CmaCh10G000870.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G000870.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionE3 ubiquitin-protein ligase RGLG2-like
LocationCma_Chr10: 356190 .. 359937 (-)
Sequence length1461
RNA-Seq ExpressionCmaCh10G000870.1
SyntenyCmaCh10G000870.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGTTTTTTCAGATTGTTCTCAGCCGAGTGAAATTTTCTACTCTTTTTGTCGATGGGATGGATGATTAAATTCTCTGTTTATCGATATTCTTCTTGCGAAATTTAATCCAAATCTTCGTCAGATCATTGCTCTTGAATATTAGCATTGCTCCTAGTAGATTTTGGGTTTGGATTGCCTCTTGGAGATTTGGGTTTCTTGCAAAAGTTCTCGGAAATCCTTGCATGTACTTGTCTCTGGTATCAAACCACTGAATTTACATGTTCTGTACAAAGATACGAAGCTTTTATCATCATTCAACCTTCTAACAGCAGGGTTGTTTTGTTTGTCTTTCATTTGGACTCTTTCTGATCTTAGAACCCATTTGTAACGGCCCAACCCACTATTAGTAGATATTGTCCTTTTTAGGTTTTCCCTCTCAAGGTTTTAAATCGCGTTTACTAGGGAGAGGTTTCCACAGCTTTATAAATAATGTTTCGTTCTCCTCCACAACTGATGTGCGGTCTCACAATCCATCTCCTTCGGGGCCAGCGTTCTTGCTGGCACTCATTCCTTTCTCCAATTGATGCGGGACCCCTCCAATCCACCCCCTTCGGATCCCAGCGTCCTTGCTAGCACACTGCCTTGTATCCACCCCCTTGGAGGCTTAGTCTTCTCGCTGACACATCGCCTAGACACTCTTATACCATTTGTAACTGTCCAAGTCCACCACTAGCATTTATTGTCCTCTTTGGGCTTTCCCCTTTGAGCTTCCCCTCAAGGCTTTAAAACGCATCTACTAGAGAGAAGTTTCCACACCCTTATAAAGAATACTTTGTTCTCTTCCCCAACCGATGTGGGATCTCATACCATTCATTTCATTTGTATAGTTAAACCTCTTTTGTGTTTTGGGCCTTGTTTTGTGAAACCTAGCTTCCTCTATATATAAAAACACAGAGAATTTCCGGTGAATGATGCTTACAATCAACTTCATGTTGCAGAGTTCTTGTAAAGTGATGGGTGGGAAAAGTTCTAAGCCTGGAAGTTCAAGACGCCATTCGTCTAGTTGGTCTAACTCACAGCAGTGGAGTAATTACAATTATCCGGGGTCGCCATATAATCAACCAAGGCCACAGTATCAGTTTGCACCTCCAACTCCAAGCTATGGTGGAACTCAGGCCCCTGAGACAAGAAAGAGGCTGGAGAGGAAGTATTCAAGGATAGATGATAACTATAATAGTCTTGAGCAGGTCTGCTGCAATTCATATTGCAAAATTTTATTGACACTAAATATTTCAGCATGAAACTCGTTTCTATACTCTGATACTCTTCACAGGTTACTGAGGCATTGGCTCGTGCAGGCCTCGAGTCTTCGAATCTTATCGTTGGTATAGATTTCACAAAGAGCAATGAGTGGACAGGTTCAGCCTTGCTTGGCCATTTAACGTTATAATCTCTTTCTTGCATAGCATGTGCACATGCTAACTATGCCCTTTACATAGGAGCAAGATCATTTAACCGTAGAAGTTTGCATCACATTGGAGATGAGCAAAATCCCTATGAACAAGCAATAGCAATTATTGGGAAAACATTATCTTCCTTTGATGAAGATAATTTGATTCCTTGCTTTGGTTTTGGAGATGGTATTTCTTCTAAAGATTTTTGCAGTTGATCTGTTTTTCGCACATTTTTGTTTGAATAAACAGTAGTGAAGTATGTGACTCTAAATGTTGGCAGCATCGACTCATGACCAAGAAGTTTTCAGCTTTTATCCAGACGAGCGAGTTTGTATTGGATTTGAAGAAGTGTTGGATAGATATAGGGAACTTGTGCCTCATCTTCGACTTGCAGGTTGCAGTCTGACCATTTGCTATTATACCTTTCTGTTGTTGGCTTTTACTTACTGTACTTTCATTATTTCATTCCTGGGACAGGACCAACATCATTTGCTCCTATTATCGAAATGGGTATCACAATTGTAGAGCAAAGTGGAGGACAATATCATGTATTGGTAATAATAGCTGATGGTCAGGTACTTCCATCTCCATTCCAACTTGACTTCAATTTTGATTGTTTTAGAGCTCATGTTTATAGCATAACTTCCTGTTTGCTCCCAGATGCATAAGAAATTAGTAGTTATGTAAATGATAATAGTTTCACAATGAGCAGTGAAAATATGGAACCATTTAATTTCAGCCTAAAGAACAGAAACAAAAGTTGCATCTGCATCGATTATGTAACAACTCAAGCCCACCGCTAGCAAATATTGTCCTCTTTAGACTTTTCCTTTCGGACTTCTCCTCAAGGTTTTTAGAACTCGTCTGCTAGGGAGATGTTTCCACACTCTTATAAAGAATGTTTCATTCCCCTTTTCAACCGATGTGAGATCTCAAGGTTGTGGGCAGGAACAATGTGTCAAAAATTCTAATTTTTGAAGGATGTAGAAATCTTCTCATTCATAAATCATGGCTGATTAAACTGATATGAGTCAGTTTTATTTATCAAGCAATACACTGCTTGTTCAGTAAGCTATTCATAATGTATGTAGTCTTTAAATAGAGTTTCCAGCCTGACTGATGTCTGTGGCGAGTACTAACTAAAGCCAAGTTTTCTCATGGTGGTTGAAAAATAGTCTTTATTCTCTTTGACAGGTTACAAGAAGTGTTGATACAGAGGGTGGACAGTTTAGCCCACAGGAAAAGAAAACCATTGAAGCAATTGTGAAAGCTAGGTACGTTAATTTATCAATAATGGAACAGAAATGGCATTATTCGTGCTCTGTAACTTCATGTTGAATTGCAGTGCATATCCCCTGTCGATTATACTGGTCGGAGTAGGGGATGGACCTTGGGACATGATGAGGGAATTTGACGACAATATCCCGGCTCGAGCCTTTGATAATTTCCAAGTAACTACTCTTCTTAGGAGGGTTTTAGTGTTTTGTTTCTCAAACTGGACTTGCTTCTGTCATAGCTTGAAATCTGAGAAATTCCATTTTGCACTGCAGTTTGTGAACTTCACAAATATAATGTTGAAAAATATGGATCGAGCTAGAAAGGAAACGGAATTTGCTCTAGCAGCACTCATGGAAATACCCTCCCAGTATAAAGCAACACTGGAGCTTGATATTTTGGGGTAATAACAGGATTCAAAAGTAGTATATTGTAAGATTCTTGCAGTTAAATGGTAGTGTATTTCCTTCCATGATTTTTTTCTTAAATAGTATTCTCTTTCACTACTCCACCAGTGCCAGTAGAGGGAAGGAAATCGACCGATTTCCTCTTCCCCCTCCTCGTTATGGTCCTAGTTTCCAGAAGACCTCAAACTCGAGCAGCTCTCGCCCAATGCCACCCTCATTTGCTGGAGTTGCTCCTGTCCGATCTGCACCTCCTCTAAGTTCGGTTCCTGATGCCCATGTAAATGCTTCTTTTCCCTTATTAGATCCATATTATCTCAGCAATGAAGACTGAGTAGAGCTTCTATTCTACTGGGTATGAAAATTGAAAAATGGAAATGAAGATTTTTACACCATCTGTTGCAGGCTTGTCCCATTTGCATCTCCAATGCAAAAGATATGGCTTTTGGTTGTGGACATCAGGTAATCGCATTGGTAACTTTCATATTGAAGCTTTTGATGCCTGACTCAATCATTACCTGGAAGAAACACTAAACTTTTACTATCTGCAGACATGCTGTGAATGTGGACAGGATCTTCAGTTGTGCCCCATATGCCGCAGCTTTATTGAAACTAAGATAAAGCTTTATTAG

mRNA sequence

ATGCCGTTTTTTCAGATTGTTCTCAGCCGAAGTTCTTGTAAAGTGATGGGTGGGAAAAGTTCTAAGCCTGGAAGTTCAAGACGCCATTCGTCTAGTTGGTCTAACTCACAGCAGTGGAGTAATTACAATTATCCGGGGTCGCCATATAATCAACCAAGGCCACAGTATCAGTTTGCACCTCCAACTCCAAGCTATGGTGGAACTCAGGCCCCTGAGACAAGAAAGAGGCTGGAGAGGAAGTATTCAAGGATAGATGATAACTATAATAGTCTTGAGCAGGTTACTGAGGCATTGGCTCGTGCAGGCCTCGAGTCTTCGAATCTTATCGTTGGTATAGATTTCACAAAGAGCAATGAGTGGACAGGAGCAAGATCATTTAACCGTAGAAGTTTGCATCACATTGGAGATGAGCAAAATCCCTATGAACAAGCAATAGCAATTATTGGGAAAACATTATCTTCCTTTGATGAAGATAATTTGATTCCTTGCTTTGGTTTTGGAGATGCATCGACTCATGACCAAGAAGTTTTCAGCTTTTATCCAGACGAGCGAGTTTGTATTGGATTTGAAGAAGTGTTGGATAGATATAGGGAACTTGTGCCTCATCTTCGACTTGCAGGACCAACATCATTTGCTCCTATTATCGAAATGGGTATCACAATTGTAGAGCAAAGTGGAGGACAATATCATGTATTGGTAATAATAGCTGATGGTCAGGTTACAAGAAGTGTTGATACAGAGGGTGGACAGTTTAGCCCACAGGAAAAGAAAACCATTGAAGCAATTGTGAAAGCTAGTGCATATCCCCTGTCGATTATACTGGTCGGAGTAGGGGATGGACCTTGGGACATGATGAGGGAATTTGACGACAATATCCCGGCTCGAGCCTTTGATAATTTCCAAGTAACTACTCTTCTTAGGAGGGTTTTAGTGTTTTGTTTCTCAAACTGGACTTGCTTCTGTCATAGCTTGAAATCTGAGAAATTCCATTTTGCACTGCAGTTTGTGAACTTCACAAATATAATGTTGAAAAATATGGATCGAGCTAGAAAGGAAACGGAATTTGCTCTAGCAGCACTCATGGAAATACCCTCCCAGTATAAAGCAACACTGGAGCTTGATATTTTGGGTATTCTCTTTCACTACTCCACCAGTGCCAGTAGAGGGAAGGAAATCGACCGATTTCCTCTTCCCCCTCCTCGTTATGGTCCTAGTTTCCAGAAGACCTCAAACTCGAGCAGCTCTCGCCCAATGCCACCCTCATTTGCTGGAGTTGCTCCTGTCCGATCTGCACCTCCTCTAAGTTCGGTTCCTGATGCCCATGCTTGTCCCATTTGCATCTCCAATGCAAAAGATATGGCTTTTGGTTGTGGACATCAGACATGCTGTGAATGTGGACAGGATCTTCAGTTGTGCCCCATATGCCGCAGCTTTATTGAAACTAAGATAAAGCTTTATTAG

Coding sequence (CDS)

ATGCCGTTTTTTCAGATTGTTCTCAGCCGAAGTTCTTGTAAAGTGATGGGTGGGAAAAGTTCTAAGCCTGGAAGTTCAAGACGCCATTCGTCTAGTTGGTCTAACTCACAGCAGTGGAGTAATTACAATTATCCGGGGTCGCCATATAATCAACCAAGGCCACAGTATCAGTTTGCACCTCCAACTCCAAGCTATGGTGGAACTCAGGCCCCTGAGACAAGAAAGAGGCTGGAGAGGAAGTATTCAAGGATAGATGATAACTATAATAGTCTTGAGCAGGTTACTGAGGCATTGGCTCGTGCAGGCCTCGAGTCTTCGAATCTTATCGTTGGTATAGATTTCACAAAGAGCAATGAGTGGACAGGAGCAAGATCATTTAACCGTAGAAGTTTGCATCACATTGGAGATGAGCAAAATCCCTATGAACAAGCAATAGCAATTATTGGGAAAACATTATCTTCCTTTGATGAAGATAATTTGATTCCTTGCTTTGGTTTTGGAGATGCATCGACTCATGACCAAGAAGTTTTCAGCTTTTATCCAGACGAGCGAGTTTGTATTGGATTTGAAGAAGTGTTGGATAGATATAGGGAACTTGTGCCTCATCTTCGACTTGCAGGACCAACATCATTTGCTCCTATTATCGAAATGGGTATCACAATTGTAGAGCAAAGTGGAGGACAATATCATGTATTGGTAATAATAGCTGATGGTCAGGTTACAAGAAGTGTTGATACAGAGGGTGGACAGTTTAGCCCACAGGAAAAGAAAACCATTGAAGCAATTGTGAAAGCTAGTGCATATCCCCTGTCGATTATACTGGTCGGAGTAGGGGATGGACCTTGGGACATGATGAGGGAATTTGACGACAATATCCCGGCTCGAGCCTTTGATAATTTCCAAGTAACTACTCTTCTTAGGAGGGTTTTAGTGTTTTGTTTCTCAAACTGGACTTGCTTCTGTCATAGCTTGAAATCTGAGAAATTCCATTTTGCACTGCAGTTTGTGAACTTCACAAATATAATGTTGAAAAATATGGATCGAGCTAGAAAGGAAACGGAATTTGCTCTAGCAGCACTCATGGAAATACCCTCCCAGTATAAAGCAACACTGGAGCTTGATATTTTGGGTATTCTCTTTCACTACTCCACCAGTGCCAGTAGAGGGAAGGAAATCGACCGATTTCCTCTTCCCCCTCCTCGTTATGGTCCTAGTTTCCAGAAGACCTCAAACTCGAGCAGCTCTCGCCCAATGCCACCCTCATTTGCTGGAGTTGCTCCTGTCCGATCTGCACCTCCTCTAAGTTCGGTTCCTGATGCCCATGCTTGTCCCATTTGCATCTCCAATGCAAAAGATATGGCTTTTGGTTGTGGACATCAGACATGCTGTGAATGTGGACAGGATCTTCAGTTGTGCCCCATATGCCGCAGCTTTATTGAAACTAAGATAAAGCTTTATTAG

Protein sequence

MPFFQIVLSRSSCKVMGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVLVFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLSSVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY
Homology
BLAST of CmaCh10G000870.1 vs. ExPASy Swiss-Prot
Match: Q9LY87 (E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.7e-154
Identity = 302/505 (59.80%), Postives = 351/505 (69.50%), Query Frame = 0

Query: 9   SRSSCKVMGGKSSKPGSSRRHSSSWSNSQQW-----SNYNYPGSP-YNQPRPQYQFAPP- 68
           S+ + +    +S+   S+   SSSW++ Q +      +YNYP  P Y QP P+Y   PP 
Sbjct: 6   SKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQP-PEYTQPPPP 65

Query: 69  --------TPSYGG----TQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLI 128
                    PSY      +   + +KRLERKYS+I D+Y+SLEQVTEALARAGLESSNLI
Sbjct: 66  LYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLI 125

Query: 129 VGIDFTKSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDA 188
           VGIDFTKSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDNLIPC+GFGDA
Sbjct: 126 VGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDA 185

Query: 189 STHDQEVFSFYPDERVCIGFEEVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQY 248
           STHDQ+VFSF  ++R C GFEEVL RY+E+VP L+LAGPTSFAPII+M +TIVEQSGGQY
Sbjct: 186 STHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQY 245

Query: 249 HVLVIIADGQVTRSVDTEGGQFSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFD 308
           HVLVIIADGQVTRSVDTE GQ SPQE+KT++AIV+AS  PLSI+LVGVGDGPWDMMREFD
Sbjct: 246 HVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFD 305

Query: 309 DNIPARAFDNFQVTTLLRRVLVFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRA 368
           DNIPARAFDNF                                 QFVNFT IM KN  ++
Sbjct: 306 DNIPARAFDNF---------------------------------QFVNFTEIMAKNKAQS 365

Query: 369 RKETEFALAALMEIPSQYKATLELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKT 428
            KETEFAL+ALMEIP QYKAT+EL++LG           G   +RFPLPPP  G S    
Sbjct: 366 LKETEFALSALMEIPQQYKATIELNLLG--------RRNGYIPERFPLPPPMRGGSSSYN 425

Query: 429 SNSSSSRP-----MPPSFAGVAPVRSA---PPLSSVPDAHACPICISNAKDMAFGCGHQT 487
           S   S  P     +PP       VRS+   PP SS  D   CPIC+SN KDMAFGCGHQT
Sbjct: 426 SPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLSNPKDMAFGCGHQT 468

BLAST of CmaCh10G000870.1 vs. ExPASy Swiss-Prot
Match: Q9SS90 (E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.4e-148
Identity = 297/536 (55.41%), Postives = 347/536 (64.74%), Query Frame = 0

Query: 16  MGGKSSKPGSSRRHSSSWSNSQQW-----SNYNYP------------------------- 75
           MGG +SK  SS   SSSW++ Q +      +YNYP                         
Sbjct: 1   MGGGNSKEESSSPSSSSWASHQSYPQYGPDSYNYPPPPTYAPAPSPAPAPAPVPAPSPAS 60

Query: 76  ------------GSPYNQPRPQYQFAP-PTPSYGGTQAPE-------------------- 135
                         P N P P Y  AP P  SYG   + E                    
Sbjct: 61  SYGPQYSQEGYASQPNNPPPPTYAPAPSPASSYGHQYSQEGYASAASQPNYPPPPSQSQV 120

Query: 136 -TRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSL 195
             RK+ +R+YS+I DNY+SL QV+EAL RAGLESSNLIVGIDFTKSNEWTGA+SFNR+SL
Sbjct: 121 ADRKKFDRRYSKISDNYSSLLQVSEALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSL 180

Query: 196 HHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEE 255
           HH+ +  NPYEQAI IIG+TL++FDEDNLIPC+GFGDASTHDQ+VFSFYP+ R C GFEE
Sbjct: 181 HHLSNTPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEE 240

Query: 256 VLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQF 315
           VL RYRE+VP L+LAGPTSFAPIIEM +T+VEQS GQYHVLVIIADGQVTRSVDTE G+ 
Sbjct: 241 VLARYREIVPQLKLAGPTSFAPIIEMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRL 300

Query: 316 SPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVLV 375
           SPQE+KT++AIVKAS  PLSI+LVGVGDGPWDMM+EFDDNIPARAFDNF           
Sbjct: 301 SPQEQKTVDAIVKASTLPLSIVLVGVGDGPWDMMQEFDDNIPARAFDNF----------- 360

Query: 376 FCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATL 435
                                 QFVNFT IM KN D++RKETEFAL+ALMEIP QYKAT+
Sbjct: 361 ----------------------QFVNFTEIMSKNKDQSRKETEFALSALMEIPPQYKATI 420

Query: 436 ELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVR-S 487
           EL++LG+          G    R PLPPP      Q  S+ SSSR +P     V P    
Sbjct: 421 ELNLLGV--------RNGNIPQRIPLPPP-----VQSGSSFSSSR-IPNFEPSVPPYPFE 480

BLAST of CmaCh10G000870.1 vs. ExPASy Swiss-Prot
Match: Q8LB88 (E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 2.4e-140
Identity = 282/481 (58.63%), Postives = 332/481 (69.02%), Query Frame = 0

Query: 16  MGGKSSKP-----GSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQA 75
           MGG SSK      GS RR+  S S S  +S+  +  S Y Q  P +  A P  SY   + 
Sbjct: 1   MGGSSSKESPRGGGSGRRYERSVSGSSSYSS-AWDQSSYYQ-TPNHPSASPVSSYNSGR- 60

Query: 76  PETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRS 135
            +T K LERKYSRI DNY S+++VT AL+ AGLESSNLIVGID TKSNEWTGARSF R+S
Sbjct: 61  -QTPKNLERKYSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARSFGRKS 120

Query: 136 LHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFE 195
           LH IG   NPY+QAI+IIGKTLS FDEDNLIPC+GFGDA+THDQ+VFSF P++  C GFE
Sbjct: 121 LHFIGTTPNPYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTYCNGFE 180

Query: 196 EVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQ 255
           EVL  YRE+VP LRL+GPTSFAPIIE  +TIVE+SGGQYHVL+IIADGQVTRSVDT+ G 
Sbjct: 181 EVLMCYREIVPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVDTDNGG 240

Query: 256 FSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVL 315
           FSPQE++TI+AIV+AS YPLSI+LVGVGDGPWD MR+FDDNIPARAFDNF          
Sbjct: 241 FSPQEQQTIDAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNF---------- 300

Query: 316 VFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKAT 375
                                  QFVNFT+IM KN+D ARKE EFAL+ALMEIPSQYKAT
Sbjct: 301 -----------------------QFVNFTDIMSKNIDPARKEAEFALSALMEIPSQYKAT 360

Query: 376 LELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTS---NSSSSRPMPPSFAGVAP 435
           LEL +LG           G   DR  LPPP Y     + S   + S+S    P   GV+ 
Sbjct: 361 LELGLLG--------QRTGHCPDRIALPPPTYATQSMRNSPRTSRSTSFQNKPYDNGVS- 420

Query: 436 VRSAPPLSSVPDAHA--CPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKL 487
             S PP ++  ++    CP+C+ +AK+MAF CGHQTC  CG+DL +CPICRS I  +IKL
Sbjct: 421 --STPPSTTHNESQQQFCPVCLVSAKNMAFNCGHQTCAGCGEDLHVCPICRSSISVRIKL 433

BLAST of CmaCh10G000870.1 vs. ExPASy Swiss-Prot
Match: Q8RX26 (E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 8.4e-114
Identity = 222/405 (54.81%), Postives = 266/405 (65.68%), Query Frame = 0

Query: 82  SRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGDEQNPY 141
           S I D+++SL+QV  +L  AGLESSNLI+GIDFTKSNEWTG  SFNR+SLH IG  QNPY
Sbjct: 12  SYIADHFSSLDQVITSLREAGLESSNLILGIDFTKSNEWTGRYSFNRKSLHAIGKRQNPY 71

Query: 142 EQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDRYRELVP 201
           E+AI+IIG+TLS FDED+LIPCFGFGD +T DQ VFSFYP+ + C G E  + RYRE+VP
Sbjct: 72  EKAISIIGRTLSPFDEDDLIPCFGFGDVTTRDQYVFSFYPENKSCDGLENAVKRYREIVP 131

Query: 202 HLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQEKKTIEA 261
           HL+L+GPTSFAP+I+  I IVEQ+  QYHVLVIIADGQVTR+ D   G+ SPQE+ T+ +
Sbjct: 132 HLKLSGPTSFAPVIDAAINIVEQNNMQYHVLVIIADGQVTRNPDVPLGRLSPQEEATMNS 191

Query: 262 IVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVLVFCFSNWTCFC 321
           I+ AS YPLSI+LVGVGDGPWD M++FDDNIP R FDNF                     
Sbjct: 192 IMAASHYPLSIVLVGVGDGPWDTMKQFDDNIPHREFDNF--------------------- 251

Query: 322 HSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDILGILFH 381
                       QFVNFT IM ++ D A+KE  FALAALMEIP QYKATL L        
Sbjct: 252 ------------QFVNFTKIMSEHKDAAKKEAAFALAALMEIPFQYKATLSL-------- 311

Query: 382 YSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLSSVPDAH 441
            +    R       PLPPP   P   +  N+  S P   +       R AP  S+VP   
Sbjct: 312 -NRKPVRSSHQHHKPLPPP---PEVIERDNAVRSVPNQMTETAEKSDRLAP--STVP--- 366

Query: 442 ACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 487
            CPIC++N KDMAF CGH TC ECG  +  CP+CR  I T+I+LY
Sbjct: 372 VCPICLTNPKDMAFSCGHTTCKECGVVITTCPLCRQPITTRIRLY 366

BLAST of CmaCh10G000870.1 vs. ExPASy Swiss-Prot
Match: Q9SAL0 (E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.8e-109
Identity = 207/426 (48.59%), Postives = 265/426 (62.21%), Query Frame = 0

Query: 61  PTPSYGGTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEW 120
           P+PS       +     ++KY+ I D ++SL+QV++AL  AGLESSNLI+G+DFTKSNEW
Sbjct: 33  PSPSDPSLSLADNTNATKKKYALIPDRFSSLDQVSKALREAGLESSNLILGVDFTKSNEW 92

Query: 121 TGARSFNRRSLHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFY 180
           TG  SF+ + LH +G+  NPYE+AI +IG+TL+ FDEDNLIPCFGFGD++THD+EVF F+
Sbjct: 93  TGKTSFDGKCLHALGETSNPYEKAIFVIGQTLAPFDEDNLIPCFGFGDSTTHDEEVFGFH 152

Query: 181 PDERVCIGFEEVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQV 240
            D   C GFEEVL  Y+ + P+LRL+GPTS+ P+I+  + IVE++ GQ+HVLVI+ADGQV
Sbjct: 153 SDNSPCHGFEEVLACYKRIAPNLRLSGPTSYGPLIDAAVDIVEKNNGQFHVLVIVADGQV 212

Query: 241 TRSVDTEGGQFSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF 300
           TR  D   G+ S QEK TI+AIV AS+Y LSI+LVGVGDGPW+ MR+FDD IP R FDNF
Sbjct: 213 TRGTDMAEGELSQQEKTTIDAIVNASSYALSIVLVGVGDGPWEDMRKFDDKIPKREFDNF 272

Query: 301 QVTTLLRRVLVFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAAL 360
                                            QFVNFT IM +N   + KET FALAAL
Sbjct: 273 ---------------------------------QFVNFTEIMTRNSPESAKETAFALAAL 332

Query: 361 MEIPSQYKATLELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPP 420
           MEIP QY+A +EL +LG           G      P PPP                P  P
Sbjct: 333 MEIPFQYQAAIELRLLG--------KQTGLAKTIVPRPPP---------------IPYTP 392

Query: 421 SFAGVAPVRSAPPLSSVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIE 480
                 P  ++P  +S     +CPIC++N KD+AF CGH TC +CG  +  CPICR  I 
Sbjct: 393 PTNAELPSTASP--ASPEQTQSCPICLTNRKDVAFSCGHMTCGDCGSKISNCPICRVRIT 400

Query: 481 TKIKLY 487
            ++KLY
Sbjct: 453 NRLKLY 400

BLAST of CmaCh10G000870.1 vs. ExPASy TrEMBL
Match: A0A6J1J9U1 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucurbita maxima OX=3661 GN=LOC111484852 PE=4 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.3e-242
Identity = 430/471 (91.30%), Postives = 430/471 (91.30%), Query Frame = 0

Query: 16  MGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK 75
           MGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK
Sbjct: 1   MGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK 60

Query: 76  RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG 135
           RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG
Sbjct: 61  RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG 120

Query: 136 DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDR 195
           DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDR
Sbjct: 121 DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDR 180

Query: 196 YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE 255
           YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE
Sbjct: 181 YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE 240

Query: 256 KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVLVFCFS 315
           KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF               
Sbjct: 241 KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF--------------- 300

Query: 316 NWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI 375
                             QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI
Sbjct: 301 ------------------QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI 360

Query: 376 LGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLS 435
           LG        ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLS
Sbjct: 361 LG--------ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLS 420

Query: 436 SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 487
           SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY
Sbjct: 421 SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 430

BLAST of CmaCh10G000870.1 vs. ExPASy TrEMBL
Match: A0A6J1E1J1 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucurbita moschata OX=3662 GN=LOC111429937 PE=4 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 1.1e-238
Identity = 425/471 (90.23%), Postives = 428/471 (90.87%), Query Frame = 0

Query: 16  MGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK 75
           MGGKSSKPGSSRRHSSS SNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK
Sbjct: 1   MGGKSSKPGSSRRHSSSRSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK 60

Query: 76  RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG 135
           RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG
Sbjct: 61  RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG 120

Query: 136 DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDR 195
           DEQNPYEQAI+IIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPD+RVCIGFEEVLDR
Sbjct: 121 DEQNPYEQAISIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDDRVCIGFEEVLDR 180

Query: 196 YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE 255
           YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE
Sbjct: 181 YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE 240

Query: 256 KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVLVFCFS 315
           KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF               
Sbjct: 241 KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF--------------- 300

Query: 316 NWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI 375
                             QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI
Sbjct: 301 ------------------QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI 360

Query: 376 LGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLS 435
           LG        ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMP SFAGVAPVRSAPPLS
Sbjct: 361 LG--------ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPHSFAGVAPVRSAPPLS 420

Query: 436 SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 487
           SVPDAHACPICISNAK+MAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY
Sbjct: 421 SVPDAHACPICISNAKNMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 430

BLAST of CmaCh10G000870.1 vs. ExPASy TrEMBL
Match: A0A1S4E4Q6 (E3 ubiquitin-protein ligase RGLG2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502363 PE=4 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 4.5e-219
Identity = 395/480 (82.29%), Postives = 413/480 (86.04%), Query Frame = 0

Query: 7   VLSRSSCKVMGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYG 66
           V+S SSC+VMGGKSSK GSSRR+SSS SNSQQW +Y YP SPY Q R   ++APPTPSYG
Sbjct: 28  VVSFSSCEVMGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRYAPPTPSYG 87

Query: 67  GTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSF 126
           GTQAPETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSF
Sbjct: 88  GTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSF 147

Query: 127 NRRSLHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVC 186
           NRRSLHHIGDEQNPYEQAIAIIG+TLSSFDEDN+IPCFGFGDASTHDQEVFSFY DER C
Sbjct: 148 NRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFC 207

Query: 187 IGFEEVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDT 246
            GFEEVL RY+ELVPHLRLAGPTSFAPIIEM ITIVEQSGGQYHVLVIIADGQVTRSVDT
Sbjct: 208 NGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT 267

Query: 247 EGGQFSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLL 306
           E GQFSPQEKKTIEAIVKAS YPLSIILVGVGDGPWDMMREFDDNIPARAFDNF      
Sbjct: 268 ERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF------ 327

Query: 307 RRVLVFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQ 366
                                      QFVNFTNIMLKN+DR+RKE EFALAALMEIPSQ
Sbjct: 328 ---------------------------QFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQ 387

Query: 367 YKATLELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVA 426
           YKATLEL +LG+        SRGK IDR PLPPP YGPS QKTSNSSSSRP PPSF+GVA
Sbjct: 388 YKATLELGLLGV--------SRGKAIDRIPLPPPFYGPSIQKTSNSSSSRPTPPSFSGVA 447

Query: 427 PVRSAPPLSSVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 486
           PVR+APPLSSV DAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFI+T+IKLY
Sbjct: 448 PVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 466

BLAST of CmaCh10G000870.1 vs. ExPASy TrEMBL
Match: A0A1S4E4Q2 (E3 ubiquitin-protein ligase RGLG2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103502363 PE=4 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 2.9e-218
Identity = 393/476 (82.56%), Postives = 410/476 (86.13%), Query Frame = 0

Query: 11  SSCKVMGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQA 70
           SSC+VMGGKSSK GSSRR+SSS SNSQQW +Y YP SPY Q R   ++APPTPSYGGTQA
Sbjct: 18  SSCEVMGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRYAPPTPSYGGTQA 77

Query: 71  PETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRS 130
           PETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRS
Sbjct: 78  PETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRS 137

Query: 131 LHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFE 190
           LHHIGDEQNPYEQAIAIIG+TLSSFDEDN+IPCFGFGDASTHDQEVFSFY DER C GFE
Sbjct: 138 LHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFE 197

Query: 191 EVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQ 250
           EVL RY+ELVPHLRLAGPTSFAPIIEM ITIVEQSGGQYHVLVIIADGQVTRSVDTE GQ
Sbjct: 198 EVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQ 257

Query: 251 FSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVL 310
           FSPQEKKTIEAIVKAS YPLSIILVGVGDGPWDMMREFDDNIPARAFDNF          
Sbjct: 258 FSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF---------- 317

Query: 311 VFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKAT 370
                                  QFVNFTNIMLKN+DR+RKE EFALAALMEIPSQYKAT
Sbjct: 318 -----------------------QFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYKAT 377

Query: 371 LELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRS 430
           LEL +LG+        SRGK IDR PLPPP YGPS QKTSNSSSSRP PPSF+GVAPVR+
Sbjct: 378 LELGLLGV--------SRGKAIDRIPLPPPFYGPSIQKTSNSSSSRPTPPSFSGVAPVRT 437

Query: 431 APPLSSVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 487
           APPLSSV DAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFI+T+IKLY
Sbjct: 438 APPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 452

BLAST of CmaCh10G000870.1 vs. ExPASy TrEMBL
Match: A0A1S4E5G0 (E3 ubiquitin-protein ligase RGLG2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502363 PE=4 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 2.9e-218
Identity = 393/476 (82.56%), Postives = 410/476 (86.13%), Query Frame = 0

Query: 11  SSCKVMGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQA 70
           SSC+VMGGKSSK GSSRR+SSS SNSQQW +Y YP SPY Q R   ++APPTPSYGGTQA
Sbjct: 28  SSCEVMGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRYAPPTPSYGGTQA 87

Query: 71  PETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRS 130
           PETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRS
Sbjct: 88  PETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRS 147

Query: 131 LHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFE 190
           LHHIGDEQNPYEQAIAIIG+TLSSFDEDN+IPCFGFGDASTHDQEVFSFY DER C GFE
Sbjct: 148 LHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFE 207

Query: 191 EVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQ 250
           EVL RY+ELVPHLRLAGPTSFAPIIEM ITIVEQSGGQYHVLVIIADGQVTRSVDTE GQ
Sbjct: 208 EVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQ 267

Query: 251 FSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVL 310
           FSPQEKKTIEAIVKAS YPLSIILVGVGDGPWDMMREFDDNIPARAFDNF          
Sbjct: 268 FSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF---------- 327

Query: 311 VFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKAT 370
                                  QFVNFTNIMLKN+DR+RKE EFALAALMEIPSQYKAT
Sbjct: 328 -----------------------QFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYKAT 387

Query: 371 LELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRS 430
           LEL +LG+        SRGK IDR PLPPP YGPS QKTSNSSSSRP PPSF+GVAPVR+
Sbjct: 388 LELGLLGV--------SRGKAIDRIPLPPPFYGPSIQKTSNSSSSRPTPPSFSGVAPVRT 447

Query: 431 APPLSSVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 487
           APPLSSV DAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFI+T+IKLY
Sbjct: 448 APPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 462

BLAST of CmaCh10G000870.1 vs. NCBI nr
Match: XP_022987237.1 (E3 ubiquitin-protein ligase RGLG2-like [Cucurbita maxima])

HSP 1 Score: 848.6 bits (2191), Expect = 2.7e-242
Identity = 430/471 (91.30%), Postives = 430/471 (91.30%), Query Frame = 0

Query: 16  MGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK 75
           MGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK
Sbjct: 1   MGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK 60

Query: 76  RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG 135
           RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG
Sbjct: 61  RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG 120

Query: 136 DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDR 195
           DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDR
Sbjct: 121 DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDR 180

Query: 196 YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE 255
           YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE
Sbjct: 181 YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE 240

Query: 256 KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVLVFCFS 315
           KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF               
Sbjct: 241 KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF--------------- 300

Query: 316 NWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI 375
                             QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI
Sbjct: 301 ------------------QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI 360

Query: 376 LGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLS 435
           LG        ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLS
Sbjct: 361 LG--------ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLS 420

Query: 436 SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 487
           SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY
Sbjct: 421 SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 430

BLAST of CmaCh10G000870.1 vs. NCBI nr
Match: KAG6589412.1 (E3 ubiquitin-protein ligase RGLG2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 839.0 bits (2166), Expect = 2.1e-239
Identity = 428/476 (89.92%), Postives = 432/476 (90.76%), Query Frame = 0

Query: 11  SSCKVMGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQA 70
           SSCKVMGGKSSKPGSSRR+SSS SNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQA
Sbjct: 19  SSCKVMGGKSSKPGSSRRYSSSRSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQA 78

Query: 71  PETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRS 130
           PETRKRLERKYSRIDDNY SLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRS
Sbjct: 79  PETRKRLERKYSRIDDNYISLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRS 138

Query: 131 LHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFE 190
           LHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPD+RVCIGFE
Sbjct: 139 LHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDDRVCIGFE 198

Query: 191 EVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQ 250
           EVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQ
Sbjct: 199 EVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQ 258

Query: 251 FSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVL 310
           FSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF          
Sbjct: 259 FSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF---------- 318

Query: 311 VFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKAT 370
                                  QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKAT
Sbjct: 319 -----------------------QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKAT 378

Query: 371 LELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRS 430
           LELDILG        ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRP+P SFAGVAPVRS
Sbjct: 379 LELDILG--------ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPIPHSFAGVAPVRS 438

Query: 431 APPLSSVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 487
           APPLSSVPDAHACPICISNAK+MAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY
Sbjct: 439 APPLSSVPDAHACPICISNAKNMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 453

BLAST of CmaCh10G000870.1 vs. NCBI nr
Match: XP_023515574.1 (E3 ubiquitin-protein ligase RGLG2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 838.6 bits (2165), Expect = 2.8e-239
Identity = 427/471 (90.66%), Postives = 428/471 (90.87%), Query Frame = 0

Query: 16  MGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK 75
           MGGKSSKPGSSRRHSSS SNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK
Sbjct: 1   MGGKSSKPGSSRRHSSSRSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK 60

Query: 76  RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG 135
           RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG
Sbjct: 61  RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG 120

Query: 136 DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDR 195
           DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPD+RVCIGFEEVLDR
Sbjct: 121 DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDDRVCIGFEEVLDR 180

Query: 196 YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE 255
           YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE
Sbjct: 181 YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE 240

Query: 256 KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVLVFCFS 315
           KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF               
Sbjct: 241 KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF--------------- 300

Query: 316 NWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI 375
                             QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI
Sbjct: 301 ------------------QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI 360

Query: 376 LGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLS 435
           LG        ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMP SFAGVAPVRSAPPLS
Sbjct: 361 LG--------ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPRSFAGVAPVRSAPPLS 420

Query: 436 SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 487
           SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY
Sbjct: 421 SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 430

BLAST of CmaCh10G000870.1 vs. NCBI nr
Match: XP_022921789.1 (E3 ubiquitin-protein ligase RGLG2-like [Cucurbita moschata])

HSP 1 Score: 835.5 bits (2157), Expect = 2.4e-238
Identity = 425/471 (90.23%), Postives = 428/471 (90.87%), Query Frame = 0

Query: 16  MGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK 75
           MGGKSSKPGSSRRHSSS SNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK
Sbjct: 1   MGGKSSKPGSSRRHSSSRSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYGGTQAPETRK 60

Query: 76  RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG 135
           RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG
Sbjct: 61  RLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIG 120

Query: 136 DEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEEVLDR 195
           DEQNPYEQAI+IIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPD+RVCIGFEEVLDR
Sbjct: 121 DEQNPYEQAISIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDDRVCIGFEEVLDR 180

Query: 196 YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE 255
           YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE
Sbjct: 181 YRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQFSPQE 240

Query: 256 KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVLVFCFS 315
           KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF               
Sbjct: 241 KKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF--------------- 300

Query: 316 NWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI 375
                             QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI
Sbjct: 301 ------------------QFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATLELDI 360

Query: 376 LGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVRSAPPLS 435
           LG        ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMP SFAGVAPVRSAPPLS
Sbjct: 361 LG--------ASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPHSFAGVAPVRSAPPLS 420

Query: 436 SVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 487
           SVPDAHACPICISNAK+MAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY
Sbjct: 421 SVPDAHACPICISNAKNMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 430

BLAST of CmaCh10G000870.1 vs. NCBI nr
Match: XP_016903209.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X1 [Cucumis melo])

HSP 1 Score: 770.4 bits (1988), Expect = 9.3e-219
Identity = 395/480 (82.29%), Postives = 413/480 (86.04%), Query Frame = 0

Query: 7   VLSRSSCKVMGGKSSKPGSSRRHSSSWSNSQQWSNYNYPGSPYNQPRPQYQFAPPTPSYG 66
           V+S SSC+VMGGKSSK GSSRR+SSS SNSQQW +Y YP SPY Q R   ++APPTPSYG
Sbjct: 28  VVSFSSCEVMGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRYAPPTPSYG 87

Query: 67  GTQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSF 126
           GTQAPETRKRLERKYSRIDDNYNSL+QVTEALARAGLESSNLIVGIDFTKSNEWTGARSF
Sbjct: 88  GTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSF 147

Query: 127 NRRSLHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVC 186
           NRRSLHHIGDEQNPYEQAIAIIG+TLSSFDEDN+IPCFGFGDASTHDQEVFSFY DER C
Sbjct: 148 NRRSLHHIGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFC 207

Query: 187 IGFEEVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDT 246
            GFEEVL RY+ELVPHLRLAGPTSFAPIIEM ITIVEQSGGQYHVLVIIADGQVTRSVDT
Sbjct: 208 NGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDT 267

Query: 247 EGGQFSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLL 306
           E GQFSPQEKKTIEAIVKAS YPLSIILVGVGDGPWDMMREFDDNIPARAFDNF      
Sbjct: 268 ERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNF------ 327

Query: 307 RRVLVFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQ 366
                                      QFVNFTNIMLKN+DR+RKE EFALAALMEIPSQ
Sbjct: 328 ---------------------------QFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQ 387

Query: 367 YKATLELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVA 426
           YKATLEL +LG+        SRGK IDR PLPPP YGPS QKTSNSSSSRP PPSF+GVA
Sbjct: 388 YKATLELGLLGV--------SRGKAIDRIPLPPPFYGPSIQKTSNSSSSRPTPPSFSGVA 447

Query: 427 PVRSAPPLSSVPDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIETKIKLY 486
           PVR+APPLSSV DAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFI+T+IKLY
Sbjct: 448 PVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 466

BLAST of CmaCh10G000870.1 vs. TAIR 10
Match: AT5G14420.1 (RING domain ligase2 )

HSP 1 Score: 547.4 bits (1409), Expect = 1.2e-155
Identity = 302/505 (59.80%), Postives = 351/505 (69.50%), Query Frame = 0

Query: 9   SRSSCKVMGGKSSKPGSSRRHSSSWSNSQQW-----SNYNYPGSP-YNQPRPQYQFAPP- 68
           S+ + +    +S+   S+   SSSW++ Q +      +YNYP  P Y QP P+Y   PP 
Sbjct: 6   SKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQP-PEYTQPPPP 65

Query: 69  --------TPSYGG----TQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLI 128
                    PSY      +   + +KRLERKYS+I D+Y+SLEQVTEALARAGLESSNLI
Sbjct: 66  LYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLI 125

Query: 129 VGIDFTKSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDA 188
           VGIDFTKSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDNLIPC+GFGDA
Sbjct: 126 VGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDA 185

Query: 189 STHDQEVFSFYPDERVCIGFEEVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQY 248
           STHDQ+VFSF  ++R C GFEEVL RY+E+VP L+LAGPTSFAPII+M +TIVEQSGGQY
Sbjct: 186 STHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQY 245

Query: 249 HVLVIIADGQVTRSVDTEGGQFSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFD 308
           HVLVIIADGQVTRSVDTE GQ SPQE+KT++AIV+AS  PLSI+LVGVGDGPWDMMREFD
Sbjct: 246 HVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFD 305

Query: 309 DNIPARAFDNFQVTTLLRRVLVFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRA 368
           DNIPARAFDNF                                 QFVNFT IM KN  ++
Sbjct: 306 DNIPARAFDNF---------------------------------QFVNFTEIMAKNKAQS 365

Query: 369 RKETEFALAALMEIPSQYKATLELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKT 428
            KETEFAL+ALMEIP QYKAT+EL++LG           G   +RFPLPPP  G S    
Sbjct: 366 LKETEFALSALMEIPQQYKATIELNLLG--------RRNGYIPERFPLPPPMRGGSSSYN 425

Query: 429 SNSSSSRP-----MPPSFAGVAPVRSA---PPLSSVPDAHACPICISNAKDMAFGCGHQT 487
           S   S  P     +PP       VRS+   PP SS  D   CPIC+SN KDMAFGCGHQT
Sbjct: 426 SPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLSNPKDMAFGCGHQT 468

BLAST of CmaCh10G000870.1 vs. TAIR 10
Match: AT5G14420.3 (RING domain ligase2 )

HSP 1 Score: 547.4 bits (1409), Expect = 1.2e-155
Identity = 302/505 (59.80%), Postives = 351/505 (69.50%), Query Frame = 0

Query: 9   SRSSCKVMGGKSSKPGSSRRHSSSWSNSQQW-----SNYNYPGSP-YNQPRPQYQFAPP- 68
           S+ + +    +S+   S+   SSSW++ Q +      +YNYP  P Y QP P+Y   PP 
Sbjct: 6   SKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQP-PEYTQPPPP 65

Query: 69  --------TPSYGG----TQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLI 128
                    PSY      +   + +KRLERKYS+I D+Y+SLEQVTEALARAGLESSNLI
Sbjct: 66  LYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLI 125

Query: 129 VGIDFTKSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDA 188
           VGIDFTKSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDNLIPC+GFGDA
Sbjct: 126 VGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDA 185

Query: 189 STHDQEVFSFYPDERVCIGFEEVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQY 248
           STHDQ+VFSF  ++R C GFEEVL RY+E+VP L+LAGPTSFAPII+M +TIVEQSGGQY
Sbjct: 186 STHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQY 245

Query: 249 HVLVIIADGQVTRSVDTEGGQFSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFD 308
           HVLVIIADGQVTRSVDTE GQ SPQE+KT++AIV+AS  PLSI+LVGVGDGPWDMMREFD
Sbjct: 246 HVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFD 305

Query: 309 DNIPARAFDNFQVTTLLRRVLVFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRA 368
           DNIPARAFDNF                                 QFVNFT IM KN  ++
Sbjct: 306 DNIPARAFDNF---------------------------------QFVNFTEIMAKNKAQS 365

Query: 369 RKETEFALAALMEIPSQYKATLELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKT 428
            KETEFAL+ALMEIP QYKAT+EL++LG           G   +RFPLPPP  G S    
Sbjct: 366 LKETEFALSALMEIPQQYKATIELNLLG--------RRNGYIPERFPLPPPMRGGSSSYN 425

Query: 429 SNSSSSRP-----MPPSFAGVAPVRSA---PPLSSVPDAHACPICISNAKDMAFGCGHQT 487
           S   S  P     +PP       VRS+   PP SS  D   CPIC+SN KDMAFGCGHQT
Sbjct: 426 SPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLSNPKDMAFGCGHQT 468

BLAST of CmaCh10G000870.1 vs. TAIR 10
Match: AT5G14420.4 (RING domain ligase2 )

HSP 1 Score: 547.4 bits (1409), Expect = 1.2e-155
Identity = 302/505 (59.80%), Postives = 351/505 (69.50%), Query Frame = 0

Query: 9   SRSSCKVMGGKSSKPGSSRRHSSSWSNSQQW-----SNYNYPGSP-YNQPRPQYQFAPP- 68
           S+ + +    +S+   S+   SSSW++ Q +      +YNYP  P Y QP P+Y   PP 
Sbjct: 6   SKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQP-PEYTQPPPP 65

Query: 69  --------TPSYGG----TQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLI 128
                    PSY      +   + +KRLERKYS+I D+Y+SLEQVTEALARAGLESSNLI
Sbjct: 66  LYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLI 125

Query: 129 VGIDFTKSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDA 188
           VGIDFTKSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDNLIPC+GFGDA
Sbjct: 126 VGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDA 185

Query: 189 STHDQEVFSFYPDERVCIGFEEVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQY 248
           STHDQ+VFSF  ++R C GFEEVL RY+E+VP L+LAGPTSFAPII+M +TIVEQSGGQY
Sbjct: 186 STHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQY 245

Query: 249 HVLVIIADGQVTRSVDTEGGQFSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFD 308
           HVLVIIADGQVTRSVDTE GQ SPQE+KT++AIV+AS  PLSI+LVGVGDGPWDMMREFD
Sbjct: 246 HVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFD 305

Query: 309 DNIPARAFDNFQVTTLLRRVLVFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRA 368
           DNIPARAFDNF                                 QFVNFT IM KN  ++
Sbjct: 306 DNIPARAFDNF---------------------------------QFVNFTEIMAKNKAQS 365

Query: 369 RKETEFALAALMEIPSQYKATLELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKT 428
            KETEFAL+ALMEIP QYKAT+EL++LG           G   +RFPLPPP  G S    
Sbjct: 366 LKETEFALSALMEIPQQYKATIELNLLG--------RRNGYIPERFPLPPPMRGGSSSYN 425

Query: 429 SNSSSSRP-----MPPSFAGVAPVRSA---PPLSSVPDAHACPICISNAKDMAFGCGHQT 487
           S   S  P     +PP       VRS+   PP SS  D   CPIC+SN KDMAFGCGHQT
Sbjct: 426 SPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLSNPKDMAFGCGHQT 468

BLAST of CmaCh10G000870.1 vs. TAIR 10
Match: AT5G14420.2 (RING domain ligase2 )

HSP 1 Score: 547.4 bits (1409), Expect = 1.2e-155
Identity = 302/505 (59.80%), Postives = 351/505 (69.50%), Query Frame = 0

Query: 9   SRSSCKVMGGKSSKPGSSRRHSSSWSNSQQW-----SNYNYPGSP-YNQPRPQYQFAPP- 68
           S+ + +    +S+   S+   SSSW++ Q +      +YNYP  P Y QP P+Y   PP 
Sbjct: 6   SKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQP-PEYTQPPPP 65

Query: 69  --------TPSYGG----TQAPETRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLI 128
                    PSY      +   + +KRLERKYS+I D+Y+SLEQVTEALARAGLESSNLI
Sbjct: 66  LYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLI 125

Query: 129 VGIDFTKSNEWTGARSFNRRSLHHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDA 188
           VGIDFTKSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDNLIPC+GFGDA
Sbjct: 126 VGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDA 185

Query: 189 STHDQEVFSFYPDERVCIGFEEVLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQY 248
           STHDQ+VFSF  ++R C GFEEVL RY+E+VP L+LAGPTSFAPII+M +TIVEQSGGQY
Sbjct: 186 STHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQY 245

Query: 249 HVLVIIADGQVTRSVDTEGGQFSPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFD 308
           HVLVIIADGQVTRSVDTE GQ SPQE+KT++AIV+AS  PLSI+LVGVGDGPWDMMREFD
Sbjct: 246 HVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFD 305

Query: 309 DNIPARAFDNFQVTTLLRRVLVFCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRA 368
           DNIPARAFDNF                                 QFVNFT IM KN  ++
Sbjct: 306 DNIPARAFDNF---------------------------------QFVNFTEIMAKNKAQS 365

Query: 369 RKETEFALAALMEIPSQYKATLELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKT 428
            KETEFAL+ALMEIP QYKAT+EL++LG           G   +RFPLPPP  G S    
Sbjct: 366 LKETEFALSALMEIPQQYKATIELNLLG--------RRNGYIPERFPLPPPMRGGSSSYN 425

Query: 429 SNSSSSRP-----MPPSFAGVAPVRSA---PPLSSVPDAHACPICISNAKDMAFGCGHQT 487
           S   S  P     +PP       VRS+   PP SS  D   CPIC+SN KDMAFGCGHQT
Sbjct: 426 SPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLSNPKDMAFGCGHQT 468

BLAST of CmaCh10G000870.1 vs. TAIR 10
Match: AT3G01650.1 (RING domain ligase1 )

HSP 1 Score: 526.9 bits (1356), Expect = 1.7e-149
Identity = 297/536 (55.41%), Postives = 347/536 (64.74%), Query Frame = 0

Query: 16  MGGKSSKPGSSRRHSSSWSNSQQW-----SNYNYP------------------------- 75
           MGG +SK  SS   SSSW++ Q +      +YNYP                         
Sbjct: 1   MGGGNSKEESSSPSSSSWASHQSYPQYGPDSYNYPPPPTYAPAPSPAPAPAPVPAPSPAS 60

Query: 76  ------------GSPYNQPRPQYQFAP-PTPSYGGTQAPE-------------------- 135
                         P N P P Y  AP P  SYG   + E                    
Sbjct: 61  SYGPQYSQEGYASQPNNPPPPTYAPAPSPASSYGHQYSQEGYASAASQPNYPPPPSQSQV 120

Query: 136 -TRKRLERKYSRIDDNYNSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSL 195
             RK+ +R+YS+I DNY+SL QV+EAL RAGLESSNLIVGIDFTKSNEWTGA+SFNR+SL
Sbjct: 121 ADRKKFDRRYSKISDNYSSLLQVSEALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSL 180

Query: 196 HHIGDEQNPYEQAIAIIGKTLSSFDEDNLIPCFGFGDASTHDQEVFSFYPDERVCIGFEE 255
           HH+ +  NPYEQAI IIG+TL++FDEDNLIPC+GFGDASTHDQ+VFSFYP+ R C GFEE
Sbjct: 181 HHLSNTPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEE 240

Query: 256 VLDRYRELVPHLRLAGPTSFAPIIEMGITIVEQSGGQYHVLVIIADGQVTRSVDTEGGQF 315
           VL RYRE+VP L+LAGPTSFAPIIEM +T+VEQS GQYHVLVIIADGQVTRSVDTE G+ 
Sbjct: 241 VLARYREIVPQLKLAGPTSFAPIIEMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRL 300

Query: 316 SPQEKKTIEAIVKASAYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQVTTLLRRVLV 375
           SPQE+KT++AIVKAS  PLSI+LVGVGDGPWDMM+EFDDNIPARAFDNF           
Sbjct: 301 SPQEQKTVDAIVKASTLPLSIVLVGVGDGPWDMMQEFDDNIPARAFDNF----------- 360

Query: 376 FCFSNWTCFCHSLKSEKFHFALQFVNFTNIMLKNMDRARKETEFALAALMEIPSQYKATL 435
                                 QFVNFT IM KN D++RKETEFAL+ALMEIP QYKAT+
Sbjct: 361 ----------------------QFVNFTEIMSKNKDQSRKETEFALSALMEIPPQYKATI 420

Query: 436 ELDILGILFHYSTSASRGKEIDRFPLPPPRYGPSFQKTSNSSSSRPMPPSFAGVAPVR-S 487
           EL++LG+          G    R PLPPP      Q  S+ SSSR +P     V P    
Sbjct: 421 ELNLLGV--------RNGNIPQRIPLPPP-----VQSGSSFSSSR-IPNFEPSVPPYPFE 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LY871.7e-15459.80E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 ... [more]
Q9SS902.4e-14855.41E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 ... [more]
Q8LB882.4e-14058.63E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 ... [more]
Q8RX268.4e-11454.81E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 ... [more]
Q9SAL04.8e-10948.59E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1J9U11.3e-24291.30E3 ubiquitin-protein ligase RGLG2-like OS=Cucurbita maxima OX=3661 GN=LOC1114848... [more]
A0A6J1E1J11.1e-23890.23E3 ubiquitin-protein ligase RGLG2-like OS=Cucurbita moschata OX=3662 GN=LOC11142... [more]
A0A1S4E4Q64.5e-21982.29E3 ubiquitin-protein ligase RGLG2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A1S4E4Q22.9e-21882.56E3 ubiquitin-protein ligase RGLG2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A1S4E5G02.9e-21882.56E3 ubiquitin-protein ligase RGLG2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_022987237.12.7e-24291.30E3 ubiquitin-protein ligase RGLG2-like [Cucurbita maxima][more]
KAG6589412.12.1e-23989.92E3 ubiquitin-protein ligase RGLG2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023515574.12.8e-23990.66E3 ubiquitin-protein ligase RGLG2-like [Cucurbita pepo subsp. pepo][more]
XP_022921789.12.4e-23890.23E3 ubiquitin-protein ligase RGLG2-like [Cucurbita moschata][more]
XP_016903209.19.3e-21982.29PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G14420.11.2e-15559.80RING domain ligase2 [more]
AT5G14420.31.2e-15559.80RING domain ligase2 [more]
AT5G14420.41.2e-15559.80RING domain ligase2 [more]
AT5G14420.21.2e-15559.80RING domain ligase2 [more]
AT3G01650.11.7e-14955.41RING domain ligase1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 74..94
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 441..480
e-value: 1.4E-6
score: 28.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..53
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 404..419
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 391..422
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..74
NoneNo IPR availablePANTHERPTHR45751COPINE FAMILY PROTEIN 1coord: 16..302
coord: 332..486
NoneNo IPR availablePANTHERPTHR45751:SF29RING-FINGER PROTEINcoord: 16..302
coord: 332..486
NoneNo IPR availableCDDcd16729RING-HC_RGLG_plantcoord: 443..486
e-value: 2.32188E-23
score: 90.5759
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 441..482
IPR002035von Willebrand factor, type ASMARTSM00327VWA_4coord: 105..310
e-value: 1.8E-6
score: 37.5
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 430..486
e-value: 3.2E-10
score: 41.3
IPR010734Copine, C-terminalPFAMPF07002Copinecoord: 333..375
e-value: 9.0E-6
score: 25.3
coord: 130..301
e-value: 2.4E-60
score: 203.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 443..476
score: 10.792683
IPR036465von Willebrand factor A-like domain superfamilySUPERFAMILY53300vWA-likecoord: 107..289

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh10G000870CmaCh10G000870gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh10G000870.1:exon:689CmaCh10G000870.1:exon:689exon
CmaCh10G000870.1:exon:688CmaCh10G000870.1:exon:688exon
CmaCh10G000870.1:exon:687CmaCh10G000870.1:exon:687exon
CmaCh10G000870.1:exon:686CmaCh10G000870.1:exon:686exon
CmaCh10G000870.1:exon:685CmaCh10G000870.1:exon:685exon
CmaCh10G000870.1:exon:684CmaCh10G000870.1:exon:684exon
CmaCh10G000870.1:exon:683CmaCh10G000870.1:exon:683exon
CmaCh10G000870.1:exon:682CmaCh10G000870.1:exon:682exon
CmaCh10G000870.1:exon:681CmaCh10G000870.1:exon:681exon
CmaCh10G000870.1:exon:680CmaCh10G000870.1:exon:680exon
CmaCh10G000870.1:exon:679CmaCh10G000870.1:exon:679exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh10G000870.1:cdsCmaCh10G000870.1:cds_11CDS
CmaCh10G000870.1:cdsCmaCh10G000870.1:cds_10CDS
CmaCh10G000870.1:cdsCmaCh10G000870.1:cds_9CDS
CmaCh10G000870.1:cdsCmaCh10G000870.1:cds_8CDS
CmaCh10G000870.1:cdsCmaCh10G000870.1:cds_7CDS
CmaCh10G000870.1:cdsCmaCh10G000870.1:cds_6CDS
CmaCh10G000870.1:cdsCmaCh10G000870.1:cds_5CDS
CmaCh10G000870.1:cdsCmaCh10G000870.1:cds_4CDS
CmaCh10G000870.1:cdsCmaCh10G000870.1:cds_3CDS
CmaCh10G000870.1:cdsCmaCh10G000870.1:cds_2CDS
CmaCh10G000870.1:cdsCmaCh10G000870.1:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh10G000870.1CmaCh10G000870.1-proteinpolypeptide