ClCG04G000960.1 (mRNA) Watermelon (Charleston Gray) v2.5

Overview
NameClCG04G000960.1
TypemRNA
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionRING-type E3 ubiquitin transferase
LocationCG_Chr04: 3138412 .. 3143471 (+)
Sequence length2633
RNA-Seq ExpressionClCG04G000960.1
SyntenyClCG04G000960.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTTCTTTATTTTAATCCCTTTTTCAATACCGTTTCTGTTTTTGTTTTCAACGTTCGTATGGATTTTTCCACTTTTGAGACCCCCAGAATTTATTATTTGAATTGCTGATCTGTTTCTGTAATTGGGCTCCGTATTCTCATCTGGGGATATGGGATTTTTGTTTTTTTTGTGAGATTTTCTTCTGTTATTGTTATTGGTTTTTGTGGGGTTCTGTTTTTACTACTGGAGACTTGTTAATGAGGGTCTTGTTTTGTTTCGTTCCTTAAGTTTCGTGCAGATTTTGTTTAGTTGTTGAATATTGATTTTCGAATTGGAGAAATGGTTTCTCAATTTGTAGTTTGTAAAAGGGGAGGGACAGGTTTTTGCATTTGTGCTGCCTGGTCTGGGTTCTTTTCAATTTCTAGCCATTTTTTGAGCACTTTTCGGTGGATGAAGTTGGATTTAATCACACGGTTTTTGGAATTTGACTGAAATACGGTTGTTTTGATTGGCTTCTGAGCCTCTAAAGTGATGCCTTTTTGTTTATTATGTTGGTCCTGATGGATTCAAATGAGCCAATAATTTCATTAGGTGATTTATCTCCACAATTTACAGAGCATTCGATAGAAATGCAACTTTTTGTTAGACTATGTTATGCTAGTTGTCCTTTTTCTATAGAATATAATGCTGTCTTATGTCATAATATGGAGCAAGCTCTTTGTAATATTTGGGGTTTTACTATTGCTTTGGGTTTGGAAGGAATCTAGATTTCTTACTATTTATCTCATCAATGTTTACAAATTAAGAGTTCATCAAACTTAATAAGTTTCAAGTTGATCTTTCAGTATTTTGAAGCTCTTTTCACAATGAAAGCCTGGGTGTGGATGTTTATGTGCATGTGCCTTGGCACATTCCAACGTTAAACCAGGATGTTAAACACATAGATCTGTTAACCTAGGCTTCTTAAGTTTGGGGTTGGAGGTCTGAGTTTTTATGCTTGGACTATAAAATAGAATGGGTTGTTAAATCTTGAGGTTTGTGTAATTTGTGTTTGGTTTAGGATTTATAATTTATAAAGTTTATATGTAGTATTCGTATAAAAGGTGGGTTTTTGTCCTTGAGTTTGTGTACTTTGTAGGATTAGCAAACTTGTAGCTTGGAACAAGAAATTCCAACAACCAAACATAGGCCTAACATCTTTTTAACTCCAAATTATATAAATCTAACCATGAGCTAGTCACCACATATTGAGGAAAGAATCTAGCAAATGGTCAGCAAGCATTATGAGATAGAGTTGGACTTCAGGTTATGTAAGTGTCCTAACCTATGGCATATAGTTACCATTTTCCAGTTCAGATGTTAAGAAAATGAGGGTGTTGCTAAGAGTTGTATGTTATTAAGCATGTTGATCATATTTAGTTTTCATTATCAACTTTCATTCAAATTATATCAACAAATCTACAATCAACACAAAATGTTTCGTCTAATATGTTTATTGACATGCCCTTTTCTTTTTTGTCTGGTTTAACAGGATTGAATTCATGGATGGGTGATTGTTAGCATATGGTGAAGAGTTTCCATTAATACCTTAAAGAACTCTATTAAAATTTAAATAAGCCATGGCTCCAGGAAGCAGTACCTGCAAAGAAGTTGTTGATTCTCATCCTTCAGTTTCAGGTCATGACATAGCAATGTCTAAATCAGAGAATATTTCTACATCCTCAAAAGGCCCACTTGGTTTGAGTGGGAAAATTGGAGTTTATTCAACCAATGGTGTGCAAGAATTGCTTGAGTGCCCTGTCTGCACAAATCTGATGTACCCTCCAATTTACCAGGTATGTACTTTATACCCTGTTTTTTCTATTGTACATTTCATTATAATTTAAGCTATGCTTGGGATTGTTGGGAGTGTTTTGGCATGGAAAAAAGGGTACACTTCTCGCTCAAATTCATTATGTTTGGGAGTAACATAAAAATGATTTCAGAAAAAGTCAAAAGCACCTTAAAAGGCTGTTTGATAAAGCTCTTTCTAATGCGTGTAAAGTATTTTCCTAACTGCACCTGTTGCCAATTGCCTGCGTAAGCATGGAGAAGTGCTTTTGCAAAATTACCTTCATTTGTAAAGCTTCTTCCATATTGTGCTTTAGATTCGTTGCAATGATTGGGTCCAATGAACGAAAGCTGCTGTTAGTTTCTTTAAGGTTGAATGGTGTGTGTTGAATATTATTAGTTGCTTATCTCATTCTTTTGCAAAATTGTATAGAATGTTCCCTTAGAAATATGAGGTCCATGCATACTGGAATGGGTGTTCTTCAGCTTCCTTTGAGCTTCTTATTTGAGCAATTGATTGATTCTCTCTCTCCTTCCCTTCCCCCCCAAATGGAGAGTAGTAAAGTTTTTTCTTGAACTTAGTTTTTCTTTAGAATCAAAGTATTCATAGGTTATTTTATATATTTTATATTTATTAGGATTTTTCAATAAATGGCTAAAAGGTCATGGTTTCAATCCATTGTGACCACTTACATAGGATTTAATATCCTACGAGTTTCCTTGACACTCAAATATTGTAGGGTCAGGTGGTTGTACCGTAAGATTAGTCGAGGTGCATGTAAGCTAGCCCAGACACTCGCGGATATCGAAAAGAAAAAAAAAAATAGAAGTCTCTCTGACCCTCTTGAATTTTACATAACTTAGTCCAAAGTCTCTTGGTGATTAACATAGTTCCTTTTCTTTTTTCTTTTTTTTTTTTTTGATTCTGCTTTTGCTTTTCTGTTTTTAGAAATCATTCTTTTTTACTTGCCATTTTCGTTTTACAGTGTCCATTCTGAAATACTGACCAAATTTCAGAAACAGTTATTGATAACTTTTTCTTTTTAGTTTTTAAAATTATCTAATATTTCTAAAACGTTTTGATAGTATGTTGCAAATCAAAGAAATGTGCTAGTAAATGAACACTTTTGAAAACAGAAACTTTTCTAGCTTCTTGTTTTTTTTCAATTTCTGTTTCTTAATCTTTTTGAATTGCCATATAAATTGAAGAATCCGAACTTCTGTACATAACAACTGTGACATATCTTATTTTGTTAAGGAAATAACTGTGGCTTTCTGCAGTTTTAAAGGCATATATTAAGAATTTGTATGGCCTAATAGCTTAATTCTTGATATAATCTTCATTGGAGGGTTGTTTTGGACTCTTTATTGTGGCCAATTCTGAAGTTCTTTTCTTAATATTAAATTTGTATTGAAGACTTGGAACATCTCTTTTTTCTTGAATCTTCATGACATGGAGGTGTGATATACTTTTCTGCTTCTGCTTTGTTTCGGACTTTCTTGACTGGCTTTTGAACTTTAGTGCCCAAATGGCCACACATTGTGCTCAAACTGCAAGATCAGAGTTAGCAACTGTTGCCCGACCTGTCGTCATGAACTTGGAAACATTAGGTGCTTGGCACTGGAAAAGGTAGCTGAGTCGCTGGAAATCCCCTGCAAATACCAGAATTTGGGCTGTCAAGATATTTTTCCCTACTACAGTAAGCTTAAGCATGAGCAACACTGTCGATACCGTCCTTACAACTGCCCATATGCTGGATCAGAGTGTTCGGTCACGGGTGACATCCCGTCTCTTGTTGCACATCTCAAAGATGATCACAAGGTTGACATGCATGATGGGTGTACTTTCAATCATCGCTATGTGAAATCAAATCCACACGAAGTTGAAAATGCCACATGGATGCTAACGGTAATTGCTTGTTATATACCATTATATATTTTTCACAATGATAATTTCATGTAACATTAATGTCACACCCTACCCCAGATTACCCTACTGAATCTGGAACGAGATGTGACGACAATAGATTCCGAGTGTGTATTGGAATCTATTGTCAAAACTTTTCTTATCTTTAAACTTTACTTCTTTTATACTTCCATATATTTAGCATACACATTGTGTCATATGATATTTTTAATTTACACTTAAATGTCTAGCTAAATATACATACAAAAGGTGATATGTGACCGCGAAACTTGTAGCAGAAACTGGACAACTGACCTTAATTGAAACCCAAATACTAAGACACATAAAAATGGGTCTTACAATTAACTTTCACCCGTTGGCTTAATAGTCTACCAGGGCCTTTAGGGCTTTAGAAGGAATGAATTCAGGCCATGGTTTATAAGTTTTCTTAAAAATCAAATGTAGTACAGCCCCGTGGAATTGTGAGAATAGTTAAAGAGCTCAAGCACTCATGACTATTAAAAAAAATCAATTAACTATATATATATTTTGATAAGTTGATGATTGCTACATTTGTACTGACGAACTTTTAGGAGGGTTTTATTTTAAAGAGGTTGCACTTCTAATTTTCATATATAACTTTTAGGTAAAGATCTGATAAATCTAGTTTTCTTGTATCTTTATGTTGGCAGGTTTTCAACTGCTTTGGAAAGCAGTTCTGCTTGCATTTTGAGGCATTCCAGCTAGGAATGGCACCTGTTTACATGGCGTTTTTACGATTCATGGGTGACGATAACGAAGCAAAGAAGTTCAGCTACAGTTTGGAAGTGGGTGGCAATGGGCGTAAACTGATATGGCAAGGAATCCCGAGGGGCATCCGCGACAGTCACCGAAAAGTTCGTGACAGTCAGGACGGTCTCATTATTCAAAGAAACCTGGCACTGTATTTTTCGGGAGGAGAAAGGCAAGAGCTGAAGCTGAGAATTACAGGTCGAATATGGAAAGAAGAATGAGATCGAGGGACAGCGCTCCATGGTGTTGAGGTGAATGTATTATTGTACAGTTGAGACAAGTTTGTTAGGCCGACACTCAACAGCAGGGGTTTGCTCCAAGTGCTGAAGAAACTTTGGAGAAACTTCCAAGCTAATATCAAAAATGTAATTTACCAAGTGAATCAAAAAAGCATTTTGCCGCCTCATATTTTATGTATTTAAAGCCAAGTTTCTCCTTGTTTTTCTTTTGTTGGTATCATTGATTAGCTCTGCATTGCAAATTGTTTGTTTTGCAGGTTTCCAATGGTTGTCTATAGAATGGAGTTTTGGGGGGTTGCAACCATGTGTG

mRNA sequence

TTTTTTTCTTTATTTTAATCCCTTTTTCAATACCGTTTCTGTTTTTGTTTTCAACGTTCGTATGGATTTTTCCACTTTTGAGACCCCCAGAATTTATTATTTGAATTGCTGATCTGTTTCTGTAATTGGGCTCCGTATTCTCATCTGGGGATATGGGATTTTTGTTTTTTTTGTGAGATTTTCTTCTGTTATTGTTATTGGTTTTTGTGGGGTTCTGTTTTTACTACTGGAGACTTGTTAATGAGGGTCTTGTTTTGTTTCGTTCCTTAAGTTTCGTGCAGATTTTGTTTAGTTGTTGAATATTGATTTTCGAATTGGAGAAATGGTTTCTCAATTTGTAGTTTGTAAAAGGGGAGGGACAGGTTTTTGCATTTGTGCTGCCTGGTCTGGGTTCTTTTCAATTTCTAGCCATTTTTTGAGCACTTTTCGGTGGATGAAGTTGGATTTAATCACACGGTTTTTGGAATTTGACTGAAATACGGTTGTTTTGATTGGCTTCTGAGCCTCTAAAGTGATGCCTTTTTGTTTATTATGTTGGTCCTGATGGATTCAAATGAGCCAATAATTTCATTAGGTGATTTATCTCCACAATTTACAGAGCATTCGATAGAAATGCAACTTTTTGTTAGACTATGTTATGCTAGTTGTCCTTTTTCTATAGAATATAATGCTGTCTTATGTCATAATATGGAGCAAGCTCTTTGTAATATTTGGGGTTTTACTATTGCTTTGGGTTTGGAAGGAATCTAGATTTCTTACTATTTATCTCATCAATGTTTACAAATTAAGAGTTCATCAAACTTAATAAGTTTCAAGTTGATCTTTCAGTATTTTGAAGCTCTTTTCACAATGAAAGCCTGGGTGTGGATGTTTATGTGCATGTGCCTTGGCACATTCCAACGTTAAACCAGGATGTTAAACACATAGATCTGTTAACCTAGGCTTCTTAAGTTTGGGGTTGGAGGTCTGAGTTTTTATGCTTGGACTATAAAATAGAATGGGTTGTTAAATCTTGAGGTTTGTGTAATTTGTGTTTGGTTTAGGATTTATAATTTATAAAGTTTATATGTAGTATTCGTATAAAAGGTGGGTTTTTGTCCTTGAGTTTGTGTACTTTGTAGGATTAGCAAACTTGTAGCTTGGAACAAGAAATTCCAACAACCAAACATAGGCCTAACATCTTTTTAACTCCAAATTATATAAATCTAACCATGAGCTAGTCACCACATATTGAGGAAAGAATCTAGCAAATGGTCAGCAAGCATTATGAGATAGAGTTGGACTTCAGGTTATGATTGAATTCATGGATGGGTGATTGTTAGCATATGGTGAAGAGTTTCCATTAATACCTTAAAGAACTCTATTAAAATTTAAATAAGCCATGGCTCCAGGAAGCAGTACCTGCAAAGAAGTTGTTGATTCTCATCCTTCAGTTTCAGGTCATGACATAGCAATGTCTAAATCAGAGAATATTTCTACATCCTCAAAAGGCCCACTTGGTTTGAGTGGGAAAATTGGAGTTTATTCAACCAATGGTGTGCAAGAATTGCTTGAGTGCCCTGTCTGCACAAATCTGATGTACCCTCCAATTTACCAGTGCCCAAATGGCCACACATTGTGCTCAAACTGCAAGATCAGAGTTAGCAACTGTTGCCCGACCTGTCGTCATGAACTTGGAAACATTAGGTGCTTGGCACTGGAAAAGGTAGCTGAGTCGCTGGAAATCCCCTGCAAATACCAGAATTTGGGCTGTCAAGATATTTTTCCCTACTACAGTAAGCTTAAGCATGAGCAACACTGTCGATACCGTCCTTACAACTGCCCATATGCTGGATCAGAGTGTTCGGTCACGGGTGACATCCCGTCTCTTGTTGCACATCTCAAAGATGATCACAAGGTTGACATGCATGATGGGTGTACTTTCAATCATCGCTATGTGAAATCAAATCCACACGAAGTTGAAAATGCCACATGGATGCTAACGGTTTTCAACTGCTTTGGAAAGCAGTTCTGCTTGCATTTTGAGGCATTCCAGCTAGGAATGGCACCTGTTTACATGGCGTTTTTACGATTCATGGGTGACGATAACGAAGCAAAGAAGTTCAGCTACAGTTTGGAAGTGGGTGGCAATGGGCGTAAACTGATATGGCAAGGAATCCCGAGGGGCATCCGCGACAGTCACCGAAAAGTTCGTGACAGTCAGGACGGTCTCATTATTCAAAGAAACCTGGCACTGTATTTTTCGGGAGGAGAAAGGCAAGAGCTGAAGCTGAGAATTACAGGTCGAATATGGAAAGAAGAATGAGATCGAGGGACAGCGCTCCATGGTGTTGAGGTGAATGTATTATTGTACAGTTGAGACAAGTTTGTTAGGCCGACACTCAACAGCAGGGGTTTGCTCCAAGTGCTGAAGAAACTTTGGAGAAACTTCCAAGCTAATATCAAAAATGTAATTTACCAAGTGAATCAAAAAAGCATTTTGCCGCCTCATATTTTATGTATTTAAAGCCAAGTTTCTCCTTGTTTTTCTTTTGTTGGTATCATTGATTAGCTCTGCATTGCAAATTGTTTGTTTTGCAGGTTTCCAATGGTTGTCTATAGAATGGAGTTTTGGGGGGTTGCAACCATGTGTG

Coding sequence (CDS)

ATGGCTCCAGGAAGCAGTACCTGCAAAGAAGTTGTTGATTCTCATCCTTCAGTTTCAGGTCATGACATAGCAATGTCTAAATCAGAGAATATTTCTACATCCTCAAAAGGCCCACTTGGTTTGAGTGGGAAAATTGGAGTTTATTCAACCAATGGTGTGCAAGAATTGCTTGAGTGCCCTGTCTGCACAAATCTGATGTACCCTCCAATTTACCAGTGCCCAAATGGCCACACATTGTGCTCAAACTGCAAGATCAGAGTTAGCAACTGTTGCCCGACCTGTCGTCATGAACTTGGAAACATTAGGTGCTTGGCACTGGAAAAGGTAGCTGAGTCGCTGGAAATCCCCTGCAAATACCAGAATTTGGGCTGTCAAGATATTTTTCCCTACTACAGTAAGCTTAAGCATGAGCAACACTGTCGATACCGTCCTTACAACTGCCCATATGCTGGATCAGAGTGTTCGGTCACGGGTGACATCCCGTCTCTTGTTGCACATCTCAAAGATGATCACAAGGTTGACATGCATGATGGGTGTACTTTCAATCATCGCTATGTGAAATCAAATCCACACGAAGTTGAAAATGCCACATGGATGCTAACGGTTTTCAACTGCTTTGGAAAGCAGTTCTGCTTGCATTTTGAGGCATTCCAGCTAGGAATGGCACCTGTTTACATGGCGTTTTTACGATTCATGGGTGACGATAACGAAGCAAAGAAGTTCAGCTACAGTTTGGAAGTGGGTGGCAATGGGCGTAAACTGATATGGCAAGGAATCCCGAGGGGCATCCGCGACAGTCACCGAAAAGTTCGTGACAGTCAGGACGGTCTCATTATTCAAAGAAACCTGGCACTGTATTTTTCGGGAGGAGAAAGGCAAGAGCTGAAGCTGAGAATTACAGGTCGAATATGGAAAGAAGAATGA

Protein sequence

MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQNLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIWKEE
Homology
BLAST of ClCG04G000960.1 vs. NCBI nr
Match: XP_038881778.1 (E3 ubiquitin-protein ligase SINAT2-like [Benincasa hispida] >XP_038881779.1 E3 ubiquitin-protein ligase SINAT2-like [Benincasa hispida])

HSP 1 Score: 645.6 bits (1664), Expect = 2.2e-181
Identity = 301/307 (98.05%), Postives = 304/307 (99.02%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPGSSTCKEVVDSHPSVSGHDIAMSK+EN STSSK  LGLSGKIGVYSTNGVQELLECP
Sbjct: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKTENNSTSSKAALGLSGKIGVYSTNGVQELLECP 60

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VCTNLMYPPIYQCP+GHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPC+YQ
Sbjct: 61  VCTNLMYPPIYQCPSGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 120

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT
Sbjct: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 301 GRIWKEE 307

BLAST of ClCG04G000960.1 vs. NCBI nr
Match: XP_022928730.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928731.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928732.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928733.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928734.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 634.0 bits (1634), Expect = 6.6e-178
Identity = 296/307 (96.42%), Postives = 301/307 (98.05%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPGSSTCKEVVDSHPSVSGHDIAMSKSEN STSSK  LG SGK+GVYSTNGVQELLECP
Sbjct: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPC+YQ
Sbjct: 63  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCR+RPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT
Sbjct: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLG APVYMAFLRFMG+DNEAKK
Sbjct: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT
Sbjct: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 302

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 303 GRIWKEE 309

BLAST of ClCG04G000960.1 vs. NCBI nr
Match: XP_022967856.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita maxima] >XP_023543585.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543586.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543587.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 632.5 bits (1630), Expect = 1.9e-177
Identity = 295/307 (96.09%), Postives = 301/307 (98.05%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPGSSTCKEVVDSHPSVSGHDIAMSKSEN STSSK  LG SGK+GVYSTNGVQELLECP
Sbjct: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPC+YQ
Sbjct: 63  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCR+RPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT
Sbjct: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLG APVYMAFLRFMG+DNEAKK
Sbjct: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELKLRIT
Sbjct: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRIT 302

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 303 GRIWKEE 309

BLAST of ClCG04G000960.1 vs. NCBI nr
Match: KAG6603259.1 (E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033559.1 E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 630.2 bits (1624), Expect = 9.5e-177
Identity = 294/307 (95.77%), Postives = 300/307 (97.72%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPGSSTCKEVVDSHPSVSGHDIAMSKSEN STSSK  LG SGK+GVYSTNGVQELLECP
Sbjct: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VC NLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPC+YQ
Sbjct: 63  VCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCR+RPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT
Sbjct: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLG APVYMAFLRFMG+DNEAKK
Sbjct: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELKLRIT
Sbjct: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRIT 302

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 303 GRIWKEE 309

BLAST of ClCG04G000960.1 vs. NCBI nr
Match: XP_023518392.1 (E3 ubiquitin-protein ligase SINAT2-like [Cucurbita pepo subsp. pepo] >XP_023518393.1 E3 ubiquitin-protein ligase SINAT2-like [Cucurbita pepo subsp. pepo] >KAG6595627.1 E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027600.1 E3 ubiquitin-protein ligase SINAT2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 624.4 bits (1609), Expect = 5.2e-175
Identity = 292/307 (95.11%), Postives = 299/307 (97.39%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPGSSTCK+ VDSHPSVSGHDIA+SKSEN  T+SK  LGL GK+GVYSTNGVQELLECP
Sbjct: 1   MAPGSSTCKK-VDSHPSVSGHDIAISKSENNPTTSKASLGLGGKLGVYSTNGVQELLECP 60

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VCTNLMYPPIYQCPNGHTLCSNCKIRVSN CPTCRHELGNIRCLALEKVAESLEIPC+YQ
Sbjct: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNSCPTCRHELGNIRCLALEKVAESLEIPCRYQ 120

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCR+RPYNCPYAGSECSVTGDI SLVAHLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIQSLVAHLKDDHKVDMHDGCT 180

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT
Sbjct: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 301 GRIWKEE 306

BLAST of ClCG04G000960.1 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 2.8e-155
Identity = 253/309 (81.88%), Postives = 277/309 (89.64%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSE--NISTSSKGPLGLSGKIGVYSTNGVQELLE 60
           MAPG S  KEV++S+ +   +++  +K E  N   +  G  G+ GK G++S NGV ELLE
Sbjct: 1   MAPGGSALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGI-GKYGIHSNNGVYELLE 60

Query: 61  CPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCK 120
           CPVCTNLMYPPI+QCPNGHTLCSNCK+RV N CPTCR+ELGNIRCLALEKVAESLE+PC+
Sbjct: 61  CPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCR 120

Query: 121 YQNLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180
           YQNLGC DIFPYYSKLKHEQHCR+RPY CPYAGSECSVTGDIP+LV HLKDDHKVDMHDG
Sbjct: 121 YQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDG 180

Query: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEA 240
           CTFNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLGMAPVYMAFLRFMGD+NEA
Sbjct: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEA 240

Query: 241 KKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLR 300
           KKFSYSLEVG +GRKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSGG+RQELKLR
Sbjct: 241 KKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLR 300

Query: 301 ITGRIWKEE 308
           +TGRIWKEE
Sbjct: 301 VTGRIWKEE 308

BLAST of ClCG04G000960.1 vs. ExPASy Swiss-Prot
Match: P93748 (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=SINAT1 PE=1 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 2.4e-151
Identity = 247/307 (80.46%), Postives = 274/307 (89.25%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPG S  KE ++S+ +   +++ M+K E  S  +K   G  GK   +S+NGV ELLECP
Sbjct: 1   MAPGGSALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKF--HSSNGVYELLECP 60

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VCTNLMYPPI+QCPNGHTLCS+CK+RV N CPTCR+ELGNIRCLALEKVAESLE+PC+YQ
Sbjct: 61  VCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQ 120

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCR+R Y+CPYAGSECSVTGDIP+LV HLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCT 180

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLGMAPVYMAFLRFMGD+NEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKK 240

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVG + RKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSG +++ELKLR+T
Sbjct: 241 FSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVT 300

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 301 GRIWKEE 305

BLAST of ClCG04G000960.1 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 3.6e-134
Identity = 216/270 (80.00%), Postives = 239/270 (88.52%), Query Frame = 0

Query: 38  PLGLSGKIGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHE 97
           P+  S K  V  ++ V+ELLECPVC + MYPPI+QC NGHTLCS CK RV N CPTCRHE
Sbjct: 32  PVPHSPKPNVVVSSSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHE 91

Query: 98  LGNIRCLALEKVAESLEIPCKYQNLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVT 157
           LGNIRCLALEKVA SLE+PCKYQN GC  I+PYY KLKHE  C+YRPY+CPYAGSEC+V 
Sbjct: 92  LGNIRCLALEKVAASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVA 151

Query: 158 GDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAF 217
           GDIP LV HLKDDHKVDMH+GCTFNHRYVKSNPHEVENATWMLTVF+CFG+ FCLHFEAF
Sbjct: 152 GDIPYLVNHLKDDHKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAF 211

Query: 218 QLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGL 277
           QLGMAPVY+AFLRFMGDD EAK +SYSLEVGG GRK+IWQG+PR IRDSHRKVRDS DGL
Sbjct: 212 QLGMAPVYIAFLRFMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGL 271

Query: 278 IIQRNLALYFSGGERQELKLRITGRIWKEE 308
           IIQRN+AL+FSGGER+ELKLR+TGRIWKE+
Sbjct: 272 IIQRNMALFFSGGERKELKLRVTGRIWKEQ 301

BLAST of ClCG04G000960.1 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 5.1e-133
Identity = 218/294 (74.15%), Postives = 249/294 (84.69%), Query Frame = 0

Query: 14  SHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECPVCTNLMYPPIYQC 73
           S+ SVS H    + + N++ ++ G L         +T  V ELLECPVCTN MYPPI+QC
Sbjct: 26  SYASVSKHHHTNNNTTNVNAAASGLLP--------TTTSVHELLECPVCTNSMYPPIHQC 85

Query: 74  PNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQNLGCQDIFPYYSK 133
            NGHTLCS CK RV N CPTCR ELG+IRCLALEKVAESLE+PCK+ +LGC +IFPYYSK
Sbjct: 86  HNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFPYYSK 145

Query: 134 LKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEV 193
           LKHE  C +RPY+CPYAGSECSVTGDIP LVAHL+DDHKVDMH GCTFNHRYVKSNP EV
Sbjct: 146 LKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREV 205

Query: 194 ENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRK 253
           ENATWMLTVF+CFG+ FCLHFEAFQLGMAPVYMAFLRFMGD+ EA+ ++YSLEVGG GRK
Sbjct: 206 ENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGRK 265

Query: 254 LIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIWKEE 308
           LIW+G PR +RDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TGRIWKE+
Sbjct: 266 LIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 311

BLAST of ClCG04G000960.1 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 461.5 bits (1186), Expect = 7.7e-129
Identity = 213/295 (72.20%), Postives = 241/295 (81.69%), Query Frame = 0

Query: 13  DSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECPVCTNLMYPPIYQ 72
           DS  SV   D    K +  ST S+G       + +     V ELLECPVCTN MYPPI+Q
Sbjct: 4   DSIDSVIDDDEIHQKHQFSSTKSQG----GATVVISPATSVYELLECPVCTNSMYPPIHQ 63

Query: 73  CPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQNLGCQDIFPYYS 132
           C NGHTLCS CK RV N CPTCR ELG+IRCLALEKVAESLE+PCKY NLGC  IFPYYS
Sbjct: 64  CHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYNLGCLGIFPYYS 123

Query: 133 KLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHE 192
           KLKHE  C +RPY+CPYAGSEC+  GDI  LVAHL+DDHKVDMH GCTFNHRYVKSNP E
Sbjct: 124 KLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHKVDMHTGCTFNHRYVKSNPRE 183

Query: 193 VENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGR 252
           VENATWMLTVF CFG+ FCLHFEAFQLGMAPVYMAFLRFMGD+++A+ ++YSLEVGG+GR
Sbjct: 184 VENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGR 243

Query: 253 KLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIWKEE 308
           K  W+G PR +RDSHRKVRDS DGLIIQRN+AL+FSGG+++ELKLR+TGRIWKE+
Sbjct: 244 KQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWKEQ 294

BLAST of ClCG04G000960.1 vs. ExPASy TrEMBL
Match: A0A6J1ESE8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111435553 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 3.2e-178
Identity = 296/307 (96.42%), Postives = 301/307 (98.05%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPGSSTCKEVVDSHPSVSGHDIAMSKSEN STSSK  LG SGK+GVYSTNGVQELLECP
Sbjct: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPC+YQ
Sbjct: 63  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCR+RPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT
Sbjct: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLG APVYMAFLRFMG+DNEAKK
Sbjct: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT
Sbjct: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 302

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 303 GRIWKEE 309

BLAST of ClCG04G000960.1 vs. ExPASy TrEMBL
Match: A0A6J1HT85 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467239 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 9.3e-178
Identity = 295/307 (96.09%), Postives = 301/307 (98.05%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPGSSTCKEVVDSHPSVSGHDIAMSKSEN STSSK  LG SGK+GVYSTNGVQELLECP
Sbjct: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPC+YQ
Sbjct: 63  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCR+RPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT
Sbjct: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLG APVYMAFLRFMG+DNEAKK
Sbjct: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELKLRIT
Sbjct: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRIT 302

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 303 GRIWKEE 309

BLAST of ClCG04G000960.1 vs. ExPASy TrEMBL
Match: A0A6J1EBG4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432512 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.3e-175
Identity = 291/307 (94.79%), Postives = 299/307 (97.39%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPGSSTCK+ VDSHPS+SGHDIA+SKSEN  T+SK  LGL GK+GVYSTNGVQELLECP
Sbjct: 1   MAPGSSTCKK-VDSHPSISGHDIAISKSENNPTTSKASLGLGGKLGVYSTNGVQELLECP 60

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VCTNLMYPPIYQCPNGHTLCSNCKIRVSN CPTCRHELGNIRCLALEKVAESLEIPC+YQ
Sbjct: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNSCPTCRHELGNIRCLALEKVAESLEIPCRYQ 120

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCR+RPYNCPYAGSECSVTGDI SLVAHLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIQSLVAHLKDDHKVDMHDGCT 180

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT
Sbjct: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 301 GRIWKEE 306

BLAST of ClCG04G000960.1 vs. ExPASy TrEMBL
Match: A0A6J1HS09 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466063 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 3.6e-174
Identity = 291/307 (94.79%), Postives = 298/307 (97.07%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPGSSTCK+ VDSH SVSGHDIA+SKSEN  T+SK  LGL GK+GVYSTNGVQELLECP
Sbjct: 1   MAPGSSTCKK-VDSHLSVSGHDIAISKSENNPTTSKASLGLGGKLGVYSTNGVQELLECP 60

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VCTNLMYPPIYQCPNGHTLCSNCKIRVSN CPTCRHELGNIRCLALEKVAESLEIPC+YQ
Sbjct: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNSCPTCRHELGNIRCLALEKVAESLEIPCRYQ 120

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCR+RPYNCPYAGSECSVTGDI SLVAHLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIQSLVAHLKDDHKVDMHDGCT 180

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT
Sbjct: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 301 GRIWKEE 306

BLAST of ClCG04G000960.1 vs. ExPASy TrEMBL
Match: A0A6J1DL03 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111022066 PE=3 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 5.8e-172
Identity = 287/315 (91.11%), Postives = 297/315 (94.29%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSE--------NISTSSKGPLGLSGKIGVYSTNG 60
           MAPG+S CKEVVDSHPSVSGHDIA+SKSE        + S S+K  LGL GK+GVYSTNG
Sbjct: 1   MAPGTSACKEVVDSHPSVSGHDIAVSKSESNSNSNSNSNSASTKVLLGLGGKLGVYSTNG 60

Query: 61  VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAES 120
           VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAES
Sbjct: 61  VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAES 120

Query: 121 LEIPCKYQNLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHK 180
           LEIPC+Y +LGC DIFPYYSKLKHEQHCR+RPYNCPYAGSECSV+GDIPSLV HLKDDHK
Sbjct: 121 LEIPCRYHSLGCLDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVSGDIPSLVTHLKDDHK 180

Query: 181 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFM 240
           VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLGMAPVYMAFLRFM
Sbjct: 181 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFM 240

Query: 241 GDDNEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER 300
           GDDNEAKKFSYSLEVGGNGRKLIW GIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER
Sbjct: 241 GDDNEAKKFSYSLEVGGNGRKLIWHGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER 300

Query: 301 QELKLRITGRIWKEE 308
           QELKLRITGRIWKEE
Sbjct: 301 QELKLRITGRIWKEE 315

BLAST of ClCG04G000960.1 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 549.3 bits (1414), Expect = 2.0e-156
Identity = 253/309 (81.88%), Postives = 277/309 (89.64%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSE--NISTSSKGPLGLSGKIGVYSTNGVQELLE 60
           MAPG S  KEV++S+ +   +++  +K E  N   +  G  G+ GK G++S NGV ELLE
Sbjct: 1   MAPGGSALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGI-GKYGIHSNNGVYELLE 60

Query: 61  CPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCK 120
           CPVCTNLMYPPI+QCPNGHTLCSNCK+RV N CPTCR+ELGNIRCLALEKVAESLE+PC+
Sbjct: 61  CPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCR 120

Query: 121 YQNLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180
           YQNLGC DIFPYYSKLKHEQHCR+RPY CPYAGSECSVTGDIP+LV HLKDDHKVDMHDG
Sbjct: 121 YQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDG 180

Query: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEA 240
           CTFNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLGMAPVYMAFLRFMGD+NEA
Sbjct: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEA 240

Query: 241 KKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLR 300
           KKFSYSLEVG +GRKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSGG+RQELKLR
Sbjct: 241 KKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLR 300

Query: 301 ITGRIWKEE 308
           +TGRIWKEE
Sbjct: 301 VTGRIWKEE 308

BLAST of ClCG04G000960.1 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 536.2 bits (1380), Expect = 1.7e-152
Identity = 247/307 (80.46%), Postives = 274/307 (89.25%), Query Frame = 0

Query: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECP 60
           MAPG S  KE ++S+ +   +++ M+K E  S  +K   G  GK   +S+NGV ELLECP
Sbjct: 1   MAPGGSALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKF--HSSNGVYELLECP 60

Query: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQ 120
           VCTNLMYPPI+QCPNGHTLCS+CK+RV N CPTCR+ELGNIRCLALEKVAESLE+PC+YQ
Sbjct: 61  VCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQ 120

Query: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180
           NLGCQDIFPYYSKLKHEQHCR+R Y+CPYAGSECSVTGDIP+LV HLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCT 180

Query: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLGMAPVYMAFLRFMGD+NEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKK 240

Query: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300
           FSYSLEVG + RKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSG +++ELKLR+T
Sbjct: 241 FSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVT 300

Query: 301 GRIWKEE 308
           GRIWKEE
Sbjct: 301 GRIWKEE 305

BLAST of ClCG04G000960.1 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 475.3 bits (1222), Expect = 3.6e-134
Identity = 218/294 (74.15%), Postives = 249/294 (84.69%), Query Frame = 0

Query: 14  SHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECPVCTNLMYPPIYQC 73
           S+ SVS H    + + N++ ++ G L         +T  V ELLECPVCTN MYPPI+QC
Sbjct: 26  SYASVSKHHHTNNNTTNVNAAASGLLP--------TTTSVHELLECPVCTNSMYPPIHQC 85

Query: 74  PNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQNLGCQDIFPYYSK 133
            NGHTLCS CK RV N CPTCR ELG+IRCLALEKVAESLE+PCK+ +LGC +IFPYYSK
Sbjct: 86  HNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFPYYSK 145

Query: 134 LKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEV 193
           LKHE  C +RPY+CPYAGSECSVTGDIP LVAHL+DDHKVDMH GCTFNHRYVKSNP EV
Sbjct: 146 LKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREV 205

Query: 194 ENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRK 253
           ENATWMLTVF+CFG+ FCLHFEAFQLGMAPVYMAFLRFMGD+ EA+ ++YSLEVGG GRK
Sbjct: 206 ENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGRK 265

Query: 254 LIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIWKEE 308
           LIW+G PR +RDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TGRIWKE+
Sbjct: 266 LIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 311

BLAST of ClCG04G000960.1 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 459.5 bits (1181), Expect = 2.1e-129
Identity = 209/303 (68.98%), Postives = 244/303 (80.53%), Query Frame = 0

Query: 5   SSTCKEVVDSHPSVSGHDIAMSKSENISTSSKGPLGLSGKIGVYSTNGVQELLECPVCTN 64
           SST  E+  +      +  + +K+   + ++     + G         V ELLECPVCT 
Sbjct: 10  SSTGNEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPTATAPATSVYELLECPVCTY 69

Query: 65  LMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCKYQNLGC 124
            MYPPI+QC NGHTLCS CK+RV N CPTCR ELG+IRCLALEKVAESLE+PCK+ NLGC
Sbjct: 70  SMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAESLELPCKFYNLGC 129

Query: 125 QDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHR 184
            +IFPYYSKLKHE  C +RPY+CPYAGSEC + GDIP LVAHL+DDHKVDMH G TFNHR
Sbjct: 130 PEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHKVDMHAGSTFNHR 189

Query: 185 YVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYS 244
           YVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGM PVYMAFLRFMGD+ +A+ +SYS
Sbjct: 190 YVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYS 249

Query: 245 LEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRITGRIW 304
           LEVGG+GRKL W+G PR IRDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TG+IW
Sbjct: 250 LEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIW 309

Query: 305 KEE 308
           KE+
Sbjct: 310 KEQ 312

BLAST of ClCG04G000960.1 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 366.7 bits (940), Expect = 1.8e-101
Identity = 161/204 (78.92%), Postives = 184/204 (90.20%), Query Frame = 0

Query: 104 LALEKVAESLEIPCKYQNLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSL 163
           +ALEKVAESLE+PCKY NLGC  IFPYYSKLKHE  C +RPY+CPYAGSEC+  GDI  L
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 164 VAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAP 223
           VAHL+DDHKVDMH GCTFNHRYVKSNP EVENATWMLTVF CFG+ FCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 224 VYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNL 283
           VYMAFLRFMGD+++A+ ++YSLEVGG+GRK  W+G PR +RDSHRKVRDS DGLIIQRN+
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 284 ALYFSGGERQELKLRITGRIWKEE 308
           AL+FSGG+++ELKLR+TGRIWKE+
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038881778.12.2e-18198.05E3 ubiquitin-protein ligase SINAT2-like [Benincasa hispida] >XP_038881779.1 E3 u... [more]
XP_022928730.16.6e-17896.42E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_0229... [more]
XP_022967856.11.9e-17796.09E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita maxima] >XP_023543... [more]
KAG6603259.19.5e-17795.77E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023518392.15.2e-17595.11E3 ubiquitin-protein ligase SINAT2-like [Cucurbita pepo subsp. pepo] >XP_0235183... [more]
Match NameE-valueIdentityDescription
Q9M2P42.8e-15581.88E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
P937482.4e-15180.46Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=S... [more]
Q10L913.6e-13480.00E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q84JL35.1e-13374.15E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q8S3N17.7e-12972.20E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1ESE83.2e-17896.42RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111435553... [more]
A0A6J1HT859.3e-17896.09RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467239 P... [more]
A0A6J1EBG43.3e-17594.79RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432512... [more]
A0A6J1HS093.6e-17494.79RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466063 P... [more]
A0A6J1DL035.8e-17291.11RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102206... [more]
Match NameE-valueIdentityDescription
AT3G58040.12.0e-15681.88seven in absentia of Arabidopsis 2 [more]
AT2G41980.11.7e-15280.46Protein with RING/U-box and TRAF-like domains [more]
AT3G61790.13.6e-13474.15Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.12.1e-12968.98Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.11.8e-10178.92TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 101..300
e-value: 2.9E-81
score: 272.2
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 27..113
e-value: 2.1E-7
score: 32.5
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 114..166
e-value: 6.2E-18
score: 66.8
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 167..300
e-value: 9.3E-38
score: 131.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availablePANTHERPTHR10315:SF123E3 UBIQUITIN-PROTEIN LIGASE SINAT2-LIKEcoord: 1..306
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 1..306
NoneNo IPR availableCDDcd03829Sinacoord: 173..301
e-value: 3.74455E-62
score: 191.446
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 57..95
e-value: 7.7793E-14
score: 62.6874
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 53..100
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 112..304
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 59..95
score: 9.420654
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 112..172
score: 14.526815

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
ClCG04G000960ClCG04G000960gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
ClCG04G000960.1-exonClCG04G000960.1-exon-CG_Chr04:3138412..3139704exon
ClCG04G000960.1-exonClCG04G000960.1-exon-CG_Chr04:3139926..3140229exon
ClCG04G000960.1-exonClCG04G000960.1-exon-CG_Chr04:3141747..3142133exon
ClCG04G000960.1-exonClCG04G000960.1-exon-CG_Chr04:3142823..3143471exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
ClCG04G000960.1-five_prime_utrClCG04G000960.1-five_prime_utr-CG_Chr04:3138412..3139704five_prime_UTR
ClCG04G000960.1-five_prime_utrClCG04G000960.1-five_prime_utr-CG_Chr04:3139926..3140013five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
ClCG04G000960.1-cdsClCG04G000960.1-cds-CG_Chr04:3140014..3140229CDS
ClCG04G000960.1-cdsClCG04G000960.1-cds-CG_Chr04:3141747..3142133CDS
ClCG04G000960.1-cdsClCG04G000960.1-cds-CG_Chr04:3142823..3143143CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
ClCG04G000960.1-three_prime_utrClCG04G000960.1-three_prime_utr-CG_Chr04:3143144..3143471three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
ClCG04G000960.1ClCG04G000960.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0061630 ubiquitin protein ligase activity