CcUC08G149690.1 (mRNA) Watermelon (PI 537277) v1

Overview
NameCcUC08G149690.1
TypemRNA
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionEukaryotic aspartyl protease family protein
LocationCicolChr08: 17477806 .. 17479101 (+)
Sequence length1296
RNA-Seq ExpressionCcUC08G149690.1
SyntenyCcUC08G149690.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAATTGTTGCTTCTTGTTACTGCTTTTGTTGCCAGTTCTCCATTCCCAGCCAGCTTTCAGTGAAAGTTCACTAAAACCTAGATTTCATTTTGATCTTACACATGTTGATGCTACCCAAAACTTCACCAAATTCGAAATGTTCCGACGAGGAATTGAGCGAGACAAGACCCGGATACAAAATTTCAAAAAGATGACTGTAAACTTCGAAGTTCGAATGCCACTCGTTGACCAACAAGGGAGCTATCTGATGAACTTGTCATTCGGAACGCCTCCTGTTTCGTTCTCTGCAATTTTCGATACTGGCAGTGACTTGATTTGGACACAGTGCAATCCATGCTTGAAATGTTTTGGGCAACCAACCCCCGTGTATGATCCTGCACAATCGTCTTCCTTCACCAATGCTACTCGCTCCACTGCATTATGCCAGGCTTTGACCAATTCAAGTTCCAGCAATGGTTGTGAGTACTCATATGGTTATGGAGATGGATCTTTCACCATAGGTTACTTGGCGTTCGAGACCTTGACGCTTGGTGAAGCAAACCAGCAAGTTTCTACACCGGACATAGCTTTCGGGTGCGGGATAAGGAACTACGTAAAGGGCTTGACGCAGGGTGCTGGCATAGTAGGGTTCAGCAGAGGACCATTGTCGCTTCTTTCGCAGCTCCATATTCGAAAGTTCTCTTACTGTTTAAGTCCAAATGGAACTGGGTGTCTGGCCTTTGGATCATCTTCAACAAGCTTTGACAACACAACAAACCTAGCCGTCAAGAACACGCCACTGATACAAAATCCATCCAATCCATCTTACTACTATCTATCTCTACAAGGAATCACAGTTGGCCAAAAGTTTTTGCCCGTCCCATCCTCGTGGTTTGAACTAAATGCTGATGGCAGCGGAGGCATGATCATAGATTCTGGCACATCAATCACATATATAACAGAGGATGCCTTTGATGTGCTGAAGCCAGTTTTTACTTCACAGACAAACCTCCCGGTGATAAATTCAGCAAGTATTGGTCTTGACCTCTGCTTTGAGCTGCCTTCCCCAAACAATAGTAAACTTGATGTGCCTGATTTGATATTTCATTTTGAGGGTCTCGACTTGAAGCTTCCAGTCGAGAACTATATGGTCGTCAATGAGGAGGTAGGGATTGTATGCTTGGCAATGGGGGCTGCAGGAGGTTTGTCGATTTTTGGCAGCATGCAACATCAAAATATGTTGGTTCTTCATGATCTAAAGAAAAAAGTCATATCATTCATTCCCACACAATGCTCTCGGATCAATTACTAG

mRNA sequence

ATGGCAAATTGTTGCTTCTTGTTACTGCTTTTGTTGCCAGTTCTCCATTCCCAGCCAGCTTTCAGTGAAAGTTCACTAAAACCTAGATTTCATTTTGATCTTACACATGTTGATGCTACCCAAAACTTCACCAAATTCGAAATGTTCCGACGAGGAATTGAGCGAGACAAGACCCGGATACAAAATTTCAAAAAGATGACTGTAAACTTCGAAGTTCGAATGCCACTCGTTGACCAACAAGGGAGCTATCTGATGAACTTGTCATTCGGAACGCCTCCTGTTTCGTTCTCTGCAATTTTCGATACTGGCAGTGACTTGATTTGGACACAGTGCAATCCATGCTTGAAATGTTTTGGGCAACCAACCCCCGTGTATGATCCTGCACAATCGTCTTCCTTCACCAATGCTACTCGCTCCACTGCATTATGCCAGGCTTTGACCAATTCAAGTTCCAGCAATGGTTGTGAGTACTCATATGGTTATGGAGATGGATCTTTCACCATAGGTTACTTGGCGTTCGAGACCTTGACGCTTGGTGAAGCAAACCAGCAAGTTTCTACACCGGACATAGCTTTCGGGTGCGGGATAAGGAACTACGTAAAGGGCTTGACGCAGGGTGCTGGCATAGTAGGGTTCAGCAGAGGACCATTGTCGCTTCTTTCGCAGCTCCATATTCGAAAGTTCTCTTACTGTTTAAGTCCAAATGGAACTGGGTGTCTGGCCTTTGGATCATCTTCAACAAGCTTTGACAACACAACAAACCTAGCCGTCAAGAACACGCCACTGATACAAAATCCATCCAATCCATCTTACTACTATCTATCTCTACAAGGAATCACAGTTGGCCAAAAGTTTTTGCCCGTCCCATCCTCGTGGTTTGAACTAAATGCTGATGGCAGCGGAGGCATGATCATAGATTCTGGCACATCAATCACATATATAACAGAGGATGCCTTTGATGTGCTGAAGCCAGTTTTTACTTCACAGACAAACCTCCCGGTGATAAATTCAGCAAGTATTGGTCTTGACCTCTGCTTTGAGCTGCCTTCCCCAAACAATAGTAAACTTGATGTGCCTGATTTGATATTTCATTTTGAGGGTCTCGACTTGAAGCTTCCAGTCGAGAACTATATGGTCGTCAATGAGGAGGTAGGGATTGTATGCTTGGCAATGGGGGCTGCAGGAGGTTTGTCGATTTTTGGCAGCATGCAACATCAAAATATGTTGGTTCTTCATGATCTAAAGAAAAAAGTCATATCATTCATTCCCACACAATGCTCTCGGATCAATTACTAG

Coding sequence (CDS)

ATGGCAAATTGTTGCTTCTTGTTACTGCTTTTGTTGCCAGTTCTCCATTCCCAGCCAGCTTTCAGTGAAAGTTCACTAAAACCTAGATTTCATTTTGATCTTACACATGTTGATGCTACCCAAAACTTCACCAAATTCGAAATGTTCCGACGAGGAATTGAGCGAGACAAGACCCGGATACAAAATTTCAAAAAGATGACTGTAAACTTCGAAGTTCGAATGCCACTCGTTGACCAACAAGGGAGCTATCTGATGAACTTGTCATTCGGAACGCCTCCTGTTTCGTTCTCTGCAATTTTCGATACTGGCAGTGACTTGATTTGGACACAGTGCAATCCATGCTTGAAATGTTTTGGGCAACCAACCCCCGTGTATGATCCTGCACAATCGTCTTCCTTCACCAATGCTACTCGCTCCACTGCATTATGCCAGGCTTTGACCAATTCAAGTTCCAGCAATGGTTGTGAGTACTCATATGGTTATGGAGATGGATCTTTCACCATAGGTTACTTGGCGTTCGAGACCTTGACGCTTGGTGAAGCAAACCAGCAAGTTTCTACACCGGACATAGCTTTCGGGTGCGGGATAAGGAACTACGTAAAGGGCTTGACGCAGGGTGCTGGCATAGTAGGGTTCAGCAGAGGACCATTGTCGCTTCTTTCGCAGCTCCATATTCGAAAGTTCTCTTACTGTTTAAGTCCAAATGGAACTGGGTGTCTGGCCTTTGGATCATCTTCAACAAGCTTTGACAACACAACAAACCTAGCCGTCAAGAACACGCCACTGATACAAAATCCATCCAATCCATCTTACTACTATCTATCTCTACAAGGAATCACAGTTGGCCAAAAGTTTTTGCCCGTCCCATCCTCGTGGTTTGAACTAAATGCTGATGGCAGCGGAGGCATGATCATAGATTCTGGCACATCAATCACATATATAACAGAGGATGCCTTTGATGTGCTGAAGCCAGTTTTTACTTCACAGACAAACCTCCCGGTGATAAATTCAGCAAGTATTGGTCTTGACCTCTGCTTTGAGCTGCCTTCCCCAAACAATAGTAAACTTGATGTGCCTGATTTGATATTTCATTTTGAGGGTCTCGACTTGAAGCTTCCAGTCGAGAACTATATGGTCGTCAATGAGGAGGTAGGGATTGTATGCTTGGCAATGGGGGCTGCAGGAGGTTTGTCGATTTTTGGCAGCATGCAACATCAAAATATGTTGGTTCTTCATGATCTAAAGAAAAAAGTCATATCATTCATTCCCACACAATGCTCTCGGATCAATTACTAG

