Carg26424-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg26424-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description6-phosphogluconate dehydrogenase family protein
LocationCarg_Chr04: 2770287 .. 2772284 (-)
Sequence length1196
RNA-Seq ExpressionCarg26424-RA
SyntenyCarg26424-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATATTTGGACTAATATCTCTAAACTTAGCCATTATTATAGATTTGACGAACAAAATAAAGAATACGTACTTTATTTAGAATATATTCGTGTGGATCTAAAACATTCCTCAATTATCATCCGGACTCTAAACAAACCAAGATCCATGGCGGTGTCTGTTGATCATCTGTAAGTTGCTTTTGATAAATCCTTATTCCACCATGGAAACCCCTTACCCAATCCCCATCTCTCCTTCTAAAACTCGCGTTGGATGGATCGGTATAGGTGTGATGGGCGCCGCCATGGCCACTCGTGTCCTCGCCGCCGGATACTCCCTCACAATCTACGCTCGTTCCCCTTCCAAGGCTCTTGATATACAGTCCAAAGGCGCTTCCCTCGTCAATTCCCCTTCCGAATTAGCTCAGACTTGCGACGTTGTTTTCACAATGGTGGGAAACCCATCAGATGTTCGACAAAATGTCTTAGAGAAGAATGGGCTCCTACAAGGACTAAATCCTGGTGGGGTCATCGTTGACACCACCACCAGCCACCCCGCTCTTGCCCGCGAGATCTTCGCTGCTGCTCGTGCAAAAGGTCTTGTTAATTTCCACTAAATTACTAGATTAAATCAACCCACCCTGTGTTAGAAATCACCAATTCCACCATGGTATAATGTTGTCAGCTTGCTTAATGTCTTGTACCAATATGACTTTCTTATTTATAAACTCATGATCTTAGCTAATGTCGGATTCTCTCCCAACGTACTGAACTAAGAAGTTCAAGCTTATTTACTCGTATCTCGGCAGGTTGCTGGGCTGTGGATGCGCCCGTGTCAGGAGGCGACATTGGTGCTAGAGATGGGCAGCTATCCATTTTTGGGGGTGGCGATGCAGAGGTAGTGAAGTGGTTATCACCTCTGTTTGAAGCAATGGGGAAAGTGACATACGTGGGTGAAGCAGGGTGTGGGCAGAGCTGCAAGATAGCGAACCAGATAGCGCTTGTGGGAAACATGATAGGGGTGAGTGAAGGGCTGGTGTTCGCTGAGCGGGCGGGGTTGGATGTGAAGCAGTTTGTGGAAGCTGTGAGGGGCGGGGCAGGAGGGTCGATGGCAATGGAGTTGTTCGGGGGGAGGATGATAGAGAGGGACTTCAGGCCTGGTGGGTATGCTGAGTATATGGTGAAGGATCTTGCCATGTGTGTGGACGTTGTGGAAGAAGGGGAAGATGTGGAGAGGATTGCCGTGCTGCCAGGGGCTGCATTGGCCATGCAATTGTTCTCTGCAATGGTGGCTAATGGAGATGGAAAGATTGGCTCTCAAGGGATAATCTCTGTTATTCAGAGGCTTAATGGTAAGTGAGTAAAGCTATGGGAAAGCCAACTCTCTGTCTGTTATGGTCATCATCTTTTATGACCATTTTAATGTATTAATAAAAACAGCTTTTAATGTTGTCACAATATTATTAAATTTCATCAATTATCTACTTCTATATAATTGTTGCAATTAATTACTTATATTCCATTAATGATGATATTTAGTTACATTAATTGTATAAGTTTAGAGATATGGTTTTGTAATTTTAGGTTAAATTACAAATTTGATGACAATATGATGAGATTCATTCATCGGGAAGGAATTGATGATCAAGAAAATGGAGAAATGACACACTCTACCTAGTACATGTGTCTCCGATAGCTGCATCGTGAATATATACGATACGACACGACAAACGTGATTGCATTCCTCCACAAATCCATCGTCATGACTGTTAGATCCCATATCGAATGAAACATTCTTTATAAAGATATGGAAATCTCTTCCTGTTTTAAAACCCTTAAAGGGAAGCGCGAAAGGGAAAACTCAAAGAAGGCAATATTTAGGAGCGGTGATCTTGGGCTGTTACAAATGGTATCAGAGCAAAGAGTTAGACATCGTGATGTGTCAGGCCAGCAAGGACGCTAGACCCAAAAGTAGAGTGCTACATCGATTGGAGCAGAGAACGAGTGTCCGCCACCGGCTAG

mRNA sequence

AAATATTTGGACTAATATCTCTAAACTTAGCCATTATTATAGATTTGACGAACAAAATAAAGAATACGTACTTTATTTAGAATATATTCGTGTGGATCTAAAACATTCCTCAATTATCATCCGGACTCTAAACAAACCAAGATCCATGGCGGTGTCTGTTGATCATCTGTAAGTTGCTTTTGATAAATCCTTATTCCACCATGGAAACCCCTTACCCAATCCCCATCTCTCCTTCTAAAACTCGCGTTGGATGGATCGGTATAGGTGTGATGGGCGCCGCCATGGCCACTCGTGTCCTCGCCGCCGGATACTCCCTCACAATCTACGCTCGTTCCCCTTCCAAGGCTCTTGATATACAGTCCAAAGGCGCTTCCCTCGTCAATTCCCCTTCCGAATTAGCTCAGACTTGCGACGTTGTTTTCACAATGGTGGGAAACCCATCAGATGTTCGACAAAATGTCTTAGAGAAGAATGGGCTCCTACAAGGACTAAATCCTGGTGGGGTCATCGTTGACACCACCACCAGCCACCCCGCTCTTGCCCGCGAGATCTTCGCTGCTGCTCGTGCAAAAGGTTGCTGGGCTGTGGATGCGCCCGTGTCAGGAGGCGACATTGGTGCTAGAGATGGGCAGCTATCCATTTTTGGGGGTGGCGATGCAGAGGTAGTGAAGTGGTTATCACCTCTGTTTGAAGCAATGGGGAAAGTGACATACGTGGGTGAAGCAGGGTGTGGGCAGAGCTGCAAGATAGCGAACCAGATAGCGCTTGTGGGAAACATGATAGGGGTGAGTGAAGGGCTGGTGTTCGCTGAGCGGGCGGGGTTGGATGTGAAGCAGTTTGTGGAAGCTGTGAGGGGCGGGGCAGGAGGGTCGATGGCAATGGAGTTGTTCGGGGGGAGGATGATAGAGAGGGACTTCAGGCCTGGTGGGTATGCTGAGTATATGGTGAAGGATCTTGCCATGTGTGTGGACGTTGTGGAAGAAGGGGAAGATGTGGAGAGGATTGCCGTGCTGCCAGGGGCTGCATTGGCCATGCAATTGTTCTCTGCAATGGTGGCTAATGGAGATGGAAAGATTGGCTCTCAAGGGATAATCTCTGTTATTCAGAGGCTTAATGGTAAGCCAGCAAGGACGCTAGACCCAAAAGTAGAGTGCTACATCGATTGGAGCAGAGAACGAGTGTCCGCCACCGGCTAG

Coding sequence (CDS)

ATGGAAACCCCTTACCCAATCCCCATCTCTCCTTCTAAAACTCGCGTTGGATGGATCGGTATAGGTGTGATGGGCGCCGCCATGGCCACTCGTGTCCTCGCCGCCGGATACTCCCTCACAATCTACGCTCGTTCCCCTTCCAAGGCTCTTGATATACAGTCCAAAGGCGCTTCCCTCGTCAATTCCCCTTCCGAATTAGCTCAGACTTGCGACGTTGTTTTCACAATGGTGGGAAACCCATCAGATGTTCGACAAAATGTCTTAGAGAAGAATGGGCTCCTACAAGGACTAAATCCTGGTGGGGTCATCGTTGACACCACCACCAGCCACCCCGCTCTTGCCCGCGAGATCTTCGCTGCTGCTCGTGCAAAAGGTTGCTGGGCTGTGGATGCGCCCGTGTCAGGAGGCGACATTGGTGCTAGAGATGGGCAGCTATCCATTTTTGGGGGTGGCGATGCAGAGGTAGTGAAGTGGTTATCACCTCTGTTTGAAGCAATGGGGAAAGTGACATACGTGGGTGAAGCAGGGTGTGGGCAGAGCTGCAAGATAGCGAACCAGATAGCGCTTGTGGGAAACATGATAGGGGTGAGTGAAGGGCTGGTGTTCGCTGAGCGGGCGGGGTTGGATGTGAAGCAGTTTGTGGAAGCTGTGAGGGGCGGGGCAGGAGGGTCGATGGCAATGGAGTTGTTCGGGGGGAGGATGATAGAGAGGGACTTCAGGCCTGGTGGGTATGCTGAGTATATGGTGAAGGATCTTGCCATGTGTGTGGACGTTGTGGAAGAAGGGGAAGATGTGGAGAGGATTGCCGTGCTGCCAGGGGCTGCATTGGCCATGCAATTGTTCTCTGCAATGGTGGCTAATGGAGATGGAAAGATTGGCTCTCAAGGGATAATCTCTGTTATTCAGAGGCTTAATGGTAAGCCAGCAAGGACGCTAGACCCAAAAGTAGAGTGCTACATCGATTGGAGCAGAGAACGAGTGTCCGCCACCGGCTAG

Protein sequence

METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLVNSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAAARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQSCKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFRPGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISVIQRLNGKPARTLDPKVECYIDWSRERVSATG
Homology
[Close]
BLAST of Carg26424-RA vs. NCBI nr
Match: KAG7031014.1 (putative 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 653.7 bits (1685), Expect = 8.7e-184
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV
Sbjct: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA
Sbjct: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR
Sbjct: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
           PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV
Sbjct: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300

Query: 301 IQRLNGKPARTLDPKVECYIDWSRERVSATG 332
           IQRLNGKPARTLDPKVECYIDWSRERVSATG
Sbjct: 301 IQRLNGKPARTLDPKVECYIDWSRERVSATG 331

[Close]
BLAST of Carg26424-RA vs. NCBI nr
Match: KAG6600355.1 (putative 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 600.9 bits (1548), Expect = 6.7e-168
Identity = 306/307 (99.67%), Postives = 307/307 (100.00%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALD+QSKGASLV
Sbjct: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDLQSKGASLV 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA
Sbjct: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR
Sbjct: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
           PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV
Sbjct: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300

Query: 301 IQRLNGK 308
           IQRLNGK
Sbjct: 301 IQRLNGK 307

[Close]
BLAST of Carg26424-RA vs. NCBI nr
Match: XP_022942974.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucurbita moschata])

HSP 1 Score: 591.3 bits (1523), Expect = 5.3e-165
Identity = 299/307 (97.39%), Postives = 306/307 (99.67%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYPIPISPSKTRVGWIGIGVMGAAMATR+LAAGYSLTIYARSPSKALD+QSKGASLV
Sbjct: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRLLAAGYSLTIYARSPSKALDLQSKGASLV 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NSPSELAQTCDVVFTMVGNPSDVRQNVL+KNGLLQGLNPGGVIVDTT+SHPALAREIFAA
Sbjct: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLQKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDG+LSIFGGGDAEVVKWLSPLFEA+GKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLSIFGGGDAEVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKIANQI LVGNMIGVSEGLVFAE+AGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR
Sbjct: 181 CKIANQITLVGNMIGVSEGLVFAEQAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
           PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV
Sbjct: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300

Query: 301 IQRLNGK 308
           IQRLNGK
Sbjct: 301 IQRLNGK 307

[Close]
BLAST of Carg26424-RA vs. NCBI nr
Match: XP_022982050.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucurbita maxima])

HSP 1 Score: 584.3 bits (1505), Expect = 6.4e-163
Identity = 296/307 (96.42%), Postives = 303/307 (98.70%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYPIPISPSKTRVGWIGIGVMGAAMATR+LAAGYSLTIYARSPSKALD+QSKGASLV
Sbjct: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRLLAAGYSLTIYARSPSKALDLQSKGASLV 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NSPSELA TCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTT+SHPALAREI AA
Sbjct: 61  NSPSELAHTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREISAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDG+LSIFGGGDAEVVKWLSPLFEA+GKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLSIFGGGDAEVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKIANQI LVGNMIGVSEGLVFAERAGLDVKQFVEAVR GAGGSMAMELFGGRM++RDFR
Sbjct: 181 CKIANQITLVGNMIGVSEGLVFAERAGLDVKQFVEAVRDGAGGSMAMELFGGRMVKRDFR 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
           PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV
Sbjct: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300

Query: 301 IQRLNGK 308
           IQRLNGK
Sbjct: 301 IQRLNGK 307

[Close]
BLAST of Carg26424-RA vs. NCBI nr
Match: XP_023533851.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 549.7 bits (1415), Expect = 1.8e-152
Identity = 278/331 (83.99%), Postives = 306/331 (92.45%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYPIPISP+KTR+GWIGIGVMGAAMATR+LAAGYS+ IYARSPSKALD+QSKGASLV
Sbjct: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NS SELAQ  D+VFTMVG+PSDVRQNVLEKNGLLQGLNPGGVIVDTT+SHPALAREIFAA
Sbjct: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDG+L+IFGGGDA VVKWLSPLFEA+GKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKIANQIA+ GN++G+SEGLVFAERAGLDVKQFVEAVRGGA GSMAMELFG RMIERDFR
Sbjct: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
           PGG+AEYMVKDL M VDVVEEGEDVER+ VLPGAALA QLFSAMVANGDGK G+QG+ISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 IQRLNGK----PARTLDPKVECYIDWSRERV 328
           +QRLNGK     ++TL   + C++D  + ++
Sbjct: 301 VQRLNGKIYSCGSKTL---LNCHLDSKQTKI 328

[Close]
BLAST of Carg26424-RA vs. ExPASy Swiss-Prot
Match: Q949M8 (Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71180 PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 2.1e-103
Identity = 185/306 (60.46%), Postives = 235/306 (76.80%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYP  I PSKTR+GWIGIG+MG+AM + ++AAGYS+T+YAR   K  D+Q+KGA + 
Sbjct: 22  METPYPKLIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIA 81

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NSP ELA+  DVVFT+VGN +DVR  +L  +G+L GL PGGV VD T+S P LAREI A 
Sbjct: 82  NSPKELAEMSDVVFTIVGNFNDVRSLLLGDDGVLSGLTPGGVTVDMTSSKPGLAREIHAE 141

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+G L IF GGD+E+V+WLSP+ + +G VTY+GEAG GQS
Sbjct: 142 ARRRNCWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQS 201

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKI NQIA   N++G++EG+VFAE+AGLD  +++EAV+ GA GS  M LFG  +++RD+R
Sbjct: 202 CKIGNQIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYR 261

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
             G+AEYMVKDL M  +           A +PGAAL+ QLF+ MVANGDGK+G QG++SV
Sbjct: 262 ATGFAEYMVKDLGMAAE-----------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSV 316

Query: 301 IQRLNG 307
           I+RLNG
Sbjct: 322 IRRLNG 316

[Close]
BLAST of Carg26424-RA vs. ExPASy Swiss-Prot
Match: Q9C991 (Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71170 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.6e-98
Identity = 179/306 (58.50%), Postives = 226/306 (73.86%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           MET YP  I PSKTR+GWIGIG+MG+AM + +LAAGYS+T+YAR   K  D+Q+KG    
Sbjct: 1   METQYPKLIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTA 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NSP EL +  DVVFT+VGN +DVR  +L  +G+L GL PGGV VD T+S P LAREI+A 
Sbjct: 61  NSPKELGEMSDVVFTIVGNSNDVRSLLLGDDGVLSGLKPGGVTVDMTSSKPGLAREIYAE 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+G+L+IF GGD+E+V+WL+P+ + MG V ++G AG GQS
Sbjct: 121 ARRRDCWAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKI NQI +  NMIG++EG+VFAE+AGLD  +++EAV+ GA GS  M LFG  M  RD++
Sbjct: 181 CKIGNQICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYK 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
             G+AEYMVKDL M           E    +PG AL  QLF+ MVANGDGK+G QG++ V
Sbjct: 241 ATGFAEYMVKDLGMA---------AEAAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDV 297

Query: 301 IQRLNG 307
           I+RLNG
Sbjct: 301 IRRLNG 297

[Close]
BLAST of Carg26424-RA vs. ExPASy Swiss-Prot
Match: Q9SZE1 (Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g29120 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 4.2e-80
Identity = 149/307 (48.53%), Postives = 221/307 (71.99%), Query Frame = 0 

Query: 9   ISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLVNSPSELAQ 68
           I+PS T++GWIG GVMG +M   ++ AGY++T++ R+ SKA  +   GA++ +SP+ +A+
Sbjct: 33  ITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAE 92

Query: 69  TCDVVFTMVGNPSDVRQNVLE-KNGLLQGLNPGGVIVDTTTSHPALAREIFAAARAKGCW 128
             DVVFT+VG PSDVR  +L+ K+G L GL  GGV+VD TTS P+LA EI  AA  K C+
Sbjct: 93  QSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFKNCF 152

Query: 129 AVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQSCKIANQI 188
           ++DAPVSGGD+GA++G+LSIF GGD   VK L PLF  MGKV ++G +G GQ  K+ANQI
Sbjct: 153 SIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKLANQI 212

Query: 189 ALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFRPGGYAEY 248
            +   M+G+ EGL++A +AGLDVK+F+EA+  GA GS +++L+G R+++RDF PG Y  +
Sbjct: 213 TIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFYVNH 272

Query: 249 MVKDLAMCVDVVEEGEDVERIAV-LPGAALAMQLFSAMVANGDGKIGSQGIISVIQRLNG 308
            VKDL +C++      + +R+ + LPG ALA QL+ ++ A+G+G +G+Q ++  ++RLN 
Sbjct: 273 FVKDLGICLN------ECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLNN 332

Query: 309 KPARTLD 314
              ++ D
Sbjct: 333 VSVQSSD 333

[Close]
BLAST of Carg26424-RA vs. ExPASy Swiss-Prot
Match: O34948 (Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 GN=ykwC PE=3 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.0e-54
Identity = 111/287 (38.68%), Postives = 180/287 (62.72%), Query Frame = 0 

Query: 13  KTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLVNSPSELAQTCDV 72
           K  +G+IG+GVMG +MA+ +L  G+ + +Y R+  KA  I  KGA   ++  +L++  DV
Sbjct: 2   KKTIGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADV 61

Query: 73  VFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAAARAKGCWAVDAP 132
           + TMVG PSDV +     NG+++    G  ++D TTS P+LA++I  AA+ K  +A+DAP
Sbjct: 62  IITMVGYPSDVEEVYFGSNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAP 121

Query: 133 VSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGK-VTYVGEAGCGQSCKIANQIALVG 192
           VSGGDIGA++G L+I  GG+ E  +   P+F  MG+ + Y G AG GQ  K+ NQIA+  
Sbjct: 122 VSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIAA 181

Query: 193 NMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFRPGGYAEYMVKD 252
            MIGV+E + +A+++GL+ +  ++++  GA GS ++     RM++ +F PG Y ++ +KD
Sbjct: 182 GMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIKD 241

Query: 253 LAMCVDVVE-EGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGI 298
           + + ++  E  GE+      +PG +LA  L+  + A G+   G+Q I
Sbjct: 242 MGIALEEAELMGEE------MPGLSLAKSLYDKLAAQGEENSGTQSI 282

[Close]
BLAST of Carg26424-RA vs. ExPASy Swiss-Prot
Match: P0ABQ3 (2-hydroxy-3-oxopropionate reductase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=garR PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.7e-45
Identity = 104/291 (35.74%), Postives = 165/291 (56.70%), Query Frame = 0 

Query: 15  RVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLVNSPSELAQTCDVVF 74
           +VG+IG+G+MG  M+  +L AGYSL +  R+P    D+ + GA   ++   +A+ CDV+ 
Sbjct: 2   KVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVII 61

Query: 75  TMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAAARAKGCWAVDAPVS 134
           TM+ N   V++  L +NG+++G  PG V++D ++  P  +REI  A +AKG   +DAPVS
Sbjct: 62  TMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVS 121

Query: 135 GGDIGARDGQLSIFGGGDAEVVKWLSPLFEAM-GKVTYVGEAGCGQSCKIANQIALVGNM 194
           GG+  A DG LS+  GGD  +      L +AM G V + GE G G   K+ANQ+ +  N+
Sbjct: 122 GGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVALNI 181

Query: 195 IGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFRPGGYAEYMVKDLA 254
             +SE L  A +AG++     +A+RGG  GS  ++     +++R+F+PG   +  +KDLA
Sbjct: 182 AAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDLA 241

Query: 255 MCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISVIQRL 305
             +D    G      A LP  A  M++  A+ A+G G      +    ++L
Sbjct: 242 NALD-TSHGVG----AQLPLTAAVMEMMQALRADGLGTADHSALACYYEKL 287

[Close]
BLAST of Carg26424-RA vs. ExPASy TrEMBL
Match: A0A6J1FSW9 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111447849 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.6e-165
Identity = 299/307 (97.39%), Postives = 306/307 (99.67%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYPIPISPSKTRVGWIGIGVMGAAMATR+LAAGYSLTIYARSPSKALD+QSKGASLV
Sbjct: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRLLAAGYSLTIYARSPSKALDLQSKGASLV 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NSPSELAQTCDVVFTMVGNPSDVRQNVL+KNGLLQGLNPGGVIVDTT+SHPALAREIFAA
Sbjct: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLQKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDG+LSIFGGGDAEVVKWLSPLFEA+GKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLSIFGGGDAEVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKIANQI LVGNMIGVSEGLVFAE+AGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR
Sbjct: 181 CKIANQITLVGNMIGVSEGLVFAEQAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
           PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV
Sbjct: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300

Query: 301 IQRLNGK 308
           IQRLNGK
Sbjct: 301 IQRLNGK 307

[Close]
BLAST of Carg26424-RA vs. ExPASy TrEMBL
Match: A0A6J1J1I1 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111481012 PE=4 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.1e-163
Identity = 296/307 (96.42%), Postives = 303/307 (98.70%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYPIPISPSKTRVGWIGIGVMGAAMATR+LAAGYSLTIYARSPSKALD+QSKGASLV
Sbjct: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRLLAAGYSLTIYARSPSKALDLQSKGASLV 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NSPSELA TCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTT+SHPALAREI AA
Sbjct: 61  NSPSELAHTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREISAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDG+LSIFGGGDAEVVKWLSPLFEA+GKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLSIFGGGDAEVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKIANQI LVGNMIGVSEGLVFAERAGLDVKQFVEAVR GAGGSMAMELFGGRM++RDFR
Sbjct: 181 CKIANQITLVGNMIGVSEGLVFAERAGLDVKQFVEAVRDGAGGSMAMELFGGRMVKRDFR 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
           PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV
Sbjct: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300

Query: 301 IQRLNGK 308
           IQRLNGK
Sbjct: 301 IQRLNGK 307

[Close]
BLAST of Carg26424-RA vs. ExPASy TrEMBL
Match: A0A6J1FRQ4 (probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111447848 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.0e-150
Identity = 271/307 (88.27%), Postives = 292/307 (95.11%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYPIPISP+KTR+GWIGIGVMGAAMATR+LAAGYS+ IYARSPSKALD+QSKGASLV
Sbjct: 1   METPYPIPISPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NS SELAQ  D+VFTMVG+PSDVRQNVLEKNGLLQGLNPGGVIVDTT+SHPALAREIFAA
Sbjct: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDG+L+IFGGGDA VVKWLSPLFEA+GKV YVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVAYVGEAGCGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKIANQIA+ GN++G+ EGLVFAERAGLDVKQFVEA+RGGA GSMAMELFG RMIERDFR
Sbjct: 181 CKIANQIAIGGNLMGLGEGLVFAERAGLDVKQFVEALRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
           PGG+AEYMVKDL M VDVVEEGEDVER+ VLPGAALA QLFSAMVANGDGK G+QG+ISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERLVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 IQRLNGK 308
           +QRLNGK
Sbjct: 301 VQRLNGK 307

[Close]
BLAST of Carg26424-RA vs. ExPASy TrEMBL
Match: A0A6J1J121 (probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111480910 PE=4 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 5.2e-150
Identity = 272/307 (88.60%), Postives = 291/307 (94.79%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYPI I P+KTR+GWIGIGVMGAAMATR+LAAGYS+ IYARSPSKALD+QSKGASLV
Sbjct: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NS SELAQ  D+VFTMVG+PSDVRQNVLEKNGLLQGLNPGGVIVDTT+SHP LAREIFAA
Sbjct: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDG+L+IFGGGDA VVKWLSPLFEA+GKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKIANQIA+ GN++G+SEGLVFAERAGLDVKQFVEAVRGGA GSMAMELFG RMIERDFR
Sbjct: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
           PGG+AEYMVKDL M VDVVEEGEDVERI VLPGAALA QLFSAMVANGDGK G+QG+ISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 IQRLNGK 308
           +QRLNGK
Sbjct: 301 VQRLNGK 307

[Close]
BLAST of Carg26424-RA vs. ExPASy TrEMBL
Match: A0A6J1D789 (probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111017673 PE=4 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 8.8e-142
Identity = 256/307 (83.39%), Postives = 286/307 (93.16%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYPIP++P+KTR+GWIGIGVMGAAMATR+L+AGYS+ IYAR+PSKALD+QSKGA L+
Sbjct: 1   METPYPIPVTPTKTRIGWIGIGVMGAAMATRLLSAGYSVAIYARTPSKALDLQSKGARLL 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NS  E+A+T DVVFTMVG+PSDVRQNVLE NGLLQGLNPGGV+VDTT+SHPALAREIFAA
Sbjct: 61  NSAVEVARTSDVVFTMVGHPSDVRQNVLENNGLLQGLNPGGVVVDTTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           ARAK CWAVDAPVSGGDIGARDG+L+IF GGDA VVKWL+PLFE +GKVT+VGE+GCGQS
Sbjct: 121 ARAKDCWAVDAPVSGGDIGARDGKLAIFAGGDAGVVKWLAPLFEILGKVTHVGESGCGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKIANQI +  N++G+SEGLVFAERAGLD+KQFVEAVRGGA GSMAMELFG RMI RDFR
Sbjct: 181 CKIANQITIGANLMGLSEGLVFAERAGLDLKQFVEAVRGGAAGSMAMELFGERMIRRDFR 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
           PGG+AEYMVKDL M VDVVEEGED ERIAVLPGAALA QLFSAMVANGDGK G+QG+ISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGED-ERIAVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300

Query: 301 IQRLNGK 308
           I+RLNGK
Sbjct: 301 IERLNGK 306

[Close]
BLAST of Carg26424-RA vs. TAIR 10
Match: AT1G71180.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 377.1 bits (967), Expect = 1.5e-104
Identity = 185/306 (60.46%), Postives = 235/306 (76.80%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           METPYP  I PSKTR+GWIGIG+MG+AM + ++AAGYS+T+YAR   K  D+Q+KGA + 
Sbjct: 22  METPYPKLIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIA 81

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NSP ELA+  DVVFT+VGN +DVR  +L  +G+L GL PGGV VD T+S P LAREI A 
Sbjct: 82  NSPKELAEMSDVVFTIVGNFNDVRSLLLGDDGVLSGLTPGGVTVDMTSSKPGLAREIHAE 141

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+G L IF GGD+E+V+WLSP+ + +G VTY+GEAG GQS
Sbjct: 142 ARRRNCWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQS 201

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKI NQIA   N++G++EG+VFAE+AGLD  +++EAV+ GA GS  M LFG  +++RD+R
Sbjct: 202 CKIGNQIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYR 261

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
             G+AEYMVKDL M  +           A +PGAAL+ QLF+ MVANGDGK+G QG++SV
Sbjct: 262 ATGFAEYMVKDLGMAAE-----------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSV 316

Query: 301 IQRLNG 307
           I+RLNG
Sbjct: 322 IRRLNG 316

[Close]
BLAST of Carg26424-RA vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.8e-99
Identity = 179/306 (58.50%), Postives = 226/306 (73.86%), Query Frame = 0 

Query: 1   METPYPIPISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLV 60
           MET YP  I PSKTR+GWIGIG+MG+AM + +LAAGYS+T+YAR   K  D+Q+KG    
Sbjct: 1   METQYPKLIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTA 60

Query: 61  NSPSELAQTCDVVFTMVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAA 120
           NSP EL +  DVVFT+VGN +DVR  +L  +G+L GL PGGV VD T+S P LAREI+A 
Sbjct: 61  NSPKELGEMSDVVFTIVGNSNDVRSLLLGDDGVLSGLKPGGVTVDMTSSKPGLAREIYAE 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+G+L+IF GGD+E+V+WL+P+ + MG V ++G AG GQS
Sbjct: 121 ARRRDCWAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQS 180

Query: 181 CKIANQIALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFR 240
           CKI NQI +  NMIG++EG+VFAE+AGLD  +++EAV+ GA GS  M LFG  M  RD++
Sbjct: 181 CKIGNQICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYK 240

Query: 241 PGGYAEYMVKDLAMCVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISV 300
             G+AEYMVKDL M           E    +PG AL  QLF+ MVANGDGK+G QG++ V
Sbjct: 241 ATGFAEYMVKDLGMA---------AEAAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDV 297

Query: 301 IQRLNG 307
           I+RLNG
Sbjct: 301 IRRLNG 297

[Close]
BLAST of Carg26424-RA vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 299.7 bits (766), Expect = 3.0e-81
Identity = 149/307 (48.53%), Postives = 221/307 (71.99%), Query Frame = 0 

Query: 9   ISPSKTRVGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLVNSPSELAQ 68
           I+PS T++GWIG GVMG +M   ++ AGY++T++ R+ SKA  +   GA++ +SP+ +A+
Sbjct: 33  ITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAE 92

Query: 69  TCDVVFTMVGNPSDVRQNVLE-KNGLLQGLNPGGVIVDTTTSHPALAREIFAAARAKGCW 128
             DVVFT+VG PSDVR  +L+ K+G L GL  GGV+VD TTS P+LA EI  AA  K C+
Sbjct: 93  QSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFKNCF 152

Query: 129 AVDAPVSGGDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVTYVGEAGCGQSCKIANQI 188
           ++DAPVSGGD+GA++G+LSIF GGD   VK L PLF  MGKV ++G +G GQ  K+ANQI
Sbjct: 153 SIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKLANQI 212

Query: 189 ALVGNMIGVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFRPGGYAEY 248
            +   M+G+ EGL++A +AGLDVK+F+EA+  GA GS +++L+G R+++RDF PG Y  +
Sbjct: 213 TIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFYVNH 272

Query: 249 MVKDLAMCVDVVEEGEDVERIAV-LPGAALAMQLFSAMVANGDGKIGSQGIISVIQRLNG 308
            VKDL +C++      + +R+ + LPG ALA QL+ ++ A+G+G +G+Q ++  ++RLN 
Sbjct: 273 FVKDLGICLN------ECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLNN 332

Query: 309 KPARTLD 314
              ++ D
Sbjct: 333 VSVQSSD 333

[Close]
BLAST of Carg26424-RA vs. TAIR 10
Match: AT1G17650.1 (glyoxylate reductase 2 )

HSP 1 Score: 125.9 bits (315), Expect = 5.8e-29
Identity = 85/296 (28.72%), Postives = 143/296 (48.31%), Query Frame = 0 

Query: 16  VGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLVNSPSELAQTCDVVFT 75
           +G++G+G+MG+ MA  ++ AG  +T++ R+ SK   +   GA   +SP E+  TCD+ F 
Sbjct: 69  IGFLGMGIMGSPMAQNLIKAGCDVTVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTFA 128

Query: 76  MVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAAARAKGCWAVDAPVSG 135
           M+ +P         KNG + G++ G   VD +T   A +  I    +  G   ++APVSG
Sbjct: 129 MLADPESAIDVACGKNGAIFGISSGKGYVDVSTVDVASSILISKQIKDTGALFLEAPVSG 188

Query: 136 GDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVT-YVGEAGCGQSCKIANQIALVGNMI 195
               A DGQL     GD  + +  +P  + MGK   Y+GE G G + K+   + +   M 
Sbjct: 189 SKKPAEDGQLIFLTAGDKPLYEKAAPFLDIMGKSKFYLGEVGNGAAMKLVVNMIMGSMMA 248

Query: 196 GVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFRPGGYAEYMVKDLAM 255
             +EG++ +++ GLD    VE V  GA  +    L G  MI+  +      ++  KD+ +
Sbjct: 249 SFAEGILLSQKVGLDPNVLVEVVSQGAINAPMYSLKGPSMIKSVYPTAFPLKHQQKDMRL 308

Query: 256 CVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISVIQRLNGKPAR 311
            + + E           P AA A +L+    + G   +  +   +VI+ L    +R
Sbjct: 309 ALGLAESVSQ-----STPIAAAANELYKVAKSYG---LSDEDFSAVIEALKAAKSR 356

[Close]
BLAST of Carg26424-RA vs. TAIR 10
Match: AT3G25530.1 (glyoxylate reductase 1 )

HSP 1 Score: 120.9 bits (302), Expect = 1.9e-27
Identity = 81/288 (28.12%), Postives = 143/288 (49.65%), Query Frame = 0 

Query: 16  VGWIGIGVMGAAMATRVLAAGYSLTIYARSPSKALDIQSKGASLVNSPSELAQTCDVVFT 75
           VG++G+G+MG AM+  +L  G+ +T++ R+ SK  ++   GAS+  SP+E+ + C     
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 76  MVGNPSDVRQNVLEKNGLLQGLNPGGVIVDTTTSHPALAREIFAAARAKGCWAVDAPVSG 135
           M+ +P      V +K G+L+ +  G   +D +T     + +I  A   KG   V+ PVSG
Sbjct: 63  MLSDPCAALSVVFDKGGVLEQICEGKGYIDMSTVDAETSLKINEAITGKGGRFVEGPVSG 122

Query: 136 GDIGARDGQLSIFGGGDAEVVKWLSPLFEAMGKVT-YVGEAGCGQSCKIANQIALVGNMI 195
               A DGQL I   GD  + +   P F+ +GK + Y+G+ G G   K+   + +   M 
Sbjct: 123 SKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMKLIVNMIMGSMMN 182

Query: 196 GVSEGLVFAERAGLDVKQFVEAVRGGAGGSMAMELFGGRMIERDFRPGGYAEYMVKDLAM 255
             SEGLV A+++GL     ++ +  GA  +   +  G  M +  + P    ++  KD+ +
Sbjct: 183 AFSEGLVLADKSGLSSDTLLDILDLGAMTNPMFKGKGPSMNKSSYPPAFPLKHQQKDMRL 242

Query: 256 CVDVVEEGEDVERIAVLPGAALAMQLFSAMVANGDGKIGSQGIISVIQ 303
            + + +     E    +P AA A + F    + G G +    +I  ++
Sbjct: 243 ALALGD-----ENAVSMPVAAAANEAFKKARSLGLGDLDFSAVIEAVK 285

The following BLAST results are available for this feature:
BLAST of Carg26424-RA vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7031014.18.7e-184100.00putative 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucurbita arg... [more]
KAG6600355.16.7e-16899.67putative 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial, partial [Cucu... [more]
XP_022942974.15.3e-16597.39probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucurbita mos... [more]
XP_022982050.16.4e-16396.42probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucurbita max... [more]
XP_023533851.11.8e-15283.99probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial isoform X1 [Cu... [more]
BLAST of Carg26424-RA vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q949M82.1e-10360.46Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis... [more]
Q9C9912.6e-9858.50Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis... [more]
Q9SZE14.2e-8048.53Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis... [more]
O349481.0e-5438.68Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 ... [more]
P0ABQ35.7e-4535.742-hydroxy-3-oxopropionate reductase OS=Escherichia coli O6:H1 (strain CFT073 / A... [more]
BLAST of Carg26424-RA vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FSW92.6e-16597.39probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucurbita m... [more]
A0A6J1J1I13.1e-16396.42probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Cucurbita m... [more]
A0A6J1FRQ41.0e-15088.27probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Cucurbita m... [more]
A0A6J1J1215.2e-15088.60probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Cucurbita m... [more]
A0A6J1D7898.8e-14283.39probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Momordica c... [more]
BLAST of Carg26424-RA vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G71180.11.5e-10460.466-phosphogluconate dehydrogenase family protein [more]
AT1G71170.11.8e-9958.506-phosphogluconate dehydrogenase family protein [more]
AT4G29120.13.0e-8148.536-phosphogluconate dehydrogenase family protein [more]
AT1G17650.15.8e-2928.72glyoxylate reductase 2 [more]
AT3G25530.11.9e-2728.13glyoxylate reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0158153-hydroxyisobutyrate dehydrogenase-relatedPIRSFPIRSF000103HIBADHcoord: 10..309
e-value: 1.5E-63
score: 212.6
IPR0061156-phosphogluconate dehydrogenase, NADP-bindingPFAMPF03446NAD_binding_2coord: 15..173
e-value: 1.0E-44
score: 152.5
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 174..308
e-value: 6.5E-35
score: 122.2
IPR0291543-hydroxyisobutyrate dehydrogenase, NAD-binding domainPFAMPF14833NAD_binding_11coord: 176..299
e-value: 7.7E-26
score: 90.7
NoneNo IPR availableGENE3D3.40.50.720coord: 10..173
e-value: 7.7E-50
score: 170.9
NoneNo IPR availablePANTHERPTHR430603-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 1, MITOCHONDRIAL-RELATEDcoord: 1..307
NoneNo IPR availablePANTHERPTHR43060:SF133-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 2, MITOCHONDRIAL-RELATEDcoord: 1..307
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 14..172
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 174..307

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg26424Carg26424gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg26424-RA:3Carg26424-RA:3exon
Carg26424-RA:2Carg26424-RA:2exon
Carg26424-RA:1Carg26424-RA:1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg26424-RA:cdsCarg26424-RA:cds_3CDS
Carg26424-RA:cdsCarg26424-RA:cds_2CDS
Carg26424-RA:cdsCarg26424-RA:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg26424-RA:five_prime_utrCarg26424-RA:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg26424-RACarg26424-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051287 NAD binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0003676 nucleic acid binding