CcUC03G044850.1 (mRNA) Watermelon (PI 537277) v1

Overview
NameCcUC03G044850.1
TypemRNA
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionProtein NUCLEAR FUSION DEFECTIVE 4
LocationCicolChr03: 2817788 .. 2819846 (-)
Sequence length1776
RNA-Seq ExpressionCcUC03G044850.1
SyntenyCcUC03G044850.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGTAGCAGCAGGGTCGAGCTCCAATGGCGGAATTGGGAGCGGAGAATTGGCCAATTTTATCCGGCAAGTGGTGGTGGGCCGGTGGTTTTCTCTTTTTGCTTCGTTCCTTGTAATGACCGGCGCCGGCGGAGTTTATTTGTTCGCTTATTACTCTAGAGACATCAAAAAGACGCTCCAATGTGATCAAACGACACTAAACAAAATTGGGTTTTACAAAGATTTGGGATCCAACGTCGGCGTTATTGCCGGACTTCTGGCGGAGGTGGCGCCAACTTGGCTCGTCCTTCTCATCGGCGCCGCCTTCAATTTTCTTGGCTACTTTAAGATATGGCAAGCCGTCACCGGAAAAATTGTTCAGCCCACCGTCGCCTTCTTTTGCTTTTACATTATGGTTGGAGCTAATTCTCAGAATTTTGCCAATACAGGTACTGCAAATTAATTAATAACTCTAAATTTTATTTTATTTTTAGTATAATTATTTTAAATGAAAAAATGACTAGAAATATCTCCGTTAATAGCAAAATACCATTTATTACTCTAATAAATCCAATAGATTACATCATCTATTTCTGTTTATTTCTGTTTATCACTAATAGATATTCTATTAACACTATAACCGGTTAATGATAAACTTCAAAAAAAAAATTAGAGTTAAATTTTGCTTTATGTTTATCAACTAATTTAGTGTTTTTTCTTGAAACACAACAGGAGTACTTGTCACGTGCGTGAAGAACTTTCCAGAAAGAAGAGGAGTGATGTTGGGCCTTCTAAAGGGCTTTGTGGGCCTAAGCGGAGCTATCATGACCCAACTTTACATTGCTATTTACGGCCATGATACCAAATCTCTTGTTCTTCTAATCGCATGGTTTCCTTCTATAGTCTCTTTGATCTTCGTCTACACAATCAGAGAAATCAAAGCCGTAAAACACCCAAATGAATTTAGAGTTTTCGTTCAATTCCTTTGCGTCACAGTCCTTTTAGCCATTTTCCTCACCGTCTTAGTCTTCGTTCAAAATCGAGTCCGATTCCATCAATCAGCTCACATCGCCATTGTCTCTGCGATTCTTGCTCTGCTTATTGTTCCATTGTTAATCGCCATTAGAGAAGAACTCCTTCTCTGGAATCTCAATAAACGAACTATTCCTACAAACCCTTTCACCAGAATTAGAATCGAAAACAACCCCCAGACAACAACCCCAATTCCTTCTTCAAATCAAACTCAATTCAGTTCTTGTTTCGCTAACATTTTCAACAAACCCGAAAGAGGCGAAGATTACACAGTTCTTCAAGCGATTTTCAGTATCGATATGCTTATTTTATGTTCCACTATGTTAATCGGCGTCGGTGCAAGCTTAACCGCCATTGACAATCTAGGGCAAATCGGTGAATCCCAAGGCTACCCATCTGAAACAATCAACTCATTCATCTCTTTAGTAAGTATTTTCAACTTCAGTGGTAGAATCTTTTCTGGGTTCGTCTCGGAAATCTTGCTTGAGAAATTCAAATTCCCTAGGCCATTGATGCTCACACTCATCCTCTTGATTTCCTGTATTGGGCATCTTTTGGTAGCCTTTCCCTTTGAGGATTCGCTCTACGTTGCTTCAATCATAATTGGATTTTCACTGGGGTCTCAAGTTCCATTGCATTTCGCGATGATTTCAGAGATTTTTGGGCTGAAACATTACTCGACTTTGTTTAATTTTGGGCAATTGTCTTGTCCAATTGGGTCTTATATTCTGAATGTAATGGTGACTGGGAAGATGTATGATAAGGTAGCGCAATTGGGAAGTAATCCAAACAAGTTTCATTGTGCAGGAAGTCATTGTTATGAACAATCGTTTTTGATTTTGGGTGGATTAACGTTTATTGTAGCGATGATATCGTTGATTTTGGTGATGAGGACGAGGGATTTTTATAGAGGGGATATATACAAGAAATTCAGGGAAGATATGGAATCTTTGAAGACGGAAGTCGAGTTTTACAAAGTGGATGAGAAGAGGACTAGGATTGGGAATTTACTTGTTGATAAATATACCATAAATTTCCAGAGGTGA

mRNA sequence

ATGATTGTAGCAGCAGGGTCGAGCTCCAATGGCGGAATTGGGAGCGGAGAATTGGCCAATTTTATCCGGCAAGTGGTGGTGGGCCGGTGGTTTTCTCTTTTTGCTTCGTTCCTTGTAATGACCGGCGCCGGCGGAGTTTATTTGTTCGCTTATTACTCTAGAGACATCAAAAAGACGCTCCAATGTGATCAAACGACACTAAACAAAATTGGGTTTTACAAAGATTTGGGATCCAACGTCGGCGTTATTGCCGGACTTCTGGCGGAGGTGGCGCCAACTTGGCTCGTCCTTCTCATCGGCGCCGCCTTCAATTTTCTTGGCTACTTTAAGATATGGCAAGCCGTCACCGGAAAAATTGTTCAGCCCACCGTCGCCTTCTTTTGCTTTTACATTATGGTTGGAGCTAATTCTCAGAATTTTGCCAATACAGGAGTACTTGTCACGTGCGTGAAGAACTTTCCAGAAAGAAGAGGAGTGATGTTGGGCCTTCTAAAGGGCTTTGTGGGCCTAAGCGGAGCTATCATGACCCAACTTTACATTGCTATTTACGGCCATGATACCAAATCTCTTGTTCTTCTAATCGCATGGTTTCCTTCTATAGTCTCTTTGATCTTCGTCTACACAATCAGAGAAATCAAAGCCGTAAAACACCCAAATGAATTTAGAGTTTTCGTTCAATTCCTTTGCGTCACAGTCCTTTTAGCCATTTTCCTCACCGTCTTAGTCTTCGTTCAAAATCGAGTCCGATTCCATCAATCAGCTCACATCGCCATTGTCTCTGCGATTCTTGCTCTGCTTATTGTTCCATTGTTAATCGCCATTAGAGAAGAACTCCTTCTCTGGAATCTCAATAAACGAACTATTCCTACAAACCCTTTCACCAGAATTAGAATCGAAAACAACCCCCAGACAACAACCCCAATTCCTTCTTCAAATCAAACTCAATTCAGTTCTTGTTTCGCTAACATTTTCAACAAACCCGAAAGAGGCGAAGATTACACAGTTCTTCAAGCGATTTTCAGTATCGATATGCTTATTTTATGTTCCACTATGTTAATCGGCGTCGGTGCAAGCTTAACCGCCATTGACAATCTAGGGCAAATCGGTGAATCCCAAGGCTACCCATCTGAAACAATCAACTCATTCATCTCTTTAGTAAGTATTTTCAACTTCAGTGGTAGAATCTTTTCTGGGTTCGTCTCGGAAATCTTGCTTGAGAAATTCAAATTCCCTAGGCCATTGATGCTCACACTCATCCTCTTGATTTCCTGTATTGGGCATCTTTTGGTAGCCTTTCCCTTTGAGGATTCGCTCTACGTTGCTTCAATCATAATTGGATTTTCACTGGGGTCTCAAGTTCCATTGCATTTCGCGATGATTTCAGAGATTTTTGGGCTGAAACATTACTCGACTTTGTTTAATTTTGGGCAATTGTCTTGTCCAATTGGGTCTTATATTCTGAATGTAATGGTGACTGGGAAGATGTATGATAAGGTAGCGCAATTGGGAAGTAATCCAAACAAGTTTCATTGTGCAGGAAGTCATTGTTATGAACAATCGTTTTTGATTTTGGGTGGATTAACGTTTATTGTAGCGATGATATCGTTGATTTTGGTGATGAGGACGAGGGATTTTTATAGAGGGGATATATACAAGAAATTCAGGGAAGATATGGAATCTTTGAAGACGGAAGTCGAGTTTTACAAAGTGGATGAGAAGAGGACTAGGATTGGGAATTTACTTGTTGATAAATATACCATAAATTTCCAGAGGTGA

Coding sequence (CDS)

ATGATTGTAGCAGCAGGGTCGAGCTCCAATGGCGGAATTGGGAGCGGAGAATTGGCCAATTTTATCCGGCAAGTGGTGGTGGGCCGGTGGTTTTCTCTTTTTGCTTCGTTCCTTGTAATGACCGGCGCCGGCGGAGTTTATTTGTTCGCTTATTACTCTAGAGACATCAAAAAGACGCTCCAATGTGATCAAACGACACTAAACAAAATTGGGTTTTACAAAGATTTGGGATCCAACGTCGGCGTTATTGCCGGACTTCTGGCGGAGGTGGCGCCAACTTGGCTCGTCCTTCTCATCGGCGCCGCCTTCAATTTTCTTGGCTACTTTAAGATATGGCAAGCCGTCACCGGAAAAATTGTTCAGCCCACCGTCGCCTTCTTTTGCTTTTACATTATGGTTGGAGCTAATTCTCAGAATTTTGCCAATACAGGAGTACTTGTCACGTGCGTGAAGAACTTTCCAGAAAGAAGAGGAGTGATGTTGGGCCTTCTAAAGGGCTTTGTGGGCCTAAGCGGAGCTATCATGACCCAACTTTACATTGCTATTTACGGCCATGATACCAAATCTCTTGTTCTTCTAATCGCATGGTTTCCTTCTATAGTCTCTTTGATCTTCGTCTACACAATCAGAGAAATCAAAGCCGTAAAACACCCAAATGAATTTAGAGTTTTCGTTCAATTCCTTTGCGTCACAGTCCTTTTAGCCATTTTCCTCACCGTCTTAGTCTTCGTTCAAAATCGAGTCCGATTCCATCAATCAGCTCACATCGCCATTGTCTCTGCGATTCTTGCTCTGCTTATTGTTCCATTGTTAATCGCCATTAGAGAAGAACTCCTTCTCTGGAATCTCAATAAACGAACTATTCCTACAAACCCTTTCACCAGAATTAGAATCGAAAACAACCCCCAGACAACAACCCCAATTCCTTCTTCAAATCAAACTCAATTCAGTTCTTGTTTCGCTAACATTTTCAACAAACCCGAAAGAGGCGAAGATTACACAGTTCTTCAAGCGATTTTCAGTATCGATATGCTTATTTTATGTTCCACTATGTTAATCGGCGTCGGTGCAAGCTTAACCGCCATTGACAATCTAGGGCAAATCGGTGAATCCCAAGGCTACCCATCTGAAACAATCAACTCATTCATCTCTTTAGTAAGTATTTTCAACTTCAGTGGTAGAATCTTTTCTGGGTTCGTCTCGGAAATCTTGCTTGAGAAATTCAAATTCCCTAGGCCATTGATGCTCACACTCATCCTCTTGATTTCCTGTATTGGGCATCTTTTGGTAGCCTTTCCCTTTGAGGATTCGCTCTACGTTGCTTCAATCATAATTGGATTTTCACTGGGGTCTCAAGTTCCATTGCATTTCGCGATGATTTCAGAGATTTTTGGGCTGAAACATTACTCGACTTTGTTTAATTTTGGGCAATTGTCTTGTCCAATTGGGTCTTATATTCTGAATGTAATGGTGACTGGGAAGATGTATGATAAGGTAGCGCAATTGGGAAGTAATCCAAACAAGTTTCATTGTGCAGGAAGTCATTGTTATGAACAATCGTTTTTGATTTTGGGTGGATTAACGTTTATTGTAGCGATGATATCGTTGATTTTGGTGATGAGGACGAGGGATTTTTATAGAGGGGATATATACAAGAAATTCAGGGAAGATATGGAATCTTTGAAGACGGAAGTCGAGTTTTACAAAGTGGATGAGAAGAGGACTAGGATTGGGAATTTACTTGTTGATAAATATACCATAAATTTCCAGAGGTGA

Protein sequence

MIVAAGSSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIVQPTVAFFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLVFVQNRVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNPQTTTPIPSSNQTQFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVGASLTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLMLTLILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMISLILVMRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQR
Homology
BLAST of CcUC03G044850.1 vs. NCBI nr
Match: XP_038895750.1 (uncharacterized protein LOC120083915 [Benincasa hispida])

HSP 1 Score: 1031.6 bits (2666), Expect = 2.7e-297
Identity = 529/601 (88.02%), Postives = 568/601 (94.51%), Query Frame = 0

Query: 1   MIVAAGSSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTL 60
           MIVAAG SSNGGIGSGELA+F+RQVVVGRWFSLFASFLVM+GAGGVY+FAYYS+DIK TL
Sbjct: 1   MIVAAGHSSNGGIGSGELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIV 120
           +CDQTTLNKIGFYKDLGSNVG++AGLLAEVAPTWLVLLIGAA NFLGYFKIWQAVTGKIV
Sbjct: 61  KCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKIV 120

Query: 121 QPTVAFFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180
            PTV FFCF+IMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI
Sbjct: 121 HPTVGFFCFFIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGHDTKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTV 240
           AIYGHDT+SLVLLIAWFPS++SLIFVYTIREIK VKHPNEFRVFVQFLC+T+LL++FLTV
Sbjct: 181 AIYGHDTRSLVLLIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLTV 240

Query: 241 LVFVQNRVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIEN 300
           L+F+Q RV+F QSAHIAIV+AI ALL VPLLIAIREEL+LWN NKRT+  NPFTRIRIE 
Sbjct: 241 LIFIQKRVQFDQSAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIE- 300

Query: 301 NPQTTT--------PIPSSNQTQF--SSCFANIFNKPERGEDYTVLQAIFSIDMLILCST 360
            PQTTT        PIPSSNQTQ   SSCFANIFNKPERGEDYTVLQAIFSIDMLI+CST
Sbjct: 301 TPQTTTTTTTTTNPPIPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICST 360

Query: 361 MLIGVGASLTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKF 420
           MLIGVGASLTAIDNLGQIGESQGYPS TIN FISLVSIFNF+GRIFSGFVSEILLEKFKF
Sbjct: 361 MLIGVGASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKF 420

Query: 421 PRPLMLTLILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYS 480
           PRPLMLT ILLISC+GHLLVAFPFEDSLY+ASIIIGFS+GSQVPLHFAMISEIFGLKHYS
Sbjct: 421 PRPLMLTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYS 480

Query: 481 TLFNFGQLSCPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFI 540
           TLFNFGQLSCPIGSYILNVMVTGK+YD+VAQLGS+PNKFHC G+HCYEQSFLIL GLTFI
Sbjct: 481 TLFNFGQLSCPIGSYILNVMVTGKLYDEVAQLGSSPNKFHCQGNHCYEQSFLILSGLTFI 540

Query: 541 VAMISLILVMRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQ 592
           VAMISLILV RTR+FY GDIYKKFREDME+LKTE+EFY++DEKRTRIGNLLVDK++INF+
Sbjct: 541 VAMISLILVKRTREFYGGDIYKKFREDMETLKTEMEFYRLDEKRTRIGNLLVDKHSINFK 600

BLAST of CcUC03G044850.1 vs. NCBI nr
Match: XP_008464809.1 (PREDICTED: uncharacterized protein LOC103502605 [Cucumis melo])

HSP 1 Score: 1006.5 bits (2601), Expect = 9.4e-290
Identity = 520/597 (87.10%), Postives = 561/597 (93.97%), Query Frame = 0

Query: 1   MIVAAGSSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTL 60
           MIVAAG  SNGGI SGELA+FI QVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIK TL
Sbjct: 1   MIVAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIV 120
           QCDQTTLN+IGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNF+GYFKIWQAVTGKIV
Sbjct: 61  QCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIV 120

Query: 121 QPTVAFFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180
           +PTVAFFCFYIM+GANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGHDTKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTV 240
           AIYGHDTKSL+LLIAWFPSIVSLIFVYTIREIK VKHPNEFRVFVQFLCVTVLLA+ LT 
Sbjct: 181 AIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTA 240

Query: 241 LVFVQNRVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIEN 300
           L+FVQ RV F QSAHIAIV+AILALL VPLLIAIREE+++WNLNKRT  +NPF RI IE+
Sbjct: 241 LIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIES 300

Query: 301 NPQTTTPIPSSN------QTQFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIG 360
           + Q   PI S +      QTQ +SCF+NIFNKPERGEDYTVLQAIFSIDMLI+CSTMLIG
Sbjct: 301 S-QPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIG 360

Query: 361 VGASLTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPL 420
           VGASLTAIDNLGQIGE+QGYPSETIN F+SL+SI NF+GRIFSGFVSEILLEKF+FPRPL
Sbjct: 361 VGASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPL 420

Query: 421 MLTLILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFN 480
           MLTLILLIS +GHLLVAFPF+DSLY+ASIIIGFS+GSQVPLHFAMISEIFGLKHYSTLFN
Sbjct: 421 MLTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFN 480

Query: 481 FGQLSCPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMI 540
           FGQLSCPIGSYILNVMVTGK+YD+VA++GSNPNK HC G+HCYEQSFLIL GLTFIVAMI
Sbjct: 481 FGQLSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMI 540

Query: 541 SLILVMRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQR 592
           SLILV RTR+FYRGDIYKKF+EDME+LKTEVEFY+VDEKRTRIGNLLVDK++INF++
Sbjct: 541 SLILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 596

BLAST of CcUC03G044850.1 vs. NCBI nr
Match: KAA0051898.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ99888.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.3e-288
Identity = 517/595 (86.89%), Postives = 559/595 (93.95%), Query Frame = 0

Query: 3   VAAGSSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQC 62
           +AAG  SNGGI SGELA+FI QVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIK TLQC
Sbjct: 28  LAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQC 87

Query: 63  DQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIVQP 122
           DQTTLN+IGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNF+GYFKIWQAVTGKIV+P
Sbjct: 88  DQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRP 147

Query: 123 TVAFFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAI 182
           TVAFFCFYIM+GANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAI
Sbjct: 148 TVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAI 207

Query: 183 YGHDTKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLV 242
           YGHDTKSL+LLIAWFPSIVSLIFVYTIREIK VKHPNEFRVFVQFLCVTVLLA+ LT L+
Sbjct: 208 YGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALI 267

Query: 243 FVQNRVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNP 302
           FVQ RV F QSAHIAIV+AILALL VPLLIAIREE+++WNLNKRT  +NPF RI IE++ 
Sbjct: 268 FVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESS- 327

Query: 303 QTTTPIPSSN------QTQFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVG 362
           Q   PI S +      QTQ +SCF+NIFNKPERGEDYTVLQAIFSIDMLI+CSTMLIGVG
Sbjct: 328 QPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVG 387

Query: 363 ASLTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLML 422
           ASLTAIDNLGQIGE+QGYPSETIN F+SL+SI NF+GRIFSGFVSEILLEKF+FPRPLML
Sbjct: 388 ASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLML 447

Query: 423 TLILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFG 482
           TLILLIS +GHLLVAFPF+DSLY+ASIIIGFS+GSQVPLHFAMISEIFGLKHYSTLFNFG
Sbjct: 448 TLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFG 507

Query: 483 QLSCPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMISL 542
           QLSCPIGSYILNVMVTGK+YD+VA++GSNPNK HC G+HCYEQSFLIL GLTFIVAMISL
Sbjct: 508 QLSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMISL 567

Query: 543 ILVMRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQR 592
           ILV RTR+FYRGDIYKKF+EDME+LKTEVEFY+VDEKRTRIGNLLVDK++INF++
Sbjct: 568 ILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 621

BLAST of CcUC03G044850.1 vs. NCBI nr
Match: XP_004147303.3 (uncharacterized protein LOC101202941 [Cucumis sativus] >KGN64782.2 hypothetical protein Csa_013229 [Cucumis sativus])

HSP 1 Score: 991.1 bits (2561), Expect = 4.1e-285
Identity = 501/601 (83.36%), Postives = 556/601 (92.51%), Query Frame = 0

Query: 1   MIVAAGSSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTL 60
           MIVAAG  SNGGIGSGEL +F+RQ+V GRWFSLFASFLVM GAGGVYLFAYYSRDIK TL
Sbjct: 1   MIVAAGQRSNGGIGSGELCDFVRQMVAGRWFSLFASFLVMAGAGGVYLFAYYSRDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIV 120
           QCDQTTLNKIGFYKDLGSNVGV AGLLAEV PTWL+LLIGAAFNF+GYFKIWQAVTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGVFAGLLAEVVPTWLLLLIGAAFNFMGYFKIWQAVTGKIV 120

Query: 121 QPTVAFFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180
           +PTVAFFCFYIM+GANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQ YI
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQFYI 180

Query: 181 AIYGHDTKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTV 240
           AIYGHDTKSLVLL+AWFPS++SL+FVYTIREIK+VKHPNEFRVF+QFLCVTVLL I LTV
Sbjct: 181 AIYGHDTKSLVLLLAWFPSLISLLFVYTIREIKSVKHPNEFRVFIQFLCVTVLLTILLTV 240

Query: 241 LVFVQNRVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIEN 300
           ++F+Q R+ F QSAHIAIV+AILALL VPLLIAIREE++LWNLNKRT  +NPFTRI+IE 
Sbjct: 241 IIFIQKRIHFDQSAHIAIVAAILALLFVPLLIAIREEVVLWNLNKRTRISNPFTRIKIET 300

Query: 301 NPQTTTPI----------PSSNQTQFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCST 360
           + QT +P+          P   QTQ +SCF+ IFNKPERGEDYTVLQAIFSIDMLI+C T
Sbjct: 301 S-QTNSPVDSPSTSQHPHPHPPQTQPTSCFSKIFNKPERGEDYTVLQAIFSIDMLIICFT 360

Query: 361 MLIGVGASLTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKF 420
           M+IGVGASLTAIDNLGQIGE+Q Y SETIN  +SL+SIFNF+GRIFSGFVSEILLEKF+F
Sbjct: 361 MMIGVGASLTAIDNLGQIGEAQAYSSETINLLVSLMSIFNFAGRIFSGFVSEILLEKFQF 420

Query: 421 PRPLMLTLILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYS 480
           PRPLMLTLILLISC+GHLLVAFPF+DSLYVASIIIGFS+GSQVPLHFAMISEIFGLKHYS
Sbjct: 421 PRPLMLTLILLISCLGHLLVAFPFDDSLYVASIIIGFSMGSQVPLHFAMISEIFGLKHYS 480

Query: 481 TLFNFGQLSCPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFI 540
           TLFNFGQLSCPIGSYILNV+VTGK+YD+VA++GSNPN  HC G+HCYE+SFLIL GLTF+
Sbjct: 481 TLFNFGQLSCPIGSYILNVLVTGKLYDEVARIGSNPNMLHCVGTHCYERSFLILAGLTFM 540

Query: 541 VAMISLILVMRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQ 592
           VAM+SLILV RTR+FYRGDIYKKFREDME+LKTEVEFY+VDEK+TRIGNLLVDK++I+F+
Sbjct: 541 VAMVSLILVKRTREFYRGDIYKKFREDMETLKTEVEFYRVDEKKTRIGNLLVDKHSIHFK 600

BLAST of CcUC03G044850.1 vs. NCBI nr
Match: XP_022998867.1 (uncharacterized protein LOC111493395 [Cucurbita maxima])

HSP 1 Score: 899.0 bits (2322), Expect = 2.1e-257
Identity = 457/593 (77.07%), Postives = 529/593 (89.21%), Query Frame = 0

Query: 7   SSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQCDQTT 66
           SS NGGI   EL+ FI+QVV GRWFSLFASFL+M+GAGGVYLFA YS+DIK TL+CDQTT
Sbjct: 8   SSINGGICRAELSCFIQQVVTGRWFSLFASFLIMSGAGGVYLFASYSKDIKSTLKCDQTT 67

Query: 67  LNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIVQPTVAF 126
           LNKIGFYKDLGSNVGVIAGLLAEVAPTW V+LIGAA NF+GYFKIWQAVTGKI++PTV F
Sbjct: 68  LNKIGFYKDLGSNVGVIAGLLAEVAPTWFVILIGAALNFVGYFKIWQAVTGKIIRPTVEF 127

Query: 127 FCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHD 186
           FCFYIMVGANSQNFANT VLV+CVKNFPERRGVMLGLLKGFVGLSGAIMTQ+Y A Y HD
Sbjct: 128 FCFYIMVGANSQNFANTVVLVSCVKNFPERRGVMLGLLKGFVGLSGAIMTQIYGAFYDHD 187

Query: 187 TKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLVFVQN 246
           TKSL+LL+AWFPS++S+IFV TIRE++AVKHPNEFRVFVQFLCVT++LA+FLT L+FVQ 
Sbjct: 188 TKSLILLLAWFPSLISIIFVKTIREMRAVKHPNEFRVFVQFLCVTIILALFLTALIFVQK 247

Query: 247 RVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNPQTTT 306
           +V+F+Q+AHI IV+AILALL+VPLLIAIREE+++WNLNKRT+  N FTRIRI+ NPQ+  
Sbjct: 248 KVQFNQTAHITIVAAILALLLVPLLIAIREEIIMWNLNKRTV-GNRFTRIRID-NPQSAP 307

Query: 307 PIPSSNQT--------QFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVGAS 366
              +S  +        Q +SCFANIFNKP+RGED+T+ QAIFS+DMLIL +TMLIGVGA+
Sbjct: 308 SCSASCSSSSVLELPKQSNSCFANIFNKPKRGEDFTIPQAIFSVDMLILLTTMLIGVGAN 367

Query: 367 LTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLMLTL 426
           LTAIDNLGQIGES  YP ETINSFISLVSIFNF+GRIFSGFVSEILLEKFKFPRP+MLTL
Sbjct: 368 LTAIDNLGQIGESLNYPPETINSFISLVSIFNFTGRIFSGFVSEILLEKFKFPRPMMLTL 427

Query: 427 ILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFGQL 486
           I L+SC+G+L+VAFPF +SL +ASIIIGFSLGSQVPLHFAMISE FGLKHYSTLFNFGQL
Sbjct: 428 IHLVSCVGYLIVAFPFNNSLCIASIIIGFSLGSQVPLHFAMISEFFGLKHYSTLFNFGQL 487

Query: 487 SCPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMISLIL 546
           SCPIGSYILNVMV GK+YD++A  G+N ++FHC GS C+EQSF IL GLTF+VAM+SL+L
Sbjct: 488 SCPIGSYILNVMVIGKLYDEMALTGNNASEFHCEGSKCFEQSFTILAGLTFVVAMVSLVL 547

Query: 547 VMRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQR 592
           V RT +FYRGDIY+KFREDM+SLKTE+EFY ++ KR +IGNL  DK+ INF++
Sbjct: 548 VRRTAEFYRGDIYQKFREDMDSLKTEMEFYPLESKRMKIGNLTFDKHAINFKK 598

BLAST of CcUC03G044850.1 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 6.2e-26
Identity = 132/547 (24.13%), Postives = 229/547 (41.86%), Query Frame = 0

Query: 29  RWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQCDQTTLNKIGFYKDLGSNVGVIAGLLA 88
           +W  L A+  +    G  + F+ YS  +K  L   Q  LN +    DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 89  EVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIVQPTVAFFCFYIMVGANSQNFANTGVLVT 148
              P  +VL   AA  F+GY   W  +T  I  P    F   ++ G +   F NT   + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGLSICWF-NTACFIL 162

Query: 149 CVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTKSLVLLIAWFPSIVSLIFVY- 208
           C+++FP  R + L L   F G+S A+ +  + AI    +   +LL +  P +VS   +Y 
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYP 222

Query: 209 --------TIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLVFVQNRVRFHQSAHIAIV 268
                   T  +  + +H +     +  L V     + L+       R+ F     I  V
Sbjct: 223 VLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNF-----IGAV 282

Query: 269 SAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNPQTTTPIPSSNQTQFSSC 328
             ++  L  PLL+  R+  L     +    ++ +  + I+      T + S    +    
Sbjct: 283 VLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGT 342

Query: 329 FANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVGASLTAIDNLGQIGESQGYPSETI 388
            A   N    G++++    I  ++  +       G    L   +NLGQI +S G  S T+
Sbjct: 343 -AKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTTL 402

Query: 389 NSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLMLTLILLISCIGHLLVAFPF--EDS 448
              +++ S F+F GR+ S    + + ++F+  R     + LL + I   L+A     + +
Sbjct: 403 ---VTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTA 462

Query: 449 LYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIGSYILNVMVTGKMYD 508
           L  A+ +IG S G       ++ S++FG        N    + PIGS +L   +   +Y+
Sbjct: 463 LQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIAASIYE 522

Query: 509 KVAQLGSNP---NKFHCAGSHCYEQSFLILGGLTFIVAMISLILVMRTRDFYRGDIYKKF 562
             A     P   +   C G  CY ++F+  G L+ +  + SL L +RT+      +Y + 
Sbjct: 523 ANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTK-----PVYHRL 572

BLAST of CcUC03G044850.1 vs. ExPASy TrEMBL
Match: A0A1S3CMG9 (uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=4 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 4.5e-290
Identity = 520/597 (87.10%), Postives = 561/597 (93.97%), Query Frame = 0

Query: 1   MIVAAGSSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTL 60
           MIVAAG  SNGGI SGELA+FI QVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIK TL
Sbjct: 1   MIVAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIV 120
           QCDQTTLN+IGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNF+GYFKIWQAVTGKIV
Sbjct: 61  QCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIV 120

Query: 121 QPTVAFFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180
           +PTVAFFCFYIM+GANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGHDTKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTV 240
           AIYGHDTKSL+LLIAWFPSIVSLIFVYTIREIK VKHPNEFRVFVQFLCVTVLLA+ LT 
Sbjct: 181 AIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTA 240

Query: 241 LVFVQNRVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIEN 300
           L+FVQ RV F QSAHIAIV+AILALL VPLLIAIREE+++WNLNKRT  +NPF RI IE+
Sbjct: 241 LIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIES 300

Query: 301 NPQTTTPIPSSN------QTQFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIG 360
           + Q   PI S +      QTQ +SCF+NIFNKPERGEDYTVLQAIFSIDMLI+CSTMLIG
Sbjct: 301 S-QPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIG 360

Query: 361 VGASLTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPL 420
           VGASLTAIDNLGQIGE+QGYPSETIN F+SL+SI NF+GRIFSGFVSEILLEKF+FPRPL
Sbjct: 361 VGASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPL 420

Query: 421 MLTLILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFN 480
           MLTLILLIS +GHLLVAFPF+DSLY+ASIIIGFS+GSQVPLHFAMISEIFGLKHYSTLFN
Sbjct: 421 MLTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFN 480

Query: 481 FGQLSCPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMI 540
           FGQLSCPIGSYILNVMVTGK+YD+VA++GSNPNK HC G+HCYEQSFLIL GLTFIVAMI
Sbjct: 481 FGQLSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMI 540

Query: 541 SLILVMRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQR 592
           SLILV RTR+FYRGDIYKKF+EDME+LKTEVEFY+VDEKRTRIGNLLVDK++INF++
Sbjct: 541 SLILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 596

BLAST of CcUC03G044850.1 vs. ExPASy TrEMBL
Match: A0A5D3BP48 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2042G00080 PE=4 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.1e-288
Identity = 517/595 (86.89%), Postives = 559/595 (93.95%), Query Frame = 0

Query: 3   VAAGSSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQC 62
           +AAG  SNGGI SGELA+FI QVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIK TLQC
Sbjct: 28  LAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQC 87

Query: 63  DQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIVQP 122
           DQTTLN+IGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNF+GYFKIWQAVTGKIV+P
Sbjct: 88  DQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRP 147

Query: 123 TVAFFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAI 182
           TVAFFCFYIM+GANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAI
Sbjct: 148 TVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAI 207

Query: 183 YGHDTKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLV 242
           YGHDTKSL+LLIAWFPSIVSLIFVYTIREIK VKHPNEFRVFVQFLCVTVLLA+ LT L+
Sbjct: 208 YGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALI 267

Query: 243 FVQNRVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNP 302
           FVQ RV F QSAHIAIV+AILALL VPLLIAIREE+++WNLNKRT  +NPF RI IE++ 
Sbjct: 268 FVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESS- 327

Query: 303 QTTTPIPSSN------QTQFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVG 362
           Q   PI S +      QTQ +SCF+NIFNKPERGEDYTVLQAIFSIDMLI+CSTMLIGVG
Sbjct: 328 QPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVG 387

Query: 363 ASLTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLML 422
           ASLTAIDNLGQIGE+QGYPSETIN F+SL+SI NF+GRIFSGFVSEILLEKF+FPRPLML
Sbjct: 388 ASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLML 447

Query: 423 TLILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFG 482
           TLILLIS +GHLLVAFPF+DSLY+ASIIIGFS+GSQVPLHFAMISEIFGLKHYSTLFNFG
Sbjct: 448 TLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFG 507

Query: 483 QLSCPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMISL 542
           QLSCPIGSYILNVMVTGK+YD+VA++GSNPNK HC G+HCYEQSFLIL GLTFIVAMISL
Sbjct: 508 QLSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMISL 567

Query: 543 ILVMRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQR 592
           ILV RTR+FYRGDIYKKF+EDME+LKTEVEFY+VDEKRTRIGNLLVDK++INF++
Sbjct: 568 ILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 621

BLAST of CcUC03G044850.1 vs. ExPASy TrEMBL
Match: A0A6J1K965 (uncharacterized protein LOC111493395 OS=Cucurbita maxima OX=3661 GN=LOC111493395 PE=4 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 1.0e-257
Identity = 457/593 (77.07%), Postives = 529/593 (89.21%), Query Frame = 0

Query: 7   SSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQCDQTT 66
           SS NGGI   EL+ FI+QVV GRWFSLFASFL+M+GAGGVYLFA YS+DIK TL+CDQTT
Sbjct: 8   SSINGGICRAELSCFIQQVVTGRWFSLFASFLIMSGAGGVYLFASYSKDIKSTLKCDQTT 67

Query: 67  LNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIVQPTVAF 126
           LNKIGFYKDLGSNVGVIAGLLAEVAPTW V+LIGAA NF+GYFKIWQAVTGKI++PTV F
Sbjct: 68  LNKIGFYKDLGSNVGVIAGLLAEVAPTWFVILIGAALNFVGYFKIWQAVTGKIIRPTVEF 127

Query: 127 FCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHD 186
           FCFYIMVGANSQNFANT VLV+CVKNFPERRGVMLGLLKGFVGLSGAIMTQ+Y A Y HD
Sbjct: 128 FCFYIMVGANSQNFANTVVLVSCVKNFPERRGVMLGLLKGFVGLSGAIMTQIYGAFYDHD 187

Query: 187 TKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLVFVQN 246
           TKSL+LL+AWFPS++S+IFV TIRE++AVKHPNEFRVFVQFLCVT++LA+FLT L+FVQ 
Sbjct: 188 TKSLILLLAWFPSLISIIFVKTIREMRAVKHPNEFRVFVQFLCVTIILALFLTALIFVQK 247

Query: 247 RVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNPQTTT 306
           +V+F+Q+AHI IV+AILALL+VPLLIAIREE+++WNLNKRT+  N FTRIRI+ NPQ+  
Sbjct: 248 KVQFNQTAHITIVAAILALLLVPLLIAIREEIIMWNLNKRTV-GNRFTRIRID-NPQSAP 307

Query: 307 PIPSSNQT--------QFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVGAS 366
              +S  +        Q +SCFANIFNKP+RGED+T+ QAIFS+DMLIL +TMLIGVGA+
Sbjct: 308 SCSASCSSSSVLELPKQSNSCFANIFNKPKRGEDFTIPQAIFSVDMLILLTTMLIGVGAN 367

Query: 367 LTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLMLTL 426
           LTAIDNLGQIGES  YP ETINSFISLVSIFNF+GRIFSGFVSEILLEKFKFPRP+MLTL
Sbjct: 368 LTAIDNLGQIGESLNYPPETINSFISLVSIFNFTGRIFSGFVSEILLEKFKFPRPMMLTL 427

Query: 427 ILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFGQL 486
           I L+SC+G+L+VAFPF +SL +ASIIIGFSLGSQVPLHFAMISE FGLKHYSTLFNFGQL
Sbjct: 428 IHLVSCVGYLIVAFPFNNSLCIASIIIGFSLGSQVPLHFAMISEFFGLKHYSTLFNFGQL 487

Query: 487 SCPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMISLIL 546
           SCPIGSYILNVMV GK+YD++A  G+N ++FHC GS C+EQSF IL GLTF+VAM+SL+L
Sbjct: 488 SCPIGSYILNVMVIGKLYDEMALTGNNASEFHCEGSKCFEQSFTILAGLTFVVAMVSLVL 547

Query: 547 VMRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQR 592
           V RT +FYRGDIY+KFREDM+SLKTE+EFY ++ KR +IGNL  DK+ INF++
Sbjct: 548 VRRTAEFYRGDIYQKFREDMDSLKTEMEFYPLESKRMKIGNLTFDKHAINFKK 598

BLAST of CcUC03G044850.1 vs. ExPASy TrEMBL
Match: A0A6J1GAT5 (uncharacterized protein LOC111452462 OS=Cucurbita moschata OX=3662 GN=LOC111452462 PE=4 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 1.0e-257
Identity = 458/593 (77.23%), Postives = 527/593 (88.87%), Query Frame = 0

Query: 7   SSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQCDQTT 66
           SS NGGI   EL+ FI+QVV+GRWFSLFASFL+M+GAGGVYLFA YS+DIK TL+CDQTT
Sbjct: 8   SSINGGICRAELSCFIQQVVMGRWFSLFASFLIMSGAGGVYLFASYSKDIKSTLKCDQTT 67

Query: 67  LNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIVQPTVAF 126
           LNKIGFYKDLGSNVGVIAGLLAEVAP W V+LIGAA NF+GYFKIWQAVTGKI++PTV F
Sbjct: 68  LNKIGFYKDLGSNVGVIAGLLAEVAPPWFVILIGAALNFVGYFKIWQAVTGKIIRPTVEF 127

Query: 127 FCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHD 186
           FCFYIMVGANSQNFANT VLV+CVKNFPERRGVMLGLLKGFVGLSGAIMTQ+Y AIY HD
Sbjct: 128 FCFYIMVGANSQNFANTVVLVSCVKNFPERRGVMLGLLKGFVGLSGAIMTQIYGAIYDHD 187

Query: 187 TKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLVFVQN 246
           TKSL+LL+AWFPS++S++FV TIRE++AVKHPNEFRVFVQFLCVTV+LA+FLT L+FVQ 
Sbjct: 188 TKSLILLLAWFPSLISIVFVKTIREMRAVKHPNEFRVFVQFLCVTVILALFLTALIFVQK 247

Query: 247 RVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNPQTTT 306
           +V F+Q+AHI IV+AI ALL+VPLLIAIREE+++WNLNKRT+  N FTRIRI+ NPQ+  
Sbjct: 248 KVPFNQTAHITIVAAIFALLLVPLLIAIREEIIMWNLNKRTV-GNRFTRIRID-NPQSAP 307

Query: 307 PIPSS--------NQTQFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVGAS 366
              +S           Q +SCFA IFNKP+RGED+T+ QAIFS+DMLIL +TMLIGVGA+
Sbjct: 308 SCSASCSSSSVLEQPKQSNSCFAAIFNKPKRGEDFTIPQAIFSVDMLILLTTMLIGVGAN 367

Query: 367 LTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLMLTL 426
           LTAIDNLGQIGES  YP ETINSFISLVSIFNF+GRIFSGFVSEILLEKFKFPRP+MLTL
Sbjct: 368 LTAIDNLGQIGESLNYPPETINSFISLVSIFNFTGRIFSGFVSEILLEKFKFPRPMMLTL 427

Query: 427 ILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFGQL 486
           ILL+SC+G+L+VAFPF +SL +ASIIIGFSLGSQVPLHFAMISE FGLKHYSTLFNFGQL
Sbjct: 428 ILLVSCVGYLIVAFPFNNSLCIASIIIGFSLGSQVPLHFAMISEFFGLKHYSTLFNFGQL 487

Query: 487 SCPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMISLIL 546
           SCPIGSYILNVMV GK+YD++A  GSN +KFHC GS C+EQSF IL GLTF+VAM+SL+L
Sbjct: 488 SCPIGSYILNVMVIGKLYDEMAMTGSNASKFHCEGSKCFEQSFTILAGLTFVVAMVSLVL 547

Query: 547 VMRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQR 592
           V RT +FYRGDIY+KFREDM+SLKTE+EFY ++ KR +IGNL  DK+ INF++
Sbjct: 548 VRRTAEFYRGDIYQKFREDMDSLKTEMEFYPLESKRMKIGNLTFDKHAINFKK 598

BLAST of CcUC03G044850.1 vs. ExPASy TrEMBL
Match: A0A6J1HYZ1 (uncharacterized protein LOC111469392 OS=Cucurbita maxima OX=3661 GN=LOC111469392 PE=4 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 8.7e-257
Identity = 459/591 (77.66%), Postives = 516/591 (87.31%), Query Frame = 0

Query: 1   MIVAAGSSSNGGIGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTL 60
           MIV+A  SSN GIGSGEL +F+RQVV GRWFSLFASFLVM GAGGVYLFAYYS DIK TL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIV 120
           QCDQTTLNKIGFYKDLGSNVG++AGLLAEVAPTW++LLIGAA NF+GYFKIWQ VTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 QPTVAFFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180
            PTVA+FCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGL KGFVGLSGAIMTQ+Y 
Sbjct: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGHDTKSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTV 240
           AIYG DT+SL+LL+ W PS++SLIF+ TIREIKAVKHPNEFRVFV FLCV+V+LA+FL  
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LVFVQNRVRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIEN 300
           L+F+Q R+RF QSAH A+V+AI ALL++PLLIAIREE++LWNLNKRTI  +P        
Sbjct: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHP-------- 300

Query: 301 NPQTT-TPIPSSNQTQFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVGASL 360
            PQ +  P PSS+            NKP RGED+T+ QAIFS DMLILC TMLIGVGASL
Sbjct: 301 -PQASPNPPPSSS------------NKPSRGEDHTIPQAIFSADMLILCITMLIGVGASL 360

Query: 361 TAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLMLTLI 420
           TAIDNL QIGES GYPSETINS I LVSIFNF+GRIFSGFVS+ILLEKFKFPRPLMLTL+
Sbjct: 361 TAIDNLRQIGESHGYPSETINSLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLV 420

Query: 421 LLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFGQLS 480
           LL+SCIGHLLVAFPF  SLY+ASI+IGFSLG+QVPLHFAMISE+FGLKHYSTLFNFGQLS
Sbjct: 421 LLVSCIGHLLVAFPFHGSLYIASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLS 480

Query: 481 CPIGSYILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMISLILV 540
           CPIGSYILNVMVTG++YD++A+  S     HC G  CYEQSF+IL GLTF VA++SLILV
Sbjct: 481 CPIGSYILNVMVTGRLYDEMAKATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILV 540

Query: 541 MRTRDFYRGDIYKKFREDMESLKTEVEFYKVDEKRTRIGNLLVDKYTINFQ 591
            RTR+FYRGDIYKKFREDMESLKT++EFY +D+KRTRIGNLLVDK++INF+
Sbjct: 541 GRTREFYRGDIYKKFREDMESLKTKMEFYTLDDKRTRIGNLLVDKHSINFK 570

BLAST of CcUC03G044850.1 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 623.6 bits (1607), Expect = 1.6e-178
Identity = 322/562 (57.30%), Postives = 414/562 (73.67%), Query Frame = 0

Query: 13  IGSGELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQCDQTTLNKIGF 72
           +G+ E   F+     GRWF +FASFL+M  AG  YLF  YS+DIK TL  DQTTLN +GF
Sbjct: 1   MGNDETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGF 60

Query: 73  YKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIVQPTVAFFCFYIM 132
           +KDLG+NVGV++GL+AEV PTW VL IG+A NF+GYF IW  VTGK+ +P V   C YI 
Sbjct: 61  FKDLGANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYIC 120

Query: 133 VGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTKSLVL 192
           +GANSQNFANTG LVTCVKNFPE RGVMLGLLKG+VGLSGAI TQLY AIYGHD+KSL+L
Sbjct: 121 IGANSQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLIL 180

Query: 193 LIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLVFVQNRVRFHQ 252
           LIAW P+ VSL+FVY IRE K V+  NE  VF QFL +++ LA+FL  +   + +V F +
Sbjct: 181 LIAWLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSK 240

Query: 253 SAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIE------NNPQTTT 312
           +A+ A  +   ALL VPL +++++EL +WN+ K  +P    + +++E      +  Q   
Sbjct: 241 AAYAASATICCALLFVPLTVSVKQELEVWNMMK--LPIEEPSEVKVEKPKKELDLDQDKA 300

Query: 313 PIPSSNQTQFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVGASLTAIDNLG 372
              +  + +  SCF+ +F+ P RGEDYT+LQA+ S DM+IL      G+G+SLTA+DNLG
Sbjct: 301 AKVNGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLG 360

Query: 373 QIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLMLTLILLISCIG 432
           QIGES GYP+ T++SF+SLVSI+N+ GR+FSGFVSE LL K+K PRPLM+TL+LL+SC G
Sbjct: 361 QIGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAG 420

Query: 433 HLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIGSYI 492
           HLL+AFP   S+Y+ASI++GFS G+Q+PL FA+ISE+FGLK+YSTLFN GQL+ P+GSYI
Sbjct: 421 HLLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYI 480

Query: 493 LNVMVTGKMYDKVA--QLGS------NPNKFHCAGSHCYEQSFLILGGLTFIVAMISLIL 552
           LNV VTG +YDK A  QL +      +     C GS CY+  FLIL  +TF  A++SL L
Sbjct: 481 LNVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGL 540

Query: 553 VMRTRDFYRGDIYKKFREDMES 561
            +RTR+FY+GDIYKKFRE  ES
Sbjct: 541 AIRTREFYKGDIYKKFRESPES 560

BLAST of CcUC03G044850.1 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 554.7 bits (1428), Expect = 9.1e-158
Identity = 295/569 (51.85%), Postives = 390/569 (68.54%), Query Frame = 0

Query: 16  GELANFIRQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQCDQTTLNKIGFYKD 75
           G + +   Q++ GRWF  F S L+M+ AG  Y+F  YS DIK+TL  DQTTLN + F+KD
Sbjct: 8   GSMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKD 67

Query: 76  LGSNVGVIAGLLAEVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIVQPTVAFFCFYIMVGA 135
           LG+NVGV+AGLL EV P W +LLIGA  NF GYF IW AVT +I +P V   C YI VGA
Sbjct: 68  LGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGA 127

Query: 136 NSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTKSLVLLIA 195
           NSQ+FANTG LVTCVKNFPE RGV+LG+LKG+VGLSGAI+TQLY A YG DTK L+L+I 
Sbjct: 128 NSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIG 187

Query: 196 WFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLVFVQNRVRFHQSAH 255
           W P+IVS  F+ TIR +K  +  NE +VF  FL +++ LA FL V++ +     F QS  
Sbjct: 188 WLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 247

Query: 256 IAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNPQTTTPIPSSNQTQ 315
               + ++ LL++P+++ I EE  LW   K+    +P     +   P+  +     +  +
Sbjct: 248 GGSAAVVIVLLLLPIIVVILEEKKLWK-EKQVALNDPAPINVVTEKPKLDSSEFKDDDGE 307

Query: 316 FS----------SCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVGASLTAIDNL 375
            S          SC+  +FN PERG+DYT+LQA+FS+DMLIL    + GVG +LTAIDNL
Sbjct: 308 ESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNL 367

Query: 376 GQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLMLTLILLISCI 435
           GQIG S GYP  ++++F+SLVSI+N+ GR+ SG VSEI L K+KFPRPLMLT++LL+SC 
Sbjct: 368 GQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCA 427

Query: 436 GHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIGSY 495
           GHLL+AF     LYVAS+IIGF  G+Q PL FA+ISEIFGLK+YSTL+NFG ++ PIGSY
Sbjct: 428 GHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSY 487

Query: 496 ILNVMVTGKMYDKVA-----QLGS---NPNKFHCAGSHCYEQSFLILGGLTFIVAMISLI 555
           +LNV V G +YD  A      LG         +C G+ C++ SF+I+  +T    ++S++
Sbjct: 488 LLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMV 547

Query: 556 LVMRTRDFYRGDIYKKFREDMESLKTEVE 567
           LV+RT+ FY+ DIYKKFRE  ++L  E+E
Sbjct: 548 LVIRTKKFYKSDIYKKFRE--KALAAEME 573

BLAST of CcUC03G044850.1 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 2.5e-83
Identity = 193/548 (35.22%), Postives = 311/548 (56.75%), Query Frame = 0

Query: 24  QVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQCDQTTLNKIGFYKDLGSNVGVI 83
           +++  +W ++ AS  +   +G  Y F  YS  +K T   DQ+TL+ +  +KD+G+N GV 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 84  AGLLAEVAPT----------------WLVLLIGAAFNFLGYFKIWQAVTGKIVQPTVAFF 143
           +GLL   A +                W+VL +GA   F GYF IW +VTG I +P V   
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 144 CFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDT 203
           C ++ + A SQ F NT  +V+ V+NF +  G  +G++KGF+GLSGAI+ QLY  +   D 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 204 KSLVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLVFVQNR 263
            S +LL+A  P+++SL+ +  +R I      ++ +       V++++A +L +++ ++N 
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNT 241

Query: 264 VRFHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNPQTTTP 323
                 A+I  +  +L +L +PLLIA R +    +  ++T+P +    I   ++P+ TT 
Sbjct: 242 FGLSSWANIVTLVCLLVMLALPLLIARRAQR---DGMEKTVPHDYSPLI---SSPKATT- 301

Query: 324 IPSSNQTQFSSCFANIFNKPERG--EDYTVLQAIFSIDMLILCSTMLIGVGASLTAIDNL 383
             S NQ+          +K E G  E+  +LQA+  +   +L   M+ G+G+ L+ I+N+
Sbjct: 302 --SGNQSSEGD------SKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNI 361

Query: 384 GQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLMLTLILLISCI 443
            QIGES  Y S  INS +SL SI+NF GR  +G+ S+ LL K  +PRPL++   L    I
Sbjct: 362 RQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSI 421

Query: 444 GHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIGSY 503
           GHL++A  F+ +LYV S+I+G   GSQ  L   + SE+FG++H  T+FN   ++ PIGSY
Sbjct: 422 GHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSY 481

Query: 504 ILNVMVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMISLILVMRTRDF 554
           I +V + G +YDK A    N     C GSHC+  SF+I+  + F   +++++L  RT+  
Sbjct: 482 IFSVRLIGYIYDKTASGEGNT----CYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTL 529

BLAST of CcUC03G044850.1 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 6.5e-79
Identity = 186/566 (32.86%), Postives = 298/566 (52.65%), Query Frame = 0

Query: 29  RWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQCDQTTLNKIGFYKDLGSNVGVIAGLLA 88
           RW    A+  + + AG  YLF   S  IK +L  +Q  L+++G  KDLG +VG IAG L+
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 89  EVAPTWLVLLIGAAFNFLGYFKIWQAVTGKIVQPTVAFFCFYIMVGANSQNFANTGVLVT 148
           E+ P W  LL+GA  N +GY  +W  VTG+     +   C  I VG N + + NTG LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 149 CVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTKSLVLLIAWFPSIVSLIFVYT 208
            V+NFP+ RG ++G+LKGF GL GAI++Q+Y  I+  +  SL+L++A  P++V +  ++ 
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFF 195

Query: 209 IREIKAVK--HPNEFRVFVQFLCVTVLLAIFLTVLVFVQNRVRFHQSAHIAIVSAILALL 268
           IR +   K   P +   F     V +LLA +L  ++ +Q+ V    +        +  +L
Sbjct: 196 IRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVIL 255

Query: 269 IVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNPQTTTP-----------------IP 328
           +VP+L+ I                 P    R +  P   TP                 +P
Sbjct: 256 VVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLLP 315

Query: 329 SSN--------QTQFSSCFA------NIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGV 388
           +S         Q Q     A      N    P RGED+T+ QA+   D  ++  ++L+G 
Sbjct: 316 ASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLGS 375

Query: 389 GASLTAIDNLGQIGESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLM 448
           G+ LT IDNLGQ+ +S GY  +  +  +S++SI+NF GRI  G+ SE+++  + +PRP+ 
Sbjct: 376 GSGLTVIDNLGQMSQSLGY--DNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYPRPVA 435

Query: 449 LTLILLISCIGHLLVAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNF 508
           + +  LI  +GH+  A+ +  ++Y+ +++IG   G+   +  A  SE+FGLK +  L+NF
Sbjct: 436 MAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYNF 495

Query: 509 GQLSCPIGSYILNVMVTGKMYDKVAQLGSNPNKF------HCAGSHCYEQSFLILGGLTF 556
             L+ P GS + + M+   +YD+ A+  ++ + F       C GS C+  + LI+ G   
Sbjct: 496 LTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMSGFCI 555

BLAST of CcUC03G044850.1 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 291.6 bits (745), Expect = 1.4e-78
Identity = 177/544 (32.54%), Postives = 303/544 (55.70%), Query Frame = 0

Query: 24  QVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKKTLQCDQTTLNKIGFYKDLGSNVGVI 83
           +++  +W ++ AS  +   AGG Y F  YS  +K T   DQ+TL+ +  +KD+G NVGV+
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 84  AGLLAEVAP--------------TWLVLLIGAAFNFLGYFKIWQAVTGKIVQPTVAFFCF 143
           +GL+   A                W+V+LIGA  NF GYF +W +VTG I +P V   C 
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 144 YIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTKS 203
           ++ + A S  F NT  +V+ ++NF +  G  +G++KGFVGLSGA++ QLY  +   D K+
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 204 LVLLIAWFPSIVSLIFVYTIREIKAVKHPNEFRVFVQFLCVTVLLAIFLTVLVFVQNRVR 263
            +LL+A  PS++S++ +  +R  K     +E +       +++++A +L + + +++ + 
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYK-TSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLS 243

Query: 264 FHQSAHIAIVSAILALLIVPLLIAIREELLLWNLNKRTIPTNPFTRIRIENNPQTTTPIP 323
               A+   ++ +L LL  PLL+A+R          R     P + +         +P+ 
Sbjct: 244 LPSWANAVTLAVLLVLLSSPLLVAVRAH--------RDSIEKPLSSV--------YSPLV 303

Query: 324 SSNQTQFSSCFANIFNKPERGEDYTVLQAIFSIDMLILCSTMLIGVGASLTAIDNLGQIG 383
            + +   S     +    +  +   +LQA+ ++D  +L   M+ G+G+ ++ I+N+ QIG
Sbjct: 304 DNLEATTSGEILML----DEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIG 363

Query: 384 ESQGYPSETINSFISLVSIFNFSGRIFSGFVSEILLEKFKFPRPLMLTLILLISCIGHLL 443
           ES  Y S  INS ++L +I+NF GR   G+VS+ LL +  +PRPL++   L    IGHL+
Sbjct: 364 ESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLI 423

Query: 444 VAFPFEDSLYVASIIIGFSLGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIGSYILNV 503
           +A  F+ +LY  SII+G   GSQ  L   + SE+FG+KH  T++N   ++ P+GSYI +V
Sbjct: 424 IASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSV 483

Query: 504 MVTGKMYDKVAQLGSNPNKFHCAGSHCYEQSFLILGGLTFIVAMISLILVMRTRDFYRGD 554
            + G +YD+      N     C G HC+  +++++  + F+  ++S +LV RT+  YR  
Sbjct: 484 RLIGYIYDRTIIGEGNT----CYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYR-Q 521

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895750.12.7e-29788.02uncharacterized protein LOC120083915 [Benincasa hispida][more]
XP_008464809.19.4e-29087.10PREDICTED: uncharacterized protein LOC103502605 [Cucumis melo][more]
KAA0051898.12.3e-28886.89protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ99888.1 protei... [more]
XP_004147303.34.1e-28583.36uncharacterized protein LOC101202941 [Cucumis sativus] >KGN64782.2 hypothetical ... [more]
XP_022998867.12.1e-25777.07uncharacterized protein LOC111493395 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
F4I9E16.2e-2624.13Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CMG94.5e-29087.10uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=... [more]
A0A5D3BP481.1e-28886.89Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1K9651.0e-25777.07uncharacterized protein LOC111493395 OS=Cucurbita maxima OX=3661 GN=LOC111493395... [more]
A0A6J1GAT51.0e-25777.23uncharacterized protein LOC111452462 OS=Cucurbita moschata OX=3662 GN=LOC1114524... [more]
A0A6J1HYZ18.7e-25777.66uncharacterized protein LOC111469392 OS=Cucurbita maxima OX=3661 GN=LOC111469392... [more]
Match NameE-valueIdentityDescription
AT2G28120.11.6e-17857.30Major facilitator superfamily protein [more]
AT2G39210.19.1e-15851.85Major facilitator superfamily protein [more]
AT1G74780.12.5e-8335.22Nodulin-like / Major Facilitator Superfamily protein [more]
AT5G14120.16.5e-7932.86Major facilitator superfamily protein [more]
AT1G18940.11.4e-7832.54Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 551..571
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 14..562
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 14..562
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 28..532
e-value: 6.98203E-93
score: 288.766
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 29..274
e-value: 3.7E-80
score: 269.0
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 317..545
e-value: 5.0E-12
score: 47.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 33..547

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CcUC03G044850CcUC03G044850gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC03G044850.1-exonCcUC03G044850.1-exon-CicolChr03:2817788..2819133exon
CcUC03G044850.1-exonCcUC03G044850.1-exon-CicolChr03:2819417..2819846exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC03G044850.1-cdsCcUC03G044850.1-cds-CicolChr03:2817788..2819133CDS
CcUC03G044850.1-cdsCcUC03G044850.1-cds-CicolChr03:2819417..2819846CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CcUC03G044850.1CcUC03G044850.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity