CmUC02G040540.1 (mRNA) Watermelon (USVL531) v1

Overview


Name	CmUC02G040540.1
Type	mRNA
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	nicotianamine synthase-like
Location	CmU531Chr02: 28354566 .. 28356222 (-)
Sequence length	981
RNA-Seq Expression	CmUC02G040540.1
Synteny	CmUC02G040540.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTTGAAGAAGCCTCTCTCTTGCAAAAAGTCTGTGCTCTTTACAACCAAATCTCAAGCCTCGCTAGCCTCGAGCCTTCAAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCTCCACCTGCCCCTGGCCTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATTCGCCTTTGTGCTAAAGCTGAAGCTCTTCTTGAACTTCATTTCTCTTCCATTTTGGCTTCCTCTTTTGATAATCCCATTTCTAACTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTCTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTATCGTCGTGTCCCCGCTACCGTCGCGTTCCTTGGCTCTGGACCCCTCCCGCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTAACCGTTGTTGAATTAGTTATACCTATACTTATAGGTTTTATGGTTAGCTTTACATGCTTCTTGGATAAACATGAAATTTCATCTCGTAAAATTGATTAATCTATAATAACGAGAAAAATAACACATTTAGCGTATAAAAATTGTGAGGTTTCTCTATCTTTTAAAGAATGTGGGATCCTCAACGTGCCCCTTTGAGATGGTGTTTCTTTAGGTTTACCATTTTTGGGTTCAGATTTTTTTTTTTCCTTATGTTTCATAGACTTTGATACAAGATTAGATAAACATGAAGTTCTAACTCAAAATCAATTGGCAACAAGAGAGCAGTAACTCATTTATACTATGGGTCTCCTCATCTTTCCAATGTAAGATCCTCAATAAATATGTGGTGTGTTAGATTTTTTTTGTAGATTTGATTGGTTGCTTTGTCATTTTCTTCACTTGTGTGGCGACAGTATACGAATTTGAATATCTAAACCATATCTTTCTTGTGTTCAAGGAGGAATTGGATAGGATGGAATGAAGTTATTATTAACTCTACTATTTGTTAGTGTAGTCCCATGACTTATTAACATAAACTCTATGACTTGTTAACTTCTTGCACTGTAGGCATTCCAGTATCGAACAATTTATCAAACTTAACAACTTCAAACTAAACAATTTCCGTTGTTAATAGGAACTATTTTCCACAACTTTGACATTGATCCAGCTGCAAACACCATGGCTAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTCCACACAACAGATGTAATGGAAGTGACAAAGGGATTAAAAGATTATGAAGTAGTTTTCTTAGCAGCATTGGTAGGATTAGAAGAGGAAGAAAAAGGAAGAGTTCTAAGGCATTTGGGGAAACATATGGCAGCTGGTGCTTATTTGATGCTAAGAAGTGCACATGGAGCTAGAGCATTTTTATATCCTGTTGTTGATATTTGTAACGTTGAAGCTTCTGGATTTGAGATTTTATCTGTGTTCCATCCCACTGATGAAGTTATAAACTCTGTTGTTATTGCAAGGAAGAAACTAGTGTTTGATCGAAACTCGATCGAGGATGAGGATGACGACGAGGACGACGAGAAGCAAGGTATATTAGTTAATACCTCATCTATTGTGTTGCCTGAGAAATGCTCTGGGTTTAATGGCTTTAGTCCCATGATTGAAGAGCAACTTTCTTGA

mRNA sequence

ATGGAATTTGAAGAAGCCTCTCTCTTGCAAAAAGTCTGTGCTCTTTACAACCAAATCTCAAGCCTCGCTAGCCTCGAGCCTTCAAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCTCCACCTGCCCCTGGCCTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATTCGCCTTTGTGCTAAAGCTGAAGCTCTTCTTGAACTTCATTTCTCTTCCATTTTGGCTTCCTCTTTTGATAATCCCATTTCTAACTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTCTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTATCGTCGTGTCCCCGCTACCGTCGCGTTCCTTGGCTCTGGACCCCTCCCGCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGAACTATTTTCCACAACTTTGACATTGATCCAGCTGCAAACACCATGGCTAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTCCACACAACAGATGTAATGGAAGTGACAAAGGGATTAAAAGATTATGAAGTAGTTTTCTTAGCAGCATTGGTAGGATTAGAAGAGGAAGAAAAAGGAAGAGTTCTAAGGCATTTGGGGAAACATATGGCAGCTGGTGCTTATTTGATGCTAAGAAGTGCACATGGAGCTAGAGCATTTTTATATCCTGTTGTTGATATTTGTAACGTTGAAGCTTCTGGATTTGAGATTTTATCTGTGTTCCATCCCACTGATGAAGTTATAAACTCTGTTGTTATTGCAAGGAAGAAACTAGTGTTTGATCGAAACTCGATCGAGGATGAGGATGACGACGAGGACGACGAGAAGCAAGGTATATTAGTTAATACCTCATCTATTGTGTTGCCTGAGAAATGCTCTGGGTTTAATGGCTTTAGTCCCATGATTGAAGAGCAACTTTCTTGA

Coding sequence (CDS)

ATGGAATTTGAAGAAGCCTCTCTCTTGCAAAAAGTCTGTGCTCTTTACAACCAAATCTCAAGCCTCGCTAGCCTCGAGCCTTCAAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCTCCACCTGCCCCTGGCCTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATTCGCCTTTGTGCTAAAGCTGAAGCTCTTCTTGAACTTCATTTCTCTTCCATTTTGGCTTCCTCTTTTGATAATCCCATTTCTAACTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTCTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTATCGTCGTGTCCCCGCTACCGTCGCGTTCCTTGGCTCTGGACCCCTCCCGCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGAACTATTTTCCACAACTTTGACATTGATCCAGCTGCAAACACCATGGCTAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTCCACACAACAGATGTAATGGAAGTGACAAAGGGATTAAAAGATTATGAAGTAGTTTTCTTAGCAGCATTGGTAGGATTAGAAGAGGAAGAAAAAGGAAGAGTTCTAAGGCATTTGGGGAAACATATGGCAGCTGGTGCTTATTTGATGCTAAGAAGTGCACATGGAGCTAGAGCATTTTTATATCCTGTTGTTGATATTTGTAACGTTGAAGCTTCTGGATTTGAGATTTTATCTGTGTTCCATCCCACTGATGAAGTTATAAACTCTGTTGTTATTGCAAGGAAGAAACTAGTGTTTGATCGAAACTCGATCGAGGATGAGGATGACGACGAGGACGACGAGAAGCAAGGTATATTAGTTAATACCTCATCTATTGTGTTGCCTGAGAAATGCTCTGGGTTTAATGGCTTTAGTCCCATGATTGAAGAGCAACTTTCTTGA

Protein sequence

MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDEDDDEDDEKQGILVNTSSIVLPEKCSGFNGFSPMIEEQLS

Homology

[Close]

BLAST of CmUC02G040540.1 vs. NCBI nr
Match: XP_038890564.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 572.4 bits (1474), Expect = 2.5e-159
Identity = 297/328 (90.55%), Postives = 313/328 (95.43%), Query Frame = 0


Query: 1   MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAP-GLDIASLSQPLQ 60
           MEFEEA LLQK+ ALY+QISSL SL PSKNVDTLFTQLVLTCSPPAP G DIASLSQPLQ
Sbjct: 1   MEFEEAPLLQKISALYHQISSLTSLNPSKNVDTLFTQLVLTCSPPAPAGFDIASLSQPLQ 60

Query: 61  AMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHY 120
           AMRAHLIRLCAKAEALLELHFSSILASSF NPISNLSIFPYYSNYLKLSLLEFDILRSH 
Sbjct: 61  AMRAHLIRLCAKAEALLELHFSSILASSFRNPISNLSIFPYYSNYLKLSLLEFDILRSHC 120

Query: 121 RRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMI 180
           RRVPA++AFLGSGPLPLSSIV+ASIHLKGTIFHNFD+DP AN+MASQLVCSDPDLSQRMI
Sbjct: 121 RRVPASIAFLGSGPLPLSSIVMASIHLKGTIFHNFDLDPTANSMASQLVCSDPDLSQRMI 180

Query: 181 FHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAF 240
           FHTTDVM VT GLKDYEVVFLAALVGLEEEEKGRVL+HLGKHMAAGAYLMLRSAHGARAF
Sbjct: 181 FHTTDVMNVTNGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGAYLMLRSAHGARAF 240

Query: 241 LYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDEDDDED-DEKQGI 300
           LYPVVDIC+VEASGFEILSVFHPTDEVINSVVIARKK +FDRNS+EDEDDD++  EK+GI
Sbjct: 241 LYPVVDICDVEASGFEILSVFHPTDEVINSVVIARKKALFDRNSVEDEDDDDNAAEKRGI 300

Query: 301 LVNTSSIVLPEKCSGFNGFSPMIEEQLS 327
           LV+TSSIVL EKCSGFNGFSPM+EEQLS
Sbjct: 301 LVDTSSIVLAEKCSGFNGFSPMVEEQLS 328

[Close]

BLAST of CmUC02G040540.1 vs. NCBI nr
Match: KAA0048069.1 (putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa] >TYJ96467.1 putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 553.1 bits (1424), Expect = 1.6e-153
Identity = 290/330 (87.88%), Postives = 311/330 (94.24%), Query Frame = 0


Query: 2   EFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCS---PPAP-GLDIASLSQP 61
           E +EA LLQKVCALYNQISSL+SL+P KNVDTLFTQLVLTCS   P AP G D+ASLSQP
Sbjct: 9   EQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQP 68

Query: 62  LQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRS 121
           LQAMRAHLI+LCAKAEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSLLEFDILRS
Sbjct: 69  LQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRS 128

Query: 122 HYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQR 181
           H RR+P  VAF+GSGPLPLSSIVLASIHLKGTIFHNFDIDP ANTMASQLVCSDPDLSQR
Sbjct: 129 HSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQR 188

Query: 182 MIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGAR 241
           MIFHT DVMEVTKGLKDYEVVFLAALVGLEEEEKGRVL+HLGKHMAAG+YLMLRSAHGAR
Sbjct: 189 MIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGAR 248

Query: 242 AFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDED-DDEDDEKQ 301
           AFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK+VFD++S+EDED D+E++EK 
Sbjct: 249 AFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDEDEEEEEKP 308

Query: 302 GILVNTSSIVLPEKCSGFNGFSPMIEEQLS 327
           GILV+TSSIVL EKCSGFNGF+P+IEEQLS
Sbjct: 309 GILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338

[Close]

BLAST of CmUC02G040540.1 vs. NCBI nr
Match: XP_004144750.1 (probable nicotianamine synthase 4 [Cucumis sativus] >KGN61000.1 hypothetical protein Csa_021149 [Cucumis sativus])

HSP 1 Score: 547.0 bits (1408), Expect = 1.1e-151
Identity = 289/332 (87.05%), Postives = 307/332 (92.47%), Query Frame = 0


Query: 1   MEFE---EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCS---PPAPGLDIASL 60
           MEFE   EA LL+KVCALYNQISSL+SL+P KNVDTLFTQLVLTCS   PP  G DIASL
Sbjct: 1   MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL 60

Query: 61  SQPLQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDI 120
           SQPL+AMRAHLI+LCA+AEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSLLEFDI
Sbjct: 61  SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI 120

Query: 121 LRSHYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDL 180
           LRSH RR+P  VAF+GSGPLPLSSIVLASIHLKGTIFHNFDIDP ANTMASQLVCSDPDL
Sbjct: 121 LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL 180

Query: 181 SQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAH 240
           SQRMIFHT DVMEVTKGLKDYEVVFLAALVGL EEEKGRVL+HLGKHMAAG+YLMLRSAH
Sbjct: 181 SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH 240

Query: 241 GARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDEDDDEDDE 300
           GARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK++FD++S EDED   +DE
Sbjct: 241 GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDED---EDE 300

Query: 301 KQGILVNTSSIVLPEKCSGFNGFSPMIEEQLS 327
           KQGILVNTSSIVL EKCSGFNGF+PMIEE LS
Sbjct: 301 KQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

[Close]

BLAST of CmUC02G040540.1 vs. NCBI nr
Match: XP_022946411.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 498.0 bits (1281), Expect = 6.0e-137
Identity = 264/321 (82.24%), Postives = 287/321 (89.41%), Query Frame = 0


Query: 5   EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAH 64
           E  LL++VCALY+QISSLASL+PSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMRAH
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 61

Query: 65  LIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPA 124
           LIRLCA AEALLE+HFSSILA SF+NPI NLSIFPYYSNYLKLSLLEFDIL SH RRVP+
Sbjct: 62  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 121

Query: 125 TVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTD 184
           +VAF+GSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA QLVCSD DLS+RM+FHTTD
Sbjct: 122 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 181

Query: 185 VMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVV 244
           VMEVT+   +YEVVFLAALVGLEEEEKGRVLRHLGKHMA GAYLMLRSAHGARAFLYPVV
Sbjct: 182 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 241

Query: 245 DICNVEASGFEILSVFHPTDEVINSVVIARK--KLVFDRNSIEDEDDDEDDEKQGILVNT 304
           D+ +VEASGFEILSVFHPTDEVINSVVIARK   LV D ++    D+D DDEK+ +LVNT
Sbjct: 242 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNY--NDNDTDDEKRAVLVNT 301

Query: 305 SSIVLPEKCSGFNGFSPMIEE 324
             I+L EKCSGF  F PMIEE
Sbjct: 302 -PIILAEKCSGFKDFGPMIEE 318

[Close]

BLAST of CmUC02G040540.1 vs. NCBI nr
Match: XP_023545298.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 496.9 bits (1278), Expect = 1.3e-136
Identity = 261/319 (81.82%), Postives = 284/319 (89.03%), Query Frame = 0


Query: 5   EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAH 64
           E  LL++VCALY+QISSLASL+PSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMRAH
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 61

Query: 65  LIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPA 124
           LIRLCA AEALLE+HFSSILA SF+NPI NLSIFPYYSNYLKLSLLEFDIL SH RRVP+
Sbjct: 62  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 121

Query: 125 TVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTD 184
           +VAF+GSGPLPLSSIV+AS HLKGTIFHNFDIDPAANTMA QLVCSD DLS+RM+FHT D
Sbjct: 122 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPAANTMAGQLVCSDSDLSKRMVFHTAD 181

Query: 185 VMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVV 244
           VMEVT+   +YEVVFLAALVGLEEEEKGRVLRHLGKHMA GAYLMLRSAHGARAFLYPVV
Sbjct: 182 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 241

Query: 245 DICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDEDDDEDDEKQGILVNTSS 304
           D+ +VEASGFEILSVFHPTDEVINSVVIARK      ++    D+D DDEK+ +LVN + 
Sbjct: 242 DVRDVEASGFEILSVFHPTDEVINSVVIARKVTDLVPDNDNYNDNDTDDEKRAVLVN-AP 301

Query: 305 IVLPEKCSGFNGFSPMIEE 324
           I+L EKCSGF  F PMIEE
Sbjct: 302 IILAEKCSGFKDFGPMIEE 318

[Close]

BLAST of CmUC02G040540.1 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.5e-95
Identity = 184/326 (56.44%), Postives = 246/326 (75.46%), Query Frame = 0


Query: 4   EEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRA 63
           ++  L+ K+C LY +IS L +L+P ++VDTLF QLV TC PP P +D+  +S+ +Q MR+
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRS 64

Query: 64  HLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRR-V 123
           +LI++C +AE  LE HFSSIL S  DNP+ +L++FPYY+NYLKLS LEFD+L  +    V
Sbjct: 65  NLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV 124

Query: 124 PATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHT 183
           P TVAF+GSGPLPL+S+VLAS HLK +IFHNFDIDP+AN +A++LV SDPDLSQRM FHT
Sbjct: 125 PRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHT 184

Query: 184 TDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYP 243
            D+M+VT+ LK ++VVFLAALVG++++EK +V+ HL KHM+ GA LMLRSAHG RAFLYP
Sbjct: 185 VDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYP 244

Query: 244 VVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDEDDDEDDEKQGILVNT 303
           +V+ C++E  GFE+LSV+HPTDEVINS+VI+R KL  D N +     D  D+   +  N 
Sbjct: 245 IVEPCDLE--GFEVLSVYHPTDEVINSIVISR-KLGEDANGVV---HDHIDQASDLACNC 304

Query: 304 SSI--VLPEKCSGFNGFSPMIEEQLS 327
           S I  ++ +K S    F+   EEQL+
Sbjct: 305 SKIHVIMNKKKSIIEEFAGANEEQLT 324

[Close]

BLAST of CmUC02G040540.1 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 6.7e-91
Identity = 168/276 (60.87%), Postives = 218/276 (78.99%), Query Frame = 0


Query: 1   MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQA 60
           M  ++  L+Q +C LY +IS L SL+PS++V+ LF QLV TC PP P +D+  +   +Q 
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQE 60

Query: 61  MRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYR 120
           +R +LI++C  AE  LE HFSSIL S  DNP+ +L+IFPYY+NYLKL  LEFD+L  +  
Sbjct: 61  IRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLN 120

Query: 121 R-VPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMI 180
             VP +VAF+GSGPLPL+SIVLAS HLK TIFHNFDIDP+AN++AS LV SDPD+SQRM 
Sbjct: 121 GFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMF 180

Query: 181 FHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAF 240
           FHT D+M+VT+ LK ++VVFLAALVG+ +EEK +V+ HL KHMA GA LMLRSAHG RAF
Sbjct: 181 FHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAF 240

Query: 241 LYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 276
           LYP+V+ C+++  GFE+LS++HPTD+VINSVVI++K
Sbjct: 241 LYPIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of CmUC02G040540.1 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 4.8e-89
Identity = 176/315 (55.87%), Postives = 230/315 (73.02%), Query Frame = 0


Query: 8   LLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAHLIR 67
           +++KVC LY QIS L +L PSK+V+ LFT LV TC PP P +D++ L Q +Q +R+HLI+
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP-IDVSKLCQKIQEIRSHLIK 68

Query: 68  LCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPATVA 127
           LC +AE LLE HFS IL SS++NP+ +L IFPY+ NY+KLSLLE++IL  +   +P  +A
Sbjct: 69  LCGQAEGLLESHFSKIL-SSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIA 128

Query: 128 FLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTDVME 187
           F+GSGPLPL+S+VLA+ HLK T FHN+DID  AN MAS LV +DPD+S RM FHT DVM+
Sbjct: 129 FIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMD 188

Query: 188 VTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVVDIC 247
           VT  LKDY+VVFLAALVG+++E+K +V+ HL K+M+ GA LMLRSAHGARAFLYPV+D  
Sbjct: 189 VTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPR 248

Query: 248 NVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDEDDDEDDEKQGILVNTSSIVL 307
           ++   GFE+LSV+HPTDEVINSV+IARK  V                   +L    + VL
Sbjct: 249 DLR--GFEVLSVYHPTDEVINSVIIARKLPV---------------PSVPLLDGLGAYVL 304

Query: 308 PEK--CSGFNGFSPM 321
           P K  C+  + F+P+
Sbjct: 309 PSKCACAEIHAFNPL 304

[Close]

BLAST of CmUC02G040540.1 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 4.1e-88
Identity = 164/276 (59.42%), Postives = 211/276 (76.45%), Query Frame = 0


Query: 1   MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASL-SQPLQ 60
           M  E   +++++  LYNQIS+L SL+PSKNVDTLF QLV TC P    +D+  +  + ++
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVK 60

Query: 61  AMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHY 120
            MR+HLI+LC +AE  LE HFS+IL S  DNP+++L IFPYY+NYLKL  LEFD+L  H 
Sbjct: 61  DMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHT 120

Query: 121 RRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMI 180
             VP  VAF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  D DLS+RMI
Sbjct: 121 THVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMI 180

Query: 181 FHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAF 240
           FHTTDV+   +GL  Y+VVFLAALVG+++E K + + HL KHMA GA +MLRSAHG RAF
Sbjct: 181 FHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAF 240

Query: 241 LYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 276
           LYP+VD C+++  GFE+L+++HP+D+V+NSVVIARK
Sbjct: 241 LYPIVDSCDLK--GFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of CmUC02G040540.1 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 3.8e-86
Identity = 161/270 (59.63%), Postives = 206/270 (76.30%), Query Frame = 0


Query: 8   LLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAHLIR 67
           +++++  LY+QIS L SL+PSKNVDTLF QLV TC P    +D+ ++ + ++ MRA+LI+
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 68  LCAKAEALLELHFSSILAS--SFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPAT 127
           LC +AE  LE HFS+IL S     NP+ +L IFPYYSNYLKL  LEFD+L  H   VP  
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 128 VAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTDV 187
           +AF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  DPDLS+RMIFHTTDV
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 188 MEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVVD 247
           +  T+GL  Y+VVFLAALVG+++E K + + HL KHMA GA LMLRSAH  RAFLYP+VD
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 248 ICNVEASGFEILSVFHPTDEVINSVVIARK 276
             + +  GF++L+++HPTD+V+NSVVIARK
Sbjct: 248 --SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of CmUC02G040540.1 vs. ExPASy TrEMBL
Match: A0A5D3BBH0 (Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001110 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.6e-154
Identity = 290/330 (87.88%), Postives = 311/330 (94.24%), Query Frame = 0


Query: 2   EFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCS---PPAP-GLDIASLSQP 61
           E +EA LLQKVCALYNQISSL+SL+P KNVDTLFTQLVLTCS   P AP G D+ASLSQP
Sbjct: 9   EQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQP 68

Query: 62  LQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRS 121
           LQAMRAHLI+LCAKAEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSLLEFDILRS
Sbjct: 69  LQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRS 128

Query: 122 HYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQR 181
           H RR+P  VAF+GSGPLPLSSIVLASIHLKGTIFHNFDIDP ANTMASQLVCSDPDLSQR
Sbjct: 129 HSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQR 188

Query: 182 MIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGAR 241
           MIFHT DVMEVTKGLKDYEVVFLAALVGLEEEEKGRVL+HLGKHMAAG+YLMLRSAHGAR
Sbjct: 189 MIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGAR 248

Query: 242 AFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDED-DDEDDEKQ 301
           AFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK+VFD++S+EDED D+E++EK 
Sbjct: 249 AFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDEDEEEEEKP 308

