CmaCh03G010480.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh03G010480.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	iron-sulfur assembly protein IscA, chloroplastic-like
Location	Cma_Chr03: 7240669 .. 7243250 (+)
Sequence length	635
RNA-Seq Expression	CmaCh03G010480.1
Synteny	CmaCh03G010480.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGAAAATAATATATCTTGACCGCACGATGACGATTCGCAAACATTTCCGACTAGTAAAAGGTGGCGGTTCGCATATAAACCGTTCGCCGGACTAATCGCGACCGGTTTTCTTCGATGGCGTTCTCTTCGATTACCATGAAATGCCCCCCTGTTCTTCACCTGCGAGCCCTTCCTAAAAGCTCTGCACCTCAAAGTTCAATTTCCTTTCGATATTCACCTACGAAATTCAGCCGGAGAAAAACCTTATCGATTCGATCGGTTTCAGTCCCAGGTACGCCTCTATTTTATTTTATTTTCCCTCTGCTTTCCTCCTTGATTTTTGGGTATTATATTTGACTGTATCAACGTAGTTCATCGTAGTACTGTTTGGGGGAAAATTAATCAGGAATCTGTTTCTTTTGGATTGGAACTATGAACTTGGGACGAACGTCAGGATTCGAGTTATTCTTTTAATTAATTAAACGTAGGATTCAATTTCTGGTTGCTTTGGATCGAAATTGTTAGCTCATGGAAGTTTTATATTGAAATTAAGAAACAGAATTTTGGGGATTTTCTTATTGAGTTACACGGAAAATAAATTTCCCTGCTACTTGGAACGGATGATTTGTTTGTGAGTTCTAATTCGAGATCCAGTTAGTGATAGACAAACCATGAGTGTGCATATTTGTTTTGTATCAATGCCTAAATGATTAGGATGGATTGATCTTCATGTGGGGGAAAAATGTTTATTAATTCTTGATAGCATTGATCTTTTATTCTTTTTAGAGCATACAGAATGGATTTGAAATGATTAAACAGCTCTTTTTCATGTTGAAACCACTCCGAAATATGTCTTAAATCAATTGAATGTTTGCTTTTATACTTTTAAATGCAATTTTCATACTATTTGAATGATTAAAAGTATGTTTTGGAGTGATTTTGAGTGTGAAAAAAAGTGATTTCAACCAATTTCAAAATCACTCCTAAACATGACATGCAATATGGTTTTGTGAATGGCACTTTCCCTTTGCCGCTTACTAGAGATCAAAGATAAAATCTACTGAGTCTTGCAGTTTCTGCATATGAACTATGCGTTATGATCACAGTTTTGCATTATCATCTTATGTTGTTGAACATGTAAATTGTGATTAAACAGCTGCGCCAGCATCCGAGGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTGAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACGATGGAGTTTGAGAACAGGGAAAATGCTAGACCTGATGACTCAATTATAGAATATAATGAGTTTGTGATTGGTAAGAGTTATAGCTTTTCGTTTTTCGCTCTGAATTTCTAGTGGGGTTCTTAGATTTATCCTTTTTGAATTCATTATCTTTGTAGATGACCCATTTCTTTATATATATATACTCTTAGCTCCATGCCTTCAAAAGAACATGGTGGATCTAAGTTGAGTTTTGCACGTTTTTGGATCGATACTTTCTTTCCCATGGAATGGTAGAGGGAAAGTTTTCAATTCTTCTTAGGATGAAAGAAGCTTACACTTTTATATATTCGGATATAGACGGCCATTTTGTGAGCCATATGCCATAAATGTGAGGGACTCAATGTAACCGCCCAAGCCCACCGCTAGCAAATATTGTCCGCTTTGGCTCGTCACGTATCATTGTTTGCCTCACTGTTTTAACATGCGACTATTAGGCAGATATTTCCACACTCTTATAAGTAATGTTTTGTTCTCCTCTCCAATCGTTGTGGGATCTCACCCAGCATCCTCGTTGGTACACCACTCAGTGTCTGCCTTTGATACCATTTGTAGTAACTCAAGCCCACCGCTAGCAGATATTGTTTGTTTTGGATTGAGAAATCCCACATCGGTTAGAGAGGAGAATGAAACATTTCTTATAAGAGCGTGGAAACCTCTCCCTAATAGACGTTAAAAACGTGAAACTGACGTTGATACGTAACGGGCCAAAGCGGACAATGTTTACTTGCAGTGAGCTTGGACTAATAATTTTACTCAATAGTTCATAGTCTATCTCCTCTTGGACACTCGATGACTTGAATGAATTGATATCATAATTTTTTATGCTGACTGATGAGCAGGTTCTGCAACTTTTGTCAAATATACTTTTCTTTAATCTATACTATTTCAACATGAGCTTGTATATTGTTTCGATTCCTTGACAAATGCTCTCCTACTCTATTGATAAAGGAACCTAAGCATCTTTCAGTGAACTATGAAGGTATCATTTTATCTACTTTATTTTCGACTGTGCCCTCTTAAGTCGTAGACTCGACGACGAATATGAGTTCACTTATATCACTTGCTTTATAGCTTGAGCTTTTGATACAAACTTCCCTCTCTGCTTGTTTTGTAGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGGGGCTTCTCCTTCAAGAACCCAAACGCTACACAAACTTGTGGCTGTGGAAAATCATTTGCTGCAGAAATGTAG

mRNA sequence

AAAGAAAATAATATATCTTGACCGCACGATGACGATTCGCAAACATTTCCGACTAGTAAAAGGTGGCGGTTCGCATATAAACCGTTCGCCGGACTAATCGCGACCGGTTTTCTTCGATGGCGTTCTCTTCGATTACCATGAAATGCCCCCCTGTTCTTCACCTGCGAGCCCTTCCTAAAAGCTCTGCACCTCAAAGTTCAATTTCCTTTCGATATTCACCTACGAAATTCAGCCGGAGAAAAACCTTATCGATTCGATCGGTTTCAGTCCCAGCTGCGCCAGCATCCGAGGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTGAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACGATGGAGTTTGAGAACAGGGAAAATGCTAGACCTGATGACTCAATTATAGAATATAATGAGTTTGTGATTGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGGGGCTTCTCCTTCAAGAACCCAAACGCTACACAAACTTGTGGCTGTGGAAAATCATTTGCTGCAGAAATGTAG

Coding sequence (CDS)

ATGGCGTTCTCTTCGATTACCATGAAATGCCCCCCTGTTCTTCACCTGCGAGCCCTTCCTAAAAGCTCTGCACCTCAAAGTTCAATTTCCTTTCGATATTCACCTACGAAATTCAGCCGGAGAAAAACCTTATCGATTCGATCGGTTTCAGTCCCAGCTGCGCCAGCATCCGAGGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTGAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACGATGGAGTTTGAGAACAGGGAAAATGCTAGACCTGATGACTCAATTATAGAATATAATGAGTTTGTGATTGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGGGGCTTCTCCTTCAAGAACCCAAACGCTACACAAACTTGTGGCTGTGGAAAATCATTTGCTGCAGAAATGTAG

Protein sequence

MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEYNEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM

Homology

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BLAST of CmaCh03G010480.1 vs. ExPASy Swiss-Prot
Match: Q9XIK3 (Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISCA PE=2 SV=2)

HSP 1 Score: 238.0 bits (606), Expect = 7.7e-62
Identity = 118/149 (79.19%), Postives = 128/149 (85.91%), Query Frame = 0


Query: 27  SSISF---RYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHLNKMRSERNE 86
           +SISF    Y     +RR  LS+RS SVPAAPA EG+ PAISL++NALKHL+KMRSER E
Sbjct: 32  NSISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGE 91

Query: 87  DLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEYNEFVIVCDPKSLLFLFGMQLDYS 146
           DLCLRIGV+QGGCSGMSYTM+FENR NARPDDS IEY  F IVCDPKS+LFLFGMQLDYS
Sbjct: 92  DLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYS 151

Query: 147 DALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           DALIGGGFSF NPNATQTCGCGKSFAAEM
Sbjct: 152 DALIGGGFSFSNPNATQTCGCGKSFAAEM 180

[Close]

BLAST of CmaCh03G010480.1 vs. ExPASy Swiss-Prot
Match: P72731 (Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1417 PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 2.8e-35
Identity = 67/108 (62.04%), Postives = 82/108 (75.93%), Query Frame = 0


Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120
           A  I L+D ALKHL  ++ ++ +DLCLR+GVRQGGCSGMSY M+FE    A   D + +Y
Sbjct: 9   AKGIQLSDAALKHLLALKEQQGKDLCLRVGVRQGGCSGMSYMMDFEEPNRATEHDEVFDY 68

Query: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 169
             F I+CD KSLL+L+G+ LDYS+ALIGGGF F NPNA QTCGCGKSF
Sbjct: 69  EGFQIICDRKSLLYLYGLMLDYSNALIGGGFQFTNPNANQTCGCGKSF 116

[Close]

BLAST of CmaCh03G010480.1 vs. ExPASy Swiss-Prot
Match: P51217 (Uncharacterized protein ycf83 OS=Porphyra purpurea OX=2787 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 8.3e-32
Identity = 59/106 (55.66%), Postives = 82/106 (77.36%), Query Frame = 0


Query: 64  ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEYNEF 123
           I+LT+ A + +  ++S  + ++CLRIGV+QGGCSGMSY+M FE+  + R  D ++  + F
Sbjct: 8   ITLTEPAARQIKILKSNFDNEVCLRIGVKQGGCSGMSYSMNFESVSSLRETDEVLMLDSF 67

Query: 124 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           ++ CDPKSLL+L+G+ LDYS  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 68  LVACDPKSLLYLYGLSLDYSSELIGGGFQFSNPNASQTCGCGKSFS 113

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BLAST of CmaCh03G010480.1 vs. ExPASy Swiss-Prot
Match: Q1XDR9 (Uncharacterized protein ycf83 OS=Pyropia yezoensis OX=2788 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 9.2e-31
Identity = 61/112 (54.46%), Postives = 82/112 (73.21%), Query Frame = 0


Query: 58  EGIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSI 117
           EG    I++T  ALK +  ++++   D+ LRIGVRQGGCSGMSY+M FE+ +  +  D  
Sbjct: 2   EGNMGFITITKPALKQIAILKNDHENDVHLRIGVRQGGCSGMSYSMNFEHVDKLKDTDER 61

Query: 118 IEYNEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           +  + F +VCDPKSLL+L+G+ LD+S  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 62  LRLDNFSVVCDPKSLLYLYGLSLDFSSELIGGGFQFSNPNASQTCGCGKSFS 113

[Close]

BLAST of CmaCh03G010480.1 vs. ExPASy Swiss-Prot
Match: Q9MSA1 (Uncharacterized protein ycf83 OS=Galdieria sulphuraria OX=130081 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.7e-29
Identity = 58/107 (54.21%), Postives = 80/107 (74.77%), Query Frame = 0


Query: 64  ISLTDNALKHLNKMRSERNED-LCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEYNE 123
           I++T +AL HLN +    +ED L +RIG++QGGCSG+SY M +E + N   +D + +Y+ 
Sbjct: 6   INITKSALDHLNSISKLNSEDKLYIRIGIKQGGCSGLSYFMTYEKKSNISDNDLVYDYDN 65

Query: 124 FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           F +VCD KS+L+L+G+ LDYS +LI GGF F NPNA QTCGCGKSF+
Sbjct: 66  FTLVCDNKSILYLYGISLDYSSSLIDGGFKFLNPNAKQTCGCGKSFS 112

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BLAST of CmaCh03G010480.1 vs. ExPASy TrEMBL
Match: A0A6J1ISS5 (iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478089 PE=3 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.2e-91
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120

Query: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of CmaCh03G010480.1 vs. ExPASy TrEMBL
Match: A0A6J1GEF0 (iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111453419 PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 7.0e-90
Identity = 170/172 (98.84%), Postives = 170/172 (98.84%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of CmaCh03G010480.1 vs. ExPASy TrEMBL
Match: A0A1S3B105 (iron-sulfur assembly protein IscA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484688 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 5.2e-85
Identity = 163/172 (94.77%), Postives = 166/172 (96.51%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMK PPVLHLRALPKSSAPQSSISFR+SPTKFSR K+LSIRSVSVPAAPASE I
Sbjct: 1   MAFSSITMKFPPVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASESI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120
           APAISLTDNALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

[Close]

BLAST of CmaCh03G010480.1 vs. ExPASy TrEMBL
Match: A0A6J1BTR2 (iron-sulfur assembly protein IscA, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005271 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 3.1e-82
Identity = 158/172 (91.86%), Postives = 163/172 (94.77%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSS TMK P VLHLRALP+SSA QSSISFRYS TKFSRRK+LS+RSVSVPAAP SEGI
Sbjct: 1   MAFSSATMKFPSVLHLRALPESSATQSSISFRYSLTKFSRRKSLSVRSVSVPAAPTSEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120
           APAISLTDNAL HLNKMRS+RNEDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALNHLNKMRSDRNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAA+M
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAADM 172

[Close]

BLAST of CmaCh03G010480.1 vs. ExPASy TrEMBL
Match: A0A0A0KJ66 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486950 PE=3 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.2e-81
Identity = 159/174 (91.38%), Postives = 164/174 (94.25%), Query Frame = 0


Query: 1   MAFSSITMK--CPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASE 60
           MAFSSI+MK  CPPVLHL ALPKS APQ+SISFR+  TKFSRRK+LSIRSVSVPAAPASE
Sbjct: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSII 120
           GIAPAISLTD ALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFE+R NARPDDSII
Sbjct: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120

Query: 121 EYNEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           EYN FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 174

[Close]

BLAST of CmaCh03G010480.1 vs. NCBI nr
Match: XP_022977954.1 (iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 344.7 bits (883), Expect = 4.5e-91
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120

Query: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of CmaCh03G010480.1 vs. NCBI nr
Match: XP_022950286.1 (iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata] >XP_023544833.1 iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6604246.1 Iron-sulfur assembly protein IscA, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 339.7 bits (870), Expect = 1.4e-89
Identity = 170/172 (98.84%), Postives = 170/172 (98.84%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of CmaCh03G010480.1 vs. NCBI nr
Match: KAG7034403.1 (Iron-sulfur assembly protein IscA, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 338.2 bits (866), Expect = 4.2e-89
Identity = 169/172 (98.26%), Postives = 170/172 (98.84%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSIT+KCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITVKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of CmaCh03G010480.1 vs. NCBI nr
Match: XP_038880838.1 (iron-sulfur assembly protein IscA, chloroplastic [Benincasa hispida])

HSP 1 Score: 330.5 bits (846), Expect = 8.7e-87
Identity = 164/172 (95.35%), Postives = 169/172 (98.26%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMKC P+LHLRALP+SSAPQSSISFRYSPTKFSRRK+LSIRSVSVPAAPA+EGI
Sbjct: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAE+
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 172

[Close]

BLAST of CmaCh03G010480.1 vs. NCBI nr
Match: XP_008440129.1 (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo])

HSP 1 Score: 323.6 bits (828), Expect = 1.1e-84
Identity = 163/172 (94.77%), Postives = 166/172 (96.51%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMK PPVLHLRALPKSSAPQSSISFR+SPTKFSR K+LSIRSVSVPAAPASE I
Sbjct: 1   MAFSSITMKFPPVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASESI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120
           APAISLTDNALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

[Close]

BLAST of CmaCh03G010480.1 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 238.0 bits (606), Expect = 5.5e-63
Identity = 118/149 (79.19%), Postives = 128/149 (85.91%), Query Frame = 0


Query: 27  SSISF---RYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHLNKMRSERNE 86
           +SISF    Y     +RR  LS+RS SVPAAPA EG+ PAISL++NALKHL+KMRSER E
Sbjct: 32  NSISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGE 91

Query: 87  DLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEYNEFVIVCDPKSLLFLFGMQLDYS 146
           DLCLRIGV+QGGCSGMSYTM+FENR NARPDDS IEY  F IVCDPKS+LFLFGMQLDYS
Sbjct: 92  DLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYS 151

Query: 147 DALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           DALIGGGFSF NPNATQTCGCGKSFAAEM
Sbjct: 152 DALIGGGFSFSNPNATQTCGCGKSFAAEM 180

[Close]

BLAST of CmaCh03G010480.1 vs. TAIR 10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.1e-14
Identity = 52/159 (32.70%), Postives = 74/159 (46.54%), Query Frame = 0


Query: 17  RALPKSSAP--QSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHL 76
           R+L K  AP     I   +    FS    +   S S  + P S      + L+DN ++ +
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSS-NDVVHLSDNCIRRM 62

Query: 77  NKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEYNEFVIVCDPKSLLF 136
            +++S   E   LR+GV  GGCSG  Y  E +NR N  PDD + E N   +V D  S   
Sbjct: 63  KELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTN--PDDRVFEKNGVKLVVDNVSYDL 122

Query: 137 LFGMQLDYSDALIGGGFSFK-NPNATQTCGCGKSFAAEM 173
           + G  +DY + LI   F    NP+A   C C  SF  ++
Sbjct: 123 VKGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVKL 158

[Close]

BLAST of CmaCh03G010480.1 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.3e-11
Identity = 33/105 (31.43%), Postives = 60/105 (57.14%), Query Frame = 0


Query: 64  ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEYNEF 123
           ++L+D A   + ++   + +   LR+ V   GC+G+SY + +   +     D ++E    
Sbjct: 6   LALSDTAAARIRQLLQHQQKPF-LRLAVEAKGCNGLSYVLNYAQEKG--KFDEVVEEKGV 65

Query: 124 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 169
            I+ DPK+++ + G ++D+ D  +   F F NPNAT+ CGCG+SF
Sbjct: 66  KILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNATK-CGCGESF 106

The following BLAST results are available for this feature:

BLAST of CmaCh03G010480.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XIK3	7.7e-62	79.19	Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
P72731	2.8e-35	62.04	Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ...	[more]
P51217	8.3e-32	55.66	Uncharacterized protein ycf83 OS=Porphyra purpurea OX=2787 GN=ycf83 PE=3 SV=1	[more]
Q1XDR9	9.2e-31	54.46	Uncharacterized protein ycf83 OS=Pyropia yezoensis OX=2788 GN=ycf83 PE=3 SV=1	[more]
Q9MSA1	1.7e-29	54.21	Uncharacterized protein ycf83 OS=Galdieria sulphuraria OX=130081 GN=ycf83 PE=3 S...	[more]

BLAST of CmaCh03G010480.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ISS5	2.2e-91	100.00	iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita maxima OX=366...	[more]
A0A6J1GEF0	7.0e-90	98.84	iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita moschata OX=3...	[more]
A0A1S3B105	5.2e-85	94.77	iron-sulfur assembly protein IscA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1BTR2	3.1e-82	91.86	iron-sulfur assembly protein IscA, chloroplastic isoform X1 OS=Momordica charant...	[more]
A0A0A0KJ66	1.2e-81	91.38	Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486950...	[more]

BLAST of CmaCh03G010480.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022977954.1	4.5e-91	100.00	iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita maxima]	[more]
XP_022950286.1	1.4e-89	98.84	iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata] >XP_0...	[more]
KAG7034403.1	4.2e-89	98.26	Iron-sulfur assembly protein IscA, chloroplastic [Cucurbita argyrosperma subsp. ...	[more]
XP_038880838.1	8.7e-87	95.35	iron-sulfur assembly protein IscA, chloroplastic [Benincasa hispida]	[more]
XP_008440129.1	1.1e-84	94.77	PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo]	[more]

BLAST of CmaCh03G010480.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G10500.1	5.5e-63	79.19	chloroplast-localized ISCA-like protein	[more]
AT5G03905.1	2.1e-14	32.70	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	1.3e-11	31.43	Iron-sulphur cluster biosynthesis family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 64..169 e-value: 3.9E-37 score: 124.7
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 64..166 e-value: 1.2E-21 score: 77.0
IPR035903	HesB-like domain superfamily	GENE3D	2.60.300.12		coord: 63..171 e-value: 3.5E-35 score: 122.4
IPR035903	HesB-like domain superfamily	SUPERFAMILY	89360	HesB-like domain	coord: 63..159
None	No IPR available	PANTHER	PTHR47265:SF1	IRON-SULFUR ASSEMBLY PROTEIN ISCA, CHLOROPLASTIC	coord: 1..172
IPR031108	FeS assembly protein IscA, plant/cyanobacteria type	PANTHER	PTHR47265	IRON-SULFUR ASSEMBLY PROTEIN ISCA, CHLOROPLASTIC	coord: 1..172
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 151..168

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh03G010480	CmaCh03G010480	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh03G010480.1:exon:654	CmaCh03G010480.1:exon:654	exon
CmaCh03G010480.1:exon:655	CmaCh03G010480.1:exon:655	exon
CmaCh03G010480.1:exon:656	CmaCh03G010480.1:exon:656	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh03G010480.1:five_prime_utr	CmaCh03G010480.1:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh03G010480.1:cds	CmaCh03G010480.1:cds	CDS
CmaCh03G010480.1:cds	CmaCh03G010480.1:cds_2	CDS
CmaCh03G010480.1:cds	CmaCh03G010480.1:cds_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh03G010480.1	CmaCh03G010480.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0030674	protein-macromolecule adaptor activity