CmaCh14G004640.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G004640.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description14 kDa zinc-binding protein
LocationCma_Chr14: 2259996 .. 2264545 (+)
Sequence length699
RNA-Seq ExpressionCmaCh14G004640.1
SyntenyCmaCh14G004640.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGATGAAGATATAAAGCACTGGACTCGACTCTCAGTTCTAAGCTCCCACATTTTCCAATCTCTTTCCATTTCCATGGCATCTTCCGAGAAGGAAGCGGCTCTTGCAGCTGTTCCCTCTGATTCCCCCACCATGTAAGCTTCTTTAAGCGCCGTTGCCAACCAACATTCTGTGTTTTTAGTTCTATATACTCTTTAATTTGTCTTTTTTGTGTGTGTGTTTGATTTGCTGGTAATCGGTTCTTGATTGTTTCATGGCGATTGAGAAATCGGGGAATGGAATTGTTGATTCTTCTTATAATTATGTATTATTTTGGTTGTTTAGTGCATGTAGCGTATGCTCAAAGCTGAAGGCTAATGGTTCTGTGAGTACGGCGAGATTTAAAGATGTTTAAAGATTTTGAACTGTACAAATTGACGGGAGTTGGGTTCAATGTTGGGGAAAATAATCGCTGTAAGGAGACATCTATGAGAGGGTAATCGAAATTGCGGTAGCTTATTATAGAAAAAAAGAAACAAATATTTTTTTTTGGCATTAGTTGCTTTTGATTGCTTGGAAGGTGAGAAATGGACGAAGTGGTTAAAAATTTAATGAAAAAATCCCCACACAATAACTTGGAGCAACCAATGAAGAGCCGGAGATCGCTTTCACTATATGCATTTTCGTGCCTTAGGAAATAACGGATTGATTGATCTGCCGAACTTACTATTCTTAAGCAAAACTATCTGAACATGGTGGAATTTCTTGAATCCGAAGACCACAAATGTCAAGGAAGGAAGAGAATTTATTTAAACCCGAAGGGAGTGAAGGACAAGCTTGTTTTGCATACTTAATGGAAGAAATTGGGATAGCCTCACCACTATTTTTTGAAGTTGCATCCTTATTTTCCGCATTAAACAACAAACAGAGAGGTTTTTTGCAAGAATCTTACACAAAGACATCATAGGAAAAACATTGTGTCTAGTTTAGTGTGCTTTTGTTGAAGGGAAACTAGTTACTTGAGGAAGTAATGAACAAAATATAGTTGCTACGATTATGAGTAATATTGAAGTAGAATTTTGGGATTTTCCTTTGGAATTTGTGAGAGTAGATGAAATTTGTGAAGAGGTACAATCTTCCTAGTTGGTTTCCTGTAAGGATATACTATGATGAACATACAAATATTTAAATGTTTATAGTTCATAAGGATAAAAAAACCTTGAAGTGGACAATGAACAACGTCAAGGAAAAGCTTGATGTATGAGTCATATGTAAGTTTCCTACAACAAAACAAATTGCTAATGTATTATTTGAGTGACTATACAAAAAACAATCTGACGAATTAATTGTCAATCTGGCCATGCAAAAGTCTTCAAATATGCATGAGGTGGAGTACTTGATTTGTTTATATTGTTTATCTTTTGTTTATTATTGACAGGGAAAATTCAAATTATACCTTGTCCAATAACTTAGGCCCTTAAGTTAAATAAATCAATTAAGTATTTGAATGTGTGTGTGTGTTTGTGTCTTGGAGATTTGTGGCTCAATTTGAGTACTTTTGTTGGCCAGATAATACTATTGTTGTTGGAATTGATTCTTCGGGTCATTACGAGATTCTTTCCATTGTAATTTTAATATTTTTTCATTAGATTTAGCACTATTTTAATAACTTAAGGTTGTTGCTTAGTTTGGTATTTTTATTATTCAGTATTGCTATTTTTGTGGTTTGATATTGAATATTTTGTTAGTGTTAAATTTCATTGGATTTATTCGTTAGTTCATGATTATATTTTTTATTTAGTTTTGAAGAGGCATTAATTGCACAATTTAGTTAAGTTTGAGATTGATGAAATTGAAAGCTGTAGGTTTTGACATACCAAGCGAAGTAAGGTAGTAAATTGGATTTTTTTTCTTATACGAATAAGTACTCATTGCTGCATATTAGGGAAGTAGCTATCAATTTTTATTTTTACTTCTTTCCTTTTGGAGTTTGTTCTTTATCCCCTTTGATAAAATCATTACATTTCACGAAAACCCATTTCAAGTTGCAACTAATTTTTTTGGGTTGTTACGTTTGCAGATTTGACAAAATCATTAATAAGGAAATTCCATCTACGATTGTCTTTGAGGATGATAAGGTTCCTTTACATTATTACTATATAATTTGTATTATTATGTAAAAGAATTTTAGAGGGAAGGAAATATTAAGTTTGGACAATGGCCTATCTAGATTAATTTTGTGAATAATGTATTTGCTGAATTTGTTATATTACTTCTAGAGAGAAATTGTTACTATTGCATGGTGGAAATACGATGCTGGTACCCATCATTGATATGACGCTAGCATTGATAAAGACTTGTCTCTCTTTAAAATTCTTCAGTAATGGAAAGGTACCCTTCGTCCAACTTGATGGATCCCTATTTGAACTAAGATTGACAGACCTTTTGTTTTCTCCACTTGGGCACATATTCATGGAAGGCTGGGTTGAAAGATTGACAGACCGTTTTTCTTTTTTGATTAAATTTTTTTGTGATTATTTCTTCAAATCAGTTCCTTGGTTTCTTAATTTTGTCCCAAGGATTTAATGTATATTTATTATGTATCCTTCAATGTCCTTTCAATTACGACCGCTCATTTCGTTTTATAAATCTTGCTTGGATGGTGTCATGAGATGAATACTTGGAATTGAAGTTAAGCTCTGTCATTGTGAGTTCTATTAATTTTTGTTTCAATTTCACTCTGATGGCAGGTTCTTGCTTTTAGGGACATATCTCCACAAGCTCCTACACATATTCTAATCATTCCAAAAGTTAAGGATGGGTTATCTGGATTATCTAAGGTACTTCTTTACTGGGTATTTTAGTCTCTGCTTTTAAGCATCCGTCGACTTGGTTCTTCTAGCATTTGCATTTTTGAAGTCATTTTGATCTTATTATATGAAATATCCACTATATGCTTGAATAATGATTGAGAAGTTAGATTTATCTTTATAGTGGAGAAATTTGAGTGGCAATTAGATTGTCTAACCAGAGTAAGAAAATCTCTAGTAGAGGTGTTTACTATTTGGTTTGGATGTAGTTTAGGGACTTTTCCAGCCCTACTCAATGACTGTAGTTAGATGATGGTCCCAATTCAAGTTATTCAACCTTAGTTTGAGTTGTTCAATCAAGTCATGTAATCAATTTTGTAACAGTAATTGTGGAAATGTTAGTATTTTATGTATTTATTTAACTATTTAGTTTATTCACACCAATTGAATTTGGTTACTCACTTTCATTTTCTATTTTACGGAAAATACACTCGAGGAACTTGTGAATTATTTTCCACAAAATTTTGCCTGTTGGTGGGTAAAGATTTAAAAGAAATTGATTGAATACAATTTAAATAAATACTGCATAAGTAATTTTATTATGAGTAAAAGAGATTTTTGTAATTAATTAGGCCCCATATGGTAGCCATTTAGTTTTTTGTTTTTGAAAATTATGCACACTATTACCAGTGAGATTTTTTTTTTTTTTTTTAGTTATCTACTTTCTAATCATATTCTCAAAATTCATGTCAAATTTTGAAACTAAAAAAGTGTAGGTTTTTTAAAAAAATTATTTTTGGACTTTGATAAGAGTTCAAATGTCACCTTAAGAACGATAAAGGCCATTGTAAAGAAATGGTGAAAAAACCAAAAAGAAAAAATCGAATGACAAAGATTTTTGATACTTGAAAGGCGTATTATGGTCTTTGGATTACAATGAGTGGGCATCTAAAATATATGAAGCTAAACTTTTAAGAACTTAAGAAATGTTTCTCAGTCTTCACTAAAACCGTTACATAATTGCGTCTCTATATGTTAAGGAATTTTGTAGTTGGAATTTGTGTTTGTGTTGAACTGTTGATTTTAATGGCCCGTTCTAGTTTTATGCTGTTATGAGGTTGAAACTTATCCATTGACGCTAAAAGGATTATTATATTTATTAGACACTGGCTCAGGGGCTGTATTGTTCATTCATAGATATTATTGTCCTTTATTTGCATTTTCATTGTATATTTCTTGTGCTTTGTAGGCTGAGGATAGGCACACAGAGATTCTCGGTCAACTTCTTTACGTTGCCAAGCTCATTGCCAAACAAGAAGGGCTGGACGATGGCTTTAGGGTCGTAATTAATGATGGACCGAGTGGATGTTAGTACTCAAATCCCCATCTTTTACACTCGATAACAAATTTCTTCTCTTATTATTAAAAGTATCAAGCATTTGATTCTGTCATTTTCTCGCAGGCCAATCGGTTTATCATCTTCATGTTCACCTTTTGGGGGGACGACAAATGAATTGGTCCCCAGGTTAAGATGGACACTTTTTCAACGTTGAAATACATCTAATAATGTTTTCATTTACCTGTTCGAAAACTTTATCAACTTTATCAACTACTGCTCGTCCCTTGTGGTCTCTAACATCCCATTGAATATGGTAATTGATATAAGTTTGGTGACTATTGTTGTTTTTCTTGAATATCTCATTGAAAATGGATGGCAATGTAATGTATGCGGTGGCTCTGTCTCAATGGCAGTAAGAA

mRNA sequence

ATGGTGGATGAAGATATAAAGCACTGGACTCGACTCTCAGTTCTAAGCTCCCACATTTTCCAATCTCTTTCCATTTCCATGGCATCTTCCGAGAAGGAAGCGGCTCTTGCAGCTGTTCCCTCTGATTCCCCCACCATATTTGACAAAATCATTAATAAGGAAATTCCATCTACGATTGTCTTTGAGGATGATAAGGTTCTTGCTTTTAGGGACATATCTCCACAAGCTCCTACACATATTCTAATCATTCCAAAAGTTAAGGATGGGTTATCTGGATTATCTAAGGCTGAGGATAGGCACACAGAGATTCTCGGTCAACTTCTTTACGTTGCCAAGCTCATTGCCAAACAAGAAGGGCTGGACGATGGCTTTAGGGTCGTAATTAATGATGGACCGAGTGGATGCCAATCGGTTTATCATCTTCATGTTCACCTTTTGGGGGGACGACAAATGAATTGGTCCCCAGGTTAAGATGGACACTTTTTCAACGTTGAAATACATCTAATAATGTTTTCATTTACCTGTTCGAAAACTTTATCAACTTTATCAACTACTGCTCGTCCCTTGTGGTCTCTAACATCCCATTGAATATGGTAATTGATATAAGTTTGGTGACTATTGTTGTTTTTCTTGAATATCTCATTGAAAATGGATGGCAATGTAATGTATGCGGTGGCTCTGTCTCAATGGCAGTAAGAA

Coding sequence (CDS)

ATGGTGGATGAAGATATAAAGCACTGGACTCGACTCTCAGTTCTAAGCTCCCACATTTTCCAATCTCTTTCCATTTCCATGGCATCTTCCGAGAAGGAAGCGGCTCTTGCAGCTGTTCCCTCTGATTCCCCCACCATATTTGACAAAATCATTAATAAGGAAATTCCATCTACGATTGTCTTTGAGGATGATAAGGTTCTTGCTTTTAGGGACATATCTCCACAAGCTCCTACACATATTCTAATCATTCCAAAAGTTAAGGATGGGTTATCTGGATTATCTAAGGCTGAGGATAGGCACACAGAGATTCTCGGTCAACTTCTTTACGTTGCCAAGCTCATTGCCAAACAAGAAGGGCTGGACGATGGCTTTAGGGTCGTAATTAATGATGGACCGAGTGGATGCCAATCGGTTTATCATCTTCATGTTCACCTTTTGGGGGGACGACAAATGAATTGGTCCCCAGGTTAA

Protein sequence

MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIVFEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGLDDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG
Homology
BLAST of CmaCh14G004640.1 vs. ExPASy Swiss-Prot
Match: Q8GUN2 (Adenylylsulfatase HINT1 OS=Arabidopsis thaliana OX=3702 GN=HINT1 PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.2e-61
Identity = 115/146 (78.77%), Postives = 131/146 (89.73%), Query Frame = 0

Query: 11  RLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIVFEDDKVLAFR 70
           R+S+LSSH F   S  MA SEKEAALAA PSDSPTIFDKII+KEIPST+VFEDDKVLAFR
Sbjct: 4   RVSILSSH-FSPASAVMA-SEKEAALAATPSDSPTIFDKIISKEIPSTVVFEDDKVLAFR 63

Query: 71  DISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGLDDGFRVVIND 130
           DI+PQ P HIL+IPKV+DGL+GLSKAE+RH +ILG+LLY AKL+AKQEGL +GFR+VIND
Sbjct: 64  DITPQGPVHILLIPKVRDGLTGLSKAEERHIDILGRLLYTAKLVAKQEGLAEGFRIVIND 123

Query: 131 GPSGCQSVYHLHVHLLGGRQMNWSPG 157
           GP GCQSVYH+HVHL+GGRQMNW PG
Sbjct: 124 GPQGCQSVYHIHVHLIGGRQMNWPPG 147

BLAST of CmaCh14G004640.1 vs. ExPASy Swiss-Prot
Match: P42856 (14 kDa zinc-binding protein OS=Zea mays OX=4577 GN=ZBP14 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 4.7e-58
Identity = 107/128 (83.59%), Postives = 120/128 (93.75%), Query Frame = 0

Query: 29  SSEKEAALAAVPSDSPTIFDKIINKEIPSTIVFEDDKVLAFRDISPQAPTHILIIPKVKD 88
           SSEKEAAL  +  DSPTIFDKII KEIPST+V+ED+KVLAFRDI+PQAPTHILIIPKVKD
Sbjct: 2   SSEKEAALRRL-DDSPTIFDKIIKKEIPSTVVYEDEKVLAFRDINPQAPTHILIIPKVKD 61

Query: 89  GLSGLSKAEDRHTEILGQLLYVAKLIAKQEGLDDGFRVVINDGPSGCQSVYHLHVHLLGG 148
           GL+GL+KAE+RH EILG LLYVAK++AKQEGL+DG+RVVINDGPSGCQSVYH+HVHLLGG
Sbjct: 62  GLTGLAKAEERHIEILGYLLYVAKVVAKQEGLEDGYRVVINDGPSGCQSVYHIHVHLLGG 121

Query: 149 RQMNWSPG 157
           RQMNW PG
Sbjct: 122 RQMNWPPG 128

BLAST of CmaCh14G004640.1 vs. ExPASy Swiss-Prot
Match: P42855 (14 kDa zinc-binding protein (Fragment) OS=Brassica juncea OX=3707 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 5.6e-51
Identity = 89/112 (79.46%), Postives = 104/112 (92.86%), Query Frame = 0

Query: 45  TIFDKIINKEIPSTIVFEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEIL 104
           TIF KII+KEIPST+V+EDDKVLAFRDI+PQ P HIL+IPKV+DGL+GL KAE+RH +IL
Sbjct: 2   TIFGKIISKEIPSTVVYEDDKVLAFRDITPQGPVHILLIPKVRDGLTGLFKAEERHIDIL 61

Query: 105 GQLLYVAKLIAKQEGLDDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           G+LLY AKL+AKQEGLD+GFR+VINDGP GCQSVYH+HVHL+GGRQMNW PG
Sbjct: 62  GRLLYTAKLVAKQEGLDEGFRIVINDGPQGCQSVYHIHVHLIGGRQMNWPPG 113

BLAST of CmaCh14G004640.1 vs. ExPASy Swiss-Prot
Match: Q8SQ21 (Histidine triad nucleotide-binding protein 2, mitochondrial OS=Bos taurus OX=9913 GN=HINT2 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.4e-33
Identity = 66/124 (53.23%), Postives = 91/124 (73.39%), Query Frame = 0

Query: 33  EAALAAVPSDSPTIFDKIINKEIPSTIVFEDDKVLAFRDISPQAPTHILIIPKVKDGLSG 92
           +A  AA    +PTIF +I+++ +P+ I++ED + LAFRD++PQAP H L+IP  K  +  
Sbjct: 42  KAQQAAPGGAAPTIFSRILDRSLPADILYEDQQCLAFRDVAPQAPVHFLVIP--KKPIPR 101

Query: 93  LSKAEDRHTEILGQLLYVAKLIAKQEGLDDGFRVVINDGPSGCQSVYHLHVHLLGGRQMN 152
           +S+AE+   ++LG LL VAK  AK EGL DG+R+VINDG  G QSVYHLH+H+LGGRQ+ 
Sbjct: 102 ISQAEEEDQQLLGHLLLVAKETAKAEGLGDGYRLVINDGKLGAQSVYHLHIHVLGGRQLQ 161

Query: 153 WSPG 157
           W PG
Sbjct: 162 WPPG 163

BLAST of CmaCh14G004640.1 vs. ExPASy Swiss-Prot
Match: Q9BX68 (Histidine triad nucleotide-binding protein 2, mitochondrial OS=Homo sapiens OX=9606 GN=HINT2 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 4.0e-33
Identity = 67/128 (52.34%), Postives = 91/128 (71.09%), Query Frame = 0

Query: 30  SEKEAALAAVPSD-SPTIFDKIINKEIPSTIVFEDDKVLAFRDISPQAPTHILIIPKVKD 89
           +E   A  A P   +PTIF +I++K +P+ I++ED + L FRD++PQAP H L+IP  K 
Sbjct: 38  NEVAKAQQATPGGAAPTIFSRILDKSLPADILYEDQQCLVFRDVAPQAPVHFLVIP--KK 97

Query: 90  GLSGLSKAEDRHTEILGQLLYVAKLIAKQEGLDDGFRVVINDGPSGCQSVYHLHVHLLGG 149
            +  +S+AE+   ++LG LL VAK  AK EGL DG+R+VINDG  G QSVYHLH+H+LGG
Sbjct: 98  PIPRISQAEEEDQQLLGHLLLVAKQTAKAEGLGDGYRLVINDGKLGAQSVYHLHIHVLGG 157

Query: 150 RQMNWSPG 157
           RQ+ W PG
Sbjct: 158 RQLQWPPG 163

BLAST of CmaCh14G004640.1 vs. ExPASy TrEMBL
Match: A0A6J1J6X6 (14 kDa zinc-binding protein OS=Cucurbita maxima OX=3661 GN=LOC111481782 PE=4 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 2.8e-82
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60
           MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV
Sbjct: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60

Query: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120
           FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL
Sbjct: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120

Query: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG
Sbjct: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 156

BLAST of CmaCh14G004640.1 vs. ExPASy TrEMBL
Match: A0A6J1F2T2 (14 kDa zinc-binding protein-like OS=Cucurbita moschata OX=3662 GN=LOC111441626 PE=4 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 7.2e-78
Identity = 149/156 (95.51%), Postives = 152/156 (97.44%), Query Frame = 0

Query: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60
           MV+E IKHWTRL+VLSSHI QSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV
Sbjct: 1   MVNEYIKHWTRLTVLSSHISQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60

Query: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120
           FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAE+RHTEILGQLLY AKLIAKQEGL
Sbjct: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEERHTEILGQLLYAAKLIAKQEGL 120

Query: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNW PG
Sbjct: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWPPG 156

BLAST of CmaCh14G004640.1 vs. ExPASy TrEMBL
Match: A0A6J1I2W3 (14 kDa zinc-binding protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470045 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.6e-75
Identity = 144/156 (92.31%), Postives = 149/156 (95.51%), Query Frame = 0

Query: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60
           MV+ DIK WTRLSVLSSHI  SLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPST+V
Sbjct: 53  MVNGDIKPWTRLSVLSSHITPSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTVV 112

Query: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120
           FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAE+RHTEILG LLY AKLIA+QEGL
Sbjct: 113 FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEERHTEILGHLLYTAKLIAEQEGL 172

Query: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           DDGFR+VINDGPSGCQSVYHLHVHLLGGRQMNW PG
Sbjct: 173 DDGFRIVINDGPSGCQSVYHLHVHLLGGRQMNWPPG 208

BLAST of CmaCh14G004640.1 vs. ExPASy TrEMBL
Match: A0A6J1GGA0 (14 kDa zinc-binding protein-like OS=Cucurbita moschata OX=3662 GN=LOC111453883 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.3e-74
Identity = 143/156 (91.67%), Postives = 148/156 (94.87%), Query Frame = 0

Query: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60
           MV+ DIK WTRLSVLSSHI  SLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPST+V
Sbjct: 1   MVNGDIKPWTRLSVLSSHITPSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTVV 60

Query: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120
           FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAE+RH EILG LLY AKLIA+QEGL
Sbjct: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEERHMEILGHLLYTAKLIAEQEGL 120

Query: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           DDGFR+VINDGPSGCQSVYHLHVHLLGGRQMNW PG
Sbjct: 121 DDGFRIVINDGPSGCQSVYHLHVHLLGGRQMNWPPG 156

BLAST of CmaCh14G004640.1 vs. ExPASy TrEMBL
Match: A0A1S3B859 (14 kDa zinc-binding protein OS=Cucumis melo OX=3656 GN=LOC103486830 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 6.3e-74
Identity = 143/156 (91.67%), Postives = 149/156 (95.51%), Query Frame = 0

Query: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60
           MV+EDIK  TRLSVLSSHI  SLSISMASSE+EAALAAVPSDSPTIFDKIINKEIPST+V
Sbjct: 1   MVNEDIKLRTRLSVLSSHISHSLSISMASSEQEAALAAVPSDSPTIFDKIINKEIPSTVV 60

Query: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120
           FEDDKVLAFRDI+PQAPTHILIIP+VKDGLSGLSKAE+RHTEILG LLY AKLIAKQEGL
Sbjct: 61  FEDDKVLAFRDIAPQAPTHILIIPRVKDGLSGLSKAEERHTEILGHLLYTAKLIAKQEGL 120

Query: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNW PG
Sbjct: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWPPG 156

BLAST of CmaCh14G004640.1 vs. NCBI nr
Match: XP_022983143.1 (14 kDa zinc-binding protein [Cucurbita maxima])

HSP 1 Score: 314.3 bits (804), Expect = 5.9e-82
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60
           MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV
Sbjct: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60

Query: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120
           FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL
Sbjct: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120

Query: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG
Sbjct: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 156

BLAST of CmaCh14G004640.1 vs. NCBI nr
Match: KAG7017601.1 (Adenylylsulfatase HINT1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 304.3 bits (778), Expect = 6.1e-79
Identity = 151/156 (96.79%), Postives = 153/156 (98.08%), Query Frame = 0

Query: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60
           MV+EDIKHWTRLSVLSSHI QSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV
Sbjct: 1   MVNEDIKHWTRLSVLSSHISQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60

Query: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120
           FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAE+RHTEILGQLLY AKLIAKQEGL
Sbjct: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEERHTEILGQLLYAAKLIAKQEGL 120

Query: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNW PG
Sbjct: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWPPG 156

BLAST of CmaCh14G004640.1 vs. NCBI nr
Match: XP_023521241.1 (14 kDa zinc-binding protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.0 bits (772), Expect = 3.0e-78
Identity = 149/156 (95.51%), Postives = 153/156 (98.08%), Query Frame = 0

Query: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60
           MV+EDIKHWTRL+VLSSHI QSLSISMASSEK+AALAAVPSDSPTIFDKIINKEIPSTIV
Sbjct: 1   MVNEDIKHWTRLTVLSSHISQSLSISMASSEKDAALAAVPSDSPTIFDKIINKEIPSTIV 60

Query: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120
           FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAE+RHTEILGQLLY AKLIAKQEGL
Sbjct: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEERHTEILGQLLYAAKLIAKQEGL 120

Query: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNW PG
Sbjct: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWPPG 156

BLAST of CmaCh14G004640.1 vs. NCBI nr
Match: XP_022934452.1 (14 kDa zinc-binding protein-like [Cucurbita moschata])

HSP 1 Score: 299.7 bits (766), Expect = 1.5e-77
Identity = 149/156 (95.51%), Postives = 152/156 (97.44%), Query Frame = 0

Query: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60
           MV+E IKHWTRL+VLSSHI QSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV
Sbjct: 1   MVNEYIKHWTRLTVLSSHISQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60

Query: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120
           FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAE+RHTEILGQLLY AKLIAKQEGL
Sbjct: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEERHTEILGQLLYAAKLIAKQEGL 120

Query: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNW PG
Sbjct: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWPPG 156

BLAST of CmaCh14G004640.1 vs. NCBI nr
Match: XP_022971276.1 (14 kDa zinc-binding protein-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 291.2 bits (744), Expect = 5.3e-75
Identity = 144/156 (92.31%), Postives = 149/156 (95.51%), Query Frame = 0

Query: 1   MVDEDIKHWTRLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIV 60
           MV+ DIK WTRLSVLSSHI  SLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPST+V
Sbjct: 53  MVNGDIKPWTRLSVLSSHITPSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTVV 112

Query: 61  FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGL 120
           FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAE+RHTEILG LLY AKLIA+QEGL
Sbjct: 113 FEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEERHTEILGHLLYTAKLIAEQEGL 172

Query: 121 DDGFRVVINDGPSGCQSVYHLHVHLLGGRQMNWSPG 157
           DDGFR+VINDGPSGCQSVYHLHVHLLGGRQMNW PG
Sbjct: 173 DDGFRIVINDGPSGCQSVYHLHVHLLGGRQMNWPPG 208

BLAST of CmaCh14G004640.1 vs. TAIR 10
Match: AT3G56490.1 (HIS triad family protein 3 )

HSP 1 Score: 237.3 bits (604), Expect = 8.5e-63
Identity = 115/146 (78.77%), Postives = 131/146 (89.73%), Query Frame = 0

Query: 11  RLSVLSSHIFQSLSISMASSEKEAALAAVPSDSPTIFDKIINKEIPSTIVFEDDKVLAFR 70
           R+S+LSSH F   S  MA SEKEAALAA PSDSPTIFDKII+KEIPST+VFEDDKVLAFR
Sbjct: 4   RVSILSSH-FSPASAVMA-SEKEAALAATPSDSPTIFDKIISKEIPSTVVFEDDKVLAFR 63

Query: 71  DISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGLDDGFRVVIND 130
           DI+PQ P HIL+IPKV+DGL+GLSKAE+RH +ILG+LLY AKL+AKQEGL +GFR+VIND
Sbjct: 64  DITPQGPVHILLIPKVRDGLTGLSKAEERHIDILGRLLYTAKLVAKQEGLAEGFRIVIND 123

Query: 131 GPSGCQSVYHLHVHLLGGRQMNWSPG 157
           GP GCQSVYH+HVHL+GGRQMNW PG
Sbjct: 124 GPQGCQSVYHIHVHLIGGRQMNWPPG 147

BLAST of CmaCh14G004640.1 vs. TAIR 10
Match: AT1G31160.1 (HISTIDINE TRIAD NUCLEOTIDE-BINDING 2 )

HSP 1 Score: 182.6 bits (462), Expect = 2.5e-46
Identity = 86/136 (63.24%), Postives = 109/136 (80.15%), Query Frame = 0

Query: 24  SISMASSEKEAALAA---VPSDSPTIFDKIINKEIPSTIVFEDDKVLAFRDISPQAPTHI 83
           S   A +E+ AA AA     + +PTIFDKII KEIPS IV+ED+ VLAFRDI+PQAP H+
Sbjct: 52  STRAAHNEEAAAKAAASVADTGAPTIFDKIIAKEIPSDIVYEDENVLAFRDINPQAPVHV 111

Query: 84  LIIPKVKDGLSGLSKAEDRHTEILGQLLYVAKLIAKQEGLDDGFRVVINDGPSGCQSVYH 143
           L+IPK++DGL+ L KAE RH E+LGQLL+ +K++A++EG+ DGFRVVIN+G   CQSVYH
Sbjct: 112 LVIPKLRDGLTSLGKAEPRHVEVLGQLLHASKIVAEKEGILDGFRVVINNGVEACQSVYH 171

Query: 144 LHVHLLGGRQMNWSPG 157
           LH+H+LGGRQM W PG
Sbjct: 172 LHLHVLGGRQMKWPPG 187

BLAST of CmaCh14G004640.1 vs. TAIR 10
Match: AT4G16566.1 (histidine triad nucleotide-binding 4 )

HSP 1 Score: 49.7 bits (117), Expect = 2.5e-06
Identity = 28/99 (28.28%), Postives = 48/99 (48.48%), Query Frame = 0

Query: 46  IFDKIINKEIPSTIVFEDDKVLAFRDISPQAPTHILIIPKVKDGLSGLSKAEDRHTEILG 105
           IF +I+     + ++  D+KV+AF+DI P A  H L+IPK         +  D    ++ 
Sbjct: 9   IFCEIVRNPTTTRLLHTDEKVIAFQDIKPAAQRHYLVIPKEHIPTVNDLQRRDEDYSLVR 68

Query: 106 QLLYVAKLIAKQEGLDDGFRVVINDGPSGCQSVYHLHVH 145
            +L V + + +++      R   +  P    SV HLH+H
Sbjct: 69  HMLSVGQQLLQKDAPQSIHRFGFHQPP--FNSVDHLHLH 105

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GUN21.2e-6178.77Adenylylsulfatase HINT1 OS=Arabidopsis thaliana OX=3702 GN=HINT1 PE=1 SV=1[more]
P428564.7e-5883.5914 kDa zinc-binding protein OS=Zea mays OX=4577 GN=ZBP14 PE=1 SV=1[more]
P428555.6e-5179.4614 kDa zinc-binding protein (Fragment) OS=Brassica juncea OX=3707 PE=2 SV=1[more]
Q8SQ211.4e-3353.23Histidine triad nucleotide-binding protein 2, mitochondrial OS=Bos taurus OX=991... [more]
Q9BX684.0e-3352.34Histidine triad nucleotide-binding protein 2, mitochondrial OS=Homo sapiens OX=9... [more]
Match NameE-valueIdentityDescription
A0A6J1J6X62.8e-82100.0014 kDa zinc-binding protein OS=Cucurbita maxima OX=3661 GN=LOC111481782 PE=4 SV=... [more]
A0A6J1F2T27.2e-7895.5114 kDa zinc-binding protein-like OS=Cucurbita moschata OX=3662 GN=LOC111441626 P... [more]
A0A6J1I2W32.6e-7592.3114 kDa zinc-binding protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GGA01.3e-7491.6714 kDa zinc-binding protein-like OS=Cucurbita moschata OX=3662 GN=LOC111453883 P... [more]
A0A1S3B8596.3e-7491.6714 kDa zinc-binding protein OS=Cucumis melo OX=3656 GN=LOC103486830 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022983143.15.9e-82100.0014 kDa zinc-binding protein [Cucurbita maxima][more]
KAG7017601.16.1e-7996.79Adenylylsulfatase HINT1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023521241.13.0e-7895.5114 kDa zinc-binding protein-like [Cucurbita pepo subsp. pepo][more]
XP_022934452.11.5e-7795.5114 kDa zinc-binding protein-like [Cucurbita moschata][more]
XP_022971276.15.3e-7592.3114 kDa zinc-binding protein-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G56490.18.5e-6378.77HIS triad family protein 3 [more]
AT1G31160.12.5e-4663.24HISTIDINE TRIAD NUCLEOTIDE-BINDING 2 [more]
AT4G16566.12.5e-0628.28histidine triad nucleotide-binding 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001310Histidine triad (HIT) proteinPRINTSPR00332HISTRIADcoord: 67..85
score: 67.37
coord: 46..62
score: 57.41
coord: 136..146
score: 62.55
IPR001310Histidine triad (HIT) proteinPFAMPF01230HITcoord: 53..150
e-value: 2.2E-29
score: 102.1
IPR036265HIT-like superfamilyGENE3D3.30.428.10coord: 25..156
e-value: 2.1E-41
score: 143.1
IPR036265HIT-like superfamilySUPERFAMILY54197HIT-likecoord: 42..155
NoneNo IPR availablePANTHERPTHR23089HISTIDINE TRIAD HIT PROTEINcoord: 22..156
NoneNo IPR availablePANTHERPTHR23089:SF40ADENYLYLSULFATASE HINT1coord: 22..156
NoneNo IPR availableCDDcd01276PKCI_relatedcoord: 44..148
e-value: 1.04459E-53
score: 163.116
IPR019808Histidine triad, conserved sitePROSITEPS00892HIT_1coord: 129..147
IPR011146HIT-like domainPROSITEPS51084HIT_2coord: 46..156
score: 15.831171

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh14G004640CmaCh14G004640gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh14G004640.1:exon:2596CmaCh14G004640.1:exon:2596exon
CmaCh14G004640.1:exon:2597CmaCh14G004640.1:exon:2597exon
CmaCh14G004640.1:exon:2598CmaCh14G004640.1:exon:2598exon
CmaCh14G004640.1:exon:2599CmaCh14G004640.1:exon:2599exon
CmaCh14G004640.1:exon:2600CmaCh14G004640.1:exon:2600exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh14G004640.1:cdsCmaCh14G004640.1:cdsCDS
CmaCh14G004640.1:cdsCmaCh14G004640.1:cds_2CDS
CmaCh14G004640.1:cdsCmaCh14G004640.1:cds_3CDS
CmaCh14G004640.1:cdsCmaCh14G004640.1:cds_4CDS
CmaCh14G004640.1:cdsCmaCh14G004640.1:cds_5CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh14G004640.1:three_prime_utrCmaCh14G004640.1:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh14G004640.1CmaCh14G004640.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006790 sulfur compound metabolic process
biological_process GO:0009150 purine ribonucleotide metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003824 catalytic activity
molecular_function GO:0047627 adenylylsulfatase activity