CmaCh04G025110.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G025110.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCationic amino acid transporter 6
LocationCma_Chr04: 17207963 .. 17211988 (-)
Sequence length1868
RNA-Seq ExpressionCmaCh04G025110.1
SyntenyCmaCh04G025110.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCGCCATGGACGCCCATTCAGCTTCTTTTTCAAGCTTTTCCGCATACCTTCGAGCTCTCTCTCTCACTCCTTCTCGTCTTTCTCGACGGGCCTTCTCCGTCTCCACCTCCTTCGATGAAATGACTTCTACACGATCCACTTCCGGCGTTCATATGCATAGGACTCTCCGCTGGTTCGACCTTGTCGGCTTCGGCCTTGGCGGTATGATCGGCGCTGGCGTCTTCGTCACAACCGGTCCGGCTGCTCACCAGGCCGGTCCTGCCATTGTTCTGTCTTACGCCATTGCTGGCCTCTGTGCGCTCTTATCGGCTTTCTGCTACACCGAGTTCGCCGTCGATATGCCTGTAGCCGGTGGCGCCTTTAGTTATCTACGTATCACATTCGGTGTGGCTTCCTGATTCTATTTCTATTTCAAATGTGTATGTTTAATTTGATCATTTCCGTGCTTCTTCTCACTCGGAGATTTTTGTTTGTTTGTTTTTGTGTACAGGTGAATTCGCTGGATTTTTGACCGGTGCGAATCTGATAATGGACTATGTGATGTCCAACGCTGCTGTCGCTAGGAGTTTCACTCAATACCTGGGCGCCGCAATCGGTATCTCCACCGCTAAATGGAGACTCGTCGTTCCCGGACTCCCCAAGGGATTCGATCAAATCGATTTGATTGCCGTCGCCGTTGTTTTGATCATCACAATCATCATCTGTTACAGGTCTGTGCGAAATCGAAAATCATGAATCGTGTTCGAATTCGTTGTGAAACTAAATAAGGCTAGTTTTATTTGCGGATGTTCTGTTTTTCAGTACGAAACAGAGCTCGGTGGTGAACATGATGCTGACAGCAATACACATTCTATTCATAGCTTTTCTGATCGTATTCGGATTTTGGAAAGGCGATTGGAAGAACTTCACCGAACCAGGCGATCCGAAGAACGAATCTGGATTCTTCCCTCACGGAGCCACAGGCGTTTTCAAAGGCGCGTCTCTGGTTTCCCTCAGTTACATCGGCTACGACGCTGTATCGACGATGGCGGAGGAGGTTCGTAATCCGGCAAAGGACATCCCAGTCGGCGTCTCCGGCTCCGTCGTTCTCGTCACCATCCTGTACTGCCTAATGGCGGCTTCAATGGCGAAGCTCCTTCCCTACGATATGGTACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCACAAAAATACAAATTCGTACAGTCTGATTTACAGTTGAGGGAAATCGGTGTTGATTACATTGAAACAACTTTGTACGTGGTTGCGGCAGATCAAGGCGGAGGCGCCGCTCGCGGAGGCGTTTGCGAGGTGGAAGTGGGTGTCGAACGTGATCGGGGGCGGAGCAAGTTTCGGGATATTGACGTCGCTGCTGGTGGCGATGATGGGGCAGGCTCGATACATGTGCGTCATTGGACGGTCCAGAGTGGTCCCTGCTTGGTTCGCCGAGGTCCATCCCAAAACCTCCACCCCTCTCAACGCCTCCGCCTTTCTTGGTAACTCATTTTAACCCTACTCACTTTCAACATTTAATCTTTTAAATTACGCCAATGAGCTATTATCATGTATTAAATTATACCAAATATATATTTTCTAGTTTCAATTTTTATACAATTGAACCATCATGACTGGAAAAGAATTTGAATATCTGATTTATTGGTTCAGAATTCATAGATTAATCCGATTAGGTGAGCTATGCAAATTGACATCTAACCTTTTAACCTTATGAACGAGATTTATTTTTCTTGTTCGCGAATGCTCTTCCTTTCAACGTTGAAGGGGGCCTTGAGAATCTGCTGTTCCAAATGGGAGTGTTTGGATAGAGGGAAAGAAATCTTTTGGGGCCATGGTTTTGGTGGAACTAGAAGGAGACCTTTTTTATATTTGATTTATTAATTATCATGATAATTATTTGAAAGAAAAAAAAAAAAGTGGGACCTCACCACTTATAGCATGAAATCAATGGCTAAAACCCAAGACAGTGAGAGAGATAGAGAGGGTTTTGGTGAAGTAAACTCTTGCATTAGTTTGTGCCTTGTGGTTCATAATGGTTTAGAGCAATAGATATTGTTAAGATGGTGGGAGAGAGTTTTTAAACTTTTATAGGATAGCAACACTTTTGTCATGGCTTCACAGGCATCTTCTGTGTTTCGAGGAAAAATCAAGGAATTTGTATATAAAACACTTGGCGGTCTTCTTGTTTCAACACCATGGAAAATGAAAGATACAGCCTTTTCTAACTGTTTTCTTGCACTTTTCTTGTGTTTCGTTCTTAAGGGAAATTAAAAATAAAAACAACTACAAGGAGGTGGGTTGCTTTCATGTAACCGTTCAAGCCCACCGCTTCAAGTCCACTGCTAGTAGATATTATCTTTTCGGGCTTCCCCTTTCAAACTTCTCCTTTTATTAAAATGCTACTGATAGGGATGGTCTCGTATGAATCATGGGATGTCCTGTATTGTTGTGTTGCGTCGACCGTGTGATGGTCTCGTATGAATCTTTTGTAGAGTTTCTTCTTCCCTTTTGATTTATGTTGAGCCTTAACACAAGACAAGGTTTTCACAAGCTGTGTGTAAATATTTACACGGTAAGAGATAGGTGGTCTAGATTGGCTCAACTCAACTGCAGGATATATCTCTTTGGTTTAACCCAATTTTGAAAATGACCGATCATATTTCGATAAGAATCAAGTTTCTTCATCTATGCTGTCACTTTATCCACTACAATTATTGAGACTCTGCACAAATTCCTTAATTTCCTGTTGTTTAAATCAATTTTTATTGGTTTGATTCAGGGATTTTTACGGCTGTGATCGCTCTTTTCGTTGACCTTGATGTCCTACTCAACTTTGTATGCATTGGTACACTCTTTGTGTTCTACATGGTCGCCAATGCTATTATTTTCCGACGTTATGTGGTGATAGGGAGCACCAAGCCACAACCAACGCTATCATTCCTCTGCTCATTCTCACTCACCGCTATTATATTCACTCTCATATGGCACTTTGTAACTCCAGGGAGGGCCAAAACTGCCCTACTCACCGTCACTGCCATCGTCGCCATCGTTGTCTCGCTAATATTCAATTGCGTGGTACCTCAAGCACGGAAACCTGAATTCTGGGGCGTCCCCTTGATGCCATGGATACCTTGTGCATCAATCTTCCTAAACTTGTTCTTGTTGGGGTCCCTCGATGGCACGTCCTATGTAAGATTCGTGTTCTTTTCGATCCTCGTCGTGCTTGTTTATGTGTTGTATGGTGTCCATTCAAGCTCAGATGCTGAAGGAGACGACTCCCTATTCGTAAAAAATGGGGAGATTCCAGAATTACAAAACAACTTCAAAGTATAGTTTATGTGATTGTTTCTTTTACCCGACAAATTAAAACCTTATTGTATAAAGTGTCGAAGAGAGGTGTTCCAAAAGTGGAAAGCTATAAATATAGTTTATGGGAACTAAAATGATTTTTTTAGTTCAACGAGTGGGAC

mRNA sequence

TCCGCCATGGACGCCCATTCAGCTTCTTTTTCAAGCTTTTCCGCATACCTTCGAGCTCTCTCTCTCACTCCTTCTCGTCTTTCTCGACGGGCCTTCTCCGTCTCCACCTCCTTCGATGAAATGACTTCTACACGATCCACTTCCGGCGTTCATATGCATAGGACTCTCCGCTGGTTCGACCTTGTCGGCTTCGGCCTTGGCGGTATGATCGGCGCTGGCGTCTTCGTCACAACCGGTCCGGCTGCTCACCAGGCCGGTCCTGCCATTGTTCTGTCTTACGCCATTGCTGGCCTCTGTGCGCTCTTATCGGCTTTCTGCTACACCGAGTTCGCCGTCGATATGCCTGTAGCCGGTGGCGCCTTTAGTTATCTACGTATCACATTCGGTGAATTCGCTGGATTTTTGACCGGTGCGAATCTGATAATGGACTATGTGATGTCCAACGCTGCTGTCGCTAGGAGTTTCACTCAATACCTGGGCGCCGCAATCGGTATCTCCACCGCTAAATGGAGACTCGTCGTTCCCGGACTCCCCAAGGGATTCGATCAAATCGATTTGATTGCCGTCGCCGTTGTTTTGATCATCACAATCATCATCTGTTACAGTACGAAACAGAGCTCGGTGGTGAACATGATGCTGACAGCAATACACATTCTATTCATAGCTTTTCTGATCGTATTCGGATTTTGGAAAGGCGATTGGAAGAACTTCACCGAACCAGGCGATCCGAAGAACGAATCTGGATTCTTCCCTCACGGAGCCACAGGCGTTTTCAAAGGCGCGTCTCTGGTTTCCCTCAGTTACATCGGCTACGACGCTGTATCGACGATGGCGGAGGAGGTTCGTAATCCGGCAAAGGACATCCCAGTCGGCGTCTCCGGCTCCGTCGTTCTCGTCACCATCCTGTACTGCCTAATGGCGGCTTCAATGGCGAAGCTCCTTCCCTACGATATGATCAAGGCGGAGGCGCCGCTCGCGGAGGCGTTTGCGAGGTGGAAGTGGGTGTCGAACGTGATCGGGGGCGGAGCAAGTTTCGGGATATTGACGTCGCTGCTGGTGGCGATGATGGGGCAGGCTCGATACATGTGCGTCATTGGACGGTCCAGAGTGGTCCCTGCTTGGTTCGCCGAGGTCCATCCCAAAACCTCCACCCCTCTCAACGCCTCCGCCTTTCTTGGGATTTTTACGGCTGTGATCGCTCTTTTCGTTGACCTTGATGTCCTACTCAACTTTGTATGCATTGGTACACTCTTTGTGTTCTACATGGTCGCCAATGCTATTATTTTCCGACGTTATGTGGTGATAGGGAGCACCAAGCCACAACCAACGCTATCATTCCTCTGCTCATTCTCACTCACCGCTATTATATTCACTCTCATATGGCACTTTGTAACTCCAGGGAGGGCCAAAACTGCCCTACTCACCGTCACTGCCATCGTCGCCATCGTTGTCTCGCTAATATTCAATTGCGTGGTACCTCAAGCACGGAAACCTGAATTCTGGGGCGTCCCCTTGATGCCATGGATACCTTGTGCATCAATCTTCCTAAACTTGTTCTTGTTGGGGTCCCTCGATGGCACGTCCTATGTAAGATTCGTGTTCTTTTCGATCCTCGTCGTGCTTGTTTATGTGTTGTATGGTGTCCATTCAAGCTCAGATGCTGAAGGAGACGACTCCCTATTCGTAAAAAATGGGGAGATTCCAGAATTACAAAACAACTTCAAAGTATAGTTTATGTGATTGTTTCTTTTACCCGACAAATTAAAACCTTATTGTATAAAGTGTCGAAGAGAGGTGTTCCAAAAGTGGAAAGCTATAAATATAGTTTATGGGAACTAAAATGATTTTTTTAGTTCAACGAGTGGGAC

Coding sequence (CDS)

ATGGACGCCCATTCAGCTTCTTTTTCAAGCTTTTCCGCATACCTTCGAGCTCTCTCTCTCACTCCTTCTCGTCTTTCTCGACGGGCCTTCTCCGTCTCCACCTCCTTCGATGAAATGACTTCTACACGATCCACTTCCGGCGTTCATATGCATAGGACTCTCCGCTGGTTCGACCTTGTCGGCTTCGGCCTTGGCGGTATGATCGGCGCTGGCGTCTTCGTCACAACCGGTCCGGCTGCTCACCAGGCCGGTCCTGCCATTGTTCTGTCTTACGCCATTGCTGGCCTCTGTGCGCTCTTATCGGCTTTCTGCTACACCGAGTTCGCCGTCGATATGCCTGTAGCCGGTGGCGCCTTTAGTTATCTACGTATCACATTCGGTGAATTCGCTGGATTTTTGACCGGTGCGAATCTGATAATGGACTATGTGATGTCCAACGCTGCTGTCGCTAGGAGTTTCACTCAATACCTGGGCGCCGCAATCGGTATCTCCACCGCTAAATGGAGACTCGTCGTTCCCGGACTCCCCAAGGGATTCGATCAAATCGATTTGATTGCCGTCGCCGTTGTTTTGATCATCACAATCATCATCTGTTACAGTACGAAACAGAGCTCGGTGGTGAACATGATGCTGACAGCAATACACATTCTATTCATAGCTTTTCTGATCGTATTCGGATTTTGGAAAGGCGATTGGAAGAACTTCACCGAACCAGGCGATCCGAAGAACGAATCTGGATTCTTCCCTCACGGAGCCACAGGCGTTTTCAAAGGCGCGTCTCTGGTTTCCCTCAGTTACATCGGCTACGACGCTGTATCGACGATGGCGGAGGAGGTTCGTAATCCGGCAAAGGACATCCCAGTCGGCGTCTCCGGCTCCGTCGTTCTCGTCACCATCCTGTACTGCCTAATGGCGGCTTCAATGGCGAAGCTCCTTCCCTACGATATGATCAAGGCGGAGGCGCCGCTCGCGGAGGCGTTTGCGAGGTGGAAGTGGGTGTCGAACGTGATCGGGGGCGGAGCAAGTTTCGGGATATTGACGTCGCTGCTGGTGGCGATGATGGGGCAGGCTCGATACATGTGCGTCATTGGACGGTCCAGAGTGGTCCCTGCTTGGTTCGCCGAGGTCCATCCCAAAACCTCCACCCCTCTCAACGCCTCCGCCTTTCTTGGGATTTTTACGGCTGTGATCGCTCTTTTCGTTGACCTTGATGTCCTACTCAACTTTGTATGCATTGGTACACTCTTTGTGTTCTACATGGTCGCCAATGCTATTATTTTCCGACGTTATGTGGTGATAGGGAGCACCAAGCCACAACCAACGCTATCATTCCTCTGCTCATTCTCACTCACCGCTATTATATTCACTCTCATATGGCACTTTGTAACTCCAGGGAGGGCCAAAACTGCCCTACTCACCGTCACTGCCATCGTCGCCATCGTTGTCTCGCTAATATTCAATTGCGTGGTACCTCAAGCACGGAAACCTGAATTCTGGGGCGTCCCCTTGATGCCATGGATACCTTGTGCATCAATCTTCCTAAACTTGTTCTTGTTGGGGTCCCTCGATGGCACGTCCTATGTAAGATTCGTGTTCTTTTCGATCCTCGTCGTGCTTGTTTATGTGTTGTATGGTGTCCATTCAAGCTCAGATGCTGAAGGAGACGACTCCCTATTCGTAAAAAATGGGGAGATTCCAGAATTACAAAACAACTTCAAAGTATAG

Protein sequence

MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYMCVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMVANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAIVVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLVYVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV
Homology
BLAST of CmaCh04G025110.1 vs. ExPASy Swiss-Prot
Match: Q9LZ20 (Cationic amino acid transporter 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT6 PE=2 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 4.5e-223
Identity = 400/564 (70.92%), Postives = 465/564 (82.45%), Query Frame = 0

Query: 6   ASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLG 65
           +SFSS   YL +LS TPSRLSRRA SVSTS DEM+  R+ SG  M RTLRW+DL+G G+G
Sbjct: 13  SSFSSLRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIG 72

Query: 66  GMIGAGVFVTTGPAAH-QAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRI 125
           GM+GAGVFVTTG A+   AGP+IV+SYAIAGLCALLSAFCYTEFAV +PVAGGAFSY+RI
Sbjct: 73  GMVGAGVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRI 132

Query: 126 TFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDL 185
           TFGEF  F TGANL+MDYVMSNAAV+RSFT YLG A GIST+KWR VV GLPKGF++ID 
Sbjct: 133 TFGEFPAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFGISTSKWRFVVSGLPKGFNEIDP 192

Query: 186 IAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNE 245
           +AV VVL+IT+IIC ST++SS VNM++TA HI FI F+IV GF KGD KN + P +P++ 
Sbjct: 193 VAVLVVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHP 252

Query: 246 SGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLM 305
           SGFFP GA GVF GA++V LSYIGYDAVSTMAEEV NP KDIPVGVSGSV +VT+LYCLM
Sbjct: 253 SGFFPFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLM 312

Query: 306 AASMAKLLPYDMIKAEAPLAEAFA---RWKWVSNVIGGGASFGILTSLLVAMMGQARYMC 365
           A SM+ LLPYD+I  EAP + AF     W+WV+ V+G GASFGILTSLLVAM+GQARYMC
Sbjct: 313 AVSMSMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMC 372

Query: 366 VIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMV 425
           VIGRSRVVP WFA++HPKTSTP+NAS FLGIFTA +ALF DL+VLLN V IGTLFVFYMV
Sbjct: 373 VIGRSRVVPFWFAKIHPKTSTPVNASTFLGIFTAALALFTDLNVLLNLVSIGTLFVFYMV 432

Query: 426 ANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAIV 485
           ANA+IFRRYV +G TKP PTL FL  FS+T+++FTLIW  V  G+ K  +L  +A+VAI 
Sbjct: 433 ANALIFRRYVPVGPTKPWPTLCFLTLFSITSLVFTLIWKLVPEGKPKAFMLGASAVVAIA 492

Query: 486 VSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLVY 545
           + L F CVVPQARKPE WGVP MPW PC SIFLN+FLLGSLD  SYVRF FFS L+VLVY
Sbjct: 493 IVLSFQCVVPQARKPELWGVPFMPWTPCVSIFLNIFLLGSLDAPSYVRFGFFSGLIVLVY 552

Query: 546 VLYGVHSSSDAEGDDSLFVKNGEI 566
           + YGVH+SSDAE + S  VK+G++
Sbjct: 553 LFYGVHASSDAEANGSFGVKDGQV 576

BLAST of CmaCh04G025110.1 vs. ExPASy Swiss-Prot
Match: Q9SQZ0 (Cationic amino acid transporter 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT7 PE=3 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 5.0e-222
Identity = 399/568 (70.25%), Postives = 468/568 (82.39%), Query Frame = 0

Query: 7   SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLGG 66
           SFSS   YL +LS TPSR SRRA SVSTS+DEM+  R+ SG  M RTLRW+DL+G G+GG
Sbjct: 13  SFSSLRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 72

Query: 67  MIGAGVFVTTGPAAH-QAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRIT 126
           MIGAGVFVTTG A+   AGP+IV+SYAIAGLCALLSAFCYTEFAV +PVAGGAFSY+RIT
Sbjct: 73  MIGAGVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 132

Query: 127 FGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDLI 186
           FGEF  F+TGANLIMDYV+SNAAV+R FT YLG+A GIST++WR +V GLP GF++ID I
Sbjct: 133 FGEFPAFITGANLIMDYVLSNAAVSRGFTAYLGSAFGISTSEWRFIVSGLPNGFNEIDPI 192

Query: 187 AVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNES 246
           AV VVL +T +ICYST++SS VNM+LTA+HI FI F+IV GF KGD KN T P +P+N S
Sbjct: 193 AVIVVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPENPS 252

Query: 247 GFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLMA 306
           GFFP G +GVF GA++V LSYIGYDAVSTMAEEV++P KDIP+G+SGSV +V +LYCLMA
Sbjct: 253 GFFPFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCLMA 312

Query: 307 ASMAKLLPYDMIKAEAPLAEAFAR---WKWVSNVIGGGASFGILTSLLVAMMGQARYMCV 366
            SM+ LLPYD+I AEAP + AF++   W+WV+ V+G GASFGILTSL+VAM+GQARYMCV
Sbjct: 313 ISMSMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYMCV 372

Query: 367 IGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMVA 426
           IGRSRVVP WFA+VHPKTSTP+NASAFLGIFTAV+ALF DL+VLLN V IGTLFVFYMVA
Sbjct: 373 IGRSRVVPIWFAKVHPKTSTPVNASAFLGIFTAVLALFTDLNVLLNLVSIGTLFVFYMVA 432

Query: 427 NAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAIVV 486
           NA+IFRRYV +G T+P PTLSFLC FS+T+I+FTL+W     G  K  +L  + + AI +
Sbjct: 433 NAVIFRRYVTVGYTEPWPTLSFLCLFSITSILFTLVWQLAPSGPPKWFILGASTVTAIAI 492

Query: 487 SLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLVYV 546
             IF+CVVPQAR PEFWGVPLMPW PC SIFLN+FLLGSLD  SY+RF FFS LVVLVYV
Sbjct: 493 VQIFHCVVPQARIPEFWGVPLMPWTPCVSIFLNIFLLGSLDAPSYIRFGFFSGLVVLVYV 552

Query: 547 LYGVHSSSDAEGDDSLFVKNGEIPELQN 571
            Y VH+S DAEGD SL  K+ E  E  N
Sbjct: 553 FYSVHASYDAEGDGSLDFKDVESLERIN 580

BLAST of CmaCh04G025110.1 vs. ExPASy Swiss-Prot
Match: Q84MA5 (Cationic amino acid transporter 1 OS=Arabidopsis thaliana OX=3702 GN=CAT1 PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.3e-116
Identity = 232/548 (42.34%), Postives = 343/548 (62.59%), Query Frame = 0

Query: 7   SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLGG 66
           SF S   YL+AL  TPSR   R  + S   DE+   ++ SG  M +TL W+DL+ FG+G 
Sbjct: 27  SFQSMGNYLKALKETPSRFMDRIMTRSLDSDEINEMKARSGHEMKKTLTWWDLMWFGIGA 86

Query: 67  MIGAGVFVTTG-PAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRIT 126
           +IG+G+FV TG  A + +GPA+VLSY ++G+ A+LS FCYTEFAV++PVAGG+F+YLR+ 
Sbjct: 87  VIGSGIFVLTGLEARNHSGPAVVLSYVVSGVSAMLSVFCYTEFAVEIPVAGGSFAYLRVE 146

Query: 127 FGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDLI 186
            G+F  F+   N+I++YV+  AAVARS+T Y    +      +R++V  L + +  +D I
Sbjct: 147 LGDFMAFIAAGNIILEYVVGGAAVARSWTSYFATLLNHKPEDFRIIVHKLGEDYSHLDPI 206

Query: 187 AVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNES 246
           AV V  II ++    TK SS  N + + IH++ I F+I+ GF K D KN+         S
Sbjct: 207 AVGVCAIICVLAVVGTKGSSRFNYIASIIHMVVILFVIIAGFTKADVKNY---------S 266

Query: 247 GFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLMA 306
            F P+G  GVFK A+++  +YIG+DAVSTMAEE +NP +DIP+G+ GS+V+ T+ YCLMA
Sbjct: 267 DFTPYGVRGVFKSAAVLFFAYIGFDAVSTMAEETKNPGRDIPIGLVGSMVVTTVCYCLMA 326

Query: 307 ASMAKLLPYDMIKAEAPLAEAFAR--WKWVSNVIGGGASFGILTSLLVAMMGQARYMCVI 366
            ++  + PY  I  +AP + AF+   W W   ++  GA  G+ T LLV  +GQARYM  I
Sbjct: 327 VTLCLMQPYQQIDPDAPFSVAFSAVGWDWAKYIVAFGALKGMTTVLLVGAIGQARYMTHI 386

Query: 367 GRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMVAN 426
            R+ ++P W A+V+ KT TP+NA+  +   TA+IA F  L +L + + + TLF+F  VA 
Sbjct: 387 ARAHMMPPWLAQVNAKTGTPINATVVMLAATALIAFFTKLKILADLLSVSTLFIFMFVAV 446

Query: 427 AIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAIVVS 486
           A++ RRY V G T  +    FL    L     T    +            +T  +  + +
Sbjct: 447 ALLVRRYYVTGETSTRDRNKFLVFLGLILASSTATAVYWALEEEGWIGYCITVPIWFLST 506

Query: 487 LIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLVYVL 546
           +    +VPQAR P+ WGVPL+PW+P ASI +N+FLLGS+D  S+VRF  ++ ++++ YVL
Sbjct: 507 VAMKFLVPQARAPKIWGVPLVPWLPSASIAINIFLLGSIDTKSFVRFAIWTGILLIYYVL 565

Query: 547 YGVHSSSD 552
           +G+H++ D
Sbjct: 567 FGLHATYD 565

BLAST of CmaCh04G025110.1 vs. ExPASy Swiss-Prot
Match: O64759 (Cationic amino acid transporter 5 OS=Arabidopsis thaliana OX=3702 GN=CAT5 PE=1 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 2.5e-104
Identity = 233/552 (42.21%), Postives = 336/552 (60.87%), Query Frame = 0

Query: 7   SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLGG 66
           SF SF +Y  ALS T SR   R  S S   +E    +  S   M R L W+DLV FG G 
Sbjct: 21  SFQSFGSYRAALSQTCSRFKNRLVSRSDDENERFELKKQSEHEMKRCLTWWDLVWFGFGS 80

Query: 67  MIGAGVFVTTGPAAH-QAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRIT 126
           +IGAG+FV TG  AH QAGPAIVLSY ++GL A+LS FCYTEFAV++PVAGG+F+YLRI 
Sbjct: 81  VIGAGIFVLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYTEFAVEIPVAGGSFAYLRIE 140

Query: 127 FGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDLI 186
            G+FA F+T  N++++ ++  AAVAR++T Y    +  S    R +   L  GF+ +D I
Sbjct: 141 LGDFAAFITAGNILLESIVGTAAVARAWTSYFATLLNRSPNALR-IKTDLSSGFNLLDPI 200

Query: 187 AVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNES 246
           AV V+     I   ST+++S++N + +AI+ L I F+I+ GF   D  N T         
Sbjct: 201 AVVVIAASATIASISTRKTSLLNWIASAINTLVIFFVIIAGFIHADTSNLTP-------- 260

Query: 247 GFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLMA 306
            F P G  GVF+ A++V  +Y G+D+++TMAEE +NP++DIP+G+ GS+ ++T++YCLMA
Sbjct: 261 -FLPFGPEGVFRAAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMSIITVIYCLMA 320

Query: 307 ASMAKLLPYDMIKAEAPLAEAF--ARWKWVSNVIGGGASFGILTSLLVAMMGQARYMCVI 366
            S++ +  Y  I   A  + AF     KW   ++  GA  G+ T LLV  +GQARY+  I
Sbjct: 321 LSLSMMQKYTDIDPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGALGQARYVTHI 380

Query: 367 GRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMVAN 426
            R+ ++P  FA VHPKT TP+NA+  + I +A+IA F  LDVL + + I TLF+F M+  
Sbjct: 381 ARTHMIPPIFALVHPKTGTPINANLLVAIPSALIAFFSGLDVLASLLSISTLFIFTMMPI 440

Query: 427 AIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTL----IWHFVTPGRAKTALLTVTAIVA 486
           A++ RRY V   T P+  L  L +  L  ++ ++     W     G      +TV     
Sbjct: 441 ALLVRRYYVRQDT-PRVHLIKLITCLLFVVVSSMGTSAYWGMQRKGSWIGYTVTVPFWFL 500

Query: 487 IVVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVL 546
             + ++F   VPQ R P+ WGVPL+PW+PC SI  N+FL+GSL   ++VRF   ++ ++L
Sbjct: 501 GTLGIVF--FVPQQRTPKVWGVPLVPWLPCLSIATNIFLMGSLGAMAFVRFGVCTLAMLL 559

Query: 547 VYVLYGVHSSSD 552
            Y L G+H++ D
Sbjct: 561 YYFLLGLHATFD 559

BLAST of CmaCh04G025110.1 vs. ExPASy Swiss-Prot
Match: Q9SHH0 (Cationic amino acid transporter 8, vacuolar OS=Arabidopsis thaliana OX=3702 GN=CAT8 PE=2 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 9.7e-101
Identity = 219/551 (39.75%), Postives = 335/551 (60.80%), Query Frame = 0

Query: 7   SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLGG 66
           SF SFS Y  ALS T  RL+ R  S S+   E+ + R  S   M R L W+DL+    G 
Sbjct: 38  SFQSFSTYKSALSATCPRLADRLLSRSSDAYELDAARRESENPMRRCLTWWDLLWLSFGS 97

Query: 67  MIGAGVFVTTGPAAH-QAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRIT 126
           ++G+GVFV TG  A   AGPA+VLSYAI+G+ ALLS  CY EF V++PVAGG+FSYLR+ 
Sbjct: 98  VVGSGVFVITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEFGVEIPVAGGSFSYLRVE 157

Query: 127 FGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDLI 186
            G+F  F+   N++++ ++  A + RS++ YL + +   +  +R+ V    KGFD +D +
Sbjct: 158 LGDFIAFIAAGNILLEAMVGAAGLGRSWSSYLASLVKNDSDYFRIKVDSFAKGFDLLDPV 217

Query: 187 AVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNES 246
           AVAV+L+   I    TK++S +N++ + + +  I F++V GF      N           
Sbjct: 218 AVAVLLVANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFTHSKTSNLVP-------- 277

Query: 247 GFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLMA 306
            FFP+GA GV + A++V  SY G+D V+ MAEE   P++DIP+G+ GS+ ++T++YCLMA
Sbjct: 278 -FFPYGAKGVVQSAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMITVVYCLMA 337

Query: 307 ASMAKLLPYDMIKAEAPLAEAFAR--WKWVSNVIGGGASFGILTSLLVAMMGQARYMCVI 366
            ++  ++ Y  I A A  + AFA+   KW   ++G  A  G+ TSLLV  +GQARY   I
Sbjct: 338 LALTMMVKYTEIDANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLGQARYTTQI 397

Query: 367 GRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMVAN 426
            RS ++P WFA VHPKT TP+ A+  + I +++I+ F  L+VL +     TLF+F +VA 
Sbjct: 398 ARSHMIPPWFALVHPKTGTPIYATLLVTILSSIISFFTSLEVLSSVFSFATLFIFMLVAV 457

Query: 427 AIIFRRYVVIGSTKPQPTLSFLCSFSL---TAIIFTLIWHFVTPGRAKTALLTVTAIVAI 486
           A++ RRY V   T     L FL    L   ++I  + +W+    G       TVT ++  
Sbjct: 458 ALLVRRYYVKDVTPEAGLLKFLGFLFLIIASSIGVSALWNSGVKG---WIAYTVTGVIWF 517

Query: 487 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 546
           + +L    ++P+ R P+ WGVPL+PW+P  SI +NLFL+GSL   +++RF+  +++++L 
Sbjct: 518 IGTLGL-ALLPKYRVPKVWGVPLVPWLPSFSIAMNLFLIGSLGYVAFLRFIICTMVMLLY 575

Query: 547 YVLYGVHSSSD 552
           Y+  G+H++ D
Sbjct: 578 YLFVGLHATYD 575

BLAST of CmaCh04G025110.1 vs. ExPASy TrEMBL
Match: A0A6J1JUB5 (cationic amino acid transporter 6, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111487575 PE=4 SV=1)

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 574/574 (100.00%), Postives = 574/574 (100.00%), Query Frame = 0

Query: 1   MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60
           MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV
Sbjct: 27  MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 86

Query: 61  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120
           GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS
Sbjct: 87  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 146

Query: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180
           YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD
Sbjct: 147 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 206

Query: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240
           QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD
Sbjct: 207 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 266

Query: 241 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300
           PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL
Sbjct: 267 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 326

Query: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360
           YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM
Sbjct: 327 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 386

Query: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420
           CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM
Sbjct: 387 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 446

Query: 421 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480
           VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI
Sbjct: 447 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 506

Query: 481 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 540
           VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV
Sbjct: 507 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 566

Query: 541 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 575
           YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV
Sbjct: 567 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 600

BLAST of CmaCh04G025110.1 vs. ExPASy TrEMBL
Match: A0A6J1H8Q5 (cationic amino acid transporter 6, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460666 PE=4 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 565/574 (98.43%), Postives = 569/574 (99.13%), Query Frame = 0

Query: 1   MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60
           MDAHS+SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV
Sbjct: 27  MDAHSSSFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 86

Query: 61  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120
           GFGLGGM+GAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS
Sbjct: 87  GFGLGGMVGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 146

Query: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180
           YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD
Sbjct: 147 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 206

Query: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240
           QIDLIAVAVVLIITIIICYSTKQSSVVNM LTAIHILFIAFLIVFGFWKGDWKNFTEPGD
Sbjct: 207 QIDLIAVAVVLIITIIICYSTKQSSVVNMTLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 266

Query: 241 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300
           PKNESGFFPHGATGVFKGASLV LSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL
Sbjct: 267 PKNESGFFPHGATGVFKGASLVYLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 326

Query: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360
           YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM
Sbjct: 327 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 386

Query: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420
           CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM
Sbjct: 387 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 446

Query: 421 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480
           VANAII+RRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI
Sbjct: 447 VANAIIYRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 506

Query: 481 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 540
           VVSLIFNCVVPQ RKPEFWGVPLMPWIPCASIFLNLFLLGSLDG SY+RFVFFSILVVLV
Sbjct: 507 VVSLIFNCVVPQTRKPEFWGVPLMPWIPCASIFLNLFLLGSLDGMSYIRFVFFSILVVLV 566

Query: 541 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 575
           YVLYGVHSSSDAEGDDSLFVKNGEI ELQNNFKV
Sbjct: 567 YVLYGVHSSSDAEGDDSLFVKNGEILELQNNFKV 600

BLAST of CmaCh04G025110.1 vs. ExPASy TrEMBL
Match: A0A5D3CMX6 (Cationic amino acid transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold304G001030 PE=4 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 3.6e-292
Identity = 520/574 (90.59%), Postives = 546/574 (95.12%), Query Frame = 0

Query: 1   MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60
           MDAHS+SFSSFSAY  ALS TPSRLSRRAFSVSTSFDEMT+TRSTSGVHM RTLRWFDLV
Sbjct: 1   MDAHSSSFSSFSAYFHALSQTPSRLSRRAFSVSTSFDEMTTTRSTSGVHMQRTLRWFDLV 60

Query: 61  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120
           GFGLGGMIGAGVFVTTGPAA QAGPAIV+SYAIAGLCALLSAFCYTEFAVDMPVAGGAFS
Sbjct: 61  GFGLGGMIGAGVFVTTGPAALQAGPAIVISYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120

Query: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180
           YLR+TFGEFA FLTGANLIMDYVMSNAAVARSF+QYLGAAIGISTAKWRL +PGLPKGFD
Sbjct: 121 YLRVTFGEFAAFLTGANLIMDYVMSNAAVARSFSQYLGAAIGISTAKWRLHIPGLPKGFD 180

Query: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240
           QIDLIAVAVVLIIT+IICYSTKQSSVVNM LTAIHILFIAF+IVFGFWKGDWKNFTEPGD
Sbjct: 181 QIDLIAVAVVLIITVIICYSTKQSSVVNMTLTAIHILFIAFVIVFGFWKGDWKNFTEPGD 240

Query: 241 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300
           PKNESGFFPHGA GVFKGASLV LSYIGYDAVSTMAEEVR+PAKDIP+GVSGSVVLVT+L
Sbjct: 241 PKNESGFFPHGAAGVFKGASLVYLSYIGYDAVSTMAEEVRSPAKDIPIGVSGSVVLVTVL 300

Query: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360
           YCLMAASMAKLLPYDMIK EAP A AF RWKWVSNVIGGGASFGILTSLLVAMMGQARYM
Sbjct: 301 YCLMAASMAKLLPYDMIKKEAPFAAAFGRWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360

Query: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420
           CVIGRSRVVPAWFAEVHPKTSTPLNASAFLG+FTA IALF DLDVLLNFVCIGTLFVFYM
Sbjct: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGVFTAAIALFADLDVLLNFVCIGTLFVFYM 420

Query: 421 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480
           VANA+I+RRYV IGST P+PTLSFLCSFSLTAIIFTL+WHFVTPG+AKT LL+VTAIVAI
Sbjct: 421 VANAVIYRRYVEIGSTNPRPTLSFLCSFSLTAIIFTLMWHFVTPGKAKTTLLSVTAIVAI 480

Query: 481 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 540
           V+SLIF+CVVPQA KPEFWGVPLMPWIPCASIFLN+FLLGSLDGTSY+RFVFFSIL VL+
Sbjct: 481 VISLIFHCVVPQAWKPEFWGVPLMPWIPCASIFLNIFLLGSLDGTSYIRFVFFSILAVLI 540

Query: 541 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 575
           Y+LY VHSSSDAE   SL VKN E+PEL +NFKV
Sbjct: 541 YMLYSVHSSSDAEKAGSLCVKNREVPELDSNFKV 574

BLAST of CmaCh04G025110.1 vs. ExPASy TrEMBL
Match: A0A1S3C3M2 (cationic amino acid transporter 6, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103496518 PE=4 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 3.6e-292
Identity = 520/574 (90.59%), Postives = 546/574 (95.12%), Query Frame = 0

Query: 1   MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60
           MDAHS+SFSSFSAY  ALS TPSRLSRRAFSVSTSFDEMT+TRSTSGVHM RTLRWFDLV
Sbjct: 55  MDAHSSSFSSFSAYFHALSQTPSRLSRRAFSVSTSFDEMTTTRSTSGVHMQRTLRWFDLV 114

Query: 61  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120
           GFGLGGMIGAGVFVTTGPAA QAGPAIV+SYAIAGLCALLSAFCYTEFAVDMPVAGGAFS
Sbjct: 115 GFGLGGMIGAGVFVTTGPAALQAGPAIVISYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 174

Query: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180
           YLR+TFGEFA FLTGANLIMDYVMSNAAVARSF+QYLGAAIGISTAKWRL +PGLPKGFD
Sbjct: 175 YLRVTFGEFAAFLTGANLIMDYVMSNAAVARSFSQYLGAAIGISTAKWRLHIPGLPKGFD 234

Query: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240
           QIDLIAVAVVLIIT+IICYSTKQSSVVNM LTAIHILFIAF+IVFGFWKGDWKNFTEPGD
Sbjct: 235 QIDLIAVAVVLIITVIICYSTKQSSVVNMTLTAIHILFIAFVIVFGFWKGDWKNFTEPGD 294

Query: 241 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300
           PKNESGFFPHGA GVFKGASLV LSYIGYDAVSTMAEEVR+PAKDIP+GVSGSVVLVT+L
Sbjct: 295 PKNESGFFPHGAAGVFKGASLVYLSYIGYDAVSTMAEEVRSPAKDIPIGVSGSVVLVTVL 354

Query: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360
           YCLMAASMAKLLPYDMIK EAP A AF RWKWVSNVIGGGASFGILTSLLVAMMGQARYM
Sbjct: 355 YCLMAASMAKLLPYDMIKKEAPFAAAFGRWKWVSNVIGGGASFGILTSLLVAMMGQARYM 414

Query: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420
           CVIGRSRVVPAWFAEVHPKTSTPLNASAFLG+FTA IALF DLDVLLNFVCIGTLFVFYM
Sbjct: 415 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGVFTAAIALFADLDVLLNFVCIGTLFVFYM 474

Query: 421 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480
           VANA+I+RRYV IGST P+PTLSFLCSFSLTAIIFTL+WHFVTPG+AKT LL+VTAIVAI
Sbjct: 475 VANAVIYRRYVEIGSTNPRPTLSFLCSFSLTAIIFTLMWHFVTPGKAKTTLLSVTAIVAI 534

Query: 481 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 540
           V+SLIF+CVVPQA KPEFWGVPLMPWIPCASIFLN+FLLGSLDGTSY+RFVFFSIL VL+
Sbjct: 535 VISLIFHCVVPQAWKPEFWGVPLMPWIPCASIFLNIFLLGSLDGTSYIRFVFFSILAVLI 594

Query: 541 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 575
           Y+LY VHSSSDAE   SL VKN E+PEL +NFKV
Sbjct: 595 YMLYSVHSSSDAEKAGSLCVKNREVPELDSNFKV 628

BLAST of CmaCh04G025110.1 vs. ExPASy TrEMBL
Match: A0A6J1FGS4 (cationic amino acid transporter 6, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445536 PE=4 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 2.3e-291
Identity = 517/574 (90.07%), Postives = 548/574 (95.47%), Query Frame = 0

Query: 1   MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60
           MDAHS+SFSSFSAYLRALSL PSRLSRRAFSVSTSFDEMTSTR TSGVHM RTLRWFDLV
Sbjct: 1   MDAHSSSFSSFSAYLRALSLIPSRLSRRAFSVSTSFDEMTSTRYTSGVHMQRTLRWFDLV 60

Query: 61  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120
           GFGLGGMIGAGVFVTTGPA  +AGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS
Sbjct: 61  GFGLGGMIGAGVFVTTGPATQEAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120

Query: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180
           YLR+TFGEFA FLTGANLIMDYVMSNAAVARSFTQYLGAAIGIS+AKW+L++PGLPKGFD
Sbjct: 121 YLRVTFGEFAAFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISSAKWKLLIPGLPKGFD 180

Query: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240
           QIDL+AVAVVLIITIIICYSTKQSSV+NM LTAIHILFIAF+IVFGF KGDWKNF+EPGD
Sbjct: 181 QIDLVAVAVVLIITIIICYSTKQSSVLNMTLTAIHILFIAFVIVFGFCKGDWKNFSEPGD 240

Query: 241 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300
           PK+ESGFFPHGA GVFKGASLV LSYIGYDAVSTMAEEVRNPAKDIP+GVSGSVVLVT+L
Sbjct: 241 PKDESGFFPHGAAGVFKGASLVYLSYIGYDAVSTMAEEVRNPAKDIPIGVSGSVVLVTVL 300

Query: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360
           YCLMAASM KLLPYDMI+AEAP A AFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM
Sbjct: 301 YCLMAASMIKLLPYDMIQAEAPFAAAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360

Query: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420
           CVIGRSRVVPAWFAEVHPKTSTPLNASAFLG+FTA IALF DLDVLLNFVCIGTLFVFYM
Sbjct: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGVFTAAIALFADLDVLLNFVCIGTLFVFYM 420

Query: 421 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480
           VANA+I+RRYVVIGST P+PTLSFLCSFSLTAIIFTLIWHF TPG+AKTALL++TAIVAI
Sbjct: 421 VANAVIYRRYVVIGSTNPRPTLSFLCSFSLTAIIFTLIWHFATPGKAKTALLSITAIVAI 480

Query: 481 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 540
            +SLIF+CV PQA KP+ WGVPLMPWIPCASIFLN+FLLGSLDGTSY+RFVFFSIL +L+
Sbjct: 481 AISLIFHCVTPQAWKPQHWGVPLMPWIPCASIFLNIFLLGSLDGTSYIRFVFFSILTMLI 540

Query: 541 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 575
           YVLY VHSSSDAE D SL VKNGEIPEL+N+FKV
Sbjct: 541 YVLYSVHSSSDAEEDGSLRVKNGEIPELENSFKV 574

BLAST of CmaCh04G025110.1 vs. NCBI nr
Match: XP_022990793.1 (cationic amino acid transporter 6, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 574/574 (100.00%), Postives = 574/574 (100.00%), Query Frame = 0

Query: 1   MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60
           MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV
Sbjct: 27  MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 86

Query: 61  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120
           GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS
Sbjct: 87  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 146

Query: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180
           YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD
Sbjct: 147 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 206

Query: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240
           QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD
Sbjct: 207 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 266

Query: 241 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300
           PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL
Sbjct: 267 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 326

Query: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360
           YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM
Sbjct: 327 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 386

Query: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420
           CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM
Sbjct: 387 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 446

Query: 421 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480
           VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI
Sbjct: 447 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 506

Query: 481 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 540
           VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV
Sbjct: 507 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 566

Query: 541 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 575
           YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV
Sbjct: 567 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 600

BLAST of CmaCh04G025110.1 vs. NCBI nr
Match: XP_022959654.1 (cationic amino acid transporter 6, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 565/574 (98.43%), Postives = 569/574 (99.13%), Query Frame = 0

Query: 1   MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60
           MDAHS+SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV
Sbjct: 27  MDAHSSSFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 86

Query: 61  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120
           GFGLGGM+GAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS
Sbjct: 87  GFGLGGMVGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 146

Query: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180
           YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD
Sbjct: 147 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 206

Query: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240
           QIDLIAVAVVLIITIIICYSTKQSSVVNM LTAIHILFIAFLIVFGFWKGDWKNFTEPGD
Sbjct: 207 QIDLIAVAVVLIITIIICYSTKQSSVVNMTLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 266

Query: 241 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300
           PKNESGFFPHGATGVFKGASLV LSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL
Sbjct: 267 PKNESGFFPHGATGVFKGASLVYLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 326

Query: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360
           YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM
Sbjct: 327 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 386

Query: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420
           CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM
Sbjct: 387 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 446

Query: 421 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480
           VANAII+RRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI
Sbjct: 447 VANAIIYRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 506

Query: 481 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 540
           VVSLIFNCVVPQ RKPEFWGVPLMPWIPCASIFLNLFLLGSLDG SY+RFVFFSILVVLV
Sbjct: 507 VVSLIFNCVVPQTRKPEFWGVPLMPWIPCASIFLNLFLLGSLDGMSYIRFVFFSILVVLV 566

Query: 541 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 575
           YVLYGVHSSSDAEGDDSLFVKNGEI ELQNNFKV
Sbjct: 567 YVLYGVHSSSDAEGDDSLFVKNGEILELQNNFKV 600

BLAST of CmaCh04G025110.1 vs. NCBI nr
Match: XP_023523477.1 (cationic amino acid transporter 7, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 563/574 (98.08%), Postives = 570/574 (99.30%), Query Frame = 0

Query: 1   MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60
           MDAHS+SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV
Sbjct: 1   MDAHSSSFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60

Query: 61  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120
           GFGLGGM+GAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS
Sbjct: 61  GFGLGGMVGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120

Query: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180
           YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD
Sbjct: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180

Query: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240
           QIDLIAVAVVLIITIIICYSTKQSSVVNM LTAIHILFIAFLIVFGFWKGDWKNFTEPGD
Sbjct: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMTLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240

Query: 241 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300
           PKNESGFFPHGATGVFKGASLV LSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL
Sbjct: 241 PKNESGFFPHGATGVFKGASLVYLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300

Query: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360
           YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM
Sbjct: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360

Query: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420
           CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM
Sbjct: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420

Query: 421 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480
           VANAII+RRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI
Sbjct: 421 VANAIIYRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480

Query: 481 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 540
           VVSLIFNCVVPQ+RKPEFWGVPLMPWIPCASIFLNLFLLGSLDG+SY+RFVFFSILVVLV
Sbjct: 481 VVSLIFNCVVPQSRKPEFWGVPLMPWIPCASIFLNLFLLGSLDGSSYIRFVFFSILVVLV 540

Query: 541 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 575
           YVLYGVHSSSDAEGDDSLF+KN EI ELQNNFKV
Sbjct: 541 YVLYGVHSSSDAEGDDSLFLKNREILELQNNFKV 574

BLAST of CmaCh04G025110.1 vs. NCBI nr
Match: KAG7032928.1 (Cationic amino acid transporter 6, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 563/574 (98.08%), Postives = 567/574 (98.78%), Query Frame = 0

Query: 1   MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60
           MDAHS+SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV
Sbjct: 27  MDAHSSSFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 86

Query: 61  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120
           GFGLGGM+GAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS
Sbjct: 87  GFGLGGMVGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 146

Query: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180
           YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD
Sbjct: 147 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 206

Query: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240
           QIDLIAVAVVLIIT IICYSTKQSSVVNM LTAIHILFIAFLIVFGFWKGDWKNFTEPGD
Sbjct: 207 QIDLIAVAVVLIITTIICYSTKQSSVVNMTLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 266

Query: 241 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300
           PKNESGFFPHGATGVFKGASLV LSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL
Sbjct: 267 PKNESGFFPHGATGVFKGASLVYLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 326

Query: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360
           YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM
Sbjct: 327 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 386

Query: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420
           CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM
Sbjct: 387 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 446

Query: 421 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480
           VANAII+RRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI
Sbjct: 447 VANAIIYRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 506

Query: 481 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 540
           VVSLIFNCVVPQ RKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSY+RFVFFSILVVLV
Sbjct: 507 VVSLIFNCVVPQTRKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYIRFVFFSILVVLV 566

Query: 541 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 575
           YVLY VHSSSDAEGDDSLFVKNGEI ELQNN KV
Sbjct: 567 YVLYSVHSSSDAEGDDSLFVKNGEILELQNNSKV 600

BLAST of CmaCh04G025110.1 vs. NCBI nr
Match: KAG6602249.1 (Cationic amino acid transporter 7, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 562/574 (97.91%), Postives = 567/574 (98.78%), Query Frame = 0

Query: 1   MDAHSASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 60
           MDAHS+SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV
Sbjct: 27  MDAHSSSFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLV 86

Query: 61  GFGLGGMIGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 120
           GFGLGGM+GAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS
Sbjct: 87  GFGLGGMVGAGVFVTTGPAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFS 146

Query: 121 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 180
           YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD
Sbjct: 147 YLRITFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFD 206

Query: 181 QIDLIAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 240
           QIDLIAVAVVLIITIIICYSTKQSSVVNM LTAIHILFIAFLIVFGFWKGDWKNFTEPGD
Sbjct: 207 QIDLIAVAVVLIITIIICYSTKQSSVVNMTLTAIHILFIAFLIVFGFWKGDWKNFTEPGD 266

Query: 241 PKNESGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 300
           PKNESGFFPHGATGVFKGASLV LSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL
Sbjct: 267 PKNESGFFPHGATGVFKGASLVYLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTIL 326

Query: 301 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 360
           YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM
Sbjct: 327 YCLMAASMAKLLPYDMIKAEAPLAEAFARWKWVSNVIGGGASFGILTSLLVAMMGQARYM 386

Query: 361 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 420
           CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM
Sbjct: 387 CVIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYM 446

Query: 421 VANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAI 480
           VANAII+RRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPG AKTALLTVTAIVAI
Sbjct: 447 VANAIIYRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGHAKTALLTVTAIVAI 506

Query: 481 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 540
           VVSLIFNCVVPQ RKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSY+RFVFFSILV+LV
Sbjct: 507 VVSLIFNCVVPQTRKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYIRFVFFSILVMLV 566

Query: 541 YVLYGVHSSSDAEGDDSLFVKNGEIPELQNNFKV 575
           YVLY VHSSSDAEGDDSLFVKNGEI ELQNN KV
Sbjct: 567 YVLYSVHSSSDAEGDDSLFVKNGEILELQNNSKV 600

BLAST of CmaCh04G025110.1 vs. TAIR 10
Match: AT5G04770.1 (cationic amino acid transporter 6 )

HSP 1 Score: 775.4 bits (2001), Expect = 3.2e-224
Identity = 400/564 (70.92%), Postives = 465/564 (82.45%), Query Frame = 0

Query: 6   ASFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLG 65
           +SFSS   YL +LS TPSRLSRRA SVSTS DEM+  R+ SG  M RTLRW+DL+G G+G
Sbjct: 13  SSFSSLRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIG 72

Query: 66  GMIGAGVFVTTGPAAH-QAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRI 125
           GM+GAGVFVTTG A+   AGP+IV+SYAIAGLCALLSAFCYTEFAV +PVAGGAFSY+RI
Sbjct: 73  GMVGAGVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRI 132

Query: 126 TFGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDL 185
           TFGEF  F TGANL+MDYVMSNAAV+RSFT YLG A GIST+KWR VV GLPKGF++ID 
Sbjct: 133 TFGEFPAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFGISTSKWRFVVSGLPKGFNEIDP 192

Query: 186 IAVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNE 245
           +AV VVL+IT+IIC ST++SS VNM++TA HI FI F+IV GF KGD KN + P +P++ 
Sbjct: 193 VAVLVVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHP 252

Query: 246 SGFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLM 305
           SGFFP GA GVF GA++V LSYIGYDAVSTMAEEV NP KDIPVGVSGSV +VT+LYCLM
Sbjct: 253 SGFFPFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLM 312

Query: 306 AASMAKLLPYDMIKAEAPLAEAFA---RWKWVSNVIGGGASFGILTSLLVAMMGQARYMC 365
           A SM+ LLPYD+I  EAP + AF     W+WV+ V+G GASFGILTSLLVAM+GQARYMC
Sbjct: 313 AVSMSMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMC 372

Query: 366 VIGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMV 425
           VIGRSRVVP WFA++HPKTSTP+NAS FLGIFTA +ALF DL+VLLN V IGTLFVFYMV
Sbjct: 373 VIGRSRVVPFWFAKIHPKTSTPVNASTFLGIFTAALALFTDLNVLLNLVSIGTLFVFYMV 432

Query: 426 ANAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAIV 485
           ANA+IFRRYV +G TKP PTL FL  FS+T+++FTLIW  V  G+ K  +L  +A+VAI 
Sbjct: 433 ANALIFRRYVPVGPTKPWPTLCFLTLFSITSLVFTLIWKLVPEGKPKAFMLGASAVVAIA 492

Query: 486 VSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLVY 545
           + L F CVVPQARKPE WGVP MPW PC SIFLN+FLLGSLD  SYVRF FFS L+VLVY
Sbjct: 493 IVLSFQCVVPQARKPELWGVPFMPWTPCVSIFLNIFLLGSLDAPSYVRFGFFSGLIVLVY 552

Query: 546 VLYGVHSSSDAEGDDSLFVKNGEI 566
           + YGVH+SSDAE + S  VK+G++
Sbjct: 553 LFYGVHASSDAEANGSFGVKDGQV 576

BLAST of CmaCh04G025110.1 vs. TAIR 10
Match: AT3G10600.1 (cationic amino acid transporter 7 )

HSP 1 Score: 771.9 bits (1992), Expect = 3.5e-223
Identity = 399/568 (70.25%), Postives = 468/568 (82.39%), Query Frame = 0

Query: 7   SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLGG 66
           SFSS   YL +LS TPSR SRRA SVSTS+DEM+  R+ SG  M RTLRW+DL+G G+GG
Sbjct: 13  SFSSLRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 72

Query: 67  MIGAGVFVTTGPAAH-QAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRIT 126
           MIGAGVFVTTG A+   AGP+IV+SYAIAGLCALLSAFCYTEFAV +PVAGGAFSY+RIT
Sbjct: 73  MIGAGVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 132

Query: 127 FGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDLI 186
           FGEF  F+TGANLIMDYV+SNAAV+R FT YLG+A GIST++WR +V GLP GF++ID I
Sbjct: 133 FGEFPAFITGANLIMDYVLSNAAVSRGFTAYLGSAFGISTSEWRFIVSGLPNGFNEIDPI 192

Query: 187 AVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNES 246
           AV VVL +T +ICYST++SS VNM+LTA+HI FI F+IV GF KGD KN T P +P+N S
Sbjct: 193 AVIVVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPENPS 252

Query: 247 GFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLMA 306
           GFFP G +GVF GA++V LSYIGYDAVSTMAEEV++P KDIP+G+SGSV +V +LYCLMA
Sbjct: 253 GFFPFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCLMA 312

Query: 307 ASMAKLLPYDMIKAEAPLAEAFAR---WKWVSNVIGGGASFGILTSLLVAMMGQARYMCV 366
            SM+ LLPYD+I AEAP + AF++   W+WV+ V+G GASFGILTSL+VAM+GQARYMCV
Sbjct: 313 ISMSMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYMCV 372

Query: 367 IGRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMVA 426
           IGRSRVVP WFA+VHPKTSTP+NASAFLGIFTAV+ALF DL+VLLN V IGTLFVFYMVA
Sbjct: 373 IGRSRVVPIWFAKVHPKTSTPVNASAFLGIFTAVLALFTDLNVLLNLVSIGTLFVFYMVA 432

Query: 427 NAIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAIVV 486
           NA+IFRRYV +G T+P PTLSFLC FS+T+I+FTL+W     G  K  +L  + + AI +
Sbjct: 433 NAVIFRRYVTVGYTEPWPTLSFLCLFSITSILFTLVWQLAPSGPPKWFILGASTVTAIAI 492

Query: 487 SLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLVYV 546
             IF+CVVPQAR PEFWGVPLMPW PC SIFLN+FLLGSLD  SY+RF FFS LVVLVYV
Sbjct: 493 VQIFHCVVPQARIPEFWGVPLMPWTPCVSIFLNIFLLGSLDAPSYIRFGFFSGLVVLVYV 552

Query: 547 LYGVHSSSDAEGDDSLFVKNGEIPELQN 571
            Y VH+S DAEGD SL  K+ E  E  N
Sbjct: 553 FYSVHASYDAEGDGSLDFKDVESLERIN 580

BLAST of CmaCh04G025110.1 vs. TAIR 10
Match: AT4G21120.1 (amino acid transporter 1 )

HSP 1 Score: 421.8 bits (1083), Expect = 8.9e-118
Identity = 232/548 (42.34%), Postives = 343/548 (62.59%), Query Frame = 0

Query: 7   SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLGG 66
           SF S   YL+AL  TPSR   R  + S   DE+   ++ SG  M +TL W+DL+ FG+G 
Sbjct: 27  SFQSMGNYLKALKETPSRFMDRIMTRSLDSDEINEMKARSGHEMKKTLTWWDLMWFGIGA 86

Query: 67  MIGAGVFVTTG-PAAHQAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRIT 126
           +IG+G+FV TG  A + +GPA+VLSY ++G+ A+LS FCYTEFAV++PVAGG+F+YLR+ 
Sbjct: 87  VIGSGIFVLTGLEARNHSGPAVVLSYVVSGVSAMLSVFCYTEFAVEIPVAGGSFAYLRVE 146

Query: 127 FGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDLI 186
            G+F  F+   N+I++YV+  AAVARS+T Y    +      +R++V  L + +  +D I
Sbjct: 147 LGDFMAFIAAGNIILEYVVGGAAVARSWTSYFATLLNHKPEDFRIIVHKLGEDYSHLDPI 206

Query: 187 AVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNES 246
           AV V  II ++    TK SS  N + + IH++ I F+I+ GF K D KN+         S
Sbjct: 207 AVGVCAIICVLAVVGTKGSSRFNYIASIIHMVVILFVIIAGFTKADVKNY---------S 266

Query: 247 GFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLMA 306
            F P+G  GVFK A+++  +YIG+DAVSTMAEE +NP +DIP+G+ GS+V+ T+ YCLMA
Sbjct: 267 DFTPYGVRGVFKSAAVLFFAYIGFDAVSTMAEETKNPGRDIPIGLVGSMVVTTVCYCLMA 326

Query: 307 ASMAKLLPYDMIKAEAPLAEAFAR--WKWVSNVIGGGASFGILTSLLVAMMGQARYMCVI 366
            ++  + PY  I  +AP + AF+   W W   ++  GA  G+ T LLV  +GQARYM  I
Sbjct: 327 VTLCLMQPYQQIDPDAPFSVAFSAVGWDWAKYIVAFGALKGMTTVLLVGAIGQARYMTHI 386

Query: 367 GRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMVAN 426
            R+ ++P W A+V+ KT TP+NA+  +   TA+IA F  L +L + + + TLF+F  VA 
Sbjct: 387 ARAHMMPPWLAQVNAKTGTPINATVVMLAATALIAFFTKLKILADLLSVSTLFIFMFVAV 446

Query: 427 AIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTLIWHFVTPGRAKTALLTVTAIVAIVVS 486
           A++ RRY V G T  +    FL    L     T    +            +T  +  + +
Sbjct: 447 ALLVRRYYVTGETSTRDRNKFLVFLGLILASSTATAVYWALEEEGWIGYCITVPIWFLST 506

Query: 487 LIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLVYVL 546
           +    +VPQAR P+ WGVPL+PW+P ASI +N+FLLGS+D  S+VRF  ++ ++++ YVL
Sbjct: 507 VAMKFLVPQARAPKIWGVPLVPWLPSASIAINIFLLGSIDTKSFVRFAIWTGILLIYYVL 565

Query: 547 YGVHSSSD 552
           +G+H++ D
Sbjct: 567 FGLHATYD 565

BLAST of CmaCh04G025110.1 vs. TAIR 10
Match: AT2G34960.1 (cationic amino acid transporter 5 )

HSP 1 Score: 380.9 bits (977), Expect = 1.7e-105
Identity = 233/552 (42.21%), Postives = 336/552 (60.87%), Query Frame = 0

Query: 7   SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLGG 66
           SF SF +Y  ALS T SR   R  S S   +E    +  S   M R L W+DLV FG G 
Sbjct: 21  SFQSFGSYRAALSQTCSRFKNRLVSRSDDENERFELKKQSEHEMKRCLTWWDLVWFGFGS 80

Query: 67  MIGAGVFVTTGPAAH-QAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRIT 126
           +IGAG+FV TG  AH QAGPAIVLSY ++GL A+LS FCYTEFAV++PVAGG+F+YLRI 
Sbjct: 81  VIGAGIFVLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYTEFAVEIPVAGGSFAYLRIE 140

Query: 127 FGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDLI 186
            G+FA F+T  N++++ ++  AAVAR++T Y    +  S    R +   L  GF+ +D I
Sbjct: 141 LGDFAAFITAGNILLESIVGTAAVARAWTSYFATLLNRSPNALR-IKTDLSSGFNLLDPI 200

Query: 187 AVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNES 246
           AV V+     I   ST+++S++N + +AI+ L I F+I+ GF   D  N T         
Sbjct: 201 AVVVIAASATIASISTRKTSLLNWIASAINTLVIFFVIIAGFIHADTSNLTP-------- 260

Query: 247 GFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLMA 306
            F P G  GVF+ A++V  +Y G+D+++TMAEE +NP++DIP+G+ GS+ ++T++YCLMA
Sbjct: 261 -FLPFGPEGVFRAAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMSIITVIYCLMA 320

Query: 307 ASMAKLLPYDMIKAEAPLAEAF--ARWKWVSNVIGGGASFGILTSLLVAMMGQARYMCVI 366
            S++ +  Y  I   A  + AF     KW   ++  GA  G+ T LLV  +GQARY+  I
Sbjct: 321 LSLSMMQKYTDIDPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGALGQARYVTHI 380

Query: 367 GRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMVAN 426
            R+ ++P  FA VHPKT TP+NA+  + I +A+IA F  LDVL + + I TLF+F M+  
Sbjct: 381 ARTHMIPPIFALVHPKTGTPINANLLVAIPSALIAFFSGLDVLASLLSISTLFIFTMMPI 440

Query: 427 AIIFRRYVVIGSTKPQPTLSFLCSFSLTAIIFTL----IWHFVTPGRAKTALLTVTAIVA 486
           A++ RRY V   T P+  L  L +  L  ++ ++     W     G      +TV     
Sbjct: 441 ALLVRRYYVRQDT-PRVHLIKLITCLLFVVVSSMGTSAYWGMQRKGSWIGYTVTVPFWFL 500

Query: 487 IVVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVL 546
             + ++F   VPQ R P+ WGVPL+PW+PC SI  N+FL+GSL   ++VRF   ++ ++L
Sbjct: 501 GTLGIVF--FVPQQRTPKVWGVPLVPWLPCLSIATNIFLMGSLGAMAFVRFGVCTLAMLL 559

Query: 547 VYVLYGVHSSSD 552
            Y L G+H++ D
Sbjct: 561 YYFLLGLHATFD 559

BLAST of CmaCh04G025110.1 vs. TAIR 10
Match: AT1G17120.1 (cationic amino acid transporter 8 )

HSP 1 Score: 369.0 bits (946), Expect = 6.9e-102
Identity = 219/551 (39.75%), Postives = 335/551 (60.80%), Query Frame = 0

Query: 7   SFSSFSAYLRALSLTPSRLSRRAFSVSTSFDEMTSTRSTSGVHMHRTLRWFDLVGFGLGG 66
           SF SFS Y  ALS T  RL+ R  S S+   E+ + R  S   M R L W+DL+    G 
Sbjct: 38  SFQSFSTYKSALSATCPRLADRLLSRSSDAYELDAARRESENPMRRCLTWWDLLWLSFGS 97

Query: 67  MIGAGVFVTTGPAAH-QAGPAIVLSYAIAGLCALLSAFCYTEFAVDMPVAGGAFSYLRIT 126
           ++G+GVFV TG  A   AGPA+VLSYAI+G+ ALLS  CY EF V++PVAGG+FSYLR+ 
Sbjct: 98  VVGSGVFVITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEFGVEIPVAGGSFSYLRVE 157

Query: 127 FGEFAGFLTGANLIMDYVMSNAAVARSFTQYLGAAIGISTAKWRLVVPGLPKGFDQIDLI 186
            G+F  F+   N++++ ++  A + RS++ YL + +   +  +R+ V    KGFD +D +
Sbjct: 158 LGDFIAFIAAGNILLEAMVGAAGLGRSWSSYLASLVKNDSDYFRIKVDSFAKGFDLLDPV 217

Query: 187 AVAVVLIITIIICYSTKQSSVVNMMLTAIHILFIAFLIVFGFWKGDWKNFTEPGDPKNES 246
           AVAV+L+   I    TK++S +N++ + + +  I F++V GF      N           
Sbjct: 218 AVAVLLVANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFTHSKTSNLVP-------- 277

Query: 247 GFFPHGATGVFKGASLVSLSYIGYDAVSTMAEEVRNPAKDIPVGVSGSVVLVTILYCLMA 306
            FFP+GA GV + A++V  SY G+D V+ MAEE   P++DIP+G+ GS+ ++T++YCLMA
Sbjct: 278 -FFPYGAKGVVQSAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMITVVYCLMA 337

Query: 307 ASMAKLLPYDMIKAEAPLAEAFAR--WKWVSNVIGGGASFGILTSLLVAMMGQARYMCVI 366
            ++  ++ Y  I A A  + AFA+   KW   ++G  A  G+ TSLLV  +GQARY   I
Sbjct: 338 LALTMMVKYTEIDANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLGQARYTTQI 397

Query: 367 GRSRVVPAWFAEVHPKTSTPLNASAFLGIFTAVIALFVDLDVLLNFVCIGTLFVFYMVAN 426
            RS ++P WFA VHPKT TP+ A+  + I +++I+ F  L+VL +     TLF+F +VA 
Sbjct: 398 ARSHMIPPWFALVHPKTGTPIYATLLVTILSSIISFFTSLEVLSSVFSFATLFIFMLVAV 457

Query: 427 AIIFRRYVVIGSTKPQPTLSFLCSFSL---TAIIFTLIWHFVTPGRAKTALLTVTAIVAI 486
           A++ RRY V   T     L FL    L   ++I  + +W+    G       TVT ++  
Sbjct: 458 ALLVRRYYVKDVTPEAGLLKFLGFLFLIIASSIGVSALWNSGVKG---WIAYTVTGVIWF 517

Query: 487 VVSLIFNCVVPQARKPEFWGVPLMPWIPCASIFLNLFLLGSLDGTSYVRFVFFSILVVLV 546
           + +L    ++P+ R P+ WGVPL+PW+P  SI +NLFL+GSL   +++RF+  +++++L 
Sbjct: 518 IGTLGL-ALLPKYRVPKVWGVPLVPWLPSFSIAMNLFLIGSLGYVAFLRFIICTMVMLLY 575

Query: 547 YVLYGVHSSSD 552
           Y+  G+H++ D
Sbjct: 578 YLFVGLHATYD 575

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZ204.5e-22370.92Cationic amino acid transporter 6, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9SQZ05.0e-22270.25Cationic amino acid transporter 7, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q84MA51.3e-11642.34Cationic amino acid transporter 1 OS=Arabidopsis thaliana OX=3702 GN=CAT1 PE=1 S... [more]
O647592.5e-10442.21Cationic amino acid transporter 5 OS=Arabidopsis thaliana OX=3702 GN=CAT5 PE=1 S... [more]
Q9SHH09.7e-10139.75Cationic amino acid transporter 8, vacuolar OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
A0A6J1JUB50.0e+00100.00cationic amino acid transporter 6, chloroplastic-like OS=Cucurbita maxima OX=366... [more]
A0A6J1H8Q50.0e+0098.43cationic amino acid transporter 6, chloroplastic-like OS=Cucurbita moschata OX=3... [more]
A0A5D3CMX63.6e-29290.59Cationic amino acid transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C3M23.6e-29290.59cationic amino acid transporter 6, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1FGS42.3e-29190.07cationic amino acid transporter 6, chloroplastic-like OS=Cucurbita moschata OX=3... [more]
Match NameE-valueIdentityDescription
XP_022990793.10.0e+00100.00cationic amino acid transporter 6, chloroplastic-like [Cucurbita maxima][more]
XP_022959654.10.0e+0098.43cationic amino acid transporter 6, chloroplastic-like [Cucurbita moschata][more]
XP_023523477.10.0e+0098.08cationic amino acid transporter 7, chloroplastic-like [Cucurbita pepo subsp. pep... [more]
KAG7032928.10.0e+0098.08Cationic amino acid transporter 6, chloroplastic [Cucurbita argyrosperma subsp. ... [more]
KAG6602249.10.0e+0097.91Cationic amino acid transporter 7, chloroplastic, partial [Cucurbita argyrosperm... [more]
Match NameE-valueIdentityDescription
AT5G04770.13.2e-22470.92cationic amino acid transporter 6 [more]
AT3G10600.13.5e-22370.25cationic amino acid transporter 7 [more]
AT4G21120.18.9e-11842.34amino acid transporter 1 [more]
AT2G34960.11.7e-10542.21cationic amino acid transporter 5 [more]
AT1G17120.16.9e-10239.75cationic amino acid transporter 8 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029485Cationic amino acid transporter, C-terminalPFAMPF13906AA_permease_Ccoord: 499..549
e-value: 2.4E-14
score: 53.1
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 54..481
e-value: 1.8E-54
score: 185.3
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 49..489
e-value: 2.0E-96
score: 325.3
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 33..497
e-value: 6.7E-75
score: 249.9
coord: 489..569
e-value: 1.1
score: 5.0
NoneNo IPR availablePANTHERPTHR43243:SF69CATIONIC AMINO ACID TRANSPORTER 6, CHLOROPLASTICcoord: 5..565
NoneNo IPR availablePANTHERPTHR43243INNER MEMBRANE TRANSPORTER YGJI-RELATEDcoord: 5..565

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh04G025110CmaCh04G025110gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh04G025110.1:exon:2884CmaCh04G025110.1:exon:2884exon
CmaCh04G025110.1:exon:2883CmaCh04G025110.1:exon:2883exon
CmaCh04G025110.1:exon:2882CmaCh04G025110.1:exon:2882exon
CmaCh04G025110.1:exon:2881CmaCh04G025110.1:exon:2881exon
CmaCh04G025110.1:exon:2880CmaCh04G025110.1:exon:2880exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh04G025110.1:three_prime_utrCmaCh04G025110.1:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh04G025110.1:cdsCmaCh04G025110.1:cds_5CDS
CmaCh04G025110.1:cdsCmaCh04G025110.1:cds_4CDS
CmaCh04G025110.1:cdsCmaCh04G025110.1:cds_3CDS
CmaCh04G025110.1:cdsCmaCh04G025110.1:cds_2CDS
CmaCh04G025110.1:cdsCmaCh04G025110.1:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh04G025110.1:five_prime_utrCmaCh04G025110.1:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh04G025110.1CmaCh04G025110.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0003333 amino acid transmembrane transport
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0022857 transmembrane transporter activity
molecular_function GO:0015171 amino acid transmembrane transporter activity