Protein sequence

MANCCFLLLLLLPVLHSQPAFSESSLKPRFHFDLTHVDATQNFTKFEMFRRGIERDKTRIQNFKKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLKCFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFETLTLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLSPNGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSSWFELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSPNNSKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVLHDLKKKVISFIPTQCSRINY
Homology
BLAST of CcUC08G149690.1 vs. NCBI nr
Match: XP_038886455.1 (aspartic proteinase nepenthesin-2-like [Benincasa hispida])

HSP 1 Score: 725.7 bits (1872), Expect = 2.3e-205
Identity = 363/432 (84.03%), Postives = 393/432 (90.97%), Query Frame = 0

Query: 1   MANCCFLLLLLLPVLHSQPAFSESSLKPRFHFDLTHVDATQNFTKFEMFRRGIERDKTRI 60
           MAN CFLLLLLLP+L S+PAFSESSLKP FHFDLTHVDAT NF+KFE+ RRGI+RDKT I
Sbjct: 3   MANFCFLLLLLLPLLSSKPAFSESSLKPGFHFDLTHVDATWNFSKFEILRRGIKRDKTWI 62

Query: 61  QNFKKMTV-NFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLKCFG 120
           QNF KM + N +VRMP+V+Q+GSYLMNLS GTPP+SFSAIFDTGSDL+WTQC PCL+CF 
Sbjct: 63  QNFNKMIISNLKVRMPVVEQRGSYLMNLSLGTPPISFSAIFDTGSDLVWTQCKPCLQCFK 122

Query: 121 QPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFETLTLG 180
           QPTPVYDPAQSSSF NATRS ALC+ LTNSSSSNGCEYS+ Y D SFT GYLAFETLTLG
Sbjct: 123 QPTPVYDPAQSSSFANATRSAALCKELTNSSSSNGCEYSFSYADESFTTGYLAFETLTLG 182

Query: 181 EANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLSPNGTGC 240
            ANQ+VSTPDIAFGCGI+N+VKGLTQGAGIVGF+RGPLSLLSQLHI+KFSYCLS NGTG 
Sbjct: 183 GANQKVSTPDIAFGCGIKNHVKGLTQGAGIVGFNRGPLSLLSQLHIKKFSYCLSLNGTGS 242

Query: 241 LAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSSWFELNADG 300
           L FG SSTS DNTTN A+K TPLIQNP NPSYYYLSLQGITVGQK+LPVPSS F LN DG
Sbjct: 243 LVFG-SSTSIDNTTNRAIKTTPLIQNPYNPSYYYLSLQGITVGQKYLPVPSSSFRLNTDG 302

Query: 301 SGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSPNNSKLDVP 360
           SGGMIIDSGTS+TYITED FDVLKPVF SQTNL VI S SIGLDLCF+LPS NNSKLDVP
Sbjct: 303 SGGMIIDSGTSMTYITEDVFDVLKPVFASQTNLQVIWS-SIGLDLCFQLPSHNNSKLDVP 362

Query: 361 DLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVLHDLKKKVI 420
           DLIFHFE LDLKLPVENYMVVNEE+GIVCLAMGAAGGLSIFGSMQHQNMLVLHDL+KKV+
Sbjct: 363 DLIFHFEDLDLKLPVENYMVVNEELGIVCLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVV 422

Query: 421 SFIPTQCSRINY 432
           SFIPTQCS+++Y
Sbjct: 423 SFIPTQCSQLDY 432

BLAST of CcUC08G149690.1 vs. NCBI nr
Match: RVX22508.1 (Aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 446.0 bits (1146), Expect = 3.6e-121
Identity = 231/436 (52.98%), Postives = 294/436 (67.43%), Query Frame = 0

Query: 6   FLLLLL----LPVLHS--QPAFSESSLKPRFHFDLTHVDATQNFTKFEMFRRGIERDKTR 65
           FLLL+L    LP   S   P F    L+  F   L H+DA +NFTK ++ +RGI R + R
Sbjct: 13  FLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTKLQLIQRGINRGRQR 72

Query: 66  IQNFKKMTVNFE---VRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLK 125
           +Q    M    E    + P+    G +++NL  GTPPV F AI DTGSDLIWTQCNPC  
Sbjct: 73  LQRMSGMATTAERNDFQPPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLIWTQCNPCKL 132

Query: 126 CFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFETL 185
           CF Q TPV++P +SS+F+N + S+ LC+ +  S     CEY Y YGD S T G++A +T+
Sbjct: 133 CFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESSTEGFMAMDTI 192

Query: 186 TLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLS--- 245
           T GE+ ++VS P I FGCG+ N   G+ Q AG++G  RG LSL+SQL  +KFSYCL+   
Sbjct: 193 TFGESPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQKFSYCLTSIH 252

Query: 246 PNGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSSWF 305
            N T  L FG  S ++ N     +  TPLIQNP  PSYYYLSL+GITVG   LP+P   F
Sbjct: 253 ENKTSSLLFG--SLAYSNFNPGKIPRTPLIQNPFLPSYYYLSLKGITVGYTLLPIPEFAF 312

Query: 306 ELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSPNN 365
           +L  DGSGGMI+DSGT+ITY+ EDAFDVLK  F SQT L V NS++ GLDLCF LP  N 
Sbjct: 313 QLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDLCFHLPVKNA 372

Query: 366 SKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVLHD 425
           +++ VP LIFHF+GLDL LPVENYMV + E+G++CLA+ A G LSIFG++Q QNMLVLHD
Sbjct: 373 AEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQQQNMLVLHD 432

Query: 426 LKKKVISFIPTQCSRI 430
           LKK  +S +PTQC ++
Sbjct: 433 LKKSTLSLVPTQCDKV 446

BLAST of CcUC08G149690.1 vs. NCBI nr
Match: XP_034676400.1 (aspartic proteinase nepenthesin-1-like [Vitis riparia])

HSP 1 Score: 443.7 bits (1140), Expect = 1.8e-120
Identity = 229/436 (52.52%), Postives = 293/436 (67.20%), Query Frame = 0

Query: 6   FLLLLL----LPVLHS--QPAFSESSLKPRFHFDLTHVDATQNFTKFEMFRRGIERDKTR 65
           FLLL+L    LP   S   P F    L+  F   L H+DA +NFTK ++ +RGI+R + R
Sbjct: 13  FLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTKLQLIQRGIDRGRQR 72

Query: 66  IQNFKKMTVNFE---VRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLK 125
           +Q    M    E    + P+    G +++NL  GTPPV F AI DTGSDLIWTQCNPC  
Sbjct: 73  LQRMSGMATTAERNDFQAPVHAGDGEFVVNLMIGTPPVPFPAIMDTGSDLIWTQCNPCKL 132

Query: 126 CFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFETL 185
           CF Q TPV++P +SS+F+N + S+ LC+ +  S     CEY Y YGD S T G++A +T+
Sbjct: 133 CFQQSTPVFNPKRSSTFSNISCSSKLCEGVKPSKCDKSCEYRYTYGDESSTEGFMAMDTI 192

Query: 186 TLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLS--- 245
           T GE  ++VS P I FGCG+ N   G+ Q AG++G  RG LSL+SQL  +KFSYCL+   
Sbjct: 193 TFGELPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLSTQKFSYCLTSIH 252

Query: 246 PNGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSSWF 305
            N T  L FG  S ++ N     +  TPLIQNP  PSYYYLSL+GITVG   LP+P   F
Sbjct: 253 ENKTSSLLFG--SLAYSNFNPGKIPRTPLIQNPFLPSYYYLSLKGITVGYTLLPIPDFAF 312

Query: 306 ELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSPNN 365
           +L  DGSGGMI+DSGT+ITY+ EDAFDVLK  F SQT L V NS++ GLDLCF LP  N 
Sbjct: 313 QLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDLCFHLPVKNA 372

Query: 366 SKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVLHD 425
           +++ VP LIFHF+GLDL LPVENYMV + ++G++CLA+ A G  SIFG++Q QNMLVLHD
Sbjct: 373 AEVKVPKLIFHFKGLDLALPVENYMVSDPDMGLICLAIDATGSPSIFGNIQQQNMLVLHD 432

Query: 426 LKKKVISFIPTQCSRI 430
           LKK  +S +PTQC ++
Sbjct: 433 LKKSTLSLVPTQCDKV 446

BLAST of CcUC08G149690.1 vs. NCBI nr
Match: XP_010664223.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 443.4 bits (1139), Expect = 2.3e-120
Identity = 229/436 (52.52%), Postives = 293/436 (67.20%), Query Frame = 0

Query: 6   FLLLLL----LPVLHS--QPAFSESSLKPRFHFDLTHVDATQNFTKFEMFRRGIERDKTR 65
           FLLL+L    LP   S   P F    L+  F   L H+DA +NFT+ ++ +RGI R + R
Sbjct: 13  FLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTRLQLIQRGINRGRQR 72

Query: 66  IQNFKKMTVNFE---VRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLK 125
           +Q    M    E    + P+    G +++NL  GTPPV F AI DTGSDLIWTQCNPC  
Sbjct: 73  LQRMSGMATTAERNGFQAPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLIWTQCNPCKL 132

Query: 126 CFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFETL 185
           CF Q TPV++P +SS+F+N + S+ LC+ +  S     CEY Y YGD S T G++A +T+
Sbjct: 133 CFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESSTEGFMAMDTI 192

Query: 186 TLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLS--- 245
           T GE  ++VS P I FGCG+ N   G+ Q AG++G  RG LSL+SQL  +KFSYCL+   
Sbjct: 193 TFGELPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQKFSYCLTSIH 252

Query: 246 PNGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSSWF 305
            N T  L FG  S ++ N     +  TPLIQNP  PSYYYL+L+GITVG   LP+P   F
Sbjct: 253 ENKTSSLLFG--SLAYSNFNPGKIPRTPLIQNPFLPSYYYLALKGITVGYTLLPIPEFAF 312

Query: 306 ELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSPNN 365
           +L  DGSGGMI+DSGT+ITY+ EDAFDVLK  F SQT L V NS++ GLDLCF LP  N 
Sbjct: 313 QLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDLCFHLPVKNA 372

Query: 366 SKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVLHD 425
           +++ VP LIFHF+GLDL LPVENYMV + E+G++CLA+ A G LSIFG++Q QNMLVLHD
Sbjct: 373 AEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQQQNMLVLHD 432

Query: 426 LKKKVISFIPTQCSRI 430
           LKK  +S +PTQC ++
Sbjct: 433 LKKSTLSLVPTQCDKV 446

BLAST of CcUC08G149690.1 vs. NCBI nr
Match: XP_011045692.1 (PREDICTED: aspartic proteinase nepenthesin-1-like [Populus euphratica])

HSP 1 Score: 442.2 bits (1136), Expect = 5.2e-120
Identity = 227/440 (51.59%), Postives = 304/440 (69.09%), Query Frame = 0

Query: 1   MANCCFLLLLLLPVLHSQPAFSES-------SLKPRFHFDLTHVDATQNFTKFEMFRRGI 60
           M + CF+L L +  +   PAFS S        ++  F   L HVD+ +N TK E  R G+
Sbjct: 4   MTSLCFVLALAMFTIFFSPAFSTSRRALEHPEMQKGFRVRLKHVDSGKNLTKLERIRHGV 63

Query: 61  ERDKTRIQNFKKMTV----NFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWT 120
           +R + R+Q  K M +    + E+  P++   G +LM L+ GTPP ++SAI DTGSDLIWT
Sbjct: 64  KRGRNRLQRLKTMALVASSSSEIEAPVLPGNGEFLMKLAIGTPPETYSAILDTGSDLIWT 123

Query: 121 QCNPCLKCFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTIG 180
           QC PC +CF Q TP++DP +SSSF+  + S+ LC+AL  SS SNGCEY Y YGD S T G
Sbjct: 124 QCKPCTQCFHQSTPIFDPKKSSSFSKLSCSSQLCEALPQSSCSNGCEYLYSYGDYSSTQG 183

Query: 181 YLAFETLTLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFS 240
            LA ETLT G+A    S P++AFGCG  N   G +QGAG+VG  RGPLSL+SQL   KFS
Sbjct: 184 ILASETLTFGKA----SVPNVAFGCGANNEGSGFSQGAGLVGLGRGPLSLVSQLKEPKFS 243

Query: 241 YCLS---PNGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFL 300
           YCL+      T  L  GS ++   N ++ A+K TPLI++P++PS+YYLSL+GI+VG   L
Sbjct: 244 YCLTSVDDAKTSTLLMGSLASV--NASSSAIKTTPLIRSPAHPSFYYLSLEGISVGDTRL 303

Query: 301 PVPSSWFELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCF 360
           P+  S F L  DGSGG+IIDSGT+ITY+ E AF+++   FTSQ NLPV +S S GLD+CF
Sbjct: 304 PIKKSTFSLQDDGSGGLIIDSGTTITYLEERAFNLVAKEFTSQINLPVDSSGSTGLDVCF 363

Query: 361 ELPSPNNSKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQ 420
            LPS  ++ ++VP L+FHF+G DL+LP ENYM+ +  +G+ CLAMG++ G+SIFG++Q Q
Sbjct: 364 TLPS-GSTNIEVPKLVFHFDGADLELPAENYMIGDSSMGVACLAMGSSSGMSIFGNVQQQ 423

Query: 421 NMLVLHDLKKKVISFIPTQC 427
           NMLVLHDL+K+ +SF+PTQC
Sbjct: 424 NMLVLHDLEKETLSFLPTQC 436

BLAST of CcUC08G149690.1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 3.4e-98
Identity = 204/437 (46.68%), Postives = 272/437 (62.24%), Query Frame = 0

Query: 6   FLLLLLLPVLHSQPAFSES--SLKPR-------FHFDLTHVDATQNFTKFEMFRRGIERD 65
           FLL L +  +   P  S S  +L  R       F   L HVD+ +N TKF++  R IER 
Sbjct: 8   FLLALSIVYIFVAPTHSTSRTALNHRHEAKVTGFQIMLEHVDSGKNLTKFQLLERAIERG 67

Query: 66  KTRIQNFKKMTVN-FEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCL 125
             R+Q  + M      V   +    G YLMNLS GTP   FSAI DTGSDLIWTQC PC 
Sbjct: 68  SRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCT 127

Query: 126 KCFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNG-CEYSYGYGDGSFTIGYLAFE 185
           +CF Q TP+++P  SSSF+    S+ LCQAL++ + SN  C+Y+YGYGDGS T G +  E
Sbjct: 128 QCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTE 187

Query: 186 TLTLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLSP 245
           TLT G     VS P+I FGCG  N   G   GAG+VG  RGPLSL SQL + KFSYC++P
Sbjct: 188 TLTFG----SVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTP 247

Query: 246 NGTGC---LAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSS 305
            G+     L  GS +    N+      NT LIQ+   P++YY++L G++VG   LP+  S
Sbjct: 248 IGSSTPSNLLLGSLA----NSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPS 307

Query: 306 WFELNA-DGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPS 365
            F LN+ +G+GG+IIDSGT++TY   +A+  ++  F SQ NLPV+N +S G DLCF+ PS
Sbjct: 308 AFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPS 367

Query: 366 PNNSKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAG-GLSIFGSMQHQNML 425
            + S L +P  + HF+G DL+LP ENY  ++   G++CLAMG++  G+SIFG++Q QNML
Sbjct: 368 -DPSNLQIPTFVMHFDGGDLELPSENYF-ISPSNGLICLAMGSSSQGMSIFGNIQQQNML 427

Query: 426 VLHDLKKKVISFIPTQC 427
           V++D    V+SF   QC
Sbjct: 428 VVYDTGNSVVSFASAQC 434

BLAST of CcUC08G149690.1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 2.7e-95
Identity = 184/407 (45.21%), Postives = 264/407 (64.86%), Query Frame = 0

Query: 27  KPRFHFDLTHVDATQNFTKFEMFRRGIERDKTRIQNFKKMTVNFE-VRMPLVDQQGSYLM 86
           +P    DL  VD+ +N TK+E+ +R I+R + R+++   M  +   +  P+    G YLM
Sbjct: 39  QPGLRVDLEQVDSGKNLTKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLM 98

Query: 87  NLSFGTPPVSFSAIFDTGSDLIWTQCNPCLKCFGQPTPVYDPAQSSSFTNATRSTALCQA 146
           N++ GTP  SFSAI DTGSDLIWTQC PC +CF QPTP+++P  SSSF+     +  CQ 
Sbjct: 99  NVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQD 158

Query: 147 L-TNSSSSNGCEYSYGYGDGSFTIGYLAFETLTLGEANQQVSTPDIAFGCGIRNYVKGLT 206
           L + + ++N C+Y+YGYGDGS T GY+A ET T     +  S P+IAFGCG  N   G  
Sbjct: 159 LPSETCNNNECQYTYGYGDGSTTQGYMATETFTF----ETSSVPNIAFGCGEDNQGFGQG 218

Query: 207 QGAGIVGFSRGPLSLLSQLHIRKFSYCLSPNGT---GCLAFGSSSTSFDNTTNLAVKNTP 266
            GAG++G   GPLSL SQL + +FSYC++  G+     LA GS+++     +     +T 
Sbjct: 219 NGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGS----PSTT 278

Query: 267 LIQNPSNPSYYYLSLQGITVGQKFLPVPSSWFELNADGSGGMIIDSGTSITYITEDAFDV 326
           LI +  NP+YYY++LQGITVG   L +PSS F+L  DG+GGMIIDSGT++TY+ +DA++ 
Sbjct: 279 LIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNA 338

Query: 327 LKPVFTSQTNLPVINSASIGLDLCFELPSPNNSKLDVPDLIFHFEGLDLKLPVENYMVVN 386
           +   FT Q NLP ++ +S GL  CF+ PS + S + VP++   F+G  L L  +N ++  
Sbjct: 339 VAQAFTDQINLPTVDESSSGLSTCFQQPS-DGSTVQVPEISMQFDGGVLNLGEQNILISP 398

Query: 387 EEVGIVCLAMGAAG--GLSIFGSMQHQNMLVLHDLKKKVISFIPTQC 427
            E G++CLAMG++   G+SIFG++Q Q   VL+DL+   +SF+PTQC
Sbjct: 399 AE-GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of CcUC08G149690.1 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.3e-62
Identity = 157/445 (35.28%), Postives = 231/445 (51.91%), Query Frame = 0

Query: 6   FLLLLLLPVLHSQPAFSESSLKPRFHF--DLTHVDATQN------FTKFEMFRRGIERDK 65
           F  +LL   L S    S ++ KP+  F  DL H D+ ++       T  +  R  I R  
Sbjct: 5   FSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSV 64

Query: 66  TRIQNFKKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLKC 125
            R+ +F +     + ++ L    G YLMN+S GTPP    AI DTGSDL+WTQC PC  C
Sbjct: 65  NRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDC 124

Query: 126 FGQPTPVYDPAQSSSFTNATRSTALCQALTN----SSSSNGCEYSYGYGDGSFTIGYLAF 185
           + Q  P++DP  SS++ + + S++ C AL N    S++ N C YS  YGD S+T G +A 
Sbjct: 125 YTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAV 184

Query: 186 ETLTLGEAN-QQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIR---KFS 245
           +TLTLG ++ + +   +I  GCG  N      +G+GIVG   GP+SL+ QL      KFS
Sbjct: 185 DTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFS 244

Query: 246 YCLSP-----NGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQK 305
           YCL P     + T  + FG+++      +   V +TPLI   S  ++YYL+L+ I+VG K
Sbjct: 245 YCLVPLTSKKDQTSKINFGTNAI----VSGSGVVSTPLIAKASQETFYYLTLKSISVGSK 304

Query: 306 FLPVPSSWFELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDL 365
            +    S  E      G +IIDSGT++T +  + +  L+    S  +         GL L
Sbjct: 305 QIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSL 364

Query: 366 CFELPSPNNSKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQ 425
           C+         L VP +  HF+G D+KL   N  V   E  +VC A   +   SI+G++ 
Sbjct: 365 CYSA----TGDLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNVA 424

Query: 426 HQNMLVLHDLKKKVISFIPTQCSRI 430
             N LV +D   K +SF PT C+++
Sbjct: 425 QMNFLVGYDTVSKTVSFKPTDCAKM 437

BLAST of CcUC08G149690.1 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 226.5 bits (576), Expect = 5.8e-58
Identity = 158/471 (33.55%), Postives = 233/471 (49.47%), Query Frame = 0

Query: 8   LLLLLPVLHSQPAFSESSLKPR-FHFDLTHVDAT----QNFTKFEMFRRGIERDKTRIQN 67
           +LLLL  L + PA   S   PR F  +L  VDA+     N T+ E+ RR I+R + R+  
Sbjct: 5   VLLLLLALAALPA---SCAPPRSFRLELASVDASAADAANLTEHELLRRAIQRSRYRLAG 64

Query: 68  F----------KKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCN 127
                      +K  V      P++   G YL+ L  GTPP  F+A  DT SDLIWTQC 
Sbjct: 65  IGMARGEAASARKAVV---AETPIMPAGGEYLVKLGIGTPPYKFTAAIDTASDLIWTQCQ 124

Query: 128 PCLKCFGQPTPVYDPAQSSSFTNATRSTALCQAL----TNSSSSNGCEYSYGYGDGSFTI 187
           PC  C+ Q  P+++P  SS++     S+  C  L            C+Y+Y Y   + T 
Sbjct: 125 PCTGCYHQVDPMFNPRVSSTYAALPCSSDTCDELDVHRCGHDDDESCQYTYTYSGNATTE 184

Query: 188 GYLAFETLTLGEANQQVSTPDIAFGCGIRNYVKG-LTQGAGIVGFSRGPLSLLSQLHIRK 247
           G LA + L +GE     +   +AFGC   +       Q +G+VG  RGPLSL+SQL +R+
Sbjct: 185 GTLAVDKLVIGED----AFRGVAFGCSTSSTGGAPPPQASGVVGLGRGPLSLVSQLSVRR 244

Query: 248 FSYCLSPNGT---GCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQK 307
           F+YCL P  +   G L  G+ + +  N TN      P+ ++P  PSYYYL+L G+ +G +
Sbjct: 245 FAYCLPPPASRIPGKLVLGADADAARNATNRIA--VPMRRDPRYPSYYYLNLDGLLIGDR 304

Query: 308 FL--------------------PVPSSWFELNADGSG---GMIIDSGTSITYITEDAFDV 367
            +                    P PS      A G     GMIID  ++IT++    +D 
Sbjct: 305 AMSLPPTTTTTATATATAPAPAPTPSPNATAVAVGDANRYGMIIDIASTITFLEASLYDE 364

Query: 368 LKPVFTSQTNLPVINSASIGLDLCFELP-SPNNSKLDVPDLIFHFEGLDLKLPVENYMVV 427
           L      +  LP    +S+GLDLCF LP      ++ VP +   F+G  L+L        
Sbjct: 365 LVNDLEVEIRLPRGTGSSLGLDLCFILPDGVAFDRVYVPAVALAFDGRWLRLDKARLFAE 424

Query: 428 NEEVGIVCLAMG--AAGGLSIFGSMQHQNMLVLHDLKKKVISFIPTQCSRI 430
           + E G++CL +G   AG +SI G+ Q QNM VL++L++  ++F+ + C  +
Sbjct: 425 DRESGMMCLMVGRAEAGSVSILGNFQQQNMQVLYNLRRGRVTFVQSPCGAL 463

BLAST of CcUC08G149690.1 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 4.2e-56
Identity = 145/446 (32.51%), Postives = 233/446 (52.24%), Query Frame = 0

Query: 9   LLLLPVLHSQPAFSESSLKPRFHFDLTHVDA------TQNFTKFEMFRRGIERDKTRIQN 68
           +LL   L      S S     F  +L H D+          T  +       R  +R + 
Sbjct: 5   ILLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRR 64

Query: 69  FKKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLKCFGQPT 128
           F       +++  L+   G + M+++ GTPP+   AI DTGSDL W QC PC +C+ +  
Sbjct: 65  FNHQLSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENG 124

Query: 129 PVYDPAQSSSFTNATRSTALCQALTNS-----SSSNGCEYSYGYGDGSFTIGYLAFETLT 188
           P++D  +SS++ +    +  CQAL+++      S+N C+Y Y YGD SF+ G +A ET++
Sbjct: 125 PIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVS 184

Query: 189 LGEAN-QQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLH---IRKFSYCLS 248
           +  A+   VS P   FGCG  N       G+GI+G   G LSL+SQL     +KFSYCLS
Sbjct: 185 IDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLS 244

Query: 249 -----PNGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPV 308
                 NGT  +  G++S     + +  V +TPL+ +    +YYYL+L+ I+VG+K +P 
Sbjct: 245 HKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLV-DKEPLTYYYLTLEAISVGKKKIPY 304

Query: 309 PSSWFELNADG-----SGGMIIDSGTSITYITEDAFDVL-KPVFTSQTNLPVINSASIGL 368
             S +  N DG     SG +IIDSGT++T +    FD     V  S T    ++     L
Sbjct: 305 TGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLL 364

Query: 369 DLCFELPSPNNSKLDVPDLIFHFEGLDLKL-PVENYMVVNEEVGIVCLAMGAAGGLSIFG 428
             CF+     ++++ +P++  HF G D++L P+  ++ ++E+  +VCL+M     ++I+G
Sbjct: 365 SHCFK---SGSAEIGLPEITVHFTGADVRLSPINAFVKLSED--MVCLSMVPTTEVAIYG 424

BLAST of CcUC08G149690.1 vs. ExPASy TrEMBL
Match: A0A2N9HF56 (Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS40859 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 7.8e-122
Identity = 241/438 (55.02%), Postives = 307/438 (70.09%), Query Frame = 0

Query: 6   FLLLLLLPVL-----HSQPAFSESSLKPRFHFDLTHVDA-TQNFTKFEMFRRG-IERDKT 65
           F LLLL+ +L      S   F ES  K  F   LT VDA  +N T+ ++ +R  I R KT
Sbjct: 10  FSLLLLVVILSLLSFSSGLPFDESPEKTGFRVSLTRVDAGKKNLTELQLVQRAIINRVKT 69

Query: 66  RIQNFKKMTVNF---EVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCL 125
           R+     +T N    +++ P+    G +LM+LS GTP V F AI DTGSDLIWTQC PC 
Sbjct: 70  RLDRLSAITKNAPNDDIQAPIYVGDGEFLMHLSIGTPAVPFLAIMDTGSDLIWTQCKPCS 129

Query: 126 KCFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFET 185
            CF Q TPV++PA SSSF+N T S+ LC+AL+NS   NGCEY   YGD S + G+LA ET
Sbjct: 130 NCFNQSTPVFEPALSSSFSNVTCSSELCKALSNSRCGNGCEYLNRYGDESSSEGFLAMET 189

Query: 186 LTLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLS-- 245
           LT GE+ Q+VS P+I FGCG+ N  KG  + AG+VG  RGPLSL+SQL  +KFSYCL+  
Sbjct: 190 LTFGESAQRVSVPNIGFGCGVDNKGKGWNKAAGLVGLGRGPLSLVSQLGSKKFSYCLTSI 249

Query: 246 -PNGTGCLAFGSSSTSFDNTTNLAV-KNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSS 305
             N +  L FGS +    N +N AV K TPLI+NP  PS+YYLSL+GITVGQ  LP+P S
Sbjct: 250 GDNKSSSLLFGSLADL--NLSNGAVLKTTPLIRNPILPSFYYLSLEGITVGQTLLPIPKS 309

Query: 306 WFELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSP 365
            F+L +DG GG+I+DSGT+IT + EDAFDVLK VF +QT L V N  + GLD+CF+LPS 
Sbjct: 310 LFQLGSDGGGGVILDSGTTITSLHEDAFDVLKQVFKAQTKLRVSNLYTRGLDVCFDLPSQ 369

Query: 366 NNSKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVL 425
           +   + VP L+FHFEGLDL+LPVENYM+ + ++G+VCLAMGA G LSIFG++Q QNMLVL
Sbjct: 370 DVKDIKVPKLVFHFEGLDLELPVENYMIADTKLGVVCLAMGATGRLSIFGNIQQQNMLVL 429

Query: 426 HDLKKKVISFIPTQCSRI 430
           HDL+K+ +SFIPT+C ++
Sbjct: 430 HDLEKETVSFIPTKCDQL 445

BLAST of CcUC08G149690.1 vs. ExPASy TrEMBL
Match: A0A438KMS9 (Aspartic proteinase nepenthesin-1 OS=Vitis vinifera OX=29760 GN=nep1_0 PE=3 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.7e-121
Identity = 231/436 (52.98%), Postives = 294/436 (67.43%), Query Frame = 0

Query: 6   FLLLLL----LPVLHS--QPAFSESSLKPRFHFDLTHVDATQNFTKFEMFRRGIERDKTR 65
           FLLL+L    LP   S   P F    L+  F   L H+DA +NFTK ++ +RGI R + R
Sbjct: 13  FLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTKLQLIQRGINRGRQR 72

Query: 66  IQNFKKMTVNFE---VRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLK 125
           +Q    M    E    + P+    G +++NL  GTPPV F AI DTGSDLIWTQCNPC  
Sbjct: 73  LQRMSGMATTAERNDFQPPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLIWTQCNPCKL 132

Query: 126 CFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFETL 185
           CF Q TPV++P +SS+F+N + S+ LC+ +  S     CEY Y YGD S T G++A +T+
Sbjct: 133 CFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESSTEGFMAMDTI 192

Query: 186 TLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLS--- 245
           T GE+ ++VS P I FGCG+ N   G+ Q AG++G  RG LSL+SQL  +KFSYCL+   
Sbjct: 193 TFGESPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQKFSYCLTSIH 252

Query: 246 PNGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSSWF 305
            N T  L FG  S ++ N     +  TPLIQNP  PSYYYLSL+GITVG   LP+P   F
Sbjct: 253 ENKTSSLLFG--SLAYSNFNPGKIPRTPLIQNPFLPSYYYLSLKGITVGYTLLPIPEFAF 312

Query: 306 ELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSPNN 365
           +L  DGSGGMI+DSGT+ITY+ EDAFDVLK  F SQT L V NS++ GLDLCF LP  N 
Sbjct: 313 QLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDLCFHLPVKNA 372

Query: 366 SKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVLHD 425
           +++ VP LIFHF+GLDL LPVENYMV + E+G++CLA+ A G LSIFG++Q QNMLVLHD
Sbjct: 373 AEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQQQNMLVLHD 432

Query: 426 LKKKVISFIPTQCSRI 430
           LKK  +S +PTQC ++
Sbjct: 433 LKKSTLSLVPTQCDKV 446

BLAST of CcUC08G149690.1 vs. ExPASy TrEMBL
Match: F6H0S5 (Peptidase A1 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001g03090 PE=3 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 1.1e-120
Identity = 229/436 (52.52%), Postives = 293/436 (67.20%), Query Frame = 0

Query: 6   FLLLLL----LPVLHS--QPAFSESSLKPRFHFDLTHVDATQNFTKFEMFRRGIERDKTR 65
           FLLL+L    LP   S   P F    L+  F   L H+DA +NFT+ ++ +RGI R + R
Sbjct: 13  FLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTRLQLIQRGINRGRQR 72

Query: 66  IQNFKKMTVNFE---VRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLK 125
           +Q    M    E    + P+    G +++NL  GTPPV F AI DTGSDLIWTQCNPC  
Sbjct: 73  LQRMSGMATTAERNGFQAPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLIWTQCNPCKL 132

Query: 126 CFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFETL 185
           CF Q TPV++P +SS+F+N + S+ LC+ +  S     CEY Y YGD S T G++A +T+
Sbjct: 133 CFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESSTEGFMAMDTI 192

Query: 186 TLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLS--- 245
           T GE  ++VS P I FGCG+ N   G+ Q AG++G  RG LSL+SQL  +KFSYCL+   
Sbjct: 193 TFGELPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQKFSYCLTSIH 252

Query: 246 PNGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSSWF 305
            N T  L FG  S ++ N     +  TPLIQNP  PSYYYL+L+GITVG   LP+P   F
Sbjct: 253 ENKTSSLLFG--SLAYSNFNPGKIPRTPLIQNPFLPSYYYLALKGITVGYTLLPIPEFAF 312

Query: 306 ELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSPNN 365
           +L  DGSGGMI+DSGT+ITY+ EDAFDVLK  F SQT L V NS++ GLDLCF LP  N 
Sbjct: 313 QLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDLCFHLPVKNA 372

Query: 366 SKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVLHD 425
           +++ VP LIFHF+GLDL LPVENYMV + E+G++CLA+ A G LSIFG++Q QNMLVLHD
Sbjct: 373 AEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQQQNMLVLHD 432

Query: 426 LKKKVISFIPTQCSRI 430
           LKK  +S +PTQC ++
Sbjct: 433 LKKSTLSLVPTQCDKV 446

BLAST of CcUC08G149690.1 vs. ExPASy TrEMBL
Match: A0A4U5QCA8 (Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000107380 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.6e-119
Identity = 226/441 (51.25%), Postives = 300/441 (68.03%), Query Frame = 0

Query: 1   MANCCFLLLLLLPVLHSQPAFSESS--------LKPRFHFDLTHVDATQNFTKFEMFRRG 60
           M + CF+L L +  +   PAFS S         L+  F   L HVD+ +N TK E  R G
Sbjct: 4   MTSLCFVLALAMFTIFFSPAFSTSRRALEHPKILQKGFRVRLKHVDSGKNLTKLERIRHG 63

Query: 61  IERDKTRIQNFKKMTV----NFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIW 120
           ++R + R+Q  K M +    + E+  P++   G +LM L+ GTPP ++SAI DTGSDLIW
Sbjct: 64  VKRGRNRLQRLKAMALVASSSSEIEAPVLPGNGEFLMKLAIGTPPETYSAILDTGSDLIW 123

Query: 121 TQCNPCLKCFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTI 180
           TQC PC +CF Q TP++DP +SSSF+  + S+ LC+AL  SS + GCEY Y YGD S T 
Sbjct: 124 TQCKPCTQCFDQSTPIFDPKKSSSFSKLSCSSQLCEALPQSSCNKGCEYQYSYGDYSSTQ 183

Query: 181 GYLAFETLTLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKF 240
           G LA ETLT G+A    S P++AFGCG  N   G +QGAG+VG  RGPLSL+SQL   KF
Sbjct: 184 GILASETLTFGKA----SVPNVAFGCGADNEGSGFSQGAGLVGLGRGPLSLVSQLKEPKF 243

Query: 241 SYCLS---PNGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKF 300
           SYCL+      T  L  GS ++   N ++ A+K TPLI +P+ PS+YYLSL+GI+VG   
Sbjct: 244 SYCLTTVDDTKTSTLLMGSLASV--NASSSAIKTTPLIHSPAQPSFYYLSLEGISVGDTR 303

Query: 301 LPVPSSWFELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLC 360
           LP+  S F L  DGSGG+IIDSGT+ITY+ E AFD++   FTSQ NLPV +S S GLD+C
Sbjct: 304 LPIKKSTFSLQDDGSGGLIIDSGTTITYLEESAFDLVAKEFTSQINLPVDSSGSTGLDVC 363

Query: 361 FELPSPNNSKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQH 420
           F LPS  ++ ++VP L+FHF+G DL+LP ENYM+ +  +G+ CLAMG++ G+SIFG++Q 
Sbjct: 364 FTLPS-GSTNMEVPKLVFHFDGADLELPAENYMIGDSSMGVACLAMGSSSGMSIFGNVQQ 423

Query: 421 QNMLVLHDLKKKVISFIPTQC 427
           QNMLVLHDL+K+ +SF+PT C
Sbjct: 424 QNMLVLHDLEKETLSFLPTPC 437

BLAST of CcUC08G149690.1 vs. ExPASy TrEMBL
Match: A0A4U5R6H0 (Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000004600 PE=3 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 1.0e-118
Identity = 217/418 (51.91%), Postives = 290/418 (69.38%), Query Frame = 0

Query: 17  SQPAFSESSLKPRFHFDLTHVDATQNFTKFEMFRRGIERDKTRIQNFKKMTV----NFEV 76
           S+       ++  F   L HVD+ +N TKFE  + G++R + R+Q FK M +    N E+
Sbjct: 27  SRRVLEHPKVRNGFRVKLKHVDSGKNLTKFERIQHGVKRGRHRLQRFKAMALVASSNSEI 86

Query: 77  RMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLKCFGQPTPVYDPAQSSS 136
             P++   G +LM L+ GTPP ++SAI DTGSDLIWTQC PC +CF QPTP++DP +SSS
Sbjct: 87  DAPVLPGNGEFLMKLAIGTPPETYSAIMDTGSDLIWTQCKPCTQCFDQPTPIFDPKKSSS 146

Query: 137 FTNATRSTALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFETLTLGEANQQVSTPDIAF 196
           F+  + S+ LC+AL  S+ S+GCEY YGYGD S T G LA ETLT G    +VS P +AF
Sbjct: 147 FSKLSCSSKLCEALPQSTCSDGCEYLYGYGDYSSTQGILASETLTFG----KVSVPKVAF 206

Query: 197 GCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCL-SPNGTGCLAFGSSSTSFDN 256
           GCG  N   G +QG+G+VG  RGPLSL+SQL   KFSYCL S + T        S +   
Sbjct: 207 GCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLEEPKFSYCLTSVDDTKASTLLMGSLASVK 266

Query: 257 TTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSSWFELNADGSGGMIIDSGTSI 316
            ++  +K TPLIQN + PS+YYLSL+GI+VG   LP+  S F L  DGSGG+IIDSGT+I
Sbjct: 267 ASDSEIKTTPLIQNSAQPSFYYLSLEGISVGDTSLPIKKSTFSLQEDGSGGLIIDSGTTI 326

Query: 317 TYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSPNNSKLDVPDLIFHFEGLDLK 376
           TY+ + AFD++   FTSQ NLPV NS S GL++CF LPS  ++ ++VP L+FHF+G DL+
Sbjct: 327 TYLEQSAFDLVAKEFTSQINLPVDNSGSTGLEVCFTLPS-GSTDIEVPKLVFHFDGADLE 386

Query: 377 LPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVLHDLKKKVISFIPTQCSRI 430
           LP ENYM+ +  +G+ CLAMG++ G+SIFG++Q QNMLVLHDL+K+ +SF+PTQC  +
Sbjct: 387 LPPENYMIADASMGVACLAMGSSSGMSIFGNIQQQNMLVLHDLEKETLSFLPTQCDEL 439

BLAST of CcUC08G149690.1 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 384.0 bits (985), Expect = 1.6e-106
Identity = 214/452 (47.35%), Postives = 281/452 (62.17%), Query Frame = 0

Query: 6   FLLLL--LLPVLHSQPAFSESSLK---PR--FHFDLTHVDATQNFTKFEMFRRGIERDKT 65
           FL+L   L+ V  S+ +  + +L    PR  F   L HVD+ +N TK +  +RGI R   
Sbjct: 14  FLILFSCLISVSSSRRSLIDRTLPKNLPRSGFRLSLRHVDSGKNLTKIQKIQRGINRGFH 73

Query: 66  RIQNFKKMTV---------NFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWT 125
           R+     + V            ++ P     G +LM LS G P V +SAI DTGSDLIWT
Sbjct: 74  RLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWT 133

Query: 126 QCNPCLKCFGQPTPVYDPAQSSSFTNATRSTALCQALTNSS---SSNGCEYSYGYGDGSF 185
           QC PC +CF QPTP++DP +SSS++    S+ LC AL  S+     + CEY Y YGD S 
Sbjct: 134 QCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNEDKDACEYLYTYGDYSS 193

Query: 186 TIGYLAFETLTLGEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIR 245
           T G LA ET T  + N   S   I FGCG+ N   G +QG+G+VG  RGPLSL+SQL   
Sbjct: 194 TRGLLATETFTFEDEN---SISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKET 253

Query: 246 KFSYCLS----PNGTGCLAFGSSSTSFDNTTNLAV-----KNTPLIQNPSNPSYYYLSLQ 305
           KFSYCL+       +  L  GS ++   N T  ++     K   L++NP  PS+YYL LQ
Sbjct: 254 KFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQ 313

Query: 306 GITVGQKFLPVPSSWFELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINS 365
           GITVG K L V  S FEL  DG+GGMIIDSGT+ITY+ E AF VLK  FTS+ +LPV +S
Sbjct: 314 GITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDS 373

Query: 366 ASIGLDLCFELPSPNNSKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGL 425
            S GLDLCF+LP    + + VP +IFHF+G DL+LP ENYMV +   G++CLAMG++ G+
Sbjct: 374 GSTGLDLCFKLPDAAKN-IAVPKMIFHFKGADLELPGENYMVADSSTGVLCLAMGSSNGM 433

Query: 426 SIFGSMQHQNMLVLHDLKKKVISFIPTQCSRI 430
           SIFG++Q QN  VLHDL+K+ +SF+PT+C ++
Sbjct: 434 SIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

BLAST of CcUC08G149690.1 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.6e-63
Identity = 157/445 (35.28%), Postives = 231/445 (51.91%), Query Frame = 0

Query: 6   FLLLLLLPVLHSQPAFSESSLKPRFHF--DLTHVDATQN------FTKFEMFRRGIERDK 65
           F  +LL   L S    S ++ KP+  F  DL H D+ ++       T  +  R  I R  
Sbjct: 5   FSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSV 64

Query: 66  TRIQNFKKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLKC 125
            R+ +F +     + ++ L    G YLMN+S GTPP    AI DTGSDL+WTQC PC  C
Sbjct: 65  NRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDC 124

Query: 126 FGQPTPVYDPAQSSSFTNATRSTALCQALTN----SSSSNGCEYSYGYGDGSFTIGYLAF 185
           + Q  P++DP  SS++ + + S++ C AL N    S++ N C YS  YGD S+T G +A 
Sbjct: 125 YTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAV 184

Query: 186 ETLTLGEAN-QQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIR---KFS 245
           +TLTLG ++ + +   +I  GCG  N      +G+GIVG   GP+SL+ QL      KFS
Sbjct: 185 DTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFS 244

Query: 246 YCLSP-----NGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQK 305
           YCL P     + T  + FG+++      +   V +TPLI   S  ++YYL+L+ I+VG K
Sbjct: 245 YCLVPLTSKKDQTSKINFGTNAI----VSGSGVVSTPLIAKASQETFYYLTLKSISVGSK 304

Query: 306 FLPVPSSWFELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDL 365
            +    S  E      G +IIDSGT++T +  + +  L+    S  +         GL L
Sbjct: 305 QIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSL 364

Query: 366 CFELPSPNNSKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQ 425
           C+         L VP +  HF+G D+KL   N  V   E  +VC A   +   SI+G++ 
Sbjct: 365 CYSA----TGDLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNVA 424

Query: 426 HQNMLVLHDLKKKVISFIPTQCSRI 430
             N LV +D   K +SF PT C+++
Sbjct: 425 QMNFLVGYDTVSKTVSFKPTDCAKM 437

BLAST of CcUC08G149690.1 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 224.2 bits (570), Expect = 2.1e-58
Identity = 138/387 (35.66%), Postives = 209/387 (54.01%), Query Frame = 0

Query: 60  IQNFKKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLKCFG 119
           I   ++ T   +++  L+   G Y M++S GTPP    AI DTGSDL W QC PC +C+ 
Sbjct: 62  ISRSRRFTTKTDLQSGLISNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPCQQCYK 121

Query: 120 QPTPVYDPAQSSSFTNATRSTALCQALTN-----SSSSNGCEYSYGYGDGSFTIGYLAFE 179
           Q +P++D  +SS++   +  +  CQAL+        S + C+Y Y YGD SFT G +A E
Sbjct: 122 QNSPLFDKKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTKGDVATE 181

Query: 180 TLTL-GEANQQVSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHI---RKFSY 239
           T+++   +   VS P   FGCG  N       G+GI+G   GPLSL+SQL     +KFSY
Sbjct: 182 TISIDSSSGSSVSFPGTVFGCGYNNGGTFEETGSGIIGLGGGPLSLVSQLGSSIGKKFSY 241

Query: 240 CLS-----PNGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQKF 299
           CLS      NGT  +  G++S   + + + A   TPLIQ     +YY+L+L+ +TVG+  
Sbjct: 242 CLSHTAATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDPE-TYYFLTLEAVTVGKTK 301

Query: 300 LPVPSSWFELNADGS---GGMIIDSGTSITYITEDAFDVL-KPVFTSQTNLPVINSASIG 359
           LP     + LN   S   G +IIDSGT++T +    +D     V  S T    ++     
Sbjct: 302 LPYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKRVSDPQGL 361

Query: 360 LDLCFELPSPNNSKLDVPDLIFHFEGLDLKL-PVENYMVVNEEVGIVCLAMGAAGGLSIF 419
           L  CF+     + ++ +P +  HF   D+KL P+  ++ +NE+   VCL+M     ++I+
Sbjct: 362 LTHCFK---SGDKEIGLPAITMHFTNADVKLSPINAFVKLNEDT--VCLSMIPTTEVAIY 421

Query: 420 GSMQHQNMLVLHDLKKKVISFIPTQCS 428
           G+M   + LV +DL+ K +SF    CS
Sbjct: 422 GNMVQMDFLVGYDLETKTVSFQRMDCS 442

BLAST of CcUC08G149690.1 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 222.6 bits (566), Expect = 6.0e-58
Identity = 158/442 (35.75%), Postives = 219/442 (49.55%), Query Frame = 0

Query: 20  AFSESSLKPRFHFDLTHVDATQNF---TKFEMFRRGIERDKTRIQNFKKMTV-----NFE 79
           + SES+     H  L+HVDA  +F   +  ++F   ++RD  R+++   +       N  
Sbjct: 53  SLSESTTSLSVH--LSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNAT 112

Query: 80  VRMP-------------LVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLKCF 139
            R P             L    G Y M L  GTP  +   + DTGSD++W QC+PC  C+
Sbjct: 113 KRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACY 172

Query: 140 GQPTPVYDPAQSSSFTNATRSTALCQALTNSSS-----SNGCEYSYGYGDGSFTIGYLAF 199
            Q   ++DP +S +F      + LC+ L +SS      S  C Y   YGDGSFT G  + 
Sbjct: 173 NQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFST 232

Query: 200 ETLTLGEANQQVSTPDIAFGCGIRNYVKGLTQG-AGIVGFSRGPLSLLSQLHIR---KFS 259
           ETLT   A        +  GCG  N  +GL  G AG++G  RG LS  SQ   R   KFS
Sbjct: 233 ETLTFHGAR----VDHVPLGCGHDN--EGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFS 292

Query: 260 YCL-SPNGTGCLAFGSSSTSFDNTTNLAVK-NTPLIQNPSNPSYYYLSLQGITVGQKFLP 319
           YCL     +G  +   S+  F N         TPL+ NP   ++YYL L GI+VG   +P
Sbjct: 293 YCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVP 352

Query: 320 -VPSSWFELNADGSGGMIIDSGTSITYITEDAFDVLKPVF-TSQTNLPVINSASIGLDLC 379
            V  S F+L+A G+GG+IIDSGTS+T +T+ A+  L+  F    T L    S S+  D C
Sbjct: 353 GVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSL-FDTC 412

Query: 380 FELPSPNNSKLDVPDLIFHFEGLDLKLPVENYMVVNEEVGIVCLAM-GAAGGLSIFGSMQ 427
           F+L      K  VP ++FHF G ++ LP  NY++     G  C A  G  G LSI G++Q
Sbjct: 413 FDLSGMTTVK--VPTVVFHFGGGEVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQ 472

BLAST of CcUC08G149690.1 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 222.2 bits (565), Expect = 7.8e-58
Identity = 152/447 (34.00%), Postives = 241/447 (53.91%), Query Frame = 0

Query: 1   MANCCFLLLLLLPVLHSQPAFSESSLKPRFHFDLTHVDATQNFTKFEMFRRGIERDKTRI 60
           MA+  F  LL L +L +  A+     K  F  DL H D+ ++   +       +R +  I
Sbjct: 1   MASLIFATLLSLLLLSNVNAYP----KDGFTIDLIHRDSPKS-PFYNSAETSSQRMRNAI 60

Query: 61  QNFKKMTVNF--------EVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCN 120
           +   + T+ F          +  +   +G YLMN+S GTPPV   AI DTGSDLIWTQCN
Sbjct: 61  RRSARSTLQFSNDDASPNSPQSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCN 120

Query: 121 PCLKCFGQPTPVYDPAQSSSFTNATRSTALCQALTNSSSS---NGCEYSYGYGDGSFTIG 180
           PC  C+ Q +P++DP +SS++   + S++ C+AL ++S S   N C Y+  YGD S+T G
Sbjct: 121 PCEDCYQQTSPLFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKG 180

Query: 181 YLAFETLTLGEANQQ-VSTPDIAFGCGIRNYVKGLTQGAGIVGFSRGPLSLLSQLHIR-- 240
            +A +T+T+G + ++ VS  ++  GCG  N       G+GI+G   G  SL+SQL     
Sbjct: 181 DVAVDTVTMGSSGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSIN 240

Query: 241 -KFSYCLSP--NGTGCLAFGSSSTSFDNTTNLAVKNTPLIQNPSNPSYYYLSLQGITVGQ 300
            KFSYCL P  + TG  +  +  T+   + +  V  + + ++P+  +YY+L+L+ I+VG 
Sbjct: 241 GKFSYCLVPFTSETGLTSKINFGTNGIVSGDGVVSTSMVKKDPA--TYYFLNLEAISVGS 300

Query: 301 KFLPVPSSWFELNADGSGGMIIDSGTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLD 360
           K +   S+ F     G G ++IDSGT++T +  + +  L+ V  S      +      L 
Sbjct: 301 KKIQFTSTIF---GTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILS 360

Query: 361 LCFELPSPNNSKLDVPDLIFHFEGLDLKL-PVENYMVVNEEVGIVCLAMGAAGGLSIFGS 420
           LC+     ++S   VPD+  HF+G D+KL  +  ++ V+E+V   C A  A   L+IFG+
Sbjct: 361 LCYR----DSSSFKVPDITVHFKGGDVKLGNLNTFVAVSEDVS--CFAFAANEQLTIFGN 420

Query: 421 MQHQNMLVLHDLKKKVISFIPTQCSRI 430
           +   N LV +D     +SF  T CS++
Sbjct: 421 LAQMNFLVGYDTVSGTVSFKKTDCSQM 431

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886455.12.3e-20584.03aspartic proteinase nepenthesin-2-like [Benincasa hispida][more]
RVX22508.13.6e-12152.98Aspartic proteinase nepenthesin-1 [Vitis vinifera][more]
XP_034676400.11.8e-12052.52aspartic proteinase nepenthesin-1-like [Vitis riparia][more]
XP_010664223.12.3e-12052.52PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera][more]
XP_011045692.15.2e-12051.59PREDICTED: aspartic proteinase nepenthesin-1-like [Populus euphratica][more]
Match NameE-valueIdentityDescription
Q766C33.4e-9846.68Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C22.7e-9545.21Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q6XBF82.3e-6235.28Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q7XV215.8e-5833.55Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2[more]
Q3EBM54.2e-5632.51Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561... [more]
Match NameE-valueIdentityDescription
A0A2N9HF567.8e-12255.02Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4... [more]
A0A438KMS91.7e-12152.98Aspartic proteinase nepenthesin-1 OS=Vitis vinifera OX=29760 GN=nep1_0 PE=3 SV=1[more]
F6H0S51.1e-12052.52Peptidase A1 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001... [more]
A0A4U5QCA81.6e-11951.25Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000107380 PE... [more]
A0A4U5R6H01.0e-11851.91Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000004600 PE... [more]
Match NameE-valueIdentityDescription
AT2G03200.11.6e-10647.35Eukaryotic aspartyl protease family protein [more]
AT5G33340.11.6e-6335.28Eukaryotic aspartyl protease family protein [more]
AT1G31450.12.1e-5835.66Eukaryotic aspartyl protease family protein [more]
AT3G61820.16.0e-5835.75Eukaryotic aspartyl protease family protein [more]
AT1G64830.17.8e-5834.00Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 303..314
score: 40.02
coord: 89..109
score: 46.28
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 57..244
e-value: 1.3E-49
score: 170.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 245..431
e-value: 1.7E-50
score: 173.2
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 78..428
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 272..421
e-value: 1.6E-28
score: 99.5
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 83..244
e-value: 7.5E-45
score: 153.3
NoneNo IPR availablePANTHERPTHR47967:SF23OS08G0469000 PROTEINcoord: 14..427
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 14..427
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 303..314
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 83..422
score: 37.569767
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 83..426
e-value: 1.56334E-97
score: 291.089

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CcUC08G149690CcUC08G149690gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC08G149690.1-exonCcUC08G149690.1-exon-CicolChr08:17477806..17479101exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC08G149690.1-cdsCcUC08G149690.1-cds-CicolChr08:17477806..17479101CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CcUC08G149690.1CcUC08G149690.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004190 aspartic-type endopeptidase activity