Carg24329-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24329-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	BolA protein
Location	Carg_Chr20: 10191900 .. 10194190 (-)
Sequence length	500
RNA-Seq Expression	Carg24329-RA
Synteny	Carg24329-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGCAGGAAGGCAATTGAAGAAAATGGGAGTCACAAAGGAGCAACTCGAATCTACTTTAACCTCCAAATTGAATCCTCAGCATCTGGTTTGCTTCTTCTTCACGCTCTTTCTCTACTTTTTTCTGATTATCGATCCTCGTGTTCTGTTCACCTGCGGTTTTCCTTTTCGTTCACTTAGTATTGAGCCAAAAATTGGCATCGTGTTCTTGCTAGTTTAGCTTTTCTAAGTCTTATTTCACTTCATTGCTTTGAATTGAGATAGTGGAACTGAGAAGAGATTTGGAAGTGTTATCTTCCGTTCCAATGGATGATTTTCTCTGCTTTTTGTTTTTACTTGGAGATCTTATGGTGTTTGAGTGGTGGATCTGAAAGCCTTATGGAGTATGATATTCATTGGTTCCATTCACAGTGAAGTTATTCAGAGAGTATTTACCTTAAAGAGGCTTTTGTTTGTTTTTTGTTCAATTGGCATGGTTTATTGGCTTTTTTGTAATGATTTTGGTTTTAGTTTCCTGTTTTCTTCAGCGACTTACTGATATTTTTTCTGCCTTGTTCTCTACTTATAGAAATCACTAATGTTCAAGTTTGAGAAACAAATTAAGTTTTTCAAAAATTCTCCGAAACTTGATGACAAACAAAAATGTTGTTAATAAAAAACTGTATTTCTTAAGAGAACTGAAAAACAAAATTGTTTGTATTATTCTTACGTAGTGATATGTTAGGATTTGTGATGTACTATTGTTTGGATTACCAGACGAACACTGTGAAATTTTCCCTGAAATGTGTTTGGACTTAATACTAGAAGTTCCAAAATAGAAGTAAAACTTCAAAACTGGGGACTGCTTTTGTGAACAAAGTATTCTGGGCTTTATAAGCAATCATTGGAGTCTGAAAACTGAATGTATAGCAGAAACTTAACAAGAGGAAAAAAAGGCGTTGTCACCATGGTCATGTCTTGAAGGGATTTTCTGTAAATTTGATATTTAAGCAACGGTTCTGAAGTTTGCCTGATGCTCATTGATTGTGTGTAACGCACAAGTTACAGGTGAGGATGGTCAAATTTTTGCTTATAGTGCTAGAATTTTCTAGTAGACATACAAATTCTTTACCACTTGAGGGGCGTTTAGCTCACTATTCTAAAAGTGGGAAGTATAAGTTAGCTAAGCCTAACCCATGGTTGACCCATGGTTGGTTGACAGTGTGAGATCCTCCATCGTTCGGAGAGGGGAATGAAACATTCCTTATATGGGTGTGGAAACCTCTCATTAGTAGACACGTTTTAAAATCTTGAGAGAAAGTCTAGAAGGAAAACTCCAAAAAGGACAATCTTTGCTAGCGGTGGGCTTGGGCTGTTACAAATGTATTAGAGCCAGACATCGAGTAGTGTGCCACGATGCTGGCCCCCAAAGGGGGTGGATTGTGAGATCCCACGTCGTTTGGAGAGGGGAATGAAGTAATGAGCTTGGGCTTGGGCTATTACAAGCAGTAGACGCGTTTTAAAACGTTGACTGGTAGCTTGGAAAGGAAAACCTAAAGAAAACAACATCTGCTAGCAGTGAACTTTGGACTATTACAAACAGTTTTACACGATACATCTTACTCTATTCTACTCCCTAAGTTGCAACAATGCTTTACCTTTACTCACCGGGTTTGCTAGAGTACTTTATCATTAATCTATAGGTTTGCTCCAATATAATTTCTTGTAAATCACTTAAAAATTTCTCTAGTATACTATATGACTGCAAAATCAGCATCTTGAATCACTAATTATTTCCTTGATTGTTTCAGGAGGTAACTGATACATCTGGAGGGTAAGTTCTAAACCACGCGTGATTAAAATTTTGTGATACATTTCTACAGCCATTTTCCTGAAGCATAAAACAAATGCTATCTCCACCAGGTGCGGGGCGAGTTTCGTAATTGATATTGTCTCGGAACAATTCGAAGGGAAAAGGTTGCTTGAACGACATCGTTTGGTGAATGCAGCTCTGGAGGAGGAAATGAAAGAGATTCATGCTCTCTCCATCAAGAAGGCTCTGACCCCACAACAGTGGAAGCAACAGCAAGAAGAATCTGAAAAATCAAAATCTGGTGCTTAGAAGAGCATGATTTATAAGTGAAGTAAGAGACACTGATGATAGTATAAATAAAGCATACTAAATAGCCACTAAAGTAATGGTGTAATTCTGATTCTGCTGTACTTACGTATGAAAATTTCTGCTTTTGAGTTTGTTCTTTGTTGATAGTTGAATTTTAAAAAAATATATATCTCGGATAAAAAAGATTTAGGT

mRNA sequence

CGGCAGGAAGGCAATTGAAGAAAATGGGAGTCACAAAGGAGCAACTCGAATCTACTTTAACCTCCAAATTGAATCCTCAGCATCTGGAGGTAACTGATACATCTGGAGGGTGCGGGGCGAGTTTCGTAATTGATATTGTCTCGGAACAATTCGAAGGGAAAAGGTTGCTTGAACGACATCGTTTGGTGAATGCAGCTCTGGAGGAGGAAATGAAAGAGATTCATGCTCTCTCCATCAAGAAGGCTCTGACCCCACAACAGTGGAAGCAACAGCAAGAAGAATCTGAAAAATCAAAATCTGGTGCTTAGAAGAGCATGATTTATAAGTGAAGTAAGAGACACTGATGATAGTATAAATAAAGCATACTAAATAGCCACTAAAGTAATGGTGTAATTCTGATTCTGCTGTACTTACGTATGAAAATTTCTGCTTTTGAGTTTGTTCTTTGTTGATAGTTGAATTTTAAAAAAATATATATCTCGGATAAAAAAGATTTAGGT

Coding sequence (CDS)

ATGGGAGTCACAAAGGAGCAACTCGAATCTACTTTAACCTCCAAATTGAATCCTCAGCATCTGGAGGTAACTGATACATCTGGAGGGTGCGGGGCGAGTTTCGTAATTGATATTGTCTCGGAACAATTCGAAGGGAAAAGGTTGCTTGAACGACATCGTTTGGTGAATGCAGCTCTGGAGGAGGAAATGAAAGAGATTCATGCTCTCTCCATCAAGAAGGCTCTGACCCCACAACAGTGGAAGCAACAGCAAGAAGAATCTGAAAAATCAAAATCTGGTGCTTAG

Protein sequence

MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALEEEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA

Homology

[Close]

BLAST of Carg24329-RA vs. NCBI nr
Match: KAG6571359.1 (Protein BOLA2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011131.1 Protein BOLA2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 179.5 bits (454), Expect = 1.4e-41
Identity = 94/94 (100.00%), Postives = 94/94 (100.00%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60
          MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE
Sbjct: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60

Query: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 95
          EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA
Sbjct: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 94

[Close]

BLAST of Carg24329-RA vs. NCBI nr
Match: XP_023512401.1 (protein BOLA2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 177.9 bits (450), Expect = 4.0e-41
Identity = 93/94 (98.94%), Postives = 94/94 (100.00%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60
          MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSE+FEGKRLLERHRLVNAALE
Sbjct: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSERFEGKRLLERHRLVNAALE 60

Query: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 95
          EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA
Sbjct: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 94

[Close]

BLAST of Carg24329-RA vs. NCBI nr
Match: XP_022928000.1 (protein BOLA2 [Cucurbita moschata])

HSP 1 Score: 175.6 bits (444), Expect = 2.0e-40
Identity = 91/94 (96.81%), Postives = 94/94 (100.00%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60
          MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSE+FEG+RLLERHRLVNAALE
Sbjct: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSERFEGRRLLERHRLVNAALE 60

Query: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 95
          EEMK+IHALSIKKALTPQQWKQQQEESEKSKSGA
Sbjct: 61 EEMKDIHALSIKKALTPQQWKQQQEESEKSKSGA 94

[Close]

BLAST of Carg24329-RA vs. NCBI nr
Match: XP_022971633.1 (protein BOLA2 [Cucurbita maxima])

HSP 1 Score: 174.1 bits (440), Expect = 5.7e-40
Identity = 91/94 (96.81%), Postives = 92/94 (97.87%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60
          MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSE+FEGKRLLERHRLVNAALE
Sbjct: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSERFEGKRLLERHRLVNAALE 60

Query: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 95
          EEMKEIHALSIKK LTPQQWKQQQEESEKSKS A
Sbjct: 61 EEMKEIHALSIKKVLTPQQWKQQQEESEKSKSAA 94

[Close]

BLAST of Carg24329-RA vs. NCBI nr
Match: XP_004147354.1 (protein BOLA2 [Cucumis sativus] >KGN62134.1 hypothetical protein Csa_006468 [Cucumis sativus])

HSP 1 Score: 173.3 bits (438), Expect = 9.7e-40
Identity = 91/94 (96.81%), Postives = 92/94 (97.87%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60
          MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAAL 
Sbjct: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALV 60

Query: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 95
          EEMK+IHALSIKKALTPQQWKQQQEESEKSKS A
Sbjct: 61 EEMKQIHALSIKKALTPQQWKQQQEESEKSKSAA 94

[Close]

BLAST of Carg24329-RA vs. ExPASy Swiss-Prot
Match: Q9FIC3 (Protein BOLA2 OS=Arabidopsis thaliana OX=3702 GN=BOLA2 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 2.5e-30
Identity = 66/85 (77.65%), Postives = 76/85 (89.41%), Query Frame = 0


Query: 3  VTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALEEE 62
          VTKEQ+E++LTSKL P HLEV D SGGCG+SF +++VSEQFEGKRLLERHR+VNAALEEE
Sbjct: 2  VTKEQVEASLTSKLKPIHLEVIDISGGCGSSFEVEVVSEQFEGKRLLERHRMVNAALEEE 61

Query: 63 MKEIHALSIKKALTPQQWKQQQEES 88
          MKEIHALSIKKA TPQQWK   ++S
Sbjct: 62 MKEIHALSIKKAQTPQQWKPPSQDS 86

[Close]

BLAST of Carg24329-RA vs. ExPASy Swiss-Prot
Match: O14280 (Uncharacterized bolA-like protein C8C9.11 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC8C9.11 PE=3 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 2.1e-13
Identity = 40/82 (48.78%), Postives = 54/82 (65.85%), Query Frame = 0


Query: 3  VTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALEEE 62
          V  +QLE  + + L P H+E+ D SGGCG +F + IVS  FEGK  L RHRLVN  L+E 
Sbjct: 2  VNAQQLELLIQNTLEPTHIEIQDMSGGCGQNFEVIIVSPLFEGKSTLARHRLVNHKLQEV 61

Query: 63 MKEIHALSIKKALTPQQWKQQQ 85
          +K+IHA + +K  +P QW+  Q
Sbjct: 62 IKDIHAFT-QKCYSPAQWEALQ 82

[Close]

BLAST of Carg24329-RA vs. ExPASy Swiss-Prot
Match: Q9H3K6 (BolA-like protein 2 OS=Homo sapiens OX=9606 GN=BOLA2 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 6.8e-12
Identity = 40/87 (45.98%), Postives = 56/87 (64.37%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTS-GGCGASFVIDIVSEQFEGKRLLERHRLVNAAL 60
          M ++ E L   L   L  +H+EV DT+   C  SF + +VS +FEGK LL+RHRLVNA L
Sbjct: 1  MELSAEYLREKLQRDLEAEHVEVEDTTLNRCSCSFRVLVVSAKFEGKPLLQRHRLVNACL 60

Query: 61 EEEMKEIHALSIKKALTPQQWKQQQEE 87
           EE+  IHA   +K LTP QW +++++
Sbjct: 61 AEELPHIHAFE-QKTLTPDQWARERQK 86

[Close]

BLAST of Carg24329-RA vs. ExPASy Swiss-Prot
Match: Q8BGS2 (BolA-like protein 2 OS=Mus musculus OX=10090 GN=Bola2 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 6.8e-12
Identity = 40/87 (45.98%), Postives = 55/87 (63.22%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTS-GGCGASFVIDIVSEQFEGKRLLERHRLVNAAL 60
          M ++ + L   L   L  +H+EV DT+   C  SF + +VS +FEGK LL+RHRLVN  L
Sbjct: 1  MELSADYLREKLRQDLEAEHVEVEDTTLNRCATSFRVLVVSAKFEGKPLLQRHRLVNECL 60

Query: 61 EEEMKEIHALSIKKALTPQQWKQQQEE 87
           EE+  IHA   +K LTP+QW +Q+ E
Sbjct: 61 AEELPHIHAFE-QKTLTPEQWTRQRRE 86

[Close]

BLAST of Carg24329-RA vs. ExPASy Swiss-Prot
Match: Q9USK1 (Uncharacterized bolA-like protein C4B3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC4B3.11c PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-10
Identity = 29/67 (43.28%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 5  KEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALEEEMK 64
          + +++  LT KL+P  L V D SGGCG+ + + I S+ F+GK  L +HRLVN+ L+EE++
Sbjct: 33 ERRIKDILTEKLSPSSLRVIDVSGGCGSMYQVAIKSKAFQGKNTLAQHRLVNSILKEEIR 92

Query: 65 EIHALSI 72
           +H L++
Sbjct: 93 NMHGLNL 99

[Close]

BLAST of Carg24329-RA vs. ExPASy TrEMBL
Match: A0A6J1EQF4 (protein BOLA2 OS=Cucurbita moschata OX=3662 GN=LOC111434902 PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 9.5e-41
Identity = 91/94 (96.81%), Postives = 94/94 (100.00%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60
          MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSE+FEG+RLLERHRLVNAALE
Sbjct: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSERFEGRRLLERHRLVNAALE 60

Query: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 95
          EEMK+IHALSIKKALTPQQWKQQQEESEKSKSGA
Sbjct: 61 EEMKDIHALSIKKALTPQQWKQQQEESEKSKSGA 94

[Close]

BLAST of Carg24329-RA vs. ExPASy TrEMBL
Match: A0A6J1I7F7 (protein BOLA2 OS=Cucurbita maxima OX=3661 GN=LOC111470315 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.8e-40
Identity = 91/94 (96.81%), Postives = 92/94 (97.87%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60
          MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSE+FEGKRLLERHRLVNAALE
Sbjct: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSERFEGKRLLERHRLVNAALE 60

Query: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 95
          EEMKEIHALSIKK LTPQQWKQQQEESEKSKS A
Sbjct: 61 EEMKEIHALSIKKVLTPQQWKQQQEESEKSKSAA 94

[Close]

BLAST of Carg24329-RA vs. ExPASy TrEMBL
Match: A0A0A0LM73 (Transcription regulator OS=Cucumis sativus OX=3659 GN=Csa_2G301520 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.7e-40
Identity = 91/94 (96.81%), Postives = 92/94 (97.87%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60
          MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAAL 
Sbjct: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALV 60

Query: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 95
          EEMK+IHALSIKKALTPQQWKQQQEESEKSKS A
Sbjct: 61 EEMKQIHALSIKKALTPQQWKQQQEESEKSKSAA 94

[Close]

BLAST of Carg24329-RA vs. ExPASy TrEMBL
Match: A0A1S3CEQ9 (protein BOLA2 OS=Cucumis melo OX=3656 GN=LOC103499653 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 8.0e-40
Identity = 91/94 (96.81%), Postives = 91/94 (96.81%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60
          MGVTKEQLESTLTS LNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAAL 
Sbjct: 1  MGVTKEQLESTLTSNLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALV 60

Query: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 95
          EEMKEIHALSIKKALTPQQWKQQQEESEKSKS A
Sbjct: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSAA 94

[Close]

BLAST of Carg24329-RA vs. ExPASy TrEMBL
Match: A0A1R3GCR2 (BolA protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_26948 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.5e-35
Identity = 83/94 (88.30%), Postives = 88/94 (93.62%), Query Frame = 0


Query: 1  MGVTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALE 60
          MGVTKEQ+ES+LTSKLNP HLEV DTSGGCGASF I+IVSEQFEGKRLLERHR+VN ALE
Sbjct: 1  MGVTKEQVESSLTSKLNPSHLEVIDTSGGCGASFAIEIVSEQFEGKRLLERHRIVNGALE 60

Query: 61 EEMKEIHALSIKKALTPQQWKQQQEESEKSKSGA 95
          EEMKEIHALSIKKALTP+QWKQQQ ESEKSKS A
Sbjct: 61 EEMKEIHALSIKKALTPEQWKQQQ-ESEKSKSDA 93

[Close]

BLAST of Carg24329-RA vs. TAIR 10
Match: AT5G09830.1 (BolA-like family protein )

HSP 1 Score: 132.5 bits (332), Expect = 1.8e-31
Identity = 66/85 (77.65%), Postives = 76/85 (89.41%), Query Frame = 0


Query: 3  VTKEQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALEEE 62
          VTKEQ+E++LTSKL P HLEV D SGGCG+SF +++VSEQFEGKRLLERHR+VNAALEEE
Sbjct: 2  VTKEQVEASLTSKLKPIHLEVIDISGGCGSSFEVEVVSEQFEGKRLLERHRMVNAALEEE 61

Query: 63 MKEIHALSIKKALTPQQWKQQQEES 88
          MKEIHALSIKKA TPQQWK   ++S
Sbjct: 62 MKEIHALSIKKAQTPQQWKPPSQDS 86

[Close]

BLAST of Carg24329-RA vs. TAIR 10
Match: AT4G26500.1 (chloroplast sulfur E )

HSP 1 Score: 44.3 bits (103), Expect = 6.4e-05
Identity = 34/94 (36.17%), Postives = 50/94 (53.19%), Query Frame = 0


Query: 1   MGVTKEQLESTLTSKLNPQHLEVTDTS----------GGCG----ASFVIDIVSEQFEGK 60
           +G    ++   L  +L+P  LEV D S          G  G      F + IVS+ F+GK
Sbjct: 278 LGSRGMRIREKLEKELDPVELEVEDVSYQHAGHAAVRGSAGDDGETHFNLRIVSDAFQGK 337

Query: 61  RLLERHRLVNAALEEEMKE-IHALSIKKALTPQQ 80
            L++RHRL+   L++E+K  +HALSI  A TP +
Sbjct: 338 SLVKRHRLIYDLLQDELKSGLHALSI-VAKTPAE 370

[Close]

BLAST of Carg24329-RA vs. TAIR 10
Match: AT5G17560.1 (BolA-like family protein )

HSP 1 Score: 41.6 bits (96), Expect = 4.1e-04
Identity = 25/75 (33.33%), Postives = 42/75 (56.00%), Query Frame = 0


Query: 6   EQLESTLTSKLNPQHLEVTDTSGGCGASFVIDIVSEQFEGKRLLERHRLVNAALEEEMKE 65
           + +E  +  +LN + + VTD SG  G    I++VS  FEG+  + R R+V  A+ EE++ 
Sbjct: 103 QSMELKIKEQLNAESVTVTDMSGD-GRHVCINVVSSAFEGQSAVNRQRMVYKAIWEELQN 162

Query: 66  -IHALSIKKALTPQQ 80
            +HA+      TP +
Sbjct: 163 VVHAVDQMTTKTPSE 176

The following BLAST results are available for this feature:

BLAST of Carg24329-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571359.1	1.4e-41	100.00	Protein BOLA2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011131.1 Pro...	[more]
XP_023512401.1	4.0e-41	98.94	protein BOLA2 [Cucurbita pepo subsp. pepo]	[more]
XP_022928000.1	2.0e-40	96.81	protein BOLA2 [Cucurbita moschata]	[more]
XP_022971633.1	5.7e-40	96.81	protein BOLA2 [Cucurbita maxima]	[more]
XP_004147354.1	9.7e-40	96.81	protein BOLA2 [Cucumis sativus] >KGN62134.1 hypothetical protein Csa_006468 [Cuc...	[more]

BLAST of Carg24329-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FIC3	2.5e-30	77.65	Protein BOLA2 OS=Arabidopsis thaliana OX=3702 GN=BOLA2 PE=1 SV=1	[more]
O14280	2.1e-13	48.78	Uncharacterized bolA-like protein C8C9.11 OS=Schizosaccharomyces pombe (strain 9...	[more]
Q9H3K6	6.8e-12	45.98	BolA-like protein 2 OS=Homo sapiens OX=9606 GN=BOLA2 PE=1 SV=1	[more]
Q8BGS2	6.8e-12	45.98	BolA-like protein 2 OS=Mus musculus OX=10090 GN=Bola2 PE=1 SV=1	[more]
Q9USK1	1.7e-10	43.28	Uncharacterized bolA-like protein C4B3.11c OS=Schizosaccharomyces pombe (strain ...	[more]

BLAST of Carg24329-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EQF4	9.5e-41	96.81	protein BOLA2 OS=Cucurbita moschata OX=3662 GN=LOC111434902 PE=3 SV=1	[more]
A0A6J1I7F7	2.8e-40	96.81	protein BOLA2 OS=Cucurbita maxima OX=3661 GN=LOC111470315 PE=3 SV=1	[more]
A0A0A0LM73	4.7e-40	96.81	Transcription regulator OS=Cucumis sativus OX=3659 GN=Csa_2G301520 PE=3 SV=1	[more]
A0A1S3CEQ9	8.0e-40	96.81	protein BOLA2 OS=Cucumis melo OX=3656 GN=LOC103499653 PE=3 SV=1	[more]
A0A1R3GCR2	3.5e-35	88.30	BolA protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_26948 PE=3 SV=1	[more]

BLAST of Carg24329-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09830.1	1.8e-31	77.65	BolA-like family protein	[more]
AT4G26500.1	6.4e-05	36.17	chloroplast sulfur E	[more]
AT5G17560.1	4.1e-04	33.33	BolA-like family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 49..69
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR12735:SF43	BNAA09G06960D PROTEIN	coord: 1..88
IPR002634	BolA protein	PFAM	PF01722	BolA	coord: 12..78 e-value: 4.2E-20 score: 71.8
IPR002634	BolA protein	PIRSF	PIRSF003113	BolA	coord: 1..94 e-value: 2.5E-32 score: 109.4
IPR036065	BolA-like superfamily	GENE3D	3.30.300.90		coord: 2..93 e-value: 6.4E-35 score: 121.0
IPR036065	BolA-like superfamily	SUPERFAMILY	82657	BolA-like	coord: 1..84
IPR045115	BolA-like protein 2	PANTHER	PTHR12735	BOLA-LIKE PROTEIN-RELATED	coord: 1..88

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg24329	Carg24329	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg24329-RA:3	Carg24329-RA:3	exon
Carg24329-RA:2	Carg24329-RA:2	exon
Carg24329-RA:1	Carg24329-RA:1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg24329-RA:three_prime_utr	Carg24329-RA:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg24329-RA:cds	Carg24329-RA:cds_3	CDS
Carg24329-RA:cds	Carg24329-RA:cds_2	CDS
Carg24329-RA:cds	Carg24329-RA:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg24329-RA:five_prime_utr	Carg24329-RA:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg24329-RA	Carg24329-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
biological_process	GO:0006879	cellular iron ion homeostasis
biological_process	GO:0010039	response to iron ion
biological_process	GO:0006979	response to oxidative stress
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding