CmaCh15G012470.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh15G012470.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Beta-carotene 3-hydroxylase, chloroplastic
Location	Cma_Chr15: 7922649 .. 7924937 (-)
Sequence length	1075
RNA-Seq Expression	CmaCh15G012470.1
Synteny	CmaCh15G012470.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAACTCAAATCCCTTTATAAACGCTTTCTTTTCCTTTCCAATTTCTTCTCGACTCGATCCTCAATCCGATCCGAAGCTCGCCGGCGTGCGTTCATGGCGACGGGGATCTCGGCCGTTGCAACCGCGGCGCCGTACCGGAGAATAATTCAACGACCGGCGAAAGCTCCAGCTCCGGCGAACCGTGTTCCATGGCGGTGCTTTGCAAAACAGAGCACGAGAAGATTGTCGACAGTGAAGAAGAAATGTGGGGTGAGTGAAGTTGAAATGGTTAGGGATTTGAAGGTAATCGTGGAGGAGAAAATGGGGAGGAAGAAAGCGGAAAGGGATGCTTATCTTGTTGCTGCAATTGTGTCGAGCTTCGGGATCACGTCCATGGCTGCCATCGCTGTCTATTACAGATTCTCCTGGCAACTCGAGGTATTTTTTCCCCTCCCCTTTTTTCTTTTACCAATTTTCTTAATACTCTAATTGATATAATCGAATTGGGATATAAGGTGTATACTCATGATTGATATAATCGAATTGGGATCTAAGGTGTATACTATGATTGATATAATCGAATTGGGATCTAAGGTGTATACTCTGATTGATATAATCGAAATGGAATTATAATCGAATTAGGATCTAAGGTGCATACTCTGATTGATATAAGATCTAAGGTGTATAATTCTGATTGAATTGAATTGAGATCTAAAGTATATTTTGATTATATTAATAATAGTCTGATTATATAATTAATTATTATAATATATTTTCTAAATATTATATCTCAATATTTTCCCATTTTTTATTTAAATTTAACTTTGTTAATAAATTTTAATTCATAACAAAATTATGGAAAATAACTCGAATTACATTTCATTTACAGGGTGGAGATTTTCCGGTTTTGGAAATGTTTGGTACTTTCGCACTATCGGTTGGAGCTGCTGTAAGTTGAAGCCTATAAAATTATGGTTGCATTAATAAAAATATTTAATTTTATTTTAAAAGTTTCATTAATATTTCAAAAAAAAAAATTAAAAAATATCATTTGTTATTATAGTTAAAAAAAAAATATTCATAAAATTTTAAAGATAGTTAAAAAATTTAGAAATTATAGTAGTAATTTTTTTTATTTTTAAAAGGGTACTTTTCAAGTTCAAATAATAAGTTTAAGAGTATTATTAAAATTTTTGAAAATTTAATAGTATTTTTGAGGTAAATTATCAAATTTAAAAATGATCTAAAATAATTTTTTTTAGGTGGGGATGGAATTCTGGGCGAGATGGGCACATAAACAACTGTGGCATGATGCGCTATGGAATATGCACAAGGTAATAGCTCGTTACATTTAAATGTGTGAACTTTCGCTATGAAATATGCACAAGGTAATAGCGTGATTATTTAAAAAAATATTTTGCGTTAAAATTTTGTAAATAATTCAAACTAAACATTTTGAAATTACATGAATGGGTGAGGCTAGTCTCATCATAGGGCGAGGGATGGAGCGTTTGAATTGAACGATGTGTTCGCAATCATCAACGCGGTGCCTGCCATTGCTCTTCTTTCTTTCGGCTTCTTCAATCAGGGCTTCTTCCCCGGTCTATGTTTTGGTGCTGTATGTCTCCTTCTCCCTTTACTAAAAACAACATTAATTTCTACGAAATTATTTGAAATTTTGTAAATATTAAAACAATTAATTTAGTAAAGTGGTGAAGGCTGTCGAACTCCAGGTCAGATTTTCATGACGTGTCATGTTGTGATTCGGCAGGGTTTGGGAATTACGGTGTTTGGGATGGCCTACATGTTCGTCCATGATGGCCTCGTGCACCGTCGTTTCCCTGTGGGTCCCATAGCTGCTGTCCCCTACTTACGACGTGTCGCTGCTGCCCATCATGTAAGTCATTCCCGACATGTCGTTTGTTTTTTGGGAGTTAAATTTTAATTTTTTATTTTTTTGGTGCAGATCCATCATACGGACAAATTCGATGGCGTCCCGTACGGGTTGTTTTTAGGACCCAAGGTTAGTTCCGTCATTTTACTCGCTAATTATTATTTTTTTGTTACTTTGAATGAAAACGACGCCGTTTGGATTGCTGCAGGAACTGGAAGACGTGGATGGCGAGGAAGAGCTGCAGAATGAAATTAAAAAGAGAAGCAAAGTTTACAGAAATTAAATTTAATTTTCTCATTATATTAGTAATAATCAACAATAGGTGGTTATTATTTTTTTAATTTTTTTCTCTTCGTGTAATTAATTATTTTTTATTAACACGAATACTCGGTTAAGCAATAAATTTAATTCATA

mRNA sequence

AAGAACTCAAATCCCTTTATAAACGCTTTCTTTTCCTTTCCAATTTCTTCTCGACTCGATCCTCAATCCGATCCGAAGCTCGCCGGCGTGCGTTCATGGCGACGGGGATCTCGGCCGTTGCAACCGCGGCGCCGTACCGGAGAATAATTCAACGACCGGCGAAAGCTCCAGCTCCGGCGAACCGTGTTCCATGGCGGTGCTTTGCAAAACAGAGCACGAGAAGATTGTCGACAGTGAAGAAGAAATGTGGGGTGAGTGAAGTTGAAATGGTTAGGGATTTGAAGGTAATCGTGGAGGAGAAAATGGGGAGGAAGAAAGCGGAAAGGGATGCTTATCTTGTTGCTGCAATTGTGTCGAGCTTCGGGATCACGTCCATGGCTGCCATCGCTGTCTATTACAGATTCTCCTGGCAACTCGAGGGTGGAGATTTTCCGGTTTTGGAAATGTTTGGTACTTTCGCACTATCGGTTGGAGCTGCTGTGGGGATGGAATTCTGGGCGAGATGGGCACATAAACAACTGTGGCATGATGCGCTATGGAATATGCACAAGTCTCATCATAGGGCGAGGGATGGAGCGTTTGAATTGAACGATGTGTTCGCAATCATCAACGCGGTGCCTGCCATTGCTCTTCTTTCTTTCGGCTTCTTCAATCAGGGCTTCTTCCCCGGTCTATGTTTTGGTGCTGGTTTGGGAATTACGGTGTTTGGGATGGCCTACATGTTCGTCCATGATGGCCTCGTGCACCGTCGTTTCCCTGTGGGTCCCATAGCTGCTGTCCCCTACTTACGACGTGTCGCTGCTGCCCATCATATCCATCATACGGACAAATTCGATGGCGTCCCGTACGGGTTGTTTTTAGGACCCAAGGAACTGGAAGACGTGGATGGCGAGGAAGAGCTGCAGAATGAAATTAAAAAGAGAAGCAAAGTTTACAGAAATTAAATTTAATTTTCTCATTATATTAGTAATAATCAACAATAGGTGGTTATTATTTTTTTAATTTTTTTCTCTTCGTGTAATTAATTATTTTTTATTAACACGAATACTCGGTTAAGCAATAAATTTAATTCATA

Coding sequence (CDS)

