Carg24197-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24197-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionTBC1 domain family member 5-like
LocationCarg_Chr12: 8434063 .. 8440820 (-)
Sequence length3073
RNA-Seq ExpressionCarg24197-RA
SyntenyCarg24197-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAAATTCTGGATTATCATTCATTTCATGTGCTGCAGTTTCCTCCAATTTCGTCATTCCCTGTCTTAGGCGTAAGTTTGTCTAAGCCCTTTTGATTTGACTTGTTCCCACCTTCAATTTCTCCTGATCTTTGGTGGGTTTTGCTTTTTTTCTTGATCTTCTCTTCCGATTTCTTCGTTGCAGAATTGCTCGTTTTGATGTGTGGGGGTTTTGTGGGATTTCCTTGACATTTTAGGTTTTTGCTTGTGTTTTTTGTTTGTAGACTCAGAATTTGGAGGATTTGGTTCGAGAAAGTTGAGGTTATATCCATGGCACCTTCTGAAATCGTGGCGGCATTGTCCGAACCGGCTTCGAGGACGCCGTCGTGCTCTGGCTCTGTTTATCATCGGGATTCTGAGGATAAGCGGCGGTTTGTTGATCTTAGAGGTGTTCGGTGGCGTATAAATCTTGGGGTTTTGCCGTCTTCCTCTTTGGCTTCCATTGATCATCTTCGTCGCGTTACAGCTGATTCCAGAAGAAGGTTTGTTTTGCTGGCGTGAATTTAATGTGGCTTGATTTTGTGGATTGTTCTATGATGTTCCTGAAATTCCTTTTCCATTTACTAAAGCTGCTTGTGTATATATATATGATGGATTACGCTGTGGATGAATGTTTCTTCATGTTTTCTTGTTCTGTCTAAACTTTACTTGATTTTTGTTCTTGTGGTTCTTGTTTTTCAAGTTCCCAATTGAGTTTCTTTTGCGAAGTTGAGCTTATAGTTCCATACAGAGGATCTATTGTTTGTCTCATTGAGTTTTGTTTATTTTCGTTTTGTGGGATACGCTAATATGGTGTAGTTGGTGGCTGTAATTTCTGTTTTGAGTTTGACGTCTCCAACTCATTGATTTTAGAGTTTCTTTTGGAAACGTTCTTGTGGAACGTGGGAATATACATGTGTGAGATACTACATCGGTTGGGAGAATGGAACATTCTTTGTAAGGGTGTAAAAACCTCTCCCTAGTAGACGCGCTTTAAAAACCTTGAGAGGAAGCTGGAAGGGAAAAACCGAAGAGGACAATATTTGCTAGCGGTGGGTTTAGGCTGTTACAAACTGTATTAGAGTTAGGCACCGAGCAATGTGCCAGTGAGGAGGCTGAGCCCTGAAGGGGGGTGGACATGAGGCGGTGTGTCAGCGAGGACACTGGGCCCCGAGGGGGTGGATTGGAGAGTCTCAGATTGGAGAAGGGAACGAGTGCCAGTGAGGACACTGGGCCCCGAGGGGGGTGGATTGTGAGATCCCACATTAGTTGGGGAGGAGAACGGAACATTCTTTATAAGGGTGTGGAAACCTCTCTCTTCCTAGTAGACTCATTTTAAAAACCTTGAGGAGAAGCCCAGAAGGGAAAACCGAAGAGGACAATATTTGTTAGCGGTGGGGTTGGACTGTTACAAATGGTATCTGAGCTAGACATGGGCGATGTGTCAGTGAGGAGGCTGAGCCTCAAAGGGGGTGGACACGAGGCGGCGTGCAAGCAAGAACGATAGCCCCAAAGGGAGCTGGATTGTGGGGTTCCACATCTATTGTAGAAGGGAACGAGTGTCAGTAAGGACGCTAGCCCCGAAGGAGAGGTGGATTGTAAGATCCCACATTGGTTGGGGAAGAGAACTAAACCTTCTTTGTAAGGGTGTTGAAACCTCTCCCTAGCATACTCGTTTTAAAAACCTTAAGGAGAAGCCCGGAAGGAAAAGCCAAAGAGGACAATATCTACTAATAGTGAAGTTGAACTGTTACGAATGGTATCAGAGCTAGACATGGACTATGGACAATGTGCTAGTGAGGAGGCTGAGCCTCAAAGGGAGTGGATACGAGGCGGTGTGCCAGCAAGAACGCTAGCCCTAAAGGAGGTGGATTGGGGAGTCCCACATCGATTGGAGAAGGGAACGAGTGCCAGCGAGGAAGCTGGGCCCCGAAGGGGGTGTGGATTTTGAGATCCCACATCGGTTGGGGAGGAGAACGGAATGTTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGACGCCTTTTAAAAACCTTAAGGGGAAGCTCGAAAGGGAAAGTCATAGAGAACAATATCTACTAGCGGTGGAGTTGAGCCGTTACAAATAAATGAATATAATATGAATAATTCGAATTCCTCTTTCTTCTTCATATTTTAATCATCGTATTCAACCTTTTCCTTTCTATGTTTCTTTCAGATATGCCATCTTGAGACGACGCCATCTTGTTGATCCACACATTTCCAAGGATGGAAGCAATTCTCCTGATACAATGGATAATCCACTATCACAAAACCCAGGTATAATGATCTCCAATCTCCTGATACAATGGTTACCTGCCATTGTTGTTCCATTCACATTAAATCGTTCAAGGCGTTCATTTGCGTGCGATATGAGTAGCAATATTTGCTTCTAATTGTCACCCTCTTGTGCTGCAGATAGCTTGTGGGGCCGCTTCTTTCGGAGTGCCGAGCTGGAGAAAATGGTCGACCAGGATTTATCACGTTTATATCCAGAACACGGAAGCTACTTCCAGACGCCCGGATGCCAAGGCTTGCTAAGAAGAATTTTATTGCTATGGTGCCTTCAACATCCGCAGTTCGGTTATAGACAAGGTAATTTCGTAATTTCAGCAATTTACAATGAAGCGAATGATTATTTAAACGGTATGATGAAGATTGTTTAACACTTTCCATGAGAATCTGTCGCATAATCGGCTACTGTTCGTGTTCTTCTTATATTTAAAAGCTATATTCCGTGTTCGATATTGTAGGAATGCATGAACTTTTGGCTCCATTTTTGTACGTTCTTCATGTTGATGTCGAGAGGTTTTCTCAAGTGAGAAAGCTTTATGAAGATCAATTTGCTGATAAGTTTGATGGGGTTTCATTCCAAGATGGTAGTTTCAAATATAATTTTGATTTCAAAAACTGTCTCGATACGACCGAAGATGAGTTGGGAGTTCATGGAAACGAGGAAAATGTGAAAGGTCTTTCCGAGCTAGATCCTGAAATACAAACCATTATATTGTTGACCGATGCGTATGGAGCTGAAGGGGAACTAGGCATCGTCCTTTCCGAACGATTTATAGAACATGACGCGTATACTATGTTCGATGCTTTGATGAGCGGAGCCCATGGGGCAGTTGCTATGGCAGACTTCTTCTCTCCAATGCCTGCAGGTGGTTCTCTTTCGGGATTACCTCCTGTAATCGAAGCATCTGCTGCGTTATACCACTTGCTGTCACATGTCGATTCATCTCTACATGCTCACCTCGTCGAGCTGGGTGTCGAACCACAGTACTTCTTTCTCCGCTGGTTAAGGGTTTTATTCGGTCGTGAATTTTTGCTTGAAGATCTCTTGACGATTTGGGATGAAATATTTGCATCAGATAATAGCAAATTCGACAGAAGTGATGAACCCGAGACAAGCTCCAGCTTCAGCTTCCTTAGCTCGTCTCGTGGTGCGTTTATTGCTGCTATTGCTGTTTCTATGTTACTTTACCTGCGATCGTCTTTACTTGCCACGGAAAATGCTACATTATGTCTCCAGAGATTGTTGAACTTCCCCAAGGATGTGGATTTGTCAAAACTGATAGAGAAAGCAAAATCATGGCAGACGTTCGCAATGCGTTCTAATATCTCATCTACACCATTGTTGTCTGGAGCTTATCACCATCACAGCAAGTCTTTGGTTGCACGAGGTAATGGTCGTTCATCTGGTTCGGTTTCACCGAAAACTCCGTTAAATCAGGTTCCTGAAAGTTACTGGGAAGAGAAGTGGAGAGTGTTACACAGGGAACAAGAATGTAAGCAGAGTGGTTCAAGAACTCAAAATGCAGCACAAAAGAAAGGATGGTCAGAGAAAGTGAGATTCTTGTATAGGACGGAATCTGACCCGTCTCCAGCAAAGCTTGCTGGAGGCAAGAAGAACACGAAGTCCTCCGTTAGGCGAAGGTTGCTGGCAGATTTGGCGCGTGAACTTGGTGCAGAGGAAGACATCGAAAAATGTGGGAGCGATGAAGTACTTGATAAAAAAGATGATATCTCTATAGAAGGGGAGGTGGATGGACACGATGGCTGCGAGAAGTACTCGGAAGATGCCGAAGACAAGAGATTGGAGAGCGGAATTGTTGGCAGTGAAGAAAACTCCTCAGTATTCTCGGACCCAACAAGTTCCTTCAGTGGAGCAAACGATAATGAGAACGACGTAAATGACTCGAGCAGAAGCAGTGTTGCGTCTAGTTTGTCCCCGGATGAAAACGATGACCAGTCACAGTCTGTTGTGGAAGGTTCGCCTGTTCCGGTTCCCGATCAGCTTGAGAACATCCATGAAAAATCTGGGTGCAACAATGATTCCGAGGGCAACGTATCTGTAGGGACAAGGGATAAGAAACTTCTAGGGAAATTTCCTTGGTTTTGGAAGTTTGTCAGGAACGCTTCTAGCGAGGGGAAAGGTGGCGTCGAGGCTTCGAAATCGACTGGAGTTGAGAGTAATCCGATAAAGAATATTGGTTCACCTAAAACTGATGGGGCTTGTAGTACTTCTGGTAGCGGGAAAGGAGATGGTGTGGATCAGAACATGATGGGGACTCTCAAGAATCTTGGTCAATCTATGCTCGAACACATTCAGGTAATATTTCTAAATGTTTGTAACTTCCAAGTTGTTGGGTTCACGTGGACAACTTCTAAGAAGAAGAGGCTCCTATAGATGGAAAATGATCCGAGCTAGACACCGAGTGGTGTGCTAGTGAGGATGCTAGACCCCCAAGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAGCATTCCTTGTAAGGGTGTGGAAACCTCTCCCTAGTAGACAAGTTTTAAAACCTTGAGAAGAAGCCCTTAAAGGGAAATCCCAAAGAGGACAATATTTGCTAGCGGTGGATTTGAGCGGTTACAAATGGTATCATTTGTAACAGAGCCAAACACCGAACAGTGTGCCGCCAGCAAGGACGCTGGGCTCTTAATGGGGTGGATTGTGAGATCCCACATGGGTTAGAGAGGGGAACGACTGGGCTCTTAATGGGGTGGATTGTGAGATCCCACATGGGTTAGAGAGGGGAACGAAGCATCCTTATGAGAGTGTGGAAACCTCTCCCTTTTAGACGCGTTTTAAAAACCTTGAGGGGAAGTTTGAAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGTTGTGGACTTGGGTTGTTATAAATGGTATCAGAGCTAGGCACCGAGTGGTGTGCCAGCGAGTACGCTGGGCTCCTAAAGGGGGTGGATTGTGAGATCCCATATTGGTTGGAGAGGGGAATGAAGCATTCCTTACAAGCGTGTGGAAACCTTTCCTTAGTACATGCGTTTTAAAACCTTGAGGGGAAGTTCTTGAAGGGAAAGCCCAAAAAGGACAATATCTGCTTGCGGTGGGCTTGTGCTATTACTGATGGTATTAGAGTCAGACATCAAGCGGTGTGCCAATGAGGACGCTGAGCTCTTAAGGGGGTAGATTATGAGATCCCACATTGGTTGGAGAGAAGAACGAAGCCTTCCTTATAAGGGTGTGAAAAACTCTCCTTAGTAGACGAGTTTTAAAATCTTGAGGGGAAGCCCTTGAAGGGATAGTTCAAATAGGACAATATTTGCTCGCGGTGGGCTTGGGTTATTACAAATGGTATCAGCGCTAGACACCGAGCGGTATGCCAGCAAGGACGTTAGGCTCCTAAGAGGGGTGGATTGTGAGATCCCACATCAGTTGGAGAGGGGAACAAAACATTCCTTATAAGGGTGTGGAAACCTCTCCTTAGTAGACGCGTTTTAAAACCTTGAGGGAAAGCCCTTGAAGGGAAAGCTGAAAGAGGACAATATCTGCTAGTGGGCTAAAGTTTGCCCCTCCCTAAGACAATTGGTGGATCCTCCCCTAGCGAGAGTCTTACCCTTATTATAGCTAACACGACCCGAAAATGAATGATTATGGTTATTGGGACGACGAACTTTAGCGTAATTTGTGTACGTTTGTCATCCATTCGTACAATAGCTGACAATTTGGTCGACAGGTTATCGAAACCGTATTCCAGCAAGATCGGGTTCAAGTAGGATCGCTGGAGAACTTGTCGAAGAACCCTTTAGTTGGCAAAGGACAAGTAACAGCCATGGCAGCTCTGAAGGAGCTTCGGAAGATCAGCAATCTCCTATCTGAGATGTAAGACACTTCTATTCTTGCTGTATCTGTATATACAATTTGTAATTATATTTTTAGTGGCGTAATGATATTGAGTTTTATCTGTACTACCCCCCTCTTTCGATACGTCGAGTTAAAATCTGTAGTGTCGTTACAACGACACGACACCGAGCTTTTGTACTGAGGAGGCTAAGCTAGAATAAAACGGATACAAAATTTTGTTCCAGTTACCTGTTCTTTCTGAGCATTAGGGGGCTCCGTTCGTGTTGTTCTGGTTGAAATTGTTTGTAAAGTGGCTTGAGAACATCGACCTTGAAGACAAGGTAATTGTTATTAGTATTTGCAGATTGAGTTATGCTTAAGTTGACATTATCACGTTTTAAGGTATCACATTTTTCTTTTAGATCAAATGTTTAAATTTTTCGGTTACTAAGAACCAAGAACGCAATCAACCCAACTTAACTCGAACCAAATAACTGGATAAGACAAGGGGATTGTTATTAGTATCTGCAGATTGAACTATACTTAAGTTGACATTATCACGTTT

mRNA sequence

ATGATGAAATTCTGGATTATCATTCATTTCATACTCAGAATTTGGAGGATTTGGTTCGAGAAAGTTGAGGTTATATCCATGGCACCTTCTGAAATCGTGGCGGCATTGTCCGAACCGGCTTCGAGGACGCCGTCGTGCTCTGGCTCTGTTTATCATCGGGATTCTGAGGATAAGCGGCGGTTTGTTGATCTTAGAGGTGTTCGGTGGCGTATAAATCTTGGGGTTTTGCCGTCTTCCTCTTTGGCTTCCATTGATCATCTTCGTCGCGTTACAGCTGATTCCAGAAGAAGATATGCCATCTTGAGACGACGCCATCTTGTTGATCCACACATTTCCAAGGATGGAAGCAATTCTCCTGATACAATGGATAATCCACTATCACAAAACCCAGATAGCTTGTGGGGCCGCTTCTTTCGGAGTGCCGAGCTGGAGAAAATGGTCGACCAGGATTTATCACGTTTATATCCAGAACACGGAAGCTACTTCCAGACGCCCGGATGCCAAGGCTTGCTAAGAAGAATTTTATTGCTATGGTGCCTTCAACATCCGCAGTTCGGTTATAGACAAGGAATGCATGAACTTTTGGCTCCATTTTTGTACGTTCTTCATGTTGATGTCGAGAGGTTTTCTCAAGTGAGAAAGCTTTATGAAGATCAATTTGCTGATAAGTTTGATGGGGTTTCATTCCAAGATGGTAGTTTCAAATATAATTTTGATTTCAAAAACTGTCTCGATACGACCGAAGATGAGTTGGGAGTTCATGGAAACGAGGAAAATGTGAAAGGTCTTTCCGAGCTAGATCCTGAAATACAAACCATTATATTGTTGACCGATGCGTATGGAGCTGAAGGGGAACTAGGCATCGTCCTTTCCGAACGATTTATAGAACATGACGCGTATACTATGTTCGATGCTTTGATGAGCGGAGCCCATGGGGCAGTTGCTATGGCAGACTTCTTCTCTCCAATGCCTGCAGGTGGTTCTCTTTCGGGATTACCTCCTGTAATCGAAGCATCTGCTGCGTTATACCACTTGCTGTCACATGTCGATTCATCTCTACATGCTCACCTCGTCGAGCTGGGTGTCGAACCACAGTACTTCTTTCTCCGCTGGTTAAGGGTTTTATTCGGTCGTGAATTTTTGCTTGAAGATCTCTTGACGATTTGGGATGAAATATTTGCATCAGATAATAGCAAATTCGACAGAAGTGATGAACCCGAGACAAGCTCCAGCTTCAGCTTCCTTAGCTCGTCTCGTGGTGCGTTTATTGCTGCTATTGCTGTTTCTATGTTACTTTACCTGCGATCGTCTTTACTTGCCACGGAAAATGCTACATTATGTCTCCAGAGATTGTTGAACTTCCCCAAGGATGTGGATTTGTCAAAACTGATAGAGAAAGCAAAATCATGGCAGACGTTCGCAATGCGTTCTAATATCTCATCTACACCATTGTTGTCTGGAGCTTATCACCATCACAGCAAGTCTTTGGTTGCACGAGGTAATGGTCGTTCATCTGGTTCGGTTTCACCGAAAACTCCGTTAAATCAGGTTCCTGAAAGTTACTGGGAAGAGAAGTGGAGAGTGTTACACAGGGAACAAGAATGTAAGCAGAGTGGTTCAAGAACTCAAAATGCAGCACAAAAGAAAGGATGGTCAGAGAAAGTGAGATTCTTGTATAGGACGGAATCTGACCCGTCTCCAGCAAAGCTTGCTGGAGGCAAGAAGAACACGAAGTCCTCCGTTAGGCGAAGGTTGCTGGCAGATTTGGCGCGTGAACTTGGTGCAGAGGAAGACATCGAAAAATGTGGGAGCGATGAAGTACTTGATAAAAAAGATGATATCTCTATAGAAGGGGAGGTGGATGGACACGATGGCTGCGAGAAGTACTCGGAAGATGCCGAAGACAAGAGATTGGAGAGCGGAATTGTTGGCAGTGAAGAAAACTCCTCAGTATTCTCGGACCCAACAAGTTCCTTCAGTGGAGCAAACGATAATGAGAACGACGTAAATGACTCGAGCAGAAGCAGTGTTGCGTCTAGTTTGTCCCCGGATGAAAACGATGACCAGTCACAGTCTGTTGTGGAAGGTTCGCCTGTTCCGGTTCCCGATCAGCTTGAGAACATCCATGAAAAATCTGGGTGCAACAATGATTCCGAGGGCAACGTATCTGTAGGGACAAGGGATAAGAAACTTCTAGGGAAATTTCCTTGGTTTTGGAAGTTTGTCAGGAACGCTTCTAGCGAGGGGAAAGGTGGCGTCGAGGCTTCGAAATCGACTGGAGTTGAGAGTAATCCGATAAAGAATATTGGTTCACCTAAAACTGATGGGGCTTGTAGTACTTCTGGTAGCGGGAAAGGAGATGGTGTGGATCAGAACATGATGGGGACTCTCAAGAATCTTGGTTATCGAAACCGTATTCCAGCAAGATCGGGTTCAAGTAGGATCGCTGGAGAACTTGTCGAAGAACCCTTTAGTTGGCAAAGGACAAGTAACAGCCATGGCAGCTCTGAAGGAGCTTCGGAAGATCAGCAATCTCCTATCTGAGATGTAAGACACTTCTATTCTTGCTGTATCTGTATATACAATTTGTAATTATATTTTTAGTGGCGTAATGATATTGAGTTTTATCTGTACTACCCCCCTCTTTCGATACGTCGAGTTAAAATCTGTAGTGTCGTTACAACGACACGACACCGAGCTTTTGTACTGAGGAGGCTAAGCTAGAATAAAACGGATACAAAATTTTGTTCCAGTTACCTGTTCTTTCTGAGCATTAGGGGGCTCCGTTCGTGTTGTTCTGGTTGAAATTGTTTGTAAAGTGGCTTGAGAACATCGACCTTGAAGACAAGGTAATTGTTATTAGTATTTGCAGATTGAGTTATGCTTAAGTTGACATTATCACGTTTTAAGGTATCACATTTTTCTTTTAGATCAAATGTTTAAATTTTTCGGTTACTAAGAACCAAGAACGCAATCAACCCAACTTAACTCGAACCAAATAACTGGATAAGACAAGGGGATTGTTATTAGTATCTGCAGATTGAACTATACTTAAGTTGACATTATCACGTTT

Coding sequence (CDS)

ATGATGAAATTCTGGATTATCATTCATTTCATACTCAGAATTTGGAGGATTTGGTTCGAGAAAGTTGAGGTTATATCCATGGCACCTTCTGAAATCGTGGCGGCATTGTCCGAACCGGCTTCGAGGACGCCGTCGTGCTCTGGCTCTGTTTATCATCGGGATTCTGAGGATAAGCGGCGGTTTGTTGATCTTAGAGGTGTTCGGTGGCGTATAAATCTTGGGGTTTTGCCGTCTTCCTCTTTGGCTTCCATTGATCATCTTCGTCGCGTTACAGCTGATTCCAGAAGAAGATATGCCATCTTGAGACGACGCCATCTTGTTGATCCACACATTTCCAAGGATGGAAGCAATTCTCCTGATACAATGGATAATCCACTATCACAAAACCCAGATAGCTTGTGGGGCCGCTTCTTTCGGAGTGCCGAGCTGGAGAAAATGGTCGACCAGGATTTATCACGTTTATATCCAGAACACGGAAGCTACTTCCAGACGCCCGGATGCCAAGGCTTGCTAAGAAGAATTTTATTGCTATGGTGCCTTCAACATCCGCAGTTCGGTTATAGACAAGGAATGCATGAACTTTTGGCTCCATTTTTGTACGTTCTTCATGTTGATGTCGAGAGGTTTTCTCAAGTGAGAAAGCTTTATGAAGATCAATTTGCTGATAAGTTTGATGGGGTTTCATTCCAAGATGGTAGTTTCAAATATAATTTTGATTTCAAAAACTGTCTCGATACGACCGAAGATGAGTTGGGAGTTCATGGAAACGAGGAAAATGTGAAAGGTCTTTCCGAGCTAGATCCTGAAATACAAACCATTATATTGTTGACCGATGCGTATGGAGCTGAAGGGGAACTAGGCATCGTCCTTTCCGAACGATTTATAGAACATGACGCGTATACTATGTTCGATGCTTTGATGAGCGGAGCCCATGGGGCAGTTGCTATGGCAGACTTCTTCTCTCCAATGCCTGCAGGTGGTTCTCTTTCGGGATTACCTCCTGTAATCGAAGCATCTGCTGCGTTATACCACTTGCTGTCACATGTCGATTCATCTCTACATGCTCACCTCGTCGAGCTGGGTGTCGAACCACAGTACTTCTTTCTCCGCTGGTTAAGGGTTTTATTCGGTCGTGAATTTTTGCTTGAAGATCTCTTGACGATTTGGGATGAAATATTTGCATCAGATAATAGCAAATTCGACAGAAGTGATGAACCCGAGACAAGCTCCAGCTTCAGCTTCCTTAGCTCGTCTCGTGGTGCGTTTATTGCTGCTATTGCTGTTTCTATGTTACTTTACCTGCGATCGTCTTTACTTGCCACGGAAAATGCTACATTATGTCTCCAGAGATTGTTGAACTTCCCCAAGGATGTGGATTTGTCAAAACTGATAGAGAAAGCAAAATCATGGCAGACGTTCGCAATGCGTTCTAATATCTCATCTACACCATTGTTGTCTGGAGCTTATCACCATCACAGCAAGTCTTTGGTTGCACGAGGTAATGGTCGTTCATCTGGTTCGGTTTCACCGAAAACTCCGTTAAATCAGGTTCCTGAAAGTTACTGGGAAGAGAAGTGGAGAGTGTTACACAGGGAACAAGAATGTAAGCAGAGTGGTTCAAGAACTCAAAATGCAGCACAAAAGAAAGGATGGTCAGAGAAAGTGAGATTCTTGTATAGGACGGAATCTGACCCGTCTCCAGCAAAGCTTGCTGGAGGCAAGAAGAACACGAAGTCCTCCGTTAGGCGAAGGTTGCTGGCAGATTTGGCGCGTGAACTTGGTGCAGAGGAAGACATCGAAAAATGTGGGAGCGATGAAGTACTTGATAAAAAAGATGATATCTCTATAGAAGGGGAGGTGGATGGACACGATGGCTGCGAGAAGTACTCGGAAGATGCCGAAGACAAGAGATTGGAGAGCGGAATTGTTGGCAGTGAAGAAAACTCCTCAGTATTCTCGGACCCAACAAGTTCCTTCAGTGGAGCAAACGATAATGAGAACGACGTAAATGACTCGAGCAGAAGCAGTGTTGCGTCTAGTTTGTCCCCGGATGAAAACGATGACCAGTCACAGTCTGTTGTGGAAGGTTCGCCTGTTCCGGTTCCCGATCAGCTTGAGAACATCCATGAAAAATCTGGGTGCAACAATGATTCCGAGGGCAACGTATCTGTAGGGACAAGGGATAAGAAACTTCTAGGGAAATTTCCTTGGTTTTGGAAGTTTGTCAGGAACGCTTCTAGCGAGGGGAAAGGTGGCGTCGAGGCTTCGAAATCGACTGGAGTTGAGAGTAATCCGATAAAGAATATTGGTTCACCTAAAACTGATGGGGCTTGTAGTACTTCTGGTAGCGGGAAAGGAGATGGTGTGGATCAGAACATGATGGGGACTCTCAAGAATCTTGGTTATCGAAACCGTATTCCAGCAAGATCGGGTTCAAGTAGGATCGCTGGAGAACTTGTCGAAGAACCCTTTAGTTGGCAAAGGACAAGTAACAGCCATGGCAGCTCTGAAGGAGCTTCGGAAGATCAGCAATCTCCTATCTGA

Protein sequence

MMKFWIIIHFILRIWRIWFEKVEVISMAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAGGSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLLTIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSGSVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPSPAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGCEKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSPDENDDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNASSEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLGYRNRIPARSGSSRIAGELVEEPFSWQRTSNSHGSSEGASEDQQSPI
Homology
BLAST of Carg24197-RA vs. NCBI nr
Match: KAG7020929.1 (TBC1 domain family member 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1655.6 bits (4286), Expect = 0.0e+00
Identity = 847/847 (100.00%), Postives = 847/847 (100.00%), Query Frame = 0

Query: 1   MMKFWIIIHFILRIWRIWFEKVEVISMAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRR 60
           MMKFWIIIHFILRIWRIWFEKVEVISMAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRR
Sbjct: 1   MMKFWIIIHFILRIWRIWFEKVEVISMAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRR 60

Query: 61  FVDLRGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSPD 120
           FVDLRGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSPD
Sbjct: 61  FVDLRGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSPD 120

Query: 121 TMDNPLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCL 180
           TMDNPLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCL
Sbjct: 121 TMDNPLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCL 180

Query: 181 QHPQFGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDF 240
           QHPQFGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDF
Sbjct: 181 QHPQFGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDF 240

Query: 241 KNCLDTTEDELGVHGNEENVKGLSELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAY 300
           KNCLDTTEDELGVHGNEENVKGLSELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAY
Sbjct: 241 KNCLDTTEDELGVHGNEENVKGLSELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAY 300

Query: 301 TMFDALMSGAHGAVAMADFFSPMPAGGSLSGLPPVIEASAALYHLLSHVDSSLHAHLVEL 360
           TMFDALMSGAHGAVAMADFFSPMPAGGSLSGLPPVIEASAALYHLLSHVDSSLHAHLVEL
Sbjct: 301 TMFDALMSGAHGAVAMADFFSPMPAGGSLSGLPPVIEASAALYHLLSHVDSSLHAHLVEL 360

Query: 361 GVEPQYFFLRWLRVLFGREFLLEDLLTIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRG 420
           GVEPQYFFLRWLRVLFGREFLLEDLLTIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRG
Sbjct: 361 GVEPQYFFLRWLRVLFGREFLLEDLLTIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRG 420

Query: 421 AFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNIS 480
           AFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNIS
Sbjct: 421 AFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNIS 480

Query: 481 STPLLSGAYHHHSKSLVARGNGRSSGSVSPKTPLNQVPESYWEEKWRVLHREQECKQSGS 540
           STPLLSGAYHHHSKSLVARGNGRSSGSVSPKTPLNQVPESYWEEKWRVLHREQECKQSGS
Sbjct: 481 STPLLSGAYHHHSKSLVARGNGRSSGSVSPKTPLNQVPESYWEEKWRVLHREQECKQSGS 540

Query: 541 RTQNAAQKKGWSEKVRFLYRTESDPSPAKLAGGKKNTKSSVRRRLLADLARELGAEEDIE 600
           RTQNAAQKKGWSEKVRFLYRTESDPSPAKLAGGKKNTKSSVRRRLLADLARELGAEEDIE
Sbjct: 541 RTQNAAQKKGWSEKVRFLYRTESDPSPAKLAGGKKNTKSSVRRRLLADLARELGAEEDIE 600

Query: 601 KCGSDEVLDKKDDISIEGEVDGHDGCEKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFS 660
           KCGSDEVLDKKDDISIEGEVDGHDGCEKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFS
Sbjct: 601 KCGSDEVLDKKDDISIEGEVDGHDGCEKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFS 660

Query: 661 GANDNENDVNDSSRSSVASSLSPDENDDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEG 720
           GANDNENDVNDSSRSSVASSLSPDENDDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEG
Sbjct: 661 GANDNENDVNDSSRSSVASSLSPDENDDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEG 720

Query: 721 NVSVGTRDKKLLGKFPWFWKFVRNASSEGKGGVEASKSTGVESNPIKNIGSPKTDGACST 780
           NVSVGTRDKKLLGKFPWFWKFVRNASSEGKGGVEASKSTGVESNPIKNIGSPKTDGACST
Sbjct: 721 NVSVGTRDKKLLGKFPWFWKFVRNASSEGKGGVEASKSTGVESNPIKNIGSPKTDGACST 780

Query: 781 SGSGKGDGVDQNMMGTLKNLGYRNRIPARSGSSRIAGELVEEPFSWQRTSNSHGSSEGAS 840
           SGSGKGDGVDQNMMGTLKNLGYRNRIPARSGSSRIAGELVEEPFSWQRTSNSHGSSEGAS
Sbjct: 781 SGSGKGDGVDQNMMGTLKNLGYRNRIPARSGSSRIAGELVEEPFSWQRTSNSHGSSEGAS 840

Query: 841 EDQQSPI 848
           EDQQSPI
Sbjct: 841 EDQQSPI 847

BLAST of Carg24197-RA vs. NCBI nr
Match: XP_022937789.1 (TBC1 domain family member 5-like [Cucurbita moschata] >XP_022937791.1 TBC1 domain family member 5-like [Cucurbita moschata] >XP_022937792.1 TBC1 domain family member 5-like [Cucurbita moschata])

HSP 1 Score: 1493.8 bits (3866), Expect = 0.0e+00
Identity = 765/775 (98.71%), Postives = 768/775 (99.10%), Query Frame = 0

Query: 27  MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 86
           MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH
Sbjct: 1   MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 60

Query: 87  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 146
           LRRVTADSRRRYAILRRRHLVDPHISK+GSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM
Sbjct: 61  LRRVTADSRRRYAILRRRHLVDPHISKEGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 120

Query: 147 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 206
           VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV
Sbjct: 121 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 180

Query: 207 ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL 266
           ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL
Sbjct: 181 ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL 240

Query: 267 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 326
           DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMF+ALMSGAHGAVAMADFFSPMPAG
Sbjct: 241 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFEALMSGAHGAVAMADFFSPMPAG 300

Query: 327 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 386
           GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL
Sbjct: 301 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 360

Query: 387 TIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 446
           TIWDEIFASDNSKFDRSDEPETSSSF FLSSSRGAFIAAIAVSMLLYLRSSLLATENATL
Sbjct: 361 TIWDEIFASDNSKFDRSDEPETSSSFDFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 420

Query: 447 CLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 506
           CLQRLLNFPKDVDL KLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGN RSSG
Sbjct: 421 CLQRLLNFPKDVDLPKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNDRSSG 480

Query: 507 SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 566
           SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVR LYRTESDPS
Sbjct: 481 SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRSLYRTESDPS 540

Query: 567 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC 626
           PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC
Sbjct: 541 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC 600

Query: 627 EKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSPDEN 686
           EKYSE+AED RLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLS DEN
Sbjct: 601 EKYSENAEDNRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSLDEN 660

Query: 687 DDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 746
           DDQSQSVVEGSPVPVPDQLENI EKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS
Sbjct: 661 DDQSQSVVEGSPVPVPDQLENIQEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 720

Query: 747 SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 802
           SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG
Sbjct: 721 SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 775

BLAST of Carg24197-RA vs. NCBI nr
Match: XP_023537563.1 (TBC1 domain family member 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1486.1 bits (3846), Expect = 0.0e+00
Identity = 762/775 (98.32%), Postives = 766/775 (98.84%), Query Frame = 0

Query: 27  MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 86
           MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH
Sbjct: 1   MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 60

Query: 87  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 146
           LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM
Sbjct: 61  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 120

Query: 147 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 206
           VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV
Sbjct: 121 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 180

Query: 207 ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL 266
           ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTT+DELGVHGNEENVKGLSEL
Sbjct: 181 ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTKDELGVHGNEENVKGLSEL 240

Query: 267 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 326
           DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG
Sbjct: 241 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 300

Query: 327 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 386
           GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL
Sbjct: 301 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 360

Query: 387 TIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 446
           TIWDEIFASDNSKFDRSDEPETSSSF FLSSSRGAFIAAIAVSMLLYLRSSLLATENATL
Sbjct: 361 TIWDEIFASDNSKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 420

Query: 447 CLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 506
           CLQRLLNFPKDVDL KLIEKAKSWQT AMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG
Sbjct: 421 CLQRLLNFPKDVDLPKLIEKAKSWQTLAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 480

Query: 507 SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 566
           SVSPKTPLN VPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS
Sbjct: 481 SVSPKTPLNHVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 540

Query: 567 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC 626
           PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEK  SDEVLDKKDDISIEGEVDGHDGC
Sbjct: 541 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKSRSDEVLDKKDDISIEGEVDGHDGC 600

Query: 627 EKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSPDEN 686
           E YSE+AEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLS DEN
Sbjct: 601 ENYSENAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSLDEN 660

Query: 687 DDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 746
           DDQSQSVVEGSP+PVPDQLENI EKSGCN+DSEGNVSVGTRDKKLLGKFPWFWKFVRNAS
Sbjct: 661 DDQSQSVVEGSPLPVPDQLENIPEKSGCNHDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 720

Query: 747 SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 802
           SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG
Sbjct: 721 SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 775

BLAST of Carg24197-RA vs. NCBI nr
Match: XP_022966053.1 (TBC1 domain family member 5-like isoform X1 [Cucurbita maxima] >XP_022966054.1 TBC1 domain family member 5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 756/775 (97.55%), Postives = 763/775 (98.45%), Query Frame = 0

Query: 27  MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 86
           MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSS ASIDH
Sbjct: 1   MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSSASIDH 60

Query: 87  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 146
           LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM
Sbjct: 61  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 120

Query: 147 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 206
           VDQDLSRLYPEHGSYFQT GCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV
Sbjct: 121 VDQDLSRLYPEHGSYFQTSGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 180

Query: 207 ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL 266
            RFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLD T+DELGVHGNEENVKGLSEL
Sbjct: 181 VRFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDMTDDELGVHGNEENVKGLSEL 240

Query: 267 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 326
           DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG
Sbjct: 241 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 300

Query: 327 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 386
           GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL
Sbjct: 301 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 360

Query: 387 TIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 446
           TIWDEIFASDNSKFDRSDEPETSSSF FLSSSRGAFIAAIAVSMLLYLRSSLLATENATL
Sbjct: 361 TIWDEIFASDNSKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 420

Query: 447 CLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 506
           CLQRLLNFPKDVDL KLIEKAKSWQT AMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG
Sbjct: 421 CLQRLLNFPKDVDLPKLIEKAKSWQTLAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 480

Query: 507 SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 566
           SVSPKTPLN VPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS
Sbjct: 481 SVSPKTPLNHVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 540

Query: 567 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC 626
           PAKLAGGKKNTKSSVRRRLLADLARELGAEED++KC S+EVLDKKDDISIEGEVDGHDGC
Sbjct: 541 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDLKKCESNEVLDKKDDISIEGEVDGHDGC 600

Query: 627 EKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSPDEN 686
           EKYSE+AEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLS DEN
Sbjct: 601 EKYSENAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSLDEN 660

Query: 687 DDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 746
           DDQSQSVVEGSP+PVPDQLENI EKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS
Sbjct: 661 DDQSQSVVEGSPLPVPDQLENIPEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 720

Query: 747 SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 802
           SEGKGGVE+SKSTGVESNPIKNI SPKTDGACSTSGSGKGDGVDQNMMGTLKNLG
Sbjct: 721 SEGKGGVESSKSTGVESNPIKNIASPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 775

BLAST of Carg24197-RA vs. NCBI nr
Match: XP_022966055.1 (TBC1 domain family member 5-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 756/775 (97.55%), Postives = 763/775 (98.45%), Query Frame = 0

Query: 27  MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 86
           MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSS ASIDH
Sbjct: 1   MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSSASIDH 60

Query: 87  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 146
           LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM
Sbjct: 61  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 120

Query: 147 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 206
           VDQDLSRLYPEHGSYFQT GCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV
Sbjct: 121 VDQDLSRLYPEHGSYFQTSGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 180

Query: 207 ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL 266
            RFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLD T+DELGVHGNEENVKGLSEL
Sbjct: 181 VRFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDMTDDELGVHGNEENVKGLSEL 240

Query: 267 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 326
           DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG
Sbjct: 241 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 300

Query: 327 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 386
           GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL
Sbjct: 301 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 360

Query: 387 TIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 446
           TIWDEIFASDNSKFDRSDEPETSSSF FLSSSRGAFIAAIAVSMLLYLRSSLLATENATL
Sbjct: 361 TIWDEIFASDNSKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 420

Query: 447 CLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 506
           CLQRLLNFPKDVDL KLIEKAKSWQT AMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG
Sbjct: 421 CLQRLLNFPKDVDLPKLIEKAKSWQTLAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 480

Query: 507 SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 566
           SVSPKTPLN VPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS
Sbjct: 481 SVSPKTPLNHVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 540

Query: 567 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC 626
           PAKLAGGKKNTKSSVRRRLLADLARELGAEED++KC S+EVLDKKDDISIEGEVDGHDGC
Sbjct: 541 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDLKKCESNEVLDKKDDISIEGEVDGHDGC 600

Query: 627 EKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSPDEN 686
           EKYSE+AEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLS DEN
Sbjct: 601 EKYSENAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSLDEN 660

Query: 687 DDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 746
           DDQSQSVVEGSP+PVPDQLENI EKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS
Sbjct: 661 DDQSQSVVEGSPLPVPDQLENIPEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 720

Query: 747 SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 802
           SEGKGGVE+SKSTGVESNPIKNI SPKTDGACSTSGSGKGDGVDQNMMGTLKNLG
Sbjct: 721 SEGKGGVESSKSTGVESNPIKNIASPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 775

BLAST of Carg24197-RA vs. ExPASy Swiss-Prot
Match: Q80XQ2 (TBC1 domain family member 5 OS=Mus musculus OX=10090 GN=Tbc1d5 PE=1 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 1.7e-32
Identity = 114/418 (27.27%), Postives = 182/418 (43.54%), Query Frame = 0

Query: 65  RGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDN 124
           R + W++ L VLP      I  ++ +    R  Y+ ++  H+ +P   K        ++N
Sbjct: 87  RSICWKLFLCVLPQDKSQWISKIKEL----RAWYSSIKEIHITNPR--KAAGQQDLMINN 146

Query: 125 PLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQ 184
           PLSQ+  SLW +FF+  EL  M++QD+ R +PE   +FQ    + +L  +L  +  ++ Q
Sbjct: 147 PLSQDEGSLWNKFFQDKELRSMIEQDVKRTFPEM-QFFQQENVRKILTDVLFCYARENEQ 206

Query: 185 FGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCL 244
             Y+QGMHELLAP ++ LH D + F                                   
Sbjct: 207 LLYKQGMHELLAPIIFTLHCDHQAF----------------------------------- 266

Query: 245 DTTEDELGVHGNEENVKGLSELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFD 304
                   +H +E      ++   E++T+                L+  ++EHDAY MF 
Sbjct: 267 --------LHASES-----AQPSEEMKTL----------------LNPEYLEHDAYAMFS 326

Query: 305 ALM---------------SGAHGAVAMADFFSPMPAGGSLSGLPPVIEASAALYHLLSHV 364
            LM                G    +A   F  P   G +++    V + +    HLL   
Sbjct: 327 QLMETAEPWFSTFEHDGQKGKETLMAPIPFARPQDLGPTVA---IVTKVNQIQDHLLKKH 386

Query: 365 DSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLLTIWDEIFASDNSKFDRSDEPETS 424
           D  L+ HL  L + PQ + LRW+R+LFGREF L+DLL +WD +FA               
Sbjct: 387 DIELYMHLNRLEIAPQIYGLRWVRLLFGREFPLQDLLVVWDALFA--------------- 408

Query: 425 SSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKDVDLSKLIEKA 468
                  S   + +  +  +MLLY+R +L+++ N   CL  L+++P   D+  LI KA
Sbjct: 447 ------DSLNLSLVDYVFTAMLLYIRDALISS-NYQTCLGLLMHYPIIGDIHSLILKA 408

BLAST of Carg24197-RA vs. ExPASy Swiss-Prot
Match: Q54VM3 (TBC1 domain family member 5 homolog A OS=Dictyostelium discoideum OX=44689 GN=tbc1d5A PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 7.7e-30
Identity = 116/444 (26.13%), Postives = 201/444 (45.27%), Query Frame = 0

Query: 64  LRGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDGS------- 123
           +R + WRI LG L       ++     T  SR++Y   ++ ++++P  SKD         
Sbjct: 379 VRSIFWRIALGTLSKDPTTWVER----TNSSRKKYETFKKNYIINPRNSKDQDADLQQQQ 438

Query: 124 --------NSPDTMDNPLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQ 183
                        +D+PLSQ+ DSLW +FF +   ++ +  D+SR YP  G +F+    Q
Sbjct: 439 QQQQQQQRKPVSLIDDPLSQSEDSLWNQFFDNENAQREISHDISRTYPGLG-FFERLDIQ 498

Query: 184 GLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADK-FDGV 243
            ++ RIL ++  Q+P+  Y QGM+E+LAP LY ++ D   F+       D F+ K +D  
Sbjct: 499 DIMIRILFIFSKQYPKIKYLQGMNEILAPILYSVYNDSHWFNN-----RDVFSKKNYDKK 558

Query: 244 SFQDGSFKYNFDFKNCLDTTED---ELGVHGNEENVKGLSELDPEIQTIILLTDAYGAEG 303
           + Q   F + FD +   D   D   +   + N  N  G S     +            +G
Sbjct: 559 NKQYEHFDFVFDQQYQQDYYPDGPIQYPTNSNNFNGAGSSGSGGSVSR---------KDG 618

Query: 304 ELGIVLSE-RFIEHDAYTMFDALMSGAHGAVAMADFFSPMP---AGGSLSGLPPVIEASA 363
            +G  L + ++ EHD+Y +F++LM+   G    +   SP P     G    L  + E  A
Sbjct: 619 GIGAFLRDPQYFEHDSYFIFESLMT-IVGKWFTSPPSSPQPPPRVQGQFKKLYDLSERDA 678

Query: 364 A--------------LYHLLSHVDSSLHAHL-VELGVEPQYFFLRWLRVLFGREFLLEDL 423
           +              ++  L  ++  L+++L  +LG+EP  + LRW+R++  + F L+ L
Sbjct: 679 SDQAVNIVVVDQCLRMFEDLKFIEPQLYSYLKQDLGIEPHLYSLRWIRIILAQVFPLDSL 738

Query: 424 LTIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENAT 470
           L +WD IF                        S   F+  I ++ML+ ++  ++  + + 
Sbjct: 739 LILWDSIF----------------------KESVTEFLPYICLTMLIMIKDQIIEKDYSE 779

BLAST of Carg24197-RA vs. ExPASy Swiss-Prot
Match: Q54TA5 (TBC1 domain family member 5 homolog B OS=Dictyostelium discoideum OX=44689 GN=tbc1d5B PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.1e-28
Identity = 115/443 (25.96%), Postives = 188/443 (42.44%), Query Frame = 0

Query: 60  RFVDLRGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSP 119
           ++  LRG+ W++ LG L    +  +D   R     R+RY  L+  H  DP       NS 
Sbjct: 281 KYSPLRGIAWKLFLGGL---DINRVDKWERDITQQRKRYEKLKEEHCFDPR------NSN 340

Query: 120 DTMDNPLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWC 179
            T D PLSQN DS W +FF++ + +K++  DL R +P++  +F  P  + ++  IL ++ 
Sbjct: 341 STYD-PLSQNDDSPWNKFFKNLDTQKIIKIDLERTHPDN-DFFSNPVIREMMATILFVYS 400

Query: 180 LQHPQFGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFAD-----------KFDGVS 239
             +    YRQGMHELLAP +Y+ +   + +S  +KL E+  +            + D  +
Sbjct: 401 KTNGIISYRQGMHELLAPIIYLYN---QEYSSYKKLDENSSSTLVDFIYNIKYLEHDTFA 460

Query: 240 FQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSELDPEIQTIILLTDAYGAEGELGI 299
             +   K+  D+     T +     + N  N    +       +    +    A   +  
Sbjct: 461 IFERLMKFTSDWYAPAPTQQTNNSNNSNNTNNNNTTTSPSSSSSSSSSSTTTAAATTVSS 520

Query: 300 VLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAGGSLSGLPPVIEASAALYH---- 359
             S         +   + +S    + + +    P P  GS S  P V  +S++       
Sbjct: 521 STSTSSSSSTITSSSSSTVSPPPPSSSSSPSPPPPPPLGSNS--PTVASSSSSSVQDNEE 580

Query: 360 --------------------LLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLED 419
                               LL   D  L+ HL  L +EPQ + LRW+R+LFGREF  +D
Sbjct: 581 EELSSKCNDVVLKCKYIHSILLKQKDFELYQHLDSLDIEPQIYLLRWIRLLFGREFHFDD 640

Query: 420 LLTIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENA 468
           +L IWD +FA   +                        I    +SML Y+R  LL ++ +
Sbjct: 641 VLNIWDALFAYGENLI---------------------LIDYFCISMLTYIREHLLKSD-S 685

BLAST of Carg24197-RA vs. ExPASy Swiss-Prot
Match: Q92609 (TBC1 domain family member 5 OS=Homo sapiens OX=9606 GN=TBC1D5 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 6.8e-26
Identity = 169/685 (24.67%), Postives = 274/685 (40.00%), Query Frame = 0

Query: 65  RGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDN 124
           R + W++ L VLP      I  +  +    R  Y+ ++  H+ +P   K        ++N
Sbjct: 87  RSICWKLFLCVLPQDKSQWISRIEEL----RAWYSNIKEIHITNPR--KVVGQQDLMINN 146

Query: 125 PLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQ 184
           PLSQ+  SLW +FF+  EL  M++QD+ R +PE   +FQ    + +L  +L  +  ++ Q
Sbjct: 147 PLSQDEGSLWNKFFQDKELRSMIEQDVKRTFPEM-QFFQQENVRKILTDVLFCYARENEQ 206

Query: 185 FGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCL 244
             Y+QGMHELLAP ++VLH D + F                                   
Sbjct: 207 LLYKQGMHELLAPIVFVLHCDHQAF----------------------------------- 266

Query: 245 DTTEDELGVHGNEENVKGLSELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFD 304
                   +H +E                     A  +E E+  VL+  ++EHDAY +F 
Sbjct: 267 --------LHASE--------------------SAQPSE-EMKTVLNPEYLEHDAYAVFS 326

Query: 305 ALMSGAH---------GAVAMADFFSPMPAGGSLSGLPP---VIEASAALYHLLSHVDSS 364
            LM  A          G        +P+P        P    V + +    HLL   D  
Sbjct: 327 QLMETAEPWFSTFEHDGQKGKETLMTPIPFARPQDLGPTIAIVTKVNQIQDHLLKKHDIE 386

Query: 365 LHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLLTIWDEIFASDNSKFDRSDEPETSSSF 424
           L+ HL  L + PQ + LRW+R+LFGREF L+DLL +WD +FA   S              
Sbjct: 387 LYMHLNRLEIAPQIYGLRWVRLLFGREFPLQDLLVVWDALFADGLSL------------- 446

Query: 425 SFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKDVDLSKLIEKA----- 484
                     +  I V+MLLY+R +L+++ N   CL  L+++P   D+  LI KA     
Sbjct: 447 --------GLVDYIFVAMLLYIRDALISS-NYQTCLGLLMHYPFIGDVHSLILKALFLRD 506

Query: 485 -------------------KSWQTFAM---RSNISSTPL----LSGAYHHHSKSLVA--- 544
                              K+     M   R+N    PL    +S +  +  + L++   
Sbjct: 507 PKRNPRPVTYQFHPNLDYYKARGADLMNKSRTNAKGAPLNINKVSNSLINFGRKLISPAM 566

Query: 545 ----------RGNGRSSGSVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQK 604
                      GN  SS SV   T  +    S+      +  ++Q+ +   S +      
Sbjct: 567 APGSAGGPVPGGNSSSSSSVVIPTRTSAEAPSH-----HLQQQQQQQRLMKSESMPVQLN 626

Query: 605 KGWSEKVRFLYRTESDPSPAKLAGGKKNTKS-----SVRRRLLADLARELGAEEDIEKCG 664
           KG S K        S PS   L GG++ T S     + +    ++++R     + + +  
Sbjct: 627 KGLSSK-----NISSSPSVESLPGGREFTGSPPSSATKKDSFFSNISRSRSHSKTMGRKE 666

Query: 665 SDEVLDKKDDISIEGEVDGHDGCEKYSEDAEDKRLES-GIVGSEENSSVFSDPTSSFSGA 688
           S+E L+ +    ++G+++  D   KY     D  L +   V  +EN         S +G 
Sbjct: 687 SEEELEAQISF-LQGQLNDLDAMCKYCAKVMDTHLVNIQDVILQENLEKEDQILVSLAGL 666

BLAST of Carg24197-RA vs. ExPASy Swiss-Prot
Match: Q9NVG8 (TBC1 domain family member 13 OS=Homo sapiens OX=9606 GN=TBC1D13 PE=1 SV=3)

HSP 1 Score: 76.3 bits (186), Expect = 1.9e-12
Identity = 106/425 (24.94%), Postives = 175/425 (41.18%), Query Frame = 0

Query: 64  LRGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKD--GSNSPDT 123
           LR + W+I L  LP    +       + A  R  YA   R  ++ P I+K   G +  D 
Sbjct: 40  LRCLCWKILLNYLPLERAS----WTSILAKQRELYAQFLREMIIQPGIAKANMGVSREDV 99

Query: 124 M--DNPLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWC 183
              D+PL+ NPDS W  +F+  E+   +D+D+ RL P+  S+FQ                
Sbjct: 100 TFEDHPLNPNPDSRWNTYFKDNEVLLQIDKDVRRLCPDI-SFFQ---------------- 159

Query: 184 LQHPQFGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFD 243
                            P L +L    E F  +RK  E            + G    +  
Sbjct: 160 ------------RATDYPCLLILDPQNE-FETLRKRVEQTTLKSQTVARNRSGVTNMSSP 219

Query: 244 FKNCLDTTEDEL-----GVHGNEENVKGL----SELDPEIQTIILLTDAYG-AEGELGIV 303
            KN + ++ +E      G   + E V+ +    ++L+P I  +  + +  G         
Sbjct: 220 HKNSVPSSLNEYEVLPNGCEAHWEVVERILFIYAKLNPGIAYVQGMNEIVGPLYYTFATD 279

Query: 304 LSERFIEH---DAYTMFDALMSGAHGAVAMADFFSPMPAGGSLSGLPPVIEASAALYHLL 363
            +  + EH   D +  F  LM     A    +F   +    S  G+   +E    +Y  L
Sbjct: 280 PNSEWKEHAEADTFFCFTNLM-----AEIRDNFIKSL--DDSQCGITYKME---KVYSTL 339

Query: 364 SHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLLTIWDEIFASDNSKFDRSDEP 423
              D  L+  L E  ++PQ+F  RWL +L  +EFLL D++ IWD +FA DN +FD     
Sbjct: 340 KDKDVELYLKLQEQNIKPQFFAFRWLTLLLSQEFLLPDVIRIWDSLFADDN-RFD----- 397

Query: 424 ETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKDVDLSKLIEK 472
                          F+  +  +ML+ +R  LL  +  T+ ++ L ++P   D+ ++++K
Sbjct: 400 ---------------FLLLVCCAMLMLIREQLLEGD-FTVNMRLLQDYP-ITDVCQILQK 397

BLAST of Carg24197-RA vs. ExPASy TrEMBL
Match: A0A6J1FC80 (TBC1 domain family member 5-like OS=Cucurbita moschata OX=3662 GN=LOC111444080 PE=4 SV=1)

HSP 1 Score: 1493.8 bits (3866), Expect = 0.0e+00
Identity = 765/775 (98.71%), Postives = 768/775 (99.10%), Query Frame = 0

Query: 27  MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 86
           MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH
Sbjct: 1   MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 60

Query: 87  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 146
           LRRVTADSRRRYAILRRRHLVDPHISK+GSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM
Sbjct: 61  LRRVTADSRRRYAILRRRHLVDPHISKEGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 120

Query: 147 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 206
           VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV
Sbjct: 121 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 180

Query: 207 ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL 266
           ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL
Sbjct: 181 ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL 240

Query: 267 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 326
           DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMF+ALMSGAHGAVAMADFFSPMPAG
Sbjct: 241 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFEALMSGAHGAVAMADFFSPMPAG 300

Query: 327 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 386
           GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL
Sbjct: 301 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 360

Query: 387 TIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 446
           TIWDEIFASDNSKFDRSDEPETSSSF FLSSSRGAFIAAIAVSMLLYLRSSLLATENATL
Sbjct: 361 TIWDEIFASDNSKFDRSDEPETSSSFDFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 420

Query: 447 CLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 506
           CLQRLLNFPKDVDL KLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGN RSSG
Sbjct: 421 CLQRLLNFPKDVDLPKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNDRSSG 480

Query: 507 SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 566
           SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVR LYRTESDPS
Sbjct: 481 SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRSLYRTESDPS 540

Query: 567 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC 626
           PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC
Sbjct: 541 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC 600

Query: 627 EKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSPDEN 686
           EKYSE+AED RLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLS DEN
Sbjct: 601 EKYSENAEDNRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSLDEN 660

Query: 687 DDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 746
           DDQSQSVVEGSPVPVPDQLENI EKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS
Sbjct: 661 DDQSQSVVEGSPVPVPDQLENIQEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 720

Query: 747 SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 802
           SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG
Sbjct: 721 SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 775

BLAST of Carg24197-RA vs. ExPASy TrEMBL
Match: A0A6J1HSK8 (TBC1 domain family member 5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465778 PE=4 SV=1)

HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 756/775 (97.55%), Postives = 763/775 (98.45%), Query Frame = 0

Query: 27  MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 86
           MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSS ASIDH
Sbjct: 1   MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSSASIDH 60

Query: 87  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 146
           LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM
Sbjct: 61  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 120

Query: 147 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 206
           VDQDLSRLYPEHGSYFQT GCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV
Sbjct: 121 VDQDLSRLYPEHGSYFQTSGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 180

Query: 207 ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL 266
            RFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLD T+DELGVHGNEENVKGLSEL
Sbjct: 181 VRFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDMTDDELGVHGNEENVKGLSEL 240

Query: 267 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 326
           DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG
Sbjct: 241 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 300

Query: 327 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 386
           GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL
Sbjct: 301 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 360

Query: 387 TIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 446
           TIWDEIFASDNSKFDRSDEPETSSSF FLSSSRGAFIAAIAVSMLLYLRSSLLATENATL
Sbjct: 361 TIWDEIFASDNSKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 420

Query: 447 CLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 506
           CLQRLLNFPKDVDL KLIEKAKSWQT AMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG
Sbjct: 421 CLQRLLNFPKDVDLPKLIEKAKSWQTLAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 480

Query: 507 SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 566
           SVSPKTPLN VPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS
Sbjct: 481 SVSPKTPLNHVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 540

Query: 567 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC 626
           PAKLAGGKKNTKSSVRRRLLADLARELGAEED++KC S+EVLDKKDDISIEGEVDGHDGC
Sbjct: 541 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDLKKCESNEVLDKKDDISIEGEVDGHDGC 600

Query: 627 EKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSPDEN 686
           EKYSE+AEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLS DEN
Sbjct: 601 EKYSENAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSLDEN 660

Query: 687 DDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 746
           DDQSQSVVEGSP+PVPDQLENI EKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS
Sbjct: 661 DDQSQSVVEGSPLPVPDQLENIPEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 720

Query: 747 SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 802
           SEGKGGVE+SKSTGVESNPIKNI SPKTDGACSTSGSGKGDGVDQNMMGTLKNLG
Sbjct: 721 SEGKGGVESSKSTGVESNPIKNIASPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 775

BLAST of Carg24197-RA vs. ExPASy TrEMBL
Match: A0A6J1HQI6 (TBC1 domain family member 5-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465778 PE=4 SV=1)

HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 756/775 (97.55%), Postives = 763/775 (98.45%), Query Frame = 0

Query: 27  MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 86
           MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSS ASIDH
Sbjct: 1   MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSSASIDH 60

Query: 87  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 146
           LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM
Sbjct: 61  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQNPDSLWGRFFRSAELEKM 120

Query: 147 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 206
           VDQDLSRLYPEHGSYFQT GCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV
Sbjct: 121 VDQDLSRLYPEHGSYFQTSGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDV 180

Query: 207 ERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSEL 266
            RFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLD T+DELGVHGNEENVKGLSEL
Sbjct: 181 VRFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDMTDDELGVHGNEENVKGLSEL 240

Query: 267 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 326
           DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG
Sbjct: 241 DPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAG 300

Query: 327 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 386
           GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL
Sbjct: 301 GSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLL 360

Query: 387 TIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 446
           TIWDEIFASDNSKFDRSDEPETSSSF FLSSSRGAFIAAIAVSMLLYLRSSLLATENATL
Sbjct: 361 TIWDEIFASDNSKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATENATL 420

Query: 447 CLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 506
           CLQRLLNFPKDVDL KLIEKAKSWQT AMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG
Sbjct: 421 CLQRLLNFPKDVDLPKLIEKAKSWQTLAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSG 480

Query: 507 SVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 566
           SVSPKTPLN VPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS
Sbjct: 481 SVSPKTPLNHVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS 540

Query: 567 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGC 626
           PAKLAGGKKNTKSSVRRRLLADLARELGAEED++KC S+EVLDKKDDISIEGEVDGHDGC
Sbjct: 541 PAKLAGGKKNTKSSVRRRLLADLARELGAEEDLKKCESNEVLDKKDDISIEGEVDGHDGC 600

Query: 627 EKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSPDEN 686
           EKYSE+AEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLS DEN
Sbjct: 601 EKYSENAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSLDEN 660

Query: 687 DDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 746
           DDQSQSVVEGSP+PVPDQLENI EKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS
Sbjct: 661 DDQSQSVVEGSPLPVPDQLENIPEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVRNAS 720

Query: 747 SEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 802
           SEGKGGVE+SKSTGVESNPIKNI SPKTDGACSTSGSGKGDGVDQNMMGTLKNLG
Sbjct: 721 SEGKGGVESSKSTGVESNPIKNIASPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 775

BLAST of Carg24197-RA vs. ExPASy TrEMBL
Match: A0A0A0LHC4 (Rab-GAP TBC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G061030 PE=4 SV=1)

HSP 1 Score: 1326.2 bits (3431), Expect = 0.0e+00
Identity = 685/778 (88.05%), Postives = 717/778 (92.16%), Query Frame = 0

Query: 27  MAPSEIVAALSEPASRT--PSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASI 86
           MAPSEIV ALSEP S T   SCSGSV+HR SEDKR FVDLRGVRWRINLGVLPSSSLASI
Sbjct: 1   MAPSEIVPALSEPTSTTSSSSCSGSVFHRISEDKREFVDLRGVRWRINLGVLPSSSLASI 60

Query: 87  DHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSPD-TMDNPLSQNPDSLWGRFFRSAEL 146
           D LRRVTADSRRRYAILRRRHLVDPH+SKD S+SPD  MDNPLSQNPDS+WGRFFRSAEL
Sbjct: 61  DDLRRVTADSRRRYAILRRRHLVDPHVSKDESSSPDIAMDNPLSQNPDSMWGRFFRSAEL 120

Query: 147 EKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLH 206
           EKMVDQDLSRLYPEHGSYFQTPGCQ LLRRILLLWCLQHPQFGYRQGMHELLAP LYVLH
Sbjct: 121 EKMVDQDLSRLYPEHGSYFQTPGCQSLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180

Query: 207 VDVERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGL 266
           VDVER SQVRKLYEDQFADKFDG+SFQDGSFKYNFDFKN LD+TEDE GV GN E+VK L
Sbjct: 181 VDVERLSQVRKLYEDQFADKFDGLSFQDGSFKYNFDFKNRLDSTEDEFGVDGNVESVKSL 240

Query: 267 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPM 326
           SELDPEIQTIILLTDAYGAEGELGIVLS+RFIEHDAYTMFDALMSGAHG VAMADF+S  
Sbjct: 241 SELDPEIQTIILLTDAYGAEGELGIVLSDRFIEHDAYTMFDALMSGAHGEVAMADFYSST 300

Query: 327 PAGGSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLE 386
           PAGGSLSGLPPVIEAS+ALYHLLSHVDSSLHAHLVELGVEPQYF LRWLRVLFGREF LE
Sbjct: 301 PAGGSLSGLPPVIEASSALYHLLSHVDSSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360

Query: 387 DLLTIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 446
           DLLTIWDEIFASDNSKFDRSDEPETSSSF FLSSSRGAFIAAIAVSMLLYLRSSLLATEN
Sbjct: 361 DLLTIWDEIFASDNSKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420

Query: 447 ATLCLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGR 506
           ATLCLQRLLNFPK+VDL KLIEKAKS QT AM SNISS+PLLSGAYHHHSKS+VARGNGR
Sbjct: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLAMHSNISSSPLLSGAYHHHSKSIVARGNGR 480

Query: 507 SSGSVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTES 566
           SSGSVSPKTPLN VPESYWEEKWRVLH+EQE KQSGSR  NAAQKKGWSEKVRFLYRTES
Sbjct: 481 SSGSVSPKTPLNHVPESYWEEKWRVLHKEQEFKQSGSRGNNAAQKKGWSEKVRFLYRTES 540

Query: 567 DPSPAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGH 626
           DP P KL GGKKNTKSSVRRRLLADL+RELGAEED EKCG+DEV++ KDD+S+EGEVDG 
Sbjct: 541 DPFPGKLVGGKKNTKSSVRRRLLADLSRELGAEEDSEKCGNDEVVNNKDDLSVEGEVDGQ 600

Query: 627 DGCEKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSP 686
           DGCEKY E+AEDKR ESGI GSEENSS+FSDPTSSFSGANDNE D+NDSSRSSVAS+LS 
Sbjct: 601 DGCEKYLENAEDKRCESGIAGSEENSSIFSDPTSSFSGANDNEPDLNDSSRSSVASNLSL 660

Query: 687 DENDDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVR 746
           DENDDQSQS+VEGS +PVPDQLENI EKSGC NDSEGN +VG +++KLLGKFPWFWKF R
Sbjct: 661 DENDDQSQSIVEGSSLPVPDQLENIPEKSGCTNDSEGNAAVGAKERKLLGKFPWFWKFGR 720

Query: 747 NASSEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 802
           NA SEGKG  EASK  G E+NPIKNI  PK DGACSTS SGKGDGVDQNMMGTLKN+G
Sbjct: 721 NAVSEGKGDTEASKLAGAENNPIKNIAPPKIDGACSTSVSGKGDGVDQNMMGTLKNIG 778

BLAST of Carg24197-RA vs. ExPASy TrEMBL
Match: A0A5A7UD37 (RabGAP-TBC domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold751G00030 PE=4 SV=1)

HSP 1 Score: 1317.0 bits (3407), Expect = 0.0e+00
Identity = 676/778 (86.89%), Postives = 719/778 (92.42%), Query Frame = 0

Query: 27  MAPSEIVAALSEPASRT--PSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASI 86
           MAPSEIV ALSEP S T   SCSGSV+HR SEDKR+FVDLRGVRWRINLGVLPSSSLASI
Sbjct: 1   MAPSEIVPALSEPTSTTSSSSCSGSVFHRISEDKRQFVDLRGVRWRINLGVLPSSSLASI 60

Query: 87  DHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSPD-TMDNPLSQNPDSLWGRFFRSAEL 146
           D LRRVTADSRRRYAILRRRHLVDPH+SKDGS+SPD  MDNPLSQNPDS+WGRFFRSAEL
Sbjct: 61  DDLRRVTADSRRRYAILRRRHLVDPHVSKDGSSSPDIAMDNPLSQNPDSMWGRFFRSAEL 120

Query: 147 EKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLH 206
           EKMVDQDLSRLYPEHGSYFQTPGCQ LLRRILLLWCLQHPQFGYRQGMHELLAP LYVLH
Sbjct: 121 EKMVDQDLSRLYPEHGSYFQTPGCQSLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180

Query: 207 VDVERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGL 266
           VDVER SQVRKLYEDQFADKFDG+SFQDGSFKYNFDFKN LD+TEDE G  GN E+VK L
Sbjct: 181 VDVERLSQVRKLYEDQFADKFDGLSFQDGSFKYNFDFKNRLDSTEDEFGADGNVESVKSL 240

Query: 267 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPM 326
           SELDPEIQTIILLTDAYGAEGELGIVLS+RFIEHDAYTMFDALMSGAHG VAMADF+SP 
Sbjct: 241 SELDPEIQTIILLTDAYGAEGELGIVLSDRFIEHDAYTMFDALMSGAHGEVAMADFYSPT 300

Query: 327 PAGGSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLE 386
           PAGGSLSGLPPVIEAS+ALYHLLSHVDSSLHAHLVELGVEPQYF LRWLRVLFGREF LE
Sbjct: 301 PAGGSLSGLPPVIEASSALYHLLSHVDSSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360

Query: 387 DLLTIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 446
           DLLTIWDEIFASDN+KFDRSDEPETSSSF FLSSSRGAFIAAIAVSMLLYLRSSLLATEN
Sbjct: 361 DLLTIWDEIFASDNTKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420

Query: 447 ATLCLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGR 506
           ATLCLQRLLNFPK+VDL KLIEKAKS QT AM SNISS+PLLSGAYHHHSKS+V RGNG 
Sbjct: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLAMHSNISSSPLLSGAYHHHSKSIVGRGNGL 480

Query: 507 SSGSVSPKTPLNQVPESYWEEKWRVLHREQECKQSGSRTQNAAQKKGWSEKVRFLYRTES 566
           SSGSVSPKTPLN VPESYWEEKWRVLH+EQE KQSGS+++NAAQKKGWSEKVRFLYRTES
Sbjct: 481 SSGSVSPKTPLNHVPESYWEEKWRVLHKEQEFKQSGSKSKNAAQKKGWSEKVRFLYRTES 540

Query: 567 DPSPAKLAGGKKNTKSSVRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGH 626
           DP PAKL GGKKNTKSSVRRRLLADL+RELGAEED EKCG+DE+++ KDD+S+EGEVDG 
Sbjct: 541 DPFPAKLVGGKKNTKSSVRRRLLADLSRELGAEEDSEKCGNDEIVNNKDDLSVEGEVDGQ 600

Query: 627 DGCEKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSP 686
           DGCEKY E+AEDKR  SGI GSEENSS+FSDPTSSFSGANDNE D+NDS+RSSVAS+LS 
Sbjct: 601 DGCEKYLENAEDKRCGSGIAGSEENSSIFSDPTSSFSGANDNEPDLNDSNRSSVASNLSL 660

Query: 687 DENDDQSQSVVEGSPVPVPDQLENIHEKSGCNNDSEGNVSVGTRDKKLLGKFPWFWKFVR 746
           DENDDQSQS+VEGS +PVPDQLENI EKSGC ND EGN +VG +++KLLGKFPWFWKF R
Sbjct: 661 DENDDQSQSIVEGSSLPVPDQLENIPEKSGCTNDCEGNPAVGAKERKLLGKFPWFWKFGR 720

Query: 747 NASSEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMGTLKNLG 802
           NA+SEGKG  EASK  G E+NPIK+I  P+ DGACSTS SGKGDGVDQN+MGTLKN+G
Sbjct: 721 NAASEGKGDTEASKLAGAENNPIKHIAPPRIDGACSTSISGKGDGVDQNVMGTLKNIG 778

BLAST of Carg24197-RA vs. TAIR 10
Match: AT4G29950.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 700.7 bits (1807), Expect = 1.5e-201
Identity = 425/786 (54.07%), Postives = 529/786 (67.30%), Query Frame = 0

Query: 56  EDKRRFVDLRGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDG 115
           E  RRF +LRG+RWR+NLGVLP  S +SID LR+ TA+SRRRYA LRRR L+DPH+SKD 
Sbjct: 12  ESDRRFANLRGLRWRVNLGVLPFQS-SSIDDLRKATAESRRRYAALRRRLLIDPHLSKDV 71

Query: 116 SNSPD-TMDNPLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRI 175
            NSPD ++DNPLSQNPDS WGRFFR+AELEK +DQDLSRLYPEH SYFQ PGCQG+LRRI
Sbjct: 72  RNSPDLSIDNPLSQNPDSTWGRFFRNAELEKTLDQDLSRLYPEHWSYFQAPGCQGMLRRI 131

Query: 176 LLLWCLQHPQFGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADKFDGVSFQDGSF 235
           LLLWCL+HP++GYRQGMHELLAP LYVLHVDV+R S+VRK YED F D+FDG+SF++   
Sbjct: 132 LLLWCLKHPEYGYRQGMHELLAPLLYVLHVDVDRLSEVRKSYEDHFIDRFDGLSFEERDI 191

Query: 236 KYNFDFKNCL-DTTEDEL-GVHGNEENVKGLSELDPEIQTIILLTDAYGAEGELGIVLSE 295
            YNF+FK  L D T+DE+ G+ GN + +K L ELDPEIQ+I+ L+DAYGAEGELGIVLSE
Sbjct: 192 TYNFEFKKFLEDFTDDEIGGIQGNSKKIKSLDELDPEIQSIVRLSDAYGAEGELGIVLSE 251

Query: 296 RFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAGGSLSGLPPVIEASAALYHLLSHVDSS 355
           +F+EHDAY MFDALM+G HG VAMA FF+  PA GS +GLPPV+EAS A YHLLS VDSS
Sbjct: 252 KFMEHDAYCMFDALMNGVHGCVAMAGFFAYSPASGSHTGLPPVLEASTAFYHLLSFVDSS 311

Query: 356 LHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLLTIWDEIFASDNSKFDRSDEPETSSSF 415
           LH+HLVELGVEPQYF LRWLRVLFGREFLL+DLL +WDEIF++DN+    +D   T+ S+
Sbjct: 312 LHSHLVELGVEPQYFGLRWLRVLFGREFLLQDLLIVWDEIFSADNTTRTDADN-NTNQSY 371

Query: 416 SFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKDVDLSKLIEKAKSWQT 475
               S RGA I+ +AVSM+L LRSSLLATENA  CLQRLLNFP+ +D+ K+IEKAKS QT
Sbjct: 372 KIFDSPRGALISGMAVSMILCLRSSLLATENAASCLQRLLNFPEKIDVRKIIEKAKSLQT 431

Query: 476 FAMRSNISSTPLL--SGAYHHHSKSLVARGNGRSSGSVSPKTPLNQVPESYWEEKWRVLH 535
            A+  ++ S+ L    G     S ++ AR N   SGS SPK+PL   P+SYWE++WRVLH
Sbjct: 432 LALDDDVRSSALSINDGFDQSISPAVPARTNSFPSGSTSPKSPLIITPQSYWEDQWRVLH 491

Query: 536 R-EQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS-PAKLAGGKKNTK-SSVRRRLLA 595
           +  +E K+S S  Q   +KK W  +V+ L+R ES+P+  AK   GK   K SSV R LL 
Sbjct: 492 KAAEEEKKSPSPIQ---KKKPWF-RVKRLFRAESEPTHSAKSPNGKSEVKISSVARNLLE 551

Query: 596 DLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGCEKYSEDAEDKRLESGIVGSEE 655
           D  R+L   E +E    D V    +D SI    D +   E  +E++        IV  E 
Sbjct: 552 DFNRQL-VSEPVEANPIDVV--NNEDSSIRETEDINTDFETAAEES--------IVMEEN 611

Query: 656 NSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSPDE---------------------- 715
           +S +FSDP S    +N  END    S SS  S+L PDE                      
Sbjct: 612 SSDLFSDPNSPLRDSNYIEND----SDSSNESNLFPDETVKDRETSVVDSPLSISSQPSM 671

Query: 716 ------NDDQSQSVVEGSPVPVPDQLE---NIHEKSGCNNDSEGNV-SVGTRDKKLLGKF 775
                 + DQ  SVV+ SP+PV  Q      + + +  +N ++ +V ++  R K L GKF
Sbjct: 672 EFIVSLSKDQETSVVD-SPLPVSSQPSIEFPVTQSNDEDNAADKSVANIKERSKVLPGKF 731

Query: 776 PWFWKFVRNASSEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVDQNMMG 802
            WFWKF RN ++      E ++  GVES+    + S ++      S SG     DQN+M 
Sbjct: 732 QWFWKFGRNVTA------EETRCNGVESSKSDLVCSSESHSLPQASSSGSKGDTDQNVMN 769

BLAST of Carg24197-RA vs. TAIR 10
Match: AT5G57210.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 605.5 bits (1560), Expect = 6.4e-173
Identity = 343/590 (58.14%), Postives = 423/590 (71.69%), Query Frame = 0

Query: 60  RFVDLRGVRWRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSP 119
           RF  LRGVRWRINLG+LPSS  ++ID LRRVTADSRRRYA LRRR L+DPH+ K G+NSP
Sbjct: 29  RFEKLRGVRWRINLGILPSSPSSTIDELRRVTADSRRRYAALRRRLLIDPHLPKKGTNSP 88

Query: 120 D-TMDNPLSQNPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLW 179
           D T+DNPLSQNPDS WGRFFR+AELEK +DQDLSRLYPEHGSYFQ+ GCQG+LRRILLLW
Sbjct: 89  DLTIDNPLSQNPDSTWGRFFRNAELEKTLDQDLSRLYPEHGSYFQSSGCQGMLRRILLLW 148

Query: 180 CLQHPQFGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADKFDGVSFQD-GSFKYN 239
           CL+HP+ GYRQGMHELLAP LYVL VDV+  ++VR  YEDQF D FD ++FQ+  S  Y+
Sbjct: 149 CLKHPEIGYRQGMHELLAPLLYVLQVDVQYLTEVRSNYEDQFVDLFDELAFQERDSGAYD 208

Query: 240 FDFKNCLDTTEDELGVHG------NEENVKGLSELDPEIQTIILLTDAYGAEGELGIVLS 299
           FD K  LD + ++    G       ++  K   ELD E QT +LL+DAYG EGELGIVLS
Sbjct: 209 FDIKKVLDDSMEDEEEDGPPSGSTKKKKPKSFDELDTETQTAVLLSDAYGGEGELGIVLS 268

Query: 300 ERFIEHDAYTMFDALMSGAH--GAVAMADFFSPMPAGGSLSGLPPVIEASAALYHLLSHV 359
           ++F+EHDAYTMFDALM G    G+V++A+FF       S++GLPPVIEAS ALYHLLS V
Sbjct: 269 DKFMEHDAYTMFDALMYGGSSLGSVSVANFFIYSAPNDSITGLPPVIEASGALYHLLSLV 328

Query: 360 DSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLLTIWDEIFASDNSKFDRSDEPETS 419
           D+SLH+HLVELGVEPQYF LRWLRVLFGREF L +LL +WDEIF++DNS+ +R  E +  
Sbjct: 329 DASLHSHLVELGVEPQYFALRWLRVLFGREFPLSNLLIVWDEIFSADNSEVERGVEADLG 388

Query: 420 SSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKDVDLSKLIEKAKS 479
             F  LSS RGA +A +AVSM+LYLRSSLLATENAT  L++LLNFP+D+DLSK+IEKAK+
Sbjct: 389 CEFRILSSPRGALVAGMAVSMILYLRSSLLATENATSSLKKLLNFPEDIDLSKVIEKAKT 448

Query: 480 WQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGRSSGSVS-PKTPLNQVPESYWEEKWRV 539
            Q+ A+  N     +  G           RG+  S  S+S   +P+   PESYWEEKWRV
Sbjct: 449 LQSLALEINARRDLIPKG------PRKPMRGHSLSVDSISLGSSPVGIEPESYWEEKWRV 508

Query: 540 LH-REQECKQSGSRTQNAAQKKGWSEKVRF-LYRTESDPSPAKLAGGKKNTKSSVRRRLL 599
           L+  E+E ++  +  +  A KK WSE+V+  L RTESDPSPA+    +   K  +RR LL
Sbjct: 509 LNSAEEEERRKKALQRPKAGKKSWSERVKLRLSRTESDPSPAE--AKRSGNKPHIRRSLL 568

Query: 600 ADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGCEKYSEDAEDK 637
            DL+R+LG E++IE     E  +   DI     V      E Y +++ DK
Sbjct: 569 DDLSRQLG-EKEIE---PPEFPNPDTDIERSSTVSDTPSAE-YEDNSSDK 605

BLAST of Carg24197-RA vs. TAIR 10
Match: AT2G19240.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 599.4 bits (1544), Expect = 4.6e-171
Identity = 387/825 (46.91%), Postives = 499/825 (60.48%), Query Frame = 0

Query: 27  MAPSEIVAALSEPASRTPSCSGSVYHRDSEDKRRFVDLRGVRWRINLGVLPSSSLASIDH 86
           MAPSEI  AL EP       SGS+    S + RRF +LRGVRWR+NLGVLPS + +SID 
Sbjct: 1   MAPSEIEPALQEP-------SGSLVL--SREDRRFANLRGVRWRVNLGVLPSLA-SSIDE 60

Query: 87  LRRVTADSRRRYAILRRRHLVDPHISKDGSNSPD-TMDNPLSQNPDSLWGRFFRSAELEK 146
            RR  A+SRR     RRR L+DPH+ K   +SP+  +DNPLSQNP+S WG+FFR+AELEK
Sbjct: 61  FRRAAANSRR-----RRRLLMDPHVLKHEDSSPNFIIDNPLSQNPNSTWGQFFRNAELEK 120

Query: 147 MVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVD 206
            +DQDLSRLYPEH  YFQTP  QG+LRRILLLWCL+HP++GYRQGMHELLAP LYVLHVD
Sbjct: 121 TLDQDLSRLYPEHWCYFQTPRYQGMLRRILLLWCLKHPEYGYRQGMHELLAPLLYVLHVD 180

Query: 207 VERFSQVRKLYEDQFADKFDGVSFQDGSFKYNFDFKNCLDTTEDELGVHGNEENVKGLSE 266
           + R S+VRK YED F D+FD +SF +    Y FDF   +D+ ++ +G  G+ +N   L E
Sbjct: 181 IMRLSEVRKSYEDYFTDRFDSLSFMERDITYTFDFNKFMDSVDNGIGSQGHSKNFNSLDE 240

Query: 267 LDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPA 326
           LDPE+Q++++LTD+YG E ELGIVLSE+F+EHDAY MFDALMSG HG  AMA FFS  PA
Sbjct: 241 LDPEVQSLVMLTDSYGTESELGIVLSEKFMEHDAYCMFDALMSGIHGCFAMASFFSYSPA 300

Query: 327 GGSLSGLPPVIEASAALYHLLSHVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDL 386
            GS +GL PV+EA +A Y +L+ VDSSLH+HLVELGVEPQYF LRWLRVLFGREFLL+DL
Sbjct: 301 SGSHTGLTPVLEACSAFYRILAVVDSSLHSHLVELGVEPQYFGLRWLRVLFGREFLLQDL 360

Query: 387 LTIWDEIFASDNSKFDRSDEPETSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENAT 446
           L +WDEI  +DNS   R+DE   + +F      RG  +  + VSM+LYLRSSLL+TENAT
Sbjct: 361 LLVWDEIILADNSA--RTDEDSRNQNFRIFDCPRGTLVLGMTVSMILYLRSSLLSTENAT 420

Query: 447 LCLQRLLNFPKDVDLSKLIEKAKSWQTFAMRSNISSTPLLSGAYHHHSKSLVARGNGRSS 506
            CLQRLLNFP+++DL+K+I+KAK  Q   + +++ S   ++G +   S  + AR     S
Sbjct: 421 CCLQRLLNFPENIDLNKIIQKAKLLQALVLDTDMLSALSINGVF-DQSNFVPAR---TKS 480

Query: 507 GSVSPKTPLNQVPESYWEEKWRVLHREQECKQS---GSRTQNAAQKKGWSEKVRFLYRTE 566
            S SP++PL   PESYWE+KWRVLH+ +E +       +T    +KK W   V  L+R  
Sbjct: 481 CSTSPRSPLIIAPESYWEKKWRVLHKAEEEENKICLEKQTPPTQKKKRWL-NVTKLFRAV 540

Query: 567 SDPSPAKLAGGKKNTKSS-VRRRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVD 626
            D S  KL  G++   SS V + LL D + +L  +     C    V   K++I  +   +
Sbjct: 541 IDLSHHKLGIGERKANSSPVTQSLLEDSSEQLNVD-----C---HVTVNKENIHPQ---E 600

Query: 627 GHDGCEKYSEDAEDKRLESGIVGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSL 686
             +   ++   A+++ + SG   SEE+S V  DPTS    +   END    S SS  S+L
Sbjct: 601 TEENIMEF-HSADEESIVSGSSPSEESSFVSLDPTSPVRCSTKIEND----SVSSAGSNL 660

Query: 687 SPDEND---------------------------------------DQSQSVVEGSPVPVP 746
            PDE+D                                       D+ Q     SP+ V 
Sbjct: 661 LPDEDDKSIVSISEENSSVSSDPISPAIDSNYSGKYLDCCTGSENDKDQQTSVNSPLSVS 720

Query: 747 DQLENIHEKSGCNNDSEGNVSVG-TRDKKLL-GKFPWFWKFVRNASSEGKGGVEASKSTG 802
               N +  +  + D   + SVG T++ KLL G   WF K  R  SSE     +AS +T 
Sbjct: 721 PHRRNEYPVTQSDEDVSTDKSVGITKEYKLLHGIVQWFRKLKRTLSSEETSHRKASDATK 780

BLAST of Carg24197-RA vs. TAIR 10
Match: AT4G29950.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 526.2 bits (1354), Expect = 5.0e-149
Identity = 339/671 (50.52%), Postives = 431/671 (64.23%), Query Frame = 0

Query: 170 LLRRILLLWCLQHPQFGYRQGMHELLAPFLYVLHVDVERFSQVRKLYEDQFADKFDGVSF 229
           +LRRILLLWCL+HP++GYRQGMHELLAP LYVLHVDV+R S+VRK YED F D+FDG+SF
Sbjct: 1   MLRRILLLWCLKHPEYGYRQGMHELLAPLLYVLHVDVDRLSEVRKSYEDHFIDRFDGLSF 60

Query: 230 QDGSFKYNFDFKNCL-DTTEDEL-GVHGNEENVKGLSELDPEIQTIILLTDAYGAEGELG 289
           ++    YNF+FK  L D T+DE+ G+ GN + +K L ELDPEIQ+I+ L+DAYGAEGELG
Sbjct: 61  EERDITYNFEFKKFLEDFTDDEIGGIQGNSKKIKSLDELDPEIQSIVRLSDAYGAEGELG 120

Query: 290 IVLSERFIEHDAYTMFDALMSGAHGAVAMADFFSPMPAGGSLSGLPPVIEASAALYHLLS 349
           IVLSE+F+EHDAY MFDALM+G HG VAMA FF+  PA GS +GLPPV+EAS A YHLLS
Sbjct: 121 IVLSEKFMEHDAYCMFDALMNGVHGCVAMAGFFAYSPASGSHTGLPPVLEASTAFYHLLS 180

Query: 350 HVDSSLHAHLVELGVEPQYFFLRWLRVLFGREFLLEDLLTIWDEIFASDNSKFDRSDEPE 409
            VDSSLH+HLVELGVEPQYF LRWLRVLFGREFLL+DLL +WDEIF++DN+    +D   
Sbjct: 181 FVDSSLHSHLVELGVEPQYFGLRWLRVLFGREFLLQDLLIVWDEIFSADNTTRTDADN-N 240

Query: 410 TSSSFSFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKDVDLSKLIEKA 469
           T+ S+    S RGA I+ +AVSM+L LRSSLLATENA  CLQRLLNFP+ +D+ K+IEKA
Sbjct: 241 TNQSYKIFDSPRGALISGMAVSMILCLRSSLLATENAASCLQRLLNFPEKIDVRKIIEKA 300

Query: 470 KSWQTFAMRSNISSTPLL--SGAYHHHSKSLVARGNGRSSGSVSPKTPLNQVPESYWEEK 529
           KS QT A+  ++ S+ L    G     S ++ AR N   SGS SPK+PL   P+SYWE++
Sbjct: 301 KSLQTLALDDDVRSSALSINDGFDQSISPAVPARTNSFPSGSTSPKSPLIITPQSYWEDQ 360

Query: 530 WRVLHR-EQECKQSGSRTQNAAQKKGWSEKVRFLYRTESDPS-PAKLAGGKKNTK-SSVR 589
           WRVLH+  +E K+S S  Q   +KK W  +V+ L+R ES+P+  AK   GK   K SSV 
Sbjct: 361 WRVLHKAAEEEKKSPSPIQ---KKKPWF-RVKRLFRAESEPTHSAKSPNGKSEVKISSVA 420

Query: 590 RRLLADLARELGAEEDIEKCGSDEVLDKKDDISIEGEVDGHDGCEKYSEDAEDKRLESGI 649
           R LL D  R+L   E +E    D V    +D SI    D +   E  +E++        I
Sbjct: 421 RNLLEDFNRQL-VSEPVEANPIDVV--NNEDSSIRETEDINTDFETAAEES--------I 480

Query: 650 VGSEENSSVFSDPTSSFSGANDNENDVNDSSRSSVASSLSPDE----------------- 709
           V  E +S +FSDP S    +N  END    S SS  S+L PDE                 
Sbjct: 481 VMEENSSDLFSDPNSPLRDSNYIEND----SDSSNESNLFPDETVKDRETSVVDSPLSIS 540

Query: 710 -----------NDDQSQSVVEGSPVPVPDQLE---NIHEKSGCNNDSEGNV-SVGTRDKK 769
                      + DQ  SVV+ SP+PV  Q      + + +  +N ++ +V ++  R K 
Sbjct: 541 SQPSMEFIVSLSKDQETSVVD-SPLPVSSQPSIEFPVTQSNDEDNAADKSVANIKERSKV 600

Query: 770 LLGKFPWFWKFVRNASSEGKGGVEASKSTGVESNPIKNIGSPKTDGACSTSGSGKGDGVD 802
           L GKF WFWKF RN ++      E ++  GVES+    + S ++      S SG     D
Sbjct: 601 LPGKFQWFWKFGRNVTA------EETRCNGVESSKSDLVCSSESHSLPQASSSGSKGDTD 644

BLAST of Carg24197-RA vs. TAIR 10
Match: AT2G30710.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 3.6e-06
Identity = 40/133 (30.08%), Postives = 59/133 (44.36%), Query Frame = 0

Query: 69  WRINLGVLPSSSLASIDHLRRVTADSRRRYAILRRRHLVDPHISKDGSNSPDTMDNPLSQ 128
           WR+ LG  P               +S RR A+LRR+ L   ++   G       D P S+
Sbjct: 153 WRLLLGYAP--------------PNSDRREAVLRRKRL--EYLESVG----QFYDLPDSE 212

Query: 129 NPDSLWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYR 188
             D           + + +  D  R  P+  S+FQ    Q  L RIL  W ++HP  GY 
Sbjct: 213 RSDD-------EINMLRQIAVDCPRTVPD-VSFFQQEQVQKSLERILYTWAIRHPASGYV 257

Query: 189 QGMHELLAPFLYV 202
           QG+++L+ PFL +
Sbjct: 273 QGINDLVTPFLVI 257

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7020929.10.0e+00100.00TBC1 domain family member 5, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_022937789.10.0e+0098.71TBC1 domain family member 5-like [Cucurbita moschata] >XP_022937791.1 TBC1 domai... [more]
XP_023537563.10.0e+0098.32TBC1 domain family member 5-like [Cucurbita pepo subsp. pepo][more]
XP_022966053.10.0e+0097.55TBC1 domain family member 5-like isoform X1 [Cucurbita maxima] >XP_022966054.1 T... [more]
XP_022966055.10.0e+0097.55TBC1 domain family member 5-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q80XQ21.7e-3227.27TBC1 domain family member 5 OS=Mus musculus OX=10090 GN=Tbc1d5 PE=1 SV=2[more]
Q54VM37.7e-3026.13TBC1 domain family member 5 homolog A OS=Dictyostelium discoideum OX=44689 GN=tb... [more]
Q54TA51.1e-2825.96TBC1 domain family member 5 homolog B OS=Dictyostelium discoideum OX=44689 GN=tb... [more]
Q926096.8e-2624.67TBC1 domain family member 5 OS=Homo sapiens OX=9606 GN=TBC1D5 PE=1 SV=1[more]
Q9NVG81.9e-1224.94TBC1 domain family member 13 OS=Homo sapiens OX=9606 GN=TBC1D13 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1FC800.0e+0098.71TBC1 domain family member 5-like OS=Cucurbita moschata OX=3662 GN=LOC111444080 P... [more]
A0A6J1HSK80.0e+0097.55TBC1 domain family member 5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1HQI60.0e+0097.55TBC1 domain family member 5-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0LHC40.0e+0088.05Rab-GAP TBC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G061030... [more]
A0A5A7UD370.0e+0086.89RabGAP-TBC domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT4G29950.11.5e-20154.07Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G57210.16.4e-17358.14Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT2G19240.14.6e-17146.91Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT4G29950.25.0e-14950.52Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT2G30710.13.6e-0630.08Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000195Rab-GTPase-TBC domainSMARTSM00164tbc_4coord: 56..442
e-value: 2.1E-27
score: 107.0
IPR000195Rab-GTPase-TBC domainPFAMPF00566RabGAP-TBCcoord: 296..397
e-value: 2.8E-10
score: 40.2
coord: 143..254
e-value: 1.3E-12
score: 47.8
IPR000195Rab-GTPase-TBC domainPROSITEPS50086TBC_RABGAPcoord: 59..396
score: 25.3118
NoneNo IPR availableGENE3D1.10.472.80coord: 335..479
e-value: 2.0E-32
score: 113.8
NoneNo IPR availableGENE3D1.10.8.270putative rabgap domain of human tbc1 domain family member 14 like domainscoord: 131..312
e-value: 1.5E-14
score: 55.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 643..697
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 640..724
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 751..787
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 747..792
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 824..847
NoneNo IPR availablePANTHERPTHR22957TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEINcoord: 28..781
NoneNo IPR availablePANTHERPTHR22957:SF553YPT/RAB-GAP DOMAIN OF GYP1P SUPERFAMILY PROTEINcoord: 28..781
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 63..356
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 331..471

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg24197Carg24197gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg24197-RA:6Carg24197-RA:6exon
Carg24197-RA:5Carg24197-RA:5exon
Carg24197-RA:4Carg24197-RA:4exon
Carg24197-RA:3Carg24197-RA:3exon
Carg24197-RA:2Carg24197-RA:2exon
Carg24197-RA:1Carg24197-RA:1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg24197-RA:three_prime_utrCarg24197-RA:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg24197-RA:cdsCarg24197-RA:cds_6CDS
Carg24197-RA:cdsCarg24197-RA:cds_5CDS
Carg24197-RA:cdsCarg24197-RA:cds_4CDS
Carg24197-RA:cdsCarg24197-RA:cds_3CDS
Carg24197-RA:cdsCarg24197-RA:cds_2CDS
Carg24197-RA:cdsCarg24197-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg24197-RACarg24197-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090630 activation of GTPase activity
biological_process GO:0006886 intracellular protein transport
molecular_function GO:0005096 GTPase activator activity