Chy12G209900.1 (mRNA) Cucumber (hystrix) v1

Overview


Name	Chy12G209900.1
Type	mRNA
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Pectinesterase
Location	chrH12: 4598161 .. 4600215 (-)
Sequence length	1704
RNA-Seq Expression	Chy12G209900.1
Synteny	Chy12G209900.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTTCTTCGACCTTACGGTAAGGTCGATGAAACCGACCAATTGAGGCTCGACGCTAGCCGGAGGACTCGTAGGAGGATTGTTGTCATTGTTTTATCCTCTGTTGTTCTTGTTGCTGTTGTTGTTGCTGTTGTTTTTGGTACTAGAAATGCTTCTAATAACAATGATGACAGTAACAATCAAAATGGTTCTCTTTCAACTTCAATCAAAGCAGTTTGTGATGTGACATTGTATCCTGATACATGTCAAAAGGCTTTTGGTCCAATAGCTAACTCGAGCCACCTCGATCCCGGTCAAATATCGAAGCTTTCGGTTCAATTGGCTCTCGGTGAATTATCAAAAGTGGCTAACTACATTTTTGATCATGCTATTACCAATACAGCTGACAACAAGACCATATTGGCTTTGAAAAATTGTCATGAACTTTTGGACTTAGCTTTGGATCATTTGAATATCTCATTGTCTTCTAGTGATATTACGTTACTGAAAGCTGTGGATGATTTGAAAACATGGATAACTTCAGCAGCCACTTACCAACAGACTTGCATCGATGGTCTTGCTGAAGTCGATCCTGCTTTGGCAGACCTCGTTGCAAATTTCTTGAAGAACTCCACTGAGTTGACTAGTAATGGACTTGCCATTGTGTCGTTCTTTTCGAAGCTAACGGATTCTTTAAGTCTACGACGATTGATGAGTTATGAAGACCATCAAAGCTATGGAGATTGGGCACGTCCAATTCTCCGAAAGCTGGCTCAAAGTGATATCAGGAAGCAGGCTGACATTGTTGTAGCGAAAGACCGTTCGGGGAAGTACAAAACAATCACTGAAGCTCTCAAGGCTGTTCCTGATAAGAGCAAGAAGAGAACTGTAATTTATGTGAAGAAGGGAACTTACAAGGAGAACGTTGAAGTTGTGAAGAACAAATGGAATGTGGTTATGGTTGGCGATGGAATGAATGCAACCATAGTAAGTGGCAACCTTAATGTCGTAGATGGAACACCAACATTTTCAACTGCAACATTTGGTAAGAAAACCAAAAGATTCATCTAAGCTTATAATTTGAGGTTTTTGGGTTCTAAATTGGTTGTTATAGTTTGTGGGATATAATAGTTTGTGTTTGAGGAGTAGAATATTTCAATCTAGGTTATAGTATCTTGGTTACAAATGAGGAAAGAGAATGGCTACAAAATCGCAAACGCTATAGATAATGAGAGGTTTCAAATAGTATTACTGTAGCTATGCATGGATTATAATGAGAAAAGGATACCGCACCTTATCTAAGGCAAGTTGGGGGCCAAAGCCTTTTAGTTTTTTACAAACCTTACAATCTAACAATTTGTTTCCATGTGTTTTTTTTTTTTTTTTTCTGTTGCAGCTGCGAAGGGAAAAGGCTTCATTGCTATAGACATGGGATTCATAAACACGGCTGGACCGAGCAAACACCAAGCAGTCGCTCTGATGTCAACATCTGACCAATCGATCTTCTACCGTTGTGAAATGAATGCATACCAAGACACTCTCTACGCACACTCCAACCGTCAATTCTACCGTGAATGTAAGGTTTACGGCACAGTTGATTTCATCTTTGGAAACTCGGCAGTAGTCCTACAAAACTGCACCATTGCACCAAGGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCAGGTTTGATCCTAATCAAAACACTGGCATTTCGATACAAGGTTCTTTGATCCAACCGTTCGACGACTTGAAAACAACCGAAACTTACCTCGGTCGTCCATGGAAGAACTACTCGACGACCGTGTTTATGCAAAGTAATTTTGGGAGGTTGATTCACCCAACTGGTTGGCTGCCATGGGTTGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTTCAAAATTATGGCCCTGGTTCTTCCACATCCAAAAGAGTTAAATGGAAGGGTGTCAAGAACATTGACATTAAAACTGCCAAAAAGTTCACAGTTTCTTCCTTCATTGATGGTAAAGATTGGATCTCAAAGGCACAAGTTCCCTTCACTGCCACTCTCTGA

mRNA sequence

ATGTCTTTTCTTCGACCTTACGGTAAGGTCGATGAAACCGACCAATTGAGGCTCGACGCTAGCCGGAGGACTCGTAGGAGGATTGTTGTCATTGTTTTATCCTCTGTTGTTCTTGTTGCTGTTGTTGTTGCTGTTGTTTTTGGTACTAGAAATGCTTCTAATAACAATGATGACAGTAACAATCAAAATGGTTCTCTTTCAACTTCAATCAAAGCAGTTTGTGATGTGACATTGTATCCTGATACATGTCAAAAGGCTTTTGGTCCAATAGCTAACTCGAGCCACCTCGATCCCGGTCAAATATCGAAGCTTTCGGTTCAATTGGCTCTCGGTGAATTATCAAAAGTGGCTAACTACATTTTTGATCATGCTATTACCAATACAGCTGACAACAAGACCATATTGGCTTTGAAAAATTGTCATGAACTTTTGGACTTAGCTTTGGATCATTTGAATATCTCATTGTCTTCTAGTGATATTACGTTACTGAAAGCTGTGGATGATTTGAAAACATGGATAACTTCAGCAGCCACTTACCAACAGACTTGCATCGATGGTCTTGCTGAAGTCGATCCTGCTTTGGCAGACCTCGTTGCAAATTTCTTGAAGAACTCCACTGAGTTGACTAGTAATGGACTTGCCATTGTGTCGTTCTTTTCGAAGCTAACGGATTCTTTAAGTCTACGACGATTGATGAGTTATGAAGACCATCAAAGCTATGGAGATTGGGCACGTCCAATTCTCCGAAAGCTGGCTCAAAGTGATATCAGGAAGCAGGCTGACATTGTTGTAGCGAAAGACCGTTCGGGGAAGTACAAAACAATCACTGAAGCTCTCAAGGCTGTTCCTGATAAGAGCAAGAAGAGAACTGTAATTTATGTGAAGAAGGGAACTTACAAGGAGAACGTTGAAGTTGTGAAGAACAAATGGAATGTGGTTATGGTTGGCGATGGAATGAATGCAACCATAGTAAGTGGCAACCTTAATGTCGTAGATGGAACACCAACATTTTCAACTGCAACATTTGCTGCGAAGGGAAAAGGCTTCATTGCTATAGACATGGGATTCATAAACACGGCTGGACCGAGCAAACACCAAGCAGTCGCTCTGATGTCAACATCTGACCAATCGATCTTCTACCGTTGTGAAATGAATGCATACCAAGACACTCTCTACGCACACTCCAACCGTCAATTCTACCGTGAATGTAAGGTTTACGGCACAGTTGATTTCATCTTTGGAAACTCGGCAGTAGTCCTACAAAACTGCACCATTGCACCAAGGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCAGGTTTGATCCTAATCAAAACACTGGCATTTCGATACAAGGTTCTTTGATCCAACCGTTCGACGACTTGAAAACAACCGAAACTTACCTCGGTCGTCCATGGAAGAACTACTCGACGACCGTGTTTATGCAAAGTAATTTTGGGAGGTTGATTCACCCAACTGGTTGGCTGCCATGGGTTGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTTCAAAATTATGGCCCTGGTTCTTCCACATCCAAAAGAGTTAAATGGAAGGGTGTCAAGAACATTGACATTAAAACTGCCAAAAAGTTCACAGTTTCTTCCTTCATTGATGGTAAAGATTGGATCTCAAAGGCACAAGTTCCCTTCACTGCCACTCTCTGA

Coding sequence (CDS)

ATGTCTTTTCTTCGACCTTACGGTAAGGTCGATGAAACCGACCAATTGAGGCTCGACGCTAGCCGGAGGACTCGTAGGAGGATTGTTGTCATTGTTTTATCCTCTGTTGTTCTTGTTGCTGTTGTTGTTGCTGTTGTTTTTGGTACTAGAAATGCTTCTAATAACAATGATGACAGTAACAATCAAAATGGTTCTCTTTCAACTTCAATCAAAGCAGTTTGTGATGTGACATTGTATCCTGATACATGTCAAAAGGCTTTTGGTCCAATAGCTAACTCGAGCCACCTCGATCCCGGTCAAATATCGAAGCTTTCGGTTCAATTGGCTCTCGGTGAATTATCAAAAGTGGCTAACTACATTTTTGATCATGCTATTACCAATACAGCTGACAACAAGACCATATTGGCTTTGAAAAATTGTCATGAACTTTTGGACTTAGCTTTGGATCATTTGAATATCTCATTGTCTTCTAGTGATATTACGTTACTGAAAGCTGTGGATGATTTGAAAACATGGATAACTTCAGCAGCCACTTACCAACAGACTTGCATCGATGGTCTTGCTGAAGTCGATCCTGCTTTGGCAGACCTCGTTGCAAATTTCTTGAAGAACTCCACTGAGTTGACTAGTAATGGACTTGCCATTGTGTCGTTCTTTTCGAAGCTAACGGATTCTTTAAGTCTACGACGATTGATGAGTTATGAAGACCATCAAAGCTATGGAGATTGGGCACGTCCAATTCTCCGAAAGCTGGCTCAAAGTGATATCAGGAAGCAGGCTGACATTGTTGTAGCGAAAGACCGTTCGGGGAAGTACAAAACAATCACTGAAGCTCTCAAGGCTGTTCCTGATAAGAGCAAGAAGAGAACTGTAATTTATGTGAAGAAGGGAACTTACAAGGAGAACGTTGAAGTTGTGAAGAACAAATGGAATGTGGTTATGGTTGGCGATGGAATGAATGCAACCATAGTAAGTGGCAACCTTAATGTCGTAGATGGAACACCAACATTTTCAACTGCAACATTTGCTGCGAAGGGAAAAGGCTTCATTGCTATAGACATGGGATTCATAAACACGGCTGGACCGAGCAAACACCAAGCAGTCGCTCTGATGTCAACATCTGACCAATCGATCTTCTACCGTTGTGAAATGAATGCATACCAAGACACTCTCTACGCACACTCCAACCGTCAATTCTACCGTGAATGTAAGGTTTACGGCACAGTTGATTTCATCTTTGGAAACTCGGCAGTAGTCCTACAAAACTGCACCATTGCACCAAGGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCAGGTTTGATCCTAATCAAAACACTGGCATTTCGATACAAGGTTCTTTGATCCAACCGTTCGACGACTTGAAAACAACCGAAACTTACCTCGGTCGTCCATGGAAGAACTACTCGACGACCGTGTTTATGCAAAGTAATTTTGGGAGGTTGATTCACCCAACTGGTTGGCTGCCATGGGTTGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTTCAAAATTATGGCCCTGGTTCTTCCACATCCAAAAGAGTTAAATGGAAGGGTGTCAAGAACATTGACATTAAAACTGCCAAAAAGTTCACAGTTTCTTCCTTCATTGATGGTAAAGATTGGATCTCAAAGGCACAAGTTCCCTTCACTGCCACTCTCTGA

Protein sequence

MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYIFDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQQTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSYGDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYKENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYSTTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKTAKKFTVSSFIDGKDWISKAQVPFTATL*

Homology

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BLAST of Chy12G209900.1 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 5.3e-168
Identity = 311/568 (54.75%), Postives = 398/568 (70.07%), Query Frame = 0


Query: 6   PYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGT--RNASNNNDDSNNQN 65
           PYGKVDE + +RL+A R+TR+ I +I +S V+L  +V+  VFGT     S    ++NN  
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  GSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYIFDH 125
            S+S S+KAVCDVTL+ + C +  G   N+S L+P ++ + +V++ + E+SK  N     
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAINAF--- 123

Query: 126 AITNTADNKTILALKNCHELLDLALDHLNISLSSS---DITLLKAVDDLKTWITSAATYQ 185
             ++  D K  + +  C ELLDL +D+LN +L+SS   D+T+ + VDDL+TW++SA TYQ
Sbjct: 124 -SSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTYQ 183

Query: 186 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL-RRLMSYEDHQS 245
           +TC++ LA   P +     + LKNSTELTSN LAI+++  K+ DS  L RRL++  D + 
Sbjct: 184 RTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVEV 243

Query: 246 YGDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTY 305
                R   R L  +D+RK ADIVVAKD SGKY+TI  AL+ VP+KS+KRT+IYVKKG Y
Sbjct: 244 DFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGVY 303

Query: 306 KENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINT 365
            ENV+V K  WNV++VGDG + +IVSG LNV+DGTPTF TATFA  GKGF+A DMGFINT
Sbjct: 304 FENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFINT 363

Query: 366 AGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVV 425
           AGPSKHQAVALM ++D + FYRC MNAYQDTLY H+ RQFYREC + GTVDFIFGNSA V
Sbjct: 364 AGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSASV 423

Query: 426 LQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNY 485
           LQ+C I PR P+ GQ+NTITAQGR DPN NTGISI    I P  DL    T+LGRPWKN+
Sbjct: 424 LQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKNF 483

Query: 486 STTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIK 545
           STTV M S     I   GWLPW G SAPDTIFY E++N GPG+ST  RVKWKG++ +  K
Sbjct: 484 STTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLSTK 543

Query: 546 TAKKFTVSSFIDGKDWISKAQVPFTATL 568
            A +FTV  FIDG  W+   +VPF + L
Sbjct: 544 EANRFTVKPFIDGGRWLPATKVPFRSGL 561

[Close]

BLAST of Chy12G209900.1 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 1.0e-163
Identity = 302/566 (53.36%), Postives = 398/566 (70.32%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGS- 66
           YG++DE +Q +L+ASR+T++RI +I +SS+VLV +VV  V GT    N+       NG  
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYIFDHAI 126
           +S S+KA+CDVTL+ + C +  G   N+S   P ++ K +V++ + ELSKV +     + 
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLD---GFSN 123

Query: 127 TNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQQTCID 186
               DN T  A+  C EL+ LA+D LN +++SS    LK  DDL+TW++S  TYQ+TC+D
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 187 GLAEVD-PALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQS---YG 246
            L E + P+L     N LKNSTE+TSN LAI+++  K+ D++  RR    E   +     
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 247 DWARPILRKLAQS-DIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 306
           D      R+L +S D++K+A IVVAKD SGKY+TI EAL  V +K++K T+IYVKKG Y 
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 307 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 366
           ENV V K KWNVVMVGDG + TIVS  LN +DGTPTF TATFA  GKGF+A DMGFINTA
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 367 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 426
           GP+KHQAVALM ++D S+FY+C M+A+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 427 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 486
           Q C I PR P+ GQ+NTITAQGR DPNQNTGISI    I+P D+L   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 487 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVK-NIDIK 546
           TTV M+S   + I+P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW+G+K ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 547 TAKKFTVSSFIDGKDWISKAQVPFTA 566
            A KFTV  FIDG +W+   +VPF +
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

[Close]

BLAST of Chy12G209900.1 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 9.2e-136
Identity = 271/583 (46.48%), Postives = 374/583 (64.15%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNG-- 66
           YGKV+E +Q   +  ++TR+R+++I +SS+VL+AV++A V G    + N++ S + +   
Sbjct: 10  YGKVNELEQQAYE--KKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVP 69

Query: 67  ----SLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 126
               S + S+KAVCD T YP +C  +   +  S+  DP  + KLS+++A+ ELS   + +
Sbjct: 70  QTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKL 129

Query: 127 FDHAITNTADNKTILALKNCHELLDLALDHLNISLS-----SSDITLLKAVDDLKTWITS 186
             +A     D +   A+  C  +   ALD LN S+S     +  I    +V +++TW+++
Sbjct: 130 RANA---EQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSA 189

Query: 187 AATYQQTCIDGLAEVDPALA----DLVANFLKNSTELTSNGLAIVS----FFSKLTDSLS 246
           A T Q TC+D + E++   A      +   ++NSTE  SN LAIV+      S+    + 
Sbjct: 190 ALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIH 249

Query: 247 LRRLMSYEDHQSYGDWARPILRKLAQSDIRKQA-DIVVAKDRSGKYKTITEALKAVPDKS 306
            RRL+ +       +W     R+L +        D VVAKD SG++KTI EALK V  KS
Sbjct: 250 HRRLLGFP------EWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKS 309

Query: 307 KKRTVIYVKKGTYKENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKG 366
           ++R  +YVK+G Y EN+++ KN WNV++ GDG + T V G+ N +DGTPTF TATFA KG
Sbjct: 310 EERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKG 369

Query: 367 KGFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVY 426
           KGFIA D+GF+N AG SKHQAVAL S SD+S+F+RC  + +QDTLYAHSNRQFYR+C + 
Sbjct: 370 KGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDIT 429

Query: 427 GTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLK 486
           GT+DFIFGN+AVV Q+C I PR PL  Q NTITAQG+ DPNQNTGI IQ S I PF +  
Sbjct: 430 GTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNL 489

Query: 487 TTETYLGRPWKNYSTTVFMQSNFGRLIHPTGWLPWV-GTSAPDTIFYAEFQNYGPGSSTS 546
           T  TYLGRPWK++STTV MQS+ G L++P GW+ WV     P TIFYAE+QN GPG+  S
Sbjct: 490 TAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVS 549

Query: 547 KRVKWKGVK-NIDIKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
           +RVKW G K  I  + A++FTV SFI G +W+  A V F +TL
Sbjct: 550 QRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

[Close]

BLAST of Chy12G209900.1 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 1.1e-123
Identity = 257/575 (44.70%), Postives = 360/575 (62.61%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGSL 66
           YGKVDE   L L   ++TR+R++++ +S VVL+AV++A V  T    N N+ + +    L
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  --STSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYIFDHA 126
             STS+KA+C VT +P++C  +   + +S+  DP  + KLS+++ + EL  +++ + +  
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKL 129

Query: 127 ITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLK------AVDDLKTWITSAAT 186
              T D +   AL+ C +L++ ALD LN ++S+ D    K       ++DLKTW+++  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIDGLAEV--------DPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL-- 246
             +TC D L E+        +  +   + + +  STE TSN LAIVS        L +  
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 ---RRLMSYEDHQS--YGDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVP 306
              RRLMS+   QS  +  WAR   R+L Q+   K  D+ VA D +G   T+ EA+  VP
Sbjct: 250 HRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVP 309

Query: 307 DKSKKRTVIYVKKGTYKENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFA 366
            KS K  VIYVK GTY ENV + K+KWNV++ GDG   TI+SG+ N VDGTPT+ TATFA
Sbjct: 310 KKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFA 369

Query: 367 AKGKGFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYREC 426
            +GKGFI  D+G INTAG +KHQAVA  S SD S++Y+C  + +QDTLY HSNRQFYR+C
Sbjct: 370 IQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDC 429

Query: 427 KVYGTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFD 486
            V GT+DFIFG++AVV Q C I PR PL  Q NTITAQG+ DPNQ++G+SIQ   I    
Sbjct: 430 DVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANG 489

Query: 487 DLKTTETYLGRPWKNYSTTVFMQSNFGRLIHPTGWLPWV-GTSAPDTIFYAEFQNYGPGS 546
           ++    TYLGRPWK +STTV M++  G ++ P+GW+ WV G   P +I Y E++N GPGS
Sbjct: 490 NV-IAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGS 549

Query: 547 STSKRVKWKGVKNI-DIKTAKKFTVSSFIDGKDWI 557
             ++RVKW G K +     A KFTV++ + G DWI
Sbjct: 550 DVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

[Close]

BLAST of Chy12G209900.1 vs. ExPASy Swiss-Prot
Match: Q9LUL8 (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 9.6e-109
Identity = 235/512 (45.90%), Postives = 308/512 (60.16%), Query Frame = 0


Query: 67  STSIKAVCDVTLYPDTCQKAFGPI-ANSSHLDPGQISKLSVQLALGELSKVANYIFDHAI 126
           S+ ++ VC+VT YP +C  +   +  + +  DP  + +LS+Q+   EL+ +   +     
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVG-LPKKLA 515

Query: 127 TNTADNKTILALKNCHELLDLALDHLNISLSSSDITL--------LKAVDDLKTWITSAA 186
             T D     AL  C ++ DLA+D +N ++SS D  +           + DL TW++SA 
Sbjct: 516 EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAV 575

Query: 187 TYQQTCIDGLAE--VDPALADLVANFLKNSTELTSNGLAIVSFF----SKLTDSLSLRRL 246
           T   TC D L E   +  +   + + + NSTE TSN LAIV+      SK    +  RRL
Sbjct: 576 TDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRL 635

Query: 247 MSYEDHQSYGDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTV 306
           +   +  S+ +W RP +R+L Q+       + VA D SG  +T+ EA+  VP K K   V
Sbjct: 636 L---NSNSFPNWVRPGVRRLLQAK-NLTPHVTVAADGSGDVRTVNEAVWRVPKKGKTMFV 695

Query: 307 IYVKKGTYKENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIA 366
           IYVK GTY ENV + K+KWNV + GDG + TI+SG+ N+VDG  TF+T+TFA +GKGF+ 
Sbjct: 696 IYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFMM 755

Query: 367 IDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDF 426
            DMG INTAGP KHQAVA  S SD+S++YRC  + YQDTLY HSNRQ+YR C V GTVDF
Sbjct: 756 KDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVDF 815

Query: 427 IFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETY 486
           IFG   VV Q C+I PR PL  Q NTITA+G  + NQNTGISI    I P  ++ T  TY
Sbjct: 816 IFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPNGNV-TATTY 875

Query: 487 LGRPWKNYSTTVFMQSNFGRLIHPTGWLPWVGT--SAPDTIFYAEFQNYGPGSSTSKRVK 546
           LGRPWK +S TV MQS  G  ++P GW+ W  T    P TIFY E++N GPGS  SKRVK
Sbjct: 876 LGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRVK 935

Query: 547 WKGVKNIDI-KTAKKFTVSSFIDGKD-WISKA 560
           W G K I     A +FTV  F+ G D WI KA
Sbjct: 936 WAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

[Close]

BLAST of Chy12G209900.1 vs. ExPASy TrEMBL
Match: A0A0A0LY08 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G538170 PE=3 SV=1)

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 553/567 (97.53%), Postives = 557/567 (98.24%), Query Frame = 0


Query: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MSFLRPYGKVDETDQLRLDA RRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 120
           NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVA+YI
Sbjct: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 FDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAIT TADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSY 240
           QTCID LAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYE+HQS 
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQSDIRK ADIVVAKDRSGKYKTITEAL AVPDKSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVMVGDGM ATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPF+DLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKT 540
           TTVFMQSN GRLIHPTGWLPW+GTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNID KT
Sbjct: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Chy12G209900.1 vs. ExPASy TrEMBL
Match: A0A5A7VDG1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G00770 PE=3 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 1.1e-309
Identity = 544/567 (95.94%), Postives = 556/567 (98.06%), Query Frame = 0


Query: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 120
           NQNGSLS SIKAVCDVTLYPDTCQKAFGP ANSSHLDPGQISKL+VQLALGELSKVA++I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAITNT D+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSY 240
           QTCIDGLAEVDPALADL ANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYED QS 
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQSDIRKQADIVVAKD SGKYKTITEALKAVPDKSKKRTVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           E VEVVKNKWNVVMVGDGMNATIVSG+LNV+DGTPTFSTA+FAAKGKGFIAIDMGFINTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFD+LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKT 540
           TTVFMQSN G LIHP+GWLPWVGTSAPDTIFYAEFQNYG GSSTSKRVKWKGVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Chy12G209900.1 vs. ExPASy TrEMBL
Match: A0A1S3BDA4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 1.1e-309
Identity = 544/567 (95.94%), Postives = 556/567 (98.06%), Query Frame = 0


Query: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 120
           NQNGSLS SIKAVCDVTLYPDTCQKAFGP ANSSHLDPGQISKL+VQLALGELSKVA++I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAITNT D+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSY 240
           QTCIDGLAEVDPALADL ANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYED QS 
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQSDIRKQADIVVAKD SGKYKTITEALKAVPDKSKKRTVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           E VEVVKNKWNVVMVGDGMNATIVSG+LNV+DGTPTFSTA+FAAKGKGFIAIDMGFINTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFD+LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKT 540
           TTVFMQSN G LIHP+GWLPWVGTSAPDTIFYAEFQNYG GSSTSKRVKWKGVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Chy12G209900.1 vs. ExPASy TrEMBL
Match: A0A5D3C2L2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold178G00410 PE=3 SV=1)

HSP 1 Score: 1069.7 bits (2765), Expect = 4.2e-309
Identity = 543/567 (95.77%), Postives = 555/567 (97.88%), Query Frame = 0


Query: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 120
           NQNGSLS SIKAVCDVTLYPDTCQKAFGP ANSSHLDPGQISKL+VQLALGELSKVA++I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAITNT D+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSY 240
           QTCIDGLAEVDPALADL ANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYED QS 
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQSDIRKQADIVVAKD SGKYKTITEALKAVPDKSKKRTVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           E VEVVKNKWNVVMVGDGMNATIVSG+LNV+DGTPTFSTA+FAAKGKGFIAIDMGFINTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQ NTITAQGRFDPNQNTGISIQGSLIQPFD+LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQNNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKT 540
           TTVFMQSN G LIHP+GWLPWVGTSAPDTIFYAEFQNYG GSSTSKRVKWKGVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Chy12G209900.1 vs. ExPASy TrEMBL
Match: A0A6J1KL23 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 3.1e-280
Identity = 486/567 (85.71%), Postives = 529/567 (93.30%), Query Frame = 0


Query: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MS LRPYGKVDE+DQ+RLDASRRTR+R++VIVLSSVVLV VVVAVVFGTRN S+ ND+S+
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRN-SSKNDESD 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 120
           N N +LSTSIKAVCD TLYPDTC  AFGP+ANSSHLDPGQI+K SVQLAL ELS+VA+Y+
Sbjct: 61  NHNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYL 120

Query: 121 FDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
            +HAITN+ DNKTILAL+ CHELLDLA+DHLN SLSSS+IT+LKAVDDLKTW++SAATYQ
Sbjct: 121 LEHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQ 180

Query: 181 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSY 240
           QTCIDGL EVD AL +++AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSYED  S 
Sbjct: 181 QTCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSN 240

Query: 241 GDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQ D+RKQADIVVAKD+SGKYKTITEALKAV DKSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVM+GDGM +T V+G LN +DGTPTFSTATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYR+CKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQ+TGISIQ S IQPF++LKTT+TYLGRPWKNYS
Sbjct: 421 QNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYS 480

Query: 481 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKT 540
           TTVFMQ+NFG LIHPTGWLPWVGTSAPDTIFYAEFQN GPGSST+ RVKWKG+KNID KT
Sbjct: 481 TTVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 568
           AKKFTVSSFIDGKDWISKAQVPF ATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFNATL 566

[Close]

BLAST of Chy12G209900.1 vs. NCBI nr
Match: XP_004135547.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucumis sativus] >KGN65919.1 hypothetical protein Csa_023371 [Cucumis sativus])

HSP 1 Score: 1077 bits (2786), Expect = 0.0
Identity = 553/567 (97.53%), Postives = 557/567 (98.24%), Query Frame = 0


Query: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MSFLRPYGKVDETDQLRLDA RRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 120
           NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVA+YI
Sbjct: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 FDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAIT TADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSY 240
           QTCID LAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYE+HQS 
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQSDIRK ADIVVAKDRSGKYKTITEAL AVPDKSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVMVGDGM ATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPF+DLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKT 540
           TTVFMQSN GRLIHPTGWLPW+GTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNID KT
Sbjct: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Chy12G209900.1 vs. NCBI nr
Match: XP_008445366.1 (PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Cucumis melo] >KAA0063781.1 putative pectinesterase/pectinesterase inhibitor 24 [Cucumis melo var. makuwa])

HSP 1 Score: 1064 bits (2751), Expect = 0.0
Identity = 544/567 (95.94%), Postives = 556/567 (98.06%), Query Frame = 0


Query: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 120
           NQNGSLS SIKAVCDVTLYPDTCQKAFGP ANSSHLDPGQISKL+VQLALGELSKVA++I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAITNT D+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSY 240
           QTCIDGLAEVDPALADL ANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYED QS 
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQSDIRKQADIVVAKD SGKYKTITEALKAVPDKSKKRTVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           E VEVVKNKWNVVMVGDGMNATIVSG+LNV+DGTPTFSTA+FAAKGKGFIAIDMGFINTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFD+LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKT 540
           TTVFMQSN G LIHP+GWLPWVGTSAPDTIFYAEFQNYG GSSTSKRVKWKGVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Chy12G209900.1 vs. NCBI nr
Match: TYK05534.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucumis melo var. makuwa])

HSP 1 Score: 1062 bits (2746), Expect = 0.0
Identity = 543/567 (95.77%), Postives = 555/567 (97.88%), Query Frame = 0


Query: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 120
           NQNGSLS SIKAVCDVTLYPDTCQKAFGP ANSSHLDPGQISKL+VQLALGELSKVA++I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           FDHAITNT D+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSY 240
           QTCIDGLAEVDPALADL ANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYED QS 
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQSDIRKQADIVVAKD SGKYKTITEALKAVPDKSKKRTVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           E VEVVKNKWNVVMVGDGMNATIVSG+LNV+DGTPTFSTA+FAAKGKGFIAIDMGFINTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQ NTITAQGRFDPNQNTGISIQGSLIQPFD+LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQNNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKT 540
           TTVFMQSN G LIHP+GWLPWVGTSAPDTIFYAEFQNYG GSSTSKRVKWKGVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567
           AKKFTVSSFIDGKDWISKAQVPFTATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Chy12G209900.1 vs. NCBI nr
Match: XP_038895610.1 (putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida])

HSP 1 Score: 1021 bits (2640), Expect = 0.0
Identity = 518/567 (91.36%), Postives = 546/567 (96.30%), Query Frame = 0


Query: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MS LRPYGKVDETDQLRLDASRRTR+RI VIVLSSVVLV VVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 120
           NQ+G+LSTSIKAVCDVTLYP TCQ AFGP+ANSSHLDP QISKLSVQLALGELS+VA+YI
Sbjct: 61  NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120

Query: 121 FDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
           F HAITN+ DNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSY 240
           QTCIDGL EVDPALA+L+AN+LKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQS 
Sbjct: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240

Query: 241 GDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQ DIRKQADIVVAKD SGKYKTIT AL+AVP+KSKKRTVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVV+VGDGM ATIV+G+LNV+DGTPTFSTATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GP+KHQAVALMSTSDQSIFYRC+M+AYQDTLY+HSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQ S IQP+++LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKT 540
           TTVFMQSN G LIHP+GWLPWVGTSAPDTIFYAEFQNYGPGSST+KRVKW GVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567
           AKKFTVSSFIDGKDWISKAQVPF A++
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 567

[Close]

BLAST of Chy12G209900.1 vs. NCBI nr
Match: XP_023000829.1 (probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima])

HSP 1 Score: 966 bits (2498), Expect = 0.0
Identity = 486/567 (85.71%), Postives = 529/567 (93.30%), Query Frame = 0


Query: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60
           MS LRPYGKVDE+DQ+RLDASRRTR+R++VIVLSSVVLV VVVAVVFGTRN+S N D+S+
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKN-DESD 60

Query: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYI 120
           N N +LSTSIKAVCD TLYPDTC  AFGP+ANSSHLDPGQI+K SVQLAL ELS+VA+Y+
Sbjct: 61  NHNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYL 120

Query: 121 FDHAITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180
            +HAITN+ DNKTILAL+ CHELLDLA+DHLN SLSSS+IT+LKAVDDLKTW++SAATYQ
Sbjct: 121 LEHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQ 180

Query: 181 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSY 240
           QTCIDGL EVD AL +++AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSYED  S 
Sbjct: 181 QTCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSN 240

Query: 241 GDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQ D+RKQADIVVAKD+SGKYKTITEALKAV DKSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYK 300

Query: 301 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360
           ENVEVVKNKWNVVM+GDGM +T V+G LN +DGTPTFSTATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYR+CKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQ+TGISIQ S IQPF++LKTT+TYLGRPWKNYS
Sbjct: 421 QNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYS 480

Query: 481 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIKT 540
           TTVFMQ+NFG LIHPTGWLPWVGTSAPDTIFYAEFQN GPGSST+ RVKWKG+KNID KT
Sbjct: 481 TTVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567
           AKKFTVSSFIDGKDWISKAQVPF ATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFNATL 566

[Close]

BLAST of Chy12G209900.1 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 592.4 bits (1526), Expect = 3.8e-169
Identity = 311/568 (54.75%), Postives = 398/568 (70.07%), Query Frame = 0


Query: 6   PYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGT--RNASNNNDDSNNQN 65
           PYGKVDE + +RL+A R+TR+ I +I +S V+L  +V+  VFGT     S    ++NN  
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  GSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYIFDH 125
            S+S S+KAVCDVTL+ + C +  G   N+S L+P ++ + +V++ + E+SK  N     
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAINAF--- 123

Query: 126 AITNTADNKTILALKNCHELLDLALDHLNISLSSS---DITLLKAVDDLKTWITSAATYQ 185
             ++  D K  + +  C ELLDL +D+LN +L+SS   D+T+ + VDDL+TW++SA TYQ
Sbjct: 124 -SSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTYQ 183

Query: 186 QTCIDGLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL-RRLMSYEDHQS 245
           +TC++ LA   P +     + LKNSTELTSN LAI+++  K+ DS  L RRL++  D + 
Sbjct: 184 RTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVEV 243

Query: 246 YGDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTY 305
                R   R L  +D+RK ADIVVAKD SGKY+TI  AL+ VP+KS+KRT+IYVKKG Y
Sbjct: 244 DFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGVY 303

Query: 306 KENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINT 365
            ENV+V K  WNV++VGDG + +IVSG LNV+DGTPTF TATFA  GKGF+A DMGFINT
Sbjct: 304 FENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFINT 363

Query: 366 AGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVV 425
           AGPSKHQAVALM ++D + FYRC MNAYQDTLY H+ RQFYREC + GTVDFIFGNSA V
Sbjct: 364 AGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSASV 423

Query: 426 LQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNY 485
           LQ+C I PR P+ GQ+NTITAQGR DPN NTGISI    I P  DL    T+LGRPWKN+
Sbjct: 424 LQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKNF 483

Query: 486 STTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDIK 545
           STTV M S     I   GWLPW G SAPDTIFY E++N GPG+ST  RVKWKG++ +  K
Sbjct: 484 STTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLSTK 543

Query: 546 TAKKFTVSSFIDGKDWISKAQVPFTATL 568
            A +FTV  FIDG  W+   +VPF + L
Sbjct: 544 EANRFTVKPFIDGGRWLPATKVPFRSGL 561

[Close]

BLAST of Chy12G209900.1 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 578.2 bits (1489), Expect = 7.4e-165
Identity = 302/566 (53.36%), Postives = 398/566 (70.32%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGS- 66
           YG++DE +Q +L+ASR+T++RI +I +SS+VLV +VV  V GT    N+       NG  
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYIFDHAI 126
           +S S+KA+CDVTL+ + C +  G   N+S   P ++ K +V++ + ELSKV +     + 
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLD---GFSN 123

Query: 127 TNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQQTCID 186
               DN T  A+  C EL+ LA+D LN +++SS    LK  DDL+TW++S  TYQ+TC+D
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 187 GLAEVD-PALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQS---YG 246
            L E + P+L     N LKNSTE+TSN LAI+++  K+ D++  RR    E   +     
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 247 DWARPILRKLAQS-DIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 306
           D      R+L +S D++K+A IVVAKD SGKY+TI EAL  V +K++K T+IYVKKG Y 
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 307 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 366
           ENV V K KWNVVMVGDG + TIVS  LN +DGTPTF TATFA  GKGF+A DMGFINTA
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 367 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 426
           GP+KHQAVALM ++D S+FY+C M+A+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 427 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETYLGRPWKNYS 486
           Q C I PR P+ GQ+NTITAQGR DPNQNTGISI    I+P D+L   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 487 TTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVK-NIDIK 546
           TTV M+S   + I+P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW+G+K ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 547 TAKKFTVSSFIDGKDWISKAQVPFTA 566
            A KFTV  FIDG +W+   +VPF +
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

[Close]

BLAST of Chy12G209900.1 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 445.3 bits (1144), Expect = 7.5e-125
Identity = 257/575 (44.70%), Postives = 360/575 (62.61%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGSL 66
           YGKVDE   L L   ++TR+R++++ +S VVL+AV++A V  T    N N+ + +    L
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  --STSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVANYIFDHA 126
             STS+KA+C VT +P++C  +   + +S+  DP  + KLS+++ + EL  +++ + +  
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKL 129

Query: 127 ITNTADNKTILALKNCHELLDLALDHLNISLSSSDITLLK------AVDDLKTWITSAAT 186
              T D +   AL+ C +L++ ALD LN ++S+ D    K       ++DLKTW+++  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIDGLAEV--------DPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL-- 246
             +TC D L E+        +  +   + + +  STE TSN LAIVS        L +  
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 ---RRLMSYEDHQS--YGDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVP 306
              RRLMS+   QS  +  WAR   R+L Q+   K  D+ VA D +G   T+ EA+  VP
Sbjct: 250 HRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVP 309

Query: 307 DKSKKRTVIYVKKGTYKENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFA 366
            KS K  VIYVK GTY ENV + K+KWNV++ GDG   TI+SG+ N VDGTPT+ TATFA
Sbjct: 310 KKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFA 369

Query: 367 AKGKGFIAIDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYREC 426
            +GKGFI  D+G INTAG +KHQAVA  S SD S++Y+C  + +QDTLY HSNRQFYR+C
Sbjct: 370 IQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDC 429

Query: 427 KVYGTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFD 486
            V GT+DFIFG++AVV Q C I PR PL  Q NTITAQG+ DPNQ++G+SIQ   I    
Sbjct: 430 DVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANG 489

Query: 487 DLKTTETYLGRPWKNYSTTVFMQSNFGRLIHPTGWLPWV-GTSAPDTIFYAEFQNYGPGS 546
           ++    TYLGRPWK +STTV M++  G ++ P+GW+ WV G   P +I Y E++N GPGS
Sbjct: 490 NV-IAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGS 549

Query: 547 STSKRVKWKGVKNI-DIKTAKKFTVSSFIDGKDWI 557
             ++RVKW G K +     A KFTV++ + G DWI
Sbjct: 550 DVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

[Close]

BLAST of Chy12G209900.1 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 395.6 bits (1015), Expect = 6.8e-110
Identity = 235/512 (45.90%), Postives = 308/512 (60.16%), Query Frame = 0


Query: 67  STSIKAVCDVTLYPDTCQKAFGPI-ANSSHLDPGQISKLSVQLALGELSKVANYIFDHAI 126
           S+ ++ VC+VT YP +C  +   +  + +  DP  + +LS+Q+   EL+ +   +     
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVG-LPKKLA 515

Query: 127 TNTADNKTILALKNCHELLDLALDHLNISLSSSDITL--------LKAVDDLKTWITSAA 186
             T D     AL  C ++ DLA+D +N ++SS D  +           + DL TW++SA 
Sbjct: 516 EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAV 575

Query: 187 TYQQTCIDGLAE--VDPALADLVANFLKNSTELTSNGLAIVSFF----SKLTDSLSLRRL 246
           T   TC D L E   +  +   + + + NSTE TSN LAIV+      SK    +  RRL
Sbjct: 576 TDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRL 635

Query: 247 MSYEDHQSYGDWARPILRKLAQSDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTV 306
           +   +  S+ +W RP +R+L Q+       + VA D SG  +T+ EA+  VP K K   V
Sbjct: 636 L---NSNSFPNWVRPGVRRLLQAK-NLTPHVTVAADGSGDVRTVNEAVWRVPKKGKTMFV 695

Query: 307 IYVKKGTYKENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIA 366
           IYVK GTY ENV + K+KWNV + GDG + TI+SG+ N+VDG  TF+T+TFA +GKGF+ 
Sbjct: 696 IYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFMM 755

Query: 367 IDMGFINTAGPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDF 426
            DMG INTAGP KHQAVA  S SD+S++YRC  + YQDTLY HSNRQ+YR C V GTVDF
Sbjct: 756 KDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVDF 815

Query: 427 IFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDLKTTETY 486
           IFG   VV Q C+I PR PL  Q NTITA+G  + NQNTGISI    I P  ++ T  TY
Sbjct: 816 IFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPNGNV-TATTY 875

Query: 487 LGRPWKNYSTTVFMQSNFGRLIHPTGWLPWVGT--SAPDTIFYAEFQNYGPGSSTSKRVK 546
           LGRPWK +S TV MQS  G  ++P GW+ W  T    P TIFY E++N GPGS  SKRVK
Sbjct: 876 LGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRVK 935

Query: 547 WKGVKNIDI-KTAKKFTVSSFIDGKD-WISKA 560
           W G K I     A +FTV  F+ G D WI KA
Sbjct: 936 WAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

[Close]

BLAST of Chy12G209900.1 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 386.0 bits (990), Expect = 5.4e-107
Identity = 230/571 (40.28%), Postives = 333/571 (58.32%), Query Frame = 0


Query: 19  DASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSNNQNGSLSTSIKAVCDV-- 78
           D   + +RR +VI +SSV+L+++VVAV  G   + N +D  +     ++ S+KAV DV  
Sbjct: 6   DDESKRKRRYIVITISSVLLISMVVAVTVGV--SLNKHDGDSKGKAEVNASVKAVKDVCA 65

Query: 79  -TLYPDTCQKAFGPIANSSH-LDPGQISKLSVQLALGELSKVANYIFDHAITNTADNKTI 138
            T Y  TC+     I N  +  DP ++ K +  + + +++  A       +    D++T 
Sbjct: 66  PTDYRKTCEDTL--IKNGKNTTDPMELVKTAFNVTMKQITDAAKK-SQTIMELQKDSRTR 125

Query: 139 LALKNCHELLDLALDHLNIS---LSSSDITLL-KAVDDLKTWITSAATYQQTCIDGLAEV 198
           +AL  C EL+D ALD L+ S   L   +  LL +A+ +L+ W+++A ++++TC++G    
Sbjct: 126 MALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGT 185

Query: 199 DPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSL-----RRLMSYEDHQSYGDWAR 258
                + +   LK + ELT NGLAI+S  S     + +     RRL++    + +  W  
Sbjct: 186 QGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA----EGFPSWVD 245

Query: 259 PILRKLAQ-----SDIRKQADIVVAKDRSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 318
              RKL Q     SD++   DIVVA+D SG+YKTI EAL+ VP K     V+++K G YK
Sbjct: 246 QRGRKLLQAAAAYSDVK--PDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYK 305

Query: 319 ENVEVVKNKWNVVMVGDGMNATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 378
           E V+V K   ++V +GDG + TI+SGN N  DG  T+ TAT A  G  FIA ++GF NTA
Sbjct: 306 EYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTA 365

Query: 379 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 438
           G  KHQAVA+   SD+SIF+ C  + YQDTLY HS+RQF+R+C + GT+DF+FG++A V 
Sbjct: 366 GAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVF 425

Query: 439 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDDL----KTTETYLGRPW 498
           QNCT+  R PL  Q   ITA GR DP ++TG   QG  I    D     +T++ YLGRPW
Sbjct: 426 QNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPW 485

Query: 499 KNYSTTVFMQSNFGRLIHPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNI 558
           K YS T+ M +     + P GW PW+G     T+FY+E QN GPGS+ + RV W G+K +
Sbjct: 486 KEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTL 545

Query: 559 DIKTAKKFTVSSFIDGKDWISKAQVPFTATL 568
             +   KFT + +I G DWI    VP+T  L
Sbjct: 546 SEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

The following BLAST results are available for this feature:

BLAST of Chy12G209900.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SG77	5.3e-168	54.75	Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF78	1.0e-163	53.36	Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3...	[more]
Q43111	9.2e-136	46.48	Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1	[more]
Q43867	1.1e-123	44.70	Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1	[more]
Q9LUL8	9.6e-109	45.90	Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Chy12G209900.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LY08	0.0e+00	97.53	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G538170 PE=3 SV=1	[more]
A0A5A7VDG1	1.1e-309	95.94	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G0077...	[more]
A0A1S3BDA4	1.1e-309	95.94	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1	[more]
A0A5D3C2L2	4.2e-309	95.77	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold178G00410...	[more]
A0A6J1KL23	3.1e-280	85.71	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1	[more]

BLAST of Chy12G209900.1 vs. NCBI nr


Match Name	Identity	Description
XP_004135547.1	97.53	putative pectinesterase/pectinesterase inhibitor 24 [Cucumis sativus] >KGN65919....	[more]
XP_008445366.1	95.94	PREDICTED: putative pectinesterase/pectinesterase inhibitor 24 [Cucumis melo] >K...	[more]
TYK05534.1	95.77	putative pectinesterase/pectinesterase inhibitor 24 [Cucumis melo var. makuwa]	[more]
XP_038895610.1	91.36	putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida]	[more]
XP_023000829.1	85.71	probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima]	[more]

BLAST of Chy12G209900.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10710.1	3.8e-169	54.75	root hair specific 12	[more]
AT5G04960.1	7.4e-165	53.36	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G53840.1	7.5e-125	44.70	pectin methylesterase 1	[more]
AT3G14300.1	6.8e-110	45.90	pectinesterase family protein	[more]
AT3G05610.1	5.4e-107	40.28	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 65..215 e-value: 6.8E-28 score: 108.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 69..215 e-value: 1.8E-24 score: 86.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 41..216 e-value: 1.2E-20 score: 71.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 256..567 e-value: 6.0E-113 score: 379.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 67..219 e-value: 1.3E-29 score: 105.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 69..220
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 261..552 e-value: 4.4E-139 score: 462.3
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 19..563
None	No IPR available	PANTHER	PTHR31707:SF272	PECTINESTERASE/PECTINESTERASE INHIBITOR 24-RELATED	coord: 19..563
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 72..221 e-value: 1.64872E-35 score: 128.71
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 286..305
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 405..414
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 259..566

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Chy12G209900	Chy12G209900	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy12G209900.1.exon2	Chy12G209900.1.exon2	exon
Chy12G209900.1.exon1	Chy12G209900.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Chy12G209900.1.cds2	Chy12G209900.1.cds2	CDS
Chy12G209900.1.cds1	Chy12G209900.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Chy12G209900.1	Chy12G209900.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity