CcUC08G148750.1 (mRNA) Watermelon (PI 537277) v1

Overview
NameCcUC08G148750.1
TypemRNA
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionrRNA N-glycosidase
LocationCicolChr08: 13435485 .. 13437125 (+)
Sequence length1641
RNA-Seq ExpressionCcUC08G148750.1
SyntenyCcUC08G148750.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGTTTTAGTAGCCTTCATTGTAGCACTTTGCCTTACCACTGATGGCATTTATGCAGGAAATGCAAATCTGCATTTAACAAAGAGACCCGTGGTTGATGTTTACAAAAGTTTTATCCAAAATGTGCGTCAACAATTTGGGTCTAAAACCCATAAACTCTATGGCATTCCTGTCTTGCAACACTCTTTATCCAATTCAGATCGTTTCTATTTCGTCGATACCGGTAACGTAGCTGGCGATACGATCACTCTAGCAGTAGATGCAGAGGATATGAGTGTAGTTGCATATCTTGCTGGAGATAATGATTCTTACTTTTTTGCAAATGCTCCTCAATTAGCCTTTGATATTCTCTTCTCTAATACCCTTAAAACAATTTTGAGTTTCAACAACACCTTTCAATCCATTGAAATTGCAGCTAATACTACAAGACAAGCAACTCCCCTTGGCCTCCAATCTACTAACTCTGCCATCTCCAACTTGTTTCATTACAATCCTCATCTTGCTCCTGTTTCATTTCTTATCATCTTTCAAATGATCTTCGAAGCTGCGAAATTCAAATTCATCGAACAAGCCGTTGTCAACAGCATAGAGAATGGAGAAAGCTTCACACCCAACCTTGCAATTGTCAGCTTAGAGGACAATTGGTCAGAACTTTCTTTACAAATCCAAGCATCAAGTTCATTACAAGGACTATTTGGGAGTAGTGTAATGCTTTACAACTCTAGAAATGAAGTTATTGAAGTCGATAGTATATACTATCCAATCATACTTCCTAATCTTGCCTTGCAATTATACCATTGCAATACGCAAGATTACATCCAGATGCCTGGCGTTGTTCGACCTGACAATTCTCGATGTTATGTCGAGGAACGAACGACGTGGATTAGCGGTCGAGACGGACTTTGTGCAAATGTAGCTCATGCGTTGTCTAATGACCATTCTCTCGTTATTTTGTTCCCATGTGGAGAACAAGTCAATCAACAATGGACATTCCATAGCGACCACACGATTCGATCCTTAGGGAAATGTTTGATCCCAAGTAAATCCATCTCCGGAAGTCCTCTGGCTGTGATTTACAATTGTAGTAAAATCACCAAGCAAGATGCTACTTGGGAGGTTTCTATTAGTGGGACAATCATGAACTCAGCTTATGATCTTGTCTTGACTTCAAATAATGCAAGCAAGAGCAGCAACTTGACAATGGAGAATAATATATATGGTGGTAGTCAAGGGTGGAGAGTTGGGAATTATGTGGATCCAATTATAACTTCCATTATAGGAGTTAAACAAATGTGTTTGGAGGCTAAGGATGAAAATACAAACATTTGGTTGGAAGAATGTGTGAAGAACAAGAGTAAGCAATCTTGGGCAGTTTATAGTGATGGAAGTATTAGAGTGAGCAATAATCCTAGCCTTTGTGTGACTGCCTCTTCAATTGAATCAAATCAGGTAATTGTCATTCTTAGATGCAATGGCTTAGCTTCCCAACGTTGGGTTTTCAAGGTGGATGGCACAATCTCACCTGCAAAGAATGAGAGCTTGGCAATGGATGTGGCACAAAATAACGTTGATCTCAAGAGAATCGTTTTATATCCTCGACATGGTTTGGTTAGCCAACAGTGGACGGTTTTCTACTAA

mRNA sequence

ATGAGAGTTTTAGTAGCCTTCATTGTAGCACTTTGCCTTACCACTGATGGCATTTATGCAGGAAATGCAAATCTGCATTTAACAAAGAGACCCGTGGTTGATGTTTACAAAAGTTTTATCCAAAATGTGCGTCAACAATTTGGGTCTAAAACCCATAAACTCTATGGCATTCCTGTCTTGCAACACTCTTTATCCAATTCAGATCGTTTCTATTTCGTCGATACCGGTAACGTAGCTGGCGATACGATCACTCTAGCAGTAGATGCAGAGGATATGAGTGTAGTTGCATATCTTGCTGGAGATAATGATTCTTACTTTTTTGCAAATGCTCCTCAATTAGCCTTTGATATTCTCTTCTCTAATACCCTTAAAACAATTTTGAGTTTCAACAACACCTTTCAATCCATTGAAATTGCAGCTAATACTACAAGACAAGCAACTCCCCTTGGCCTCCAATCTACTAACTCTGCCATCTCCAACTTGTTTCATTACAATCCTCATCTTGCTCCTGTTTCATTTCTTATCATCTTTCAAATGATCTTCGAAGCTGCGAAATTCAAATTCATCGAACAAGCCGTTGTCAACAGCATAGAGAATGGAGAAAGCTTCACACCCAACCTTGCAATTGTCAGCTTAGAGGACAATTGGTCAGAACTTTCTTTACAAATCCAAGCATCAAGTTCATTACAAGGACTATTTGGGAGTAGTGTAATGCTTTACAACTCTAGAAATGAAGTTATTGAAGTCGATAGTATATACTATCCAATCATACTTCCTAATCTTGCCTTGCAATTATACCATTGCAATACGCAAGATTACATCCAGATGCCTGGCGTTGTTCGACCTGACAATTCTCGATGTTATGTCGAGGAACGAACGACGTGGATTAGCGGTCGAGACGGACTTTGTGCAAATGTAGCTCATGCGTTGTCTAATGACCATTCTCTCGTTATTTTGTTCCCATGTGGAGAACAAGTCAATCAACAATGGACATTCCATAGCGACCACACGATTCGATCCTTAGGGAAATGTTTGATCCCAAGTAAATCCATCTCCGGAAGTCCTCTGGCTGTGATTTACAATTGTAGTAAAATCACCAAGCAAGATGCTACTTGGGAGGTTTCTATTAGTGGGACAATCATGAACTCAGCTTATGATCTTGTCTTGACTTCAAATAATGCAAGCAAGAGCAGCAACTTGACAATGGAGAATAATATATATGGTGGTAGTCAAGGGTGGAGAGTTGGGAATTATGTGGATCCAATTATAACTTCCATTATAGGAGTTAAACAAATGTGTTTGGAGGCTAAGGATGAAAATACAAACATTTGGTTGGAAGAATGTGTGAAGAACAAGAGTAAGCAATCTTGGGCAGTTTATAGTGATGGAAGTATTAGAGTGAGCAATAATCCTAGCCTTTGTGTGACTGCCTCTTCAATTGAATCAAATCAGGTAATTGTCATTCTTAGATGCAATGGCTTAGCTTCCCAACGTTGGGTTTTCAAGGTGGATGGCACAATCTCACCTGCAAAGAATGAGAGCTTGGCAATGGATGTGGCACAAAATAACGTTGATCTCAAGAGAATCGTTTTATATCCTCGACATGGTTTGGTTAGCCAACAGTGGACGGTTTTCTACTAA

Coding sequence (CDS)

ATGAGAGTTTTAGTAGCCTTCATTGTAGCACTTTGCCTTACCACTGATGGCATTTATGCAGGAAATGCAAATCTGCATTTAACAAAGAGACCCGTGGTTGATGTTTACAAAAGTTTTATCCAAAATGTGCGTCAACAATTTGGGTCTAAAACCCATAAACTCTATGGCATTCCTGTCTTGCAACACTCTTTATCCAATTCAGATCGTTTCTATTTCGTCGATACCGGTAACGTAGCTGGCGATACGATCACTCTAGCAGTAGATGCAGAGGATATGAGTGTAGTTGCATATCTTGCTGGAGATAATGATTCTTACTTTTTTGCAAATGCTCCTCAATTAGCCTTTGATATTCTCTTCTCTAATACCCTTAAAACAATTTTGAGTTTCAACAACACCTTTCAATCCATTGAAATTGCAGCTAATACTACAAGACAAGCAACTCCCCTTGGCCTCCAATCTACTAACTCTGCCATCTCCAACTTGTTTCATTACAATCCTCATCTTGCTCCTGTTTCATTTCTTATCATCTTTCAAATGATCTTCGAAGCTGCGAAATTCAAATTCATCGAACAAGCCGTTGTCAACAGCATAGAGAATGGAGAAAGCTTCACACCCAACCTTGCAATTGTCAGCTTAGAGGACAATTGGTCAGAACTTTCTTTACAAATCCAAGCATCAAGTTCATTACAAGGACTATTTGGGAGTAGTGTAATGCTTTACAACTCTAGAAATGAAGTTATTGAAGTCGATAGTATATACTATCCAATCATACTTCCTAATCTTGCCTTGCAATTATACCATTGCAATACGCAAGATTACATCCAGATGCCTGGCGTTGTTCGACCTGACAATTCTCGATGTTATGTCGAGGAACGAACGACGTGGATTAGCGGTCGAGACGGACTTTGTGCAAATGTAGCTCATGCGTTGTCTAATGACCATTCTCTCGTTATTTTGTTCCCATGTGGAGAACAAGTCAATCAACAATGGACATTCCATAGCGACCACACGATTCGATCCTTAGGGAAATGTTTGATCCCAAGTAAATCCATCTCCGGAAGTCCTCTGGCTGTGATTTACAATTGTAGTAAAATCACCAAGCAAGATGCTACTTGGGAGGTTTCTATTAGTGGGACAATCATGAACTCAGCTTATGATCTTGTCTTGACTTCAAATAATGCAAGCAAGAGCAGCAACTTGACAATGGAGAATAATATATATGGTGGTAGTCAAGGGTGGAGAGTTGGGAATTATGTGGATCCAATTATAACTTCCATTATAGGAGTTAAACAAATGTGTTTGGAGGCTAAGGATGAAAATACAAACATTTGGTTGGAAGAATGTGTGAAGAACAAGAGTAAGCAATCTTGGGCAGTTTATAGTGATGGAAGTATTAGAGTGAGCAATAATCCTAGCCTTTGTGTGACTGCCTCTTCAATTGAATCAAATCAGGTAATTGTCATTCTTAGATGCAATGGCTTAGCTTCCCAACGTTGGGTTTTCAAGGTGGATGGCACAATCTCACCTGCAAAGAATGAGAGCTTGGCAATGGATGTGGCACAAAATAACGTTGATCTCAAGAGAATCGTTTTATATCCTCGACATGGTTTGGTTAGCCAACAGTGGACGGTTTTCTACTAA

Protein sequence

MRVLVAFIVALCLTTDGIYAGNANLHLTKRPVVDVYKSFIQNVRQQFGSKTHKLYGIPVLQHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQLAFDILFSNTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIFQMIFEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSVMLYNSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIQMPGVVRPDNSRCYVEERTTWISGRDGLCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAVIYNCSKITKQDATWEVSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRVGNYVDPIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTASSIESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLVSQQWTVFY
Homology
BLAST of CcUC08G148750.1 vs. NCBI nr
Match: XP_038884532.1 (seed lectin-like [Benincasa hispida])

HSP 1 Score: 795.4 bits (2053), Expect = 3.0e-226
Identity = 410/547 (74.95%), Postives = 466/547 (85.19%), Query Frame = 0

Query: 1   MRVLVAFIVALCLTTDGIYAGNANLHLTKRPVVDVYKSFIQNVRQQFGSKTHK-LYGIPV 60
           MRVLVA I+ALC+  DGI A        K P+ D YK++IQNVRQ+F S+THK LYGIP+
Sbjct: 15  MRVLVALIIALCIAIDGITA-------AKTPISDRYKTYIQNVRQKFASQTHKQLYGIPM 74

Query: 61  LQHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQLAFDILF 120
           LQHSLSNSDRF  +DT NVAGDTITLAVDA+DMSVVAYLAGDNDSYFF+NAP   FDILF
Sbjct: 75  LQHSLSNSDRFLLIDTANVAGDTITLAVDAQDMSVVAYLAGDNDSYFFSNAPLFTFDILF 134

Query: 121 SNTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIFQM 180
            NT +T+LS++NTF+SIE AANTTRQA PLGL  +NSAISNLFHY P LA VSFL++FQM
Sbjct: 135 PNTHQTLLSYDNTFESIENAANTTRQAIPLGLNPSNSAISNLFHYRPDLAAVSFLVVFQM 194

Query: 181 IFEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSVML 240
           IFEAAKFKFIEQ +VN I+NGESFTPNLAIVSLEDNWS+LSLQIQ+S+SLQGLFGSSVML
Sbjct: 195 IFEAAKFKFIEQTIVNCIKNGESFTPNLAIVSLEDNWSQLSLQIQSSTSLQGLFGSSVML 254

Query: 241 YNSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIQMPGVVRPDNSRCYVEERTTWISGR 300
           +NS+NE IEVDSIY+PIIL NLALQLYHCNTQDYI++P +V  +NSRCYVEE TT ISGR
Sbjct: 255 HNSKNEAIEVDSIYFPIILNNLALQLYHCNTQDYIKLPSLV--ENSRCYVEEHTTRISGR 314

Query: 301 DGLCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAVI 360
           DGLCA+VA     D S VI FPC +Q NQQWTF+SD TIRSLGKCLIPSKS S +PLAVI
Sbjct: 315 DGLCADVA-----DGSHVISFPCRQQANQQWTFYSDRTIRSLGKCLIPSKS-SSNPLAVI 374

Query: 361 YNCSKITKQDATWEVSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRVGNYV 420
           Y+CSK+++QDATWEVS+SGTIMN+  D VLTSNN    S LTME+N Y G+QGWRVGN+V
Sbjct: 375 YDCSKVSQQDATWEVSLSGTIMNTYNDSVLTSNN---GSTLTMEDNAYDGNQGWRVGNFV 434

Query: 421 DPIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTASS 480
           DPI+TSIIGVK+MCLEA + NTNIWLEECVK+K +QSW +YSDGSIRV+NN SLCVTA S
Sbjct: 435 DPIVTSIIGVKEMCLEATEGNTNIWLEECVKDKIEQSWTLYSDGSIRVNNNHSLCVTA-S 494

Query: 481 IESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLVS 540
           I S Q IVIL CNGLA+QRWVFK DGTIS  K++ L MDVAQN+VDLKRIVLYPRHG V+
Sbjct: 495 IHSRQRIVILNCNGLATQRWVFKADGTISTPKDDGLVMDVAQNDVDLKRIVLYPRHGGVN 542

Query: 541 QQWTVFY 547
           QQW VFY
Sbjct: 555 QQWVVFY 542

BLAST of CcUC08G148750.1 vs. NCBI nr
Match: XP_004140357.1 (seed lectin [Cucumis sativus] >KGN51070.1 hypothetical protein Csa_008446 [Cucumis sativus])

HSP 1 Score: 779.6 bits (2012), Expect = 1.7e-221
Identity = 393/548 (71.72%), Postives = 458/548 (83.58%), Query Frame = 0

Query: 1   MRVLVAFIVALCLTTDGIYAGNANLHLTKRPVVDVYKSFIQNVRQQFGSKTHKLYGIPVL 60
           MRVL+AFIV L + T+          L+KRPVVDVY+S+IQ +RQ+FGSKTHKLYGIPVL
Sbjct: 1   MRVLLAFIVTLSIATNA---------LSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVL 60

Query: 61  QHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQLAFDILFS 120
            HSLSNSDRF+ +D GN  GDTIT AVDA+DMSVVAYLAGDNDSYFF+NAP+ AFDILF 
Sbjct: 61  HHSLSNSDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFP 120

Query: 121 NTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIFQMI 180
            T + +L+F+N+F+SIEIAANTTR+ATPLGL+ +N+AI+NLFHY+P LAPVSFLI+FQM+
Sbjct: 121 KTNQNLLNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMV 180

Query: 181 FEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSVMLY 240
           FE+AKFKFIEQ +VNSI NGE+FTP+LA++SLEDNWSELSL+IQAS+SLQGLFGSSV LY
Sbjct: 181 FESAKFKFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLY 240

Query: 241 NSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIQMPGVVRPD--NSRCYVEERTTWISG 300
           NS+NE +EVDSIYYP+IL NLALQLYHCN QDYI+MP     D  N RCYV+ERT  ISG
Sbjct: 241 NSKNEAVEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISG 300

Query: 301 RDGLCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAV 360
           +DGLCA+VA     D S VI  PCG+Q NQQWTFH DHTIRS  KCLIP+KS   +PLAV
Sbjct: 301 QDGLCADVA----RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKS-KANPLAV 360

Query: 361 IYNCSKITKQDATWEVSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRVGNY 420
           I NC+K++++DATW+VSISGTIMN AYDLVLTS N    ++L+M+ N Y G+QGWRVGNY
Sbjct: 361 IQNCNKVSQEDATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNY 420

Query: 421 VDPIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTAS 480
           V+PII SIIGVKQMCLEA +ENTNIWLEECVKNK +QSWAV+SDGSIRV+N+ SLC+TAS
Sbjct: 421 VEPIIASIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTAS 480

Query: 481 SIESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLV 540
           SIES Q IVI +CNGLASQRWV K DGTIS  K E L MDVAQ+NVDLK IVLYPR  LV
Sbjct: 481 SIESKQRIVIAKCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLV 534

Query: 541 SQQWTVFY 547
           SQ W   Y
Sbjct: 541 SQHWVALY 534

BLAST of CcUC08G148750.1 vs. NCBI nr
Match: XP_008463192.1 (PREDICTED: nigrin b-like [Cucumis melo] >KAA0062872.1 nigrin b-like [Cucumis melo var. makuwa] >TYK22504.1 nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 768.8 bits (1984), Expect = 3.0e-218
Identity = 390/548 (71.17%), Postives = 456/548 (83.21%), Query Frame = 0

Query: 1   MRVLVAFIVALCLTTDGIYAGNANLHLTKRPVVDVYKSFIQNVRQQFGSKTHKLYGIPVL 60
           MRVL+AF+V L   T+          L+KRPVVDVY+S+IQ +RQ+FGSKTHKLYGIPVL
Sbjct: 1   MRVLLAFVVTLSFATNA---------LSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVL 60

Query: 61  QHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQLAFDILFS 120
           QHSLSNSDRF+ +DTGN  GDTITLAVDA+DMSVVAYLAG++DSYFF+NAP+ AFDILF 
Sbjct: 61  QHSLSNSDRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFP 120

Query: 121 NTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIFQMI 180
           NT + +L+F+++F+SIE AANTTRQATPLG + +N+AI+NLFHY+  LAPVSFLI+FQMI
Sbjct: 121 NTNQNLLNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMI 180

Query: 181 FEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSVMLY 240
           FE+AKF FIEQ +VNSI+ GE+FTP+LA++SLEDNWSELSLQIQAS+SLQGLFGSSV LY
Sbjct: 181 FESAKFNFIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLY 240

Query: 241 NSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIQMPGVVRPD--NSRCYVEERTTWISG 300
           NS+NE IEVDSIYYP+IL NLALQLYHCN QDYI+MP     D  N RCYV+ERT  ISG
Sbjct: 241 NSKNEAIEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISG 300

Query: 301 RDGLCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAV 360
           RDGLC +VA     D S VI  PCG+Q NQ+WTF+ DHTIRS  KCLIP+KS   +P AV
Sbjct: 301 RDGLCVDVA----RDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKS-KANPFAV 360

Query: 361 IYNCSKITKQDATWEVSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRVGNY 420
           I +C+K+++QDATW+VSISGTIMN AYDLVLTSNN    ++L+M+ N Y G+QGWRVGN+
Sbjct: 361 ILDCNKVSQQDATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNF 420

Query: 421 VDPIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTAS 480
           V+P+I SIIGVKQMCLEA +ENTNIWLEECVKNK +QSWAVYSDGSIRV+N+ SLCVTAS
Sbjct: 421 VEPLIVSIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTAS 480

Query: 481 SIESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLV 540
           SIE  Q IVI +CNGLASQRWV   DGTIS  KNE L MDVAQ+NVDLK IVLYPR  LV
Sbjct: 481 SIEPKQRIVIGKCNGLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLV 534

Query: 541 SQQWTVFY 547
           SQQW   Y
Sbjct: 541 SQQWVALY 534

BLAST of CcUC08G148750.1 vs. NCBI nr
Match: U3KRF8.2 (RecName: Full=Seed lectin; Short=SGSL; Contains: RecName: Full=Seed lectin Aalpha chain; Contains: RecName: Full=Seed lectin Abeta chain; Contains: RecName: Full=Seed lectin B chain [Trichosanthes anguina])

HSP 1 Score: 609.8 bits (1571), Expect = 2.4e-170
Identity = 315/528 (59.66%), Postives = 404/528 (76.52%), Query Frame = 0

Query: 23  ANLHLTKRPVVDVYKSFIQNVRQQFGSKTHKLYGIPVLQHSLSNSDRFYFVDTGNVAGDT 82
           ANL L++      YK+FI  VR++ GS+T++LYGIPVL+HSLSNS+RFY +   +   ++
Sbjct: 4   ANLRLSEAN-SGTYKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQDES 63

Query: 83  ITLAVDAEDMSVVAYL-AGDNDSYFFANAPQLAFDILFSNTLKTILSFNNTFQSIEIAAN 142
           ITLA+D EDM  VAY  AG ++SYFF NAPQ+AF  LF++T + +L+F+NTF+S+E AA 
Sbjct: 64  ITLAIDVEDMVAVAYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENAAG 123

Query: 143 TTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIFQMIFEAAKFKFIEQAVVNSIENGE 202
           TTRQ   LG+   + AISNLF+ +P L P+SFL+I QM+ EA+KF+FIEQ+V  S +N +
Sbjct: 124 TTRQTIVLGVDPLDFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKNEK 183

Query: 203 SFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSVMLYNSRNEVIEVDSIYYPIILPNL 262
           +F P+LAIVSLEDNWSE+SLQIQAS+SLQGLFGS V LYNS NE+IEVDSIYYPIIL N+
Sbjct: 184 TFLPDLAIVSLEDNWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILANV 243

Query: 263 ALQLYHCNTQDYIQMPGVVRPDNSRCYVEERTTWISGRDGLCANVAHALSNDHSLVILFP 322
           ALQLYHC           V   ++ C VE RTT ISGRD LC +VA AL++D S +IL+P
Sbjct: 244 ALQLYHCQ----------VSTGDNECLVETRTTRISGRDALCVDVAGALTSDGSRLILYP 303

Query: 323 CGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAVIYNCSKITKQDATWEVSISGTIM 382
           CG+QVNQ+WTFHSD T+RSLGKCL  + S  G+ L VIY+CSK+  +D +W+VS+ GTIM
Sbjct: 304 CGQQVNQKWTFHSDGTVRSLGKCLATNNSKFGN-LVVIYDCSKLAAEDISWDVSVGGTIM 363

Query: 383 NSAY-DLVLTSNNASKSSNLTMENNIYGGSQGWRVGNYVDPIITSIIGVKQMCLEAKDEN 442
           N  Y DL LTSN A++S+NLTME N Y  SQGWRVGNYV PII SI+G+  MCLEA D N
Sbjct: 364 NPNYEDLALTSNKATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDGN 423

Query: 443 TNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTASS--IESNQVIVILRCNGLASQR 502
           TN+WLEECV N+ +QSWA+YSDG+IRV +N  LCVTASS   ++ +VI IL C+G  +QR
Sbjct: 424 TNMWLEECVPNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSNNQR 483

Query: 503 WVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLVSQQWTVFY 547
           WVF  DG+IS   N+ LAMDVA+++VDLK+I+L+  HG ++QQW +FY
Sbjct: 484 WVFLADGSISTPGNQRLAMDVARSDVDLKKIILHRPHGDLNQQWVLFY 519

BLAST of CcUC08G148750.1 vs. NCBI nr
Match: XP_022156703.1 (seed lectin-like [Momordica charantia] >BAH05018.1 galactose-binding type-2 ribosome-inactivating protein [Momordica charantia])

HSP 1 Score: 537.0 bits (1382), Expect = 1.9e-148
Identity = 293/552 (53.08%), Postives = 389/552 (70.47%), Query Frame = 0

Query: 1   MRVLVAFI-VALCLTTDGIYAGNANLHLTKRPV-VDVYKSFIQNVRQQFG-SKTHKLYGI 60
           MRVL  +I VAL LT +GI     NL L++     D YKSFI+N+R+Q     ++   GI
Sbjct: 3   MRVLAVYIVVALSLTINGI---ECNLSLSQSNFSADTYKSFIKNLRKQLTIGASYGSAGI 62

Query: 61  PVLQHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQLAFDI 120
           P+L+HS+   +RF  VD  N   +TITLA++ ED    AY A D  SYFF NAP +A  +
Sbjct: 63  PILKHSVPICERFLLVDLTNGDNETITLAINVEDAGFAAYRAADR-SYFFQNAPPIASYV 122

Query: 121 LFSNTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIF 180
           +F++T + I++FNNTF+SIEI   TTR  TPLG+    ++I +LF ++ +  P SFL++ 
Sbjct: 123 IFTDTNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVPTSFLVLI 182

Query: 181 QMIFEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSV 240
           QM+ EAAKFKFIEQ V++SI + E FTP LA++SLE+NW++LSLQ+QAS SL G+FG SV
Sbjct: 183 QMVLEAAKFKFIEQKVIHSIMDMEDFTPGLAMLSLEENWTQLSLQLQASESLNGVFGDSV 242

Query: 241 MLYNSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIQMPGVVRP--DNSRCYVEERTTW 300
            LYNS +E I VDS+YYPI+  N+A QLY C       MP +  P  +N +C  ++RTT 
Sbjct: 243 SLYNSMDEPIGVDSMYYPILTANMAFQLYQCPYGVIRMMPTITMPNQNNEQCSPQQRTTR 302

Query: 301 ISGRDGLCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSP 360
           ISGRDGLC +V  AL+ D S VIL+PCG+Q NQQWTF+ D+TIRSLGKCL  S   SGS 
Sbjct: 303 ISGRDGLCVDVYGALTADGSRVILYPCGQQQNQQWTFYPDNTIRSLGKCLATSALSSGSN 362

Query: 361 LAVIYNCSKITKQDATWEVSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRV 420
           + VI NC  + + D  W VS SGT+MN +  LVLT+N A+  +NLT ENN++   Q WR+
Sbjct: 363 V-VITNCDYL-RYDDGWMVSSSGTMMNKSSHLVLTANAATSRTNLTGENNVFAAKQAWRI 422

Query: 421 GNYVDPIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCV 480
           GNYV+PI+T+IIG++ MCLEA D +TN+WLE CVKNK+KQ WA+YSD +IRV+NN +LCV
Sbjct: 423 GNYVEPIVTTIIGLRHMCLEATDNDTNVWLESCVKNKTKQYWALYSDDTIRVNNNRNLCV 482

Query: 481 TASSIESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRH 540
           ++S+  S+++IVI RC+G  +QRWVF   GTIS    E++ MDVAQN+V LK+IVL    
Sbjct: 483 SSSTDSSSKLIVIRRCDGSINQRWVFTPQGTISNPGYEAV-MDVAQNDVYLKKIVLSSAT 542

Query: 541 GLVS-QQWTVFY 547
              + QQWTVFY
Sbjct: 543 DKGNGQQWTVFY 547

BLAST of CcUC08G148750.1 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 609.8 bits (1571), Expect = 3.1e-173
Identity = 315/528 (59.66%), Postives = 404/528 (76.52%), Query Frame = 0

Query: 23  ANLHLTKRPVVDVYKSFIQNVRQQFGSKTHKLYGIPVLQHSLSNSDRFYFVDTGNVAGDT 82
           ANL L++      YK+FI  VR++ GS+T++LYGIPVL+HSLSNS+RFY +   +   ++
Sbjct: 4   ANLRLSEAN-SGTYKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQDES 63

Query: 83  ITLAVDAEDMSVVAYL-AGDNDSYFFANAPQLAFDILFSNTLKTILSFNNTFQSIEIAAN 142
           ITLA+D EDM  VAY  AG ++SYFF NAPQ+AF  LF++T + +L+F+NTF+S+E AA 
Sbjct: 64  ITLAIDVEDMVAVAYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENAAG 123

Query: 143 TTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIFQMIFEAAKFKFIEQAVVNSIENGE 202
           TTRQ   LG+   + AISNLF+ +P L P+SFL+I QM+ EA+KF+FIEQ+V  S +N +
Sbjct: 124 TTRQTIVLGVDPLDFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKNEK 183

Query: 203 SFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSVMLYNSRNEVIEVDSIYYPIILPNL 262
           +F P+LAIVSLEDNWSE+SLQIQAS+SLQGLFGS V LYNS NE+IEVDSIYYPIIL N+
Sbjct: 184 TFLPDLAIVSLEDNWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILANV 243

Query: 263 ALQLYHCNTQDYIQMPGVVRPDNSRCYVEERTTWISGRDGLCANVAHALSNDHSLVILFP 322
           ALQLYHC           V   ++ C VE RTT ISGRD LC +VA AL++D S +IL+P
Sbjct: 244 ALQLYHCQ----------VSTGDNECLVETRTTRISGRDALCVDVAGALTSDGSRLILYP 303

Query: 323 CGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAVIYNCSKITKQDATWEVSISGTIM 382
           CG+QVNQ+WTFHSD T+RSLGKCL  + S  G+ L VIY+CSK+  +D +W+VS+ GTIM
Sbjct: 304 CGQQVNQKWTFHSDGTVRSLGKCLATNNSKFGN-LVVIYDCSKLAAEDISWDVSVGGTIM 363

Query: 383 NSAY-DLVLTSNNASKSSNLTMENNIYGGSQGWRVGNYVDPIITSIIGVKQMCLEAKDEN 442
           N  Y DL LTSN A++S+NLTME N Y  SQGWRVGNYV PII SI+G+  MCLEA D N
Sbjct: 364 NPNYEDLALTSNKATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDGN 423

Query: 443 TNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTASS--IESNQVIVILRCNGLASQR 502
           TN+WLEECV N+ +QSWA+YSDG+IRV +N  LCVTASS   ++ +VI IL C+G  +QR
Sbjct: 424 TNMWLEECVPNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSNNQR 483

Query: 503 WVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLVSQQWTVFY 547
           WVF  DG+IS   N+ LAMDVA+++VDLK+I+L+  HG ++QQW +FY
Sbjct: 484 WVFLADGSISTPGNQRLAMDVARSDVDLKKIILHRPHGDLNQQWVLFY 519

BLAST of CcUC08G148750.1 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 363.6 bits (932), Expect = 3.9e-99
Identity = 213/564 (37.77%), Postives = 333/564 (59.04%), Query Frame = 0

Query: 1   MRVLVA-----FIVALCLTTDGIY-----AGNANLHLTKRPVVDVYKSFIQNVRQQFGSK 60
           MRV+ A     +IV L + + GI      + + NL   K      Y+ F+ N+R+   + 
Sbjct: 1   MRVVAAAMLYFYIVVLAICSVGIQGIDYPSVSFNLDGAKSA---TYRDFLSNLRKTVATG 60

Query: 61  THKLYGIPVL--QHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFA 120
           T+++ G+PVL  +  +    RF  V   N  G+T+TLAVD  ++ VVA+ +G+ +SYFF 
Sbjct: 61  TYEVNGLPVLRRESEVQVKSRFVLVPLTNYNGNTVTLAVDVTNLYVVAF-SGNANSYFFK 120

Query: 121 NAPQLAFDILFSNTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHL 180
           +A ++    LF  T +  LSF   + ++E AANT R++  LG    + AI++L+H +   
Sbjct: 121 DATEVQKSNLFVGTKQNTLSFTGNYDNLETAANTRRESIELGPSPLDGAITSLYHGDS-- 180

Query: 181 APVSFLIIFQMIFEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSS 240
              S L++ QM+ EAA+F++IEQ V  S++   SFTPN  ++S+E+NWS +SL+IQ + +
Sbjct: 181 VARSLLVVIQMVSEAARFRYIEQEVRRSLQQATSFTPNALMLSMENNWSSMSLEIQQAGN 240

Query: 241 LQGLFGSSVMLYNSRNEVIEVDSIYYPIILPNLALQLYHCNT---QDYIQMPGVVRPDNS 300
               F  +V L N  +    VD+      +  +A+ L+ C++    + I+MP  +  +++
Sbjct: 241 NVSPFFGTVQLLNYDHTHRLVDNFEELYKITGIAILLFRCSSPSNDNAIRMPLDLAGEDN 300

Query: 301 RCYVEE----RTTW---ISGRDGLCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTI 360
           +    E    RT++   I GRDGLC +V +    D + + L+PCG Q NQ+WTF SD TI
Sbjct: 301 KYNDGETCTLRTSFTRNIVGRDGLCVDVRNGYDTDGTPLQLWPCGTQRNQRWTFDSDDTI 360

Query: 361 RSLGKCLIPSKSISGSPLAVIYNCSKITKQDATWEVSISGTIMNSAYDLVLTSNNASKSS 420
           RS+GKC+  +   +GS + VI+NCS   +    WEV I G+I+N +  LV+T+  A+  +
Sbjct: 361 RSMGKCMTANGLNNGSNI-VIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRT 420

Query: 421 NLTMENNIYGGSQGWRVGNYVDPIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWA 480
            L +E+NIY  SQGW V N V PI+ SI+G K+MCL++  EN  +W+E+C     +Q WA
Sbjct: 421 ILLLEDNIYAASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWA 480

Query: 481 VYSDGSIRVSNNPSLCVTASSIESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMD 540
           +Y D +IRV++   LCVT +   S  +I+IL+C GL SQRW F  DG I   K+  + MD
Sbjct: 481 LYGDRTIRVNSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHV-MD 540

Query: 541 VAQNNVDLKRIVLYPRHGLVSQQW 543
           V  +NV L+ I+++P  G  +QQW
Sbjct: 541 VRASNVSLREIIIFPATGNPNQQW 556

BLAST of CcUC08G148750.1 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 3.6e-97
Identity = 224/574 (39.02%), Postives = 321/574 (55.92%), Query Frame = 0

Query: 1   MRVLVAFIVALCLTTDGIYAGNANLHLTKRPVV-----------DVYKSFIQNVRQQFGS 60
           MRV+   +  + L   G+    A  H    P V           D Y  F++ ++++   
Sbjct: 1   MRVVTKLLYLVVLAICGLGIHGALTHTRVTPPVYPSVSFNLTGADTYGPFLRALQEKVIL 60

Query: 61  KTHKLYGIPVL--QHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFF 120
             H  + +PVL  +  +S+S+RF  V   N +GDT+TLA+D  ++ VVA+ + +  SYFF
Sbjct: 61  GNHTAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVNLYVVAF-SSNGRSYFF 120

Query: 121 ANAPQLAFDILFSNTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPH 180
           + +  +  D LF +T +  L+F   + S+E      R   PLG +S   AIS+L  Y   
Sbjct: 121 SGSTAVQRDNLFVDTTQEELNFTGNYISLERQVGFGRVYIPLGPKSLAQAISSLRTYTLS 180

Query: 181 LAPV-----SFLIIFQMIFEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQ 240
                      L++ QM+ EAA+F++IE  +  SI +   FTP+L ++S+E+NWS +S +
Sbjct: 181 AGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPDLLMLSMENNWSSMSSE 240

Query: 241 IQASSSLQGLFGSSVMLYNSRNEVIEVDSIYYPIILPNLALQLYHC----------NTQD 300
           IQ +    G+F   V L + RN  IEV +      L  +A+ LY C          N  D
Sbjct: 241 IQQAQP-GGIFPGVVQLRDERNNPIEVTNFRRLFELTYIAVLLYGCAPVTSNSYTNNAID 300

Query: 301 --YIQMPGVVRPDNSR--CYVEERTTWISGRDGLCANVAHALSNDHSLVILFPCGEQVNQ 360
              I+MP V R       C V E T  ISG DGLC +V      D + V L PCG + NQ
Sbjct: 301 AQIIKMP-VFRGGGYEKVCSVVEVTRRISGWDGLCVDVRDGHYIDGNTVQLGPCGNECNQ 360

Query: 361 QWTFHSDHTIRSLGKCLIPSKSISGSPLAVIYNCSKITKQDATWEVSISGTIMNSAYDLV 420
            WTF +D TIR LGKCL  S S+      +IY+C+ +  +   W VS  GTI N    LV
Sbjct: 361 LWTFRTDGTIRWLGKCLTTSSSV------MIYDCNTVPPEATKWVVSTDGTITNPRSGLV 420

Query: 421 LTSNNASKSSNLTMENNIYGGSQGWRVGNYVDPIITSIIGVKQMCLEAKDENTNIWLEEC 480
           LT+  A++ + L++ENNI+   QGW VG+ V+P++T I+G KQMCL    EN  +WLE+C
Sbjct: 421 LTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMCLTENGENNFVWLEDC 480

Query: 481 VKNKSKQSWAVYSDGSIRVSNNPSLCVTASSIESNQVIVILRCNGLASQRWVFKVDGTIS 540
           V N+ +Q WA+Y DG+IRV++N SLCVT+   E + +IVIL+C G  +QRWVF  +GTIS
Sbjct: 481 VLNRVEQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTIS 540

Query: 541 PAKNESLAMDVAQNNVDLKRIVLYPRHGLVSQQW 543
              N  L MDVAQ+NV L++I+LYP  G  +QQW
Sbjct: 541 -NPNAKLVMDVAQSNVSLRKIILYPPTGNPNQQW 563

BLAST of CcUC08G148750.1 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 6.2e-97
Identity = 212/529 (40.08%), Postives = 310/529 (58.60%), Query Frame = 0

Query: 34  DVYKSFIQNVRQQFGSKTHKLYGIPVL--QHSLSNSDRFYFVDTGNVAGDTITLAVDAED 93
           D Y+ F++ ++++     H  + +PVL  +  +S+S+RF  V   N +GDT+TLA+D  +
Sbjct: 45  DTYEPFLRALQEKVILGNHTAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVN 104

Query: 94  MSVVAYLAGDNDSYFFANAPQLAFDILFSNTLKTILSFNNTFQSIEIAANTTRQATPLGL 153
           + VVA+ + +  SYFF+ +  +  D LF +T +  L+F   + S+E      R   PLG 
Sbjct: 105 LYVVAF-SSNGKSYFFSGSTAVQRDNLFVDTTQEELNFTGNYTSLERQVGFGRVYIPLGP 164

Query: 154 QSTNSAISNLFHYNPHLAPV-----SFLIIFQMIFEAAKFKFIEQAVVNSIENGESFTPN 213
           +S + AIS+L  Y              L++ QM+ EAA+F++IE  +  SI +   FTP+
Sbjct: 165 KSLDQAISSLRTYTLTAGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPD 224

Query: 214 LAIVSLEDNWSELSLQIQASSSLQGLFGSSVMLYNSRNEVIEVDSIYYPIILPNLALQLY 273
           L ++S+E+NWS +S +IQ +    G+F   V L + RN  IEV +      L  +A+ LY
Sbjct: 225 LLMLSMENNWSSMSSEIQQAQP-GGIFAGVVQLRDERNNSIEVTNFRRLFELTYIAVLLY 284

Query: 274 HC----------NTQD--YIQMPGVVRPDNSR-CYVEERTTWISGRDGLCANVAHALSND 333
            C          N  D   I+MP     +  + C V E T  ISG DGLC +V +    D
Sbjct: 285 GCAPVTSSSYSNNAIDAQIIKMPVFRGGEYEKVCSVVEVTRRISGWDGLCVDVRYGHYID 344

Query: 334 HSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAVIYNCSKITKQDATWE 393
            + V L PCG + NQ WTF +D TIR LGKCL  S S+      +IY+C+ +  +   W 
Sbjct: 345 GNPVQLRPCGNECNQLWTFRTDGTIRWLGKCLTASSSV------MIYDCNTVPPEATKWV 404

Query: 394 VSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRVGNYVDPIITSIIGVKQMC 453
           VSI GTI N    LVLT+  A++ + L++ENNI+   QGW VG+ V+P++T I+G KQMC
Sbjct: 405 VSIDGTITNPHSGLVLTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMC 464

Query: 454 LEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTASSIESNQVIVILRCNG 513
           L    EN  +WLE+CV N+ +Q WA+Y DG+IRV++N SLCVT+   E + +IVIL+C G
Sbjct: 465 LRENGENNFVWLEDCVLNRVQQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEG 524

Query: 514 LASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLVSQQW 543
             +QRWVF  +GTIS   N  L MDVAQ +V L++I+LY   G  +QQW
Sbjct: 525 SGNQRWVFNTNGTIS-NPNAKLLMDVAQRDVSLRKIILYRPTGNPNQQW 563

BLAST of CcUC08G148750.1 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.0e-91
Identity = 210/527 (39.85%), Postives = 306/527 (58.06%), Query Frame = 0

Query: 36  YKSFIQNVRQQFGSKTHKLYGIPVL--QHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMS 95
           Y+ F+Q +R +    +H+ + +PVL  +  +S+SDRF  V   N +   +TLA+D     
Sbjct: 49  YRHFLQELRGKVILGSHRAFDLPVLNPESKVSDSDRFVLVRLTNPSRKKVTLAIDVVTFY 108

Query: 96  VVAYLAGDNDSYFFANAPQLAFDILFSNTLKTILSFNNTFQSIEIAANTTRQATPLGLQS 155
           VVA+   D  SYFF+ + ++  + LF +T +  L+F   + S+E      R   PLG +S
Sbjct: 109 VVAFAQNDR-SYFFSGSSEVQRENLFVDTTQEDLNFKGDYTSLEHQVGFGRVYIPLGPKS 168

Query: 156 TNSAISNLFHY------NPHLAPVSFLIIFQMIFEAAKFKFIEQAVVNSIENGESFTPNL 215
              +IS+L  Y      N  LA  S L++ QM+ EAA+F++I+  +  SI + + FTP+L
Sbjct: 169 LAQSISSLSTYKSSAGDNKRLAR-SLLVVIQMVSEAARFRYIQLRIQASITDAKEFTPDL 228

Query: 216 AIVSLEDNWSELSLQIQASSSLQGLFGSSVMLYNSRNEVIEVDSIYYPIILPNLALQLYH 275
            ++S+E+ WS +S +IQ +    G F   V L + RN  I+V +      L ++A+ L+ 
Sbjct: 229 LMLSMENKWSSMSSEIQQAQP-GGAFAQVVKLLDQRNHPIDVTNFRRLFQLTSVAVLLHG 288

Query: 276 CNTQDYIQMPGVV--------RPDNSRC-YVEERTTWISGRDGLCANVAHALSNDHSLVI 335
           C T    +MP  +          D  RC  VEE T  I GRDG CA V +    D + V 
Sbjct: 289 CPT--VTKMPAYIIKMPVFNGGEDEERCSVVEEVTRRIGGRDGFCAEVKNGDEKDGTPVQ 348

Query: 336 LFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAVIYNCSKITKQDATWEVSISG 395
           L  CGEQ NQQWTF +D TI+SLGKCL  S S+      +IYNC  +  +   W VSI G
Sbjct: 349 LSSCGEQSNQQWTFSTDGTIQSLGKCLTTSSSV------MIYNCKVVPPESTKWVVSIDG 408

Query: 396 TIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRVGNYVDPIITSIIGVKQMCLEAKD 455
           TI N    LVLT+  A++ + +++E N++   QGW VGN V+P++T I+G +QMCLE   
Sbjct: 409 TITNPRSGLVLTAPKAAEGTLVSLEKNVHAARQGWIVGN-VEPLVTFIVGYEQMCLETNP 468

Query: 456 ENTNIWLEEC-VKNKSK--QSWAVYSDGSIRVSNNPSLCVTASSIESNQVIVILRCNGLA 515
            N ++ L +C VK+ SK  Q WA+Y DG+IRV+N+ SLCVT+    SN+ I+IL+C G A
Sbjct: 469 GNNDVSLGDCSVKSASKVDQKWALYGDGTIRVNNDRSLCVTSEGKSSNEPIIILKCLGWA 528

Query: 516 SQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLVSQQW 543
           +QRWVF  DGTIS   +  L M V QN+V L++I+L    G  +QQW
Sbjct: 529 NQRWVFNTDGTIS-NPDSKLVMHVDQNDVPLRKIILSHPSGTSNQQW 562

BLAST of CcUC08G148750.1 vs. ExPASy TrEMBL
Match: A0A0A0KN71 (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 8.4e-222
Identity = 393/548 (71.72%), Postives = 458/548 (83.58%), Query Frame = 0

Query: 1   MRVLVAFIVALCLTTDGIYAGNANLHLTKRPVVDVYKSFIQNVRQQFGSKTHKLYGIPVL 60
           MRVL+AFIV L + T+          L+KRPVVDVY+S+IQ +RQ+FGSKTHKLYGIPVL
Sbjct: 1   MRVLLAFIVTLSIATNA---------LSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVL 60

Query: 61  QHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQLAFDILFS 120
            HSLSNSDRF+ +D GN  GDTIT AVDA+DMSVVAYLAGDNDSYFF+NAP+ AFDILF 
Sbjct: 61  HHSLSNSDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFP 120

Query: 121 NTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIFQMI 180
            T + +L+F+N+F+SIEIAANTTR+ATPLGL+ +N+AI+NLFHY+P LAPVSFLI+FQM+
Sbjct: 121 KTNQNLLNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMV 180

Query: 181 FEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSVMLY 240
           FE+AKFKFIEQ +VNSI NGE+FTP+LA++SLEDNWSELSL+IQAS+SLQGLFGSSV LY
Sbjct: 181 FESAKFKFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLY 240

Query: 241 NSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIQMPGVVRPD--NSRCYVEERTTWISG 300
           NS+NE +EVDSIYYP+IL NLALQLYHCN QDYI+MP     D  N RCYV+ERT  ISG
Sbjct: 241 NSKNEAVEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISG 300

Query: 301 RDGLCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAV 360
           +DGLCA+VA     D S VI  PCG+Q NQQWTFH DHTIRS  KCLIP+KS   +PLAV
Sbjct: 301 QDGLCADVA----RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKS-KANPLAV 360

Query: 361 IYNCSKITKQDATWEVSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRVGNY 420
           I NC+K++++DATW+VSISGTIMN AYDLVLTS N    ++L+M+ N Y G+QGWRVGNY
Sbjct: 361 IQNCNKVSQEDATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNY 420

Query: 421 VDPIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTAS 480
           V+PII SIIGVKQMCLEA +ENTNIWLEECVKNK +QSWAV+SDGSIRV+N+ SLC+TAS
Sbjct: 421 VEPIIASIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTAS 480

Query: 481 SIESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLV 540
           SIES Q IVI +CNGLASQRWV K DGTIS  K E L MDVAQ+NVDLK IVLYPR  LV
Sbjct: 481 SIESKQRIVIAKCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLV 534

Query: 541 SQQWTVFY 547
           SQ W   Y
Sbjct: 541 SQHWVALY 534

BLAST of CcUC08G148750.1 vs. ExPASy TrEMBL
Match: A0A5A7V3Q8 (rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G00010 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.5e-218
Identity = 390/548 (71.17%), Postives = 456/548 (83.21%), Query Frame = 0

Query: 1   MRVLVAFIVALCLTTDGIYAGNANLHLTKRPVVDVYKSFIQNVRQQFGSKTHKLYGIPVL 60
           MRVL+AF+V L   T+          L+KRPVVDVY+S+IQ +RQ+FGSKTHKLYGIPVL
Sbjct: 1   MRVLLAFVVTLSFATNA---------LSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVL 60

Query: 61  QHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQLAFDILFS 120
           QHSLSNSDRF+ +DTGN  GDTITLAVDA+DMSVVAYLAG++DSYFF+NAP+ AFDILF 
Sbjct: 61  QHSLSNSDRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFP 120

Query: 121 NTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIFQMI 180
           NT + +L+F+++F+SIE AANTTRQATPLG + +N+AI+NLFHY+  LAPVSFLI+FQMI
Sbjct: 121 NTNQNLLNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMI 180

Query: 181 FEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSVMLY 240
           FE+AKF FIEQ +VNSI+ GE+FTP+LA++SLEDNWSELSLQIQAS+SLQGLFGSSV LY
Sbjct: 181 FESAKFNFIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLY 240

Query: 241 NSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIQMPGVVRPD--NSRCYVEERTTWISG 300
           NS+NE IEVDSIYYP+IL NLALQLYHCN QDYI+MP     D  N RCYV+ERT  ISG
Sbjct: 241 NSKNEAIEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISG 300

Query: 301 RDGLCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAV 360
           RDGLC +VA     D S VI  PCG+Q NQ+WTF+ DHTIRS  KCLIP+KS   +P AV
Sbjct: 301 RDGLCVDVA----RDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKS-KANPFAV 360

Query: 361 IYNCSKITKQDATWEVSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRVGNY 420
           I +C+K+++QDATW+VSISGTIMN AYDLVLTSNN    ++L+M+ N Y G+QGWRVGN+
Sbjct: 361 ILDCNKVSQQDATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNF 420

Query: 421 VDPIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTAS 480
           V+P+I SIIGVKQMCLEA +ENTNIWLEECVKNK +QSWAVYSDGSIRV+N+ SLCVTAS
Sbjct: 421 VEPLIVSIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTAS 480

Query: 481 SIESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLV 540
           SIE  Q IVI +CNGLASQRWV   DGTIS  KNE L MDVAQ+NVDLK IVLYPR  LV
Sbjct: 481 SIEPKQRIVIGKCNGLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLV 534

Query: 541 SQQWTVFY 547
           SQQW   Y
Sbjct: 541 SQQWVALY 534

BLAST of CcUC08G148750.1 vs. ExPASy TrEMBL
Match: A0A1S3CIM5 (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103501401 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.5e-218
Identity = 390/548 (71.17%), Postives = 456/548 (83.21%), Query Frame = 0

Query: 1   MRVLVAFIVALCLTTDGIYAGNANLHLTKRPVVDVYKSFIQNVRQQFGSKTHKLYGIPVL 60
           MRVL+AF+V L   T+          L+KRPVVDVY+S+IQ +RQ+FGSKTHKLYGIPVL
Sbjct: 1   MRVLLAFVVTLSFATNA---------LSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVL 60

Query: 61  QHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQLAFDILFS 120
           QHSLSNSDRF+ +DTGN  GDTITLAVDA+DMSVVAYLAG++DSYFF+NAP+ AFDILF 
Sbjct: 61  QHSLSNSDRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFP 120

Query: 121 NTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIFQMI 180
           NT + +L+F+++F+SIE AANTTRQATPLG + +N+AI+NLFHY+  LAPVSFLI+FQMI
Sbjct: 121 NTNQNLLNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMI 180

Query: 181 FEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSVMLY 240
           FE+AKF FIEQ +VNSI+ GE+FTP+LA++SLEDNWSELSLQIQAS+SLQGLFGSSV LY
Sbjct: 181 FESAKFNFIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLY 240

Query: 241 NSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIQMPGVVRPD--NSRCYVEERTTWISG 300
           NS+NE IEVDSIYYP+IL NLALQLYHCN QDYI+MP     D  N RCYV+ERT  ISG
Sbjct: 241 NSKNEAIEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISG 300

Query: 301 RDGLCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAV 360
           RDGLC +VA     D S VI  PCG+Q NQ+WTF+ DHTIRS  KCLIP+KS   +P AV
Sbjct: 301 RDGLCVDVA----RDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKS-KANPFAV 360

Query: 361 IYNCSKITKQDATWEVSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRVGNY 420
           I +C+K+++QDATW+VSISGTIMN AYDLVLTSNN    ++L+M+ N Y G+QGWRVGN+
Sbjct: 361 ILDCNKVSQQDATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNF 420

Query: 421 VDPIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTAS 480
           V+P+I SIIGVKQMCLEA +ENTNIWLEECVKNK +QSWAVYSDGSIRV+N+ SLCVTAS
Sbjct: 421 VEPLIVSIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTAS 480

Query: 481 SIESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLV 540
           SIE  Q IVI +CNGLASQRWV   DGTIS  KNE L MDVAQ+NVDLK IVLYPR  LV
Sbjct: 481 SIEPKQRIVIGKCNGLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLV 534

Query: 541 SQQWTVFY 547
           SQQW   Y
Sbjct: 541 SQQWVALY 534

BLAST of CcUC08G148750.1 vs. ExPASy TrEMBL
Match: B7X8M2 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MCL1 PE=1 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 9.4e-149
Identity = 293/552 (53.08%), Postives = 389/552 (70.47%), Query Frame = 0

Query: 1   MRVLVAFI-VALCLTTDGIYAGNANLHLTKRPV-VDVYKSFIQNVRQQFG-SKTHKLYGI 60
           MRVL  +I VAL LT +GI     NL L++     D YKSFI+N+R+Q     ++   GI
Sbjct: 3   MRVLAVYIVVALSLTINGI---ECNLSLSQSNFSADTYKSFIKNLRKQLTIGASYGSAGI 62

Query: 61  PVLQHSLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQLAFDI 120
           P+L+HS+   +RF  VD  N   +TITLA++ ED    AY A D  SYFF NAP +A  +
Sbjct: 63  PILKHSVPICERFLLVDLTNGDNETITLAINVEDAGFAAYRAADR-SYFFQNAPPIASYV 122

Query: 121 LFSNTLKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIF 180
           +F++T + I++FNNTF+SIEI   TTR  TPLG+    ++I +LF ++ +  P SFL++ 
Sbjct: 123 IFTDTNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVPTSFLVLI 182

Query: 181 QMIFEAAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSV 240
           QM+ EAAKFKFIEQ V++SI + E FTP LA++SLE+NW++LSLQ+QAS SL G+FG SV
Sbjct: 183 QMVLEAAKFKFIEQKVIHSIMDMEDFTPGLAMLSLEENWTQLSLQLQASESLNGVFGDSV 242

Query: 241 MLYNSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIQMPGVVRP--DNSRCYVEERTTW 300
            LYNS +E I VDS+YYPI+  N+A QLY C       MP +  P  +N +C  ++RTT 
Sbjct: 243 SLYNSMDEPIGVDSMYYPILTANMAFQLYQCPYGVIRMMPTITMPNQNNEQCSPQQRTTR 302

Query: 301 ISGRDGLCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSP 360
           ISGRDGLC +V  AL+ D S VIL+PCG+Q NQQWTF+ D+TIRSLGKCL  S   SGS 
Sbjct: 303 ISGRDGLCVDVYGALTADGSRVILYPCGQQQNQQWTFYPDNTIRSLGKCLATSALSSGSN 362

Query: 361 LAVIYNCSKITKQDATWEVSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRV 420
           + VI NC  + + D  W VS SGT+MN +  LVLT+N A+  +NLT ENN++   Q WR+
Sbjct: 363 V-VITNCDYL-RYDDGWMVSSSGTMMNKSSHLVLTANAATSRTNLTGENNVFAAKQAWRI 422

Query: 421 GNYVDPIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCV 480
           GNYV+PI+T+IIG++ MCLEA D +TN+WLE CVKNK+KQ WA+YSD +IRV+NN +LCV
Sbjct: 423 GNYVEPIVTTIIGLRHMCLEATDNDTNVWLESCVKNKTKQYWALYSDDTIRVNNNRNLCV 482

Query: 481 TASSIESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRH 540
           ++S+  S+++IVI RC+G  +QRWVF   GTIS    E++ MDVAQN+V LK+IVL    
Sbjct: 483 SSSTDSSSKLIVIRRCDGSINQRWVFTPQGTISNPGYEAV-MDVAQNDVYLKKIVLSSAT 542

Query: 541 GLVS-QQWTVFY 547
              + QQWTVFY
Sbjct: 543 DKGNGQQWTVFY 547

BLAST of CcUC08G148750.1 vs. ExPASy TrEMBL
Match: A0A0A0K979 (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 1.0e-134
Identity = 263/546 (48.17%), Postives = 369/546 (67.58%), Query Frame = 0

Query: 3   VLVAFIVALCLTTDGIYAGNANLHLTKRPVVDVYKSFIQNVRQQFGSKTHKLYGIPVLQH 62
           +++AF+     + DG     ++  L    VVD YK+FI  +RQ+    T KLY IP+L+ 
Sbjct: 8   IIIAFLSLATPSIDGNQLSLSSSDL----VVDGYKNFINVIRQRLTENTLKLYDIPILES 67

Query: 63  SLSNSDRFYFVDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQLAFDILFSNT 122
           SL  + RF  ++ GN   +TI+LA+D  ++ VV Y   +N+SY F +AP+ A D++F  T
Sbjct: 68  SLPPTQRFMNINIGNQNDETISLAIDRVNLGVVGY-RSNNNSYIFLDAPKAALDVVFPET 127

Query: 123 LKTILSFNNTFQSIEIAANTTRQATPLGLQSTNSAISNLFHYNPHLAPVSFLIIFQMIFE 182
            + +L FN+ ++SIE A+ TTR  T LGL+  NSAISNLFHY     P SFL+I QM+ E
Sbjct: 128 CRVVLGFNSDYESIEKASGTTRLQTLLGLEPLNSAISNLFHYRRADIPGSFLVILQMVLE 187

Query: 183 AAKFKFIEQAVVNSIENGESFTPNLAIVSLEDNWSELSLQIQASSSLQGLFGSSVMLYNS 242
            +KFKFIEQ+V+ S++ G +F P LAIVSLEDNW +LS QIQAS SLQGLFG ++ LY+S
Sbjct: 188 GSKFKFIEQSVIQSLKYGYNFKPGLAIVSLEDNWDKLSSQIQASPSLQGLFGEAIRLYDS 247

Query: 243 RNEVIEVDSIYYPIILPNLALQLYHCN-TQDYIQMPGVVRPDNSRCYVEERTTWISGRDG 302
            ++ I+VDSIYY II  N+A QL+HCN + ++I+MP  V   +  C V+ RT  ISG++G
Sbjct: 248 NDKFIDVDSIYYVIITTNIAFQLHHCNVSTNFIRMPSDV---DDSCNVQTRTAMISGQNG 307

Query: 303 LCANVAHALSNDHSLVILFPCGEQVNQQWTFHSDHTIRSLGKCLIPSKSISGSPLAVIYN 362
            C + +  L  + + +IL+ C  Q+NQ+WTF SD TIR   KCL    +   S   V+YN
Sbjct: 308 FCVDSSRILDYNGNPIILYRCINQLNQEWTFLSDKTIRYSNKCL----TFETSRYVVLYN 367

Query: 363 CSKI-TKQDATWEVSISGTIMNSAYDLVLTSNNASKSSNLTMENNIYGGSQGWRVGNYVD 422
           CS++  K +  W V+I GTI N +  LVLT++ ++  S L +E N +  SQGWRVGNYV 
Sbjct: 368 CSEVEEKGNIRWNVAIDGTISNPSSGLVLTTDPSTNRSQLIVEVNKFTTSQGWRVGNYVK 427

Query: 423 PIITSIIGVKQMCLEAKDENTNIWLEECVKNKSKQSWAVYSDGSIRVSNNPSLCVTASSI 482
           PII SIIG+++MCLEA + NTN+WLE+CVKNK++Q WAVYSDGSIRV+   +LCV++SS 
Sbjct: 428 PIIGSIIGMEEMCLEATNNNTNMWLEKCVKNKAEQYWAVYSDGSIRVNRKRNLCVSSSSN 487

Query: 483 ESNQVIVILRCNGLASQRWVFKVDGTISPAKNESLAMDVAQNNVDLKRIVLYPRHGLVSQ 542
            S  +++I  C G ++QRW F  +GTI   + + + +DV  + V  KRI+LYP+ GL +Q
Sbjct: 488 RSGALLIIDECKGTSNQRWNFLANGTILNPETKKV-VDVYGSMVSAKRIILYPKTGLANQ 540

Query: 543 QWTVFY 547
           QWT+FY
Sbjct: 548 QWTLFY 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884532.13.0e-22674.95seed lectin-like [Benincasa hispida][more]
XP_004140357.11.7e-22171.72seed lectin [Cucumis sativus] >KGN51070.1 hypothetical protein Csa_008446 [Cucum... [more]
XP_008463192.13.0e-21871.17PREDICTED: nigrin b-like [Cucumis melo] >KAA0062872.1 nigrin b-like [Cucumis mel... [more]
U3KRF8.22.4e-17059.66RecName: Full=Seed lectin; Short=SGSL; Contains: RecName: Full=Seed lectin Aalph... [more]
XP_022156703.11.9e-14853.08seed lectin-like [Momordica charantia] >BAH05018.1 galactose-binding type-2 ribo... [more]
Match NameE-valueIdentityDescription
U3KRF83.1e-17359.66Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2[more]
P331833.9e-9937.77Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2[more]
O224153.6e-9739.02Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=... [more]
Q413586.2e-9740.08Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1[more]
P935431.0e-9139.85Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KN718.4e-22271.72rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1[more]
A0A5A7V3Q81.5e-21871.17rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G0... [more]
A0A1S3CIM51.5e-21871.17rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103501401 PE=3 SV=1[more]
B7X8M29.4e-14953.08rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MCL1 PE=1 SV=1[more]
A0A0A0K9791.0e-13448.17rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 82..97
score: 41.61
coord: 144..158
score: 38.77
coord: 207..224
score: 45.03
coord: 34..47
score: 29.51
coord: 173..193
score: 45.61
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 420..545
e-value: 1.8E-21
score: 87.3
coord: 292..416
e-value: 1.4E-12
score: 57.8
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 426..542
e-value: 1.4E-19
score: 70.6
coord: 297..413
e-value: 1.3E-13
score: 51.3
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 419..545
score: 32.066998
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 290..416
score: 21.8925
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 426..543
e-value: 3.16153E-19
score: 81.783
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 296..413
e-value: 1.6198E-12
score: 62.523
NoneNo IPR availableGENE3D2.80.10.50coord: 285..420
e-value: 7.2E-26
score: 93.0
NoneNo IPR availableGENE3D2.80.10.50coord: 421..546
e-value: 1.4E-39
score: 136.6
NoneNo IPR availableGENE3D6.10.250.2640coord: 23..64
e-value: 2.9E-15
score: 57.6
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 372..544
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 27..373
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 34..223
e-value: 8.9E-27
score: 94.0
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 372..544
coord: 27..373
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 187..272
e-value: 5.2E-25
score: 89.2
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 68..186
e-value: 1.7E-23
score: 85.0
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 34..272
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 287..416
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 422..544

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CcUC08G148750CcUC08G148750gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC08G148750.1-exonCcUC08G148750.1-exon-CicolChr08:13435485..13437125exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC08G148750.1-cdsCcUC08G148750.1-cds-CicolChr08:13435485..13437125CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CcUC08G148750.1CcUC08G148750.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017148 negative regulation of translation
biological_process GO:0006952 defense response
biological_process GO:0035821 modulation of process of other organism
molecular_function GO:0030598 rRNA N-glycosylase activity
molecular_function GO:0090729 toxin activity