CcUC03G045000.1 (mRNA) Watermelon (PI 537277) v1

Overview
NameCcUC03G045000.1
TypemRNA
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionHigh-affinity nitrate transporter 2.2
LocationCicolChr03: 2969658 .. 2971669 (+)
Sequence length1593
RNA-Seq ExpressionCcUC03G045000.1
SyntenyCcUC03G045000.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGATGTTGAAGGTTCCCCAGGAAGCTCTATGCATGGGGTGACCGGAAGAGAGCAAAGTTTTGCATTTTCCGTAGCTTCCCCAATTGTCCCAACGGACACAACAGCGAAATTTGCATTACCCGTTGACTCTGAGCACAAAGCGAAGGTTTTCAAGGTGTGGTCTTTAGCCAACCCCCACATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTTACATGCTTCGTCTCCACATTCGCAGCCGCCCCTCTGGTCCCCATCATGCGAGACAATCTCAACCTTACCAAAGTTGACATCGGTCACGCCGGAGTCACCTCCGTGTCTGGCAGTATCTTCTCTCGTCTTGTCATGGGGGCTGTCTGTGACCCTCTCGGGCCACGCTATGGGTGTGCTTTTCTCATTATGCTCTCCGCCCCCACCGTGTCTTGTATGTCCTTTGTGTCCAACGCTGCTGGGTATATAGCTGTGAGCTTCATGATTGGCTTCTCGCTTGCCACCTTCGTCTCCTGCCAGTATTGGATGAGCACCATGTTCAACAGTCAGATCATTGGGCTTGTGAATGGGACTGCTGCTGGGTGGGGGAATATGGGCGGAGGAGCCACTCAGCTCATCATGCCTTTGTTGTACGATGTCATTCAGAGAGCTGGCGCTACTCCTTTCACTGCTTGGAGGATTGCCTTCTTTACCCCTGGTTCCCTTCATATTCCCATGGGCATTTTGGTCTTAACTCTTGGCCAGGACTTGCCTGATGGTAACCTTACCAGTCTTCAGAAGAAGGGCAATGTTGCCAACGATACCTTCTCCAATGTAATTACTCCCTTCCTTCTCCATCTATATACCTATATATCTCTTCTCAATTCATTCTTAAATTTTACTTTAATTATACTCCTCTCTCCAATATAATATAATATCTCCTCACAGATCCTGTGGTATGCTGTCACCAATTACCGAACTTGGCTCTTCGTACTCCTCTATCGCTTCTCCATTGGGGTCGAACTTTCCCACGATAACGTCATTGCCGAATACTGCTTCGACAGGTAAAATAAGCAAATCTGATTTTTTAACCTAATATCAAACCCCGTCAGTCAATCCTAATCCTATGCATATGTGTTATGCAATCAACAATCATAGAAAGTGATCACAAGACGAAAACAATATTATGAAAAACCCACTTATCCGTGTGTTGCCCAAGTCCACGTACAAATGGAGACACCATTTACATTAACACAGAAATATCTAGGTTTTAGTTTCTCCACCAAGGGCGCGTTTTTTTACGATTCCTATAACATATTCAAAGCCTTACGACCTTACGTTTTGAATTTGTGTTTGTCATATTTAACAGGTTAGATCTAAAGCTACACACGGCAGGAATTATCGCAGCAACGTTCGGGATGGTGAATCTAGTGGCTCGACCGTTCGGGGGGTATGCATCAGACGTGGCAGCGCGGTACTTCGGGATGCGAGGAAGGCTGTGGACTTTGTGGATCCTACAAACATGTGGGGGTGTGTTTTGCATGTGGCTTGGGAAAGCAAACAAACGGCCAACAGCGATAGTTGCAATGATATTATTCTCAATAGGAGCACAGGCTGCATGTGGGGCAACATTTGGGATCATTCCCTTCAGTTCTCGACGGTCTTTAGGAATTATATCTGGACTGACAGGAGCAGGAGGCAACTTTGGAAGGGGTTTGACACAATTAGTGTTCTTCTCAACGACCAAATACTCAACCGTCACATGCTTTTTCTTTATGGGAATAATGATTGTATGTTGCACTGTACCAGTCAGTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTTTACCTCCCACGAAACATGTTGAAAAATCCACTGAGGCATTTTATTATGGCTCTGAATGGACTGAGGATGAAAAGAAGAAGGGTTTGCATCAACAAAGCATTAAGTGTGCTGAGAATAGCAAATCTGAACGTGGTAGGCGTGTGGCTTCAGCGCCAACTCCACCAAATATAACTCCTTCCCATGTTTGA

mRNA sequence

ATGGGAGATGTTGAAGGTTCCCCAGGAAGCTCTATGCATGGGGTGACCGGAAGAGAGCAAAGTTTTGCATTTTCCGTAGCTTCCCCAATTGTCCCAACGGACACAACAGCGAAATTTGCATTACCCGTTGACTCTGAGCACAAAGCGAAGGTTTTCAAGGTGTGGTCTTTAGCCAACCCCCACATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTTACATGCTTCGTCTCCACATTCGCAGCCGCCCCTCTGGTCCCCATCATGCGAGACAATCTCAACCTTACCAAAGTTGACATCGGTCACGCCGGAGTCACCTCCGTGTCTGGCAGTATCTTCTCTCGTCTTGTCATGGGGGCTGTCTGTGACCCTCTCGGGCCACGCTATGGGTGTGCTTTTCTCATTATGCTCTCCGCCCCCACCGTGTCTTGTATGTCCTTTGTGTCCAACGCTGCTGGGTATATAGCTGTGAGCTTCATGATTGGCTTCTCGCTTGCCACCTTCGTCTCCTGCCAGTATTGGATGAGCACCATGTTCAACAGTCAGATCATTGGGCTTGTGAATGGGACTGCTGCTGGGTGGGGGAATATGGGCGGAGGAGCCACTCAGCTCATCATGCCTTTGTTGTACGATGTCATTCAGAGAGCTGGCGCTACTCCTTTCACTGCTTGGAGGATTGCCTTCTTTACCCCTGGTTCCCTTCATATTCCCATGGGCATTTTGGTCTTAACTCTTGGCCAGGACTTGCCTGATGGTAACCTTACCAGTCTTCAGAAGAAGGGCAATGTTGCCAACGATACCTTCTCCAATATCCTGTGGTATGCTGTCACCAATTACCGAACTTGGCTCTTCGTACTCCTCTATCGCTTCTCCATTGGGGTCGAACTTTCCCACGATAACGTCATTGCCGAATACTGCTTCGACAGGTTAGATCTAAAGCTACACACGGCAGGAATTATCGCAGCAACGTTCGGGATGGTGAATCTAGTGGCTCGACCGTTCGGGGGGTATGCATCAGACGTGGCAGCGCGGTACTTCGGGATGCGAGGAAGGCTGTGGACTTTGTGGATCCTACAAACATGTGGGGGTGTGTTTTGCATGTGGCTTGGGAAAGCAAACAAACGGCCAACAGCGATAGTTGCAATGATATTATTCTCAATAGGAGCACAGGCTGCATGTGGGGCAACATTTGGGATCATTCCCTTCAGTTCTCGACGGTCTTTAGGAATTATATCTGGACTGACAGGAGCAGGAGGCAACTTTGGAAGGGGTTTGACACAATTAGTGTTCTTCTCAACGACCAAATACTCAACCGTCACATGCTTTTTCTTTATGGGAATAATGATTGTATGTTGCACTGTACCAGTCAGTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTTTACCTCCCACGAAACATGTTGAAAAATCCACTGAGGCATTTTATTATGGCTCTGAATGGACTGAGGATGAAAAGAAGAAGGGTTTGCATCAACAAAGCATTAAGTGTGCTGAGAATAGCAAATCTGAACGTGGTAGGCGTGTGGCTTCAGCGCCAACTCCACCAAATATAACTCCTTCCCATGTTTGA

Coding sequence (CDS)

ATGGGAGATGTTGAAGGTTCCCCAGGAAGCTCTATGCATGGGGTGACCGGAAGAGAGCAAAGTTTTGCATTTTCCGTAGCTTCCCCAATTGTCCCAACGGACACAACAGCGAAATTTGCATTACCCGTTGACTCTGAGCACAAAGCGAAGGTTTTCAAGGTGTGGTCTTTAGCCAACCCCCACATGAGAACCTTCCACCTTTCTTGGATTTCCTTCTTTACATGCTTCGTCTCCACATTCGCAGCCGCCCCTCTGGTCCCCATCATGCGAGACAATCTCAACCTTACCAAAGTTGACATCGGTCACGCCGGAGTCACCTCCGTGTCTGGCAGTATCTTCTCTCGTCTTGTCATGGGGGCTGTCTGTGACCCTCTCGGGCCACGCTATGGGTGTGCTTTTCTCATTATGCTCTCCGCCCCCACCGTGTCTTGTATGTCCTTTGTGTCCAACGCTGCTGGGTATATAGCTGTGAGCTTCATGATTGGCTTCTCGCTTGCCACCTTCGTCTCCTGCCAGTATTGGATGAGCACCATGTTCAACAGTCAGATCATTGGGCTTGTGAATGGGACTGCTGCTGGGTGGGGGAATATGGGCGGAGGAGCCACTCAGCTCATCATGCCTTTGTTGTACGATGTCATTCAGAGAGCTGGCGCTACTCCTTTCACTGCTTGGAGGATTGCCTTCTTTACCCCTGGTTCCCTTCATATTCCCATGGGCATTTTGGTCTTAACTCTTGGCCAGGACTTGCCTGATGGTAACCTTACCAGTCTTCAGAAGAAGGGCAATGTTGCCAACGATACCTTCTCCAATATCCTGTGGTATGCTGTCACCAATTACCGAACTTGGCTCTTCGTACTCCTCTATCGCTTCTCCATTGGGGTCGAACTTTCCCACGATAACGTCATTGCCGAATACTGCTTCGACAGGTTAGATCTAAAGCTACACACGGCAGGAATTATCGCAGCAACGTTCGGGATGGTGAATCTAGTGGCTCGACCGTTCGGGGGGTATGCATCAGACGTGGCAGCGCGGTACTTCGGGATGCGAGGAAGGCTGTGGACTTTGTGGATCCTACAAACATGTGGGGGTGTGTTTTGCATGTGGCTTGGGAAAGCAAACAAACGGCCAACAGCGATAGTTGCAATGATATTATTCTCAATAGGAGCACAGGCTGCATGTGGGGCAACATTTGGGATCATTCCCTTCAGTTCTCGACGGTCTTTAGGAATTATATCTGGACTGACAGGAGCAGGAGGCAACTTTGGAAGGGGTTTGACACAATTAGTGTTCTTCTCAACGACCAAATACTCAACCGTCACATGCTTTTTCTTTATGGGAATAATGATTGTATGTTGCACTGTACCAGTCAGTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTTTACCTCCCACGAAACATGTTGAAAAATCCACTGAGGCATTTTATTATGGCTCTGAATGGACTGAGGATGAAAAGAAGAAGGGTTTGCATCAACAAAGCATTAAGTGTGCTGAGAATAGCAAATCTGAACGTGGTAGGCGTGTGGCTTCAGCGCCAACTCCACCAAATATAACTCCTTCCCATGTTTGA

Protein sequence

MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPHMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGAVCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGILVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDNVIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGNFGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTEAFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV
Homology
BLAST of CcUC03G045000.1 vs. NCBI nr
Match: XP_038895485.1 (high affinity nitrate transporter 2.4-like [Benincasa hispida] >XP_038895611.1 high affinity nitrate transporter 2.4-like [Benincasa hispida])

HSP 1 Score: 981.9 bits (2537), Expect = 2.2e-282
Identity = 491/530 (92.64%), Postives = 505/530 (95.28%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKF+LPVDSEHKAKVFK+WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFSLPVDSEHKAKVFKLWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPI+RDNLNLTKVDIG+AGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFLIMLSAPTV CMSFVSNAAGYIAV FMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFF PG LH+ MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGNL SLQKKGNV  D FSN+LWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPDGNLGSLQKKGNVTKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY +DR DLKLHTAGIIAATFGM NLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GGVFCMWLGKANK PTAIVAMILFSIGAQAACGATFGIIPF SRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFSTTKYST T  FFMGIMIVCCT+PV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFFMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            FYYGSEWTEDEKKKGLHQQSIK AENS+SERGRRVASAPTPPN TPSHV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 530

BLAST of CcUC03G045000.1 vs. NCBI nr
Match: XP_031736393.1 (high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP53714.1 high affinity nitrate transporter 2.1 [Cucumis sativus] >KAE8652752.1 hypothetical protein Csa_014056 [Cucumis sativus])

HSP 1 Score: 973.8 bits (2516), Expect = 6.1e-280
Identity = 486/530 (91.70%), Postives = 502/530 (94.72%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPI+RDNLNLTKVDIG+AGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFLIMLSAPTV CMSFVSNAAGYIAV FMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFF PG LH+ MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGNL SLQKKGNVA D FSN+LWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY +DR DLKLHTAGIIAATFGM NLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA K P AI AMILFSIGAQAACGATFGIIPF SRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFSTTKYST T  F MGIMIVCCT+PV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            FYYGSEWTEDEKKKGLHQQSIK AENS+SERGRRVASAPTPPN TP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CcUC03G045000.1 vs. NCBI nr
Match: NP_001274401.1 (high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-affinity nitrate transporter [Cucumis sativus])

HSP 1 Score: 971.8 bits (2511), Expect = 2.3e-279
Identity = 486/530 (91.70%), Postives = 501/530 (94.53%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPI+RDNLNLTKVDIG+AGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFLIMLSAPTV CMSFVSNAAGYIAV FMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFF PG LH+ MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGNL SLQKKGNVA D FSN+LWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY +DR DLKLHTAGIIAATFGM NLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA K P AI AMILFSIGAQAACGATFGIIPF SRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFSTTKYST T  F MGIMIVCCT+PV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            FYYGSEWTEDEKKKGLHQQSIK AENS+SERGRRVASAPTPPN TPS V
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

BLAST of CcUC03G045000.1 vs. NCBI nr
Match: AGO64298.1 (high-affinity nitrate transporter NRT2.2 [Cucumis sativus])

HSP 1 Score: 971.5 bits (2510), Expect = 3.0e-279
Identity = 484/530 (91.32%), Postives = 502/530 (94.72%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPI+RDNLNLTKVDIG+AGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFLIMLSAPTV CMSFVS+AAGYIAV FMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFF PG LH+ MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGNL SLQKKGNVA D FSN+LWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY +DR DLKLHTAGIIAATFGM NLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA K P AI AMILFSIGAQAACGATFGIIPF SRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFSTTKYST T  F MGIMIVCCT+PV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            FYYGSEWTEDEKKKGLHQQSIK AENS+SERGRRVASAPTPPN TP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CcUC03G045000.1 vs. NCBI nr
Match: NP_001269063.2 (high affinity nitrate transporter 2.4-like [Cucumis sativus])

HSP 1 Score: 967.6 bits (2500), Expect = 4.3e-278
Identity = 483/530 (91.13%), Postives = 500/530 (94.34%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPI+RDNLNLTKVDIG+AGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFLIMLSAPTV CMSFVS+AAGYIAV FMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFF PG LH+ MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGNL SLQKKGNVA D FSN+LWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY +DR DLKLHTAGIIAATFGM NLVARPFGGYASDVAARYFGMRGRLW LWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWALWILQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA K P AI AMILFSIGAQAACGATFGIIPF SRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFSTTKYST T  F MGIMIVCCT+PV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            FYYGSEWTEDEKKKGLHQQSIK AENS+SERGRRVASAPTPPN TPS V
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

BLAST of CcUC03G045000.1 vs. ExPASy Swiss-Prot
Match: O82811 (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 1.5e-236
Identity = 407/530 (76.79%), Postives = 455/530 (85.85%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGD  G PGSSMHGVTGREQSFAFSV SPIV TD TAKF LPVD+EHKA VFK++S A P
Sbjct: 1   MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPLVPI+R+NLNLTK DIG+AGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFL+MLSAPTV  MSFVS+AAG+I V FMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF PG LHI MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVL LGQDLPDGN  +L+K G VA D F  ILWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY FDR  LKLHTAG+IAA FGM N  ARP GGYASD AA+YFGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GG+FC+WLG+AN   TA+VAM+LFS+GAQAACGATF I+PF SRR+LGIISGLTGAGGN
Sbjct: 361 AGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQL+FFST+ ++T     +MG+MIV CT+PV+LVHFPQWGSMFLPP+    K TE
Sbjct: 421 FGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
           A YYGSEW E EK+K +HQ S++ AEN+KSE GRRV SA TPP  TP++V
Sbjct: 481 AHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

BLAST of CcUC03G045000.1 vs. ExPASy Swiss-Prot
Match: Q9FJH8 (High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 PE=2 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 3.1e-234
Identity = 400/530 (75.47%), Postives = 455/530 (85.85%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           M D  G PGSSMHGVTGREQS+AFSV SP VP+D++AKF+LPVD+EHKAKVFK+ S   P
Sbjct: 1   MADGFGEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHL+WISFFTCF+STFAAAPLVPI+RDNLNLT+ D+G+AGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFL+MLSAPTV  MSFV  A GYI V FMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
            QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWR+AFF PG +HI MGI
Sbjct: 181 GQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGN ++L+KKG V  D FS +LWYA+TNYRTW+FVLLY +S+GVEL+ DN
Sbjct: 241 LVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY FDR  LKLHTAGIIAA+FGM N  ARP GG+ASD+AAR FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GG FC+WLG+A   PTA+V MILFS+GAQAACGATF IIPF SRRSLGIISGLTGAGGN
Sbjct: 361 LGGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFST+ +ST     +MG+MI+ CT+PV+LVHFPQWGSMFLP T+   KSTE
Sbjct: 421 FGSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            +YY  EWTE EK+KG+H+ S+K A NS+SERGRRVASAP+PP   P HV
Sbjct: 481 EYYYMKEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

BLAST of CcUC03G045000.1 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 6.4e-224
Identity = 386/529 (72.97%), Postives = 442/529 (83.55%), Query Frame = 0

Query: 6   GSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPHMRTF 65
           G+PGSS+HGVTGRE +FAFS           +KF LPVDSEHKAK  ++ S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 66  HLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGAVCDPL 125
           HLSWISFF+CFVSTFAAAPLVPI+RDNLNLTK DIG+AGV SVSGSIFSRL MGA+CD L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 126 GPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFNSQIIG 185
           GPRYGCAFLIML+APTV CMS + +AAGYIAV F+IGFSLATFVSCQYWMSTMFNS+IIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 186 LVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGILVLTL 245
           LVNG AAGWGNMGGGATQLIMPL+YDVI++ GATPFTAWR+A+F PG+LH+ MG+LVLTL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 246 GQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDNVIAEY 305
           GQDLPDGNL SLQKKG+V  D+FS +LWYAVTNYRTW+FVLLY +S+GVEL+ DNVIAEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 306 CFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVF 365
            +DR DL L  AGIIAA+FGM N+VARP GG  SD+ ARYFGMR RLW +WILQT GG F
Sbjct: 307 FYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAF 366

Query: 366 CMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGNFGRGL 425
           C+ LG+A+  PT++V M+LFS  AQAACGA FG+IPF SRRSLGIISG+TGAGGNFG GL
Sbjct: 367 CLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGL 426

Query: 426 TQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTEAFYYG 485
           TQL+FF++++YST T   +MGIMI+ CT+PV LVHFPQWGSMFLPP    E   E  YYG
Sbjct: 427 TQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYG 486

Query: 486 SEWTEDEKKKGLHQQSIKCAENSKSERGRR-----VASAPTPPNITPSH 530
           SEW+E EK KGLH  S+K AENS+SERGRR      A+A TPPN +P H
Sbjct: 487 SEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

BLAST of CcUC03G045000.1 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 6.4e-224
Identity = 386/529 (72.97%), Postives = 442/529 (83.55%), Query Frame = 0

Query: 6   GSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPHMRTF 65
           G+PGSS+HGVTGRE +FAFS           +KF LPVDSEHKAK  ++ S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 66  HLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGAVCDPL 125
           HLSWISFF+CFVSTFAAAPLVPI+RDNLNLTK DIG+AGV SVSGSIFSRL MGA+CD L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 126 GPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFNSQIIG 185
           GPRYGCAFLIML+APTV CMS + +AAGYIAV F+IGFSLATFVSCQYWMSTMFNS+IIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 186 LVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGILVLTL 245
           LVNG AAGWGNMGGGATQLIMPL+YDVI++ GATPFTAWR+A+F PG+LH+ MG+LVLTL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 246 GQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDNVIAEY 305
           GQDLPDGNL SLQKKG+V  D+FS +LWYAVTNYRTW+FVLLY +S+GVEL+ DNVIAEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 306 CFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVF 365
            +DR DL L  AGIIAA+FGM N+VARP GG  SD+ ARYFGMR RLW +WILQT GG F
Sbjct: 307 FYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAF 366