ATGGCGACGGGGATCTCGGCCGTTGCAACCGCGGCGCCGTACCGGAGAATAATTCAACGACCGGCGAAAGCTCCAGCTCCGGCGAACCGTGTTCCATGGCGGTGCTTTGCAAAACAGAGCACGAGAAGATTGTCGACAGTGAAGAAGAAATGTGGGGTGAGTGAAGTTGAAATGGTTAGGGATTTGAAGGTAATCGTGGAGGAGAAAATGGGGAGGAAGAAAGCGGAAAGGGATGCTTATCTTGTTGCTGCAATTGTGTCGAGCTTCGGGATCACGTCCATGGCTGCCATCGCTGTCTATTACAGATTCTCCTGGCAACTCGAGGGTGGAGATTTTCCGGTTTTGGAAATGTTTGGTACTTTCGCACTATCGGTTGGAGCTGCTGTGGGGATGGAATTCTGGGCGAGATGGGCACATAAACAACTGTGGCATGATGCGCTATGGAATATGCACAAGTCTCATCATAGGGCGAGGGATGGAGCGTTTGAATTGAACGATGTGTTCGCAATCATCAACGCGGTGCCTGCCATTGCTCTTCTTTCTTTCGGCTTCTTCAATCAGGGCTTCTTCCCCGGTCTATGTTTTGGTGCTGGTTTGGGAATTACGGTGTTTGGGATGGCCTACATGTTCGTCCATGATGGCCTCGTGCACCGTCGTTTCCCTGTGGGTCCCATAGCTGCTGTCCCCTACTTACGACGTGTCGCTGCTGCCCATCATATCCATCATACGGACAAATTCGATGGCGTCCCGTACGGGTTGTTTTTAGGACCCAAGGAACTGGAAGACGTGGATGGCGAGGAAGAGCTGCAGAATGAAATTAAAAAGAGAAGCAAAGTTTACAGAAATTAA

Protein sequence

MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVRDLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGTFALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN

Homology

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BLAST of CmaCh15G012470.1 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 1.9e-97
Identity = 167/220 (75.91%), Postives = 194/220 (88.18%), Query Frame = 0


Query: 62  LKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGTF 121
           L + + EK+ RKK+ER  YL+AA++SSFGITSMA +AVYYRFSWQ+EGG+  +LEMFGTF
Sbjct: 80  LALRLAEKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTF 139

Query: 122 ALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALLS 181
           ALSVGAAVGMEFWARWAH+ LWH +LWNMH+SHH+ R+G FELNDVFAI+NA PAI LLS
Sbjct: 140 ALSVGAAVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLS 199

Query: 182 FGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIH 241
           +GFFN+G  PGLCFGAGLGITVFG+AYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +H
Sbjct: 200 YGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQLH 259

Query: 242 HTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYR 282
           HTDKF+GVPYGLFLGPKELE+V G EEL  EI +R K Y+
Sbjct: 260 HTDKFNGVPYGLFLGPKELEEVGGNEELDKEISRRIKSYK 299

[Close]

BLAST of CmaCh15G012470.1 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 3.5e-96
Identity = 170/243 (69.96%), Postives = 204/243 (83.95%), Query Frame = 0


Query: 38  KQSTRRLSTVKKKCGVSEVEMVRDLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAI 97
           KQS+      K +   S  E++   +++  +K  +KK+ER  YL+AA++SSFGITSMA +
Sbjct: 56  KQSSPMDDDNKPESTTSSSEILMTSRLL--KKAEKKKSERFTYLIAAVMSSFGITSMAIM 115

Query: 98  AVYYRFSWQLEGGDFPVLEMFGTFALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRA 157
           AVYYRFSWQ++GG+  VLEMFGTFALSVGAAVGMEFWARWAH+ LWHD+LWNMH+SHH+ 
Sbjct: 116 AVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHKP 175

Query: 158 RDGAFELNDVFAIINAVPAIALLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVH 217
           R+GAFELNDVFAI NAVPAI LL +GF N+G  PGLCFGAGLGIT+FGMAYMFVHDGLVH
Sbjct: 176 REGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLVH 235

Query: 218 RRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRS 277
           +RFPVGPIA VPYLR+VAAAH +HHTDKF GVPYGLFLGPKE+E+V G+EEL+ EI +R 
Sbjct: 236 KRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRRI 295

Query: 278 KVY 281
           K+Y
Sbjct: 296 KLY 296

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BLAST of CmaCh15G012470.1 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 349.7 bits (896), Expect = 3.0e-95
Identity = 165/232 (71.12%), Postives = 200/232 (86.21%), Query Frame = 0


Query: 50  KCGVSEVEMVRDLKVI---VEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQ 109
           + G  ++EM  + ++    + EK+ RKK+ER  YLVAA++SSFGITSMA +AVYYRF WQ
Sbjct: 72  EAGEEDIEMKIEEQISATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQ 131

Query: 110 LEGGDFPVLEMFGTFALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELND 169
           +EGG+ P  EMFGTFALSVGAAVGMEFWARWAHK LWH +LW+MH+SHH+ R+G FELND
Sbjct: 132 MEGGEVPFSEMFGTFALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELND 191

Query: 170 VFAIINAVPAIALLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIA 229
           VFAIINAVPAIALL +GFF++G  PGLCFGAGLGITVFGMAYMFVHDGLVH+RFPVGP+A
Sbjct: 192 VFAIINAVPAIALLDYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVA 251

Query: 230 AVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSK 279
            VPYLR+VAAAH +HH++KF+GVPYGLFLGPKELE+V G EEL+ E+ +R++
Sbjct: 252 NVPYLRKVAAAHSLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRRTR 303

[Close]

BLAST of CmaCh15G012470.1 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 3.9e-95
Identity = 166/232 (71.55%), Postives = 199/232 (85.78%), Query Frame = 0


Query: 52  GVSEVEMVRDLKVI-----VEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQ 111
           G  EV   R+ ++      + +K+ RKK+ER  YLVAA++SSFGITSMA ++VYYRFSWQ
Sbjct: 85  GAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYRFSWQ 144

Query: 112 LEGGDFPVLEMFGTFALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELND 171
           +EGG+ P+ EMFGTFALSVGAAVGMEFWARWAH+ LWH +LW+MH+SHH+ R+G FELND
Sbjct: 145 MEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGPFELND 204

Query: 172 VFAIINAVPAIALLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIA 231
           +FAIINAVPAIALLS+GFF++G  PGLCFGAGLGITVFGMAYMFVHDGLVH+RFPVGPIA
Sbjct: 205 IFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIA 264

Query: 232 AVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSK 279
            VPY RRVAAAH +HH+DKF+GVPYGLFLGPKELE+V G + L+ EI +R+K
Sbjct: 265 DVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINRRTK 316

[Close]

BLAST of CmaCh15G012470.1 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 340.9 bits (873), Expect = 1.4e-92
Identity = 162/224 (72.32%), Postives = 189/224 (84.38%), Query Frame = 0


Query: 55  EVEMVRDLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPV 114
           E    + L V + EK  RKK+ER  YLVAA++SS GITSMA I+VYYRFSWQ+EGG+ P 
Sbjct: 87  ETNEEKSLAVRLAEKFARKKSERFTYLVAAVMSSLGITSMAVISVYYRFSWQMEGGEMPF 146

Query: 115 LEMFGTFALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAV 174
            EMF TFAL+ GAA+GME+WARWAH+ LWH +LW+MH+SHHR R+G FELND+FAIINAV
Sbjct: 147 SEMFCTFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFAIINAV 206

Query: 175 PAIALLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRV 234
           PAIALLSFGF ++G  PGLCFGAGLGITVFGMAYMFVHDGLVH+RFPVGPIA VPY +RV
Sbjct: 207 PAIALLSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFQRV 266

Query: 235 AAAHHIHHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSK 279
           AAAH +HH+DKFDGVPYGLFLGPKELE+V   EEL+ E+ +R K
Sbjct: 267 AAAHQLHHSDKFDGVPYGLFLGPKELEEVGVLEELEKEVNRRIK 310

[Close]

BLAST of CmaCh15G012470.1 vs. ExPASy TrEMBL
Match: A0A6J1I1U3 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469080 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 5.7e-158
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0


Query: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60
           MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR
Sbjct: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60

Query: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120
           DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT
Sbjct: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120

Query: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180
           FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL
Sbjct: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180

Query: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240
           SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI
Sbjct: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240

Query: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 283
           HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN
Sbjct: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 282

[Close]

BLAST of CmaCh15G012470.1 vs. ExPASy TrEMBL
Match: A0A6J1EQT9 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435746 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 2.9e-149
Identity = 268/282 (95.04%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60
           MATGISAVATAAPYRR+IQRPAKAP PANRVPWRCFAKQSTRRLSTVKKK GVSEVE+V+
Sbjct: 1   MATGISAVATAAPYRRLIQRPAKAPPPANRVPWRCFAKQSTRRLSTVKKKSGVSEVEIVK 60

Query: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120
           DLKVIVEEKM RKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQL+GGDFPVLEM GT
Sbjct: 61  DLKVIVEEKMVRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLKGGDFPVLEMVGT 120

Query: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180
           FALSVGAAVGMEFWARWAHKQLWHD+LWNMHKSHHRAR+G FELNDVFAIINAVPAIALL
Sbjct: 121 FALSVGAAVGMEFWARWAHKQLWHDSLWNMHKSHHRARNGPFELNDVFAIINAVPAIALL 180

Query: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240
           SFGFF+QGF PGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI
Sbjct: 181 SFGFFHQGFVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240

Query: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 283
           HHTDKFDGVPYGLFLGPKELEDVDGEEELQ EIKKRSKVYRN
Sbjct: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQKEIKKRSKVYRN 282

[Close]

BLAST of CmaCh15G012470.1 vs. ExPASy TrEMBL
Match: A0A6J1HZM0 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469080 PE=3 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.0e-142
Identity = 262/282 (92.91%), Postives = 262/282 (92.91%), Query Frame = 0


Query: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60
           MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR
Sbjct: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60

Query: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120
           DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLE            
Sbjct: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLE------------ 120

Query: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180
                   VGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL
Sbjct: 121 --------VGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180

Query: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240
           SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI
Sbjct: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240

Query: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 283
           HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN
Sbjct: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 262

[Close]

BLAST of CmaCh15G012470.1 vs. ExPASy TrEMBL
Match: A0A6J1ET27 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435746 PE=3 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.5e-134
Identity = 249/282 (88.30%), Postives = 256/282 (90.78%), Query Frame = 0


Query: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60
           MATGISAVATAAPYRR+IQRPAKAP PANRVPWRCFAKQSTRRLSTVKKK GVSEVE+V+
Sbjct: 1   MATGISAVATAAPYRRLIQRPAKAPPPANRVPWRCFAKQSTRRLSTVKKKSGVSEVEIVK 60

Query: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120
           DLKVIVEEKM RKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQL+            
Sbjct: 61  DLKVIVEEKMVRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLK------------ 120

Query: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180
                   VGMEFWARWAHKQLWHD+LWNMHKSHHRAR+G FELNDVFAIINAVPAIALL
Sbjct: 121 --------VGMEFWARWAHKQLWHDSLWNMHKSHHRARNGPFELNDVFAIINAVPAIALL 180

Query: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240
           SFGFF+QGF PGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI
Sbjct: 181 SFGFFHQGFVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240

Query: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 283
           HHTDKFDGVPYGLFLGPKELEDVDGEEELQ EIKKRSKVYRN
Sbjct: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQKEIKKRSKVYRN 262

[Close]

BLAST of CmaCh15G012470.1 vs. ExPASy TrEMBL
Match: A0A6J1I2U2 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111469080 PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 4.2e-124
Identity = 237/282 (84.04%), Postives = 237/282 (84.04%), Query Frame = 0


Query: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60
           MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR
Sbjct: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60

Query: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120
           DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT
Sbjct: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120

Query: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180
           FALSVGAAVGMEFWARWAHKQLWHDALWNMHK                            
Sbjct: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHK---------------------------- 180

Query: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240
                            GLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI
Sbjct: 181 -----------------GLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 237

Query: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 283
           HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN
Sbjct: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 237

[Close]

BLAST of CmaCh15G012470.1 vs. NCBI nr
Match: XP_022970080.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 566.6 bits (1459), Expect = 1.2e-157
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0


Query: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60
           MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR
Sbjct: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60

Query: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120
           DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT
Sbjct: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120

Query: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180
           FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL
Sbjct: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180

Query: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240
           SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI
Sbjct: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240

Query: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 283
           HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN
Sbjct: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 282

[Close]

BLAST of CmaCh15G012470.1 vs. NCBI nr
Match: XP_023550966.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023550967.1 beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 549.3 bits (1414), Expect = 2.0e-152
Identity = 273/282 (96.81%), Postives = 276/282 (97.87%), Query Frame = 0


Query: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60
           MATGISAVATAAPYRR+IQRPAKAP PANRVPWRCFAKQSTRRLS VKKK GVSEVEMV+
Sbjct: 1   MATGISAVATAAPYRRLIQRPAKAPPPANRVPWRCFAKQSTRRLSMVKKKSGVSEVEMVK 60

Query: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120
           DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQL+GGDFPVLEMFGT
Sbjct: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLKGGDFPVLEMFGT 120

Query: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180
           FALSVGAAVGMEFWARWAHKQLWHD LWNMHKSHHRARDG FELNDVFAIINAVPAIALL
Sbjct: 121 FALSVGAAVGMEFWARWAHKQLWHDTLWNMHKSHHRARDGPFELNDVFAIINAVPAIALL 180

Query: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240
           SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI
Sbjct: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240

Query: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 283
           HHTDKFDGVPYGLFLGPKELEDVDGEEELQ EIKKRSKVYRN
Sbjct: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQKEIKKRSKVYRN 282

[Close]

BLAST of CmaCh15G012470.1 vs. NCBI nr
Match: XP_022929023.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG7016987.1 Beta-carotene 3-hydroxylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 537.7 bits (1384), Expect = 5.9e-149
Identity = 268/282 (95.04%), Postives = 275/282 (97.52%), Query Frame = 0


Query: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60
           MATGISAVATAAPYRR+IQRPAKAP PANRVPWRCFAKQSTRRLSTVKKK GVSEVE+V+
Sbjct: 1   MATGISAVATAAPYRRLIQRPAKAPPPANRVPWRCFAKQSTRRLSTVKKKSGVSEVEIVK 60

Query: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120
           DLKVIVEEKM RKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQL+GGDFPVLEM GT
Sbjct: 61  DLKVIVEEKMVRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLKGGDFPVLEMVGT 120

Query: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180
           FALSVGAAVGMEFWARWAHKQLWHD+LWNMHKSHHRAR+G FELNDVFAIINAVPAIALL
Sbjct: 121 FALSVGAAVGMEFWARWAHKQLWHDSLWNMHKSHHRARNGPFELNDVFAIINAVPAIALL 180

Query: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240
           SFGFF+QGF PGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI
Sbjct: 181 SFGFFHQGFVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240

Query: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 283
           HHTDKFDGVPYGLFLGPKELEDVDGEEELQ EIKKRSKVYRN
Sbjct: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQKEIKKRSKVYRN 282

[Close]

BLAST of CmaCh15G012470.1 vs. NCBI nr
Match: KAG6579527.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 536.2 bits (1380), Expect = 1.7e-148
Identity = 267/282 (94.68%), Postives = 274/282 (97.16%), Query Frame = 0


Query: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60
           MATGISAVATAAPYRR+IQRPAKAP PANRVPWRCFAKQSTRRLSTVKKK GVSEVE+V+
Sbjct: 1   MATGISAVATAAPYRRLIQRPAKAPPPANRVPWRCFAKQSTRRLSTVKKKSGVSEVEIVK 60

Query: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120
           DLKVIVEEKM RKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQL+GGDFPVLEM GT
Sbjct: 61  DLKVIVEEKMVRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLKGGDFPVLEMVGT 120

Query: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180
           F LSVGAAVGMEFWARWAHKQLWHD+LWNMHKSHHRAR+G FELNDVFAIINAVPAIALL
Sbjct: 121 FTLSVGAAVGMEFWARWAHKQLWHDSLWNMHKSHHRARNGPFELNDVFAIINAVPAIALL 180

Query: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240
           SFGFF+QGF PGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI
Sbjct: 181 SFGFFHQGFVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240

Query: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 283
           HHTDKFDGVPYGLFLGPKELEDVDGEEELQ EIKKRSKVYRN
Sbjct: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQKEIKKRSKVYRN 282

[Close]

BLAST of CmaCh15G012470.1 vs. NCBI nr
Match: XP_022970081.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 515.0 bits (1325), Expect = 4.1e-142
Identity = 262/282 (92.91%), Postives = 262/282 (92.91%), Query Frame = 0


Query: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60
           MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR
Sbjct: 1   MATGISAVATAAPYRRIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEVEMVR 60

Query: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGT 120
           DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLE            
Sbjct: 61  DLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLE------------ 120

Query: 121 FALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180
                   VGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL
Sbjct: 121 --------VGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALL 180

Query: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240
           SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI
Sbjct: 181 SFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 240

Query: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 283
           HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN
Sbjct: 241 HHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 262

[Close]

BLAST of CmaCh15G012470.1 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 357.1 bits (915), Expect = 1.3e-98
Identity = 167/220 (75.91%), Postives = 194/220 (88.18%), Query Frame = 0


Query: 62  LKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGTF 121
           L + + EK+ RKK+ER  YL+AA++SSFGITSMA +AVYYRFSWQ+EGG+  +LEMFGTF
Sbjct: 80  LALRLAEKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTF 139

Query: 122 ALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALLS 181
           ALSVGAAVGMEFWARWAH+ LWH +LWNMH+SHH+ R+G FELNDVFAI+NA PAI LLS
Sbjct: 140 ALSVGAAVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLS 199

Query: 182 FGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIH 241
           +GFFN+G  PGLCFGAGLGITVFG+AYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +H
Sbjct: 200 YGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQLH 259

Query: 242 HTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYR 282
           HTDKF+GVPYGLFLGPKELE+V G EEL  EI +R K Y+
Sbjct: 260 HTDKFNGVPYGLFLGPKELEEVGGNEELDKEISRRIKSYK 299

[Close]

BLAST of CmaCh15G012470.1 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 352.8 bits (904), Expect = 2.5e-97
Identity = 170/243 (69.96%), Postives = 204/243 (83.95%), Query Frame = 0


Query: 38  KQSTRRLSTVKKKCGVSEVEMVRDLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAI 97
           KQS+      K +   S  E++   +++  +K  +KK+ER  YL+AA++SSFGITSMA +
Sbjct: 56  KQSSPMDDDNKPESTTSSSEILMTSRLL--KKAEKKKSERFTYLIAAVMSSFGITSMAIM 115

Query: 98  AVYYRFSWQLEGGDFPVLEMFGTFALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRA 157
           AVYYRFSWQ++GG+  VLEMFGTFALSVGAAVGMEFWARWAH+ LWHD+LWNMH+SHH+ 
Sbjct: 116 AVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHKP 175

Query: 158 RDGAFELNDVFAIINAVPAIALLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVH 217
           R+GAFELNDVFAI NAVPAI LL +GF N+G  PGLCFGAGLGIT+FGMAYMFVHDGLVH
Sbjct: 176 REGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLVH 235

Query: 218 RRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRS 277
           +RFPVGPIA VPYLR+VAAAH +HHTDKF GVPYGLFLGPKE+E+V G+EEL+ EI +R 
Sbjct: 236 KRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRRI 295

Query: 278 KVY 281
           K+Y
Sbjct: 296 KLY 296

[Close]

BLAST of CmaCh15G012470.1 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 216.1 bits (549), Expect = 3.7e-56
Identity = 100/136 (73.53%), Postives = 119/136 (87.50%), Query Frame = 0


Query: 62  LKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVLEMFGTF 121
           L + + EK+ RKK+ER  YL+AA++SSFGITSMA +AVYYRFSWQ+EGG+  +LEMFGTF
Sbjct: 80  LALRLAEKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTF 139

Query: 122 ALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALLS 181
           ALSVGAAVGMEFWARWAH+ LWH +LWNMH+SHH+ R+G FELNDVFAI+NA PAI LLS
Sbjct: 140 ALSVGAAVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLS 199

Query: 182 FGFFNQGFFPGLCFGA 198
           +GFFN+G  PGLCFGA
Sbjct: 200 YGFFNKGLVPGLCFGA 215

[Close]

BLAST of CmaCh15G012470.1 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 201.1 bits (510), Expect = 1.2e-51
Identity = 116/243 (47.74%), Postives = 143/243 (58.85%), Query Frame = 0


Query: 38  KQSTRRLSTVKKKCGVSEVEMVRDLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAI 97
           KQS+      K +   S  E++   +++  +K  +KK+ER  YL+AA++SSFGITSMA +
Sbjct: 56  KQSSPMDDDNKPESTTSSSEILMTSRLL--KKAEKKKSERFTYLIAAVMSSFGITSMAIM 115

Query: 98  AVYYRFSWQLEGGDFPVLEMFGTFALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRA 157
           AVYYRFSWQ++GG+  VLEMFGTFALSVGA                              
Sbjct: 116 AVYYRFSWQMKGGEVSVLEMFGTFALSVGA------------------------------ 175

Query: 158 RDGAFELNDVFAIINAVPAIALLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVH 217
                                                  AGLGIT+FGMAYMFVHDGLVH
Sbjct: 176 ---------------------------------------AGLGITMFGMAYMFVHDGLVH 227

Query: 218 RRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRS 277
           +RFPVGPIA VPYLR+VAAAH +HHTDKF GVPYGLFLGPKE+E+V G+EEL+ EI +R 
Sbjct: 236 KRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRRI 227

Query: 278 KVY 281
           K+Y
Sbjct: 296 KLY 227

The following BLAST results are available for this feature:

BLAST of CmaCh15G012470.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZZ8	1.9e-97	75.91	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9LTG0	3.5e-96	69.96	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O49814	3.0e-95	71.12	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
B3SGL0	3.9e-95	71.55	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
O49815	1.4e-92	72.32	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]

BLAST of CmaCh15G012470.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I1U3	5.7e-158	100.00	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita maxima...	[more]
A0A6J1EQT9	2.9e-149	95.04	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita moscha...	[more]
A0A6J1HZM0	2.0e-142	92.91	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 OS=Cucurbita maxima...	[more]
A0A6J1ET27	1.5e-134	88.30	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 OS=Cucurbita moscha...	[more]
A0A6J1I2U2	4.2e-124	84.04	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X3 OS=Cucurbita maxima...	[more]

BLAST of CmaCh15G012470.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022970080.1	1.2e-157	100.00	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Cucurbita maxima]	[more]
XP_023550966.1	2.0e-152	96.81	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Cucurbita pepo sub...	[more]
XP_022929023.1	5.9e-149	95.04	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Cucurbita moschata...	[more]
KAG6579527.1	1.7e-148	94.68	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_022970081.1	4.1e-142	92.91	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 [Cucurbita maxima]	[more]

BLAST of CmaCh15G012470.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.1	1.3e-98	75.91	beta-hydroxylase 1	[more]
AT5G52570.1	2.5e-97	69.96	beta-carotene hydroxylase 2	[more]
AT4G25700.2	3.7e-56	73.53	beta-hydroxylase 1	[more]
AT5G52570.2	1.2e-51	47.74	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 127..254 e-value: 3.0E-13 score: 50.3
None	No IPR available	PANTHER	PTHR31899:SF14	HYDROXYLASE, PUTATIVE, EXPRESSED-RELATED	coord: 10..278
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 10..278

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh15G012470	CmaCh15G012470	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh15G012470.1:exon:1063	CmaCh15G012470.1:exon:1063	exon
CmaCh15G012470.1:exon:1062	CmaCh15G012470.1:exon:1062	exon
CmaCh15G012470.1:exon:1061	CmaCh15G012470.1:exon:1061	exon
CmaCh15G012470.1:exon:1060	CmaCh15G012470.1:exon:1060	exon
CmaCh15G012470.1:exon:1059	CmaCh15G012470.1:exon:1059	exon
CmaCh15G012470.1:exon:1058	CmaCh15G012470.1:exon:1058	exon
CmaCh15G012470.1:exon:1057	CmaCh15G012470.1:exon:1057	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh15G012470.1:three_prime_utr	CmaCh15G012470.1:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh15G012470.1:cds	CmaCh15G012470.1:cds_7	CDS
CmaCh15G012470.1:cds	CmaCh15G012470.1:cds_6	CDS
CmaCh15G012470.1:cds	CmaCh15G012470.1:cds_5	CDS
CmaCh15G012470.1:cds	CmaCh15G012470.1:cds_4	CDS
CmaCh15G012470.1:cds	CmaCh15G012470.1:cds_3	CDS
CmaCh15G012470.1:cds	CmaCh15G012470.1:cds_2	CDS
CmaCh15G012470.1:cds	CmaCh15G012470.1:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh15G012470.1:five_prime_utr	CmaCh15G012470.1:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh15G012470.1	CmaCh15G012470.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity