Carg27318-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg27318-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein of unknown function (DUF288)
LocationCarg_Chr17: 7614001 .. 7616684 (+)
Sequence length1650
RNA-Seq ExpressionCarg27318-RA
SyntenyCarg27318-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGCCTCCTTCTTCTTTCTCAAGGAGCTCCTGATTCTGCTGCATTTCTCATGCTTGCAACTCTCTCGCCCAAACCCCTGACCTCTTTCATTTGCCCAATATCAACTTTGATTACCTGTTCATCCCATTGTAGACAAGTCTTCGACTTACGCCCTTTATTAGCCTCTGATCGATGGATTGTTGTCTCCGTTTCGGTATTATCCTTCCCACATTCACCTGCGAAAGCTTGCGTAAAACTGAGATGGCAGGTACTAGCTGTTGGAAATTCTAGACGATCCATCGGATGGAGTCTCAGGGAGTTATATTTCTGTCTCATAGAGCAGCAATCTAGCTTCAGATTTAGAGTTGTGGATTTTTCTTCCTTAATGAATTCTTATGCTAGAAAGGACTTGTTGTGTACCTTTTCGGCTATCCAACATGGAAGCGGAAAAATGATATTCGATTGCAGATGATCGGGGCGAAGTGATTGATGGGGATCTCGGGAAGCATTTTGATTTGAAATTGTCCAATGTGATACATTTGCAAGAGAGAATCTTGCAGTTTGATTTTGAGAACCCGAATAAAACCGTTGTGAATCCGTATATTCATTCCGGACAGCGGTCCGAGGTTTGGCCTAGAGGGTTGCATTGGAGAATGTAGGAGATGTTGTGTATGAAGAACACACTACAGCCAAAGTATTTGGAGGATTACAGTCATTCACAGGGCATATCCAATGGTCTGCCAGATGTGGATTCAGTGTTTTACTTCACTCGAAAGACACGTTCGGAGGCATTGAACATAAGATTCGATGAGCACGCCCCGAAAGTTGCTCTGCCTCATGGCGTGATGGTACCATTGAATTCTTTCAATACTTGTTTCATACGTCAGCATTATGGGCTCTAATGCTTCCCGCTTCAGTTAGTTACAATGGCTTCTGATATCCTGAGGGGTTATGGGGCACAGAGGCTTTTATGGGAAGTAGGAGGTTTTGTAGTGGGTTTATCCACCAACAATGTTTAGACATGATGACATTGAAGGATATCCATTTTCAGAAGAGAAGATCTCCATGTGAATGTGGGCAGATTGGTAAAGTTCTTGACCTCATGGAGATCAAACAAGGCCACATTCTTTGAGAAGGCTTTGGAATTGAGCCATTCAATGGCAGAGGAAGGGTTTTGGAAGGACAATGACGTGAAACTGAGTGTGGCTTGGCTTCAAGATTTGGTTTCTGTTGGGTACATCCAACCAAGACTGAAGGGATTTGAAATGAACAAACAGAGAAGAGGAAGAAGAAGCAATGTCGGTGTCGGTGTCGGTGTCGATAATGGGAGGAGTTTTTGTTCCTCAAAAACTGCCTGGGTTTTCATCTGGGGTGGAGGAATCTGAGACTGTGAACTTCGAGATAGGGAAGTTGGTTAGGTGGAGGAAGAAGTTTGGCAATGTGGTGGATGATCTTGTTCGTTGAAAATGGAGGTGTTGTGGATAGAACTGCCATGAAATGGAGATTGCTTTTATGGAAGGATTTTCAAAACTGTGGGTGGTTGTGGCAGAGCATGGCAGGGCAGATTGGGAGTGGAGGAAAGCTTCTTTGGAGTTCATATACAAGTAAATAACTCTTCCTATCCTTCAATATAATTACATTTTCAGTACTTTGTTCTCGGTATCTTTTTCAACTTGTAGTCTATTTTTTTTTAGGGGAAAATTGTTATTGTATCGTTTTTACTTTTTATTATCTTTTGCTTAACGGACGGGGAGGAGAACAAACACCACCATTTATAAGGTTGTGGAAAAAAACCTTCTCCTACCAGACGCGTTTTAAAAAGTCTTGAGGGGAAGCTCGAAAGAGAAAGCCAAAGAAGGACAATATCTGCTAGCCGTGGATCTGGGTCGTTACATGTTTAGTTCTTAATTAAAATGAAAAAATGACTAAATATTTATGTAGAAAAGGTCATAATTTCCTTTTATAGGTACCTGCCAACCGTCTTTGAAAGATTTCCTAATGCAGAAGGTTTCCTGTTCCTGCAAGATAATACCATTCTCAACTATTGGAATTTACTGCAAGCAGATAAAGATAAGCTTTGGATCACTTACAAGGTAATGCAGGTCTCAGTTGTATCTCTCTCTTGCTCCTTAGATCAGTTGAAAAACGTATGAATATTTCTGTTTCTCACAGGTCCCTCAGTCTTGGTCCAGAGTCAGTGATGACTCTGTCTGGTTTGGTAAACAAGCAAGCTGGGTGAAGAAGGTTGTGAGCACAATGCCTGTTGATTTTCAAGTCAACTATAAGGAAAGTAACCCGACTGCGCGATATCTCGTGATTTGTAACTGTGAAGTGTTTTACGTACCTCGGCAGTTTGTGGGAGACTTCAAGGATCTTGTGGCTCTTGTTGGCAACTACAAGATTGACTACAGAGTAGCTGTGCCAATGTTCTTCATGGCAATGGATCTGCCCCTAAATTTCGACGACGTGTTCGGCCGAATGGTGTATAAGAAGACACCAGCTGAGCAACTGATAAGTAATGATACAAACTTGTATGCTGCTGAAGTTCCTGCTGTTCATCCATGGAGGGTTTCCAGTGAAGTGGAATTTGCTGAGCTTATGAGACTCATGGCTGCAGGTGACCCGCTGCTGAAGGAGCTGGTGTAAGAGTTGAATATACTCAAAATATGAGTGTACAAATGTATGTGTAATTTGGAGCCATGT

mRNA sequence

ATGCGCCTCCTTCTTCTTTCTCAAGGAGCTCCTGATTCTGCTGCATTTCTCATGCTTGCAACTCTCTCGCCCAAACCCCTGACCTCTTTCATTTGCCCAATATCAACTTTGATTACCTGTTCATCCCATTGTAGACAAGTCTTCGACTTACGCCCTTTATTAGCCTCTGATCGATGGATTGTTGTCTCCGTTTCGGTATTATCCTTCCCACATTCACCTGCGAAAGCTTGCGTAAAACTGAGATGGCAGGTACTAGCTGTTGGAAATTCTAGACGATCCATCGGATGGAGTCTCAGGGAGTTATATTTCTATGATCGGGGCGAAGTGATTGATGGGGATCTCGGGAAGCATTTTGATTTGAAATTGTCCAATGTGATACATTTGCAAGAGAGAATCTTGCAGTTTGATTTTGAGAACCCGAATAAAACCGTTGTGAATCCGTATATTCATTCCGGACAGCGGTCCGAGGGCATATCCAATGGTCTGCCAGATGTGGATTCAGTGTTTTACTTCACTCGAAAGACACGTTCGGAGGCATTGAACATAAGATTCGATGAGCACGCCCCGAAAGTTGCTCTGCCTCATGGCGTGATGGGGTTATGGGGCACAGAGGCTTTTATGGGAAGTAGGAGGTTTTGTAGTGGATTGGTAAAGTTCTTGACCTCATGGAGATCAAACAAGGCCACATTCTTTGAGAAGGCTTTGGAATTGAGCCATTCAATGGCAGAGGAAGGGTTTTGGAAGGACAATGACGTGAAACTGAGTGTGGCTTGGCTTCAAGATTTGGTTTCTGTTGGAGAAGAGGAAGAAGAAGCAATGTCGGTGTCGGTGTCGGTGTCGATAATGGGAGGAGTTTTTGTTCCTCAAAAACTGCCTGGGTTTTCATCTGGGGTGGAGGAATCTGAGACTGTGAACTTCGAGATAGGGAAGTTGGTTAGGTGGAGGAAGAAGTTTGGCAATGTGGTGGATGATCTTAGCATGGCAGGGCAGATTGGGAGTGGAGGAAAGCTTCTTTGGAGTTCATATACAAAAGGTTTCCTGTTCCTGCAAGATAATACCATTCTCAACTATTGGAATTTACTGCAAGCAGATAAAGATAAGCTTTGGATCACTTACAAGGTCCCTCAGTCTTGGTCCAGAGTCAGTGATGACTCTGTCTGGTTTGGTAAACAAGCAAGCTGGGTGAAGAAGGTTGTGAGCACAATGCCTGTTGATTTTCAAGTCAACTATAAGGAAAGTAACCCGACTGCGCGATATCTCGTGATTTGTAACTGTGAAGTGTTTTACGTACCTCGGCAGTTTGTGGGAGACTTCAAGGATCTTGTGGCTCTTGTTGGCAACTACAAGATTGACTACAGAGTAGCTGTGCCAATGTTCTTCATGGCAATGGATCTGCCCCTAAATTTCGACGACGTGTTCGGCCGAATGGTGTATAAGAAGACACCAGCTGAGCAACTGATAAGTAATGATACAAACTTGTATGCTGCTGAAGTTCCTGCTGTTCATCCATGGAGGGTTTCCAGTGAAGTGGAATTTGCTGAGCTTATGAGACTCATGGCTGCAGGTGACCCGCTGCTGAAGGAGCTGGTGTAAGAGTTGAATATACTCAAAATATGAGTGTACAAATGTATGTGTAATTTGGAGCCATGT

Coding sequence (CDS)

ATGCGCCTCCTTCTTCTTTCTCAAGGAGCTCCTGATTCTGCTGCATTTCTCATGCTTGCAACTCTCTCGCCCAAACCCCTGACCTCTTTCATTTGCCCAATATCAACTTTGATTACCTGTTCATCCCATTGTAGACAAGTCTTCGACTTACGCCCTTTATTAGCCTCTGATCGATGGATTGTTGTCTCCGTTTCGGTATTATCCTTCCCACATTCACCTGCGAAAGCTTGCGTAAAACTGAGATGGCAGGTACTAGCTGTTGGAAATTCTAGACGATCCATCGGATGGAGTCTCAGGGAGTTATATTTCTATGATCGGGGCGAAGTGATTGATGGGGATCTCGGGAAGCATTTTGATTTGAAATTGTCCAATGTGATACATTTGCAAGAGAGAATCTTGCAGTTTGATTTTGAGAACCCGAATAAAACCGTTGTGAATCCGTATATTCATTCCGGACAGCGGTCCGAGGGCATATCCAATGGTCTGCCAGATGTGGATTCAGTGTTTTACTTCACTCGAAAGACACGTTCGGAGGCATTGAACATAAGATTCGATGAGCACGCCCCGAAAGTTGCTCTGCCTCATGGCGTGATGGGGTTATGGGGCACAGAGGCTTTTATGGGAAGTAGGAGGTTTTGTAGTGGATTGGTAAAGTTCTTGACCTCATGGAGATCAAACAAGGCCACATTCTTTGAGAAGGCTTTGGAATTGAGCCATTCAATGGCAGAGGAAGGGTTTTGGAAGGACAATGACGTGAAACTGAGTGTGGCTTGGCTTCAAGATTTGGTTTCTGTTGGAGAAGAGGAAGAAGAAGCAATGTCGGTGTCGGTGTCGGTGTCGATAATGGGAGGAGTTTTTGTTCCTCAAAAACTGCCTGGGTTTTCATCTGGGGTGGAGGAATCTGAGACTGTGAACTTCGAGATAGGGAAGTTGGTTAGGTGGAGGAAGAAGTTTGGCAATGTGGTGGATGATCTTAGCATGGCAGGGCAGATTGGGAGTGGAGGAAAGCTTCTTTGGAGTTCATATACAAAAGGTTTCCTGTTCCTGCAAGATAATACCATTCTCAACTATTGGAATTTACTGCAAGCAGATAAAGATAAGCTTTGGATCACTTACAAGGTCCCTCAGTCTTGGTCCAGAGTCAGTGATGACTCTGTCTGGTTTGGTAAACAAGCAAGCTGGGTGAAGAAGGTTGTGAGCACAATGCCTGTTGATTTTCAAGTCAACTATAAGGAAAGTAACCCGACTGCGCGATATCTCGTGATTTGTAACTGTGAAGTGTTTTACGTACCTCGGCAGTTTGTGGGAGACTTCAAGGATCTTGTGGCTCTTGTTGGCAACTACAAGATTGACTACAGAGTAGCTGTGCCAATGTTCTTCATGGCAATGGATCTGCCCCTAAATTTCGACGACGTGTTCGGCCGAATGGTGTATAAGAAGACACCAGCTGAGCAACTGATAAGTAATGATACAAACTTGTATGCTGCTGAAGTTCCTGCTGTTCATCCATGGAGGGTTTCCAGTGAAGTGGAATTTGCTGAGCTTATGAGACTCATGGCTGCAGGTGACCCGCTGCTGAAGGAGCTGGTGTAA

Protein sequence

MRLLLLSQGAPDSAAFLMLATLSPKPLTSFICPISTLITCSSHCRQVFDLRPLLASDRWIVVSVSVLSFPHSPAKACVKLRWQVLAVGNSRRSIGWSLRELYFYDRGEVIDGDLGKHFDLKLSNVIHLQERILQFDFENPNKTVVNPYIHSGQRSEGISNGLPDVDSVFYFTRKTRSEALNIRFDEHAPKVALPHGVMGLWGTEAFMGSRRFCSGLVKFLTSWRSNKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVSVSIMGGVFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNVVDDLSMAGQIGSGGKLLWSSYTKGFLFLQDNTILNYWNLLQADKDKLWITYKVPQSWSRVSDDSVWFGKQASWVKKVVSTMPVDFQVNYKESNPTARYLVICNCEVFYVPRQFVGDFKDLVALVGNYKIDYRVAVPMFFMAMDLPLNFDDVFGRMVYKKTPAEQLISNDTNLYAAEVPAVHPWRVSSEVEFAELMRLMAAGDPLLKELV
Homology
BLAST of Carg27318-RA vs. NCBI nr
Match: KAG7014260.1 (putative glycosyltransferase STELLO1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MRLLLLSQGAPDSAAFLMLATLSPKPLTSFICPISTLITCSSHCRQVFDLRPLLASDRWI 60
           MRLLLLSQGAPDSAAFLMLATLSPKPLTSFICPISTLITCSSHCRQVFDLRPLLASDRWI
Sbjct: 1   MRLLLLSQGAPDSAAFLMLATLSPKPLTSFICPISTLITCSSHCRQVFDLRPLLASDRWI 60

Query: 61  VVSVSVLSFPHSPAKACVKLRWQVLAVGNSRRSIGWSLRELYFYDRGEVIDGDLGKHFDL 120
           VVSVSVLSFPHSPAKACVKLRWQVLAVGNSRRSIGWSLRELYFYDRGEVIDGDLGKHFDL
Sbjct: 61  VVSVSVLSFPHSPAKACVKLRWQVLAVGNSRRSIGWSLRELYFYDRGEVIDGDLGKHFDL 120

Query: 121 KLSNVIHLQERILQFDFENPNKTVVNPYIHSGQRSEGISNGLPDVDSVFYFTRKTRSEAL 180
           KLSNVIHLQERILQFDFENPNKTVVNPYIHSGQRSEGISNGLPDVDSVFYFTRKTRSEAL
Sbjct: 121 KLSNVIHLQERILQFDFENPNKTVVNPYIHSGQRSEGISNGLPDVDSVFYFTRKTRSEAL 180

Query: 181 NIRFDEHAPKVALPHGVMGLWGTEAFMGSRRFCSGLVKFLTSWRSNKATFFEKALELSHS 240
           NIRFDEHAPKVALPHGVMGLWGTEAFMGSRRFCSGLVKFLTSWRSNKATFFEKALELSHS
Sbjct: 181 NIRFDEHAPKVALPHGVMGLWGTEAFMGSRRFCSGLVKFLTSWRSNKATFFEKALELSHS 240

Query: 241 MAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVSVSIMGGVFVPQKLPGFSSGVEE 300
           MAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVSVSIMGGVFVPQKLPGFSSGVEE
Sbjct: 241 MAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVSVSIMGGVFVPQKLPGFSSGVEE 300

Query: 301 SETVNFEIGKLVRWRKKFGNVVDDLSMAGQIGSGGKLLWSSYTKGFLFLQDNTILNYWNL 360
           SETVNFEIGKLVRWRKKFGNVVDDLSMAGQIGSGGKLLWSSYTKGFLFLQDNTILNYWNL
Sbjct: 301 SETVNFEIGKLVRWRKKFGNVVDDLSMAGQIGSGGKLLWSSYTKGFLFLQDNTILNYWNL 360

Query: 361 LQADKDKLWITYKVPQSWSRVSDDSVWFGKQASWVKKVVSTMPVDFQVNYKESNPTARYL 420
           LQADKDKLWITYKVPQSWSRVSDDSVWFGKQASWVKKVVSTMPVDFQVNYKESNPTARYL
Sbjct: 361 LQADKDKLWITYKVPQSWSRVSDDSVWFGKQASWVKKVVSTMPVDFQVNYKESNPTARYL 420

Query: 421 VICNCEVFYVPRQFVGDFKDLVALVGNYKIDYRVAVPMFFMAMDLPLNFDDVFGRMVYKK 480
           VICNCEVFYVPRQFVGDFKDLVALVGNYKIDYRVAVPMFFMAMDLPLNFDDVFGRMVYKK
Sbjct: 421 VICNCEVFYVPRQFVGDFKDLVALVGNYKIDYRVAVPMFFMAMDLPLNFDDVFGRMVYKK 480

Query: 481 TPAEQLISNDTNLYAAEVPAVHPWRVSSEVEFAELMRLMAAGDPLLKELV 531
           TPAEQLISNDTNLYAAEVPAVHPWRVSSEVEFAELMRLMAAGDPLLKELV
Sbjct: 481 TPAEQLISNDTNLYAAEVPAVHPWRVSSEVEFAELMRLMAAGDPLLKELV 530

BLAST of Carg27318-RA vs. NCBI nr
Match: KAG7014258.1 (putative glycosyltransferase STELLO1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 708.0 bits (1826), Expect = 6.2e-200
Identity = 416/705 (59.01%), Postives = 433/705 (61.42%), Query Frame = 0

Query: 8   QGAPDSAAFLMLATLSPKPLTSFICP------ISTLITCSSHCRQVFDLRPLLASDRWIV 67
           QGAPDSAAFL   +  PKP   F  P      +  ++  SS            +SDRWIV
Sbjct: 56  QGAPDSAAFLCFNS-RPKPPDLFHLPNINFDSVHPIVDKSSTYAS-------FSSDRWIV 115

Query: 68  VSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------------- 127
           VSVS  S+P    +   K R WQVLAVGNSR    WSL+ + F                 
Sbjct: 116 VSVS--SYPSDSLRKLAKTRGWQVLAVGNSRTPSDWSLKGVIFLSLEQQSSLGFRVVDFL 175

Query: 128 ---------------------------DRGEVIDGDLGKHFDLKLSNVIHLQERILQFDF 187
                                      DRGEVIDGDLGKHFDLKLSNV  LQERILQFDF
Sbjct: 176 PYDSYARKTVGYLFAIQHGAKMIFDADDRGEVIDGDLGKHFDLKLSNVDTLQERILQFDF 235

Query: 188 ENPNKTVVNPYIHSGQRS-------------------------------EGISNGLPDVD 247
           ENPNKTVVNPYIH GQRS                               +GISNGLPDVD
Sbjct: 236 ENPNKTVVNPYIHFGQRSVWPRGLPLENVGDVVYEEHYSQVFGGLQFIQQGISNGLPDVD 295

Query: 248 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------------- 307
           SVFYFTRKTRSE LNIRFDEHAPKVALPHGVM                            
Sbjct: 296 SVFYFTRKTRSETLNIRFDEHAPKVALPHGVMVPLNSFNTLFHTSALWALMLPASVSTMA 355

Query: 308 -----GLWGTEAFMGSRRF----------------------------CSGLVKFLTSWRS 367
                G W          F                               LVKFLTSWRS
Sbjct: 356 SDILRGYWAQRLLWEVGGFVVVYPPTMFRHDDIEGYPFSEEKDLHVNVGRLVKFLTSWRS 415

Query: 368 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG-------------EEEEEA 427
           NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG             +     
Sbjct: 416 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGYIQPRLKGFEMNKQRRGRR 475

Query: 428 MSVSVSVSIMGG-VFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNVV--------- 487
            +V V V +  G  FVPQKLPGF  GVEESETVNFEIGKLVRWRKKFGNVV         
Sbjct: 476 SNVGVGVGVDNGRSFVPQKLPGFHLGVEESETVNFEIGKLVRWRKKFGNVVMILFVENGG 535

Query: 488 -----------------DDLSMAGQIGSGGKLLWSSYTKGFLFLQDNTILNYWNLLQADK 531
                              +SMAGQI    KLLWSSYT       +NTILNYWNLLQADK
Sbjct: 536 VVDRTAMKWRLLYGRIFKTVSMAGQIWEWRKLLWSSYT-------NNTILNYWNLLQADK 595

BLAST of Carg27318-RA vs. NCBI nr
Match: XP_022953811.1 (probable glycosyltransferase STELLO2 [Cucurbita moschata])

HSP 1 Score: 696.0 bits (1795), Expect = 2.4e-196
Identity = 412/718 (57.38%), Postives = 435/718 (60.58%), Query Frame = 0

Query: 8   QGAPDSAAFLMLATLSPKPLTSFICP------ISTLITCSSHCRQVFDLRPLLASDRWIV 67
           QGAPDSAAFL   +  PKP   F  P      +  ++  SS            +SDRWIV
Sbjct: 56  QGAPDSAAFLCFNS-RPKPPDLFHLPNINFDSVHPIVDKSSTYAS-------FSSDRWIV 115

Query: 68  VSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------------- 127
           VSVS  S+P    +   K R WQVLAVGNSR    WSL+ + F                 
Sbjct: 116 VSVS--SYPSDSLRKLAKTRGWQVLAVGNSRTPSDWSLKGVIFLSLEQQSSLGFRVVDFL 175

Query: 128 ---------------------------DRGEVIDGDLGKHFDLKLSNVIHLQERILQFDF 187
                                      DRGEVIDGDLGKHFDLKLSNV  LQERILQFDF
Sbjct: 176 PYDSYARKTVGYLFAIQHGAKMIFDADDRGEVIDGDLGKHFDLKLSNVDTLQERILQFDF 235

Query: 188 ENPNKTVVNPYIHSGQRS-------------------------------EGISNGLPDVD 247
           ENPNKTVVNPYIH GQRS                               +GISNGLPDVD
Sbjct: 236 ENPNKTVVNPYIHFGQRSVWPRGLPLENVGDVVYEEHYSQVFGGLQFIQQGISNGLPDVD 295

Query: 248 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------------- 307
           SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM                            
Sbjct: 296 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVMVPLNSFNTLFHTSALWALMLPASVSTMA 355

Query: 308 -----GLWGTEAFMGSRRF----------------------------CSGLVKFLTSWRS 367
                G W          F                               LVKFLTSWRS
Sbjct: 356 SDILRGYWAQRLLWEVGGFVVVYPPTMFRHDDIEGYPFSEEKDLHVNVGRLVKFLTSWRS 415

Query: 368 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG---------EEEEEAMSVS 427
           NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG         E  ++     
Sbjct: 416 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGYIQPRLKGFEMNKQRRGRR 475

Query: 428 VSVSIMGG-VFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNV-----VDDLSMAGQ 487
            +V +  G  FVPQKLPGF  GVEESETVNFEIGKLVRWRKKFGNV     V++  +  +
Sbjct: 476 SNVGVDNGRSFVPQKLPGFHLGVEESETVNFEIGKLVRWRKKFGNVVMILFVENGGVVDR 535

Query: 488 IGSGGKLLW--------------------------------------SSYTKGFLFLQDN 531
                +LL+                                      S   +GFLFLQDN
Sbjct: 536 TAMKWRLLYGRIFKTVVVVAEHGRADLGVEEASLEFIYKYLPTVFERSPNAEGFLFLQDN 595

BLAST of Carg27318-RA vs. NCBI nr
Match: KAG6575702.1 (putative glycosyltransferase STELLO1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 694.5 bits (1791), Expect = 7.1e-196
Identity = 412/722 (57.06%), Postives = 433/722 (59.97%), Query Frame = 0

Query: 8   QGAPDSAAFLMLATLSPKPLTSFICP------ISTLITCSSHCRQVFDLRPLLASDRWIV 67
           QGAPDSAAFL   +  PKP   F  P      +  ++  SS            +SDRWIV
Sbjct: 56  QGAPDSAAFLCFNS-RPKPPDLFHLPNINFDSVHPIVDKSSTYAS-------FSSDRWIV 115

Query: 68  VSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------------- 127
           VSVS  S+P    +   K R WQVLAVGNSR    WSL+ + F                 
Sbjct: 116 VSVS--SYPSDSLRKLPKTRGWQVLAVGNSRTPSDWSLKGVIFLSLEQQSSLGFRVVDFL 175

Query: 128 ---------------------------DRGEVIDGDLGKHFDLKLSNVIHLQERILQFDF 187
                                      DRGEVIDGDLGKHFDLKLSNV  LQERILQFDF
Sbjct: 176 PYDSYARKTVGYLFAIQHGAKMIFDADDRGEVIDGDLGKHFDLKLSNVDTLQERILQFDF 235

Query: 188 ENPNKTVVNPYIHSGQRS-------------------------------EGISNGLPDVD 247
           ENPNKTVVNPYIH GQRS                               +GISNGLPDVD
Sbjct: 236 ENPNKTVVNPYIHFGQRSVWPRGLPLENVGDVVYEEHYSQVFGGLQFIQQGISNGLPDVD 295

Query: 248 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------------- 307
           SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM                            
Sbjct: 296 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVMVPLNSFNTLFHTSALWALMLPASVSTMA 355

Query: 308 -----GLWGTEAFMGSRRF----------------------------CSGLVKFLTSWRS 367
                G W          F                               LVKFLTSWRS
Sbjct: 356 SDILRGYWAQRLLWEVGGFVVVYPPTMFRHDDIEGYPFSEEKDLHVNVGRLVKFLTSWRS 415

Query: 368 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG-------------EEEEEA 427
           NKATFFEKALELSHSM EEGFWKDNDVKLSVAWLQDLVSVG             +     
Sbjct: 416 NKATFFEKALELSHSMEEEGFWKDNDVKLSVAWLQDLVSVGYIQPRLKGFEMNKQRRGRR 475

Query: 428 MSVSVSVSIMGG-VFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNV-----VDDLS 487
            +V V V +  G  FVPQKLPGF  GVEESETVNFEIGKLVRWRKKFGNV     V++  
Sbjct: 476 SNVGVGVGVDNGRSFVPQKLPGFHLGVEESETVNFEIGKLVRWRKKFGNVVMILFVENGG 535

Query: 488 MAGQIGSGGKLLWSSYTK--------------------------------------GFLF 531
           +  +     +LL+    K                                      GFLF
Sbjct: 536 VVDRTAMKWRLLYGRIFKTVVVVAEHGRADLGVEEASLEFIYKYLPTVFERFPNAEGFLF 595

BLAST of Carg27318-RA vs. NCBI nr
Match: XP_022991857.1 (probable glycosyltransferase STELLO2 [Cucurbita maxima])

HSP 1 Score: 687.2 bits (1772), Expect = 1.1e-193
Identity = 407/717 (56.76%), Postives = 429/717 (59.83%), Query Frame = 0

Query: 8   QGAPDSAAFLMLATLSPKPLTSFICP------ISTLITCSSHCRQVFDLRPLLASDRWIV 67
           QGA DSAAFL   +  PKP   F  P      +  ++  SS            +SDRWIV
Sbjct: 56  QGARDSAAFLCFNS-RPKPPELFHLPKINFDSVHPIVDKSSSYAS-------FSSDRWIV 115

Query: 68  VSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------------- 127
           VSVS  S+P    +   K R WQVLAVGNSR    WSL+ + F                 
Sbjct: 116 VSVS--SYPSDSLRKLAKTRGWQVLAVGNSRTPSDWSLKGVIFLSLEQQSSLGFRVVDFL 175

Query: 128 ---------------------------DRGEVIDGDLGKHFDLKLSNVIHLQERILQFDF 187
                                      DR EVIDGDLGKHFDLKLSNV  LQERILQFDF
Sbjct: 176 PYDSYARKTVGYLFAIQHGAKMIFDADDRCEVIDGDLGKHFDLKLSNVDTLQERILQFDF 235

Query: 188 ENPNKTVVNPYIHSGQRS-------------------------------EGISNGLPDVD 247
           ENPNKTVVNPYIH GQRS                               +GISNGLPDVD
Sbjct: 236 ENPNKTVVNPYIHFGQRSVWPRGLPLENVGDVVYEEHYSQVFGGLQFIQQGISNGLPDVD 295

Query: 248 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------------- 307
           SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM                            
Sbjct: 296 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVMVPLNSFNTLFHTSALWALMLPASVSTMA 355

Query: 308 -----GLWGTEAFMGSRRF----------------------------CSGLVKFLTSWRS 367
                G W          F                               L+KFLTSWRS
Sbjct: 356 SDILRGYWAQRLLWELGGFVVVYPPTMFRHDDIEGYPFSEEKDLHVNVGRLIKFLTSWRS 415

Query: 368 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG---------EEEEEAMSVS 427
           NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG         E  ++    S
Sbjct: 416 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGYIQPRLKGFEMNKQRRRRS 475

Query: 428 VSVSIMGGVFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNV-----VDDLSMAGQI 487
                 G  FVPQKLPGF  GVEESETVNFEIGKLVRWRKKFGNV     V++  +  + 
Sbjct: 476 NGGGDTGRSFVPQKLPGFHLGVEESETVNFEIGKLVRWRKKFGNVVMILFVENGGVVDRT 535

Query: 488 GSGGKLLWSSYTK--------------------------------------GFLFLQDNT 531
               +LL+    K                                      GFLFLQDNT
Sbjct: 536 AMKWRLLYGRIFKTVVVVAENGRADLGVEEASLEFIYKYLPTVFERFPNAEGFLFLQDNT 595

BLAST of Carg27318-RA vs. ExPASy Swiss-Prot
Match: O22943 (Probable glycosyltransferase STELLO1 OS=Arabidopsis thaliana OX=3702 GN=STL1 PE=1 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 4.1e-122
Identity = 273/661 (41.30%), Postives = 347/661 (52.50%), Query Frame = 0

Query: 56  SDRWIVVSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------- 115
           +++WIVVSV+   +P    K+ VK+R WQVLA+GNS     WSL+   F           
Sbjct: 115 TEKWIVVSVT--KYPTEELKSLVKIRGWQVLAIGNSATPKDWSLKGSIFLSLDAQAELGY 174

Query: 116 ---------------------------------DRGEVIDGDLGKHFDLKLSNVIHLQER 175
                                            DRGEVIDGDLGKHFD++L  +   QE 
Sbjct: 175 RVLDHLPYDSFVRKSVGYLFAIQHGAKKIYDADDRGEVIDGDLGKHFDVELVGLDSKQEP 234

Query: 176 ILQFDFENPNKTVVNPYIHSGQRS-------------------------------EGISN 235
           ILQ+  ENPN+TVVNPYIH GQRS                               +GISN
Sbjct: 235 ILQYSHENPNRTVVNPYIHFGQRSVWPRGLPLENVGEINHEEYYTEVFGGKQFIQQGISN 294

Query: 236 GLPDVDSVFYFTRKTRSEALNIRFDEHAPKVALPHGVM-------GLWGTEAFMG----- 295
           GLPDVDSVFYFTRKT  EA +IRFDEH+PKVALP GVM        L+ + AF G     
Sbjct: 295 GLPDVDSVFYFTRKTTLEAFDIRFDEHSPKVALPQGVMVPVNSFNTLYHSSAFWGLMLPV 354

Query: 296 --------------------------------SRRF-----------------CSGLVKF 355
                                           + RF                    L+KF
Sbjct: 355 SVSSMASDVLRGYWGQRLLWELGGYVAVYPPTAHRFDRIEAYPFVEEKDLHVNVGRLIKF 414

Query: 356 LTSWRSNKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVS- 415
           L +WRS K +FFE  L+LS +MAEEGFW + D+K + AWLQDL++VG ++   MS+ +  
Sbjct: 415 LLAWRSEKHSFFETVLDLSFAMAEEGFWTEQDLKFTAAWLQDLIAVGYQQPRLMSLELDR 474

Query: 416 --VSIMGG---VFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNVVDDLSMAGQIGS 475
              SI  G    FVP+KLP    GVEE+ TV+ EIG L+RWRK FGNVV  +   G +  
Sbjct: 475 PRASIGHGDRKEFVPRKLPSVHLGVEETGTVSTEIGNLIRWRKNFGNVVLVMFCNGPVER 534

Query: 476 GG---KLLWS--------------------------------------SYTKGFLFLQDN 531
                +LL+                                       S  +GFLF++D+
Sbjct: 535 TALEWRLLYGRIFKTVVILSSQKNSDLYVEEAKLDHIYKHLPKIFDRYSSAEGFLFVEDD 594

BLAST of Carg27318-RA vs. ExPASy Swiss-Prot
Match: Q9SCN0 (Probable glycosyltransferase STELLO2 OS=Arabidopsis thaliana OX=3702 GN=STL2 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.5e-119
Identity = 271/662 (40.94%), Postives = 341/662 (51.51%), Query Frame = 0

Query: 56  SDRWIVVSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------- 115
           +++WIVVSV+    P    K  VK++ WQVLA+GNS     W+L+   F           
Sbjct: 108 TEKWIVVSVT--KHPTEELKGLVKIKGWQVLAIGNSLTPKDWNLKGAIFLSLDAQAELNY 167

Query: 116 ---------------------------------DRGEVIDGDLGKHFDLKLSNVIHLQER 175
                                            DRGEVIDGDLGKHFD++L      QE 
Sbjct: 168 RILDHLPYDSFVRKSVGYLFAIQHGAKKIFDADDRGEVIDGDLGKHFDVELVGEDARQEP 227

Query: 176 ILQFDFENPNKTVVNPYIHSGQRS-------------------------------EGISN 235
           ILQ+  ENPN+TVVNPYIH GQRS                               +GISN
Sbjct: 228 ILQYSHENPNRTVVNPYIHFGQRSVWPRGLPLENVGEINHEEYYTEVFGGKQFIQQGISN 287

Query: 236 GLPDVDSVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------- 295
           GLPDVDSV+Y TRKT  E  +IRFDEH+PKVALP G+M                      
Sbjct: 288 GLPDVDSVYYSTRKTTFEPFDIRFDEHSPKVALPQGMMVPVNSFNTLYHSSAFWGLMLPV 347

Query: 296 -----------GLWG-----------------------TEAFMGSRR-----FCSGLVKF 355
                      G WG                        EA+  S           L+KF
Sbjct: 348 SVSSMASDVIRGYWGQRLLWELGGYVAVYPPTVHRYDRVEAYPFSDEKDLHINVGRLIKF 407

Query: 356 LTSWRSNKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVS- 415
           L +WRSNK  FFE  L+LS  MAE+GFW + DVK + AWLQDL+ VG ++   MS+ +  
Sbjct: 408 LLAWRSNKHRFFETILDLSFVMAEQGFWTELDVKFTAAWLQDLLMVGYQQPRLMSLELDR 467

Query: 416 --VSIMGG---VFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNVVDDLSMAGQIGS 475
              +I  G    FVP+KLP    GVEE  TV+ EIG L++WRK FGNVV  +   G +  
Sbjct: 468 PRATIGHGDRKEFVPRKLPSVHLGVEEIGTVSSEIGNLIKWRKNFGNVVLIMFCNGPVER 527

Query: 476 GG---KLLWS--------------------------------------SYTKGFLFLQDN 531
                +LL+                                       S   GF+F++D+
Sbjct: 528 TALEWRLLYGRIFKTVVILSSRKNSDLYVQEAKLDHIYKRLPKIFDRYSSADGFVFVEDD 587

BLAST of Carg27318-RA vs. ExPASy TrEMBL
Match: A0A6J1GP46 (probable glycosyltransferase STELLO2 OS=Cucurbita moschata OX=3662 GN=LOC111456229 PE=4 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 1.2e-196
Identity = 412/718 (57.38%), Postives = 435/718 (60.58%), Query Frame = 0

Query: 8   QGAPDSAAFLMLATLSPKPLTSFICP------ISTLITCSSHCRQVFDLRPLLASDRWIV 67
           QGAPDSAAFL   +  PKP   F  P      +  ++  SS            +SDRWIV
Sbjct: 56  QGAPDSAAFLCFNS-RPKPPDLFHLPNINFDSVHPIVDKSSTYAS-------FSSDRWIV 115

Query: 68  VSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------------- 127
           VSVS  S+P    +   K R WQVLAVGNSR    WSL+ + F                 
Sbjct: 116 VSVS--SYPSDSLRKLAKTRGWQVLAVGNSRTPSDWSLKGVIFLSLEQQSSLGFRVVDFL 175

Query: 128 ---------------------------DRGEVIDGDLGKHFDLKLSNVIHLQERILQFDF 187
                                      DRGEVIDGDLGKHFDLKLSNV  LQERILQFDF
Sbjct: 176 PYDSYARKTVGYLFAIQHGAKMIFDADDRGEVIDGDLGKHFDLKLSNVDTLQERILQFDF 235

Query: 188 ENPNKTVVNPYIHSGQRS-------------------------------EGISNGLPDVD 247
           ENPNKTVVNPYIH GQRS                               +GISNGLPDVD
Sbjct: 236 ENPNKTVVNPYIHFGQRSVWPRGLPLENVGDVVYEEHYSQVFGGLQFIQQGISNGLPDVD 295

Query: 248 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------------- 307
           SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM                            
Sbjct: 296 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVMVPLNSFNTLFHTSALWALMLPASVSTMA 355

Query: 308 -----GLWGTEAFMGSRRF----------------------------CSGLVKFLTSWRS 367
                G W          F                               LVKFLTSWRS
Sbjct: 356 SDILRGYWAQRLLWEVGGFVVVYPPTMFRHDDIEGYPFSEEKDLHVNVGRLVKFLTSWRS 415

Query: 368 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG---------EEEEEAMSVS 427
           NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG         E  ++     
Sbjct: 416 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGYIQPRLKGFEMNKQRRGRR 475

Query: 428 VSVSIMGG-VFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNV-----VDDLSMAGQ 487
            +V +  G  FVPQKLPGF  GVEESETVNFEIGKLVRWRKKFGNV     V++  +  +
Sbjct: 476 SNVGVDNGRSFVPQKLPGFHLGVEESETVNFEIGKLVRWRKKFGNVVMILFVENGGVVDR 535

Query: 488 IGSGGKLLW--------------------------------------SSYTKGFLFLQDN 531
                +LL+                                      S   +GFLFLQDN
Sbjct: 536 TAMKWRLLYGRIFKTVVVVAEHGRADLGVEEASLEFIYKYLPTVFERSPNAEGFLFLQDN 595

BLAST of Carg27318-RA vs. ExPASy TrEMBL
Match: A0A6J1JN23 (probable glycosyltransferase STELLO2 OS=Cucurbita maxima OX=3661 GN=LOC111488378 PE=4 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 5.5e-194
Identity = 407/717 (56.76%), Postives = 429/717 (59.83%), Query Frame = 0

Query: 8   QGAPDSAAFLMLATLSPKPLTSFICP------ISTLITCSSHCRQVFDLRPLLASDRWIV 67
           QGA DSAAFL   +  PKP   F  P      +  ++  SS            +SDRWIV
Sbjct: 56  QGARDSAAFLCFNS-RPKPPELFHLPKINFDSVHPIVDKSSSYAS-------FSSDRWIV 115

Query: 68  VSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------------- 127
           VSVS  S+P    +   K R WQVLAVGNSR    WSL+ + F                 
Sbjct: 116 VSVS--SYPSDSLRKLAKTRGWQVLAVGNSRTPSDWSLKGVIFLSLEQQSSLGFRVVDFL 175

Query: 128 ---------------------------DRGEVIDGDLGKHFDLKLSNVIHLQERILQFDF 187
                                      DR EVIDGDLGKHFDLKLSNV  LQERILQFDF
Sbjct: 176 PYDSYARKTVGYLFAIQHGAKMIFDADDRCEVIDGDLGKHFDLKLSNVDTLQERILQFDF 235

Query: 188 ENPNKTVVNPYIHSGQRS-------------------------------EGISNGLPDVD 247
           ENPNKTVVNPYIH GQRS                               +GISNGLPDVD
Sbjct: 236 ENPNKTVVNPYIHFGQRSVWPRGLPLENVGDVVYEEHYSQVFGGLQFIQQGISNGLPDVD 295

Query: 248 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------------- 307
           SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM                            
Sbjct: 296 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVMVPLNSFNTLFHTSALWALMLPASVSTMA 355

Query: 308 -----GLWGTEAFMGSRRF----------------------------CSGLVKFLTSWRS 367
                G W          F                               L+KFLTSWRS
Sbjct: 356 SDILRGYWAQRLLWELGGFVVVYPPTMFRHDDIEGYPFSEEKDLHVNVGRLIKFLTSWRS 415

Query: 368 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG---------EEEEEAMSVS 427
           NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVG         E  ++    S
Sbjct: 416 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGYIQPRLKGFEMNKQRRRRS 475

Query: 428 VSVSIMGGVFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNV-----VDDLSMAGQI 487
                 G  FVPQKLPGF  GVEESETVNFEIGKLVRWRKKFGNV     V++  +  + 
Sbjct: 476 NGGGDTGRSFVPQKLPGFHLGVEESETVNFEIGKLVRWRKKFGNVVMILFVENGGVVDRT 535

Query: 488 GSGGKLLWSSYTK--------------------------------------GFLFLQDNT 531
               +LL+    K                                      GFLFLQDNT
Sbjct: 536 AMKWRLLYGRIFKTVVVVAENGRADLGVEEASLEFIYKYLPTVFERFPNAEGFLFLQDNT 595

BLAST of Carg27318-RA vs. ExPASy TrEMBL
Match: A0A6J1DB44 (probable glycosyltransferase STELLO2 OS=Momordica charantia OX=3673 GN=LOC111018493 PE=4 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 6.1e-177
Identity = 371/708 (52.40%), Postives = 407/708 (57.49%), Query Frame = 0

Query: 8   QGAPDSAAFLMLATLSPKPLTSFICP------ISTLITCSSHCRQVFDLRPLLASDRWIV 67
           +GA DSA FL   +  PKP      P      I  L+  SS       +    +S RWIV
Sbjct: 54  RGAADSATFLCFNS-RPKPAEPIHFPKINFDSIQPLVDKSS-------VYASFSSQRWIV 113

Query: 68  VSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------------- 127
           VSVS  S+P    +   K R WQVLAVGNSR    WSL+ + F                 
Sbjct: 114 VSVS--SYPSDSLRKLAKTRGWQVLAVGNSRTPSDWSLKGVIFLSLEQQSSLGFRVVDFL 173

Query: 128 ---------------------------DRGEVIDGDLGKHFDLKLSNVIHLQERILQFDF 187
                                      DRGEVIDGDLGKHFDLKL+NV  LQERILQFDF
Sbjct: 174 PYDSYARKTVGYLFAIQHGAKMIFDADDRGEVIDGDLGKHFDLKLTNVDTLQERILQFDF 233

Query: 188 ENPNKTVVNPYIHSGQRS-------------------------------EGISNGLPDVD 247
            NPNKTVVNPYIH GQRS                               +GISNGLPDVD
Sbjct: 234 TNPNKTVVNPYIHFGQRSVWPRGLPLENVGDVVYEEHYTQVFGGMQFIQQGISNGLPDVD 293

Query: 248 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------------- 307
           SVFYFTRKT SE  +IRFDEHAPKVALP G+M                            
Sbjct: 294 SVFYFTRKTGSETFDIRFDEHAPKVALPQGLMVPLNSFNTLFHISALWALMLPVSVSTMA 353

Query: 308 -----GLWGTEAFMGSRRF----------------------------CSGLVKFLTSWRS 367
                G W          F                               LVKFL SWRS
Sbjct: 354 SDVLRGYWAQRLLWEIGGFVVVYPPTMFRHDNIEAYPFAEEKDLHVNVGRLVKFLASWRS 413

Query: 368 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVS-VSIMGG 427
           NKATFFEKAL+LSHSMAEEGFW++NDVK++ AWL+DLVSVG  E       V        
Sbjct: 414 NKATFFEKALDLSHSMAEEGFWRENDVKMTAAWLEDLVSVGYIEPRVKGFEVKRERRRRR 473

Query: 428 VFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNVVDDLSMAGQIGSGGKLLWS---- 487
            FVPQKLPGF   VEE+ETVNFEIGKL+RWRKKFGNVV  L + G       + W     
Sbjct: 474 SFVPQKLPGFHLAVEETETVNFEIGKLIRWRKKFGNVVMVLFVDGGAVDRTAMKWRLLYG 533

Query: 488 --------------------------------------SYTKGFLFLQDNTILNYWNLLQ 531
                                                 S  +GFLFLQDNTILNYWNLLQ
Sbjct: 534 RIFKTVVVVAEHGRADLGVEEASLEFIYKYLPTVFERFSNAEGFLFLQDNTILNYWNLLQ 593

BLAST of Carg27318-RA vs. ExPASy TrEMBL
Match: A0A0A0K894 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G426450 PE=4 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 7.4e-175
Identity = 375/712 (52.67%), Postives = 409/712 (57.44%), Query Frame = 0

Query: 8   QGAPDSAAFLMLATLSPKPLTSFICP------ISTLITCSSHCRQVFDLRPLLASDRWIV 67
           +GAPDSAAFL   +  PKP      P      I  L+  SS            +SDRWIV
Sbjct: 56  RGAPDSAAFLCFNS-RPKPSQLTHLPIINFDSIHPLVDKSSSYAS-------FSSDRWIV 115

Query: 68  VSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------------- 127
           VSVS  S+P    +   K R WQVLAVGNSR    WSL+ + +                 
Sbjct: 116 VSVS--SYPSDSLRKLAKTRGWQVLAVGNSRTPSDWSLKGVIYLSLEEQSSLGFRVVDFL 175

Query: 128 ---------------------------DRGEVIDGDLGKHFDLKLSNVIHLQERILQFDF 187
                                      DRGEVIDGDLGKHFDLKLSNV  LQERIL+FDF
Sbjct: 176 SYDSYARKTVGYLFAIQHGAKMIFDADDRGEVIDGDLGKHFDLKLSNVDTLQERILEFDF 235

Query: 188 ENPNKTVVNPYIHSGQRS-------------------------------EGISNGLPDVD 247
           ENPNKTVVNPYIH GQRS                               +GISNGLPDVD
Sbjct: 236 ENPNKTVVNPYIHFGQRSVWPRGLPLENVGDVLYEEHYSQVFGGMQFIQQGISNGLPDVD 295

Query: 248 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------------- 307
           SVFYFTRKT S+A +IRFD+HAPKVA+PHGVM                            
Sbjct: 296 SVFYFTRKTSSQAFDIRFDDHAPKVAIPHGVMVPLNSFNTLFHNSALWALMLPVSVSTMA 355

Query: 308 -----GLWGTEAFMGSRRFCS----------------------------GLVKFLTSWRS 367
                G W          F +                             LVKFL+SW S
Sbjct: 356 CDILRGYWAQRLLWELGGFVAVYPPTMFRYDDIEGYPFSEEKDLHVNVGRLVKFLSSWTS 415

Query: 368 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVSVSIM--- 427
           NKATFFEK +ELS+SM EEGFWK+NDVKL  AWLQDL+SVG  +       +        
Sbjct: 416 NKATFFEKVMELSNSMEEEGFWKENDVKLIGAWLQDLISVGYIQPRMKGFEMKKQRKRRI 475

Query: 428 --GGVFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNVVDDLSMAG----QIGSGGK 487
             G  FVP+KLPGF  GVEESETVNFEIGKL+RWRKKFGNVV  L +      +     K
Sbjct: 476 GDGRSFVPKKLPGFHLGVEESETVNFEIGKLIRWRKKFGNVVMVLFVENGDVERTAMKWK 535

Query: 488 LLWSSYTK--------------------------------------GFLFLQDNTILNYW 531
           LL+    K                                      GFLFLQDNTILNYW
Sbjct: 536 LLYGRIFKTVVVVAEHGREDLGVEEASLEFIYKYLPMVFERFPNAEGFLFLQDNTILNYW 595

BLAST of Carg27318-RA vs. ExPASy TrEMBL
Match: A0A1S3CE29 (uncharacterized protein LOC103499945 OS=Cucumis melo OX=3656 GN=LOC103499945 PE=4 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.8e-173
Identity = 374/712 (52.53%), Postives = 407/712 (57.16%), Query Frame = 0

Query: 8   QGAPDSAAFLMLATLSPKPLTSFICP------ISTLITCSSHCRQVFDLRPLLASDRWIV 67
           +GAPDSAAFL   +  PKP      P      I  L+  SS            +SDRWIV
Sbjct: 56  RGAPDSAAFLCFNS-RPKPSQLTHLPKINFDSIHPLVDKSSSYAS-------FSSDRWIV 115

Query: 68  VSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------------- 127
           VSVS  S+P    +   K R WQVLAVGNSR    WSL+ + +                 
Sbjct: 116 VSVS--SYPSDSLRKLAKTRGWQVLAVGNSRTPSDWSLKGVIYLSLEEQSSLGFRVVDFL 175

Query: 128 ---------------------------DRGEVIDGDLGKHFDLKLSNVIHLQERILQFDF 187
                                      DRGEVIDG+LGKHFDLKLSNV  LQERIL+FDF
Sbjct: 176 SYDSYARKTVGYLFAIQHGAKMIFDADDRGEVIDGNLGKHFDLKLSNVDTLQERILEFDF 235

Query: 188 ENPNKTVVNPYIHSGQRS-------------------------------EGISNGLPDVD 247
           ENPNKTVVNPYIH GQRS                               +GISNGLPDVD
Sbjct: 236 ENPNKTVVNPYIHFGQRSVWPRGLPLENVGDVLYEEHYNQIFGGMQFIQQGISNGLPDVD 295

Query: 248 SVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------------- 307
           SVFYFTRKT S+A +IRFD+HAPKVA+PHGVM                            
Sbjct: 296 SVFYFTRKTSSQAFDIRFDDHAPKVAIPHGVMVPLNSFNTLFHNSALWALMLPVSVSTMA 355

Query: 308 -----GLWGTEAFMGSRRF----------------------------CSGLVKFLTSWRS 367
                G W          F                               LVKFL+SW S
Sbjct: 356 CDILRGYWAQRLLWELGGFVVVYPPTMFRYDDIEGYPFSEEKDLHVNVGRLVKFLSSWTS 415

Query: 368 NKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVSVSIM--- 427
           NKATFFEK +ELS+SM EEGFWK+NDVKL  AWLQDLVSVG  +       +        
Sbjct: 416 NKATFFEKVMELSNSMGEEGFWKENDVKLIGAWLQDLVSVGYIQPRMKGFEMKKQRKRRI 475

Query: 428 --GGVFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNVVDDLSMAG----QIGSGGK 487
             G  FVP+KLPGF  GVEESETVNFEIGKL+RWRK+FGNVV  L +      +     K
Sbjct: 476 GDGRSFVPKKLPGFHLGVEESETVNFEIGKLIRWRKRFGNVVMVLFVENGDVERTAMKWK 535

Query: 488 LLWSSYTK--------------------------------------GFLFLQDNTILNYW 531
           LL+    K                                      GFLFLQDNTILNYW
Sbjct: 536 LLYGRIFKTVVVVAEHGREDLGVEEASLEFIYKYLPMVFEKFPNAEGFLFLQDNTILNYW 595

BLAST of Carg27318-RA vs. TAIR 10
Match: AT2G41770.1 (Protein of unknown function (DUF288) )

HSP 1 Score: 439.9 bits (1130), Expect = 2.9e-123
Identity = 273/661 (41.30%), Postives = 347/661 (52.50%), Query Frame = 0

Query: 56  SDRWIVVSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------- 115
           +++WIVVSV+   +P    K+ VK+R WQVLA+GNS     WSL+   F           
Sbjct: 115 TEKWIVVSVT--KYPTEELKSLVKIRGWQVLAIGNSATPKDWSLKGSIFLSLDAQAELGY 174

Query: 116 ---------------------------------DRGEVIDGDLGKHFDLKLSNVIHLQER 175
                                            DRGEVIDGDLGKHFD++L  +   QE 
Sbjct: 175 RVLDHLPYDSFVRKSVGYLFAIQHGAKKIYDADDRGEVIDGDLGKHFDVELVGLDSKQEP 234

Query: 176 ILQFDFENPNKTVVNPYIHSGQRS-------------------------------EGISN 235
           ILQ+  ENPN+TVVNPYIH GQRS                               +GISN
Sbjct: 235 ILQYSHENPNRTVVNPYIHFGQRSVWPRGLPLENVGEINHEEYYTEVFGGKQFIQQGISN 294

Query: 236 GLPDVDSVFYFTRKTRSEALNIRFDEHAPKVALPHGVM-------GLWGTEAFMG----- 295
           GLPDVDSVFYFTRKT  EA +IRFDEH+PKVALP GVM        L+ + AF G     
Sbjct: 295 GLPDVDSVFYFTRKTTLEAFDIRFDEHSPKVALPQGVMVPVNSFNTLYHSSAFWGLMLPV 354

Query: 296 --------------------------------SRRF-----------------CSGLVKF 355
                                           + RF                    L+KF
Sbjct: 355 SVSSMASDVLRGYWGQRLLWELGGYVAVYPPTAHRFDRIEAYPFVEEKDLHVNVGRLIKF 414

Query: 356 LTSWRSNKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVS- 415
           L +WRS K +FFE  L+LS +MAEEGFW + D+K + AWLQDL++VG ++   MS+ +  
Sbjct: 415 LLAWRSEKHSFFETVLDLSFAMAEEGFWTEQDLKFTAAWLQDLIAVGYQQPRLMSLELDR 474

Query: 416 --VSIMGG---VFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNVVDDLSMAGQIGS 475
              SI  G    FVP+KLP    GVEE+ TV+ EIG L+RWRK FGNVV  +   G +  
Sbjct: 475 PRASIGHGDRKEFVPRKLPSVHLGVEETGTVSTEIGNLIRWRKNFGNVVLVMFCNGPVER 534

Query: 476 GG---KLLWS--------------------------------------SYTKGFLFLQDN 531
                +LL+                                       S  +GFLF++D+
Sbjct: 535 TALEWRLLYGRIFKTVVILSSQKNSDLYVEEAKLDHIYKHLPKIFDRYSSAEGFLFVEDD 594

BLAST of Carg27318-RA vs. TAIR 10
Match: AT3G57420.1 (Protein of unknown function (DUF288) )

HSP 1 Score: 430.6 bits (1106), Expect = 1.8e-120
Identity = 271/662 (40.94%), Postives = 341/662 (51.51%), Query Frame = 0

Query: 56  SDRWIVVSVSVLSFPHSPAKACVKLR-WQVLAVGNSRRSIGWSLRELYFY---------- 115
           +++WIVVSV+    P    K  VK++ WQVLA+GNS     W+L+   F           
Sbjct: 108 TEKWIVVSVT--KHPTEELKGLVKIKGWQVLAIGNSLTPKDWNLKGAIFLSLDAQAELNY 167

Query: 116 ---------------------------------DRGEVIDGDLGKHFDLKLSNVIHLQER 175
                                            DRGEVIDGDLGKHFD++L      QE 
Sbjct: 168 RILDHLPYDSFVRKSVGYLFAIQHGAKKIFDADDRGEVIDGDLGKHFDVELVGEDARQEP 227

Query: 176 ILQFDFENPNKTVVNPYIHSGQRS-------------------------------EGISN 235
           ILQ+  ENPN+TVVNPYIH GQRS                               +GISN
Sbjct: 228 ILQYSHENPNRTVVNPYIHFGQRSVWPRGLPLENVGEINHEEYYTEVFGGKQFIQQGISN 287

Query: 236 GLPDVDSVFYFTRKTRSEALNIRFDEHAPKVALPHGVM---------------------- 295
           GLPDVDSV+Y TRKT  E  +IRFDEH+PKVALP G+M                      
Sbjct: 288 GLPDVDSVYYSTRKTTFEPFDIRFDEHSPKVALPQGMMVPVNSFNTLYHSSAFWGLMLPV 347

Query: 296 -----------GLWG-----------------------TEAFMGSRR-----FCSGLVKF 355
                      G WG                        EA+  S           L+KF
Sbjct: 348 SVSSMASDVIRGYWGQRLLWELGGYVAVYPPTVHRYDRVEAYPFSDEKDLHINVGRLIKF 407

Query: 356 LTSWRSNKATFFEKALELSHSMAEEGFWKDNDVKLSVAWLQDLVSVGEEEEEAMSVSVS- 415
           L +WRSNK  FFE  L+LS  MAE+GFW + DVK + AWLQDL+ VG ++   MS+ +  
Sbjct: 408 LLAWRSNKHRFFETILDLSFVMAEQGFWTELDVKFTAAWLQDLLMVGYQQPRLMSLELDR 467

Query: 416 --VSIMGG---VFVPQKLPGFSSGVEESETVNFEIGKLVRWRKKFGNVVDDLSMAGQIGS 475
              +I  G    FVP+KLP    GVEE  TV+ EIG L++WRK FGNVV  +   G +  
Sbjct: 468 PRATIGHGDRKEFVPRKLPSVHLGVEEIGTVSSEIGNLIKWRKNFGNVVLIMFCNGPVER 527

Query: 476 GG---KLLWS--------------------------------------SYTKGFLFLQDN 531
                +LL+                                       S   GF+F++D+
Sbjct: 528 TALEWRLLYGRIFKTVVILSSRKNSDLYVQEAKLDHIYKRLPKIFDRYSSADGFVFVEDD 587

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014260.10.0e+00100.00putative glycosyltransferase STELLO1, partial [Cucurbita argyrosperma subsp. arg... [more]
KAG7014258.16.2e-20059.01putative glycosyltransferase STELLO1, partial [Cucurbita argyrosperma subsp. arg... [more]
XP_022953811.12.4e-19657.38probable glycosyltransferase STELLO2 [Cucurbita moschata][more]
KAG6575702.17.1e-19657.06putative glycosyltransferase STELLO1, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022991857.11.1e-19356.76probable glycosyltransferase STELLO2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O229434.1e-12241.30Probable glycosyltransferase STELLO1 OS=Arabidopsis thaliana OX=3702 GN=STL1 PE=... [more]
Q9SCN02.5e-11940.94Probable glycosyltransferase STELLO2 OS=Arabidopsis thaliana OX=3702 GN=STL2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1GP461.2e-19657.38probable glycosyltransferase STELLO2 OS=Cucurbita moschata OX=3662 GN=LOC1114562... [more]
A0A6J1JN235.5e-19456.76probable glycosyltransferase STELLO2 OS=Cucurbita maxima OX=3661 GN=LOC111488378... [more]
A0A6J1DB446.1e-17752.40probable glycosyltransferase STELLO2 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A0A0K8947.4e-17552.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G426450 PE=4 SV=1[more]
A0A1S3CE291.8e-17352.53uncharacterized protein LOC103499945 OS=Cucumis melo OX=3656 GN=LOC103499945 PE=... [more]
Match NameE-valueIdentityDescription
AT2G41770.12.9e-12341.30Protein of unknown function (DUF288) [more]
AT3G57420.11.8e-12040.94Protein of unknown function (DUF288) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR31362GLYCOSYLTRANSFERASE STELLO1-RELATEDcoord: 105..155
NoneNo IPR availablePANTHERPTHR31362:SF11GLYCOSYLTRANSFERASE STELLO2-RELATEDcoord: 156..198
NoneNo IPR availablePANTHERPTHR31362GLYCOSYLTRANSFERASE STELLO1-RELATEDcoord: 54..102
NoneNo IPR availablePANTHERPTHR31362GLYCOSYLTRANSFERASE STELLO1-RELATEDcoord: 342..530
coord: 216..331
NoneNo IPR availablePANTHERPTHR31362:SF11GLYCOSYLTRANSFERASE STELLO2-RELATEDcoord: 54..102
NoneNo IPR availablePANTHERPTHR31362:SF11GLYCOSYLTRANSFERASE STELLO2-RELATEDcoord: 342..530
coord: 216..331
NoneNo IPR availablePANTHERPTHR31362:SF11GLYCOSYLTRANSFERASE STELLO2-RELATEDcoord: 105..155
NoneNo IPR availablePANTHERPTHR31362GLYCOSYLTRANSFERASE STELLO1-RELATEDcoord: 156..198

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg27318Carg27318gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27318-RA:1Carg27318-RA:1exon
Carg27318-RA:2Carg27318-RA:2exon
Carg27318-RA:3Carg27318-RA:3exon
Carg27318-RA:4Carg27318-RA:4exon
Carg27318-RA:5Carg27318-RA:5exon
Carg27318-RA:6Carg27318-RA:6exon
Carg27318-RA:7Carg27318-RA:7exon
Carg27318-RA:8Carg27318-RA:8exon
Carg27318-RA:9Carg27318-RA:9exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27318-RA:cdsCarg27318-RA:cdsCDS
Carg27318-RA:cdsCarg27318-RA:cds_2CDS
Carg27318-RA:cdsCarg27318-RA:cds_3CDS
Carg27318-RA:cdsCarg27318-RA:cds_4CDS
Carg27318-RA:cdsCarg27318-RA:cds_5CDS
Carg27318-RA:cdsCarg27318-RA:cds_6CDS
Carg27318-RA:cdsCarg27318-RA:cds_7CDS
Carg27318-RA:cdsCarg27318-RA:cds_8CDS
Carg27318-RA:cdsCarg27318-RA:cds_9CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27318-RA:three_prime_utrCarg27318-RA:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg27318-RACarg27318-RA-proteinpolypeptide