Query: 366 CMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGNFGRGL 425
           C+ LG+A+  PT++V M+LFS  AQAACGA FG+IPF SRRSLGIISG+TGAGGNFG GL
Sbjct: 367 CLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGL 426

Query: 426 TQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTEAFYYG 485
           TQL+FF++++YST T   +MGIMI+ CT+PV LVHFPQWGSMFLPP    E   E  YYG
Sbjct: 427 TQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYG 486

Query: 486 SEWTEDEKKKGLHQQSIKCAENSKSERGRR-----VASAPTPPNITPSH 530
           SEW+E EK KGLH  S+K AENS+SERGRR      A+A TPPN +P H
Sbjct: 487 SEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

BLAST of CcUC03G045000.1 vs. ExPASy Swiss-Prot
Match: Q9LMZ9 (High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 6.6e-213
Identity = 371/516 (71.90%), Postives = 434/516 (84.11%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MG  +  PGSSMHGVTGREQS+AFSV     PT+T  K+ LPVD+E KA VFK++S A P
Sbjct: 1   MGSTD-EPGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPL+PI+R+NLNLTK DIG+AGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFL+MLSAPTV  MSFVS+AAG+I V FMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF PG LHI MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLP GN  +++K G VA D F  ILWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY FDR  LKLHTAGIIAA FGM N  ARP GG+ASD+AA+ FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GG+FC+WLG+AN   TA+V+M+LFS+GAQAACGATF I+PF SRR+LGIISGLTGAGGN
Sbjct: 361 SGGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFST++++T     +MG+MIV CT+PV+L+HFPQWGSMF PP+     +TE
Sbjct: 421 FGSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRV 517
            +Y G E++++E++ G+H +S   A+ +K+E G  V
Sbjct: 481 HYYVG-EYSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

BLAST of CcUC03G045000.1 vs. ExPASy TrEMBL
Match: A0A3Q9CZ42 (High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 2.9e-280
Identity = 486/530 (91.70%), Postives = 502/530 (94.72%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPI+RDNLNLTKVDIG+AGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFLIMLSAPTV CMSFVSNAAGYIAV FMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFF PG LH+ MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGNL SLQKKGNVA D FSN+LWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY +DR DLKLHTAGIIAATFGM NLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA K P AI AMILFSIGAQAACGATFGIIPF SRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFSTTKYST T  F MGIMIVCCT+PV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            FYYGSEWTEDEKKKGLHQQSIK AENS+SERGRRVASAPTPPN TP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CcUC03G045000.1 vs. ExPASy TrEMBL
Match: Q6PRP8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3)

HSP 1 Score: 971.8 bits (2511), Expect = 1.1e-279
Identity = 486/530 (91.70%), Postives = 501/530 (94.53%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPI+RDNLNLTKVDIG+AGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFLIMLSAPTV CMSFVSNAAGYIAV FMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFF PG LH+ MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGNL SLQKKGNVA D FSN+LWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY +DR DLKLHTAGIIAATFGM NLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA K P AI AMILFSIGAQAACGATFGIIPF SRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFSTTKYST T  F MGIMIVCCT+PV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            FYYGSEWTEDEKKKGLHQQSIK AENS+SERGRRVASAPTPPN TPS V
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

BLAST of CcUC03G045000.1 vs. ExPASy TrEMBL
Match: S4VM42 (High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.5e-279
Identity = 484/530 (91.32%), Postives = 502/530 (94.72%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGDVEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPI+RDNLNLTKVDIG+AGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFLIMLSAPTV CMSFVS+AAGYIAV FMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFF PG LH+ MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGNL SLQKKGNVA D FSN+LWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY +DR DLKLHTAGIIAATFGM NLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA K P AI AMILFSIGAQAACGATFGIIPF SRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFSTTKYST T  F MGIMIVCCT+PV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            FYYGSEWTEDEKKKGLHQQSIK AENS+SERGRRVASAPTPPN TP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of CcUC03G045000.1 vs. ExPASy TrEMBL
Match: A0A6J1HIH1 (high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC111464708 PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 3.1e-274
Identity = 476/530 (89.81%), Postives = 498/530 (93.96%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           M  VEGSPGSSMHGVTGREQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVFK++S ANP
Sbjct: 1   MSGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPI+RDNLNLTKVDIG+AGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFLIMLSAPTV CMSFVS+AAGYIAV FMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGGATQLIMPLLYD+I+RAGATPFTAWRIAFF PG LH+ MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGNL+SLQKKG+VA D FS +LWYA+TNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY +DR DLKLHTAGIIAATFGM NLVARPFGGYASD AARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA+K PTAI AMILFSIGAQAACGATFGIIPF SRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFSTTKYST    F MGIMIVCCT+PV+LVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            FYYG+EWTEDEKKKGLHQQSIK AENS+SERGRRV SAPTPPN TPSHV
Sbjct: 481 EFYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of CcUC03G045000.1 vs. ExPASy TrEMBL
Match: A0A6J1DHB7 (high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC111020453 PE=4 SV=1)

HSP 1 Score: 953.4 bits (2463), Expect = 4.1e-274
Identity = 471/529 (89.04%), Postives = 499/529 (94.33%), Query Frame = 0

Query: 2   GDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPH 61
           G+VEGSPGSSMHGVTGREQ+FAFSVASPIVPTDT+AKFALPVDSEHKAKVFK++SLANPH
Sbjct: 6   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 65

Query: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGAV 121
           MRTFHLSWISFFTCFVSTFAAAPLVPI+RDNLNLTK DIG+AGVTSVSGSIFSRLVMGAV
Sbjct: 66  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 125

Query: 122 CDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFNS 181
           CD LGPRYGCAFLIMLSAPTV CMSFVS+ AGYIAV FMIGFSLATFVSCQYWMSTMFNS
Sbjct: 126 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 185

Query: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGIL 241
           QIIGLVNGTAAGWGNMGGGATQL+MPLLY++I++ GATPFTAWRIAFF PGSLH+ MGIL
Sbjct: 186 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 245

Query: 242 VLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDNV 301
           V+TLGQDLPDGNL+SLQKKG VA D FSN+LWYAVTNYRTW+FVLLY +S+GVELS DNV
Sbjct: 246 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 305

Query: 302 IAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 361
           +AEY +DR DLKLHTAGIIAATFGM NLVARPFGGYASD AARYFGMRGRLWTLWILQTC
Sbjct: 306 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 365

Query: 362 GGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGNF 421
           GGVFCMWLGKA + P AIVAMILFSIGAQAACGATFGIIPF SRRSLGIISGLTGAGGNF
Sbjct: 366 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 425

Query: 422 GRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTEA 481
           G GLTQLVFFSTTKYST    F MGIMIVCCT+PV+LVHFPQWGSMFLPPTKHVEKSTE 
Sbjct: 426 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 485

Query: 482 FYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
           FYYGSEWTEDEKKKGLHQQSIK AENS+SERGR++ASAPTPPN TPSHV
Sbjct: 486 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 534

BLAST of CcUC03G045000.1 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 820.1 bits (2117), Expect = 1.0e-237
Identity = 407/530 (76.79%), Postives = 455/530 (85.85%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MGD  G PGSSMHGVTGREQSFAFSV SPIV TD TAKF LPVD+EHKA VFK++S A P
Sbjct: 1   MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPLVPI+R+NLNLTK DIG+AGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFL+MLSAPTV  MSFVS+AAG+I V FMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF PG LHI MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVL LGQDLPDGN  +L+K G VA D F  ILWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY FDR  LKLHTAG+IAA FGM N  ARP GGYASD AA+YFGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GG+FC+WLG+AN   TA+VAM+LFS+GAQAACGATF I+PF SRR+LGIISGLTGAGGN
Sbjct: 361 AGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQL+FFST+ ++T     +MG+MIV CT+PV+LVHFPQWGSMFLPP+    K TE
Sbjct: 421 FGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
           A YYGSEW E EK+K +HQ S++ AEN+KSE GRRV SA TPP  TP++V
Sbjct: 481 AHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

BLAST of CcUC03G045000.1 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 812.4 bits (2097), Expect = 2.2e-235
Identity = 400/530 (75.47%), Postives = 455/530 (85.85%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           M D  G PGSSMHGVTGREQS+AFSV SP VP+D++AKF+LPVD+EHKAKVFK+ S   P
Sbjct: 1   MADGFGEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHL+WISFFTCF+STFAAAPLVPI+RDNLNLT+ D+G+AGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFL+MLSAPTV  MSFV  A GYI V FMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
            QIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWR+AFF PG +HI MGI
Sbjct: 181 GQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLPDGN ++L+KKG V  D FS +LWYA+TNYRTW+FVLLY +S+GVEL+ DN
Sbjct: 241 LVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY FDR  LKLHTAGIIAA+FGM N  ARP GG+ASD+AAR FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GG FC+WLG+A   PTA+V MILFS+GAQAACGATF IIPF SRRSLGIISGLTGAGGN
Sbjct: 361 LGGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFST+ +ST     +MG+MI+ CT+PV+LVHFPQWGSMFLP T+   KSTE
Sbjct: 421 FGSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASAPTPPNITPSHV 531
            +YY  EWTE EK+KG+H+ S+K A NS+SERGRRVASAP+PP   P HV
Sbjct: 481 EYYYMKEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

BLAST of CcUC03G045000.1 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 741.5 bits (1913), Expect = 4.7e-214
Identity = 371/516 (71.90%), Postives = 434/516 (84.11%), Query Frame = 0

Query: 1   MGDVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANP 60
           MG  +  PGSSMHGVTGREQS+AFSV     PT+T  K+ LPVD+E KA VFK++S A P
Sbjct: 1   MGSTD-EPGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPL+PI+R+NLNLTK DIG+AGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFN 180
           VCD LGPRYGCAFL+MLSAPTV  MSFVS+AAG+I V FMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGI 240
           SQIIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF PG LHI MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDN 300
           LVLTLGQDLP GN  +++K G VA D F  ILWYAVTNYRTW+FVLLY +S+GVELS DN
Sbjct: 241 LVLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEY FDR  LKLHTAGIIAA FGM N  ARP GG+ASD+AA+ FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGN 420
            GG+FC+WLG+AN   TA+V+M+LFS+GAQAACGATF I+PF SRR+LGIISGLTGAGGN
Sbjct: 361 SGGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTE 480
           FG GLTQLVFFST++++T     +MG+MIV CT+PV+L+HFPQWGSMF PP+     +TE
Sbjct: 421 FGSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATE 480

Query: 481 AFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRV 517
            +Y G E++++E++ G+H +S   A+ +K+E G  V
Sbjct: 481 HYYVG-EYSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

BLAST of CcUC03G045000.1 vs. TAIR 10
Match: AT5G60780.1 (nitrate transporter 2.3 )

HSP 1 Score: 735.7 bits (1898), Expect = 2.6e-212
Identity = 364/531 (68.55%), Postives = 431/531 (81.17%), Query Frame = 0

Query: 5   EGSPGSSMHGVTGREQSFAFSV---ASPIVPTDTTAKFALPVDSEHKAKVFKVWSLANPH 64
           EGS G+S+HGVT REQ F+FSV   +  +   D TAKFALPVDSEH+AKVF   S A PH
Sbjct: 9   EGSIGTSLHGVTAREQVFSFSVDASSQTVQSDDPTAKFALPVDSEHRAKVFNPLSFAKPH 68

Query: 65  MRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVMGAV 124
           MR FHL W+SFFTCF+STFAAAPLVPI+RDNL+LTK DIG+AGV SVSG+IFSRL MGAV
Sbjct: 69  MRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAMGAV 128

Query: 125 CDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTMFNS 184
           CD LG RYG AF +ML+APTV  MSFV   +GY+ V FMIGF LATFVSCQYW S MFN 
Sbjct: 129 CDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVMFNG 188

Query: 185 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPMGIL 244
           +IIGLVNG A GWG+MGGG TQL+MP+++ VI+ AGATPF AWRIAFF PG L + MGIL
Sbjct: 189 KIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVMGIL 248

Query: 245 VLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSHDNV 304
           VL+LGQDLPDGNL++LQK G V+ D FS + W+AV NYRTW+  +LY  S+G+EL+ +NV
Sbjct: 249 VLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTINNV 308

Query: 305 IAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 364
           I+ Y +DR +LKL TAGI+AA+FGM N +ARPFGGYASDVAAR FGMRGRLWTLWI QT 
Sbjct: 309 ISGYFYDRFNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIFQTV 368

Query: 365 GGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAGGNF 424
           G +FC+WLG+A+  P AI+AM+LFSIG QAACGA FG+ PF SRRSLG+ISGLTGAGGNF
Sbjct: 369 GALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAGGNF 428

Query: 425 GRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKSTEA 484
           G GLTQL+FFS+ ++ST      MG+M V CT+PV+ +HFPQWGSMFL P+   E+S E 
Sbjct: 429 GSGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERSQEE 488

Query: 485 FYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRRVASA--PTPPNITPSHV 531
           +YYGSEWTE+EK++GLH+ SIK AENS+SERGR+VA A  PTP N TPSHV
Sbjct: 489 YYYGSEWTENEKQQGLHEGSIKFAENSRSERGRKVALANIPTPENGTPSHV 539

BLAST of CcUC03G045000.1 vs. TAIR 10
Match: AT3G45060.1 (high affinity nitrate transporter 2.6 )

HSP 1 Score: 723.8 bits (1867), Expect = 1.0e-208
Identity = 359/534 (67.23%), Postives = 428/534 (80.15%), Query Frame = 0

Query: 5   EGSPGSSMHGVTGREQSFAFSV------ASPIVPTDTTAKFALPVDSEHKAKVFKVWSLA 64
           +GS G+S+HGVT REQ F+FSV      +  +   D TAKFALPVDSEH+AKVFK  S A
Sbjct: 9   DGSIGTSLHGVTAREQVFSFSVQEDVPSSQAVRTNDPTAKFALPVDSEHRAKVFKPLSFA 68

Query: 65  NPHMRTFHLSWISFFTCFVSTFAAAPLVPIMRDNLNLTKVDIGHAGVTSVSGSIFSRLVM 124
            PHMR FHL WISFFTCF+STFAAAPLVP++RDNL+LTK DIG+AGV SVSG+IFSRL M
Sbjct: 69  KPHMRAFHLGWISFFTCFISTFAAAPLVPVIRDNLDLTKTDIGNAGVASVSGAIFSRLAM 128

Query: 125 GAVCDPLGPRYGCAFLIMLSAPTVSCMSFVSNAAGYIAVSFMIGFSLATFVSCQYWMSTM 184
           GAVCD LG RYG AF +ML+AP V  MSFV++A  Y+AV FMIGF LATFVSCQYW S M
Sbjct: 129 GAVCDLLGARYGTAFSLMLTAPAVFSMSFVADAGSYLAVRFMIGFCLATFVSCQYWTSVM 188

Query: 185 FNSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFTPGSLHIPM 244
           F  +IIGLVNG A GWG+MGGG TQL+MP+++ VI+  GATPFTAWR AFF PG L I M
Sbjct: 189 FTGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLTGATPFTAWRFAFFIPGILQIVM 248