Query: 302 GILVNTSSIVLPEKCSGFNGFSPMIEEQLS 327
           GILV+TSSIVL EKCSGFNGF+P+IEEQLS
Sbjct: 309 GILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338

[Close]

BLAST of CmUC02G040540.1 vs. ExPASy TrEMBL
Match: A0A0A0LGI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.4e-152
Identity = 289/332 (87.05%), Postives = 307/332 (92.47%), Query Frame = 0


Query: 1   MEFE---EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCS---PPAPGLDIASL 60
           MEFE   EA LL+KVCALYNQISSL+SL+P KNVDTLFTQLVLTCS   PP  G DIASL
Sbjct: 1   MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL 60

Query: 61  SQPLQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDI 120
           SQPL+AMRAHLI+LCA+AEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSLLEFDI
Sbjct: 61  SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI 120

Query: 121 LRSHYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDL 180
           LRSH RR+P  VAF+GSGPLPLSSIVLASIHLKGTIFHNFDIDP ANTMASQLVCSDPDL
Sbjct: 121 LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL 180

Query: 181 SQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAH 240
           SQRMIFHT DVMEVTKGLKDYEVVFLAALVGL EEEKGRVL+HLGKHMAAG+YLMLRSAH
Sbjct: 181 SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH 240

Query: 241 GARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDEDDDEDDE 300
           GARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK++FD++S EDED   +DE
Sbjct: 241 GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDED---EDE 300

Query: 301 KQGILVNTSSIVLPEKCSGFNGFSPMIEEQLS 327
           KQGILVNTSSIVL EKCSGFNGF+PMIEE LS
Sbjct: 301 KQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

[Close]

BLAST of CmUC02G040540.1 vs. ExPASy TrEMBL
Match: A0A6J1G3L4 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.9e-137
Identity = 264/321 (82.24%), Postives = 287/321 (89.41%), Query Frame = 0


Query: 5   EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAH 64
           E  LL++VCALY+QISSLASL+PSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMRAH
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 61

Query: 65  LIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPA 124
           LIRLCA AEALLE+HFSSILA SF+NPI NLSIFPYYSNYLKLSLLEFDIL SH RRVP+
Sbjct: 62  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 121

Query: 125 TVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTD 184
           +VAF+GSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA QLVCSD DLS+RM+FHTTD
Sbjct: 122 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 181

Query: 185 VMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVV 244
           VMEVT+   +YEVVFLAALVGLEEEEKGRVLRHLGKHMA GAYLMLRSAHGARAFLYPVV
Sbjct: 182 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 241

Query: 245 DICNVEASGFEILSVFHPTDEVINSVVIARK--KLVFDRNSIEDEDDDEDDEKQGILVNT 304
           D+ +VEASGFEILSVFHPTDEVINSVVIARK   LV D ++    D+D DDEK+ +LVNT
Sbjct: 242 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNY--NDNDTDDEKRAVLVNT 301

Query: 305 SSIVLPEKCSGFNGFSPMIEE 324
             I+L EKCSGF  F PMIEE
Sbjct: 302 -PIILAEKCSGFKDFGPMIEE 318

[Close]

BLAST of CmUC02G040540.1 vs. ExPASy TrEMBL
Match: A0A6J1KIA7 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111493502 PE=3 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 5.5e-136
Identity = 260/321 (81.00%), Postives = 287/321 (89.41%), Query Frame = 0


Query: 5   EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAH 64
           E  LL++VCALY+QISSLASL+PSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMRAH
Sbjct: 32  EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 91

Query: 65  LIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPA 124
           LIRLCA AEALLE+HFSSILA SF+NPI NLSIFPYYSNYLKLSLLEFDIL SH RRVP+
Sbjct: 92  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILISHCRRVPS 151

Query: 125 TVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTD 184
           ++AF+GSGPLPLSSIV+AS HLKGTIFHNFDIDPAAN+MA +L+ SD DLS+RM+FHT D
Sbjct: 152 SIAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPAANSMARRLIRSDSDLSKRMVFHTAD 211

Query: 185 VMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVV 244
           VMEVTK   +YEVVFLAALVGLEEEEKGRVLRHLGKHMA GAYLMLRSAHGARAFLYPVV
Sbjct: 212 VMEVTKAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 271

Query: 245 DICNVEASGFEILSVFHPTDEVINSVVIARK--KLVFDRNSIEDEDDDEDDEKQGILVNT 304
           D+ ++EASGFEILSVFHPTDEVINSVVIARK   LV D N+    D++ DDEK+G+LVNT
Sbjct: 272 DVSDIEASGFEILSVFHPTDEVINSVVIARKVSDLVLDNNNY--NDNNTDDEKRGVLVNT 331

Query: 305 SSIVLPEKCSGFNGFSPMIEE 324
             I+L EKCSGF  F PMI+E
Sbjct: 332 -PIILAEKCSGFKDFGPMIDE 348

[Close]

BLAST of CmUC02G040540.1 vs. ExPASy TrEMBL
Match: A0A1S3BXI8 (probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.1e-133
Identity = 255/283 (90.11%), Postives = 267/283 (94.35%), Query Frame = 0


Query: 2   EFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCS---PPAP-GLDIASLSQP 61
           E +EA LLQKVCALYNQISSL+SL+P KNVDTLFTQLVLTCS   P AP G D+ASLSQP
Sbjct: 9   EQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQP 68

Query: 62  LQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRS 121
           LQAMRAHLI+LCAKAEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSLLEFDILRS
Sbjct: 69  LQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRS 128

Query: 122 HYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQR 181
           H RR+P  VAF+GSGPLPLSSIVLASIHLKGTIFHNFDIDP ANTMASQLVCSDPDLSQR
Sbjct: 129 HSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQR 188

Query: 182 MIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGAR 241
           MIFHT DVMEVTKGLKDYEVVFLAALVGLEEEEKGRVL+HLGKHMAAG+YLMLRSAHGAR
Sbjct: 189 MIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGAR 248

Query: 242 AFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFD 281
           AFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK+VFD
Sbjct: 249 AFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291

[Close]

BLAST of CmUC02G040540.1 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 349.7 bits (896), Expect = 2.5e-96
Identity = 184/326 (56.44%), Postives = 246/326 (75.46%), Query Frame = 0


Query: 4   EEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRA 63
           ++  L+ K+C LY +IS L +L+P ++VDTLF QLV TC PP P +D+  +S+ +Q MR+
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRS 64

Query: 64  HLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRR-V 123
           +LI++C +AE  LE HFSSIL S  DNP+ +L++FPYY+NYLKLS LEFD+L  +    V
Sbjct: 65  NLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV 124

Query: 124 PATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHT 183
           P TVAF+GSGPLPL+S+VLAS HLK +IFHNFDIDP+AN +A++LV SDPDLSQRM FHT
Sbjct: 125 PRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHT 184

Query: 184 TDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYP 243
            D+M+VT+ LK ++VVFLAALVG++++EK +V+ HL KHM+ GA LMLRSAHG RAFLYP
Sbjct: 185 VDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYP 244

Query: 244 VVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDEDDDEDDEKQGILVNT 303
           +V+ C++E  GFE+LSV+HPTDEVINS+VI+R KL  D N +     D  D+   +  N 
Sbjct: 245 IVEPCDLE--GFEVLSVYHPTDEVINSIVISR-KLGEDANGVV---HDHIDQASDLACNC 304

Query: 304 SSI--VLPEKCSGFNGFSPMIEEQLS 327
           S I  ++ +K S    F+   EEQL+
Sbjct: 305 SKIHVIMNKKKSIIEEFAGANEEQLT 324

[Close]

BLAST of CmUC02G040540.1 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 335.5 bits (859), Expect = 4.8e-92
Identity = 168/276 (60.87%), Postives = 218/276 (78.99%), Query Frame = 0


Query: 1   MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQA 60
           M  ++  L+Q +C LY +IS L SL+PS++V+ LF QLV TC PP P +D+  +   +Q 
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQE 60

Query: 61  MRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYR 120
           +R +LI++C  AE  LE HFSSIL S  DNP+ +L+IFPYY+NYLKL  LEFD+L  +  
Sbjct: 61  IRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLN 120

Query: 121 R-VPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMI 180
             VP +VAF+GSGPLPL+SIVLAS HLK TIFHNFDIDP+AN++AS LV SDPD+SQRM 
Sbjct: 121 GFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMF 180

Query: 181 FHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAF 240
           FHT D+M+VT+ LK ++VVFLAALVG+ +EEK +V+ HL KHMA GA LMLRSAHG RAF
Sbjct: 181 FHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAF 240

Query: 241 LYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 276
           LYP+V+ C+++  GFE+LS++HPTD+VINSVVI++K
Sbjct: 241 LYPIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of CmUC02G040540.1 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 326.2 bits (835), Expect = 2.9e-89
Identity = 164/276 (59.42%), Postives = 211/276 (76.45%), Query Frame = 0


Query: 1   MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASL-SQPLQ 60
           M  E   +++++  LYNQIS+L SL+PSKNVDTLF QLV TC P    +D+  +  + ++
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVK 60

Query: 61  AMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHY 120
            MR+HLI+LC +AE  LE HFS+IL S  DNP+++L IFPYY+NYLKL  LEFD+L  H 
Sbjct: 61  DMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHT 120

Query: 121 RRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMI 180
             VP  VAF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  D DLS+RMI
Sbjct: 121 THVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMI 180

Query: 181 FHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAF 240
           FHTTDV+   +GL  Y+VVFLAALVG+++E K + + HL KHMA GA +MLRSAHG RAF
Sbjct: 181 FHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAF 240

Query: 241 LYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 276
           LYP+VD C+++  GFE+L+++HP+D+V+NSVVIARK
Sbjct: 241 LYPIVDSCDLK--GFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of CmUC02G040540.1 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 319.7 bits (818), Expect = 2.7e-87
Identity = 161/270 (59.63%), Postives = 206/270 (76.30%), Query Frame = 0


Query: 8   LLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAHLIR 67
           +++++  LY+QIS L SL+PSKNVDTLF QLV TC P    +D+ ++ + ++ MRA+LI+
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 68  LCAKAEALLELHFSSILAS--SFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPAT 127
           LC +AE  LE HFS+IL S     NP+ +L IFPYYSNYLKL  LEFD+L  H   VP  
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 128 VAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTDV 187
           +AF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  DPDLS+RMIFHTTDV
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 188 MEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVVD 247
           +  T+GL  Y+VVFLAALVG+++E K + + HL KHMA GA LMLRSAH  RAFLYP+VD
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 248 ICNVEASGFEILSVFHPTDEVINSVVIARK 276
             + +  GF++L+++HPTD+V+NSVVIARK
Sbjct: 248 --SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of CmUC02G040540.1 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 57.0 bits (136), Expect = 3.3e-08
Identity = 30/56 (53.57%), Postives = 41/56 (73.21%), Query Frame = 0


Query: 222 MAAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHP--TDEVINSVVIARK 276
           M  GA LMLRSA G R+FLY  VD C+++  GFE+L ++HP  +D  +NSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLK--GFEVLEIYHPSMSDGFVNSVMVARK 54

The following BLAST results are available for this feature:

BLAST of CmUC02G040540.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890564.1	2.5e-159	90.55	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
KAA0048069.1	1.6e-153	87.88	putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa] >TYJ9646...	[more]
XP_004144750.1	1.1e-151	87.05	probable nicotianamine synthase 4 [Cucumis sativus] >KGN61000.1 hypothetical pro...	[more]
XP_022946411.1	6.0e-137	82.24	nicotianamine synthase-like [Cucurbita moschata]	[more]
XP_023545298.1	1.3e-136	81.82	nicotianamine synthase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmUC02G040540.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7X5	3.5e-95	56.44	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	6.7e-91	60.87	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9XGI7	4.8e-89	55.87	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9FKT9	4.1e-88	59.42	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]
Q9FF79	3.8e-86	59.63	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]

BLAST of CmUC02G040540.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BBH0	7.6e-154	87.88	Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A0A0LGI4	5.4e-152	87.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1	[more]
A0A6J1G3L4	2.9e-137	82.24	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 S...	[more]
A0A6J1KIA7	5.5e-136	81.00	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111493502 PE=3 SV=...	[more]
A0A1S3BXI8	1.1e-133	90.11	probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]

BLAST of CmUC02G040540.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	2.5e-96	56.44	nicotianamine synthase 4	[more]
AT1G09240.1	4.8e-92	60.87	nicotianamine synthase 3	[more]
AT5G56080.1	2.9e-89	59.42	nicotianamine synthase 2	[more]
AT5G04950.1	2.7e-87	59.63	nicotianamine synthase 1	[more]
AT4G26483.1	3.3e-08	53.57	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 5..277 e-value: 6.4E-117 score: 389.8
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 4..277
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..280 score: 55.351879
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 9..276 e-value: 4.5E-60 score: 205.0

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmUC02G040540	CmUC02G040540	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmUC02G040540.1-exon	CmUC02G040540.1-exon-CmU531Chr02:28354566..28355104	exon
CmUC02G040540.1-exon	CmUC02G040540.1-exon-CmU531Chr02:28355781..28356222	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmUC02G040540.1-cds	CmUC02G040540.1-cds-CmU531Chr02:28354566..28355104	CDS
CmUC02G040540.1-cds	CmUC02G040540.1-cds-CmU531Chr02:28355781..28356222	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmUC02G040540.1	CmUC02G040540.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity