CmaCh14G001500.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G001500.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionKinesin-like protein
LocationCma_Chr14: 621922 .. 625618 (+)
Sequence length2247
RNA-Seq ExpressionCmaCh14G001500.1
SyntenyCmaCh14G001500.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAACGTCTTGATTTTCAAATTCACCTCCCTCGTCCCTCCCTTCGCTTGGAGACTCTGCCACTGCCGACGCGAACCATACTTCCAGTTCAATGGCTGCTGAAAATTTTGAACTTCTTGCACCTGCTACATCCTGTGCAATCTCCAAGGTCAGAGTCGTTGCTAGGGTTCGTCCATTTCTCTCCCAAGAAATTTCAGTCAAAAGTTCTAGGGTTCCAGTTTCATGCATTTCTGTCGTGGATCAAGGGCGTATCTCTCCCGAGGAGGTTACTGTTAATCTCAAAGATCCGGAAACCAGGTACTTTGTTTTTTATTTTTTCTCTGTATAGTGTTTTTCTGCCGTGGTAGAAGAATATTTTGGTTTGATTGATTATGGTTGGTGGATCTTCTGTAGTCGAAACGAGTGCTATCAGTTGGACTCATTTTTTGGCCAAGAAGATCACACGGTGAGGCAGATATTCGATAAAGAAGTAAATCCTTTGATTCCTGGACTCTTCCAAGGATGTAATGCTACTGTGTTTGCTTACGGGGCTACTGGCAGTGGAAAGTCCTACACTATGCAGGTTATCTTTAATTAGTGGGAGATTTGTCATCTGTTGCATTTAGCTTGAAATCAACAGAAGAATCATTAGCTTTCGTTCATAAAAGATTTAGCTTTCCTGAAATTGATATGAAATAATTCAACTACAGAAATGACTCGCACTCTGAATTGTGTCGATTTGGTTGAGGATGCAGGGTACTGAAGAAGAACCAGGTCTAATGCCACTAGCCATGTCTAGAGTTTTATCACTTTGTGAGGAAACTGGGTGTAGAGCAGAAATGTCGTACTATGAAGTCTACTTGGAAAGGTGTCATGATCTTCTGGAACCCAAAGCTAAAGAAATTGTAATTATGGATGATAAAGAAGGGCAAATTCATCTCAGAGGTTTATCTAAGGTTGCTGTAAATTCCATGTCCGAATTCCGGGAGACATTGGCTCTTGGAGTTCAAAGGCGGAAAGTTGCTGATACTGATCTTAACGATGTCTCAAGTAGAAGTCATGGGGTTCTTGTTATTACAGTTTCATCGCCCGTATGTGGTGATTCGGCGGCTTCTGTTACCGGAAAGCTCAACCTCATAGACTTGGCAGGTATGTTTCTTAAGCTTAATATAGAAACCTAGCCTTAACCATCAAATAAGATCTGTGCACTGCTCAAGAAGTTGTTCGTGTTGCTAATTTAGTTAATACTTGTGCATAACCATCCATCACAGGCAATGAAGATAACAGGAGGACTGGCAATGAAGGGATTCGCCTTCAAGAGAGCGCGAAAATCAACCAGTCGTTGTTCGCGCTTTCAAATGTGATATATGCATTAAACAAGAACCTTGCCCGAGTACCTTACAGAGAAAGCAAATTGACCCGCATACTGCAAGATTCTCTCGGTGGAACAAGCCGTGCCTTAATGATTGCTTGCCTGGTAAATTGATGCAGGTTAAACATCTTTTGTAATCTAAGAATTGCACTTGCTCAAATTTCCTTCTGATTTGATAACAGAATCCAGGAGCATATCAGGAGTCTGTCCATACAGTCAGCTTGGCAGCTAGATCGCGTCAAATTTCGAATTTTGTTGTTCCGATCCATAAACAGGGGACTCCACAAGCTAAAGTTGACATGGAATCGAAGCTGCGAGCTTGGCTTGAATCCAAAGGCAAGACCAAAAGTGCGCAAAGAATCGGAGGGATTGGTTCTCCATTTTCTAGCAAAACTCCTTGTTCTACTGCATCAAAAGTGAGAAAACCTCTCTTCAATAGTTCTGCCAAAGTTAAGAAAACGGCCAACCAGAGTGACTGTAAACGAGAAAAAGAAAGGTCTGGCAATCATTTGTTGCTTTATCAACTTTTAGTACCTTCTACTAATTCTTTTAACAATACTGGATTGATGATCTTCAGGACTGTAGCCGTAGCTTTCGGAAACTTGGTTGAAAATAAAGATATAGCTAATTCTATCGCAACCAAAGATCATGGTATTTTCTATCACAGCTGCCTCGTTCATTCTGCCACATTGTGTTTGGATATGATTGAATCTTCATGTTTATATGCAGTAGAAAAGGTGAAATTCAATAGTCCAAGAAAGGCTCTATCACCAATCCACAACACTAATGGAATCCAAAAGGCTGTGCTGGAAATCTCGTTTACAGATCAATTACCAGCAAGGACTCCAAAAACACCATTCCCAGCAAATGAGGAATTCAAATCTTTTGGGACCCCATTAGATAAGTTTGATGCCCAAGTATCAACCCTCAAGGTACTCAAAAAGCCTTTTTAAAATTTCATTATCTCTTTATGTCTCGATGCTGTTTGGATTTCAATAATTCTGGTTTTTCTTGTCATTCTAACCAATCAGAGTTGCGTTGCACAAGAATATGTGGATTTCTTGAATACAGCAAGCAGGTAGGTCCATAATCATATACAATATTTAGCATTTTGTGCACAAGAATATGTGGATTTCTTGTTCCAGTACCCAACTTTTCAATTTGAAATAGTGAAAGTTTAATCAATTATTAATTGGCTTTCTTTGTTTTGCAGAGAGGAACTAGTAGAATTGAAGGTATTTTCTAGACTTCGACCAGGAAAAACATTAACATCTTCCCTTTAAGTTTCCTCATTCTCTTTTCCCTTTCAATTCCCAGGGCATTGGAGTGAAAATGGCAGAGTACATTCTGGATCTCAGAGAAACTAGTCCATTGAAATCGGTAAGACGATAACCACGAGATATATGTTTGATGTATTTCTCTCATATGTTCAATGGTGCGTGGTGGTTTCATCCAAAATTTTCTTATAGTGGACATTCATGGTTACCAAGTCCATCTTCTCATGTCATATGCACTTGGGACCTAACCTTATCCCTTTTCTCTCCTAGTCCAATCTGTGTTCTCTAAATTCTTCATTTTGGTGTCTAAAAGTTTGCCAAGCACGAGAGACCTCTATCATTCGCAAATACAATCCAACCCCACACACACATAGTTTTGTCATTTCTGATAGTCACTCAACATGTAAAAACATCGCATGTAAACTTACAAAAACACGTCGATAAAGCAACACTATCCAACTTCTTTCCTGAATATAATGAACTTTTTCTTCCCCTTGCTGTGTTTACATAGCTTGCTGACCTGAAGAAACTAGGACTCTCATCCAAGCAGGTAATGCTTCAACAAACACAATAACTTACCAAATTTACCATTTTGGTTCACTCCTGGAAAAATCAGGCTCCCCTAAATATGGATTCTTTTTCTTCTGCAGATCCATAACTTATTCAGCAAAGCTGCAATGGGGATATTTGGTAGAATAGAAGATTTGGCTGCTGTCCCTTGCCCTGAAACTTCATTATCCAACCAATAATGAAGGTAGAAGAAGATATAGTGATGTATTGGGAAGTGTGCCATAGCAACATCCTCCAAGTTGCTGTATGTTAACATATTGTAACAGTAGAGAAGAGAACTGTCCGACTAAAGATTTTGCGTGGTCGGCGTGATGGGCTTCCTTCATGTTCTCTGTAAGCCCTATCTGTATTATTCATTAACCAAATTATCTATTTGTATTACTATGAACTTGTGGCTATTGTAATCTAAATATGATGATCCAGAGTGATGCTGATTTGTCGCTATTTTTATGGCTTATATGGAACCAGAGCAGATATTTAATTTAAACAAAATAAAGTAT

mRNA sequence

CCAACGTCTTGATTTTCAAATTCACCTCCCTCGTCCCTCCCTTCGCTTGGAGACTCTGCCACTGCCGACGCGAACCATACTTCCAGTTCAATGGCTGCTGAAAATTTTGAACTTCTTGCACCTGCTACATCCTGTGCAATCTCCAAGGTCAGAGTCGTTGCTAGGGTTCGTCCATTTCTCTCCCAAGAAATTTCAGTCAAAAGTTCTAGGGTTCCAGTTTCATGCATTTCTGTCGTGGATCAAGGGCGTATCTCTCCCGAGGAGGTTACTGTTAATCTCAAAGATCCGGAAACCAGTCGAAACGAGTGCTATCAGTTGGACTCATTTTTTGGCCAAGAAGATCACACGGTGAGGCAGATATTCGATAAAGAAGTAAATCCTTTGATTCCTGGACTCTTCCAAGGATGTAATGCTACTGTGTTTGCTTACGGGGCTACTGGCAGTGGAAAGTCCTACACTATGCAGGGTACTGAAGAAGAACCAGGTCTAATGCCACTAGCCATGTCTAGAGTTTTATCACTTTGTGAGGAAACTGGGTGTAGAGCAGAAATGTCGTACTATGAAGTCTACTTGGAAAGGTGTCATGATCTTCTGGAACCCAAAGCTAAAGAAATTGTAATTATGGATGATAAAGAAGGGCAAATTCATCTCAGAGGTTTATCTAAGGTTGCTGTAAATTCCATGTCCGAATTCCGGGAGACATTGGCTCTTGGAGTTCAAAGGCGGAAAGTTGCTGATACTGATCTTAACGATGTCTCAAGTAGAAGTCATGGGGTTCTTGTTATTACAGTTTCATCGCCCGTATGTGGTGATTCGGCGGCTTCTGTTACCGGAAAGCTCAACCTCATAGACTTGGCAGGCAATGAAGATAACAGGAGGACTGGCAATGAAGGGATTCGCCTTCAAGAGAGCGCGAAAATCAACCAGTCGTTGTTCGCGCTTTCAAATGTGATATATGCATTAAACAAGAACCTTGCCCGAGTACCTTACAGAGAAAGCAAATTGACCCGCATACTGCAAGATTCTCTCGGTGGAACAAGCCGTGCCTTAATGATTGCTTGCCTGAATCCAGGAGCATATCAGGAGTCTGTCCATACAGTCAGCTTGGCAGCTAGATCGCGTCAAATTTCGAATTTTGTTGTTCCGATCCATAAACAGGGGACTCCACAAGCTAAAGTTGACATGGAATCGAAGCTGCGAGCTTGGCTTGAATCCAAAGGCAAGACCAAAAGTGCGCAAAGAATCGGAGGGATTGGTTCTCCATTTTCTAGCAAAACTCCTTGTTCTACTGCATCAAAAGTGAGAAAACCTCTCTTCAATAGTTCTGCCAAAGTTAAGAAAACGGCCAACCAGAGTGACTGTAAACGAGAAAAAGAAAGGACTGTAGCCGTAGCTTTCGGAAACTTGGTTGAAAATAAAGATATAGCTAATTCTATCGCAACCAAAGATCATGTAGAAAAGGTGAAATTCAATAGTCCAAGAAAGGCTCTATCACCAATCCACAACACTAATGGAATCCAAAAGGCTGTGCTGGAAATCTCGTTTACAGATCAATTACCAGCAAGGACTCCAAAAACACCATTCCCAGCAAATGAGGAATTCAAATCTTTTGGGACCCCATTAGATAAGTTTGATGCCCAAGTATCAACCCTCAAGAGTTGCGTTGCACAAGAATATGTGGATTTCTTGAATACAGCAAGCAGAGAGGAACTAGTAGAATTGAAGGGCATTGGAGTGAAAATGGCAGAGTACATTCTGGATCTCAGAGAAACTAGTCCATTGAAATCGCTTGCTGACCTGAAGAAACTAGGACTCTCATCCAAGCAGATCCATAACTTATTCAGCAAAGCTGCAATGGGGATATTTGGTAGAATAGAAGATTTGGCTGCTGTCCCTTGCCCTGAAACTTCATTATCCAACCAATAATGAAGGTAGAAGAAGATATAGTGATGTATTGGGAAGTGTGCCATAGCAACATCCTCCAAGTTGCTGTATGTTAACATATTGTAACAGTAGAGAAGAGAACTGTCCGACTAAAGATTTTGCGTGGTCGGCGTGATGGGCTTCCTTCATGTTCTCTGTAAGCCCTATCTGTATTATTCATTAACCAAATTATCTATTTGTATTACTATGAACTTGTGGCTATTGTAATCTAAATATGATGATCCAGAGTGATGCTGATTTGTCGCTATTTTTATGGCTTATATGGAACCAGAGCAGATATTTAATTTAAACAAAATAAAGTAT

Coding sequence (CDS)

ATGGCTGCTGAAAATTTTGAACTTCTTGCACCTGCTACATCCTGTGCAATCTCCAAGGTCAGAGTCGTTGCTAGGGTTCGTCCATTTCTCTCCCAAGAAATTTCAGTCAAAAGTTCTAGGGTTCCAGTTTCATGCATTTCTGTCGTGGATCAAGGGCGTATCTCTCCCGAGGAGGTTACTGTTAATCTCAAAGATCCGGAAACCAGTCGAAACGAGTGCTATCAGTTGGACTCATTTTTTGGCCAAGAAGATCACACGGTGAGGCAGATATTCGATAAAGAAGTAAATCCTTTGATTCCTGGACTCTTCCAAGGATGTAATGCTACTGTGTTTGCTTACGGGGCTACTGGCAGTGGAAAGTCCTACACTATGCAGGGTACTGAAGAAGAACCAGGTCTAATGCCACTAGCCATGTCTAGAGTTTTATCACTTTGTGAGGAAACTGGGTGTAGAGCAGAAATGTCGTACTATGAAGTCTACTTGGAAAGGTGTCATGATCTTCTGGAACCCAAAGCTAAAGAAATTGTAATTATGGATGATAAAGAAGGGCAAATTCATCTCAGAGGTTTATCTAAGGTTGCTGTAAATTCCATGTCCGAATTCCGGGAGACATTGGCTCTTGGAGTTCAAAGGCGGAAAGTTGCTGATACTGATCTTAACGATGTCTCAAGTAGAAGTCATGGGGTTCTTGTTATTACAGTTTCATCGCCCGTATGTGGTGATTCGGCGGCTTCTGTTACCGGAAAGCTCAACCTCATAGACTTGGCAGGCAATGAAGATAACAGGAGGACTGGCAATGAAGGGATTCGCCTTCAAGAGAGCGCGAAAATCAACCAGTCGTTGTTCGCGCTTTCAAATGTGATATATGCATTAAACAAGAACCTTGCCCGAGTACCTTACAGAGAAAGCAAATTGACCCGCATACTGCAAGATTCTCTCGGTGGAACAAGCCGTGCCTTAATGATTGCTTGCCTGAATCCAGGAGCATATCAGGAGTCTGTCCATACAGTCAGCTTGGCAGCTAGATCGCGTCAAATTTCGAATTTTGTTGTTCCGATCCATAAACAGGGGACTCCACAAGCTAAAGTTGACATGGAATCGAAGCTGCGAGCTTGGCTTGAATCCAAAGGCAAGACCAAAAGTGCGCAAAGAATCGGAGGGATTGGTTCTCCATTTTCTAGCAAAACTCCTTGTTCTACTGCATCAAAAGTGAGAAAACCTCTCTTCAATAGTTCTGCCAAAGTTAAGAAAACGGCCAACCAGAGTGACTGTAAACGAGAAAAAGAAAGGACTGTAGCCGTAGCTTTCGGAAACTTGGTTGAAAATAAAGATATAGCTAATTCTATCGCAACCAAAGATCATGTAGAAAAGGTGAAATTCAATAGTCCAAGAAAGGCTCTATCACCAATCCACAACACTAATGGAATCCAAAAGGCTGTGCTGGAAATCTCGTTTACAGATCAATTACCAGCAAGGACTCCAAAAACACCATTCCCAGCAAATGAGGAATTCAAATCTTTTGGGACCCCATTAGATAAGTTTGATGCCCAAGTATCAACCCTCAAGAGTTGCGTTGCACAAGAATATGTGGATTTCTTGAATACAGCAAGCAGAGAGGAACTAGTAGAATTGAAGGGCATTGGAGTGAAAATGGCAGAGTACATTCTGGATCTCAGAGAAACTAGTCCATTGAAATCGCTTGCTGACCTGAAGAAACTAGGACTCTCATCCAAGCAGATCCATAACTTATTCAGCAAAGCTGCAATGGGGATATTTGGTAGAATAGAAGATTTGGCTGCTGTCCCTTGCCCTGAAACTTCATTATCCAACCAATAA

Protein sequence

MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQAKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTANQSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAVLEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPCPETSLSNQ
Homology
BLAST of CmaCh14G001500.1 vs. ExPASy Swiss-Prot
Match: Q5E913 (Kinesin-like protein KIN-10B OS=Arabidopsis thaliana OX=3702 GN=KIN10B PE=2 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 7.5e-168
Identity = 349/624 (55.93%), Postives = 421/624 (67.47%), Query Frame = 0

Query: 14  SCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNEC 73
           S +IS VRVV RVRPFL +EIS +S     SC+SV+        EV V LKDP++ RNE 
Sbjct: 15  SSSISNVRVVLRVRPFLPREISDESCD-GRSCVSVIGGDGGDTSEVAVYLKDPDSCRNES 74

Query: 74  YQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQGTEEEPGL 133
           YQLD+F+G+ED  V+ IFD+EV+PLIPG+F G NATV AYGATGSGK++TMQG +E PGL
Sbjct: 75  YQLDAFYGREDDNVKHIFDREVSPLIPGIFHGFNATVLAYGATGSGKTFTMQGIDELPGL 134

Query: 134 MPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLRGLSKV 193
           MPL MS +LS+CE+T  RAE+SYYEVY++RC DLLE K  EI + DDK+GQ+HL+GLS V
Sbjct: 135 MPLTMSTILSMCEKTRSRAEISYYEVYMDRCWDLLEVKDNEIAVWDDKDGQVHLKGLSSV 194

Query: 194 AVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTGKLNLI 253
            V SMSEF+E    GVQRRKVA T LNDVSSRSHGVLVI+V+      S   VTGK+NLI
Sbjct: 195 PVKSMSEFQEAYLCGVQRRKVAHTGLNDVSSRSHGVLVISVT------SQGLVTGKINLI 254

Query: 254 DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRILQDSL 313
           DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNV+YALN NL RVPYRE+KLTRILQDSL
Sbjct: 255 DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVVYALNNNLPRVPYRETKLTRILQDSL 314

Query: 314 GGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQAKVDMESKLRAWL 373
           GGTSRALM+ACLNPG YQES+ TVSLAARSR ISN V    K  TP+ K+DME+KL+AWL
Sbjct: 315 GGTSRALMVACLNPGEYQESLRTVSLAARSRHISNNVSLNPKVETPKVKIDMEAKLQAWL 374

Query: 374 ESKGKTKSAQRIGGIGSPFSSKTPCS-TASKVRKPLFNSSAKVKKTANQSDCKREKERTV 433
           ESKGK KSA R+  I SP       S + S V+K L + SA  +        +R+   T 
Sbjct: 375 ESKGKMKSAHRMMAIRSPLMGTNQTSISQSSVKKLLCHRSAIAESAKLAGTGQRDAFVTA 434

Query: 434 AVAFG--------------NLV--------------ENKDIANSIATKDH---VEK--VK 493
              FG              NL               E   + +  + +D+   VEK   +
Sbjct: 435 RNLFGVETLAASHLWEPIRNLQLASPTKEDERDTSGEENLLVSEASLRDNTLDVEKKYTE 494

Query: 494 FNSPRKALSPIHNTNGIQKAVLEISFTDQLPARTPKTPF-PANEEFKSFGTPLDKFDAQV 553
            +  R+ALSPI +      A     F   L   TPKTPF   N E         KF+A  
Sbjct: 495 LSPLREALSPIDSNAKPNSAHGSSPF---LKPMTPKTPFLSTNPEIMQMEGTCQKFNAWS 554

Query: 554 STLKSCVAQEYVDFLNTASREELVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSK 603
           + LK+ + +EY+ FLNTA+REEL ELKGIG KMAEYI++LRETSPLKSL DL+K+G +S+
Sbjct: 555 NNLKTSLIKEYIHFLNTANREELRELKGIGQKMAEYIIELRETSPLKSLTDLEKIGFTSR 614

BLAST of CmaCh14G001500.1 vs. ExPASy Swiss-Prot
Match: Q6L512 (Kinesin-like protein KIN-10C OS=Oryza sativa subsp. japonica OX=39947 GN=KIN10C PE=2 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 5.0e-156
Identity = 335/600 (55.83%), Postives = 407/600 (67.83%), Query Frame = 0

Query: 9   LAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPET 68
           +A A +     VRVV RVRP L  E    S+  P  C+ ++  G     EVTV LKD  T
Sbjct: 1   MATAAATQSQPVRVVLRVRPHLPSE--ANSAEAP--CVGLL--GSHPGGEVTVQLKDQYT 60

Query: 69  SRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQGTE 128
           SRNECY+LD+FFGQE   V +IFD+EV+ +IPG+F+G NATVFAYGATGSGK+YTMQGTE
Sbjct: 61  SRNECYKLDAFFGQESR-VCEIFDQEVSAVIPGIFEGTNATVFAYGATGSGKTYTMQGTE 120

Query: 129 EEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLR 188
           + PGL+PLA+S VL+LC  T C  E+SYYEVY+ERC+DLLEPKA+EI+++DDK+G + L+
Sbjct: 121 DLPGLIPLAVSTVLALCTGTWCSVEISYYEVYMERCYDLLEPKAREIMVLDDKDGNLQLK 180

Query: 189 GLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTG 248
           GL+ V V S+ EF E  ++GVQRRKVA T LNDVSSRSH VL I +++ V       V G
Sbjct: 181 GLAWVPVRSLEEFHEIYSIGVQRRKVAHTGLNDVSSRSHAVLSIRITTDV-------VKG 240

Query: 249 KLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRI 308
           KLNLIDLAGNEDNRRT NEGIRLQESAKINQSLFALSNVI ALNK   R+PYRESKLTRI
Sbjct: 241 KLNLIDLAGNEDNRRTCNEGIRLQESAKINQSLFALSNVISALNKKEPRIPYRESKLTRI 300

Query: 309 LQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQAKVDMESK 368
           LQDSLGG S A+MIACLNP  YQE+VHTVSLAARSR ++N +    KQ TP+ KVDME+K
Sbjct: 301 LQDSLGGNSHAVMIACLNPVEYQEAVHTVSLAARSRHVTNHMSSASKQETPKDKVDMEAK 360

Query: 369 LRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVR----------KPLFNSSAKVKKT 428
           LRAWLESKGKTKS QR+ G+ SP + KTP S + K +          K +     K+KK 
Sbjct: 361 LRAWLESKGKTKSIQRMDGLLSPNAIKTPLSMSHKKQSASGRVSGRGKAMDQDGGKIKKV 420

Query: 429 ANQSDCKREKERTVAVAFGNLVE-NKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQ 488
              S  +   E        ++V   K +     T    +K   +S RKALSPI +    Q
Sbjct: 421 LFDSAARIPAENFHREGTQDIVNTTKKVVLPSLTPCKEDKTG-SSLRKALSPISSNMDPQ 480

Query: 489 K--AVLEISFTDQLPARTPKTPFPANEEFKSFG-TPLDKFDAQVSTLKSCVAQEYVDFLN 548
           K     + +    L  RTP      N   K  G TPLDKF A  S LK  + Q+Y+DFLN
Sbjct: 481 KQRTADDSNCLMLLELRTPMG--SCNIVGKVTGATPLDKFIALGSNLKESLIQQYLDFLN 540

Query: 549 TASREELVELKGIGVKMAEYILDLRETS--PLKSLADLKKLGLSSKQIHNLFSKAAMGIF 593
            A++EEL +LKGIG + AEYIL+LRE S  P KSL+DL  +GLSSKQI ++  K A GIF
Sbjct: 541 VANKEELQKLKGIGERRAEYILELREDSPRPFKSLSDLGNIGLSSKQIQDILCKTATGIF 583

BLAST of CmaCh14G001500.1 vs. ExPASy Swiss-Prot
Match: Q6P3R1 (Kinesin-like protein KIF22 OS=Xenopus tropicalis OX=8364 GN=kif22 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.6e-67
Identity = 213/641 (33.23%), Postives = 319/641 (49.77%), Query Frame = 0

Query: 18  SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNECYQLD 77
           ++VRV  R+RP++ +E      + P +C+  +D   +   E+       ET +   YQ D
Sbjct: 17  ARVRVAVRLRPYMEKE----DEKAPAACVRGLDSQSL---EIVNWRNQLETMQ---YQFD 76

Query: 78  SFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQGTEEEPGLMPLA 137
           +F+G +  T R+I+   V  ++P L  G NA+VFAYG TG+GK++TM G   +PG++P A
Sbjct: 77  AFYG-DSATQREIYMGSVCHILPHLLIGQNASVFAYGPTGAGKTHTMLGNPSQPGVIPRA 136

Query: 138 MSRVLSLCEETGCRAE---------MSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLR 197
           +  +L +        E         MSY E+Y E+  DLLEPK K++ I +DK+  I + 
Sbjct: 137 VRDLLQMTRTAAGGPENENWTYTITMSYVEIYQEKVMDLLEPKNKDLPIREDKDHNILIP 196

Query: 198 GLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTG 257
           G+++  +NS  +F E      Q R VA T LND SSRSH VL+I V           +TG
Sbjct: 197 GVTQKTINSFGDFDEHFIPASQNRTVASTKLNDRSSRSHAVLLIKVQKSQQVSPFRQLTG 256

Query: 258 KLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRI 317
           KL LIDLAG+EDNRRTGN+GIRL+ES  IN SLF LS V+ ALN+ L R+PYR+SKLTR+
Sbjct: 257 KLYLIDLAGSEDNRRTGNQGIRLKESGAINSSLFTLSKVVDALNQGLPRIPYRDSKLTRL 316

Query: 318 LQDSLGGTSRALMIACLNPGA--YQESVHTVSLAARSRQISN-----------FVVPIHK 377
           LQDSLGGT+ ++MIA + P    Y +++  ++ AA+S+QI N             +P  K
Sbjct: 317 LQDSLGGTAHSVMIANIAPEQKYYFDTLTALNFAAKSKQIINKPFSQETTQSIAALPAMK 376

Query: 378 QGTPQAKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTAS----------KVR 437
           +   +A+    S+ R     K KT S +         +SK   + A+          K+ 
Sbjct: 377 RPREEAETAAGSRQR----KKSKTDSTESSPNTSMDAASKRKLNLAALDPAVVERLLKLD 436

Query: 438 KPLFNSSAKVKKTANQSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRK 497
           K L  +   +K+    S  KRE+   +     + +E + +      K+  +K      R 
Sbjct: 437 KIL--TEKGMKEAQLLSTPKRERMALLKKWEESQMEIERLKEK--QKELEQKAIEAEARL 496

Query: 498 ALSPIHNTNGIQKAVLEISFTDQLPARTPKT----------PFPANEEFKSF---GTPLD 557
             S   + N    +V E +F   L  R   T          P   N + +S    G P+ 
Sbjct: 497 EKSTNSDCNLSDSSVSECTFRAPLRGRNTSTAKAKKVLRVLPMQGNSQLQSTIEEGIPV- 556

Query: 558 KFDAQVSTLKSCVAQE--------------------YVDFLNTASREELVELKGIGVKMA 592
            F+ +     SC  +E                     +  LNT S +EL  L+ IG K A
Sbjct: 557 -FEKKKKKPVSCDGRENQPTWEVNVRTDLLESGRERILKLLNTGSVKELKSLQKIGDKKA 616

BLAST of CmaCh14G001500.1 vs. ExPASy Swiss-Prot
Match: Q3V300 (Kinesin-like protein KIF22 OS=Mus musculus OX=10090 GN=Kif22 PE=2 SV=2)

HSP 1 Score: 256.1 bits (653), Expect = 9.7e-67
Identity = 205/635 (32.28%), Postives = 320/635 (50.39%), Query Frame = 0

Query: 17  ISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNECYQL 76
           +++VRV  R+RPF+  E   K     + C+  +D   +   EV    K  ET +   YQ 
Sbjct: 36  LARVRVAVRLRPFMDGETEAKE----LPCVRAIDSCSL---EVANWKKYQETLK---YQF 95

Query: 77  DSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQGTEEEPGLMPL 136
           D+F+G E  T ++++   V P++  L +G NA+V AYG TG+GK++TM G+ E+PG++P 
Sbjct: 96  DAFYG-EKSTQQEVYVGSVQPILRHLLEGQNASVLAYGPTGAGKTHTMLGSPEQPGVIPR 155

Query: 137 AMSRVLSLCEETGCRAE-------MSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLRG 196
           A+  +L L  E             MSY E+Y E+  DLL+P + ++VI +D  G I + G
Sbjct: 156 ALMDLLQLAREESAEGRPWDVSVAMSYLEIYQEKVLDLLDPASGDLVIREDCRGNILIPG 215

Query: 197 LSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTGK 256
           L++  + S S+F +      + R V  T LN  SSRSH VL++ V             GK
Sbjct: 216 LTQKPITSFSDFEQHFLPASRNRAVGATRLNQRSSRSHAVLLVKVDQRERLTPFRQREGK 275

Query: 257 LNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRIL 316
           L LIDLAG+EDNRRTGN+GIRL+ES  IN SLF L  V+ ALN+ L R+PYR+SKLTR+L
Sbjct: 276 LYLIDLAGSEDNRRTGNQGIRLKESGAINTSLFVLGKVVDALNQGLPRIPYRDSKLTRLL 335

Query: 317 QDSLGGTSRALMIACLNPGA--YQESVHTVSLAARSRQISNFVVPIHKQGTPQAKVDMES 376
           QDSLGG++ +++IA + P    YQ+++  ++  ARS+++ N     ++   P A   ++ 
Sbjct: 336 QDSLGGSAHSILIANIAPERRFYQDTISALNFTARSKEVINRPF-TNESLQPHALAPVKL 395

Query: 377 KLRAWL-ESKGKTKSAQRIGGIGSPFSSKTPCSTASKVR--------------------- 436
             +  L  S+ K          GSP S+  P S + K+                      
Sbjct: 396 SQKELLGPSEAKKAKGPEEESTGSPESTAAPASASQKLSLLQKLSNMDPAMLENLLSMER 455

Query: 437 ---------KPLFNSSAKVKKTANQSDCKREKERTVAVAFGNLVENKDIANSIATKDHVE 496
                     PL N+  + +        K  +E+ + +    + + +  A  +A +    
Sbjct: 456 LLGSQGSQGTPLLNTPKRERMVL----MKTVEEKNLEIERLKMKQKELEAKVLAQEAPDP 515

Query: 497 KVKFNSPRKALSPIHNTNG-----IQKAVL----------EISFTDQLPARTPKTPFPAN 556
           + K N+P   L P  + +G     ++KAV+          E S   QL  + PK     +
Sbjct: 516 REKENTP-TILQPPASYSGTVAKPLKKAVVMPLQRIQKQRESSNQIQLLKKGPKRKLEPS 575

Query: 557 EEFKSFGTPLDKFDAQVS-TLKSCVAQEYVDFLNTASREELVELKGIGVKMAEYILDLRE 589
            E ++     D ++ Q+S  L +   ++ +D LN  S  EL  L+ IG K A+ I+  RE
Sbjct: 576 PESEAVEKDEDYWEVQISPELLAHGRKKLLDLLNEGSARELRSLQRIGQKKAQLIVGWRE 635

BLAST of CmaCh14G001500.1 vs. ExPASy Swiss-Prot
Match: Q9I869 (Kinesin-like protein KIF22-A OS=Xenopus laevis OX=8355 GN=kif22-a PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 7.7e-64
Identity = 206/635 (32.44%), Postives = 319/635 (50.24%), Query Frame = 0

Query: 13  TSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNE 72
           +SCA  +VRV  R+RP++ ++   K++ V   C+  +D   +   E+       ET +  
Sbjct: 27  SSCA--RVRVAVRLRPYMDEKDEAKATTV---CVRGLDSQSL---EIVNWRNQLETMQ-- 86

Query: 73  CYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQGTEEEPG 132
            YQ D+F+G +  + R+I+   V  ++P L  G NA+VFAYG TG+GK++TM G   +PG
Sbjct: 87  -YQFDAFYG-DSASQREIYMGSVCHILPHLLIGQNASVFAYGPTGAGKTHTMLGNPNQPG 146

Query: 133 LMPLAMSRVLSLCEETGCRAE---------MSYYEVYLERCHDLLEPKAKEIVIMDDKEG 192
           ++P A+  +L +        E         MSY E+Y E+  DLLEPK K++ I +DK+ 
Sbjct: 147 VIPRAVRDLLQMSRTAASAPENENWTYTINMSYVEIYQEKVMDLLEPKNKDLPIREDKDH 206

Query: 193 QIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSA 252
            I + G+++  +NS ++F E      Q R VA T LND SSRSH VL+I V         
Sbjct: 207 NILIPGVTQKMINSFADFDEHFIPASQNRTVASTKLNDRSSRSHAVLLIKVQKSQQVVPF 266

Query: 253 ASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRES 312
             +TGKL LIDLAG+EDNRRTGN+GIRL+ES  IN SLF LS V+ ALN+ L R+PYR+S
Sbjct: 267 RQLTGKLYLIDLAGSEDNRRTGNQGIRLKESGAINSSLFTLSKVVDALNQGLPRIPYRDS 326

Query: 313 KLTRILQDSLGGTSRALMIACLNPGA--YQESVHTVSLAARSRQISNFVVPIHKQGTPQA 372
           KLTR+LQDSLGG++ ++MI  + P    Y +++  ++ AA+S+QI N   P  ++ T   
Sbjct: 327 KLTRLLQDSLGGSAHSVMITNIAPEQTYYFDTLTALNFAAKSKQIIN--KPFSQETT--- 386

Query: 373 KVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSA-------- 432
           +  ++  ++   E  G    +Q+     +  +  +P S+     K   N +         
Sbjct: 387 QTVVQPAMKRPREETGHIAGSQKRKKSKNDSTESSPNSSMDTAGKQKLNLATLDPAVVER 446

Query: 433 --KVKKTANQSDCKREK-----ERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKA 492
             K+ K   +   K+ +     +R          E++     +  K    + K       
Sbjct: 447 LLKLDKILTEKGKKKAQLLSTPKRERMALLKKWEESQMEIERLKEKQKELEQKAMEAEAR 506

Query: 493 LSPIHNTNGIQKAVLEISFTDQLPARTPKT----------PFPANEEFKSF---GTPL-- 552
           L   +N++    +V E +F   L  R   T          P   N + +S    G P+  
Sbjct: 507 LEKSNNSDLSDSSVSENTFRAPLRGRNTSTAKVKKVLRVLPMQGNSQLQSTVEEGIPVFE 566

Query: 553 -DKFDAQV----------------STLKSCVAQEYVDFLNTASREELVELKGIGVKMAEY 588
             K   QV                + L     +  +  LNT S +EL  L+ IG K A+ 
Sbjct: 567 KKKKKKQVTCEGLENQPTWEMNMRTDLLESGKERILKLLNTGSVKELKSLQRIGDKKAKL 626

BLAST of CmaCh14G001500.1 vs. ExPASy TrEMBL
Match: A0A6J1J7B8 (Kinesin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482032 PE=3 SV=1)

HSP 1 Score: 1183.7 bits (3061), Expect = 0.0e+00
Identity = 611/611 (100.00%), Postives = 611/611 (100.00%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT
Sbjct: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480
           QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480

Query: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 612
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 611

BLAST of CmaCh14G001500.1 vs. ExPASy TrEMBL
Match: A0A6J1J5U2 (Kinesin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482032 PE=3 SV=1)

HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 610/611 (99.84%), Postives = 610/611 (99.84%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT
Sbjct: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480
           QSDCKREKERTVAVAFGNLVENKDIANSIATKDH EKVKFNSPRKALSPIHNTNGIQKAV
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDH-EKVKFNSPRKALSPIHNTNGIQKAV 480

Query: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 612
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 610

BLAST of CmaCh14G001500.1 vs. ExPASy TrEMBL
Match: A0A6J1F8V4 (Kinesin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441883 PE=3 SV=1)

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 604/611 (98.85%), Postives = 606/611 (99.18%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCA+SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQG ISPEEVT
Sbjct: 1   MAAENFELLAPATSCAVSKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGHISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLF GCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480
           QSDCKREKERTVAVAFGNLVENKDIANSI TKDHVEKVKFNSPRKALSPIHNTNGIQK V
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSITTKDHVEKVKFNSPRKALSPIHNTNGIQKGV 480

Query: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LE+SF DQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LELSFADQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 612
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 611

BLAST of CmaCh14G001500.1 vs. ExPASy TrEMBL
Match: A0A6J1J264 (Kinesin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482032 PE=3 SV=1)

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 608/611 (99.51%), Postives = 608/611 (99.51%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT
Sbjct: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480
           QSDCKREKERTVAVAFGNLVENKDIANSIATK   EKVKFNSPRKALSPIHNTNGIQKAV
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATK---EKVKFNSPRKALSPIHNTNGIQKAV 480

Query: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 612
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 608

BLAST of CmaCh14G001500.1 vs. ExPASy TrEMBL
Match: A0A6J1F2Y6 (Kinesin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441883 PE=3 SV=1)

HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 603/611 (98.69%), Postives = 605/611 (99.02%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCA+SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQG ISPEEVT
Sbjct: 1   MAAENFELLAPATSCAVSKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGHISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLF GCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480
           QSDCKREKERTVAVAFGNLVENKDIANSI TKDH EKVKFNSPRKALSPIHNTNGIQK V
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSITTKDH-EKVKFNSPRKALSPIHNTNGIQKGV 480

Query: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LE+SF DQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LELSFADQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 612
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 610

BLAST of CmaCh14G001500.1 vs. NCBI nr
Match: XP_022983424.1 (kinesin-like protein KIN-10B isoform X1 [Cucurbita maxima])

HSP 1 Score: 1183.7 bits (3061), Expect = 0.0e+00
Identity = 611/611 (100.00%), Postives = 611/611 (100.00%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT
Sbjct: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480
           QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480

Query: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 612
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 611

BLAST of CmaCh14G001500.1 vs. NCBI nr
Match: XP_022983425.1 (kinesin-like protein KIN-10B isoform X2 [Cucurbita maxima])

HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 610/611 (99.84%), Postives = 610/611 (99.84%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT
Sbjct: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480
           QSDCKREKERTVAVAFGNLVENKDIANSIATKDH EKVKFNSPRKALSPIHNTNGIQKAV
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDH-EKVKFNSPRKALSPIHNTNGIQKAV 480

Query: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 612
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 610

BLAST of CmaCh14G001500.1 vs. NCBI nr
Match: XP_022934835.1 (kinesin-like protein KIN-10B isoform X1 [Cucurbita moschata])

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 604/611 (98.85%), Postives = 606/611 (99.18%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCA+SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQG ISPEEVT
Sbjct: 1   MAAENFELLAPATSCAVSKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGHISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLF GCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480
           QSDCKREKERTVAVAFGNLVENKDIANSI TKDHVEKVKFNSPRKALSPIHNTNGIQK V
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSITTKDHVEKVKFNSPRKALSPIHNTNGIQKGV 480

Query: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LE+SF DQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LELSFADQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 612
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 611

BLAST of CmaCh14G001500.1 vs. NCBI nr
Match: XP_022983426.1 (kinesin-like protein KIN-10B isoform X3 [Cucurbita maxima])

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 608/611 (99.51%), Postives = 608/611 (99.51%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT
Sbjct: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480
           QSDCKREKERTVAVAFGNLVENKDIANSIATK   EKVKFNSPRKALSPIHNTNGIQKAV
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATK---EKVKFNSPRKALSPIHNTNGIQKAV 480

Query: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 612
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 608

BLAST of CmaCh14G001500.1 vs. NCBI nr
Match: XP_022934836.1 (kinesin-like protein KIN-10B isoform X2 [Cucurbita moschata])

HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 603/611 (98.69%), Postives = 605/611 (99.02%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCA+SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQG ISPEEVT
Sbjct: 1   MAAENFELLAPATSCAVSKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGHISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLF GCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHVEKVKFNSPRKALSPIHNTNGIQKAV 480
           QSDCKREKERTVAVAFGNLVENKDIANSI TKDH EKVKFNSPRKALSPIHNTNGIQK V
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSITTKDH-EKVKFNSPRKALSPIHNTNGIQKGV 480

Query: 481 LEISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LE+SF DQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LELSFADQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 612
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 610

BLAST of CmaCh14G001500.1 vs. TAIR 10
Match: AT5G02370.1 (ATP binding microtubule motor family protein )

HSP 1 Score: 592.0 bits (1525), Expect = 5.3e-169
Identity = 349/624 (55.93%), Postives = 421/624 (67.47%), Query Frame = 0

Query: 14  SCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNEC 73
           S +IS VRVV RVRPFL +EIS +S     SC+SV+        EV V LKDP++ RNE 
Sbjct: 15  SSSISNVRVVLRVRPFLPREISDESCD-GRSCVSVIGGDGGDTSEVAVYLKDPDSCRNES 74

Query: 74  YQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQGTEEEPGL 133
           YQLD+F+G+ED  V+ IFD+EV+PLIPG+F G NATV AYGATGSGK++TMQG +E PGL
Sbjct: 75  YQLDAFYGREDDNVKHIFDREVSPLIPGIFHGFNATVLAYGATGSGKTFTMQGIDELPGL 134

Query: 134 MPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLRGLSKV 193
           MPL MS +LS+CE+T  RAE+SYYEVY++RC DLLE K  EI + DDK+GQ+HL+GLS V
Sbjct: 135 MPLTMSTILSMCEKTRSRAEISYYEVYMDRCWDLLEVKDNEIAVWDDKDGQVHLKGLSSV 194

Query: 194 AVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTGKLNLI 253
            V SMSEF+E    GVQRRKVA T LNDVSSRSHGVLVI+V+      S   VTGK+NLI
Sbjct: 195 PVKSMSEFQEAYLCGVQRRKVAHTGLNDVSSRSHGVLVISVT------SQGLVTGKINLI 254

Query: 254 DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRILQDSL 313
           DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNV+YALN NL RVPYRE+KLTRILQDSL
Sbjct: 255 DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVVYALNNNLPRVPYRETKLTRILQDSL 314

Query: 314 GGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQAKVDMESKLRAWL 373
           GGTSRALM+ACLNPG YQES+ TVSLAARSR ISN V    K  TP+ K+DME+KL+AWL
Sbjct: 315 GGTSRALMVACLNPGEYQESLRTVSLAARSRHISNNVSLNPKVETPKVKIDMEAKLQAWL 374

Query: 374 ESKGKTKSAQRIGGIGSPFSSKTPCS-TASKVRKPLFNSSAKVKKTANQSDCKREKERTV 433
           ESKGK KSA R+  I SP       S + S V+K L + SA  +        +R+   T 
Sbjct: 375 ESKGKMKSAHRMMAIRSPLMGTNQTSISQSSVKKLLCHRSAIAESAKLAGTGQRDAFVTA 434

Query: 434 AVAFG--------------NLV--------------ENKDIANSIATKDH---VEK--VK 493
              FG              NL               E   + +  + +D+   VEK   +
Sbjct: 435 RNLFGVETLAASHLWEPIRNLQLASPTKEDERDTSGEENLLVSEASLRDNTLDVEKKYTE 494

Query: 494 FNSPRKALSPIHNTNGIQKAVLEISFTDQLPARTPKTPF-PANEEFKSFGTPLDKFDAQV 553
            +  R+ALSPI +      A     F   L   TPKTPF   N E         KF+A  
Sbjct: 495 LSPLREALSPIDSNAKPNSAHGSSPF---LKPMTPKTPFLSTNPEIMQMEGTCQKFNAWS 554

Query: 554 STLKSCVAQEYVDFLNTASREELVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSK 603
           + LK+ + +EY+ FLNTA+REEL ELKGIG KMAEYI++LRETSPLKSL DL+K+G +S+
Sbjct: 555 NNLKTSLIKEYIHFLNTANREELRELKGIGQKMAEYIIELRETSPLKSLTDLEKIGFTSR 614

BLAST of CmaCh14G001500.1 vs. TAIR 10
Match: AT5G23910.1 (ATP binding microtubule motor family protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.9e-57
Identity = 217/721 (30.10%), Postives = 341/721 (47.30%), Query Frame = 0

Query: 13  TSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNE 72
           TS     VRVVARV+P  S +++   S      ISV        E VT++         +
Sbjct: 2   TSHGRKVVRVVARVKP--STDLASTKS------ISVQKPMGDDSETVTISFGAQFAGSKD 61

Query: 73  CYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQGTEEEPG 132
            Y+LD  + +E+ T   I  KE+ PLI  +F+G +A V A+GA  SGK++ +QG E E G
Sbjct: 62  SYRLDYCY-EENETTGSILTKEIKPLISTVFEGKDANVIAHGARNSGKTHLIQGNERELG 121

Query: 133 LMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLRGLSK 192
           L  L MS +LS+ EE G    +S YEV  E  +DLL+ + + + +++  +G+I L+GLS+
Sbjct: 122 LAVLTMSEMLSMAEERGDAIFVSVYEVSQETVYDLLDQEKRVVSVLEGAQGKIQLKGLSQ 181

Query: 193 VAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTGKLNL 252
           V V S+SEF + L  G ++ +   +DL    +RSH  ++I V++   G++ +   G++N 
Sbjct: 182 VPVKSLSEF-QNLYFGFKKSQKLTSDL---PTRSHKGVMIHVTT---GNANSGSLGRMNF 241

Query: 253 IDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRILQDS 312
           +D+AG ED+R+  N  +   E A++N+S++AL NV+YALN N + VPYRESKLTR+L+D 
Sbjct: 242 LDMAGYEDSRKQ-NSALGPLEIARVNKSIYALQNVMYALNANESHVPYRESKLTRMLKDC 301

Query: 313 LGGTSRALMIACLNPGAYQESVHTVSLAAR-----SRQISNFVVPIHKQ--GTPQAKVDM 372
           L G++  L+I CL     Q+S + ++LA+R     +R I+N   P  K+  G  ++    
Sbjct: 302 LKGSNITLLITCLPREFSQDSFYMLNLASRICLGGNRAITN---PTKKKINGLDRSVSLS 361

Query: 373 ESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTAS---KVRKPLF---NSSAKVKKTA 432
            +  R          S ++    G+    KT  +TA+   K RK LF   N S K K ++
Sbjct: 362 SAAQRRQTPLTMSAASRKQTVLRGNVTERKTKINTATSAIKARK-LFGEANDSVKCKNSS 421

Query: 433 NQSDCKREKERTVAVAFGNLV-------ENKDIANSIATKDH----VEK---VKFNSPRK 492
            + + K +      ++   +V         ++I +SI   D     VE+   + F+S   
Sbjct: 422 KKVEGKAKMVLKKGISTSKVVLSVQASSPKEEICSSITVTDFQSSLVEEEYSLAFSSSTV 481

Query: 493 ALSP------------IHNTNG--------------------IQKAVL------------ 552
           A+ P            I+ T+                     + KA +            
Sbjct: 482 AMEPGYSNGASLSSEAIYTTDKETPRKQEEMFAGATHCDDAFVDKAQIVERDENNSVIEE 541

Query: 553 ----------------------------------EIS--------FTDQLPA----RTPK 593
                                             E+S        F++QL      +TP 
Sbjct: 542 DLTLVIDEGENLDKENNSLLANETASPPLSMRLQELSNNLKSICKFSNQLSVPEKHQTPL 601

BLAST of CmaCh14G001500.1 vs. TAIR 10
Match: AT3G49650.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 190.3 bits (482), Expect = 4.7e-48
Identity = 131/351 (37.32%), Postives = 192/351 (54.70%), Query Frame = 0

Query: 10  APATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETS 69
           APA     + + V  + RP + +E      RV  S   VV    +S + +    +    +
Sbjct: 6   APAAK-KTTTLTVAVKCRPLMEKERGRDIVRVNNSKEVVVLDPDLSKDYLD---RIQNRT 65

Query: 70  RNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQGTEE 129
           + + Y  D  FG E  T + ++ + ++ +I  +  G NATVFAYG+TGSGK+YTM GT  
Sbjct: 66  KEKKYCFDHAFGPES-TNKNVY-RSMSSVISSVVHGLNATVFAYGSTGSGKTYTMVGTRS 125

Query: 130 EPGLMPLAMSRVLSLCEETGCRAEM----SYYEVYLERCHDLLEPKAKEIVIMDDKEGQI 189
           +PGLM L+++ +  + +      E     SY EVY E  +DLLE  +  + + +D E  I
Sbjct: 126 DPGLMVLSLNTIFDMIKSDKSSDEFEVTCSYLEVYNEVIYDLLEKSSGHLELREDPEQGI 185

Query: 190 HLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAAS 249
            + GL  + V+S     E L LG  RRK   T++N  SSRSH VL I V       +   
Sbjct: 186 VVAGLRSIKVHSADRILELLNLGNSRRKTESTEMNGTSSRSHAVLEIAVKRRQKNQNQV- 245

Query: 250 VTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYAL----NKNLARVPYR 309
           + GKL L+DLAG+E    T N G +L++ A IN+SL AL+N I AL     K LA VPYR
Sbjct: 246 MRGKLALVDLAGSERAAETNNGGQKLRDGANINRSLLALANCINALGKQHKKGLAYVPYR 305

Query: 310 ESKLTRILQDSLGGTSRALMIACLNP--GAYQESVHTVSLAARSRQISNFV 351
            SKLTRIL+D L G S+ +M+A ++P    Y  +V+T+  A R+++I   +
Sbjct: 306 NSKLTRILKDGLSGNSQTVMVATISPADSQYHHTVNTLKYADRAKEIKTHI 349

BLAST of CmaCh14G001500.1 vs. TAIR 10
Match: AT2G37420.1 (ATP binding microtubule motor family protein )

HSP 1 Score: 181.8 bits (460), Expect = 1.7e-45
Identity = 124/353 (35.13%), Postives = 188/353 (53.26%), Query Frame = 0

Query: 20  VRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNECYQLDSF 79
           V+V+ R +P   +E      RV +SC  +         EV V         +  +  D  
Sbjct: 49  VQVILRCKPLSEEEQKSSVPRV-ISCNEM-------RREVNVLHTIANKQVDRLFNFDKV 108

Query: 80  FGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQG--------TEEEP 139
           FG +    R I+D+ + P++  + +G + TVFAYG TG+GK+YTM+G           E 
Sbjct: 109 FGPKSQQ-RSIYDQAIAPIVHEVLEGFSCTVFAYGQTGTGKTYTMEGGMRKKGGDLPAEA 168

Query: 140 GLMPLAMSRVLSLCEETGCRAEM--SYYEVYLERCHDLL----------EPKAKEIVIMD 199
           G++P A+  +    E       M  ++ E+Y E   DLL          + + K I +M+
Sbjct: 169 GVIPRAVRHIFDTLEAQNADYSMKVTFLELYNEEVTDLLAQDDSSRSSEDKQRKPISLME 228

Query: 200 DKEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITV--SSP 259
           D +G + LRGL +  V S ++    L  G  +R+ ADT LN  SSRSH V  ITV     
Sbjct: 229 DGKGSVVLRGLEEEVVYSANDIYALLERGSSKRRTADTLLNKRSSRSHSVFTITVHIKEE 288

Query: 260 VCGDSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLAR 319
             GD      GKLNL+DLAG+E+  R+G    R +E+ +IN+SL  L  VI AL ++ + 
Sbjct: 289 SMGDEELIKCGKLNLVDLAGSENILRSGARDGRAREAGEINKSLLTLGRVINALVEHSSH 348

Query: 320 VPYRESKLTRILQDSLGGTSRALMIACLNPGAY--QESVHTVSLAARSRQISN 349
           VPYR+SKLTR+L+DSLGG ++  +IA ++P A+  +E++ T+  A R++ I N
Sbjct: 349 VPYRDSKLTRLLRDSLGGKTKTCIIATISPSAHSLEETLSTLDYAYRAKNIKN 392

BLAST of CmaCh14G001500.1 vs. TAIR 10
Match: AT2G28620.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 181.4 bits (459), Expect = 2.2e-45
Identity = 114/353 (32.29%), Postives = 194/353 (54.96%), Query Frame = 0

Query: 20  VRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNECYQLDSF 79
           ++V+ R RPF S+E  +++  V ++C           +EV V         ++ +  D  
Sbjct: 51  IQVIVRCRPFNSEETRLQTPAV-LTC-------NDRKKEVAVAQNIAGKQIDKTFLFDKV 110

Query: 80  FGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGKSYTMQG--------TEEEP 139
           FG      + ++ + V+P++  +  G N T+FAYG TG+GK+YTM+G           + 
Sbjct: 111 FGPTSQQ-KDLYHQAVSPIVFEVLDGYNCTIFAYGQTGTGKTYTMEGGARKKNGEIPSDA 170

Query: 140 GLMPLAMSRVLSLCEETGC---RAEMSYYEVYLERCHDLLEPK---------AKEIVIMD 199
           G++P A+ ++  + E         ++S+ E+Y E   DLL P+          K + +M+
Sbjct: 171 GVIPRAVKQIFDILEAQSAAEYSLKVSFLELYNEELTDLLAPEETKFADDKSKKPLALME 230

Query: 200 DKEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVC 259
           D +G + +RGL +  V++  E  + L  G  +R+ A+T LN  SSRSH +  +T+    C
Sbjct: 231 DGKGGVFVRGLEEEIVSTADEIYKVLEKGSAKRRTAETLLNKQSSRSHSIFSVTIHIKEC 290

Query: 260 GDSAASV--TGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLAR 319
                 +  +GKLNL+DLAG+E+  R+G    R +E+ +IN+SL  L  VI AL ++   
Sbjct: 291 TPEGEEIVKSGKLNLVDLAGSENISRSGAREGRAREAGEINKSLLTLGRVINALVEHSGH 350

Query: 320 VPYRESKLTRILQDSLGGTSRALMIACLNPGAY--QESVHTVSLAARSRQISN 349
           +PYRESKLTR+L+DSLGG ++  +IA ++P  +  +E++ T+  A R++ I N
Sbjct: 351 IPYRESKLTRLLRDSLGGKTKTCVIATVSPSVHCLEETLSTLDYAHRAKHIKN 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5E9137.5e-16855.93Kinesin-like protein KIN-10B OS=Arabidopsis thaliana OX=3702 GN=KIN10B PE=2 SV=1[more]
Q6L5125.0e-15655.83Kinesin-like protein KIN-10C OS=Oryza sativa subsp. japonica OX=39947 GN=KIN10C ... [more]
Q6P3R12.6e-6733.23Kinesin-like protein KIF22 OS=Xenopus tropicalis OX=8364 GN=kif22 PE=2 SV=1[more]
Q3V3009.7e-6732.28Kinesin-like protein KIF22 OS=Mus musculus OX=10090 GN=Kif22 PE=2 SV=2[more]
Q9I8697.7e-6432.44Kinesin-like protein KIF22-A OS=Xenopus laevis OX=8355 GN=kif22-a PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J7B80.0e+00100.00Kinesin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482032 PE=3 SV=1[more]
A0A6J1J5U20.0e+0099.84Kinesin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482032 PE=3 SV=1[more]
A0A6J1F8V40.0e+0098.85Kinesin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441883 PE=3 SV=1[more]
A0A6J1J2640.0e+0099.51Kinesin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482032 PE=3 SV=1[more]
A0A6J1F2Y60.0e+0098.69Kinesin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441883 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022983424.10.0e+00100.00kinesin-like protein KIN-10B isoform X1 [Cucurbita maxima][more]
XP_022983425.10.0e+0099.84kinesin-like protein KIN-10B isoform X2 [Cucurbita maxima][more]
XP_022934835.10.0e+0098.85kinesin-like protein KIN-10B isoform X1 [Cucurbita moschata][more]
XP_022983426.10.0e+0099.51kinesin-like protein KIN-10B isoform X3 [Cucurbita maxima][more]
XP_022934836.10.0e+0098.69kinesin-like protein KIN-10B isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G02370.15.3e-16955.93ATP binding microtubule motor family protein [more]
AT5G23910.11.9e-5730.10ATP binding microtubule motor family protein [more]
AT3G49650.14.7e-4837.32P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G37420.11.7e-4535.13ATP binding microtubule motor family protein [more]
AT2G28620.12.2e-4532.29P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001752Kinesin motor domainPRINTSPR00380KINESINHEAVYcoord: 249..267
score: 48.86
coord: 298..319
score: 61.86
coord: 105..126
score: 65.95
coord: 217..234
score: 50.0
IPR001752Kinesin motor domainSMARTSM00129kinesin_4coord: 17..355
e-value: 1.6E-98
score: 343.2
IPR001752Kinesin motor domainPFAMPF00225Kinesincoord: 25..346
e-value: 2.7E-87
score: 292.8
IPR001752Kinesin motor domainPROSITEPS50067KINESIN_MOTOR_2coord: 19..346
score: 83.784798
NoneNo IPR availablePFAMPF12836HHH_3coord: 533..579
e-value: 1.2E-6
score: 28.6
NoneNo IPR availableGENE3D1.10.150.280coord: 519..591
e-value: 1.1E-9
score: 39.7
NoneNo IPR availablePANTHERPTHR47969CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATEDcoord: 11..597
NoneNo IPR availablePANTHERPTHR47969:SF9BNACNNG40390D PROTEINcoord: 11..597
NoneNo IPR availableCDDcd01376KISc_KID_likecoord: 20..344
e-value: 1.72818E-146
score: 424.608
IPR036961Kinesin motor domain superfamilyGENE3D3.40.850.10Kinesin motor domaincoord: 9..347
e-value: 7.3E-101
score: 339.4
IPR019821Kinesin motor domain, conserved sitePROSITEPS00411KINESIN_MOTOR_1coord: 248..259
IPR010994RuvA domain 2-likeSUPERFAMILY47781RuvA domain 2-likecoord: 526..590
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 19..374

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh14G001500CmaCh14G001500gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh14G001500.1:exon:433CmaCh14G001500.1:exon:433exon
CmaCh14G001500.1:exon:434CmaCh14G001500.1:exon:434exon
CmaCh14G001500.1:exon:435CmaCh14G001500.1:exon:435exon
CmaCh14G001500.1:exon:436CmaCh14G001500.1:exon:436exon
CmaCh14G001500.1:exon:437CmaCh14G001500.1:exon:437exon
CmaCh14G001500.1:exon:438CmaCh14G001500.1:exon:438exon
CmaCh14G001500.1:exon:439CmaCh14G001500.1:exon:439exon
CmaCh14G001500.1:exon:440CmaCh14G001500.1:exon:440exon
CmaCh14G001500.1:exon:441CmaCh14G001500.1:exon:441exon
CmaCh14G001500.1:exon:442CmaCh14G001500.1:exon:442exon
CmaCh14G001500.1:exon:443CmaCh14G001500.1:exon:443exon
CmaCh14G001500.1:exon:444CmaCh14G001500.1:exon:444exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh14G001500.1:five_prime_utrCmaCh14G001500.1:five_prime_utrfive_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh14G001500.1:cdsCmaCh14G001500.1:cdsCDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_2CDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_3CDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_4CDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_5CDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_6CDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_7CDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_8CDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_9CDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_10CDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_11CDS
CmaCh14G001500.1:cdsCmaCh14G001500.1:cds_12CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh14G001500.1:three_prime_utrCmaCh14G001500.1:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh14G001500.1CmaCh14G001500.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007018 microtubule-based movement
cellular_component GO:0005871 kinesin complex
cellular_component GO:0005874 microtubule
molecular_function GO:0005524 ATP binding
molecular_function GO:0008017 microtubule binding
molecular_function GO:0003777 microtubule motor activity
molecular_function GO:0016887 ATP hydrolysis activity