Chy5G096060.1 (mRNA) Cucumber (hystrix) v1

Overview
NameChy5G096060.1
TypemRNA
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPLASMODESMATA CALLOSE-BINDING PROTEIN 3
LocationchrH05: 6137469 .. 6139954 (+)
Sequence length390
RNA-Seq ExpressionChy5G096060.1
SyntenyChy5G096060.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACATTTCTTCTCTCCCACTACTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCATCGGAACCTCCACCGTCGACGGCGCGTACTGGTGCGTGGCCCGCAGCGACGCAACCTACGAATCTCTCCAAGCCGCCCTCGACTACGCCTGCGCCACCGGCGCTGACTGTACTCCGATTCTGTTGAACGGACTCTGTTTCCTCCCTAATACCATCCAGGCCCACGCCTCCTACGCCTTCAACAGCTTCTTCCAGCGCAAGGCTATGGCTCCCGGATCCTGTGACTTCGCCGGCTCCGCCACCATCGCCCAGAGTGACCCAAGTCCGTCCCCTCTCTTTACCTTCCTTCTCTGTTGTTCTTCTTGGTGTGCTTCAGACAGTTAGCTTTAAACTCTTTTTTTTTTCTTTTTTGCTCCTAATTTTAGCCTTATAAGGTATTTCCAAAGAAATTAATTTTTAAAATATAAAATAATAATAATAATAATAATTGTACTAAGATTGGATTAAAAATGCATTCATCTCAAATTTTTTTAAATGAAAAATTGATTCATTGTTGAACCATATTTTTCTCATGTTAAACTCAGTCCTTTTTCTACCTTTATCTTTAATATTGAAACATACTAAAATCCATTTGATTTTAATTCAAAAAATGTTTGTTACAATCAAAGTTGGTACAACTCATTATATGTATATATTAGGAATGATTTATATTAGTGTGGAAGATTGTTAGTAAAATCTTGTTTATTAAATTAATTTATAGAGTTACAAGTGTTAAAGAATGGTAGTGAAAGGGTAAAGTTTTGGTTTTGATTATTTTTCACATTGTTGTGTCTGTGCAGGTTATGGATCTTGTGTTTATCCATCTTCTCTAAGGTACACTAAATATTATGTTGTTATTATTTTCTTAAACGTGCATAGAGATTAATCTTTTATTATATTCTATTTTTACGAATATATTTAGTGGAGATTTCTTCATGTTCAAGTAAATGTATTCTCTTTAGAGTCTTCCGAAAATCCTATATTTGCTGTCTCCCATCCCCACTCAAATTAGTTTAAGGGAACAAACTTAGGTTTTTGAGTCGTTTCAATTCAATTGGTCAAATATCACTGTTACTTTAAATTCTAAATTAATAATTGTATCAATTTAAACTTTAAATTAATCGTTGTATTAATTTAAACCCTCAACTTTTATAAATATATCTCAATTAAACTTGAATTTATATAAATATATCAATTTAAACCTCTAATTTTCATAAGTGAATTCAAATAAAACATAATGGAGGATTTAAATTCATACAATTATTAGTTTGAGGTTTAAATTGATACCAGCCCTAAAAGTTAATTATTAGTTCAAGGTCTAAATAATTGATATAACCCTTATAGTTTAGGATGCATATTGATACTTTCTCAAATAGATAATAGAGAGAAAATAAGATATATTAATGAAAGAAATTAAAAAAAAAATGAAAATATAAAGTAATAAATGAGTGTTTTTTTTTTCAATTTCTCAAATAGATAACACTCATTTATTGCTTTATATTTTCATTTTTTTTTAACTTTCAAATATAGTACCATAGATAAAAATATTTACAAAATATGAAAAAATATTAAAACTAAATCGACTATTATTTGGTTCTATCGTAGTCTTTTCATTTAAAATAATATTCTCTCTAAAATTAAGAGTTATTTTTTAACAAGTTATTAAATAAAATACTTGATTTTGTTTTGAATTTTAACATTGTGTAATACACTTATTAATATATAATTATTTTACTAATAAAATAAATGAATTAAAATGGAGTGATTTTGTTTATAAGGGTCTAGAAGTTGAATAGGCCAATTTAAGTTTTAAGATAAAAGAAAGTTTACGTTCCTCCATATCTTCTTCCATTGGAGGAGGTTTGATTGATCAAATATGGGCATAAGAAGAGAACAATGCCTCTAATCTTTAAGAATACGAGAATTTCACCCAAAGAATTTGAAGTGATGGAGTTTGTAAGGTAAACCCTAAACTTTACCATTTTCTCCAAAATTCTCATTCTTTCACCATCTACTTTAAAATAAAGATTCTACAACTAAATCATTTCTTGCACAGAAAATACACAATTTTCTGTCCTTTTTTTTTGTTTTTTTTGTACCATTTTTATAACTTGTCAAAAGAGGGAATTTAAAAGAGTGGATAAATTATAGGCTGAATTACTTTCATTGAAAAAAAGCATAAACTTTACTAATTGTTTCTAAAATATTGTTATTTAAAAAAAAAAAAAAAAAAAAGTTGCAATATTACATATCATATCTACATCGTCCTGTCTTACAAAACGACATGCGAGTGACTAGGACATTGAAGAGAATTTTAAAACTTTTAAAAAAATTGTAAATATTGCAATTTTTTGTTGGAGGGGCCTGATTTCAAAGCTAATTAATCAGTATTGTTTTATTTATTGATTTGAAGGAAAGAGAAGGGTAGTAATTGGAAGGGATTGTAG

mRNA sequence

ATGGCCACATTTCTTCTCTCCCACTACTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCATCGGAACCTCCACCGTCGACGGCGCGTACTGGTGCGTGGCCCGCAGCGACGCAACCTACGAATCTCTCCAAGCCGCCCTCGACTACGCCTGCGCCACCGGCGCTGACTGTACTCCGATTCTGTTGAACGGACTCTGTTTCCTCCCTAATACCATCCAGGCCCACGCCTCCTACGCCTTCAACAGCTTCTTCCAGCGCAAGGCTATGGCTCCCGGATCCTGTGACTTCGCCGGCTCCGCCACCATCGCCCAGAGTGACCCAAGTTATGGATCTTGTGTTTATCCATCTTCTCTAAGGAAAGAGAAGGGTAGTAATTGGAAGGGATTGTAG

Coding sequence (CDS)

ATGGCCACATTTCTTCTCTCCCACTACTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCATCGGAACCTCCACCGTCGACGGCGCGTACTGGTGCGTGGCCCGCAGCGACGCAACCTACGAATCTCTCCAAGCCGCCCTCGACTACGCCTGCGCCACCGGCGCTGACTGTACTCCGATTCTGTTGAACGGACTCTGTTTCCTCCCTAATACCATCCAGGCCCACGCCTCCTACGCCTTCAACAGCTTCTTCCAGCGCAAGGCTATGGCTCCCGGATCCTGTGACTTCGCCGGCTCCGCCACCATCGCCCAGAGTGACCCAAGTTATGGATCTTGTGTTTATCCATCTTCTCTAAGGAAAGAGAAGGGTAGTAATTGGAAGGGATTGTAG

Protein sequence

MATFLLSHYSFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLRKEKGSNWKGL*
Homology
BLAST of Chy5G096060.1 vs. ExPASy Swiss-Prot
Match: Q9FNQ2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 4.5e-22
Identity = 48/105 (45.71%), Postives = 64/105 (60.95%), Query Frame = 0

Query: 20  LIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPILLNGLCFLPNTIQAHASYA 79
           L+  +    A WCV ++  +   LQA LDYAC  GADC P      CF P+ +++H +YA
Sbjct: 10  LLSLAGHSSASWCVCKTGLSDTVLQATLDYACGNGADCNPTKPKQSCFNPDNVRSHCNYA 69

Query: 80  FNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLRKEKGS 125
            NSFFQ+K  +PGSC+F G+AT   SDPSY  C +P+S     GS
Sbjct: 70  VNSFFQKKGQSPGSCNFDGTATPTNSDPSYTGCAFPTSASGSSGS 114

BLAST of Chy5G096060.1 vs. ExPASy Swiss-Prot
Match: Q9FZ86 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 5.9e-22
Identity = 50/104 (48.08%), Postives = 65/104 (62.50%), Query Frame = 0

Query: 14  FFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPILLNGLCFLPNTIQ 73
           F     L+  +      WCV +   +   LQ  LDYAC  GADC PI   G CF PNT++
Sbjct: 4   FVLVMILLAMAGHSSGTWCVCKEGLSEAMLQKTLDYACGAGADCGPIHQTGPCFNPNTVK 63

Query: 74  AHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSS 118
           +H SYA NSFFQ+K  + G+CDFAG+AT + SDPSY +C +P+S
Sbjct: 64  SHCSYAVNSFFQKKGQSLGTCDFAGTATFSASDPSYTTCPFPAS 107

BLAST of Chy5G096060.1 vs. ExPASy Swiss-Prot
Match: Q9SD84 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.0e-21
Identity = 47/96 (48.96%), Postives = 60/96 (62.50%), Query Frame = 0

Query: 29  AYWCVARSDATYESLQAALDYACATGADCTPILLNGLCFLPNTIQAHASYAFNSFFQRKA 88
           A WCV ++  +   LQ  LDYAC  GADC P    G CF P+ ++AH +YA NSFFQ+K 
Sbjct: 19  ASWCVCKTGLSDSVLQKTLDYACGNGADCNPTHPKGSCFNPDNVRAHCNYAVNSFFQKKG 78

Query: 89  MAPGSCDFAGSATIAQSDPSYGSCVYPSSLRKEKGS 125
            A  SC+F G+AT+  +DPSY  C +PSS     GS
Sbjct: 79  QASESCNFTGTATLTTTDPSYTGCAFPSSASGSSGS 114

BLAST of Chy5G096060.1 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 5.5e-20
Identity = 43/90 (47.78%), Postives = 57/90 (63.33%), Query Frame = 0

Query: 26  VDGAYWCVARSDATYESLQAALDYACATGADCTPILLNGLCFLPNTIQAHASYAFNSFFQ 85
           V  + WCV +  AT E LQA+LD+AC  G DC  I   G CF PN + +HA+YA N +FQ
Sbjct: 359 VTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQ 418

Query: 86  RKAMAPGSCDFAGSATIAQSDPSYGSCVYP 116
           +    P  CDF+ +AT+   +PSY +CVYP
Sbjct: 419 KSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

BLAST of Chy5G096060.1 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.6e-19
Identity = 45/87 (51.72%), Postives = 59/87 (67.82%), Query Frame = 0

Query: 31  WCVARSDATYESLQAALDYACA-TGADCTPILLNGLCFLPNTIQAHASYAFNSFFQRKAM 90
           WCV +++AT   LQ+ +DY C+ +G DC PI  NG CF PNT++AHASYA NS++Q K  
Sbjct: 37  WCVPKAEATDAQLQSNIDYVCSQSGMDCGPIQANGACFNPNTVRAHASYAMNSWYQSKGR 96

Query: 91  APGSCDFAGSATIAQSDPSYGSCVYPS 117
               CDF+G+  I  SDPS GSC + S
Sbjct: 97  NDFDCDFSGTGAITSSDPSNGSCSFLS 123

BLAST of Chy5G096060.1 vs. ExPASy TrEMBL
Match: A0A0A0LND4 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G139290 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.7e-56
Identity = 115/124 (92.74%), Postives = 118/124 (95.16%), Query Frame = 0

Query: 1   MATFLLSHYSFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPI 60
           MATFLLSHYS FFFFFFFFL GTSTVDGAYWCVARSDATYESLQAALDYACATGA+CTPI
Sbjct: 1   MATFLLSHYS-FFFFFFFFLFGTSTVDGAYWCVARSDATYESLQAALDYACATGAECTPI 60

Query: 61  LLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLRK 120
           +LNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSL  
Sbjct: 61  MLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLSS 120

Query: 121 EKGS 125
             G+
Sbjct: 121 AGGA 123

BLAST of Chy5G096060.1 vs. ExPASy TrEMBL
Match: A0A1S3C4M3 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo OX=3656 GN=LOC103496965 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.6e-54
Identity = 113/125 (90.40%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 1   MATFLLSH-YSFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTP 60
           MATFL SH YSFF FFF FFLIGTS VDGAYWCVARSDATYESLQAA+DYACATGADCTP
Sbjct: 1   MATFLPSHYYSFFLFFFLFFLIGTSAVDGAYWCVARSDATYESLQAAIDYACATGADCTP 60

Query: 61  ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLR 120
           ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDF GSATIAQSDPSYGSCVYPSSL 
Sbjct: 61  ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFGGSATIAQSDPSYGSCVYPSSLS 120

Query: 121 KEKGS 125
              G+
Sbjct: 121 SAGGA 125

BLAST of Chy5G096060.1 vs. ExPASy TrEMBL
Match: A0A5A7SQ29 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold275G001060 PE=4 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.2e-48
Identity = 105/119 (88.24%), Postives = 106/119 (89.08%), Query Frame = 0

Query: 1   MATFLLSH-YSFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTP 60
           MATFL SH YSFF FFF FFLIGTS VDGAYWCVARSDATYESLQAA+DYACATGADCTP
Sbjct: 1   MATFLPSHYYSFFLFFFLFFLIGTSAVDGAYWCVARSDATYESLQAAIDYACATGADCTP 60

Query: 61  ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSL 119
           ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDF GSATIAQSDPS     Y  SL
Sbjct: 61  ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFGGSATIAQSDPSLSPSPYLPSL 119

BLAST of Chy5G096060.1 vs. ExPASy TrEMBL
Match: A0A6J1H812 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460407 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.8e-45
Identity = 97/124 (78.23%), Postives = 106/124 (85.48%), Query Frame = 0

Query: 1   MATFLLSHYSFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPI 60
           MAT +LSH   F      FL+ TS VDGAYWCVARS+A+YE+LQ ALDYACATGADCTP+
Sbjct: 1   MATLVLSH--SFLLLLLLFLLPTSAVDGAYWCVARSEASYEALQTALDYACATGADCTPL 60

Query: 61  LLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLRK 120
           L NGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQ+DPSYGSCVYPSS+  
Sbjct: 61  LSNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQTDPSYGSCVYPSSISA 120

Query: 121 EKGS 125
             G+
Sbjct: 121 AGGA 122

BLAST of Chy5G096060.1 vs. ExPASy TrEMBL
Match: A0A6J1KVB1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucurbita maxima OX=3661 GN=LOC111499021 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.3e-45
Identity = 96/124 (77.42%), Postives = 106/124 (85.48%), Query Frame = 0

Query: 1   MATFLLSHYSFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPI 60
           MAT +LSH   F      FL+ TS VDGAYWCVARS+A+YE+LQ ALDYACATGADCTP+
Sbjct: 1   MATLVLSH--SFLLLLLLFLLPTSAVDGAYWCVARSEASYEALQTALDYACATGADCTPL 60

Query: 61  LLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLRK 120
           L NGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSAT+AQ+DPSYGSCVYPSS+  
Sbjct: 61  LSNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATVAQTDPSYGSCVYPSSISA 120

Query: 121 EKGS 125
             G+
Sbjct: 121 AGGA 122

BLAST of Chy5G096060.1 vs. NCBI nr
Match: XP_004138961.2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis sativus])

HSP 1 Score: 229 bits (584), Expect = 3.41e-74
Identity = 115/124 (92.74%), Postives = 118/124 (95.16%), Query Frame = 0

Query: 1   MATFLLSHYSFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPI 60
           MATFLLSHYSFF FFFFFFL GTSTVDGAYWCVARSDATYESLQAALDYACATGA+CTPI
Sbjct: 1   MATFLLSHYSFF-FFFFFFLFGTSTVDGAYWCVARSDATYESLQAALDYACATGAECTPI 60

Query: 61  LLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLRK 120
           +LNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSL  
Sbjct: 61  MLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLSS 120

Query: 121 EKGS 124
             G+
Sbjct: 121 AGGA 123

BLAST of Chy5G096060.1 vs. NCBI nr
Match: KAE8651770.1 (hypothetical protein Csa_006265 [Cucumis sativus])

HSP 1 Score: 229 bits (584), Expect = 8.51e-74
Identity = 115/124 (92.74%), Postives = 118/124 (95.16%), Query Frame = 0

Query: 1   MATFLLSHYSFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPI 60
           MATFLLSHYSFF FFFFFFL GTSTVDGAYWCVARSDATYESLQAALDYACATGA+CTPI
Sbjct: 29  MATFLLSHYSFF-FFFFFFLFGTSTVDGAYWCVARSDATYESLQAALDYACATGAECTPI 88

Query: 61  LLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLRK 120
           +LNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSL  
Sbjct: 89  MLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLSS 148

Query: 121 EKGS 124
             G+
Sbjct: 149 AGGA 151

BLAST of Chy5G096060.1 vs. NCBI nr
Match: XP_008457240.1 (PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo])

HSP 1 Score: 223 bits (567), Expect = 1.41e-71
Identity = 113/125 (90.40%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 1   MATFLLSHY-SFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTP 60
           MATFL SHY SFF FFF FFLIGTS VDGAYWCVARSDATYESLQAA+DYACATGADCTP
Sbjct: 1   MATFLPSHYYSFFLFFFLFFLIGTSAVDGAYWCVARSDATYESLQAAIDYACATGADCTP 60

Query: 61  ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLR 120
           ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDF GSATIAQSDPSYGSCVYPSSL 
Sbjct: 61  ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFGGSATIAQSDPSYGSCVYPSSLS 120

Query: 121 KEKGS 124
              G+
Sbjct: 121 SAGGA 125

BLAST of Chy5G096060.1 vs. NCBI nr
Match: XP_038875835.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Benincasa hispida])

HSP 1 Score: 206 bits (523), Expect = 1.08e-64
Identity = 104/124 (83.87%), Postives = 108/124 (87.10%), Query Frame = 0

Query: 1   MATFLLSHYSFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPI 60
           MAT + SH     F  FFFLIGTS VDGAYWCVARSDA+YE+LQAALDYACATGADC PI
Sbjct: 23  MATLVFSHS----FLLFFFLIGTSAVDGAYWCVARSDASYEALQAALDYACATGADCNPI 82

Query: 61  LLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLRK 120
           LLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSS   
Sbjct: 83  LLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSTSA 142

Query: 121 EKGS 124
             G+
Sbjct: 143 AGGA 142

BLAST of Chy5G096060.1 vs. NCBI nr
Match: KAA0033304.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo var. makuwa] >TYK21601.1 PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo var. makuwa])

HSP 1 Score: 202 bits (514), Expect = 1.37e-64
Identity = 105/119 (88.24%), Postives = 106/119 (89.08%), Query Frame = 0

Query: 1   MATFLLSHY-SFFFFFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTP 60
           MATFL SHY SFF FFF FFLIGTS VDGAYWCVARSDATYESLQAA+DYACATGADCTP
Sbjct: 1   MATFLPSHYYSFFLFFFLFFLIGTSAVDGAYWCVARSDATYESLQAAIDYACATGADCTP 60

Query: 61  ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSL 118
           ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDF GSATIAQSDPS     Y  SL
Sbjct: 61  ILLNGLCFLPNTIQAHASYAFNSFFQRKAMAPGSCDFGGSATIAQSDPSLSPSPYLPSL 119

BLAST of Chy5G096060.1 vs. TAIR 10
Match: AT4G13600.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 158.3 bits (399), Expect = 4.2e-39
Identity = 75/107 (70.09%), Postives = 87/107 (81.31%), Query Frame = 0

Query: 18  FFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPILLNGLCFLPNTIQAHAS 77
           F L+       A WCVAR D T ++LQAALDYACA GADC PI  NGLCFLPNT+QAHAS
Sbjct: 48  FLLLLLFRSSAAMWCVARFDVTSQALQAALDYACAAGADCAPIQPNGLCFLPNTVQAHAS 107

Query: 78  YAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLRKEKGS 125
           YAFNS+FQR AMAPGSC+FAG++TIA++DPSYGSCVYP+S+R   GS
Sbjct: 108 YAFNSYFQRAAMAPGSCNFAGTSTIAKTDPSYGSCVYPNSVRNAGGS 154

BLAST of Chy5G096060.1 vs. TAIR 10
Match: AT5G61130.1 (plasmodesmata callose-binding protein 1 )

HSP 1 Score: 105.5 bits (262), Expect = 3.2e-23
Identity = 48/105 (45.71%), Postives = 64/105 (60.95%), Query Frame = 0

Query: 20  LIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPILLNGLCFLPNTIQAHASYA 79
           L+  +    A WCV ++  +   LQA LDYAC  GADC P      CF P+ +++H +YA
Sbjct: 10  LLSLAGHSSASWCVCKTGLSDTVLQATLDYACGNGADCNPTKPKQSCFNPDNVRSHCNYA 69

Query: 80  FNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSSLRKEKGS 125
            NSFFQ+K  +PGSC+F G+AT   SDPSY  C +P+S     GS
Sbjct: 70  VNSFFQKKGQSPGSCNFDGTATPTNSDPSYTGCAFPTSASGSSGS 114

BLAST of Chy5G096060.1 vs. TAIR 10
Match: AT1G18650.1 (plasmodesmata callose-binding protein 3 )

HSP 1 Score: 105.1 bits (261), Expect = 4.2e-23
Identity = 50/104 (48.08%), Postives = 65/104 (62.50%), Query Frame = 0

Query: 14  FFFFFFLIGTSTVDGAYWCVARSDATYESLQAALDYACATGADCTPILLNGLCFLPNTIQ 73
           F     L+  +      WCV +   +   LQ  LDYAC  GADC PI   G CF PNT++
Sbjct: 4   FVLVMILLAMAGHSSGTWCVCKEGLSEAMLQKTLDYACGAGADCGPIHQTGPCFNPNTVK 63

Query: 74  AHASYAFNSFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPSS 118
           +H SYA NSFFQ+K  + G+CDFAG+AT + SDPSY +C +P+S
Sbjct: 64  SHCSYAVNSFFQKKGQSLGTCDFAGTATFSASDPSYTTCPFPAS 107

BLAST of Chy5G096060.1 vs. TAIR 10
Match: AT5G08000.1 (glucan endo-1,3-beta-glucosidase-like protein 3 )

HSP 1 Score: 104.4 bits (259), Expect = 7.2e-23
Identity = 47/96 (48.96%), Postives = 60/96 (62.50%), Query Frame = 0

Query: 29  AYWCVARSDATYESLQAALDYACATGADCTPILLNGLCFLPNTIQAHASYAFNSFFQRKA 88
           A WCV ++  +   LQ  LDYAC  GADC P    G CF P+ ++AH +YA NSFFQ+K 
Sbjct: 19  ASWCVCKTGLSDSVLQKTLDYACGNGADCNPTHPKGSCFNPDNVRAHCNYAVNSFFQKKG 78

Query: 89  MAPGSCDFAGSATIAQSDPSYGSCVYPSSLRKEKGS 125
            A  SC+F G+AT+  +DPSY  C +PSS     GS
Sbjct: 79  QASESCNFTGTATLTTTDPSYTGCAFPSSASGSSGS 114

BLAST of Chy5G096060.1 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 101.3 bits (251), Expect = 6.1e-22
Identity = 46/95 (48.42%), Postives = 62/95 (65.26%), Query Frame = 0

Query: 23  TSTVDGAYWCVARSDATYESLQAALDYACAT-GADCTPILLNGLCFLPNTIQAHASYAFN 82
           + +++G  WCVA  DA  E LQ  LDYAC   GADC PI     C+ P+T++AHAS+AFN
Sbjct: 376 SKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYSPDTLEAHASFAFN 435

Query: 83  SFFQRKAMAPGSCDFAGSATIAQSDPSYGSCVYPS 117
           S++Q+K  A GSC F G+A +    P YG C +P+
Sbjct: 436 SYYQKKGRAGGSCYFGGAAYVVSQPPKYGRCEFPT 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FNQ24.5e-2245.71PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1... [more]
Q9FZ865.9e-2248.08PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3... [more]
Q9SD841.0e-2148.96PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2... [more]
Q9M0695.5e-2047.78Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q84V391.6e-1951.72Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LND41.7e-5692.74X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G139290 PE=4 SV=... [more]
A0A1S3C4M31.6e-5490.40PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo OX=3656 GN=LOC103496965 ... [more]
A0A5A7SQ292.2e-4888.24PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1H8121.8e-4578.23PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KVB12.3e-4577.42PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Cucurbita maxima OX=3661 GN=LOC111499... [more]
Match NameE-valueIdentityDescription
XP_004138961.23.41e-7492.74PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis sativus][more]
KAE8651770.18.51e-7492.74hypothetical protein Csa_006265 [Cucumis sativus][more]
XP_008457240.11.41e-7190.40PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo][more]
XP_038875835.11.08e-6483.87PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Benincasa hispida][more]
KAA0033304.11.37e-6488.24PLASMODESMATA CALLOSE-BINDING PROTEIN 3 [Cucumis melo var. makuwa] >TYK21601.1 P... [more]
Match NameE-valueIdentityDescription
AT4G13600.14.2e-3970.09Carbohydrate-binding X8 domain superfamily protein [more]
AT5G61130.13.2e-2345.71plasmodesmata callose-binding protein 1 [more]
AT1G18650.14.2e-2348.08plasmodesmata callose-binding protein 3 [more]
AT5G08000.17.2e-2348.96glucan endo-1,3-beta-glucosidase-like protein 3 [more]
AT2G05790.16.1e-2248.42O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 30..114
e-value: 3.3E-42
score: 156.2
IPR012946X8 domainPFAMPF07983X8coord: 31..101
e-value: 4.2E-20
score: 72.2
NoneNo IPR availableGENE3D1.20.58.1040coord: 24..124
e-value: 3.1E-10
score: 42.3
NoneNo IPR availablePANTHERPTHR31044:SF47CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEINcoord: 16..120
IPR044788Carbohydrate-binding X8 domain-containing protein, plantPANTHERPTHR31044BETA-1,3 GLUCANASEcoord: 16..120

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Chy5G096060Chy5G096060gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Chy5G096060.1.exon1Chy5G096060.1.exon1exon
Chy5G096060.1.exon2Chy5G096060.1.exon2exon
Chy5G096060.1.exon3Chy5G096060.1.exon3exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Chy5G096060.1.cds1Chy5G096060.1.cds1CDS
Chy5G096060.1.cds2Chy5G096060.1.cds2CDS
Chy5G096060.1.cds3Chy5G096060.1.cds3CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Chy5G096060.1Chy5G096060.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009506 plasmodesma
molecular_function GO:0016787 hydrolase activity