CmUC09G165140.1 (mRNA) Watermelon (USVL531) v1

Overview
NameCmUC09G165140.1
TypemRNA
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionPectinesterase
LocationCmU531Chr09: 4547910 .. 4551766 (-)
Sequence length1835
RNA-Seq ExpressionCmUC09G165140.1
SyntenyCmUC09G165140.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCATTCCCCATCTCAAAAGCCTCAAATTAAACTTCCCTCCTCTCTCCAATGGCTGCCAGAACAACCACCGCCGCCGCCTTCTACTTTCTCATCCTCATTTCCCTTTACATTTCCACTTCCGCCGCCCCCACCGGTTCAGTATGCAGCTCGACTCGAGACCCATCCTACTGCAAATCGGTTCTCCCGAATCAAACCGGCAATGTCTACTCGTACGGACGGTCGTCGTTCAGAAAGTCACTGTCGTCGTCGCAGAAATTCCTCCGGCTGGTGGAGAAATACCTCAGGTCCCGATCGTCGCTGACAGTGCCGGCGGTGAGGGCGCTGGAGGACTGCCAGCTGCTGGCGGGACTGAACATTGATTACTTAACGACGTCGTTCCGGGCGGTGAACTCGACGAGCAGAACGTTGCCGGAGATGAAGGCGGACGACGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGATGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTCAAAAATGGCCTTTCTCTCCCACTTGCTGCTGACACCAAGCTCTACTCTCTGTCCTTGGCCTTCTTTACCAAAGGTTTCTCATTATTTTTCTTTTATTTTTAAAGGATAAGAAGAAAATTCTTTAACCCACCAAAAACAAAAAAAACAAAGGATAGAGAGAAAAAAAAACTAGCTTATTTCTATTATATATTTTATTAATAATTTGATTTTAGAAAATAATTATACCTCTATACCTTAAATTAGTTTCTAAGAGTCTTTCATTATTATTATTATAGAAATTGATTAAATTAGTATAGTAGTTTTCAAAAAAAAGTAATATATATCTCCAATTTATAAAGGTTTATTTAAACTATCTAAACTTAAATTAGATCTGAAAATATATGAAGCAAAATAAATATTAAAGTGAGAGAGCAACTCTAGAGGCATTTGTCTAATTTATCTTTTAATTTTTTTTCTTTTTTCTATAGACCATCAATTAATAGAGACTCTTAATTATTTATTTTAATTATTTTGGGATTTAATGAATTAGATAGGGATTTTAACTTTTGACCTTTTAGTCAAAATACTTGCTAGATACCTTATTGATACTTTATTAAATGTAATAGTTCATTAATTTTTTAAAAGAAGAAAAATCTTAGGCAATAGTTTATAAGAGATTTGATTGATTTTTTTTTTTTTTTTCGTTTATAAACTAATATGATATATATATATATATATATTAATTAATTAATTAATGCTACGATGAAAAGTTTTGTAATAACTTAAACATCCCTAAGAAATTTTGTAGAATTGTCCTTTTTTTTTTTTAAAACATAGAAATGCCCTTCTTTTCTTTTTCCACTTTTCTTTTGTTGAGTAACAAACTTCTGTTGTAGTTAGGTGAATTTGTTGAAAGTTTTATTAGGCTTCTTGTAATTTTTTAGGTATTAATTGTTGAAATTTGTCACCCATCCATGTGTCTCTTTTCTCAAGGCTTAACTTAAAAAAAAGTTAGAAGTTGAAAATATCTTCCTTTTTCTAATATATATATATATATATTGCATGAATTAAAATATTAAACACCTAGCAAATATAAAATATTTTCATTTTCAGTTAGAAAATAAGTAAACAAAAACTAATTCGTTTTTTTTTTTTTTTTAATTTATTATTTACCAATTAATTGACTTAACATTATTGATTTTCTTTTTTTTTTCTTAATATCTTCGACAGGTTGGGTTCCAAAGAAGAAGAAAAAACCGACATGGAAAGCCGCCGGAAGACAAGGGAGGTTCAGAAATGGGCGAATGTCACTCAAAATGTCCAGCCGAACCAAAGCGATCTACGAGACGGCGACCCGCCGGAATCTGTTGCAGATCGATGACGGCGGCGACGACCAGATTGCGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACCACCATCAATGAGGCGATTGCGGCGGCGCCGAACAACTCTGCTCCGTCCGACGGGTATTTCTTAATCTTCGTGTCGGCCGGAATTTATGAAGAATATGTGTCGATTGCGAAGAACAAGAGGTACTTGATGATGATCGGAGATGGCATTAATCAGACCGTCGTCACCGGTAATCGGAGTGTCGTTGACGGCTGGACCACCTTCAATTCCGCCACGTTTGGTAAGTGTTTCTACCTTTAAATTTTATTAAATAACAATGTTAGGTTAGACCCGAATTTTTAAAAAAGTTTTCCATAGATTCCCTGGTTTTTTATTTTTATTTTTTGGTATTTTTTGAACCTATAAGTTTGATTTTTTTTTTCTTTTTTTGTCTTTTACCTATTTCATAATTTTCTAAATCATCAAAATTTAGTTTAATATGTTATTAAATAAAATATTCAATTTTATGTTAAATAAATTTATTAATTTTAAATAATATTAAATTTATTAATTATTTATTATCATAAAATTAAAAAATTCTAGAAATCATTAAATATAAAATTTAAAAATCCACGAACATATTAAATAGAAATACCAAAGTTAATAGGCCAAATCTATAATTTATCTTACACTCAGAAATTGACTAAAGAAATGCTTAGATTTTTAATCATTACTTGATTTATAGTTATTATCGGTTTTTGAAACTTAGATTTTTAAACACTACTCCCATCAATTACATATAAATTTATTATTATTATTTTTTATTAATCTTCTACTAAAGTTAAAAAAAAAAAAAAAAAAAAAGTTGGCTTCATTGGAAAACAACTTGTTTTTTGATAATAAATTTGCTCTTTTTGAAAATTAAGTTGAAAACAATAATTTCCATCCTTATGTTTTAAAAAGACTGTAAATTCTGAAAACTAAAAACAGTTCAAAAAACTTTTTTCCTTTTTTGCACTTGACTAGAAATTATGTATTAAGTATTTTTTTTTAATTAACAAAGGTAAAAATTAAAAATAGAGGAAACAAACATAGAATTCAAAAAAGAAAATTAAAAACGAAATCGATATTATTAAAAAATAAAAACAAAAAACCTAATTTATCCCCTGTTTTCGTTTCATCACCAGCGGTGGTCGGACCTGGATTCGTAGCAGTAAACATGACATTCAGAAACACCGCCGGCGCCATAAAGCATCAAGCCGTAGCCGTCCGGAATGGCGCCGACTTATCAACATTCTACCTCTGCAGCTTCGAAGCCTACCAAGACACCTTATACACCCATTCCCTCCGCCAATTCTACCGCGACTGCGACATCTACGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTCCAGAACTGCAACATTTACCCACGCCTCCCGATGTCCAACCAGTTCAACGCCATCACCGCCCAGGGTCGGACCGACCCAAATCAGAACACCGGCACCTCCATCTACAACTGCCGAATCACGGCAGCCGACGATCTGGCCAACAACAGCGGCGCCGGCGTCAAGACGTTCCTAGGGCGGCCGTGGAAGGAATACTCAAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTGATCCGGAGGGATGGAGGGCTTGGAATGGGGATTTTGCTTTGAACACTTCGTATTATGCTGAATTTGGGAATTTTGGGCCGGGATCTAACACATCGGAGAGGGTTAATTGGCCGGGATTTCATTTGATTAATGATACCGATGCTGGCAATTTCACGGCGGGGAATTTTGTGTTGGCCGACGATTGGCTGCCGCAGACCGGCGTTCCCTATACCAGCGGCCTCACGGACTAGAAAACAGGGCAAATTTGTAATTTCATGTTTACTTTTTATTTTTATTTTTTTAAAATTCCAAGGCCAAAGTGTATAGCTATGGAATGAATATGATTGATTCTTTTGTACGGTG

mRNA sequence

CCCATTCCCCATCTCAAAAGCCTCAAATTAAACTTCCCTCCTCTCTCCAATGGCTGCCAGAACAACCACCGCCGCCGCCTTCTACTTTCTCATCCTCATTTCCCTTTACATTTCCACTTCCGCCGCCCCCACCGGTTCAGTATGCAGCTCGACTCGAGACCCATCCTACTGCAAATCGGTTCTCCCGAATCAAACCGGCAATGTCTACTCGTACGGACGGTCGTCGTTCAGAAAGTCACTGTCGTCGTCGCAGAAATTCCTCCGGCTGGTGGAGAAATACCTCAGGTCCCGATCGTCGCTGACAGTGCCGGCGGTGAGGGCGCTGGAGGACTGCCAGCTGCTGGCGGGACTGAACATTGATTACTTAACGACGTCGTTCCGGGCGGTGAACTCGACGAGCAGAACGTTGCCGGAGATGAAGGCGGACGACGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGATGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTCAAAAATGGCCTTTCTCTCCCACTTGCTGCTGACACCAAGCTCTACTCTCTGTCCTTGGCCTTCTTTACCAAAGGTTGGGTTCCAAAGAAGAAGAAAAAACCGACATGGAAAGCCGCCGGAAGACAAGGGAGGTTCAGAAATGGGCGAATGTCACTCAAAATGTCCAGCCGAACCAAAGCGATCTACGAGACGGCGACCCGCCGGAATCTGTTGCAGATCGATGACGGCGGCGACGACCAGATTGCGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACCACCATCAATGAGGCGATTGCGGCGGCGCCGAACAACTCTGCTCCGTCCGACGGGTATTTCTTAATCTTCGTGTCGGCCGGAATTTATGAAGAATATGTGTCGATTGCGAAGAACAAGAGGTACTTGATGATGATCGGAGATGGCATTAATCAGACCGTCGTCACCGGTAATCGGAGTGTCGTTGACGGCTGGACCACCTTCAATTCCGCCACGTTTGCGGTGGTCGGACCTGGATTCGTAGCAGTAAACATGACATTCAGAAACACCGCCGGCGCCATAAAGCATCAAGCCGTAGCCGTCCGGAATGGCGCCGACTTATCAACATTCTACCTCTGCAGCTTCGAAGCCTACCAAGACACCTTATACACCCATTCCCTCCGCCAATTCTACCGCGACTGCGACATCTACGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTCCAGAACTGCAACATTTACCCACGCCTCCCGATGTCCAACCAGTTCAACGCCATCACCGCCCAGGGTCGGACCGACCCAAATCAGAACACCGGCACCTCCATCTACAACTGCCGAATCACGGCAGCCGACGATCTGGCCAACAACAGCGGCGCCGGCGTCAAGACGTTCCTAGGGCGGCCGTGGAAGGAATACTCAAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTGATCCGGAGGGATGGAGGGCTTGGAATGGGGATTTTGCTTTGAACACTTCGTATTATGCTGAATTTGGGAATTTTGGGCCGGGATCTAACACATCGGAGAGGGTTAATTGGCCGGGATTTCATTTGATTAATGATACCGATGCTGGCAATTTCACGGCGGGGAATTTTGTGTTGGCCGACGATTGGCTGCCGCAGACCGGCGTTCCCTATACCAGCGGCCTCACGGACTAGAAAACAGGGCAAATTTGTAATTTCATGTTTACTTTTTATTTTTATTTTTTTAAAATTCCAAGGCCAAAGTGTATAGCTATGGAATGAATATGATTGATTCTTTTGTACGGTG

Coding sequence (CDS)

ATGGCTGCCAGAACAACCACCGCCGCCGCCTTCTACTTTCTCATCCTCATTTCCCTTTACATTTCCACTTCCGCCGCCCCCACCGGTTCAGTATGCAGCTCGACTCGAGACCCATCCTACTGCAAATCGGTTCTCCCGAATCAAACCGGCAATGTCTACTCGTACGGACGGTCGTCGTTCAGAAAGTCACTGTCGTCGTCGCAGAAATTCCTCCGGCTGGTGGAGAAATACCTCAGGTCCCGATCGTCGCTGACAGTGCCGGCGGTGAGGGCGCTGGAGGACTGCCAGCTGCTGGCGGGACTGAACATTGATTACTTAACGACGTCGTTCCGGGCGGTGAACTCGACGAGCAGAACGTTGCCGGAGATGAAGGCGGACGACGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGATGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTCAAAAATGGCCTTTCTCTCCCACTTGCTGCTGACACCAAGCTCTACTCTCTGTCCTTGGCCTTCTTTACCAAAGGTTGGGTTCCAAAGAAGAAGAAAAAACCGACATGGAAAGCCGCCGGAAGACAAGGGAGGTTCAGAAATGGGCGAATGTCACTCAAAATGTCCAGCCGAACCAAAGCGATCTACGAGACGGCGACCCGCCGGAATCTGTTGCAGATCGATGACGGCGGCGACGACCAGATTGCGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACCACCATCAATGAGGCGATTGCGGCGGCGCCGAACAACTCTGCTCCGTCCGACGGGTATTTCTTAATCTTCGTGTCGGCCGGAATTTATGAAGAATATGTGTCGATTGCGAAGAACAAGAGGTACTTGATGATGATCGGAGATGGCATTAATCAGACCGTCGTCACCGGTAATCGGAGTGTCGTTGACGGCTGGACCACCTTCAATTCCGCCACGTTTGCGGTGGTCGGACCTGGATTCGTAGCAGTAAACATGACATTCAGAAACACCGCCGGCGCCATAAAGCATCAAGCCGTAGCCGTCCGGAATGGCGCCGACTTATCAACATTCTACCTCTGCAGCTTCGAAGCCTACCAAGACACCTTATACACCCATTCCCTCCGCCAATTCTACCGCGACTGCGACATCTACGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTCCAGAACTGCAACATTTACCCACGCCTCCCGATGTCCAACCAGTTCAACGCCATCACCGCCCAGGGTCGGACCGACCCAAATCAGAACACCGGCACCTCCATCTACAACTGCCGAATCACGGCAGCCGACGATCTGGCCAACAACAGCGGCGCCGGCGTCAAGACGTTCCTAGGGCGGCCGTGGAAGGAATACTCAAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTGATCCGGAGGGATGGAGGGCTTGGAATGGGGATTTTGCTTTGAACACTTCGTATTATGCTGAATTTGGGAATTTTGGGCCGGGATCTAACACATCGGAGAGGGTTAATTGGCCGGGATTTCATTTGATTAATGATACCGATGCTGGCAATTTCACGGCGGGGAATTTTGTGTTGGCCGACGATTGGCTGCCGCAGACCGGCGTTCCCTATACCAGCGGCCTCACGGACTAG

Protein sequence

MAARTTTAAAFYFLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDDGGDDQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGLTD
Homology
BLAST of CmUC09G165140.1 vs. NCBI nr
Match: XP_038896831.1 (probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida])

HSP 1 Score: 1074.3 bits (2777), Expect = 4.3e-310
Identity = 540/561 (96.26%), Postives = 549/561 (97.86%), Query Frame = 0

Query: 1   MAART----TTAAAFYFLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYG 60
           MAA T    T AAAFYFLILISLYI+TSAAPTGS CSSTRDPSYCKSVLPNQTGNVYSYG
Sbjct: 1   MAAGTIPAATAAAAFYFLILISLYITTSAAPTGSECSSTRDPSYCKSVLPNQTGNVYSYG 60

Query: 61  RSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST 120
           RSSFRKSLS+SQKFLRLVEK+LRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST
Sbjct: 61  RSSFRKSLSASQKFLRLVEKHLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST 120

Query: 121 SRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAF 180
           SRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAF
Sbjct: 121 SRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAF 180

Query: 181 FTKGWVPKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDDGGDDQI 240
           FTKGWVPKKKKKPTWKAAGR G FRNGRMSLKMSSR KAIYETATRRNLLQ DDGGDDQI
Sbjct: 181 FTKGWVPKKKKKPTWKAAGRHGGFRNGRMSLKMSSRAKAIYETATRRNLLQADDGGDDQI 240

Query: 241 AVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLM 300
           AVRDIVVVSQDGSGNFTTINEA+AAAPNNSAP+DGYFLIFVSAG+YEEYVSIAKNKRYLM
Sbjct: 241 AVRDIVVVSQDGSGNFTTINEAVAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLM 300

Query: 301 MIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG 360
           MIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG
Sbjct: 301 MIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG 360

Query: 361 ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN 420
           ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN
Sbjct: 361 ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN 420

Query: 421 QFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSF 480
           QFNAITAQGRTDPNQNTGTSIYNCRITAADDLANN+GAGVKTFLGRPWKEYSRTVYMQSF
Sbjct: 421 QFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNTGAGVKTFLGRPWKEYSRTVYMQSF 480

Query: 481 MDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN 540
           MDDLI+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN
Sbjct: 481 MDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN 540

Query: 541 FVLADDWLPQTGVPYTSGLTD 558
           FVLADDWLPQTGVPYTSGLTD
Sbjct: 541 FVLADDWLPQTGVPYTSGLTD 561

BLAST of CmUC09G165140.1 vs. NCBI nr
Match: XP_008451544.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Cucumis melo] >KAA0046659.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa] >TYK18194.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa])

HSP 1 Score: 1047.3 bits (2707), Expect = 4.5e-302
Identity = 526/557 (94.43%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 3   ARTTTAAAFYFLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRK 62
           A  TTAAAFY LILISLYI+TSA+PTGSVCSST DPSYCKS LPNQTGNVYSYGRSSFRK
Sbjct: 2   AAGTTAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRK 61

Query: 63  SLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPE 122
           SLSSSQKFLRLVEK+LRSRSSLTV AVRALEDCQLLAGLNIDYL TSF+AVN+TSR L E
Sbjct: 62  SLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRALTE 121

Query: 123 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWV 182
           MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFFTKGWV
Sbjct: 122 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWV 181

Query: 183 PKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDD--GGDDQIAVRD 242
           PKKKKKPTWKAAGRQG FRNGRMSLKMSSRT+AIYETATRRNLLQ DD  G DDQIAVRD
Sbjct: 182 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIAVRD 241

Query: 243 IVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGD 302
           IVVVSQDG+GNFTTINEAIAAAPNNSAP+DGYFLIFVSAG+YEEYVSIAKNKRYLMMIGD
Sbjct: 242 IVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 301

Query: 303 GINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLS 362
           GINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RNGADLS
Sbjct: 302 GINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGADLS 361

Query: 363 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 422
           TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA
Sbjct: 362 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 421

Query: 423 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 482
           ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL
Sbjct: 422 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 481

Query: 483 IDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 542
           I+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA
Sbjct: 482 INPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 541

Query: 543 DDWLPQTGVPYTSGLTD 558
           DDWLPQTGVPYTSGLT+
Sbjct: 542 DDWLPQTGVPYTSGLTE 558

BLAST of CmUC09G165140.1 vs. NCBI nr
Match: XP_004136013.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucumis sativus] >KGN44976.1 hypothetical protein Csa_016133 [Cucumis sativus])

HSP 1 Score: 1026.5 bits (2653), Expect = 8.3e-296
Identity = 521/560 (93.04%), Postives = 536/560 (95.71%), Query Frame = 0

Query: 1   MAARTT-TAAAFY-FLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRS 60
           MAA TT TAAAFY  LILISLYI+TSA+PTGSVCSST DPSYCKS LPNQTGNVYSYGRS
Sbjct: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSR 120
           SFRKSLSSSQKFLRLVEK+LRSRSSLTVPAVRALEDC LLAGLNIDYL TSF+ VN+TSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTVNTTSR 120

Query: 121 TLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFT 180
            L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFFT
Sbjct: 121 VLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDDGG-DDQIA 240
           KGWVPKKKK+PTWKAAGRQG FRNGRMSLKMSSRT+AIYE ATRRNLLQ DDGG DDQI 
Sbjct: 181 KGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGDDDQIK 240

Query: 241 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMM 300
           VRDIVVVSQDGSGNFTTINEAIAAA NNSAP+DGYFLIFVSAG+YEEYV +AKNKRYLMM
Sbjct: 241 VRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNKRYLMM 300

Query: 301 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360
           IGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360

Query: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420
           DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ
Sbjct: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480
           FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNS AGVKTFLGRPWKEYSRTVYMQSFM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSDAGVKTFLGRPWKEYSRTVYMQSFM 480

Query: 481 DDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540
           DDLI+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERV W GFHLINDTDAGNFTAGNF
Sbjct: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWAGFHLINDTDAGNFTAGNF 540

Query: 541 VLADDWLPQTGVPYTSGLTD 558
           VLADDWLPQTGVPY SGLT+
Sbjct: 541 VLADDWLPQTGVPYDSGLTE 560

BLAST of CmUC09G165140.1 vs. NCBI nr
Match: KAG7014346.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1020.0 bits (2636), Expect = 7.7e-294
Identity = 512/562 (91.10%), Postives = 532/562 (94.66%), Query Frame = 0

Query: 1   MAARTTTAAAFYFLILISLY-----ISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSY 60
           MAA   T AAFY L+LISLY     ISTSAAPTGSVCSS  DPSYCKS LPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNS 120
           GRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFR VNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLA 180
           TS TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPLAADTKLYS+SLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDDGGDDQ 240
           FFTKGWVPKKKKKPTW +AGRQG F NGRMSLKMSSRTKAIYETATRRNLLQ DDGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYL 300
           I+VRDIVVVSQDGSGNFTTINEAIAAAPNNSAP+DGYFLIFV AG+YEEYV+IAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
            ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLI+P GWRAW+GDFALNT YYAEFGNFGPGSNT+ERVNW GFHLI+DTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 558
           NF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of CmUC09G165140.1 vs. NCBI nr
Match: KAG6575804.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1019.6 bits (2635), Expect = 1.0e-293
Identity = 512/562 (91.10%), Postives = 532/562 (94.66%), Query Frame = 0

Query: 1   MAARTTTAAAFYFLILISLY-----ISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSY 60
           MAA   T AAFY L+LISLY     ISTSAAPTGSVCSS  DPSYCKS LPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNS 120
           GRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFR VNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLA 180
           TS TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPLAADTKLYS+SLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDDGGDDQ 240
           FFTKGWVPKKKKKPTW +AGRQG F NGRMSLKMSSRTKAIYETATRRNLLQ +DGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYL 300
           I+VRDIVVVSQDGSGNFTTINEAIAAAPNNSAP+DGYFLIFV AG+YEEYV+IAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
            ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLI+P GWRAW+GDFALNT YYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 558
           NF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of CmUC09G165140.1 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 3.2e-202
Identity = 360/574 (62.72%), Postives = 433/574 (75.44%), Query Frame = 0

Query: 12  YFLILISLYI------STSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLS 71
           + LI +S ++      S + + + ++C +T DP YCKSV P+  GNV  YG  S RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 72  SSQKFLRLVEKYLRSRSSLTVPAV-RALEDCQLLAGLNIDYLTTSFRAVNSTS-----RT 131
            S+KF+R V++Y++  + L+ PAV RAL+DC+ LAGL +DYL TSF  VN TS     + 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 132 LPEMKADDVQSLLSAILTNQQTCLDGIKATA---GSWSLKNGLSLPLAADTKLYSLSLAF 191
           L   KADD+Q+LLSA LTN+QTCL+G+   A    +W+++ G++LPL  DTKL  +SLA 
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 192 FTKGWVPKKKKKP--TWKAAGRQGR---------FRNGRMSLKMSSRTKAIYETATRRNL 251
           FTKGWVPKKKK+    W A  R G          FRNG + LKM+ +TKA+YE+ +RR L
Sbjct: 186 FTKGWVPKKKKRAGFAW-AQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKL 245

Query: 252 LQIDDGGD----DQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGI 311
              D  GD      + + DIV VSQDG+GNFT I  A+AAAPNN+  S G+FLI+V+AGI
Sbjct: 246 ADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGI 305

Query: 312 YEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRN 371
           YEEY+SIAKNKRY+MMIGDGINQTVVTGNRSVVDGWTTFNSATFAV  P FVAVN+TFRN
Sbjct: 306 YEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRN 365

Query: 372 TAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAV 431
           TAG  KHQAVA+R+GAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAV
Sbjct: 366 TAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAV 425

Query: 432 VFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLG 491
           VFQNCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  +S   VKT+LG
Sbjct: 426 VFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLG 485

Query: 492 RPWKEYSRTVYMQSFMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGF 551
           RPWKEYSRTVYMQS++D  ++P GWR WNGDFAL+T YYAE+ N GPGSNT+ RV WPG+
Sbjct: 486 RPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGY 545

Query: 552 HLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 556
           H+IN TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 546 HVINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of CmUC09G165140.1 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 684.5 bits (1765), Expect = 1.0e-195
Identity = 354/562 (62.99%), Postives = 433/562 (77.05%), Query Frame = 0

Query: 13  FLILISLYI-STSAAPTGS---VCSSTRDPSYCKSVLPNQT-GNVYSYGRSSFRKSLSSS 72
           FL L +L+I S +  P+ S   +C +T DP +CKSV P  + G+V  YGR S RKSL+ S
Sbjct: 12  FLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQS 71

Query: 73  QKFLRLVEKYL-RSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST--SRTLPEMK 132
           +KF R +++YL R+ + L+  AV AL+DC+ LA L  DYL TSF  VN T  S+TL   K
Sbjct: 72  RKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSK 131

Query: 133 ADDVQSLLSAILTNQQTCLDGIK-ATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVP 192
           AD++Q+LLSA LTN+QTCLDGI  A + SW+++NG++LPL  DTKL+S+SLA FTKGWVP
Sbjct: 132 ADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVP 191

Query: 193 KKKK----------KPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDDGGD 252
           KKKK          K T         FRNG + LKM+  T+A+YE+ +RR L   DD   
Sbjct: 192 KKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-DDNDV 251

Query: 253 DQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKR 312
           + + V DIV V+Q+G+GNFTTI EA+ +APN +  + GYF+I+V++G+YEE V IAKNKR
Sbjct: 252 NTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKR 311

Query: 313 YLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAV 372
           YLMMIGDGIN+TVVTGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KHQAVA+
Sbjct: 312 YLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAM 371

Query: 373 RNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLP 432
           R+ ADLS FY CSFEAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+YPR P
Sbjct: 372 RSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQP 431

Query: 433 MSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYM 492
           M NQFNAITAQGRTDPNQNTG SI+NC I  ADDL  +S   VKT+LGRPWKEYSRTV+M
Sbjct: 432 MQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYSRTVFM 491

Query: 493 QSFMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFT 552
           QS++D++++P GWR WNGDFAL+T YYAE+ N G GS+T++RV WPG+H+IN TDA NFT
Sbjct: 492 QSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFT 551

Query: 553 AGNFVLADDWLPQTGVPYTSGL 556
             NF+L D W+ Q+GVPY SGL
Sbjct: 552 VENFLLGDGWMVQSGVPYISGL 571

BLAST of CmUC09G165140.1 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 655.6 bits (1690), Expect = 5.0e-187
Identity = 324/537 (60.34%), Postives = 410/537 (76.35%), Query Frame = 0

Query: 24  SAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLRSRSS 83
           +A    ++C    DPSYC+SVLPNQ G++YSYGR S R+SLS +++F+ +++  L  +  
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 84  LTVPA-VRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQSLLSAILTNQQTC 143
           +   + V ALEDC+ LA L +DYL +S +  +ST +TL   +A+DV + LSA +TN+QTC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 144 LDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKK-KPTWKAAGRQGR-- 203
           L+G+K+TA     +NGLS  L  DTKLY +SLA F+KGWVP++++ +P W+   R  +  
Sbjct: 151 LEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 204 -FRNGRMSLKMSSRTKAIYETATRRNLLQIDDGGDDQIAVRDIVVVSQDGSGNFTTINEA 263
            FRNG++ LKM+ R +A+Y T TRR LLQ D    D + V DIV V Q+G+GNFTTIN A
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQNGTGNFTTINAA 270

Query: 264 IAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWT 323
           IAAAPN +  S+GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTV+TGNRSVVDGWT
Sbjct: 271 IAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWT 330

Query: 324 TFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHS 383
           TFNSATF + GP F+ VN+T RNTAG  K QAVA+R+G DLS FY CSFEAYQDTLYTHS
Sbjct: 331 TFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHS 390

Query: 384 LRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIY 443
           LRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q N +TAQGRTDPNQNTGT+I+
Sbjct: 391 LRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIH 450

Query: 444 NCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLIDPEGWRAWNGDFALNTS 503
            C I  ADDLA  S   VKT+LGRPWKEYSRTV MQ+++D  ++P GW AW+GDFAL+T 
Sbjct: 451 GCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTL 510

Query: 504 YYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 556
           YYAE+ N GPGS+T+ RV WPG+H+IN TDA NFT  NF++ + W+ QTGVP+  GL
Sbjct: 511 YYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of CmUC09G165140.1 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 3.1e-136
Identity = 261/550 (47.45%), Postives = 350/550 (63.64%), Query Frame = 0

Query: 24  SAAPTGSVCSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLR- 83
           S  P    C ST  P  C+++L    +   + Y YG+ + ++ L  +++  +++ ++ + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 84  ---SRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQSLLSAIL 143
                 + TV  V A+ DC  LA L+++YL T    + + +  +     D V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKA-AELMTAALVDRVTSLLGGVV 199

Query: 144 TNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKKKPTWKAAGR 203
           TNQQTCLDG+      ++    +  PL   T+LYS+SL     G V     +   +  G 
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSL-----GLVSHALNRNLKRYKGS 259

Query: 204 QGR-FRNGRMSLK--MSSRTKAIYETATR--------RNLLQIDDGGDDQIAVRDIVVVS 263
           +G+ F  G   ++  + +  K + +T  +        RNL ++ +     I VR+ V V 
Sbjct: 260 KGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVG 319

Query: 264 QDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGINQT 323
              + NF TI EA+AAAPN++ P  GYF+I+  AG+YEEYV I+  KR +M+IGDGIN+T
Sbjct: 320 PYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKT 379

Query: 324 VVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLC 383
           +++GN S +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY C
Sbjct: 380 IISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRC 439

Query: 384 SFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQG 443
           SFE YQDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PM+NQ NA+TA G
Sbjct: 440 SFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHG 499

Query: 444 RTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLIDPEG 503
           RTDPNQ TG SI NC I AA DLA +      TFLGRPWK YSRTVY+QS++ D++ P G
Sbjct: 500 RTDPNQKTGISIINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVG 559

Query: 504 WRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLP 556
           W  WNG   L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLP
Sbjct: 560 WLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLP 619

BLAST of CmUC09G165140.1 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 6.9e-136
Identity = 261/556 (46.94%), Postives = 354/556 (63.67%), Query Frame = 0

Query: 18  SLYISTSAAPTGSVCSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLV 77
           SL  S S +P+   C ST  P  C+++L    +   + Y YG+ + ++ L  + +  +++
Sbjct: 70  SLPPSQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVI 129

Query: 78  EKYLR----SRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQS 137
             Y R       S T   + A+ DC  L+ L+++YL T    +  T++ +     + V S
Sbjct: 130 TSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTEL-KTAQVMTAALVEHVNS 189

Query: 138 LLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKKKPT 197
           LLS ++TNQQTCLDG+      ++    +  P+   T+LYS+SL     G V     +  
Sbjct: 190 LLSGVVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISL-----GLVSHALNRNL 249

Query: 198 WKAAGRQGRFRNGRMSL---KMSSRTKAIYETA--------TRRNLLQIDDGGDDQIAVR 257
            +    +G+   G  S     + +  K + +T         T RNL ++ +     I V 
Sbjct: 250 KRFKASKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVS 309

Query: 258 DIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIG 317
             V+V    S NFTTI +AIAAAPNN+ P DGYF+I+   G+YEEY+ +  NK+ LM++G
Sbjct: 310 KAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMG 369

Query: 318 DGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADL 377
           DGIN+T++TGN +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ 
Sbjct: 370 DGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEG 429

Query: 378 STFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFN 437
           S+FY CSFE YQDTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PM+ Q N
Sbjct: 430 SSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKN 489

Query: 438 AITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDD 497
           AITA GR DPNQNTG SI NC I AA DLA    + + TFLGRPWK YSRTV+MQS++ D
Sbjct: 490 AITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISD 549

Query: 498 LIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVL 556
           ++ P GW  WNG   L+T YY E+ NFGPG+NT++RV W G++L+N  +A NFT  NF +
Sbjct: 550 IVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTM 609

BLAST of CmUC09G165140.1 vs. ExPASy TrEMBL
Match: A0A5D3D3H4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001320 PE=3 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 2.2e-302
Identity = 526/557 (94.43%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 3   ARTTTAAAFYFLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRK 62
           A  TTAAAFY LILISLYI+TSA+PTGSVCSST DPSYCKS LPNQTGNVYSYGRSSFRK
Sbjct: 2   AAGTTAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRK 61

Query: 63  SLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPE 122
           SLSSSQKFLRLVEK+LRSRSSLTV AVRALEDCQLLAGLNIDYL TSF+AVN+TSR L E
Sbjct: 62  SLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRALTE 121

Query: 123 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWV 182
           MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFFTKGWV
Sbjct: 122 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWV 181

Query: 183 PKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDD--GGDDQIAVRD 242
           PKKKKKPTWKAAGRQG FRNGRMSLKMSSRT+AIYETATRRNLLQ DD  G DDQIAVRD
Sbjct: 182 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIAVRD 241

Query: 243 IVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGD 302
           IVVVSQDG+GNFTTINEAIAAAPNNSAP+DGYFLIFVSAG+YEEYVSIAKNKRYLMMIGD
Sbjct: 242 IVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 301

Query: 303 GINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLS 362
           GINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RNGADLS
Sbjct: 302 GINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGADLS 361

Query: 363 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 422
           TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA
Sbjct: 362 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 421

Query: 423 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 482
           ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL
Sbjct: 422 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 481

Query: 483 IDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 542
           I+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA
Sbjct: 482 INPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 541

Query: 543 DDWLPQTGVPYTSGLTD 558
           DDWLPQTGVPYTSGLT+
Sbjct: 542 DDWLPQTGVPYTSGLTE 558

BLAST of CmUC09G165140.1 vs. ExPASy TrEMBL
Match: A0A1S3BSI2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 2.2e-302
Identity = 526/557 (94.43%), Postives = 541/557 (97.13%), Query Frame = 0

Query: 3   ARTTTAAAFYFLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRK 62
           A  TTAAAFY LILISLYI+TSA+PTGSVCSST DPSYCKS LPNQTGNVYSYGRSSFRK
Sbjct: 2   AAGTTAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRK 61

Query: 63  SLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPE 122
           SLSSSQKFLRLVEK+LRSRSSLTV AVRALEDCQLLAGLNIDYL TSF+AVN+TSR L E
Sbjct: 62  SLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRALTE 121

Query: 123 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWV 182
           MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFFTKGWV
Sbjct: 122 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWV 181

Query: 183 PKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDD--GGDDQIAVRD 242
           PKKKKKPTWKAAGRQG FRNGRMSLKMSSRT+AIYETATRRNLLQ DD  G DDQIAVRD
Sbjct: 182 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIAVRD 241

Query: 243 IVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGD 302
           IVVVSQDG+GNFTTINEAIAAAPNNSAP+DGYFLIFVSAG+YEEYVSIAKNKRYLMMIGD
Sbjct: 242 IVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 301

Query: 303 GINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLS 362
           GINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RNGADLS
Sbjct: 302 GINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGADLS 361

Query: 363 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 422
           TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA
Sbjct: 362 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 421

Query: 423 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 482
           ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL
Sbjct: 422 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 481

Query: 483 IDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 542
           I+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA
Sbjct: 482 INPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 541

Query: 543 DDWLPQTGVPYTSGLTD 558
           DDWLPQTGVPYTSGLT+
Sbjct: 542 DDWLPQTGVPYTSGLTE 558

BLAST of CmUC09G165140.1 vs. ExPASy TrEMBL
Match: A0A0A0KB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405820 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 4.0e-296
Identity = 521/560 (93.04%), Postives = 536/560 (95.71%), Query Frame = 0

Query: 1   MAARTT-TAAAFY-FLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRS 60
           MAA TT TAAAFY  LILISLYI+TSA+PTGSVCSST DPSYCKS LPNQTGNVYSYGRS
Sbjct: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSR 120
           SFRKSLSSSQKFLRLVEK+LRSRSSLTVPAVRALEDC LLAGLNIDYL TSF+ VN+TSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTVNTTSR 120

Query: 121 TLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFT 180
            L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFFT
Sbjct: 121 VLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDDGG-DDQIA 240
           KGWVPKKKK+PTWKAAGRQG FRNGRMSLKMSSRT+AIYE ATRRNLLQ DDGG DDQI 
Sbjct: 181 KGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGDDDQIK 240

Query: 241 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMM 300
           VRDIVVVSQDGSGNFTTINEAIAAA NNSAP+DGYFLIFVSAG+YEEYV +AKNKRYLMM
Sbjct: 241 VRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNKRYLMM 300

Query: 301 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360
           IGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360

Query: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420
           DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ
Sbjct: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480
           FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNS AGVKTFLGRPWKEYSRTVYMQSFM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSDAGVKTFLGRPWKEYSRTVYMQSFM 480

Query: 481 DDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540
           DDLI+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERV W GFHLINDTDAGNFTAGNF
Sbjct: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWAGFHLINDTDAGNFTAGNF 540

Query: 541 VLADDWLPQTGVPYTSGLTD 558
           VLADDWLPQTGVPY SGLT+
Sbjct: 541 VLADDWLPQTGVPYDSGLTE 560

BLAST of CmUC09G165140.1 vs. ExPASy TrEMBL
Match: A0A6J1GQE6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 1.4e-293
Identity = 511/562 (90.93%), Postives = 531/562 (94.48%), Query Frame = 0

Query: 1   MAARTTTAAAFYFLILISLY-----ISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSY 60
           MAA   T AAFY L+LISLY     ISTSAAPTGSVCSS  DPSYCKS LPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNS 120
           GRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFR VNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLA 180
           TS TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPLAADTKLYS+SLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDDGGDDQ 240
           FFTKGWVPKKKKKPTW +AGRQG F NGRMSLKMSSRTKAIYETATRRNLLQ +DGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFNNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYL 300
           I+VRDIVVVSQDGSGNFTTINEAIAAAPNNSAP+DGYFLIFV AG+YEEYV+IAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRN AGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNAAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
            ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLI+P GWRAW+GDFALNT YYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 558
           NF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of CmUC09G165140.1 vs. ExPASy TrEMBL
Match: A0A6J1JVJ5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 3.9e-291
Identity = 506/562 (90.04%), Postives = 529/562 (94.13%), Query Frame = 0

Query: 1   MAARTTTAAAFYFLILISLY-----ISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSY 60
           MAA   T  AFY+L+LISLY     ISTSAAPTGSVCSS  DPSYCKS LPNQTGNVYSY
Sbjct: 1   MAAAPPTTPAFYYLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNS 120
           GRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVR+LEDCQLLAGLNIDYLTTSFR VNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRSLEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLA 180
           TS TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPL ADTKLYS+SLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLDADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDDGGDDQ 240
           FFTKGWVPKKKKK TW +AGRQG F NGRMS KMSSRTKAIYE ATRRNLLQ DDGGDDQ
Sbjct: 181 FFTKGWVPKKKKKQTWTSAGRQGGFSNGRMSFKMSSRTKAIYEMATRRNLLQTDDGGDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYL 300
           I+VRDIVVVSQDGSGNFTTINEAIAAAPNNSAP+DGYFLIFV AG+YEEYV+IAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
            ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANN+GA V TFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FM+DLI+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNFTAG
Sbjct: 481 FMNDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 558
           NF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of CmUC09G165140.1 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 706.1 bits (1821), Expect = 2.3e-203
Identity = 360/574 (62.72%), Postives = 433/574 (75.44%), Query Frame = 0

Query: 12  YFLILISLYI------STSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLS 71
           + LI +S ++      S + + + ++C +T DP YCKSV P+  GNV  YG  S RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 72  SSQKFLRLVEKYLRSRSSLTVPAV-RALEDCQLLAGLNIDYLTTSFRAVNSTS-----RT 131
            S+KF+R V++Y++  + L+ PAV RAL+DC+ LAGL +DYL TSF  VN TS     + 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 132 LPEMKADDVQSLLSAILTNQQTCLDGIKATA---GSWSLKNGLSLPLAADTKLYSLSLAF 191
           L   KADD+Q+LLSA LTN+QTCL+G+   A    +W+++ G++LPL  DTKL  +SLA 
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 192 FTKGWVPKKKKKP--TWKAAGRQGR---------FRNGRMSLKMSSRTKAIYETATRRNL 251
           FTKGWVPKKKK+    W A  R G          FRNG + LKM+ +TKA+YE+ +RR L
Sbjct: 186 FTKGWVPKKKKRAGFAW-AQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKL 245

Query: 252 LQIDDGGD----DQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGI 311
              D  GD      + + DIV VSQDG+GNFT I  A+AAAPNN+  S G+FLI+V+AGI
Sbjct: 246 ADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGI 305

Query: 312 YEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRN 371
           YEEY+SIAKNKRY+MMIGDGINQTVVTGNRSVVDGWTTFNSATFAV  P FVAVN+TFRN
Sbjct: 306 YEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRN 365

Query: 372 TAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAV 431
           TAG  KHQAVA+R+GAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAV
Sbjct: 366 TAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAV 425

Query: 432 VFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLG 491
           VFQNCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  +S   VKT+LG
Sbjct: 426 VFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLG 485

Query: 492 RPWKEYSRTVYMQSFMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGF 551
           RPWKEYSRTVYMQS++D  ++P GWR WNGDFAL+T YYAE+ N GPGSNT+ RV WPG+
Sbjct: 486 RPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGY 545

Query: 552 HLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 556
           H+IN TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 546 HVINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of CmUC09G165140.1 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 684.5 bits (1765), Expect = 7.2e-197
Identity = 354/562 (62.99%), Postives = 433/562 (77.05%), Query Frame = 0

Query: 13  FLILISLYI-STSAAPTGS---VCSSTRDPSYCKSVLPNQT-GNVYSYGRSSFRKSLSSS 72
           FL L +L+I S +  P+ S   +C +T DP +CKSV P  + G+V  YGR S RKSL+ S
Sbjct: 12  FLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQS 71

Query: 73  QKFLRLVEKYL-RSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST--SRTLPEMK 132
           +KF R +++YL R+ + L+  AV AL+DC+ LA L  DYL TSF  VN T  S+TL   K
Sbjct: 72  RKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSK 131

Query: 133 ADDVQSLLSAILTNQQTCLDGIK-ATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVP 192
           AD++Q+LLSA LTN+QTCLDGI  A + SW+++NG++LPL  DTKL+S+SLA FTKGWVP
Sbjct: 132 ADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVP 191

Query: 193 KKKK----------KPTWKAAGRQGRFRNGRMSLKMSSRTKAIYETATRRNLLQIDDGGD 252
           KKKK          K T         FRNG + LKM+  T+A+YE+ +RR L   DD   
Sbjct: 192 KKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-DDNDV 251

Query: 253 DQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKR 312
           + + V DIV V+Q+G+GNFTTI EA+ +APN +  + GYF+I+V++G+YEE V IAKNKR
Sbjct: 252 NTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKR 311

Query: 313 YLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAV 372
           YLMMIGDGIN+TVVTGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KHQAVA+
Sbjct: 312 YLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAM 371

Query: 373 RNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLP 432
           R+ ADLS FY CSFEAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+YPR P
Sbjct: 372 RSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQP 431

Query: 433 MSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYM 492
           M NQFNAITAQGRTDPNQNTG SI+NC I  ADDL  +S   VKT+LGRPWKEYSRTV+M
Sbjct: 432 MQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYSRTVFM 491

Query: 493 QSFMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFT 552
           QS++D++++P GWR WNGDFAL+T YYAE+ N G GS+T++RV WPG+H+IN TDA NFT
Sbjct: 492 QSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFT 551

Query: 553 AGNFVLADDWLPQTGVPYTSGL 556
             NF+L D W+ Q+GVPY SGL
Sbjct: 552 VENFLLGDGWMVQSGVPYISGL 571

BLAST of CmUC09G165140.1 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 655.6 bits (1690), Expect = 3.6e-188
Identity = 324/537 (60.34%), Postives = 410/537 (76.35%), Query Frame = 0

Query: 24  SAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLRSRSS 83
           +A    ++C    DPSYC+SVLPNQ G++YSYGR S R+SLS +++F+ +++  L  +  
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 84  LTVPA-VRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQSLLSAILTNQQTC 143
           +   + V ALEDC+ LA L +DYL +S +  +ST +TL   +A+DV + LSA +TN+QTC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 144 LDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKK-KPTWKAAGRQGR-- 203
           L+G+K+TA     +NGLS  L  DTKLY +SLA F+KGWVP++++ +P W+   R  +  
Sbjct: 151 LEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 204 -FRNGRMSLKMSSRTKAIYETATRRNLLQIDDGGDDQIAVRDIVVVSQDGSGNFTTINEA 263
            FRNG++ LKM+ R +A+Y T TRR LLQ D    D + V DIV V Q+G+GNFTTIN A
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQNGTGNFTTINAA 270

Query: 264 IAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWT 323
           IAAAPN +  S+GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTV+TGNRSVVDGWT
Sbjct: 271 IAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWT 330

Query: 324 TFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHS 383
           TFNSATF + GP F+ VN+T RNTAG  K QAVA+R+G DLS FY CSFEAYQDTLYTHS
Sbjct: 331 TFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHS 390

Query: 384 LRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIY 443
           LRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q N +TAQGRTDPNQNTGT+I+
Sbjct: 391 LRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIH 450

Query: 444 NCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLIDPEGWRAWNGDFALNTS 503
            C I  ADDLA  S   VKT+LGRPWKEYSRTV MQ+++D  ++P GW AW+GDFAL+T 
Sbjct: 451 GCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTL 510

Query: 504 YYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 556
           YYAE+ N GPGS+T+ RV WPG+H+IN TDA NFT  NF++ + W+ QTGVP+  GL
Sbjct: 511 YYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of CmUC09G165140.1 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 486.9 bits (1252), Expect = 2.2e-137
Identity = 261/550 (47.45%), Postives = 350/550 (63.64%), Query Frame = 0

Query: 24  SAAPTGSVCSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLR- 83
           S  P    C ST  P  C+++L    +   + Y YG+ + ++ L  +++  +++ ++ + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 84  ---SRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQSLLSAIL 143
                 + TV  V A+ DC  LA L+++YL T    + + +  +     D V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKA-AELMTAALVDRVTSLLGGVV 199

Query: 144 TNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKKKPTWKAAGR 203
           TNQQTCLDG+      ++    +  PL   T+LYS+SL     G V     +   +  G 
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSL-----GLVSHALNRNLKRYKGS 259

Query: 204 QGR-FRNGRMSLK--MSSRTKAIYETATR--------RNLLQIDDGGDDQIAVRDIVVVS 263
           +G+ F  G   ++  + +  K + +T  +        RNL ++ +     I VR+ V V 
Sbjct: 260 KGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVG 319

Query: 264 QDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGINQT 323
              + NF TI EA+AAAPN++ P  GYF+I+  AG+YEEYV I+  KR +M+IGDGIN+T
Sbjct: 320 PYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKT 379

Query: 324 VVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLC 383
           +++GN S +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY C
Sbjct: 380 IISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRC 439

Query: 384 SFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQG 443
           SFE YQDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PM+NQ NA+TA G
Sbjct: 440 SFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHG 499

Query: 444 RTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLIDPEG 503
           RTDPNQ TG SI NC I AA DLA +      TFLGRPWK YSRTVY+QS++ D++ P G
Sbjct: 500 RTDPNQKTGISIINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVG 559

Query: 504 WRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLP 556
           W  WNG   L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLP
Sbjct: 560 WLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLP 619

BLAST of CmUC09G165140.1 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 485.7 bits (1249), Expect = 4.9e-137
Identity = 261/556 (46.94%), Postives = 354/556 (63.67%), Query Frame = 0

Query: 18  SLYISTSAAPTGSVCSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLV 77
           SL  S S +P+   C ST  P  C+++L    +   + Y YG+ + ++ L  + +  +++
Sbjct: 70  SLPPSQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVI 129

Query: 78  EKYLR----SRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQS 137
             Y R       S T   + A+ DC  L+ L+++YL T    +  T++ +     + V S
Sbjct: 130 TSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTEL-KTAQVMTAALVEHVNS 189

Query: 138 LLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKKKPT 197
           LLS ++TNQQTCLDG+      ++    +  P+   T+LYS+SL     G V     +  
Sbjct: 190 LLSGVVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISL-----GLVSHALNRNL 249

Query: 198 WKAAGRQGRFRNGRMSL---KMSSRTKAIYETA--------TRRNLLQIDDGGDDQIAVR 257
            +    +G+   G  S     + +  K + +T         T RNL ++ +     I V 
Sbjct: 250 KRFKASKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVS 309

Query: 258 DIVVVSQDGSGNFTTINEAIAAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIG 317
             V+V    S NFTTI +AIAAAPNN+ P DGYF+I+   G+YEEY+ +  NK+ LM++G
Sbjct: 310 KAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMG 369

Query: 318 DGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADL 377
           DGIN+T++TGN +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ 
Sbjct: 370 DGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEG 429

Query: 378 STFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFN 437
           S+FY CSFE YQDTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PM+ Q N
Sbjct: 430 SSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKN 489

Query: 438 AITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDD 497
           AITA GR DPNQNTG SI NC I AA DLA    + + TFLGRPWK YSRTV+MQS++ D
Sbjct: 490 AITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISD 549

Query: 498 LIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVL 556
           ++ P GW  WNG   L+T YY E+ NFGPG+NT++RV W G++L+N  +A NFT  NF +
Sbjct: 550 IVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTM 609

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896831.14.3e-31096.26probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida][more]
XP_008451544.14.5e-30294.43PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Cucumis melo] >KA... [more]
XP_004136013.18.3e-29693.04probable pectinesterase/pectinesterase inhibitor 7 [Cucumis sativus] >KGN44976.1... [more]
KAG7014346.17.7e-29491.10putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
KAG6575804.11.0e-29391.10putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
Q9SRX43.2e-20262.72Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
Q8RXK71.0e-19562.99Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
O222565.0e-18760.34Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Q9FF773.1e-13647.45Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Q94CB16.9e-13646.94Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5D3D3H42.2e-30294.43Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00132... [more]
A0A1S3BSI22.2e-30294.43Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1[more]
A0A0A0KB124.0e-29693.04Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405820 PE=3 SV=1[more]
A0A6J1GQE61.4e-29390.93Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1[more]
A0A6J1JVJ53.9e-29190.04Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G02810.12.3e-20362.72Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.17.2e-19762.99Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.13.6e-18860.34Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.12.2e-13747.45Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.24.9e-13746.94Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 22..176
e-value: 3.0E-22
score: 89.9
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 30..175
e-value: 1.1E-16
score: 61.5
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 236..555
e-value: 8.7E-119
score: 398.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 28..179
e-value: 2.5E-20
score: 75.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 30..179
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 242..539
e-value: 2.5E-135
score: 450.0
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 13..555
NoneNo IPR availablePANTHERPTHR31707:SF294PECTINESTERASE/PECTINESTERASE INHIBITOR 7-RELATEDcoord: 13..555
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 30..178
e-value: 1.0173E-27
score: 106.754
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 388..397
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 237..554

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmUC09G165140CmUC09G165140gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmUC09G165140.1-exonCmUC09G165140.1-exon-CmU531Chr09:4547910..4548719exon
CmUC09G165140.1-exonCmUC09G165140.1-exon-CmU531Chr09:4549606..4550043exon
CmUC09G165140.1-exonCmUC09G165140.1-exon-CmU531Chr09:4551180..4551766exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmUC09G165140.1-three_prime_utrCmUC09G165140.1-three_prime_utr-CmU531Chr09:4547910..4548021three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmUC09G165140.1-cdsCmUC09G165140.1-cds-CmU531Chr09:4548022..4548719CDS
CmUC09G165140.1-cdsCmUC09G165140.1-cds-CmU531Chr09:4549606..4550043CDS
CmUC09G165140.1-cdsCmUC09G165140.1-cds-CmU531Chr09:4551180..4551717CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmUC09G165140.1-five_prime_utrCmUC09G165140.1-five_prime_utr-CmU531Chr09:4551718..4551766five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmUC09G165140.1CmUC09G165140.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity