL 157

Query: 131 LIWSLGNYLYVSFGNL---HMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSS 190
           L+WSL NY Y +F  +    +   G+ +W+A+  S+++RLG  G+IC  FLF+PV RGSS
Sbjct: 158 LLWSLANYFYHTFVTITPQSLPTDGDNLWQARLDSIAVRLGLTGNICLGFLFYPVARGSS 217

Query: 191 LLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAG 250
           LL  +GLTSES+ KYHIWLG++   LF  H + + IYW  T+Q++ ++EW    +S++AG
Sbjct: 218 LLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQVSQMLEWDRTGISHLAG 277

Query: 251 LIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGMFLFA 310
            IA+    ++W T+F +IRR+ FEVFFY+H+ Y ++ +F++ HVG +Y  +  PG ++F 
Sbjct: 278 EIALVAGLLMWATTFPAIRRRFFEVFFYTHYLYMVFMLFFVFHVGISYALISFPGFYIFM 337

Query: 311 IDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQWHPF 370
           +DRFLRFLQS+  V LVSAR LPC  VELN  K+  L YSPTS++F+N+P+ISKLQWHPF
Sbjct: 338 VDRFLRFLQSRNNVKLVSARVLPCETVELNFSKNPMLMYSPTSILFVNIPSISKLQWHPF 397

Query: 371 TITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSS--LDHLQVSVEGPYGPASTRFLRHES 430
           TITS+SKLEP  +SV IK  G W+SKLH  L+SS  +DHL VSVEGPYGPAST +LRH+S
Sbjct: 398 TITSSSKLEPKKLSVMIKSQGKWSSKLHHMLASSNQIDHLAVSVEGPYGPASTDYLRHDS 457

Query: 431 LLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQ 490
           L+++SGGSGITPFISIIR+++ V ST      P++ L+CAFKNS DL+ML+L+LP S   
Sbjct: 458 LVMVSGGSGITPFISIIRDLLYVSSTNA-YKTPKITLICAFKNSSDLSMLNLILPNS--- 517

Query: 491 TEISN-VPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTVI 550
           TEIS+ + +QI A++TR  E+  T   N+I T+ FKP   D P+S  LGPN+WL L T++
Sbjct: 518 TEISSFIDIQIKAFVTR--EKVSTCNMNIIKTLSFKPYVSDQPISPILGPNSWLWLATIL 577

Query: 551 SSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRK 610
           SSSF++F+I++ II+RY+IYPI+++     +    +++ + +S++ +   S++ ++  +K
Sbjct: 578 SSSFMIFIIIIAIISRYHIYPIDQSSKEYTSAYTSLIYLLAISISVVA-TSTVAMLCNKK 637

Query: 611 TYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLDC 670
           +Y      Q ++  SP              +ES P Q L + TNIHYG RP+L ++L+  
Sbjct: 638 SYFKG-LYQNVDALSPLM------------IESSPDQLLPEFTNIHYGERPNLNKLLVGL 697

Query: 671 KGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           KGS +GVLV GP  MR +VAKICS     NL F ++SF W
Sbjct: 698 KGSSVGVLVCGPRKMREEVAKICSFGSAANLQFESISFNW 717

BLAST of CmaCh16G001060.1 vs. ExPASy TrEMBL
Match: A0A6J1JC03 (ferric reduction oxidase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483082 PE=4 SV=1)

HSP 1 Score: 1401.3 bits (3626), Expect = 0.0e+00
Identity = 701/701 (100.00%), Postives = 701/701 (100.00%), Query Frame = 0

Query: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60
           MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL
Sbjct: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60

Query: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120
           FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF
Sbjct: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120

Query: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180
           AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT
Sbjct: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180

Query: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240
           RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS
Sbjct: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240

Query: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300
           NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM
Sbjct: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300

Query: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360
           FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ
Sbjct: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360

Query: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420
           WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH
Sbjct: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420

Query: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480
           ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS
Sbjct: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480

Query: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540
           TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV
Sbjct: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540

Query: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600
           ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR
Sbjct: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600

Query: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660
           KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD
Sbjct: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660

Query: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW
Sbjct: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 701

BLAST of CmaCh16G001060.1 vs. ExPASy TrEMBL
Match: A0A6J1E4P2 (ferric reduction oxidase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111430749 PE=4 SV=1)

HSP 1 Score: 1382.1 bits (3576), Expect = 0.0e+00
Identity = 689/701 (98.29%), Postives = 694/701 (99.00%), Query Frame = 0

Query: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60
           MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL
Sbjct: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60

Query: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120
           FSFPVMLMAVLGCFYL LQKSSSEDPKPFSTQRSRGDSFLKRPVL MNPLGILNTLEIGF
Sbjct: 61  FSFPVMLMAVLGCFYLHLQKSSSEDPKPFSTQRSRGDSFLKRPVLVMNPLGILNTLEIGF 120

Query: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180
           AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT
Sbjct: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180

Query: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240
           RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS
Sbjct: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240

Query: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300
           NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCL+LPGM
Sbjct: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLVLPGM 300

Query: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360
           FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKS ELSYSPTSVMFLNVPTISKLQ
Sbjct: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSPELSYSPTSVMFLNVPTISKLQ 360

Query: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420
           WHPFT+TSNSKLEP IISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPAST FLRH
Sbjct: 361 WHPFTVTSNSKLEPAIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRH 420

Query: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480
           ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS
Sbjct: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480

Query: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540
           TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNP+DSPV+EALGPNNWLLLG V
Sbjct: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPLDSPVTEALGPNNWLLLGAV 540

Query: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600
           ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR
Sbjct: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600

Query: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660
           KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLP+QPLVQATNIHYGGRPDLKRILLD
Sbjct: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPFQPLVQATNIHYGGRPDLKRILLD 660

Query: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           CKGSDIGVLVSGP GMRHDVAKICSTAYGDNLNFHAMSFTW
Sbjct: 661 CKGSDIGVLVSGPTGMRHDVAKICSTAYGDNLNFHAMSFTW 701

BLAST of CmaCh16G001060.1 vs. ExPASy TrEMBL
Match: A0A6J1E5E9 (ferric reduction oxidase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111430747 PE=4 SV=1)

HSP 1 Score: 1310.0 bits (3389), Expect = 0.0e+00
Identity = 645/701 (92.01%), Postives = 676/701 (96.43%), Query Frame = 0

Query: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60
           MAKEMAMAR +KM+FLLTFVLWLWLWIMLPTKLYKNTWTPKL M+LNSTYFREQGTNLLL
Sbjct: 1   MAKEMAMARALKMIFLLTFVLWLWLWIMLPTKLYKNTWTPKLKMELNSTYFREQGTNLLL 60

Query: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120
           FSFP+M MAVLGCFYL LQK+SSED KPFSTQRSRGDSFLKRP+LAMNPLGI+NTLEIGF
Sbjct: 61  FSFPIMFMAVLGCFYLHLQKNSSEDSKPFSTQRSRGDSFLKRPILAMNPLGIVNTLEIGF 120

Query: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180
           AAAFVILLIWSLGNYLYVSFGNLHMH AGEK+WEAKFRSVSLRLGYIGHICWAFLFFPVT
Sbjct: 121 AAAFVILLIWSLGNYLYVSFGNLHMHGAGEKIWEAKFRSVSLRLGYIGHICWAFLFFPVT 180

Query: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240
           RGSSLLP+IGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALL EWSE YVS
Sbjct: 181 RGSSLLPLIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLKEWSEVYVS 240

Query: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300
           N+AGLIAMAFATVIWVTSFRSIR+KMFEVFFYSHH YTLYTVFYLIHVGAAYVC+ILPGM
Sbjct: 241 NVAGLIAMAFATVIWVTSFRSIRQKMFEVFFYSHHLYTLYTVFYLIHVGAAYVCMILPGM 300

Query: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360
           FLFAIDRFLRFLQSQR VSLVSARHLPCG VELNLLKS ELSYSPTSVMFLNVP+ISKLQ
Sbjct: 301 FLFAIDRFLRFLQSQRHVSLVSARHLPCGVVELNLLKSPELSYSPTSVMFLNVPSISKLQ 360

Query: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420
           WHPFTITSNSKLEPDI+SV  KVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPAST FLRH
Sbjct: 361 WHPFTITSNSKLEPDIVSVVSKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRH 420

Query: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480
           ESLLLISGGSGITPFISIIREIMIVRSTKP+CHVPQVRL+CAFKNS+DLTMLDLLLPVSS
Sbjct: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPDCHVPQVRLICAFKNSMDLTMLDLLLPVSS 480

Query: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540
           TQTEISNVPLQI+AYITREEE PQTNEENL+NTIWFKPNP+DSPVSEALGPN+WLLLG V
Sbjct: 481 TQTEISNVPLQIEAYITREEEPPQTNEENLVNTIWFKPNPLDSPVSEALGPNHWLLLGAV 540

Query: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600
           ISSSF+MFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFL+LACIC+ SS VLMWQR
Sbjct: 541 ISSSFMMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLALACICVSSSFVLMWQR 600

Query: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660
           KTYVSNK+VQVL+VQSPKASPGSWI+GGERELESLPYQ LVQATN+HYG RPDLK+ILLD
Sbjct: 601 KTYVSNKRVQVLSVQSPKASPGSWIYGGERELESLPYQSLVQATNVHYGSRPDLKKILLD 660

Query: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           CKGSDIGVLVSGP  +RHDVAKICSTA GDNL FHA+SFTW
Sbjct: 661 CKGSDIGVLVSGPTRLRHDVAKICSTACGDNLKFHALSFTW 701

BLAST of CmaCh16G001060.1 vs. ExPASy TrEMBL
Match: A0A6J1J6U7 (LOW QUALITY PROTEIN: ferric reduction oxidase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483092 PE=4 SV=1)

HSP 1 Score: 1273.5 bits (3294), Expect = 0.0e+00
Identity = 631/701 (90.01%), Postives = 663/701 (94.58%), Query Frame = 0

Query: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60
           MAKEMAMAR +K +FLLTFVL LW W ML TKLYKNTWTPKL M+LNSTYFREQGTNLLL
Sbjct: 1   MAKEMAMARALKRIFLLTFVLCLWFWTMLHTKLYKNTWTPKLKMELNSTYFREQGTNLLL 60

Query: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120
           FSFP+M M+VLGCFYL LQK+SSED KPFSTQRSRGDSFLKRP+LAMNPLGI+NTLEIGF
Sbjct: 61  FSFPIMFMSVLGCFYLYLQKNSSEDSKPFSTQRSRGDSFLKRPILAMNPLGIVNTLEIGF 120

Query: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180
           AAAFVILLIWSLGNYLYVSFGNLHMH AGEK+WEAKFRSVSLRLGYIGHICWAFLFFPVT
Sbjct: 121 AAAFVILLIWSLGNYLYVSFGNLHMHGAGEKIWEAKFRSVSLRLGYIGHICWAFLFFPVT 180

Query: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240
           RGSSLLP+IGL SESTIKYHIWLGHISNLLFAL TVGFIIYWGITDQMALL EWSE YVS
Sbjct: 181 RGSSLLPLIGLASESTIKYHIWLGHISNLLFALXTVGFIIYWGITDQMALLKEWSEVYVS 240

Query: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300
           N+AGLIAM FATVIWVTSFRSIR+K F+VFFYSHH YT YTVFYLIHVGAAYVC+ILPGM
Sbjct: 241 NVAGLIAMTFATVIWVTSFRSIRQKKFKVFFYSHHLYTPYTVFYLIHVGAAYVCMILPGM 300

Query: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360
           FLFAIDRFLRFLQSQR VS+VSARHLPCG VELNLLK  ELSYSPTSVMFLNVP+ISKLQ
Sbjct: 301 FLFAIDRFLRFLQSQRHVSVVSARHLPCGVVELNLLKIPELSYSPTSVMFLNVPSISKLQ 360

Query: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420
           WHPFTITSNSKLEPDI+SV  KVGGSWTSKLHQQLSSSLDHLQV VEGPYGPAST FLRH
Sbjct: 361 WHPFTITSNSKLEPDIVSVVSKVGGSWTSKLHQQLSSSLDHLQVFVEGPYGPASTHFLRH 420

Query: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480
           ESLLLISGGSGITPFISIIREIMIVRSTKP+CHVPQVRL+CAFKNS+DLTMLDLLLPVSS
Sbjct: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPDCHVPQVRLICAFKNSMDLTMLDLLLPVSS 480

Query: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540
           TQTEISNVPLQI+AYITREEE PQTNEENL+NTIWFKPNP DSPVSEALGPN+WLLLG V
Sbjct: 481 TQTEISNVPLQIEAYITREEEPPQTNEENLVNTIWFKPNPRDSPVSEALGPNHWLLLGAV 540

Query: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600
           ISSSF+MFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFL+LACIC+ SSLVLMWQR
Sbjct: 541 ISSSFMMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLALACICVSSSLVLMWQR 600

Query: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660
           KTYVSNK+VQVL+VQSPKASPGSWI+GGERELESLPYQ LVQATNIHYG RPDLK+ILLD
Sbjct: 601 KTYVSNKRVQVLSVQSPKASPGSWIYGGERELESLPYQSLVQATNIHYGSRPDLKKILLD 660

Query: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           CKGSDIGVLVSGP  +RHDVAKICSTA GDNL FHA+SF W
Sbjct: 661 CKGSDIGVLVSGPTRLRHDVAKICSTACGDNLKFHALSFIW 701

BLAST of CmaCh16G001060.1 vs. ExPASy TrEMBL
Match: A0A1S3AZ42 (ferric reduction oxidase 4-like OS=Cucumis melo OX=3656 GN=LOC103484121 PE=4 SV=1)

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 589/704 (83.66%), Postives = 645/704 (91.62%), Query Frame = 0

Query: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60
           MAKE+AM R +KM+F LTFVLWLWLWIMLPTKLYKN W+PKLNM+LNSTYFREQGTNLLL
Sbjct: 1   MAKEIAMGRALKMIFFLTFVLWLWLWIMLPTKLYKNYWSPKLNMNLNSTYFREQGTNLLL 60

Query: 61  FSFPVMLMAVLGCFYLQLQKSSSE-DPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIG 120
           FSFP+MLMAVLGCFYL  QK SS+ DPKPFSTQR RG+SFLKRPV+ MNPLGI+NTLEI 
Sbjct: 61  FSFPIMLMAVLGCFYLHFQKESSDLDPKPFSTQRVRGESFLKRPVVVMNPLGIVNTLEIV 120

Query: 121 FAAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPV 180
           F  +F++LLIWSLGNYLYVSFG+LH    GE + E KFRSVSLR+GYIG+ICWAFLFFPV
Sbjct: 121 FGVSFLLLLIWSLGNYLYVSFGHLHRDNVGETIGELKFRSVSLRVGYIGNICWAFLFFPV 180

Query: 181 TRGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYV 240
           TRGSSLLP+IGLTSES+IKYHIWLGH+SNLLFALHT+GFIIYW +TDQMALL +WSEDYV
Sbjct: 181 TRGSSLLPLIGLTSESSIKYHIWLGHLSNLLFALHTIGFIIYWALTDQMALLKQWSEDYV 240

Query: 241 SNIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPG 300
           SN+AG+IAM FAT+IWVTSF+SIRRKMFEVF+Y+HHFY LY +FYLIHVG AYVCLILPG
Sbjct: 241 SNVAGVIAMTFATIIWVTSFQSIRRKMFEVFYYTHHFYILYIIFYLIHVGVAYVCLILPG 300

Query: 301 MFLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKL 360
           +FL+ IDR+LRFLQSQRRVSLVSARHLPCG VELN LKS ELSY+PTSVMF+NVP+ISKL
Sbjct: 301 IFLWFIDRYLRFLQSQRRVSLVSARHLPCGVVELNFLKSPELSYNPTSVMFINVPSISKL 360

Query: 361 QWHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLR 420
           QWHPFT+TSN KLEPDI+SV IK GGSWTSKLH+ LSSSLDHLQVSVEGPYGPAST FLR
Sbjct: 361 QWHPFTVTSNCKLEPDIVSVVIKSGGSWTSKLHKHLSSSLDHLQVSVEGPYGPASTHFLR 420

Query: 421 HESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVS 480
           HE LLLISGGSGITPFISIIREIMIVRSTK N HVPQ+RL+CAFKNSIDLTMLDLLLPVS
Sbjct: 421 HEYLLLISGGSGITPFISIIREIMIVRSTKVNFHVPQIRLICAFKNSIDLTMLDLLLPVS 480

Query: 481 STQTE-ISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLG 540
           STQTE ISNVPLQI+AYITRE+EQPQTN ENLINTIWFKPNP+DSP+SEALG N+WLLLG
Sbjct: 481 STQTELISNVPLQIEAYITREKEQPQTNSENLINTIWFKPNPLDSPISEALGRNSWLLLG 540

Query: 541 TVISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMW 600
            +ISSSFVMFLILLGI TRYYIYPIE+NGD VYNYTYKVLWDMFL+ ACICI SS V +W
Sbjct: 541 AIISSSFVMFLILLGITTRYYIYPIEQNGDVVYNYTYKVLWDMFLAFACICISSSFVFLW 600

Query: 601 QRKTYVSN-KQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRI 660
           QRKT  SN KQ+Q+ N QSPKASPGSWIFGG+ ELESLPYQ LVQATNIHYGGRPDL +I
Sbjct: 601 QRKTNASNDKQIQIHNFQSPKASPGSWIFGGDSELESLPYQSLVQATNIHYGGRPDLNKI 660

Query: 661 LLDCKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           L DCKGSDIGVLVSGP GMRHDVAKICS+AYGDNL FH+MSFTW
Sbjct: 661 LFDCKGSDIGVLVSGPTGMRHDVAKICSSAYGDNLKFHSMSFTW 704

BLAST of CmaCh16G001060.1 vs. NCBI nr
Match: XP_022984974.1 (ferric reduction oxidase 4-like [Cucurbita maxima])

HSP 1 Score: 1401.3 bits (3626), Expect = 0.0e+00
Identity = 701/701 (100.00%), Postives = 701/701 (100.00%), Query Frame = 0

Query: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60
           MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL
Sbjct: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60

Query: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120
           FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF
Sbjct: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120

Query: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180
           AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT
Sbjct: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180

Query: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240
           RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS
Sbjct: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240

Query: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300
           NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM
Sbjct: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300

Query: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360
           FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ
Sbjct: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360

Query: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420
           WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH
Sbjct: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420

Query: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480
           ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS
Sbjct: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480

Query: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540
           TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV
Sbjct: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540

Query: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600
           ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR
Sbjct: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600

Query: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660
           KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD
Sbjct: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660

Query: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW
Sbjct: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 701

BLAST of CmaCh16G001060.1 vs. NCBI nr
Match: XP_022922912.1 (ferric reduction oxidase 4-like [Cucurbita moschata])

HSP 1 Score: 1382.1 bits (3576), Expect = 0.0e+00
Identity = 689/701 (98.29%), Postives = 694/701 (99.00%), Query Frame = 0

Query: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60
           MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL
Sbjct: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60

Query: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120
           FSFPVMLMAVLGCFYL LQKSSSEDPKPFSTQRSRGDSFLKRPVL MNPLGILNTLEIGF
Sbjct: 61  FSFPVMLMAVLGCFYLHLQKSSSEDPKPFSTQRSRGDSFLKRPVLVMNPLGILNTLEIGF 120

Query: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180
           AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT
Sbjct: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180

Query: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240
           RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS
Sbjct: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240

Query: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300
           NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCL+LPGM
Sbjct: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLVLPGM 300

Query: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360
           FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKS ELSYSPTSVMFLNVPTISKLQ
Sbjct: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSPELSYSPTSVMFLNVPTISKLQ 360

Query: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420
           WHPFT+TSNSKLEP IISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPAST FLRH
Sbjct: 361 WHPFTVTSNSKLEPAIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRH 420

Query: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480
           ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS
Sbjct: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480

Query: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540
           TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNP+DSPV+EALGPNNWLLLG V
Sbjct: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPLDSPVTEALGPNNWLLLGAV 540

Query: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600
           ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR
Sbjct: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600

Query: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660
           KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLP+QPLVQATNIHYGGRPDLKRILLD
Sbjct: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPFQPLVQATNIHYGGRPDLKRILLD 660

Query: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           CKGSDIGVLVSGP GMRHDVAKICSTAYGDNLNFHAMSFTW
Sbjct: 661 CKGSDIGVLVSGPTGMRHDVAKICSTAYGDNLNFHAMSFTW 701

BLAST of CmaCh16G001060.1 vs. NCBI nr
Match: KAG6576705.1 (Ferric reduction oxidase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1382.1 bits (3576), Expect = 0.0e+00
Identity = 689/701 (98.29%), Postives = 694/701 (99.00%), Query Frame = 0

Query: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60
           MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL
Sbjct: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60

Query: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120
           FSFPVMLMAVLGCFYL LQKSSSEDPKPFSTQRSRGDSFLKRPVL MNPLGILNTLEIGF
Sbjct: 61  FSFPVMLMAVLGCFYLHLQKSSSEDPKPFSTQRSRGDSFLKRPVLVMNPLGILNTLEIGF 120

Query: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180
           AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT
Sbjct: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180

Query: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240
           RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS
Sbjct: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240

Query: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300
           NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCL+LPGM
Sbjct: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLVLPGM 300

Query: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360
           FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKS ELSYSPTSVMFLNV TISKLQ
Sbjct: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSPELSYSPTSVMFLNVTTISKLQ 360

Query: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420
           WHPFT+TSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPAST FLRH
Sbjct: 361 WHPFTVTSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRH 420

Query: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480
           ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS
Sbjct: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480

Query: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540
           TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNP+DSPV+EALGPNNWLLLG V
Sbjct: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPLDSPVTEALGPNNWLLLGAV 540

Query: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600
           ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR
Sbjct: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600

Query: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660
           KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD
Sbjct: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660

Query: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           CKGSDIGVLVSGP GMRHDVAKICSTA+GDNLNFHAMSFTW
Sbjct: 661 CKGSDIGVLVSGPTGMRHDVAKICSTAFGDNLNFHAMSFTW 701

BLAST of CmaCh16G001060.1 vs. NCBI nr
Match: XP_023552403.1 (ferric reduction oxidase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1377.1 bits (3563), Expect = 0.0e+00
Identity = 684/701 (97.57%), Postives = 692/701 (98.72%), Query Frame = 0

Query: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60
           MAKEMAM RGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL
Sbjct: 1   MAKEMAMVRGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60

Query: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120
           FSFPVMLMAVLGCFYL LQKSSSEDPKPFSTQRSRGDSFLKRP+L MNPLGILNTLEIGF
Sbjct: 61  FSFPVMLMAVLGCFYLHLQKSSSEDPKPFSTQRSRGDSFLKRPILVMNPLGILNTLEIGF 120

Query: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180
           AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT
Sbjct: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180

Query: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240
           RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS
Sbjct: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240

Query: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300
           NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCL+LPGM
Sbjct: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLVLPGM 300

Query: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360
           FLFAIDRF+RFLQSQRRVSLVSARHLPCGAVELNLLKS ELSYSPTSVMFLNVP+ISKLQ
Sbjct: 301 FLFAIDRFIRFLQSQRRVSLVSARHLPCGAVELNLLKSPELSYSPTSVMFLNVPSISKLQ 360

Query: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420
           WHPFT+TSNSKLEPDIISV  KVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPAST FLRH
Sbjct: 361 WHPFTVTSNSKLEPDIISVVSKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRH 420

Query: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480
           ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS
Sbjct: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480

Query: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540
           TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNP+DSPV+EALGPNNWLLLG V
Sbjct: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPLDSPVTEALGPNNWLLLGAV 540

Query: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600
           ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR
Sbjct: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600

Query: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660
           KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDL+RILLD
Sbjct: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLERILLD 660

Query: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           CKGSDIGVLVSGP GMRHDVAKICSTAYGDNLNFHAMSFTW
Sbjct: 661 CKGSDIGVLVSGPTGMRHDVAKICSTAYGDNLNFHAMSFTW 701

BLAST of CmaCh16G001060.1 vs. NCBI nr
Match: XP_022922911.1 (ferric reduction oxidase 4-like [Cucurbita moschata])

HSP 1 Score: 1310.0 bits (3389), Expect = 0.0e+00
Identity = 645/701 (92.01%), Postives = 676/701 (96.43%), Query Frame = 0

Query: 1   MAKEMAMARGVKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLL 60
           MAKEMAMAR +KM+FLLTFVLWLWLWIMLPTKLYKNTWTPKL M+LNSTYFREQGTNLLL
Sbjct: 1   MAKEMAMARALKMIFLLTFVLWLWLWIMLPTKLYKNTWTPKLKMELNSTYFREQGTNLLL 60

Query: 61  FSFPVMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGF 120
           FSFP+M MAVLGCFYL LQK+SSED KPFSTQRSRGDSFLKRP+LAMNPLGI+NTLEIGF
Sbjct: 61  FSFPIMFMAVLGCFYLHLQKNSSEDSKPFSTQRSRGDSFLKRPILAMNPLGIVNTLEIGF 120

Query: 121 AAAFVILLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 180
           AAAFVILLIWSLGNYLYVSFGNLHMH AGEK+WEAKFRSVSLRLGYIGHICWAFLFFPVT
Sbjct: 121 AAAFVILLIWSLGNYLYVSFGNLHMHGAGEKIWEAKFRSVSLRLGYIGHICWAFLFFPVT 180

Query: 181 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 240
           RGSSLLP+IGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALL EWSE YVS
Sbjct: 181 RGSSLLPLIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLKEWSEVYVS 240

Query: 241 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 300
           N+AGLIAMAFATVIWVTSFRSIR+KMFEVFFYSHH YTLYTVFYLIHVGAAYVC+ILPGM
Sbjct: 241 NVAGLIAMAFATVIWVTSFRSIRQKMFEVFFYSHHLYTLYTVFYLIHVGAAYVCMILPGM 300

Query: 301 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 360
           FLFAIDRFLRFLQSQR VSLVSARHLPCG VELNLLKS ELSYSPTSVMFLNVP+ISKLQ
Sbjct: 301 FLFAIDRFLRFLQSQRHVSLVSARHLPCGVVELNLLKSPELSYSPTSVMFLNVPSISKLQ 360

Query: 361 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRH 420
           WHPFTITSNSKLEPDI+SV  KVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPAST FLRH
Sbjct: 361 WHPFTITSNSKLEPDIVSVVSKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTHFLRH 420

Query: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 480
           ESLLLISGGSGITPFISIIREIMIVRSTKP+CHVPQVRL+CAFKNS+DLTMLDLLLPVSS
Sbjct: 421 ESLLLISGGSGITPFISIIREIMIVRSTKPDCHVPQVRLICAFKNSMDLTMLDLLLPVSS 480

Query: 481 TQTEISNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTV 540
           TQTEISNVPLQI+AYITREEE PQTNEENL+NTIWFKPNP+DSPVSEALGPN+WLLLG V
Sbjct: 481 TQTEISNVPLQIEAYITREEEPPQTNEENLVNTIWFKPNPLDSPVSEALGPNHWLLLGAV 540

Query: 541 ISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQR 600
           ISSSF+MFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFL+LACIC+ SS VLMWQR
Sbjct: 541 ISSSFMMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLALACICVSSSFVLMWQR 600

Query: 601 KTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 660
           KTYVSNK+VQVL+VQSPKASPGSWI+GGERELESLPYQ LVQATN+HYG RPDLK+ILLD
Sbjct: 601 KTYVSNKRVQVLSVQSPKASPGSWIYGGERELESLPYQSLVQATNVHYGSRPDLKKILLD 660

Query: 661 CKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           CKGSDIGVLVSGP  +RHDVAKICSTA GDNL FHA+SFTW
Sbjct: 661 CKGSDIGVLVSGPTRLRHDVAKICSTACGDNLKFHALSFTW 701

BLAST of CmaCh16G001060.1 vs. TAIR 10
Match: AT5G23980.1 (ferric reduction oxidase 4 )

HSP 1 Score: 775.8 bits (2002), Expect = 3.0e-224
Identity = 381/699 (54.51%), Postives = 517/699 (73.96%), Query Frame = 0

Query: 11  VKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDLNSTYFREQGTNLLLFSFPVMLMAV 70
           VK + ++ F+ W+ +WIM+ T L+K+ WTPKL+  LN+TYF  QGTNL+L + P+M +AV
Sbjct: 8   VKTLMVVLFLGWILVWIMISTNLFKSKWTPKLSKYLNTTYFGPQGTNLVLLTVPMMFIAV 67

Query: 71  LGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGFAAAFVILLIW 130
           L C YL +QK  ++  + +  +R  G     R ++ MNPLGI+   E+ F+  FV LL W
Sbjct: 68  LSCVYLHIQKKPTQPQREWKLKRIMG-----RVIMVMNPLGIVTATELTFSLLFVALLAW 127

Query: 131 SLGNYLYVSFGNLHMH-VAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSSLLPII 190
           SL NYLY+S+ ++H+H     K+W+AKFR+  LR+GY+G+ICWAFLFFPVTR S++LP++
Sbjct: 128 SLYNYLYLSY-HVHLHNDDNAKIWQAKFRAFGLRIGYVGNICWAFLFFPVTRASTILPLV 187

Query: 191 GLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAGLIAMA 250
           GLTSES+IKYHIWLGH+SN  F +HTV F+IYW + +++     W+  YV N+AG IAM 
Sbjct: 188 GLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMINKLMETFAWNPTYVPNLAGTIAMV 247

Query: 251 FATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGMFLFAIDRFL 310
               +WVTS  S RRK FE+FFY+HH Y LY VFY+IHVG ++ C+ILP +FLF IDR+L
Sbjct: 248 IGIAMWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYVIHVGDSWFCMILPNIFLFFIDRYL 307

Query: 311 RFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQWHPFTITSN 370
           RFLQS +R  LVSAR LP   +EL   K+  L Y+PTS++FL+VP+ISK+QWHPFTITS+
Sbjct: 308 RFLQSTKRSRLVSARILPSDNLELTFSKTPGLHYTPTSILFLHVPSISKIQWHPFTITSS 367

Query: 371 SKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRHESLLLISGG 430
           S LE D +SV I+  GSWT KL+  LSSS+D L+VS EGPYGP S    RH SL+L+SGG
Sbjct: 368 SNLEKDTLSVVIRRQGSWTQKLYTHLSSSIDSLEVSTEGPYGPNSFDVSRHNSLILVSGG 427

Query: 431 SGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQTEISNVP 490
           SGITPFIS+IRE +I +S   +  +P V LVC+FK+  DL  LDL+ P+  + ++IS + 
Sbjct: 428 SGITPFISVIRE-LISQSQNKSTKLPDVLLVCSFKHYHDLAFLDLIFPLDMSASDISRLN 487

Query: 491 LQIDAYITREEEQPQTNEEN-LINTIWFKPNPMDSPVSEALGPNNWLLLGTVISSSFVMF 550
           L+I+AYITRE+++P+T +++ L+ T WFKP P+DSP+S  LGPNN+L LG VI SSFVMF
Sbjct: 488 LRIEAYITREDKKPETTDDHRLLQTKWFKPQPLDSPISPVLGPNNFLWLGVVILSSFVMF 547

Query: 551 LILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRKTYVS--- 610
           L+L+GI+TRYYIYP++ N  ++YN++Y+ LWDMFL  ACI I SS+V +W++K       
Sbjct: 548 LLLIGIVTRYYIYPVDHNTGSIYNFSYRGLWDMFLGSACIFISSSVVFLWRKKQNKEGDK 607

Query: 611 --NKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLDCKG 670
               QVQ +  Q+P +SPGSW  G ERELES+PYQ +VQAT++H+G +P+LK+ILL+ +G
Sbjct: 608 EFKNQVQSVEFQTPTSSPGSWFHGHERELESVPYQSIVQATSVHFGSKPNLKKILLEAEG 667

Query: 671 S-DIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           S D+GV+V GP  MRH+VAKICS+    NL+F A+SF W
Sbjct: 668 SEDVGVMVCGPRKMRHEVAKICSSGLAKNLHFEAISFNW 699

BLAST of CmaCh16G001060.1 vs. TAIR 10
Match: AT5G23990.1 (ferric reduction oxidase 5 )

HSP 1 Score: 676.0 bits (1743), Expect = 3.2e-194
Identity = 347/702 (49.43%), Postives = 472/702 (67.24%), Query Frame = 0

Query: 11  VKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKL----NMDLNSTYFREQGTNLLLFSFPVM 70
           VKM+ ++ F+ W+++WIM+ T  ++N WTPKL    +   + +     G+ L LF++P  
Sbjct: 8   VKMLMVVLFLGWIFVWIMISTNRFQNIWTPKLAKHESCATHGSNDVHCGSELCLFAYP-- 67

Query: 71  LMAVLGCFYLQLQKSSSEDPKPFSTQRSRGDSFLKRPVLAMNPLGILNTLEIGFAAAFVI 130
                        K++  + K    +R  G+           P+G  ++  + F +    
Sbjct: 68  -------------KATLPNSKRMEGKRENGEG-----DDGYEPIGDSDSHRVDFFSV--- 127

Query: 131 LLIWSLGNYLYVSFGNLHMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSSLL 190
                                       AKFR+  LR+GY+GH CWAFLFFPVTR S++L
Sbjct: 128 ----------------------------AKFRAFGLRIGYVGHYCWAFLFFPVTRASTIL 187

Query: 191 PIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAGLI 250
           P++GLTSES+IKYHIWLGH+SN  F +HTV F+IYW + +++     W+  YV N+AG I
Sbjct: 188 PLVGLTSESSIKYHIWLGHVSNFCFLVHTVVFLIYWAMVNKLMETFAWNATYVPNLAGTI 247

Query: 251 AMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGMFLFAID 310
           AM     IWVTS  S RRK FE+FFY+HH Y LY VFY IHVG ++ C+ILP +FLF ID
Sbjct: 248 AMVIGIAIWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYAIHVGDSWFCMILPNIFLFFID 307

Query: 311 RFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQWHPFTI 370
           R+LRFLQS +R  LVSA+ LP   +EL   K+S L Y+PTS++FL+VP+ISKLQWHPFTI
Sbjct: 308 RYLRFLQSTKRSRLVSAKILPSDNLELTFAKTSGLHYTPTSILFLHVPSISKLQWHPFTI 367

Query: 371 TSNSKLEPDIISVAIKVGGSWTSKLHQQLSSSLDHLQVSVEGPYGPASTRFLRHESLLLI 430
           TS+S LE D +SV I+  GSWT KL+  LSSS+D L+VS EGPYGP S    RH+SL+L+
Sbjct: 368 TSSSNLEKDTLSVVIRKQGSWTQKLYTHLSSSIDSLEVSTEGPYGPNSFDVSRHDSLILV 427

Query: 431 SGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQTEIS 490
            GGSG+TPFIS+IRE +I +S   +  +P V LVCAFKN  DL  LDL+ P   + ++IS
Sbjct: 428 GGGSGVTPFISVIRE-LIFQSQNRSTKLPNVLLVCAFKNYHDLAFLDLIFPSDISVSDIS 487

Query: 491 NVPLQIDAYITREEEQPQTNEEN-LINTIWFKPNPMDSPVSEALGPNNWLLLGTVISSSF 550
            + L+I+AYITRE+++P+T +++ L+ T WFKP P+DSP+S  LGPNN+L LG VI SSF
Sbjct: 488 KLNLRIEAYITREDKKPETTDDHKLLQTKWFKPQPLDSPISPVLGPNNFLWLGVVILSSF 547

Query: 551 VMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRKTYV- 610
           VMFL+L+GI+TRYYIYP++ N  ++YN+TY+VLW MFL   CI I SS++ +W++K    
Sbjct: 548 VMFLLLIGIVTRYYIYPVDHNTGSIYNFTYRVLWVMFLGCVCIFISSSIIFLWRKKENKE 607

Query: 611 ----SNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLD 670
               S KQVQ +  Q+P +SPGSW  G ERELES+PYQ +VQAT++H+G +P+LK+IL +
Sbjct: 608 GDKDSKKQVQSVEFQTPTSSPGSWFHGHERELESVPYQSIVQATSVHFGSKPNLKKILFE 657

Query: 671 CKGS-DIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
            +GS D+GV+V GP  MRH+VAKICS+    NL+F A+SF W
Sbjct: 668 AEGSEDVGVMVCGPKKMRHEVAKICSSGLAKNLHFEAISFNW 657

BLAST of CmaCh16G001060.1 vs. TAIR 10
Match: AT1G01580.1 (ferric reduction oxidase 2 )

HSP 1 Score: 675.6 bits (1742), Expect = 4.2e-194
Identity = 346/708 (48.87%), Postives = 497/708 (70.20%), Query Frame = 0

Query: 7   MARGV-KMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDL-NSTYFREQGTNLLLFSFP 66
           M +GV K + ++ F+  + LWIM+PT  Y+  W P L +    STYF   GT L ++ FP
Sbjct: 22  MIKGVTKFLMMVIFLGTIMLWIMMPTLTYRTKWLPHLRIKFGTSTYFGATGTTLFMYMFP 81

Query: 67  VMLMAVLGCFYLQLQKSSSEDPKPFSTQRSRGD-SFLKRPVLAMNPLGILNTLEIGFAAA 126
           +M++A LGC YL  +   S  P     +   G  S L++P+L   PLGI++  EI F A 
Sbjct: 82  MMVVACLGCVYLHFKNRKS--PHHIDRETKGGVWSKLRKPMLVKGPLGIVSVTEITFLAM 141

Query: 127 FVILLIWSLGNYLYVSFGNLHMHVAG---EKVWEAKFRSVSLRLGYIGHICWAFLFFPVT 186
           FV LL+W    YL  SF  +    A    E +W+AK  S +LRLG IG+IC AFLF PV 
Sbjct: 142 FVALLLWCFITYLRNSFATITPKSAAAHDESLWQAKLESAALRLGLIGNICLAFLFLPVA 201

Query: 187 RGSSLLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVS 246
           RGSSLLP +GLTSES+IKYHIWLGH+   LF +H + +IIYW    +++ +I W    VS
Sbjct: 202 RGSSLLPAMGLTSESSIKYHIWLGHMVMALFTVHGLCYIIYWASMHEISQMIMWDTKGVS 261

Query: 247 NIAGLIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGM 306
           N+AG IA+A   V+W T++  IRR+ FEVFFY+H+ Y ++ +F+++HVG ++  + LPG 
Sbjct: 262 NLAGEIALAAGLVMWATTYPKIRRRFFEVFFYTHYLYIVFMLFFVLHVGISFSFIALPGF 321

Query: 307 FLFAIDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQ 366
           ++F +DRFLRFLQS+  V L++AR LP   +EL   K+S+L YSPTS+MF+N+P+ISKLQ
Sbjct: 322 YIFLVDRFLRFLQSRENVRLLAARILPSDTMELTFSKNSKLVYSPTSIMFVNIPSISKLQ 381

Query: 367 WHPFTITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSS--LDHLQVSVEGPYGPASTRFL 426
           WHPFTITS+SKLEP+ +S+ IK  G W++KLHQ+LSSS  +D L VSVEGPYGPAS  FL
Sbjct: 382 WHPFTITSSSKLEPEKLSIVIKKEGKWSTKLHQRLSSSDQIDRLAVSVEGPYGPASADFL 441

Query: 427 RHESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPV 486
           RHE+L+++ GGSGITPFIS+IR+ +I  S K  C +P++ L+CAFK S +++MLDL+LP+
Sbjct: 442 RHEALVMVCGGSGITPFISVIRD-LIATSQKETCKIPKITLICAFKKSSEISMLDLVLPL 501

Query: 487 SSTQTEI-SNVPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLL 546
           S  +TE+ S++ ++I+A+ITR+ +     +   I T+WFKP+  D  +S  LGPN+WL L
Sbjct: 502 SGLETELSSDINIKIEAFITRDNDAGDEAKAGKIKTLWFKPSLSDQSISSILGPNSWLWL 561

Query: 547 GTVISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYK-VLWDMFLSLACICICSSLVL 606
           G +++SSF++F+I++GIITRYYIYPI+ N + +Y+ T K +++ + +S++ +  CS+  +
Sbjct: 562 GAILASSFLIFMIIIGIITRYYIYPIDHNTNKIYSLTSKTIIYILVISVSIMATCSA-AM 621

Query: 607 MWQRKTY--VSNKQVQVLNVQSPKASP-GSWIFGGERELESLPYQPLVQATNIHYGGRPD 666
           +W +K Y  V +KQVQ ++  SP +SP  SW +   RE+ES P + LVQ TN+H+G RP+
Sbjct: 622 LWNKKKYGKVESKQVQNVDRPSPTSSPTSSWGYNSLREIESTPQESLVQRTNLHFGERPN 681

Query: 667 LKRILLDCKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           LK++LLD +GS +GVLV GP  MR  VA+ICS+   +NL+F ++SF+W
Sbjct: 682 LKKLLLDVEGSSVGVLVCGPKKMRQKVAEICSSGLAENLHFESISFSW 725

BLAST of CmaCh16G001060.1 vs. TAIR 10
Match: AT1G01590.1 (ferric reduction oxidase 1 )

HSP 1 Score: 634.4 bits (1635), Expect = 1.1e-181
Identity = 327/705 (46.38%), Postives = 476/705 (67.52%), Query Frame = 0

Query: 11  VKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDL-NSTYFREQGTNLLLFSFPVMLMA 70
           +K + ++  +  + +WIM+PT  YK  W   +   L  S Y+   G NLL++ FP++L+A
Sbjct: 15  IKFLMMVILMGTIVIWIMMPTSTYKEIWLTSMRAKLGKSIYYGRPGVNLLVYMFPMILLA 74

Query: 71  VLGCFYLQLQKSSSEDPKPFSTQRSRGD-SFLKRPVLAMNPLGILNTLEIGFAAAFVILL 130
            LGC YL L+KS++ +      ++ R     L+RP+L   PLGI+   E+ F   F+ LL
Sbjct: 75  FLGCIYLHLKKSTTVNQFNSGVEKKRAKFGALRRPMLVNGPLGIVTVTEVMFLTMFMALL 134

Query: 131 IWSLGNYLYVSFGNLHMHVA---GEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSSL 190
           +WSL NY+Y +F N+    A   G  +W+A+   +++R+G +G+IC AFLF+PV RGSSL
Sbjct: 135 LWSLANYMYRTFVNVTSESAATDGNNLWQARLDLIAVRIGIVGNICLAFLFYPVARGSSL 194

Query: 191 LPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQM-ALLIEWSEDYVSNIAG 250
           L  +GLTSES+IKYHIWLGH+  ++F  H + + IYW   +Q+ + ++EW    VSN+AG
Sbjct: 195 LAAVGLTSESSIKYHIWLGHLVMIIFTSHGLCYFIYWISKNQLVSKMLEWDRTAVSNLAG 254

Query: 251 LIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGMFLFA 310
            IA+    ++WVT++  IRR++FEVFFYSH+ Y ++ +F++ HVG ++  + LPG ++F 
Sbjct: 255 EIALVAGLMMWVTTYPKIRRRLFEVFFYSHYLYIVFMLFFVFHVGISHALIPLPGFYIFL 314

Query: 311 IDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQWHPF 370
           +DRFLRFLQS+  V LVSAR LPC  VELN  K+  L YSPTS MF+N+P+ISKLQWHPF
Sbjct: 315 VDRFLRFLQSRNNVKLVSARVLPCDTVELNFSKNPMLMYSPTSTMFVNIPSISKLQWHPF 374

Query: 371 TITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSS----LDHLQVSVEGPYGPASTRFLRH 430
           TI S+SKLEP+ +SV IK  G W++KL+  LSSS    ++ L VSVEGPYGP+ST FLRH
Sbjct: 375 TIISSSKLEPETLSVMIKSQGKWSTKLYDMLSSSSSDQINRLAVSVEGPYGPSSTDFLRH 434

Query: 431 ESLLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSS 490
           ESL+++SGGSGITPFISI+R++  + ST   C +P++ L+CAFKNS DL+MLDL+LP S 
Sbjct: 435 ESLVMVSGGSGITPFISIVRDLFYMSSTH-KCKIPKMTLICAFKNSSDLSMLDLILPTSG 494

Query: 491 TQTEISN-VPLQIDAYITREEE---QPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLL 550
             T++++ V +QI A++TREE+   +  T+  N+I T  FKPN  D P+S  LGPN+WL 
Sbjct: 495 LTTDMASFVDIQIKAFVTREEKTSVKESTHNRNIIKTRHFKPNVSDQPISPILGPNSWLC 554

Query: 551 LGTVISSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVL 610
           L  ++SSSF++F++++ IITRY+I+PI++N +  Y + YK L  +      +   S+  +
Sbjct: 555 LAAILSSSFMIFIVIIAIITRYHIHPIDQNSEK-YTWAYKSLIYLVSISITVVTTSTAAM 614

Query: 611 MWQRKTYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKR 670
           +W +K Y +     V N+ SP              +ES P Q + Q+T+IHYG RP+L +
Sbjct: 615 LWNKKKYYAKNDQYVDNL-SPVI------------IESSPQQLISQSTDIHYGERPNLNK 674

Query: 671 ILLDCKGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           +L+  KGS +G+LV GP  MR  VAKICS    +NL+F ++SF+W
Sbjct: 675 LLVGLKGSSVGILVCGPKKMRQKVAKICSFGSAENLHFESISFSW 704

BLAST of CmaCh16G001060.1 vs. TAIR 10
Match: AT1G23020.2 (ferric reduction oxidase 3 )

HSP 1 Score: 606.7 bits (1563), Expect = 2.4e-173
Identity = 324/700 (46.29%), Postives = 468/700 (66.86%), Query Frame = 0

Query: 11  VKMVFLLTFVLWLWLWIMLPTKLYKNTWTPKLNMDL-NSTYFREQGTNLLLFSFPVMLMA 70
           +K++ ++  +  + +WIM+PT  YK  W   +   L  S YF + G NLL++ FP++L+A
Sbjct: 38  IKLLTMVILMGTVVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFPMILLA 97

Query: 71  VLGCFYLQLQKSSSEDPKPFSTQRSRGDSF--LKRPVLAMNPLGILNTLEIGFAAAFVIL 130
            LG  YL L+K +  +       R + D F  LKRP+L    LGI+   E+ F   F+ L
Sbjct: 98  SLGSIYLHLKKQTRVNQFNSRMDRKKIDKFGALKRPMLVKAGLGIVTVTEVMFLMMFMAL 157

Query: 131 LIWSLGNYLYVSFGNL---HMHVAGEKVWEAKFRSVSLRLGYIGHICWAFLFFPVTRGSS 190
           L+WSL NY Y +F  +    +   G+ +W+A+  S+++RLG  G+IC  FLF+PV RGSS
Sbjct: 158 LLWSLANYFYHTFVTITPQSLPTDGDNLWQARLDSIAVRLGLTGNICLGFLFYPVARGSS 217

Query: 191 LLPIIGLTSESTIKYHIWLGHISNLLFALHTVGFIIYWGITDQMALLIEWSEDYVSNIAG 250
           LL  +GLTSES+ KYHIWLG++   LF  H + + IYW  T+Q++ ++EW    +S++AG
Sbjct: 218 LLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQVSQMLEWDRTGISHLAG 277

Query: 251 LIAMAFATVIWVTSFRSIRRKMFEVFFYSHHFYTLYTVFYLIHVGAAYVCLILPGMFLFA 310
            IA+    ++W T+F +IRR+ FEVFFY+H+ Y ++ +F++ HVG +Y  +  PG ++F 
Sbjct: 278 EIALVAGLLMWATTFPAIRRRFFEVFFYTHYLYMVFMLFFVFHVGISYALISFPGFYIFM 337

Query: 311 IDRFLRFLQSQRRVSLVSARHLPCGAVELNLLKSSELSYSPTSVMFLNVPTISKLQWHPF 370
           +DRFLRFLQS+  V LVSAR LPC  VELN  K+  L YSPTS++F+N+P+ISKLQWHPF
Sbjct: 338 VDRFLRFLQSRNNVKLVSARVLPCETVELNFSKNPMLMYSPTSILFVNIPSISKLQWHPF 397

Query: 371 TITSNSKLEPDIISVAIKVGGSWTSKLHQQLSSS--LDHLQVSVEGPYGPASTRFLRHES 430
           TITS+SKLEP  +SV IK  G W+SKLH  L+SS  +DHL VSVEGPYGPAST +LRH+S
Sbjct: 398 TITSSSKLEPKKLSVMIKSQGKWSSKLHHMLASSNQIDHLAVSVEGPYGPASTDYLRHDS 457

Query: 431 LLLISGGSGITPFISIIREIMIVRSTKPNCHVPQVRLVCAFKNSIDLTMLDLLLPVSSTQ 490
           L+++SGGSGITPFISIIR+++ V ST      P++ L+CAFKNS DL+ML+L+LP S   
Sbjct: 458 LVMVSGGSGITPFISIIRDLLYVSSTNA-YKTPKITLICAFKNSSDLSMLNLILPNS--- 517

Query: 491 TEISN-VPLQIDAYITREEEQPQTNEENLINTIWFKPNPMDSPVSEALGPNNWLLLGTVI 550
           TEIS+ + +QI A++TR  E+  T   N+I T+ FKP   D P+S  LGPN+WL L T++
Sbjct: 518 TEISSFIDIQIKAFVTR--EKVSTCNMNIIKTLSFKPYVSDQPISPILGPNSWLWLATIL 577

Query: 551 SSSFVMFLILLGIITRYYIYPIERNGDAVYNYTYKVLWDMFLSLACICICSSLVLMWQRK 610
           SSSF++F+I++ II+RY+IYPI+++     +    +++ + +S++ +   S++ ++  +K
Sbjct: 578 SSSFMIFIIIIAIISRYHIYPIDQSSKEYTSAYTSLIYLLAISISVVA-TSTVAMLCNKK 637

Query: 611 TYVSNKQVQVLNVQSPKASPGSWIFGGERELESLPYQPLVQATNIHYGGRPDLKRILLDC 670
           +Y      Q ++  SP              +ES P Q L + TNIHYG RP+L ++L+  
Sbjct: 638 SYFKG-LYQNVDALSPLM------------IESSPDQLLPEFTNIHYGERPNLNKLLVGL 697

Query: 671 KGSDIGVLVSGPMGMRHDVAKICSTAYGDNLNFHAMSFTW 702
           KGS +GVLV GP  MR +VAKICS     NL F ++SF W
Sbjct: 698 KGSSVGVLVCGPRKMREEVAKICSFGSAANLQFESISFNW 717