Query: 245 GILVLTLGQDLPDGNLTSLQKKGNVANDTFSNILWYAVTNYRTWLFVLLYRFSIGVELSH 304
           GILVLTLGQDLPDGNL++LQK G V+ D FS + W+AV NYRTW+  +LY FS+GVEL+ 
Sbjct: 249 GILVLTLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFMLYGFSMGVELTI 308

Query: 305 DNVIAEYCFDRLDLKLHTAGIIAATFGMVNLVARPFGGYASDVAARYFGMRGRLWTLWIL 364
           +NVI+ Y +DR +L LHTAGIIAA+FGM N  ARPFGGYASDVAAR FGMRGRLW LWIL
Sbjct: 309 NNVISGYFYDRFNLTLHTAGIIAASFGMANFFARPFGGYASDVAARLFGMRGRLWILWIL 368

Query: 365 QTCGGVFCMWLGKANKRPTAIVAMILFSIGAQAACGATFGIIPFSSRRSLGIISGLTGAG 424
           QT G +FC+WLG+A+  P AI+AM+LFS+G QAACGA FG+ PF SRRSLG+ISGLTGAG
Sbjct: 369 QTVGALFCIWLGRASSLPIAILAMMLFSMGTQAACGALFGVAPFVSRRSLGLISGLTGAG 428

Query: 425 GNFGRGLTQLVFFSTTKYSTVTCFFFMGIMIVCCTVPVSLVHFPQWGSMFLPPTKHVEKS 484
           GNFG G+TQL+FFS++++ST      MG+M V C++PV+ +HFPQWGSMFL P++  EKS
Sbjct: 429 GNFGSGVTQLLFFSSSRFSTAEGLSLMGVMAVVCSLPVAFIHFPQWGSMFLRPSQDGEKS 488

Query: 485 TEAFYYGSEWTEDEKKKGLHQQSIKCAENSKSERGRR--VASAPTPPNITPSHV 531
            E  YYG+EWTE+EK  GLH+ SIK AENS+SERGR+  +A  PTP   +P+HV
Sbjct: 489 KEEHYYGAEWTEEEKSLGLHEGSIKFAENSRSERGRKAMLADIPTPETGSPAHV 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895485.12.2e-28292.64high affinity nitrate transporter 2.4-like [Benincasa hispida] >XP_038895611.1 h... [more]
XP_031736393.16.1e-28091.70high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP5371... [more]
NP_001274401.12.3e-27991.70high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-af... [more]
AGO64298.13.0e-27991.32high-affinity nitrate transporter NRT2.2 [Cucumis sativus][more]
NP_001269063.24.3e-27891.13high affinity nitrate transporter 2.4-like [Cucumis sativus][more]
Match NameE-valueIdentityDescription
O828111.5e-23676.79High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 ... [more]
Q9FJH83.1e-23475.47High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 ... [more]
P0DKG96.4e-22472.97High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
P0DKH06.4e-22472.97High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9LMZ96.6e-21371.90High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 ... [more]
Match NameE-valueIdentityDescription
A0A3Q9CZ422.9e-28091.70High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 ... [more]
Q6PRP81.1e-27991.70High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3[more]
S4VM421.5e-27991.32High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE... [more]
A0A6J1HIH13.1e-27489.81high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1DHB74.1e-27489.04high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT1G08090.11.0e-23776.79nitrate transporter 2:1 [more]
AT5G60770.12.2e-23575.47nitrate transporter 2.4 [more]
AT1G08100.14.7e-21471.90nitrate transporter 2.2 [more]
AT5G60780.12.6e-21268.55nitrate transporter 2.3 [more]
AT3G45060.11.0e-20867.23high affinity nitrate transporter 2.6 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 58..254
e-value: 7.4E-18
score: 66.7
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 268..463
e-value: 1.3E-24
score: 88.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 58..460
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 69..425
e-value: 1.1E-15
score: 57.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 493..513
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 493..530
NoneNo IPR availablePANTHERPTHR23515:SF16HIGH-AFFINITY NITRATE TRANSPORTER 2.1-LIKEcoord: 1..527
IPR044772Nitrate transporterPANTHERPTHR23515HIGH-AFFINITY NITRATE TRANSPORTER 2.3coord: 1..527
IPR044772Nitrate transporterCDDcd17341MFS_NRT2_likecoord: 65..458
e-value: 1.67702E-124
score: 368.117

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CcUC03G045000CcUC03G045000gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC03G045000.1-exonCcUC03G045000.1-exon-CicolChr03:2969658..2970467exon
CcUC03G045000.1-exonCcUC03G045000.1-exon-CicolChr03:2970582..2970697exon
CcUC03G045000.1-exonCcUC03G045000.1-exon-CicolChr03:2971003..2971669exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC03G045000.1-cdsCcUC03G045000.1-cds-CicolChr03:2969658..2970467CDS
CcUC03G045000.1-cdsCcUC03G045000.1-cds-CicolChr03:2970582..2970697CDS
CcUC03G045000.1-cdsCcUC03G045000.1-cds-CicolChr03:2971003..2971669CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CcUC03G045000.1CcUC03G045000.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0071249 cellular response to nitrate
biological_process GO:0042128 nitrate assimilation
biological_process GO:0015706 nitrate transport
biological_process GO:0031204 posttranslational protein targeting to membrane, translocation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0071261 Ssh1 translocon complex
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity
molecular_function GO:0008320 protein transmembrane transporter activity