Carg23870-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23870-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionE1 ubiquitin-activating enzyme
LocationCarg_Chr02: 4080164 .. 4087265 (+)
Sequence length3963
RNA-Seq ExpressionCarg23870-RA
SyntenyCarg23870-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAATTAATTTTACCTTAATTTCAATTTTAATATTAATTAATTTTGGCGTACCCAAAAATTGTGTTGAGTGCAAAAAGTCAATTCTGCCCTTCTTCCGCTTCCTTCCCTTCCATTTATCAGCCCTTGTTTTCACCTTCCATTTCCATTCATCTTCAAAGAAACCTCTCCATTCATCCTCAATTCTGAGCTTCCACTCACCCTCTCCAGATTCCTTCCTTCCTTTCTTCCTTTCTCTTTGTTACGCTCCTAATCGGTACGTTTCTTCTTCCTTTTTCCTCTTAATTAATCTCTCTTCTTTGTTTCGATTCCAAATGTCTCCCCACTCCATTCGTCTTCGTTTACGCTCCTAATCTGGCTAGGGTTTATGGTTGTTAATGCTTGGTTTCTTGATGAATTGTTTATTGGCTTTGTTTTATTCGTTGTTGTTTTGTTGATGATGTTGAGGTCTTCTGGGGTTTTCAGCAGCCTACTGCACTTTATGCTTCCCAGGAAGAGAGCTGGTGAAGAAGGTGCGGTTTTAGAACAACAAATTGATAACAGCAGTAGCAACATCAGCAACAGCGTTCAGAACGCCGGGGTGTCTCTGATCAAGAAGCACCGGATCGACAACTGTAACGTCGACTCCAACGTGAACTCTAATACCAACGTAGCTGCTGCTGTTCCCACCGGTAACAACATCGTGAACGACGGTGCATCCTTGATCATGGCATCGGGTAATTTGAACCCGCAGGATATTGATGAGGATCTGCACAGCCGACAGCTTGCCGTGTATGGTCGCGAGACCATGCGGAAGCTTTTTGCGTCCAATGTCCTCATTTCTGGAATGCAGGGTCTTGGCGCTGAGATTGGTAATTACTGACCAAAACCACCATTTTTGCTACTTTCTGCGTATTTTACTGTTGAGCACAATGGATGTTTCCTCTCTGCTGCCGGTGCCTTTTTGTTTCGAGTCATGCCAAAGCTTCTTCATTAGTCTGTGCTTTTCCTGTTAACTTGCGAGCATCTGACGATTGACCTTATTCATTTCATGGCTTCCTGATTCTGAATACATGTTCTTATATTACCCCTTTTACCTTGAAGCGGATTCTTTGTTTCTTTTATTATAGGGTTATAGCGTGGAATATCTTTACAGTTGATTTGATTTGATGATTACTTATCTTTAACTAGCTTTTTACTTGGCGGATGTCTTGTCTTCTAACTTTCTCTTTCTGTAAACATACATGCATATTCCTAGTATGAACACATTTTATTCATGTATACAAACATATGCAGTGCTTTTTATCTTGCTCCTATGGTGCATTGAAGATGCATCCGACGATGCTTTGGGCGTGGTAGATATTAATAATTTTAGATTAGAGCTGATGGTGTGTTTTAGTTTTGTTTCCCAAACTTGTCATCTTTTTTGGATTTCGTATGTGAGGGAATTTTCCCTATGCATTATTACCTGCTTTTGGTTCTCTGTGTTTGGTTTAAGTTTTCAGTTTTACTATATCTTATTTTATTGCCCCTCTTCTGCAGCAAAGAATGTTATTCTTGCTGGGGTGAAGTCTGTGACCTTGCATGACGAAGGTGTAGTGGAGTTATGGGATTTGTCTAGTAATTTTGTGTTCTCAGAGAGTGATATTGGTAAAAACAGAGCGCTTGCCTCTGCTCAGAAGTTGCAAGATCTCAACAATTCTGTCATTGTCCATACCTTGCCAACTAAATTAGTGGAAGAACAGCTTTCCAAATTCGAGGTATACCAACTTTATGCATATTTTCTACTCAGTTCTTTAAGTAGTGCACAGACTGATATGATGATATGATGATATGGAACTACATCTGATAATTCGAAAACTTACCTTACAAACTGAAAAAGGATGTGTTTCCAAAAATTGTATCCATTGGAATTGGAAGCCTAACTCGTACCATTTGTTTTCAGTCTTGTAGCAGAGACTAAAGAGCTCTAAATGAAGTATTTATATTTCTCTATTATTTTGTCTGGAGTTTTGAAAAGAACTGTAGAAATTTTGGCAATCTAATTTGAGCATTCCACAAGTATTTGTGACTCTGTTGCTTATTTTTGCTGACTTCCTATTTGAAGATCGACTTGTCTACTCATTTTATTTGTTTATAATTGTGTGCTTGTGTCTTAGATTCTTATCTCAGGTATTATTTGTATGTTTTAACCATGGTAGAGCATTTTATATATAAGCCTATTGACCACAAGATTCATGGCAGATCATCTGCCATTTGAAATGGTGATTGATACTTTTCCCAACTCCTGAACTGGCTGTTGAGTTCGAAATTCAATTTTGATTTTAGAGTTTCCACCAAACTTTTCAGAGCAGAAAAACAATGCTTCTGAAGAGCCTTGTGTTTTCTTGCTATGCTACCCCAATTTGGTCGGAATGTGCATTTAGGATGTATTTTGTCACAAGTGTTGAAGCTTGGATTAGCAAACTCTAATGGACTACTTTTAACTCTTTTAGGGAGTTGTGTTTCCCATAGTGCTGTTAGAAAGTCTATTGGCAAGTCTTCTGTAAAGAGGCCGTATTTGATAATTTTGCACATAATATGATCAGATGCATCTTGAGGCCATTGCCTGAACCTTAACTAGTAGTTGAAAAACACCTTGGTAGGCTGCCTTAACGTTTAGTCTCTCCACCTCTAAATATTGAAGTTCTTGCAAAAGTAAGGAAAAAAAAGTCCTCATTATTACGGTTCCACTATTTTAGAAATGCGACAACCTTCTTATTAGTAATAGATGACTTGGGGGCGCTTTTGAGCTAGAGGAATATCCTAAATGGTAGTTGGAAGATGGTCTTGACTTTTTATGAAGGATCTTTTGGCAAGATTTTGTTGAGGCTCATGGTTAAGCTACGTATGTAGTCTTTATTTGGTAAGTGAGCTAGAAAAGTTTCTTTAGTAATAGTATTGATTATTCCTTTTTGAAAGAGCTGCAACATTTCCATAATATCCTGCTTGAGATGGTTTCAAGACTTCTTAAAGAGAATTCAAAAGGGAAACGGTGGGCTCCGTCGTAGCCTTGCTATTTCCCTCCTATGACCTTGGAAATTTCCTGCTTAGTGAAGGTGGTGTGCTTGAGGAGTAATTCATAGCATGGTGATGCAACCCAGCATCTAGATATTGAGATTACTTGTTTACTTTTTTTTATTCATCCATAAAGTTTCCACTCAAAAACTTAATTCCCCTATTTGTGGAGATTATCATTGTAATGGATGTTTCACTAAGAAATCAATCTAGTAATTTCTTGGAAAAGAACAAGCTAACCCGACAAACGACAAATTCTAGCAATACTATCATTCTACCTCTCAGTGTCAATTTCCTTCTGTCAAAAAAAAAAAAAAAAAAAAAAAAGAGAGAAAAAGAAAATAGCTGATTTCTGTTGCAGGGGTGTACATCTTTAACATCAGTTATGTGTGCTTATTTTTCTCATGTATACGAGTCCCATGCACATTATGTAACCTTCCAAGTTCTGTGGATGCAGGTTGTTGTCTTTACTGATACTGGCCTTGACAAGGCTATGGAGTTCAATGACTTCTGTCATAACCACCAGCCTCCTATTTCATTTATCAAGTCTGAAGTCAGAGGGCTATTTGGATCAGTGTTTTGTGACTTTGGCCCGGAGTTCACTGTTTATGATGTGTATGGAGAGGACCCACACACTGGCATAATTGCGTCCATTAGCAATGACAATCCTGCACTTGTTTCCTGTGTTGATGATGAGAGGCTTGAGTTTCAGGATGGAGATCTTGTGGTGTTTTCTGAAGTTCATGGTATGACTGAACTGAATGATGGGAAGCCAAGGAGGATTAAAAATTGCAGGGCCTATTCGTTTACTCTTGATGAAGATACTACGAACTTTGGTATCTATGAAAAAGGTGGCATTGTCACACAGGTGAAGCAGCCCAAGGTGTTGAACTTCAAGCCATTAAGAGAAGCAATCAACGATCCTGGTGATTTCCTTCTGAGTGATTTCTCCAAATTTGACCGTCCTCCTCTCATACACCTGGCGTTCTTGGCATTAGATAAATTTGTGACTGAGTTGGGTCGCTTTCCAGTTGCTGGTTCTGAGGACGATGCTCAGAAGTTAATCTCTGTTGCCAGTAACATGAACAAGAGTCTAGGAGATGGGAGAGTTGAAGATATTAATCCTAAGCTTTTGAGACATTTTGCATTTGGTGCGAAGGCAGTACTGAATCCCATGGCAGCCATGTTTGGTGGTATTGTAGCTCAAGAGGTTCTCAAAGCATGCTCTGGAAAGTTTCATCCACTCGTCCAGGTTAGTAGACTAGTCATGTTTGAGGCTGAATTAAATATGCCTAATGACCTTCGTGTTTAAAAGCTTGGCGCCTTTTCATTCAAGAAGTTTCTTAAATGTCTTGACAGCCCCCATGAAGTTACTCATACAAGTTTGCTTCTCTTTGCATACATGAATTAGATCATAATTTAATGGGACCAGTGAAGTTATTGCAATGTATCTTGTTTTAACTTCCCCTCATGCTGGGATAGTGGGATTGTCATTTGATTACTATTTCTTGCATCCTTATGTTATTTTCTTTAAAAAATGCAGTTCTTCTATTTCGACTCAGTGGAGTCACTTCCCACAGAGGCATTGGACGCCAGTGAATTTAGACCCTTGAATAGCCGTTATGACGCACAGATTTCAGTGTTCGGGTCTAAGCTACAGAAGAAACTGGAAAATGCCAAAGTCTTTATGGTCGGATCTGGTGCTCTAGGTTGTGAGTTTCTGAAGAATCTTGCACTTATGGGAGTTTCATGTAGCAACGAAGGGAAGCTAACAATTACTGATGATGATGTTATTGAAAAAAGCAACCTAAGTAGGCAGTTCCTTTTCCGTGACTGGAACATTGGGCAGAACAAATCCACGGTTGCTGCTTCTGCTGCTGTTGCAATCAATCGGCACCTTAATATTGAAGCTTTGCAGAATCGAGTTAGTCCTGAAACTGAAAATGTCTTCAACGATAGCTTTTGGGAGAATTTGAGTGTCATAGTTAATGCACTAGACAACGTAAATGCAAGGCTATATGTTGATCAAAGGTGCTTATACTTCCAGAAACCACTTTTAGAATCTGGTACTCTTGGTGCTAAATGCAATACTCAAATGGTTATTCCTCACCTGACTGAAAACTATGGGGCATCAAGAGACCCACCTGAAAAACAAGCGCCCATGTGCACTGTGCATTCATTTCCGCACAATATCGATCACTGTTTGACATGGGCTCGATCCGAGTTTGAGGGTTTACTTGAGAAGACTCCTTCTGATGTGAATGCTTATCTATCTAATCCTAGTGAGTATGCTTCTTCGATGATGAATGCTGGTGATGCTCAGTCTAGGGATACTTTGGAGCGTGTTCTTGAGTGTCTTGATAGAGAAAGATGCGAGACATTTGAAGACTGCATTACATGGGCTCGATTGAAGTACTTTCCTTGATAATCTCAACTTTCATCCATATCCGTTCAACTTTTGTATGCTTCAGATCAATATATTAATTCCTGTTGTGATGATTTCATTTTGACAGGTTTGAAGATTATTTTGCAAACCGTGTAAAGCAGTTGATATACACGTTTCCTGAGGATGCTGCAACCAGTAATGGTGCCCCGTTTTGGTCGGCTCCAAAGAGATTTCCCCATCCACTTCCGTTTTCACCTGCTGATCAAAGCCACCTTCAGTTTGTTTTGGCGGCTGCTATACTAAGAGCAGAATCATATGCCATTTCAATTCCTGACTGGGTTAAGAACCCCAGGAAATTGGGCGATGCGGTCGACAGGGTTATAGTACCAGACTTTATGCCCAAAAAAGATGCCAAGATTGTGACTGATGAGAAGGCTACCAGTCTCTCTGCAGCATCTGTTGATGATGCTGCTGTTATTCATGATCTGGTAAACAAATTGGAAGATACTCGCAGGAACCTACCAGAGGGATTCAGGATGAAACCGATCCAGTTTGAGAAGGTATTTCTTCTTTTTTAATGAAAAGAAAATACAAGTAGAGTGATATTTATGACTGACTGTAAAGGTTTATTAGAATCAATAATGTAAATTCAACTAGCGAGTTTGGACGTGCAGGATGATGATACAAATTACCATATGGACCTCATAGCTGGACTGGCTAACATGAGAGCAAGAAATTACAGCATTCCTGAAGTAGATAAGTTGAAAGCCAAGTTCATTGCCGGAAGGATCATCCCCGCCATTGCAACCTCTACTGCAATGGCCACGGGTCTTGTCTGCCTTGAACTCTACAAAGTTCTAGATGGCGGCCACAAGGTGGAGGACTACCGTAACACATTTGCCAACCTTGCATTGCCTCTGTTCTCCATGGCTGAGCCAGTCCCACCCAAGGTCATTAAGCACCGTGACATGAGCTGGACTGTTTGGGACAGATGGATCATCAAAGACAACCCTACTCTCAGAGAACTCATTAAATGGTTGAAGAACAAGGGATTGAACGCCTATAGCATCTCATGTGGTAGCTGTCTCCTGTACAATAGCATGTTTCCTCGACACAAAGATCGAATGGACAAGAAGGTAGTTGATTTAGCTCGAGACATTGCCAAGGTGGAACTGCCCCCGTATCGTCGACATTTGGATGTCGTGGTGGCATGCGAGGATGATGAGGATAATGATATTGACATCCCACTGGTGTCTGTTTACTTTTAGTTAGGTACTACTCTTCATCACTGGATCATTCTTCTGTCCCTGGCAAGACCTTCTTTTTAGTCTTTAAAAGTCTAGAGCTGCGAGAGTATTCAAATATGAAACGGCCAACACACAGTTCACATATTCATACATAAATGTGGAAGAAACAAGTTGATTTTGTGCAATATGAATGATGTACCCTTATTGTATGGTTGGTTGAAGCTATATCCACCAACACTGGCTTCGAGATGTTCGACTTTTTTTCATGAAGATGAGTTGAGTTTTTCGCTTTACACATTTCAACATTGTTATTTCTCGTCTTGTCCTCTCCTCTGCACTTTTTCCTCCCTTCTATCCATCGAGTATTGGATGTTTAAATTATTGAAAATTGGGTTTAGTATGTTCTTGGAAATAAAAAATGTGTTTGGGTGCTTT

mRNA sequence

GTAATTAATTTTACCTTAATTTCAATTTTAATATTAATTAATTTTGGCGTACCCAAAAATTGTGTTGAGTGCAAAAAGTCAATTCTGCCCTTCTTCCGCTTCCTTCCCTTCCATTTATCAGCCCTTGTTTTCACCTTCCATTTCCATTCATCTTCAAAGAAACCTCTCCATTCATCCTCAATTCTGAGCTTCCACTCACCCTCTCCAGATTCCTTCCTTCCTTTCTTCCTTTCTCTTTGTTACGCTCCTAATCGCAGCCTACTGCACTTTATGCTTCCCAGGAAGAGAGCTGGTGAAGAAGGTGCGGTTTTAGAACAACAAATTGATAACAGCAGTAGCAACATCAGCAACAGCGTTCAGAACGCCGGGGTGTCTCTGATCAAGAAGCACCGGATCGACAACTGTAACGTCGACTCCAACGTGAACTCTAATACCAACGTAGCTGCTGCTGTTCCCACCGGTAACAACATCGTGAACGACGGTGCATCCTTGATCATGGCATCGGGTAATTTGAACCCGCAGGATATTGATGAGGATCTGCACAGCCGACAGCTTGCCGTGTATGGTCGCGAGACCATGCGGAAGCTTTTTGCGTCCAATGTCCTCATTTCTGGAATGCAGGGTCTTGGCGCTGAGATTGCAAAGAATGTTATTCTTGCTGGGGTGAAGTCTGTGACCTTGCATGACGAAGGTGTAGTGGAGTTATGGGATTTGTCTAGTAATTTTGTGTTCTCAGAGAGTGATATTGGTAAAAACAGAGCGCTTGCCTCTGCTCAGAAGTTGCAAGATCTCAACAATTCTGTCATTGTCCATACCTTGCCAACTAAATTAGTGGAAGAACAGCTTTCCAAATTCGAGGTTGTTGTCTTTACTGATACTGGCCTTGACAAGGCTATGGAGTTCAATGACTTCTGTCATAACCACCAGCCTCCTATTTCATTTATCAAGTCTGAAGTCAGAGGGCTATTTGGATCAGTGTTTTGTGACTTTGGCCCGGAGTTCACTGTTTATGATGTGTATGGAGAGGACCCACACACTGGCATAATTGCGTCCATTAGCAATGACAATCCTGCACTTGTTTCCTGTGTTGATGATGAGAGGCTTGAGTTTCAGGATGGAGATCTTGTGGTGTTTTCTGAAGTTCATGGTATGACTGAACTGAATGATGGGAAGCCAAGGAGGATTAAAAATTGCAGGGCCTATTCGTTTACTCTTGATGAAGATACTACGAACTTTGGTATCTATGAAAAAGGTGGCATTGTCACACAGGTGAAGCAGCCCAAGGTGTTGAACTTCAAGCCATTAAGAGAAGCAATCAACGATCCTGGTGATTTCCTTCTGAGTGATTTCTCCAAATTTGACCGTCCTCCTCTCATACACCTGGCGTTCTTGGCATTAGATAAATTTGTGACTGAGTTGGGTCGCTTTCCAGTTGCTGGTTCTGAGGACGATGCTCAGAAGTTAATCTCTGTTGCCAGTAACATGAACAAGAGTCTAGGAGATGGGAGAGTTGAAGATATTAATCCTAAGCTTTTGAGACATTTTGCATTTGGTGCGAAGGCAGTACTGAATCCCATGGCAGCCATGTTTGGTGGTATTGTAGCTCAAGAGGTTCTCAAAGCATGCTCTGGAAAGTTTCATCCACTCGTCCAGTTCTTCTATTTCGACTCAGTGGAGTCACTTCCCACAGAGGCATTGGACGCCAGTGAATTTAGACCCTTGAATAGCCGTTATGACGCACAGATTTCAGTGTTCGGGTCTAAGCTACAGAAGAAACTGGAAAATGCCAAAGTCTTTATGGTCGGATCTGGTGCTCTAGGTTGTGAGTTTCTGAAGAATCTTGCACTTATGGGAGTTTCATGTAGCAACGAAGGGAAGCTAACAATTACTGATGATGATGTTATTGAAAAAAGCAACCTAAGTAGGCAGTTCCTTTTCCGTGACTGGAACATTGGGCAGAACAAATCCACGGTTGCTGCTTCTGCTGCTGTTGCAATCAATCGGCACCTTAATATTGAAGCTTTGCAGAATCGAGTTAGTCCTGAAACTGAAAATGTCTTCAACGATAGCTTTTGGGAGAATTTGAGTGTCATAGTTAATGCACTAGACAACGTAAATGCAAGGCTATATGTTGATCAAAGGTGCTTATACTTCCAGAAACCACTTTTAGAATCTGGTACTCTTGGTGCTAAATGCAATACTCAAATGGTTATTCCTCACCTGACTGAAAACTATGGGGCATCAAGAGACCCACCTGAAAAACAAGCGCCCATGTGCACTGTGCATTCATTTCCGCACAATATCGATCACTGTTTGACATGGGCTCGATCCGAGTTTGAGGGTTTACTTGAGAAGACTCCTTCTGATGTGAATGCTTATCTATCTAATCCTAGTGAGTATGCTTCTTCGATGATGAATGCTGGTGATGCTCAGTCTAGGGATACTTTGGAGCGTGTTCTTGAGTGTCTTGATAGAGAAAGATGCGAGACATTTGAAGACTGCATTACATGGGCTCGATTGAAGTTTGAAGATTATTTTGCAAACCGTGTAAAGCAGTTGATATACACGTTTCCTGAGGATGCTGCAACCAGTAATGGTGCCCCGTTTTGGTCGGCTCCAAAGAGATTTCCCCATCCACTTCCGTTTTCACCTGCTGATCAAAGCCACCTTCAGTTTGTTTTGGCGGCTGCTATACTAAGAGCAGAATCATATGCCATTTCAATTCCTGACTGGGTTAAGAACCCCAGGAAATTGGGCGATGCGGTCGACAGGGTTATAGTACCAGACTTTATGCCCAAAAAAGATGCCAAGATTGTGACTGATGAGAAGGCTACCAGTCTCTCTGCAGCATCTGTTGATGATGCTGCTGTTATTCATGATCTGGTAAACAAATTGGAAGATACTCGCAGGAACCTACCAGAGGGATTCAGGATGAAACCGATCCAGTTTGAGAAGGATGATGATACAAATTACCATATGGACCTCATAGCTGGACTGGCTAACATGAGAGCAAGAAATTACAGCATTCCTGAAGTAGATAAGTTGAAAGCCAAGTTCATTGCCGGAAGGATCATCCCCGCCATTGCAACCTCTACTGCAATGGCCACGGGTCTTGTCTGCCTTGAACTCTACAAAGTTCTAGATGGCGGCCACAAGGTGGAGGACTACCGTAACACATTTGCCAACCTTGCATTGCCTCTGTTCTCCATGGCTGAGCCAGTCCCACCCAAGGTCATTAAGCACCGTGACATGAGCTGGACTGTTTGGGACAGATGGATCATCAAAGACAACCCTACTCTCAGAGAACTCATTAAATGGTTGAAGAACAAGGGATTGAACGCCTATAGCATCTCATGTGGTAGCTGTCTCCTGTACAATAGCATGTTTCCTCGACACAAAGATCGAATGGACAAGAAGGTAGTTGATTTAGCTCGAGACATTGCCAAGGTGGAACTGCCCCCGTATCGTCGACATTTGGATGTCGTGGTGGCATGCGAGGATGATGAGGATAATGATATTGACATCCCACTGGTGTCTGTTTACTTTTAGTTAGGTACTACTCTTCATCACTGGATCATTCTTCTGTCCCTGGCAAGACCTTCTTTTTAGTCTTTAAAAGTCTAGAGCTGCGAGAGTATTCAAATATGAAACGGCCAACACACAGTTCACATATTCATACATAAATGTGGAAGAAACAAGTTGATTTTGTGCAATATGAATGATGTACCCTTATTGTATGGTTGGTTGAAGCTATATCCACCAACACTGGCTTCGAGATGTTCGACTTTTTTTCATGAAGATGAGTTGAGTTTTTCGCTTTACACATTTCAACATTGTTATTTCTCGTCTTGTCCTCTCCTCTGCACTTTTTCCTCCCTTCTATCCATCGAGTATTGGATGTTTAAATTATTGAAAATTGGGTTTAGTATGTTCTTGGAAATAAAAAATGTGTTTGGGTGCTTT

Coding sequence (CDS)

GTAATTAATTTTACCTTAATTTCAATTTTAATATTAATTAATTTTGGCGTACCCAAAAATTGTGTTGAGTGCAAAAAGTCAATTCTGCCCTTCTTCCGCTTCCTTCCCTTCCATTTATCAGCCCTTGTTTTCACCTTCCATTTCCATTCATCTTCAAAGAAACCTCTCCATTCATCCTCAATTCTGAGCTTCCACTCACCCTCTCCAGATTCCTTCCTTCCTTTCTTCCTTTCTCTTTGTTACGCTCCTAATCGCAGCCTACTGCACTTTATGCTTCCCAGGAAGAGAGCTGGTGAAGAAGGTGCGGTTTTAGAACAACAAATTGATAACAGCAGTAGCAACATCAGCAACAGCGTTCAGAACGCCGGGGTGTCTCTGATCAAGAAGCACCGGATCGACAACTGTAACGTCGACTCCAACGTGAACTCTAATACCAACGTAGCTGCTGCTGTTCCCACCGGTAACAACATCGTGAACGACGGTGCATCCTTGATCATGGCATCGGGTAATTTGAACCCGCAGGATATTGATGAGGATCTGCACAGCCGACAGCTTGCCGTGTATGGTCGCGAGACCATGCGGAAGCTTTTTGCGTCCAATGTCCTCATTTCTGGAATGCAGGGTCTTGGCGCTGAGATTGCAAAGAATGTTATTCTTGCTGGGGTGAAGTCTGTGACCTTGCATGACGAAGGTGTAGTGGAGTTATGGGATTTGTCTAGTAATTTTGTGTTCTCAGAGAGTGATATTGGTAAAAACAGAGCGCTTGCCTCTGCTCAGAAGTTGCAAGATCTCAACAATTCTGTCATTGTCCATACCTTGCCAACTAAATTAGTGGAAGAACAGCTTTCCAAATTCGAGGTTGTTGTCTTTACTGATACTGGCCTTGACAAGGCTATGGAGTTCAATGACTTCTGTCATAACCACCAGCCTCCTATTTCATTTATCAAGTCTGAAGTCAGAGGGCTATTTGGATCAGTGTTTTGTGACTTTGGCCCGGAGTTCACTGTTTATGATGTGTATGGAGAGGACCCACACACTGGCATAATTGCGTCCATTAGCAATGACAATCCTGCACTTGTTTCCTGTGTTGATGATGAGAGGCTTGAGTTTCAGGATGGAGATCTTGTGGTGTTTTCTGAAGTTCATGGTATGACTGAACTGAATGATGGGAAGCCAAGGAGGATTAAAAATTGCAGGGCCTATTCGTTTACTCTTGATGAAGATACTACGAACTTTGGTATCTATGAAAAAGGTGGCATTGTCACACAGGTGAAGCAGCCCAAGGTGTTGAACTTCAAGCCATTAAGAGAAGCAATCAACGATCCTGGTGATTTCCTTCTGAGTGATTTCTCCAAATTTGACCGTCCTCCTCTCATACACCTGGCGTTCTTGGCATTAGATAAATTTGTGACTGAGTTGGGTCGCTTTCCAGTTGCTGGTTCTGAGGACGATGCTCAGAAGTTAATCTCTGTTGCCAGTAACATGAACAAGAGTCTAGGAGATGGGAGAGTTGAAGATATTAATCCTAAGCTTTTGAGACATTTTGCATTTGGTGCGAAGGCAGTACTGAATCCCATGGCAGCCATGTTTGGTGGTATTGTAGCTCAAGAGGTTCTCAAAGCATGCTCTGGAAAGTTTCATCCACTCGTCCAGTTCTTCTATTTCGACTCAGTGGAGTCACTTCCCACAGAGGCATTGGACGCCAGTGAATTTAGACCCTTGAATAGCCGTTATGACGCACAGATTTCAGTGTTCGGGTCTAAGCTACAGAAGAAACTGGAAAATGCCAAAGTCTTTATGGTCGGATCTGGTGCTCTAGGTTGTGAGTTTCTGAAGAATCTTGCACTTATGGGAGTTTCATGTAGCAACGAAGGGAAGCTAACAATTACTGATGATGATGTTATTGAAAAAAGCAACCTAAGTAGGCAGTTCCTTTTCCGTGACTGGAACATTGGGCAGAACAAATCCACGGTTGCTGCTTCTGCTGCTGTTGCAATCAATCGGCACCTTAATATTGAAGCTTTGCAGAATCGAGTTAGTCCTGAAACTGAAAATGTCTTCAACGATAGCTTTTGGGAGAATTTGAGTGTCATAGTTAATGCACTAGACAACGTAAATGCAAGGCTATATGTTGATCAAAGGTGCTTATACTTCCAGAAACCACTTTTAGAATCTGGTACTCTTGGTGCTAAATGCAATACTCAAATGGTTATTCCTCACCTGACTGAAAACTATGGGGCATCAAGAGACCCACCTGAAAAACAAGCGCCCATGTGCACTGTGCATTCATTTCCGCACAATATCGATCACTGTTTGACATGGGCTCGATCCGAGTTTGAGGGTTTACTTGAGAAGACTCCTTCTGATGTGAATGCTTATCTATCTAATCCTAGTGAGTATGCTTCTTCGATGATGAATGCTGGTGATGCTCAGTCTAGGGATACTTTGGAGCGTGTTCTTGAGTGTCTTGATAGAGAAAGATGCGAGACATTTGAAGACTGCATTACATGGGCTCGATTGAAGTTTGAAGATTATTTTGCAAACCGTGTAAAGCAGTTGATATACACGTTTCCTGAGGATGCTGCAACCAGTAATGGTGCCCCGTTTTGGTCGGCTCCAAAGAGATTTCCCCATCCACTTCCGTTTTCACCTGCTGATCAAAGCCACCTTCAGTTTGTTTTGGCGGCTGCTATACTAAGAGCAGAATCATATGCCATTTCAATTCCTGACTGGGTTAAGAACCCCAGGAAATTGGGCGATGCGGTCGACAGGGTTATAGTACCAGACTTTATGCCCAAAAAAGATGCCAAGATTGTGACTGATGAGAAGGCTACCAGTCTCTCTGCAGCATCTGTTGATGATGCTGCTGTTATTCATGATCTGGTAAACAAATTGGAAGATACTCGCAGGAACCTACCAGAGGGATTCAGGATGAAACCGATCCAGTTTGAGAAGGATGATGATACAAATTACCATATGGACCTCATAGCTGGACTGGCTAACATGAGAGCAAGAAATTACAGCATTCCTGAAGTAGATAAGTTGAAAGCCAAGTTCATTGCCGGAAGGATCATCCCCGCCATTGCAACCTCTACTGCAATGGCCACGGGTCTTGTCTGCCTTGAACTCTACAAAGTTCTAGATGGCGGCCACAAGGTGGAGGACTACCGTAACACATTTGCCAACCTTGCATTGCCTCTGTTCTCCATGGCTGAGCCAGTCCCACCCAAGGTCATTAAGCACCGTGACATGAGCTGGACTGTTTGGGACAGATGGATCATCAAAGACAACCCTACTCTCAGAGAACTCATTAAATGGTTGAAGAACAAGGGATTGAACGCCTATAGCATCTCATGTGGTAGCTGTCTCCTGTACAATAGCATGTTTCCTCGACACAAAGATCGAATGGACAAGAAGGTAGTTGATTTAGCTCGAGACATTGCCAAGGTGGAACTGCCCCCGTATCGTCGACATTTGGATGTCGTGGTGGCATGCGAGGATGATGAGGATAATGATATTGACATCCCACTGGTGTCTGTTTACTTTTAG

Protein sequence

VINFTLISILILINFGVPKNCVECKKSILPFFRFLPFHLSALVFTFHFHSSSKKPLHSSSILSFHSPSPDSFLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF
Homology
BLAST of Carg23870-RA vs. NCBI nr
Match: KAG7035346.1 (Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2375.9 bits (6156), Expect = 0.0e+00
Identity = 1182/1182 (100.00%), Postives = 1182/1182 (100.00%), Query Frame = 0

Query: 1    VINFTLISILILINFGVPKNCVECKKSILPFFRFLPFHLSALVFTFHFHSSSKKPLHSSS 60
            VINFTLISILILINFGVPKNCVECKKSILPFFRFLPFHLSALVFTFHFHSSSKKPLHSSS
Sbjct: 1    VINFTLISILILINFGVPKNCVECKKSILPFFRFLPFHLSALVFTFHFHSSSKKPLHSSS 60

Query: 61   ILSFHSPSPDSFLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQIDNSSSNISNSVQ 120
            ILSFHSPSPDSFLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQIDNSSSNISNSVQ
Sbjct: 61   ILSFHSPSPDSFLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQIDNSSSNISNSVQ 120

Query: 121  NAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASGNLNPQDIDEDL 180
            NAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASGNLNPQDIDEDL
Sbjct: 121  NAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASGNLNPQDIDEDL 180

Query: 181  HSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSS 240
            HSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSS
Sbjct: 181  HSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSS 240

Query: 241  NFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAME 300
            NFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAME
Sbjct: 241  NFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAME 300

Query: 301  FNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALV 360
            FNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALV
Sbjct: 301  FNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALV 360

Query: 361  SCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGI 420
            SCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGI
Sbjct: 361  SCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGI 420

Query: 421  VTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAG 480
            VTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAG
Sbjct: 421  VTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAG 480

Query: 481  SEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLK 540
            SEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLK
Sbjct: 481  SEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLK 540

Query: 541  ACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFM 600
            ACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFM
Sbjct: 541  ACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFM 600

Query: 601  VGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAA 660
            VGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAA
Sbjct: 601  VGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAA 660

Query: 661  SAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQK 720
            SAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQK
Sbjct: 661  SAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQK 720

Query: 721  PLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFE 780
            PLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFE
Sbjct: 721  PLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFE 780

Query: 781  GLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFEDCITWARL 840
            GLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFEDCITWARL
Sbjct: 781  GLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFEDCITWARL 840

Query: 841  KFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILR 900
            KFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILR
Sbjct: 841  KFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILR 900

Query: 901  AESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHD 960
            AESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHD
Sbjct: 901  AESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHD 960

Query: 961  LVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFI 1020
            LVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFI
Sbjct: 961  LVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFI 1020

Query: 1021 AGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIK 1080
            AGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIK
Sbjct: 1021 AGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIK 1080

Query: 1081 HRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKV 1140
            HRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKV
Sbjct: 1081 HRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKV 1140

Query: 1141 VDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
            VDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF
Sbjct: 1141 VDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1182

BLAST of Carg23870-RA vs. NCBI nr
Match: KAG6605397.1 (Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2265.7 bits (5870), Expect = 0.0e+00
Identity = 1127/1133 (99.47%), Postives = 1130/1133 (99.74%), Query Frame = 0

Query: 50   SSSKKPLHSSSILSFHSPSPDSFLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQID 109
            +S  KPLHSSSILSFHSPSPDSFLPFFLSLCYAPNR LLHFMLPRKRAGEEGAVLEQQID
Sbjct: 1134 TSHNKPLHSSSILSFHSPSPDSFLPFFLSLCYAPNR-LLHFMLPRKRAGEEGAVLEQQID 1193

Query: 110  NSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASG 169
            NSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASG
Sbjct: 1194 NSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASG 1253

Query: 170  NLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHD 229
            NLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHD
Sbjct: 1254 NLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHD 1313

Query: 230  EGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVV 289
            EGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLV+EQLSKFEVVV
Sbjct: 1314 EGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVKEQLSKFEVVV 1373

Query: 290  FTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 349
            FTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII
Sbjct: 1374 FTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 1433

Query: 350  ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDT 409
            ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDT
Sbjct: 1434 ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDT 1493

Query: 410  TNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKF 469
            TNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKF
Sbjct: 1494 TNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKF 1553

Query: 470  VTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 529
            VTELGRFPVAGSEDDAQKLISVASNMN+SLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM
Sbjct: 1554 VTELGRFPVAGSEDDAQKLISVASNMNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 1613

Query: 530  FGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKL 589
            FGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKL
Sbjct: 1614 FGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKL 1673

Query: 590  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 649
            QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW
Sbjct: 1674 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 1733

Query: 650  NIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARL 709
            NIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARL
Sbjct: 1734 NIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARL 1793

Query: 710  YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 769
            YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID
Sbjct: 1794 YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 1853

Query: 770  HCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCE 829
            HCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCE
Sbjct: 1854 HCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCE 1913

Query: 830  TFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSH 889
            TFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSH
Sbjct: 1914 TFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSH 1973

Query: 890  LQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSA 949
            LQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSA
Sbjct: 1974 LQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSA 2033

Query: 950  ASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSI 1009
            ASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSI
Sbjct: 2034 ASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSI 2093

Query: 1010 PEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFS 1069
            PEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFS
Sbjct: 2094 PEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFS 2153

Query: 1070 MAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMF 1129
            MAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMF
Sbjct: 2154 MAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMF 2213

Query: 1130 PRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
            PRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF
Sbjct: 2214 PRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 2265

BLAST of Carg23870-RA vs. NCBI nr
Match: XP_022947321.1 (ubiquitin-activating enzyme E1 1-like [Cucurbita moschata] >XP_022947322.1 ubiquitin-activating enzyme E1 1-like [Cucurbita moschata] >XP_022947323.1 ubiquitin-activating enzyme E1 1-like [Cucurbita moschata])

HSP 1 Score: 2200.2 bits (5700), Expect = 0.0e+00
Identity = 1092/1092 (100.00%), Postives = 1092/1092 (100.00%), Query Frame = 0

Query: 91   MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 150
            MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA
Sbjct: 1    MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 60

Query: 151  VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 210
            VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG
Sbjct: 61   VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 120

Query: 211  AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 270
            AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV
Sbjct: 121  AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 180

Query: 271  HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 330
            HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF
Sbjct: 181  HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 240

Query: 331  GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 390
            GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG
Sbjct: 241  GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 300

Query: 391  KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 450
            KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF
Sbjct: 301  KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 360

Query: 451  SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK 510
            SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK
Sbjct: 361  SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK 420

Query: 511  LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 570
            LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE
Sbjct: 421  LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 480

Query: 571  FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 630
            FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT
Sbjct: 481  FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 540

Query: 631  DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 690
            DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS
Sbjct: 541  DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 600

Query: 691  FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 750
            FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD
Sbjct: 601  FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 660

Query: 751  PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 810
            PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA
Sbjct: 661  PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 720

Query: 811  QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 870
            QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW
Sbjct: 721  QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 780

Query: 871  SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF 930
            SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF
Sbjct: 781  SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF 840

Query: 931  MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 990
            MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN
Sbjct: 841  MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 900

Query: 991  YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 1050
            YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG
Sbjct: 901  YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 960

Query: 1051 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1110
            HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK
Sbjct: 961  HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1020

Query: 1111 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1170
            GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED
Sbjct: 1021 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1080

Query: 1171 NDIDIPLVSVYF 1183
            NDIDIPLVSVYF
Sbjct: 1081 NDIDIPLVSVYF 1092

BLAST of Carg23870-RA vs. NCBI nr
Match: XP_023532141.1 (ubiquitin-activating enzyme E1 1-like [Cucurbita pepo subsp. pepo] >XP_023532142.1 ubiquitin-activating enzyme E1 1-like [Cucurbita pepo subsp. pepo] >XP_023532143.1 ubiquitin-activating enzyme E1 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2172.9 bits (5629), Expect = 0.0e+00
Identity = 1081/1092 (98.99%), Postives = 1085/1092 (99.36%), Query Frame = 0

Query: 91   MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 150
            MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA
Sbjct: 1    MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 60

Query: 151  VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 210
            VPT NNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG
Sbjct: 61   VPTANNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 120

Query: 211  AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 270
            AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV
Sbjct: 121  AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 180

Query: 271  HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 330
            HTL TKLV+EQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF
Sbjct: 181  HTLATKLVKEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 240

Query: 331  GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 390
            GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG
Sbjct: 241  GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 300

Query: 391  KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 450
            KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF
Sbjct: 301  KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 360

Query: 451  SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK 510
            SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMN+SLGDGRVEDIN K
Sbjct: 361  SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNESLGDGRVEDINLK 420

Query: 511  LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 570
            LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE
Sbjct: 421  LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 480

Query: 571  FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 630
            FRPLNSRYDAQISVFGSKLQKKLENA+VFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT
Sbjct: 481  FRPLNSRYDAQISVFGSKLQKKLENAQVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 540

Query: 631  DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 690
            DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS
Sbjct: 541  DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 600

Query: 691  FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 750
            FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD
Sbjct: 601  FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 660

Query: 751  PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 810
            PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA
Sbjct: 661  PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 720

Query: 811  QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 870
            QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW
Sbjct: 721  QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 780

Query: 871  SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF 930
            SAPKRFPHPLPFS ADQSHLQFVLAAAILRAESYAISIPDWVKN  KL DAVDRVIVPDF
Sbjct: 781  SAPKRFPHPLPFSTADQSHLQFVLAAAILRAESYAISIPDWVKNHGKLADAVDRVIVPDF 840

Query: 931  MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 990
            MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN
Sbjct: 841  MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 900

Query: 991  YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 1050
            YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG
Sbjct: 901  YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 960

Query: 1051 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1110
            HKVE+YRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK
Sbjct: 961  HKVENYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1020

Query: 1111 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1170
            GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED
Sbjct: 1021 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1080

Query: 1171 NDIDIPLVSVYF 1183
            NDIDIPLVSVYF
Sbjct: 1081 NDIDIPLVSVYF 1092

BLAST of Carg23870-RA vs. NCBI nr
Match: XP_023006875.1 (ubiquitin-activating enzyme E1 1-like [Cucurbita maxima] >XP_023006876.1 ubiquitin-activating enzyme E1 1-like [Cucurbita maxima] >XP_023006877.1 ubiquitin-activating enzyme E1 1-like [Cucurbita maxima])

HSP 1 Score: 2172.1 bits (5627), Expect = 0.0e+00
Identity = 1081/1095 (98.72%), Postives = 1087/1095 (99.27%), Query Frame = 0

Query: 91   MLPRKRAGEEGAVLEQQIDNSS---SNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNV 150
            MLPRKRAGEEGAVLEQQIDNSS   SNISNSVQNAGVSLIKKHRIDNCNVDSNVN NTNV
Sbjct: 1    MLPRKRAGEEGAVLEQQIDNSSSNISNISNSVQNAGVSLIKKHRIDNCNVDSNVNFNTNV 60

Query: 151  AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 210
            AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ
Sbjct: 61   AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 120

Query: 211  GLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS 270
            GLGAEIAKNVILAGVKSVTLHD+GVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS
Sbjct: 121  GLGAEIAKNVILAGVKSVTLHDDGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS 180

Query: 271  VIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF 330
            VIVHTL TKLV+EQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF
Sbjct: 181  VIVHTLTTKLVKEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF 240

Query: 331  CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL 390
            CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL
Sbjct: 241  CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL 300

Query: 391  NDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL 450
            NDGKPRRIKNCRAYSFTLDEDTTN+GIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL
Sbjct: 301  NDGKPRRIKNCRAYSFTLDEDTTNYGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL 360

Query: 451  SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDI 510
            SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMN+SLGDGRVEDI
Sbjct: 361  SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNESLGDGRVEDI 420

Query: 511  NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD 570
            NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD
Sbjct: 421  NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD 480

Query: 571  ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL 630
            ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL
Sbjct: 481  ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL 540

Query: 631  TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF 690
            TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF
Sbjct: 541  TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF 600

Query: 691  NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA 750
            NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA
Sbjct: 601  NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA 660

Query: 751  SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA 810
            SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA
Sbjct: 661  SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA 720

Query: 811  GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA 870
            GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA
Sbjct: 721  GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA 780

Query: 871  PFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIV 930
            PFWSAPKRFPHPLPFS ADQSHLQFVLAAAILRAESYAISIPDWVKNPRKL DAVDRVIV
Sbjct: 781  PFWSAPKRFPHPLPFSTADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLADAVDRVIV 840

Query: 931  PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDD 990
            PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLV+KLEDTRRNLPEGFRMKPIQFEKDD
Sbjct: 841  PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVDKLEDTRRNLPEGFRMKPIQFEKDD 900

Query: 991  DTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL 1050
            DTNYHMDLIAGLANMRARN+SIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL
Sbjct: 901  DTNYHMDLIAGLANMRARNFSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL 960

Query: 1051 DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL 1110
            DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL
Sbjct: 961  DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL 1020

Query: 1111 KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACED 1170
            KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPY RHLDVVVACED
Sbjct: 1021 KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYCRHLDVVVACED 1080

Query: 1171 DEDNDIDIPLVSVYF 1183
            DEDNDIDIPLVSVYF
Sbjct: 1081 DEDNDIDIPLVSVYF 1095

BLAST of Carg23870-RA vs. ExPASy Swiss-Prot
Match: P93028 (Ubiquitin-activating enzyme E1 1 OS=Arabidopsis thaliana OX=3702 GN=UBA1 PE=1 SV=1)

HSP 1 Score: 1726.5 bits (4470), Expect = 0.0e+00
Identity = 834/1080 (77.22%), Postives = 956/1080 (88.52%), Query Frame = 0

Query: 103  VLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGA 162
            +L ++   ++    N++  + ++  KK RID     S+  S++ +A+    G +  +   
Sbjct: 1    MLHKRASEANDKNDNTIIGSDLASSKKRRIDFTESSSD-KSSSILASGSSRGFHGDSVVQ 60

Query: 163  SLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGV 222
             + MA GN N Q+IDEDLHSRQLAVYGRETMR+LFASNVLISGM GLGAEIAKN+ILAGV
Sbjct: 61   QIDMAFGNSNRQEIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEIAKNLILAGV 120

Query: 223  KSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQL 282
            KSVTLHDE VVELWDLSSNFVFSE D+GKNRA AS QKLQDLNN+V+V +L   L +E L
Sbjct: 121  KSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSSLTKSLNKEDL 180

Query: 283  SKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGE 342
            S F+VVVF+D  +++A+EF+D+CH+HQPPI+F+K++VRGLFGSVFCDFGPEF V DV GE
Sbjct: 181  SGFQVVVFSDISMERAIEFDDYCHSHQPPIAFVKADVRGLFGSVFCDFGPEFAVLDVDGE 240

Query: 343  DPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYS 402
            +PHTGIIASISN+N A +SCVDDERLEF+DGDLVVFSEV GMTELNDGKPR+IK+ R YS
Sbjct: 241  EPHTGIIASISNENQAFISCVDDERLEFEDGDLVVFSEVEGMTELNDGKPRKIKSTRPYS 300

Query: 403  FTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLA 462
            FTLDEDTTN+G Y KGGIVTQVKQPK+LNFKPLREA+ DPGDFL SDFSKFDRPPL+HLA
Sbjct: 301  FTLDEDTTNYGTYVKGGIVTQVKQPKLLNFKPLREALKDPGDFLFSDFSKFDRPPLLHLA 360

Query: 463  FLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAV 522
            F ALD F  E GRFPVAGSE+DAQKLIS+A+ +N   GD +VE+++ KLLRHF+FGAKAV
Sbjct: 361  FQALDHFKAEAGRFPVAGSEEDAQKLISIATAINTGQGDLKVENVDQKLLRHFSFGAKAV 420

Query: 523  LNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQI 582
            LNPMAAMFGGIV QEV+KACSGKFHPL QFFYFDSVESLP+E +D+S+F P NSRYDAQI
Sbjct: 421  LNPMAAMFGGIVGQEVVKACSGKFHPLFQFFYFDSVESLPSEPVDSSDFAPRNSRYDAQI 480

Query: 583  SVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSR 642
            SVFG+K QKKLE+AKVF VGSGALGCEFLKNLALMGVSC ++GKLT+TDDD+IEKSNLSR
Sbjct: 481  SVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGVSCGSQGKLTVTDDDIIEKSNLSR 540

Query: 643  QFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNAL 702
            QFLFRDWNIGQ KSTVAASAA  IN   NIEALQNRV  ETENVF+D+FWENL+V+VNAL
Sbjct: 541  QFLFRDWNIGQAKSTVAASAAAVINPRFNIEALQNRVGAETENVFDDAFWENLTVVVNAL 600

Query: 703  DNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVH 762
            DNVNARLYVD RCLYFQKPLLESGTLG KCNTQ VIPHLTENYGASRDPPEKQAPMCTVH
Sbjct: 601  DNVNARLYVDSRCLYFQKPLLESGTLGTKCNTQSVIPHLTENYGASRDPPEKQAPMCTVH 660

Query: 763  SFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLEC 822
            SFPHNIDHCLTWARSEFEGLLEKTP++VNAYLS+P EY +SMM+AGDAQ+RDTLER++EC
Sbjct: 661  SFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSSPVEYTNSMMSAGDAQARDTLERIVEC 720

Query: 823  LDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPF 882
            L++E+CETF+DC+TWARL+FEDYF NRVKQLIYTFPEDAATS GAPFWSAPKRFP PL +
Sbjct: 721  LEKEKCETFQDCLTWARLRFEDYFVNRVKQLIYTFPEDAATSTGAPFWSAPKRFPRPLQY 780

Query: 883  SPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDE 942
            S +D S L F+ A AILRAE++ I IP+W KNP++  +AVDRVIVPDF P++DAKIVTDE
Sbjct: 781  SSSDPSLLNFITATAILRAETFGIPIPEWTKNPKEAAEAVDRVIVPDFEPRQDAKIVTDE 840

Query: 943  KATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANM 1002
            KAT+L+ ASVDDAAVI DL+ K++  R NL   FRMKPIQFEKDDDTNYHMD+IAGLANM
Sbjct: 841  KATTLTTASVDDAAVIDDLIAKIDQCRHNLSPDFRMKPIQFEKDDDTNYHMDVIAGLANM 900

Query: 1003 RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFAN 1062
            RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVE YRNTFAN
Sbjct: 901  RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEAYRNTFAN 960

Query: 1063 LALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSC 1122
            LALPLFSMAEP+PPKV+KHRDM+WTVWDRW++K NPTLRE+++WL++KGL+AYSISCGSC
Sbjct: 961  LALPLFSMAEPLPPKVVKHRDMAWTVWDRWVLKGNPTLREVLQWLEDKGLSAYSISCGSC 1020

Query: 1123 LLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1182
            LL+NSMF RHK+RMDKKVVDLARD+AKVELPPYR HLDVVVACED++DND+DIPLVS+YF
Sbjct: 1021 LLFNSMFTRHKERMDKKVVDLARDVAKVELPPYRNHLDVVVACEDEDDNDVDIPLVSIYF 1079

BLAST of Carg23870-RA vs. ExPASy Swiss-Prot
Match: P92974 (Ubiquitin-activating enzyme E1 2 OS=Arabidopsis thaliana OX=3702 GN=UBA2 PE=1 SV=1)

HSP 1 Score: 1694.1 bits (4386), Expect = 0.0e+00
Identity = 822/1071 (76.75%), Postives = 933/1071 (87.11%), Query Frame = 0

Query: 115  ISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMA---SGNL 174
            +  ++  +  S +KK RID+         N + ++++   N+I  +   + MA   + N 
Sbjct: 6    VKENIIASASSPMKKRRIDHTESADGSAINASNSSSIGLNNSIGGNDTVMSMAEFGNDNS 65

Query: 175  NPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEG 234
            N Q+IDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG EIAKN+ILAGVKSVTLHDE 
Sbjct: 66   NNQEIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGVEIAKNIILAGVKSVTLHDEN 125

Query: 235  VVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFT 294
            VVELWDLSSNFVF+E DIGKNRALAS  KLQ+LNN+V V TL  KL +EQLS F+VVVF 
Sbjct: 126  VVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLTGKLTKEQLSDFQVVVFV 185

Query: 295  DTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIAS 354
            D   +KA E +D+CH+HQPPI+FIK++VRGLFGS+FCDFGP FTV DV GE+PH+GIIAS
Sbjct: 186  DISFEKATEIDDYCHSHQPPIAFIKADVRGLFGSLFCDFGPHFTVLDVDGEEPHSGIIAS 245

Query: 355  ISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTN 414
            +SN+NP  VSCVDDERLEF+DG+LVVFSEV GMTELNDGKPR+IKN + +SFTL+EDT++
Sbjct: 246  VSNENPGFVSCVDDERLEFEDGNLVVFSEVEGMTELNDGKPRKIKNVKPFSFTLEEDTSS 305

Query: 415  FGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVT 474
            +G Y KGGIVTQVKQPKVLNFKPLREA+ DPGDFLLSDFSKFDRPPL+HLAF ALD+F +
Sbjct: 306  YGQYMKGGIVTQVKQPKVLNFKPLREALKDPGDFLLSDFSKFDRPPLLHLAFQALDRFSS 365

Query: 475  ELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFG 534
            + GRFP AGSE+DAQKL+ +A ++N+ LGD R+ED+N KLLRH AFG++AVLNPMAAMFG
Sbjct: 366  QAGRFPFAGSEEDAQKLVEIAVDINEGLGDARLEDVNSKLLRHLAFGSRAVLNPMAAMFG 425

Query: 535  GIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQK 594
            GIV QEV+KACSGKFHP+ QFFYFDSVESLP E LDASEFRP NSRYDAQISVFGS LQK
Sbjct: 426  GIVGQEVVKACSGKFHPIFQFFYFDSVESLPKEPLDASEFRPQNSRYDAQISVFGSTLQK 485

Query: 595  KLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNI 654
            KLE+A+VF+VG+GALGCEFLKNLALMGVSC  +GKLT+TDDDVIEKSNLSRQFLFRDWNI
Sbjct: 486  KLEDARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQFLFRDWNI 545

Query: 655  GQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYV 714
            GQ KSTVAA+AA  IN  LNI+ALQNRV PETENVF+DSFWENL+V+VNALDNV ARLYV
Sbjct: 546  GQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENLTVVVNALDNVTARLYV 605

Query: 715  DQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC 774
            D RC+YFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC
Sbjct: 606  DSRCVYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC 665

Query: 775  LTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETF 834
            LTWARSEFEGLLEKTP++VNAYLS+P EY  +M  AGDAQ+RDTL RV+ECL++E+C +F
Sbjct: 666  LTWARSEFEGLLEKTPAEVNAYLSDPVEYMKAMRTAGDAQARDTLGRVVECLEKEKCNSF 725

Query: 835  EDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQ 894
            +DCITWARL+FEDYFANRVKQL YTFPEDAATS GAPFWSAPKRFP PL FS  D SH+ 
Sbjct: 726  QDCITWARLRFEDYFANRVKQLCYTFPEDAATSTGAPFWSAPKRFPRPLQFSSTDLSHIN 785

Query: 895  FVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAAS 954
            FV+AA+ILRAE++ I  P+W K    L +AV+RVIVPDF PKKDA IVTDEKAT+LS AS
Sbjct: 786  FVMAASILRAETFGIPTPEWAKTRAGLAEAVERVIVPDFEPKKDATIVTDEKATTLSTAS 845

Query: 955  VDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPE 1014
            VDDAAVI +L  KL   R +L   FRMK IQFEKDDDTNYHMD+IAGLANMRARNYS+PE
Sbjct: 846  VDDAAVIDELNAKLVRCRMSLQPEFRMKAIQFEKDDDTNYHMDMIAGLANMRARNYSVPE 905

Query: 1015 VDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMA 1074
            VDKLKAKFIAGRIIPAIATSTAMATG VCLE+YKVLDG HKVEDYRNTFANLALPLFSMA
Sbjct: 906  VDKLKAKFIAGRIIPAIATSTAMATGFVCLEMYKVLDGSHKVEDYRNTFANLALPLFSMA 965

Query: 1075 EPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPR 1134
            EPVPPKV+KH+D SWTVWDRW+++ NPTLREL+ WLK KGLNAYSISCGS LLYNSMF R
Sbjct: 966  EPVPPKVVKHQDQSWTVWDRWVMRGNPTLRELLDWLKEKGLNAYSISCGSSLLYNSMFSR 1025

Query: 1135 HKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
            HK+RM+++VVDLARD+A VELP YRRH+DVVVACEDD D D+DIPLVSVYF
Sbjct: 1026 HKERMNRRVVDLARDVAGVELPAYRRHVDVVVACEDDNDADVDIPLVSVYF 1076

BLAST of Carg23870-RA vs. ExPASy Swiss-Prot
Match: P20973 (Ubiquitin-activating enzyme E1 1 OS=Triticum aestivum OX=4565 GN=UBA1 PE=1 SV=1)

HSP 1 Score: 1661.7 bits (4302), Expect = 0.0e+00
Identity = 791/1024 (77.25%), Postives = 909/1024 (88.77%), Query Frame = 0

Query: 160  DGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVIL 219
            +G + +   GN    +IDEDLHSRQLAVYGRETM++LF SNVL+SG+QGLGAEIAKN++L
Sbjct: 31   EGDAAMAGRGN----EIDEDLHSRQLAVYGRETMKRLFGSNVLVSGLQGLGAEIAKNLVL 90

Query: 220  AGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVE 279
            AGVKSVTLHD+G VELWDLSSNF  SE+D+G+NRA A  QKLQ+LNN+V+V  L   L +
Sbjct: 91   AGVKSVTLHDDGNVELWDLSSNFFLSENDVGQNRAQACVQKLQELNNAVLVSALTGDLTK 150

Query: 280  EQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDV 339
            E LSKF+ VVFTD  LDKA+EF+D+CH+ QPPI+FIKSEVRGLFGSVFCDFGPEFTV DV
Sbjct: 151  EHLSKFQAVVFTDISLDKAIEFDDYCHSQQPPIAFIKSEVRGLFGSVFCDFGPEFTVLDV 210

Query: 340  YGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCR 399
             GE+PHTGI+ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPR++KN R
Sbjct: 211  DGEEPHTGIVASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRKVKNAR 270

Query: 400  AYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLI 459
             YSF L+EDT++FG Y +GGIVTQVK PKV+ FKPL+EA+++PG+FL+SDFSKF+RPPL+
Sbjct: 271  PYSFFLEEDTSSFGAYVRGGIVTQVKPPKVIKFKPLKEAMSEPGEFLMSDFSKFERPPLL 330

Query: 460  HLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGA 519
            HLAF ALDKF TEL RFPVAGS DD Q++I  A ++N +LGD ++E+I+ KLL HFA G+
Sbjct: 331  HLAFQALDKFRTELSRFPVAGSTDDVQRVIEYAISINDTLGDRKLEEIDKKLLHHFASGS 390

Query: 520  KAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYD 579
            +AVLNPMAAMFGGIV QEV+KACSGKFHPL QFFYFDSVESLP + L+  + +P NSRYD
Sbjct: 391  RAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFDSVESLPVDPLEPGDLKPKNSRYD 450

Query: 580  AQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSN 639
            AQISVFGSKLQ KLE AK+FMVGSGALGCEFLKNLALMG+SCS  G LT+TDDDVIEKSN
Sbjct: 451  AQISVFGSKLQNKLEEAKIFMVGSGALGCEFLKNLALMGISCSQNGNLTLTDDDVIEKSN 510

Query: 640  LSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIV 699
            LSRQFLFRDWNIGQ KSTVAA+AA+ IN  L++EALQNR SPETENVFND+FWENL  +V
Sbjct: 511  LSRQFLFRDWNIGQPKSTVAATAAMVINPKLHVEALQNRASPETENVFNDAFWENLDAVV 570

Query: 700  NALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC 759
            NALDNV AR+Y+D RC+YFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC
Sbjct: 571  NALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC 630

Query: 760  TVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERV 819
            TVHSFPHNIDHCLTWARSEFEGLLEKTP++VNA+LSNP+ Y S+   AGDAQ+RD LERV
Sbjct: 631  TVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAFLSNPTTYISAARTAGDAQARDQLERV 690

Query: 820  LECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHP 879
            +ECLDR++CETF+D ITWARLKFEDYF+NRVKQL +TFPED+ TS+GAPFWSAPKRFP P
Sbjct: 691  IECLDRDKCETFQDSITWARLKFEDYFSNRVKQLTFTFPEDSMTSSGAPFWSAPKRFPRP 750

Query: 880  LPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKL-GDAVDRVIVPDFMPKKDAKI 939
            + FS +DQS L F+LAAAILRAE++ I IP+W K P KL  +AVD+VIVPDF PK+  KI
Sbjct: 751  VEFSSSDQSQLSFILAAAILRAETFGIPIPEWAKTPNKLAAEAVDKVIVPDFQPKQGVKI 810

Query: 940  VTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAG 999
            VT EKATSLS+ASVDDAAVI +L+ KLE+  + LP GF M PIQFEKDDDTN+HMD+IAG
Sbjct: 811  VTHEKATSLSSASVDDAAVIEELIAKLEEVSKTLPSGFHMNPIQFEKDDDTNFHMDVIAG 870

Query: 1000 LANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRN 1059
             ANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK L GGHKVEDYRN
Sbjct: 871  FANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKALAGGHKVEDYRN 930

Query: 1060 TFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSIS 1119
            TFANLA+PLFS+AEPVPPK IKH+++SWTVWDRW +  N TLREL++WLK KGLNAYSIS
Sbjct: 931  TFANLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELLEWLKEKGLNAYSIS 990

Query: 1120 CGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLV 1179
            CG+ LLYNSMFPRHK+R+D+KVVD+AR++AK+E+P YRRHLDVVVACEDD+DND+DIPLV
Sbjct: 991  CGTSLLYNSMFPRHKERLDRKVVDVAREVAKMEVPSYRRHLDVVVACEDDDDNDVDIPLV 1050

Query: 1180 SVYF 1183
            SVYF
Sbjct: 1051 SVYF 1050

BLAST of Carg23870-RA vs. ExPASy Swiss-Prot
Match: P31251 (Ubiquitin-activating enzyme E1 2 OS=Triticum aestivum OX=4565 GN=UBA2 PE=2 SV=1)

HSP 1 Score: 1659.0 bits (4295), Expect = 0.0e+00
Identity = 790/1024 (77.15%), Postives = 907/1024 (88.57%), Query Frame = 0

Query: 160  DGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVIL 219
            +G + +   GN    +IDEDLHSRQLAVYGRETM+ LF SNVL+SG+QGLGAEIAKN++L
Sbjct: 31   EGDAAMAGRGN----EIDEDLHSRQLAVYGRETMKPLFGSNVLVSGLQGLGAEIAKNLVL 90

Query: 220  AGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVE 279
            AGVKSVTLHD+G VELWDLSSNF  SE+D+G+NRA A  QKLQ+LNN+V+V  L   L +
Sbjct: 91   AGVKSVTLHDDGNVELWDLSSNFFLSENDVGQNRAQACVQKLQELNNAVLVSALTGDLTK 150

Query: 280  EQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDV 339
            E LSKF+ VVFTD  LDKA+EF+D+CH+HQPPI+FIKSEVRGLFGSVFCDFGPEFTV DV
Sbjct: 151  EHLSKFQAVVFTDISLDKAIEFDDYCHSHQPPIAFIKSEVRGLFGSVFCDFGPEFTVLDV 210

Query: 340  YGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCR 399
             GE+PHTGI+ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPR++KN R
Sbjct: 211  DGEEPHTGIVASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRKVKNAR 270

Query: 400  AYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLI 459
             YSF L+EDT++FG Y +GGIVTQVK PKV+ FKPL+EA+++PG+FL+SDFSKF+RPPL+
Sbjct: 271  PYSFFLEEDTSSFGAYVRGGIVTQVKPPKVIKFKPLKEAMSEPGEFLMSDFSKFERPPLL 330

Query: 460  HLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGA 519
            HLAF ALDKF TEL RFPVAGS DD Q++I  A ++N +LGD ++E+I+ KLL HFA G+
Sbjct: 331  HLAFQALDKFRTELSRFPVAGSTDDVQRVIEYAISINDTLGDRKLEEIDKKLLHHFASGS 390

Query: 520  KAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYD 579
            +AVLNPMAAMFGGIV QEV+KACSGKFHPL QFFYFDSVESLP + L+  + +P NSRYD
Sbjct: 391  RAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFDSVESLPVDPLEPGDLKPKNSRYD 450

Query: 580  AQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSN 639
            AQISVFGS LQ KLE AK+FMVGSGALGCEFLKNLALMG+SCS  G LT+TDDDVIEKSN
Sbjct: 451  AQISVFGSTLQNKLEEAKIFMVGSGALGCEFLKNLALMGISCSQNGNLTVTDDDVIEKSN 510

Query: 640  LSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIV 699
            LSRQFLFRDWNIGQ KSTVAA+AA+ IN  L++EALQNR SPETENVFND+FWENL  +V
Sbjct: 511  LSRQFLFRDWNIGQPKSTVAATAAMVINPKLHVEALQNRASPETENVFNDAFWENLDAVV 570

Query: 700  NALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC 759
            NALDNV AR+Y+D RC+YFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC
Sbjct: 571  NALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC 630

Query: 760  TVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERV 819
            TVHSFPHNIDHCLTWARSEFEGLLEKTP++VNA+LSNP+ Y S+   AGDAQ+RD LERV
Sbjct: 631  TVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAFLSNPTTYISAARTAGDAQARDQLERV 690

Query: 820  LECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHP 879
            +ECLDR++CETF+D ITWARLKFEDYF+NRVKQL +TFPED+ TS+GAPFWSAPKRFP P
Sbjct: 691  IECLDRDKCETFQDSITWARLKFEDYFSNRVKQLTFTFPEDSMTSSGAPFWSAPKRFPRP 750

Query: 880  LPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKL-GDAVDRVIVPDFMPKKDAKI 939
            + FS +D S L F+LAAAILRAE++ I I +W K P KL  +AVD+VIVPDF PK+  KI
Sbjct: 751  VEFSSSDPSQLSFILAAAILRAETFGIPISEWAKTPNKLAAEAVDKVIVPDFQPKQGVKI 810

Query: 940  VTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAG 999
            VTDEKATSLS+ASVDDAAVI +L+ KLE+  + LP GF M PIQFEKDDDTN+HMD+IAG
Sbjct: 811  VTDEKATSLSSASVDDAAVIEELIAKLEEVSKTLPSGFHMNPIQFEKDDDTNFHMDVIAG 870

Query: 1000 LANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRN 1059
             ANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK L GGHKVEDYRN
Sbjct: 871  FANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKALAGGHKVEDYRN 930

Query: 1060 TFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSIS 1119
            TFANLA+PLFS+AEPVPPK IKH+++SWTVWDRW +  N TLREL++WLK KGLNAYSIS
Sbjct: 931  TFANLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELLEWLKEKGLNAYSIS 990

Query: 1120 CGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLV 1179
            CG+ LLYNSMFPRHK+R+D+KVVD+AR++AK+E+P YRRHLDVVVACEDD+DND+DIPLV
Sbjct: 991  CGTSLLYNSMFPRHKERLDRKVVDVAREVAKMEVPSYRRHLDVVVACEDDDDNDVDIPLV 1050

Query: 1180 SVYF 1183
            SVYF
Sbjct: 1051 SVYF 1050

BLAST of Carg23870-RA vs. ExPASy Swiss-Prot
Match: P31252 (Ubiquitin-activating enzyme E1 3 OS=Triticum aestivum OX=4565 GN=UBA3 PE=1 SV=1)

HSP 1 Score: 1597.0 bits (4134), Expect = 0.0e+00
Identity = 756/1010 (74.85%), Postives = 886/1010 (87.72%), Query Frame = 0

Query: 173  PQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGV 232
            PQ+IDEDLHSRQLAVYGRETMR+LFAS+VL+SG+ GLGAEIAKN+ LAGVKSVT+HD   
Sbjct: 43   PQEIDEDLHSRQLAVYGRETMRRLFASDVLVSGLNGLGAEIAKNLALAGVKSVTIHDVKT 102

Query: 233  VELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTD 292
            V++WDLS NF  SE DIGKNRA A   KLQ+LNN+V++  L  +L  E LSKF+ VVFTD
Sbjct: 103  VKMWDLSGNFFLSEDDIGKNRAAACVAKLQELNNAVLISALTEELTTEHLSKFQAVVFTD 162

Query: 293  TGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI 352
              LDKA EF+D+CHNHQPPISFIKSEV GLFGSVFCDFGP+FTV DV GEDPHTGIIASI
Sbjct: 163  IDLDKAYEFDDYCHNHQPPISFIKSEVCGLFGSVFCDFGPKFTVLDVDGEDPHTGIIASI 222

Query: 353  SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNF 412
            SNDNPAL+SCVDDERLEFQDGDLVVFSEVHGMTELNDGKPR++KN R +SF+++EDT+NF
Sbjct: 223  SNDNPALISCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRKVKNARPFSFSIEEDTSNF 282

Query: 413  GIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTE 472
            GIY KGGIVTQVK+PKVL FK LR+A+ DPG+ LLSDFSKF+RPP++HLAF ALDKF  +
Sbjct: 283  GIYVKGGIVTQVKEPKVLCFKALRDAMTDPGEVLLSDFSKFERPPVLHLAFQALDKFKKD 342

Query: 473  LGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG 532
             GR P AG E+DA   + +A+ +N++  D +++ I+ KL R FA G++AVLNPMAAMFGG
Sbjct: 343  HGRCPAAGCEEDAHSFLKIAAAINEASADRKLDTIDEKLFRQFASGSRAVLNPMAAMFGG 402

Query: 533  IVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKK 592
            IV QEV+KACSGKFHPL QFFYFDSVESLPT  L+  + +P N+RYDAQ+SVFGSKLQKK
Sbjct: 403  IVGQEVVKACSGKFHPLNQFFYFDSVESLPTYPLEPQDLKPSNNRYDAQVSVFGSKLQKK 462

Query: 593  LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG 652
            +E A  F+VGSGALGCEFLKNLALMGVSCS++GKLTITDDD+IEKSNLSRQFLFRDWNIG
Sbjct: 463  MEEANTFVVGSGALGCEFLKNLALMGVSCSSKGKLTITDDDIIEKSNLSRQFLFRDWNIG 522

Query: 653  QNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVD 712
            Q KSTVAA+AA AIN  L+I+ALQNR  P+TENVF+D+FWE L V++NALDNVNAR+Y+D
Sbjct: 523  QAKSTVAATAASAINPSLHIDALQNRACPDTENVFHDTFWEGLDVVINALDNVNARMYMD 582

Query: 713  QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 772
             RCLYFQKPLLESGTLGAKCN QMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL
Sbjct: 583  MRCLYFQKPLLESGTLGAKCNIQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 642

Query: 773  TWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFE 832
            TWARSEFEGLLEKTP++VN++LSNP++YA++M  AGDAQ+R+ LERV ECL+++RC TF+
Sbjct: 643  TWARSEFEGLLEKTPNEVNSFLSNPAQYAAAMRKAGDAQARELLERVSECLNKDRCSTFD 702

Query: 833  DCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQF 892
            DCI+WARLKFEDYF+NRVKQL +TFPEDAATS GAPFWSAPKRFP  L FS ADQSHL F
Sbjct: 703  DCISWARLKFEDYFSNRVKQLTFTFPEDAATSMGAPFWSAPKRFPRALQFSAADQSHLNF 762

Query: 893  VLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASV 952
            +++A+ILRAES+ ++IP+W K+  KL D V+++ VP F PK+   IVTDEKA++LS+ SV
Sbjct: 763  IMSASILRAESFGVAIPEWAKDTSKLADVVNKIAVPTFEPKQGVNIVTDEKASNLSSTSV 822

Query: 953  DDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEV 1012
            DD AVI DL+ KL++  + L  GF+MKPIQFEKDDDTN+HMDLI+GLANMRARNYSIPEV
Sbjct: 823  DDVAVIEDLLAKLQEYAKMLLPGFQMKPIQFEKDDDTNFHMDLISGLANMRARNYSIPEV 882

Query: 1013 DKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAE 1072
            DKLKAKFIAGRIIPAIATSTAMATGLVCLELYKV+ G H VEDYRNTFANLALPLFSMAE
Sbjct: 883  DKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVIAGEHPVEDYRNTFANLALPLFSMAE 942

Query: 1073 PVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRH 1132
            PVPPKV+KH++ SWTVWDRW ++ N TL EL++W  +KGL AYSISCG+ LLYN+MF RH
Sbjct: 943  PVPPKVMKHKETSWTVWDRWSVQGNLTLAELLQWFADKGLTAYSISCGTSLLYNNMFARH 1002

Query: 1133 KDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
            KDR+ KKVVD+AR++AKV++P YRRHLD+ VACED+++ND+DIPLVSVYF
Sbjct: 1003 KDRLTKKVVDIAREVAKVDVPEYRRHLDIGVACEDEDENDVDIPLVSVYF 1052

BLAST of Carg23870-RA vs. ExPASy TrEMBL
Match: A0A6J1G6A0 (E1 ubiquitin-activating enzyme OS=Cucurbita moschata OX=3662 GN=LOC111451218 PE=3 SV=1)

HSP 1 Score: 2200.2 bits (5700), Expect = 0.0e+00
Identity = 1092/1092 (100.00%), Postives = 1092/1092 (100.00%), Query Frame = 0

Query: 91   MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 150
            MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA
Sbjct: 1    MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 60

Query: 151  VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 210
            VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG
Sbjct: 61   VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 120

Query: 211  AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 270
            AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV
Sbjct: 121  AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 180

Query: 271  HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 330
            HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF
Sbjct: 181  HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 240

Query: 331  GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 390
            GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG
Sbjct: 241  GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 300

Query: 391  KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 450
            KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF
Sbjct: 301  KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 360

Query: 451  SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK 510
            SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK
Sbjct: 361  SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK 420

Query: 511  LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 570
            LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE
Sbjct: 421  LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 480

Query: 571  FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 630
            FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT
Sbjct: 481  FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 540

Query: 631  DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 690
            DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS
Sbjct: 541  DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 600

Query: 691  FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 750
            FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD
Sbjct: 601  FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 660

Query: 751  PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 810
            PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA
Sbjct: 661  PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 720

Query: 811  QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 870
            QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW
Sbjct: 721  QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 780

Query: 871  SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF 930
            SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF
Sbjct: 781  SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF 840

Query: 931  MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 990
            MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN
Sbjct: 841  MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 900

Query: 991  YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 1050
            YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG
Sbjct: 901  YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 960

Query: 1051 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1110
            HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK
Sbjct: 961  HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1020

Query: 1111 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1170
            GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED
Sbjct: 1021 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1080

Query: 1171 NDIDIPLVSVYF 1183
            NDIDIPLVSVYF
Sbjct: 1081 NDIDIPLVSVYF 1092

BLAST of Carg23870-RA vs. ExPASy TrEMBL
Match: A0A6J1KZ04 (E1 ubiquitin-activating enzyme OS=Cucurbita maxima OX=3661 GN=LOC111499539 PE=3 SV=1)

HSP 1 Score: 2172.1 bits (5627), Expect = 0.0e+00
Identity = 1081/1095 (98.72%), Postives = 1087/1095 (99.27%), Query Frame = 0

Query: 91   MLPRKRAGEEGAVLEQQIDNSS---SNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNV 150
            MLPRKRAGEEGAVLEQQIDNSS   SNISNSVQNAGVSLIKKHRIDNCNVDSNVN NTNV
Sbjct: 1    MLPRKRAGEEGAVLEQQIDNSSSNISNISNSVQNAGVSLIKKHRIDNCNVDSNVNFNTNV 60

Query: 151  AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 210
            AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ
Sbjct: 61   AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 120

Query: 211  GLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS 270
            GLGAEIAKNVILAGVKSVTLHD+GVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS
Sbjct: 121  GLGAEIAKNVILAGVKSVTLHDDGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS 180

Query: 271  VIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF 330
            VIVHTL TKLV+EQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF
Sbjct: 181  VIVHTLTTKLVKEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF 240

Query: 331  CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL 390
            CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL
Sbjct: 241  CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL 300

Query: 391  NDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL 450
            NDGKPRRIKNCRAYSFTLDEDTTN+GIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL
Sbjct: 301  NDGKPRRIKNCRAYSFTLDEDTTNYGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL 360

Query: 451  SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDI 510
            SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMN+SLGDGRVEDI
Sbjct: 361  SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNESLGDGRVEDI 420

Query: 511  NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD 570
            NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD
Sbjct: 421  NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD 480

Query: 571  ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL 630
            ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL
Sbjct: 481  ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL 540

Query: 631  TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF 690
            TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF
Sbjct: 541  TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF 600

Query: 691  NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA 750
            NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA
Sbjct: 601  NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA 660

Query: 751  SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA 810
            SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA
Sbjct: 661  SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA 720

Query: 811  GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA 870
            GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA
Sbjct: 721  GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA 780

Query: 871  PFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIV 930
            PFWSAPKRFPHPLPFS ADQSHLQFVLAAAILRAESYAISIPDWVKNPRKL DAVDRVIV
Sbjct: 781  PFWSAPKRFPHPLPFSTADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLADAVDRVIV 840

Query: 931  PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDD 990
            PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLV+KLEDTRRNLPEGFRMKPIQFEKDD
Sbjct: 841  PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVDKLEDTRRNLPEGFRMKPIQFEKDD 900

Query: 991  DTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL 1050
            DTNYHMDLIAGLANMRARN+SIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL
Sbjct: 901  DTNYHMDLIAGLANMRARNFSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL 960

Query: 1051 DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL 1110
            DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL
Sbjct: 961  DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL 1020

Query: 1111 KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACED 1170
            KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPY RHLDVVVACED
Sbjct: 1021 KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYCRHLDVVVACED 1080

Query: 1171 DEDNDIDIPLVSVYF 1183
            DEDNDIDIPLVSVYF
Sbjct: 1081 DEDNDIDIPLVSVYF 1095

BLAST of Carg23870-RA vs. ExPASy TrEMBL
Match: A0A0A0KE12 (E1 ubiquitin-activating enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G181590 PE=3 SV=1)

HSP 1 Score: 2069.7 bits (5361), Expect = 0.0e+00
Identity = 1040/1157 (89.89%), Postives = 1076/1157 (93.00%), Query Frame = 0

Query: 48   FHSSSKKPLHSSSILSF----------HSPSPDSFLPFFL------SLCYAPNRSLLHFM 107
            F SSS     SSSI  F          H P   SF P F+      S       SLLHFM
Sbjct: 7    FLSSSSSSSTSSSISPFLFFEKPLDSHHIPHCSSFNPPFIRFLPSSSFTVLIGTSLLHFM 66

Query: 108  LPRKRAGEEGAVLEQQIDNSSSNI-----SNSVQNAGVSLIKKHRIDNCNVDSNVNSNTN 167
            LPRKRAGEEG V+E++ DN S+++     ++SVQN G S IKK RID+   DSN N+N  
Sbjct: 67   LPRKRAGEEGVVVEEETDNGSTSVVINNNNSSVQNVGASFIKKQRIDS---DSNSNTNVA 126

Query: 168  VAAAVP-TGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISG 227
              A VP T +NIVND ASLIMASGN NP DIDEDLHSRQLAVYGRETMRKLFASNVLISG
Sbjct: 127  AVATVPTTASNIVNDAASLIMASGNSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG 186

Query: 228  MQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLN 287
            MQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESD+GKNRALASAQKLQDLN
Sbjct: 187  MQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDVGKNRALASAQKLQDLN 246

Query: 288  NSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGS 347
            NSVIVHTL +KLV+EQLS FEVVVFTDT LDKAMEFNDFCHNHQPPISFIKSEVRGLFGS
Sbjct: 247  NSVIVHTLTSKLVKEQLSDFEVVVFTDTSLDKAMEFNDFCHNHQPPISFIKSEVRGLFGS 306

Query: 348  VFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMT 407
            VFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMT
Sbjct: 307  VFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMT 366

Query: 408  ELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDF 467
            ELNDGKPRRIKNCRAYSFTL+EDTTNFG YEKGGIVTQVKQPKVLNFKPLREAINDPGDF
Sbjct: 367  ELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKQPKVLNFKPLREAINDPGDF 426

Query: 468  LLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVE 527
            LLSDFSKFDRPPLIHLAFLALDKFVTELGR PVAGSE+DAQKLISVASN+N+SLGDGRVE
Sbjct: 427  LLSDFSKFDRPPLIHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNINESLGDGRVE 486

Query: 528  DINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEA 587
            DINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTE+
Sbjct: 487  DINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTES 546

Query: 588  LDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEG 647
            LDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCS+EG
Sbjct: 547  LDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSSEG 606

Query: 648  KLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETEN 707
            KLTITDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AAVAINRHLNIEALQNRVSPETEN
Sbjct: 607  KLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAAAAAVAINRHLNIEALQNRVSPETEN 666

Query: 708  VFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENY 767
            VF+DSFWENLSV+VNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENY
Sbjct: 667  VFDDSFWENLSVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENY 726

Query: 768  GASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMM 827
            GASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP+DVNAYLSNPSEY S+MM
Sbjct: 727  GASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMM 786

Query: 828  NAGDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSN 887
            NAGDAQSRDTLER+LECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDA TSN
Sbjct: 787  NAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAQTSN 846

Query: 888  GAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRV 947
            GAPFWSAPKRFPHPL FS +DQSHLQFVLAAAILR+ESYAI IPDWVKNPRKL DA+DR+
Sbjct: 847  GAPFWSAPKRFPHPLQFSTSDQSHLQFVLAAAILRSESYAIPIPDWVKNPRKLADAIDRI 906

Query: 948  IVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEK 1007
            IVPDFMPKKDAKIVTDEKATSLS ASVDDAAVIHDL  KLE+T R LPEGFRMKPIQFEK
Sbjct: 907  IVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLAGKLEETCRKLPEGFRMKPIQFEK 966

Query: 1008 DDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK 1067
            DDD+N+HMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK
Sbjct: 967  DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK 1026

Query: 1068 VLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIK 1127
            VLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI 
Sbjct: 1027 VLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELID 1086

Query: 1128 WLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVAC 1183
            WLKNKGLNAYSISCGSCLLYNSMFPRH+DRMDKKVVDLARD+AKVELPPYRRHLDVVVAC
Sbjct: 1087 WLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVAC 1146

BLAST of Carg23870-RA vs. ExPASy TrEMBL
Match: A0A5A7VQ32 (E1 ubiquitin-activating enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold615G00120 PE=3 SV=1)

HSP 1 Score: 2068.9 bits (5359), Expect = 0.0e+00
Identity = 1025/1117 (91.76%), Postives = 1064/1117 (95.26%), Query Frame = 0

Query: 72   FLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQIDNSSSNI-----SNSVQNAGVSL 131
            F+ F +  C+    SLLHFMLPRKRAGEEG V+E++ DN S+++     ++SVQN G SL
Sbjct: 38   FVSFLILRCFGIFSSLLHFMLPRKRAGEEGVVVEEETDNGSTSVVFNNNNSSVQNVGASL 97

Query: 132  IKKHRIDNCNVDSNVNSNTNVAAAVP-TGNNIVNDGASLIMASGNLNPQDIDEDLHSRQL 191
            IKK RID+   DSN N+N    A VP T NNIVND ASLIMASGN NP DIDEDLHSRQL
Sbjct: 98   IKKQRIDS---DSNSNTNVAAVATVPTTANNIVNDAASLIMASGNSNPPDIDEDLHSRQL 157

Query: 192  AVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFS 251
            AVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFS
Sbjct: 158  AVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFS 217

Query: 252  ESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFC 311
            ESD+GKNRALASAQKLQDLNNSVIVHTL TKLV+EQLS FEVVVFTDT LDKAMEF+DFC
Sbjct: 218  ESDVGKNRALASAQKLQDLNNSVIVHTLTTKLVKEQLSDFEVVVFTDTSLDKAMEFDDFC 277

Query: 312  HNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDD 371
            HNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDD
Sbjct: 278  HNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDD 337

Query: 372  ERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVK 431
            ERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTL+EDT NFG YEKGGIVTQVK
Sbjct: 338  ERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTANFGSYEKGGIVTQVK 397

Query: 432  QPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDA 491
            QPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGR P+AGSE+DA
Sbjct: 398  QPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRLPIAGSEEDA 457

Query: 492  QKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGK 551
            QKLISVAS +N+SLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGK
Sbjct: 458  QKLISVASTINESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGK 517

Query: 552  FHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGA 611
            FHPLVQFFYFDSVESLPTE+LDAS+FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGA
Sbjct: 518  FHPLVQFFYFDSVESLPTESLDASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGA 577

Query: 612  LGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVA 671
            LGCEFLKNLALMGVSCS+EGKLTITDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AAVA
Sbjct: 578  LGCEFLKNLALMGVSCSSEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAAAAAVA 637

Query: 672  INRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLES 731
            INRHLNIEALQNRVSPETENVF+DSFWENLSV+VNALDNVNARLYVDQRCLYFQKPLLES
Sbjct: 638  INRHLNIEALQNRVSPETENVFDDSFWENLSVVVNALDNVNARLYVDQRCLYFQKPLLES 697

Query: 732  GTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEK 791
            GTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEK
Sbjct: 698  GTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEK 757

Query: 792  TPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDY 851
            TP+DVNAYLSNP+EY S+MMNAGDAQSRDTLER+LECLDRERCETFEDCITWARLKFEDY
Sbjct: 758  TPTDVNAYLSNPNEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDY 817

Query: 852  FANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYA 911
            FANRVKQLIYTFPEDA TSNGAPFWSAPKRFPHPL FS +DQSHLQFVLAAAILRAESYA
Sbjct: 818  FANRVKQLIYTFPEDAQTSNGAPFWSAPKRFPHPLQFSTSDQSHLQFVLAAAILRAESYA 877

Query: 912  ISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKL 971
            I +PDWVKNPRKL DA+DRVIVPDFMPKKDAKIVTDEKATSLS ASVDDAAVIHDL  KL
Sbjct: 878  IPVPDWVKNPRKLADAIDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLAGKL 937

Query: 972  EDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRII 1031
            E+TRR LPEGFRMKPIQFEKDDD+N+HMDLIAGLANMRARNYSIPEVDKLKAKFIAGRII
Sbjct: 938  EETRRKLPEGFRMKPIQFEKDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRII 997

Query: 1032 PAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMS 1091
            PAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMS
Sbjct: 998  PAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMS 1057

Query: 1092 WTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLAR 1151
            WTVWDRWIIKDNPTLRELI WLKNKGLNAYSISCGSCLLYNSMFPRH+DRMDKKVVDLAR
Sbjct: 1058 WTVWDRWIIKDNPTLRELIDWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLAR 1117

Query: 1152 DIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
            D+AKVELP YRRHLDVVVACEDDEDNDIDIPLVSVYF
Sbjct: 1118 DVAKVELPLYRRHLDVVVACEDDEDNDIDIPLVSVYF 1151

BLAST of Carg23870-RA vs. ExPASy TrEMBL
Match: A0A1S3CNI4 (E1 ubiquitin-activating enzyme OS=Cucumis melo OX=3656 GN=LOC103502928 PE=3 SV=1)

HSP 1 Score: 2054.3 bits (5321), Expect = 0.0e+00
Identity = 1017/1098 (92.62%), Postives = 1053/1098 (95.90%), Query Frame = 0

Query: 91   MLPRKRAGEEGAVLEQQIDNSSSNI-----SNSVQNAGVSLIKKHRIDNCNVDSNVNSNT 150
            MLPRKRAGEEG V+E++ DN S+++     ++SVQN G SLIKK RID+   DSN N+N 
Sbjct: 1    MLPRKRAGEEGVVVEEETDNGSTSVVFNNNNSSVQNVGASLIKKQRIDS---DSNSNTNV 60

Query: 151  NVAAAVP-TGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLIS 210
               A VP T NNIVND ASLIMASGN NP DIDEDLHSRQLAVYGRETMRKLFASNVLIS
Sbjct: 61   AAVATVPTTANNIVNDAASLIMASGNSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLIS 120

Query: 211  GMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDL 270
            GMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESD+GKNRALASAQKLQDL
Sbjct: 121  GMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDVGKNRALASAQKLQDL 180

Query: 271  NNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFG 330
            NNSVIVHTL TKLV+EQLS FEVVVFTDT LDKAMEF+DFCHNHQPPISFIKSEVRGLFG
Sbjct: 181  NNSVIVHTLTTKLVKEQLSDFEVVVFTDTSLDKAMEFDDFCHNHQPPISFIKSEVRGLFG 240

Query: 331  SVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGM 390
            SVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGM
Sbjct: 241  SVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGM 300

Query: 391  TELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGD 450
            TELNDGKPRRIKNCRAYSFTL+EDT NFG YEKGGIVTQVKQPKVLNFKPLREAINDPGD
Sbjct: 301  TELNDGKPRRIKNCRAYSFTLEEDTANFGSYEKGGIVTQVKQPKVLNFKPLREAINDPGD 360

Query: 451  FLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRV 510
            FLLSDFSKFDRPPLIHLAFLALDKFVTELGR P+AGSE+DAQKLISVAS +N+SLGDGRV
Sbjct: 361  FLLSDFSKFDRPPLIHLAFLALDKFVTELGRLPIAGSEEDAQKLISVASTINESLGDGRV 420

Query: 511  EDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTE 570
            EDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTE
Sbjct: 421  EDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTE 480

Query: 571  ALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNE 630
            +LDAS+FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCS+E
Sbjct: 481  SLDASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSSE 540

Query: 631  GKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETE 690
            GKLTITDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AAVAINRHLNIEALQNRVSPETE
Sbjct: 541  GKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAAAAAVAINRHLNIEALQNRVSPETE 600

Query: 691  NVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTEN 750
            NVF+DSFWENLSV+VNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTEN
Sbjct: 601  NVFDDSFWENLSVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTEN 660

Query: 751  YGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSM 810
            YGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP+DVNAYLSNP+EY S+M
Sbjct: 661  YGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPNEYTSAM 720

Query: 811  MNAGDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATS 870
            MNAGDAQSRDTLER+LECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDA TS
Sbjct: 721  MNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAQTS 780

Query: 871  NGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDR 930
            NGAPFWSAPKRFPHPL FS +DQSHLQFVLAAAILRAESYAI +PDWVKNPRKL DA+DR
Sbjct: 781  NGAPFWSAPKRFPHPLQFSTSDQSHLQFVLAAAILRAESYAIPVPDWVKNPRKLADAIDR 840

Query: 931  VIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFE 990
            VIVPDFMPKKDAKIVTDEKATSLS ASVDDAAVIHDL  KLE+TRR LPEGFRMKPIQFE
Sbjct: 841  VIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLAGKLEETRRKLPEGFRMKPIQFE 900

Query: 991  KDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELY 1050
            KDDD+N+HMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELY
Sbjct: 901  KDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELY 960

Query: 1051 KVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI 1110
            KVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI
Sbjct: 961  KVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI 1020

Query: 1111 KWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVA 1170
             WLKNKGLNAYSISCGSCLLYNSMFPRH+DRMDKKVVDLARD+AKVELP YRRHLDVVVA
Sbjct: 1021 DWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPLYRRHLDVVVA 1080

Query: 1171 CEDDEDNDIDIPLVSVYF 1183
            CEDDEDNDIDIPLVSVYF
Sbjct: 1081 CEDDEDNDIDIPLVSVYF 1095

BLAST of Carg23870-RA vs. TAIR 10
Match: AT2G30110.1 (ubiquitin-activating enzyme 1 )

HSP 1 Score: 1726.5 bits (4470), Expect = 0.0e+00
Identity = 834/1080 (77.22%), Postives = 956/1080 (88.52%), Query Frame = 0

Query: 103  VLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGA 162
            +L ++   ++    N++  + ++  KK RID     S+  S++ +A+    G +  +   
Sbjct: 1    MLHKRASEANDKNDNTIIGSDLASSKKRRIDFTESSSD-KSSSILASGSSRGFHGDSVVQ 60

Query: 163  SLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGV 222
             + MA GN N Q+IDEDLHSRQLAVYGRETMR+LFASNVLISGM GLGAEIAKN+ILAGV
Sbjct: 61   QIDMAFGNSNRQEIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEIAKNLILAGV 120

Query: 223  KSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQL 282
            KSVTLHDE VVELWDLSSNFVFSE D+GKNRA AS QKLQDLNN+V+V +L   L +E L
Sbjct: 121  KSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSSLTKSLNKEDL 180

Query: 283  SKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGE 342
            S F+VVVF+D  +++A+EF+D+CH+HQPPI+F+K++VRGLFGSVFCDFGPEF V DV GE
Sbjct: 181  SGFQVVVFSDISMERAIEFDDYCHSHQPPIAFVKADVRGLFGSVFCDFGPEFAVLDVDGE 240

Query: 343  DPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYS 402
            +PHTGIIASISN+N A +SCVDDERLEF+DGDLVVFSEV GMTELNDGKPR+IK+ R YS
Sbjct: 241  EPHTGIIASISNENQAFISCVDDERLEFEDGDLVVFSEVEGMTELNDGKPRKIKSTRPYS 300

Query: 403  FTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLA 462
            FTLDEDTTN+G Y KGGIVTQVKQPK+LNFKPLREA+ DPGDFL SDFSKFDRPPL+HLA
Sbjct: 301  FTLDEDTTNYGTYVKGGIVTQVKQPKLLNFKPLREALKDPGDFLFSDFSKFDRPPLLHLA 360

Query: 463  FLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAV 522
            F ALD F  E GRFPVAGSE+DAQKLIS+A+ +N   GD +VE+++ KLLRHF+FGAKAV
Sbjct: 361  FQALDHFKAEAGRFPVAGSEEDAQKLISIATAINTGQGDLKVENVDQKLLRHFSFGAKAV 420

Query: 523  LNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQI 582
            LNPMAAMFGGIV QEV+KACSGKFHPL QFFYFDSVESLP+E +D+S+F P NSRYDAQI
Sbjct: 421  LNPMAAMFGGIVGQEVVKACSGKFHPLFQFFYFDSVESLPSEPVDSSDFAPRNSRYDAQI 480

Query: 583  SVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSR 642
            SVFG+K QKKLE+AKVF VGSGALGCEFLKNLALMGVSC ++GKLT+TDDD+IEKSNLSR
Sbjct: 481  SVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGVSCGSQGKLTVTDDDIIEKSNLSR 540

Query: 643  QFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNAL 702
            QFLFRDWNIGQ KSTVAASAA  IN   NIEALQNRV  ETENVF+D+FWENL+V+VNAL
Sbjct: 541  QFLFRDWNIGQAKSTVAASAAAVINPRFNIEALQNRVGAETENVFDDAFWENLTVVVNAL 600

Query: 703  DNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVH 762
            DNVNARLYVD RCLYFQKPLLESGTLG KCNTQ VIPHLTENYGASRDPPEKQAPMCTVH
Sbjct: 601  DNVNARLYVDSRCLYFQKPLLESGTLGTKCNTQSVIPHLTENYGASRDPPEKQAPMCTVH 660

Query: 763  SFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLEC 822
            SFPHNIDHCLTWARSEFEGLLEKTP++VNAYLS+P EY +SMM+AGDAQ+RDTLER++EC
Sbjct: 661  SFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSSPVEYTNSMMSAGDAQARDTLERIVEC 720

Query: 823  LDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPF 882
            L++E+CETF+DC+TWARL+FEDYF NRVKQLIYTFPEDAATS GAPFWSAPKRFP PL +
Sbjct: 721  LEKEKCETFQDCLTWARLRFEDYFVNRVKQLIYTFPEDAATSTGAPFWSAPKRFPRPLQY 780

Query: 883  SPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDE 942
            S +D S L F+ A AILRAE++ I IP+W KNP++  +AVDRVIVPDF P++DAKIVTDE
Sbjct: 781  SSSDPSLLNFITATAILRAETFGIPIPEWTKNPKEAAEAVDRVIVPDFEPRQDAKIVTDE 840

Query: 943  KATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANM 1002
            KAT+L+ ASVDDAAVI DL+ K++  R NL   FRMKPIQFEKDDDTNYHMD+IAGLANM
Sbjct: 841  KATTLTTASVDDAAVIDDLIAKIDQCRHNLSPDFRMKPIQFEKDDDTNYHMDVIAGLANM 900

Query: 1003 RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFAN 1062
            RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVE YRNTFAN
Sbjct: 901  RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEAYRNTFAN 960

Query: 1063 LALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSC 1122
            LALPLFSMAEP+PPKV+KHRDM+WTVWDRW++K NPTLRE+++WL++KGL+AYSISCGSC
Sbjct: 961  LALPLFSMAEPLPPKVVKHRDMAWTVWDRWVLKGNPTLREVLQWLEDKGLSAYSISCGSC 1020

Query: 1123 LLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1182
            LL+NSMF RHK+RMDKKVVDLARD+AKVELPPYR HLDVVVACED++DND+DIPLVS+YF
Sbjct: 1021 LLFNSMFTRHKERMDKKVVDLARDVAKVELPPYRNHLDVVVACEDEDDNDVDIPLVSIYF 1079

BLAST of Carg23870-RA vs. TAIR 10
Match: AT5G06460.1 (ubiquitin activating enzyme 2 )

HSP 1 Score: 1694.1 bits (4386), Expect = 0.0e+00
Identity = 822/1071 (76.75%), Postives = 933/1071 (87.11%), Query Frame = 0

Query: 115  ISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMA---SGNL 174
            +  ++  +  S +KK RID+         N + ++++   N+I  +   + MA   + N 
Sbjct: 6    VKENIIASASSPMKKRRIDHTESADGSAINASNSSSIGLNNSIGGNDTVMSMAEFGNDNS 65

Query: 175  NPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEG 234
            N Q+IDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG EIAKN+ILAGVKSVTLHDE 
Sbjct: 66   NNQEIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGVEIAKNIILAGVKSVTLHDEN 125

Query: 235  VVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFT 294
            VVELWDLSSNFVF+E DIGKNRALAS  KLQ+LNN+V V TL  KL +EQLS F+VVVF 
Sbjct: 126  VVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLTGKLTKEQLSDFQVVVFV 185

Query: 295  DTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIAS 354
            D   +KA E +D+CH+HQPPI+FIK++VRGLFGS+FCDFGP FTV DV GE+PH+GIIAS
Sbjct: 186  DISFEKATEIDDYCHSHQPPIAFIKADVRGLFGSLFCDFGPHFTVLDVDGEEPHSGIIAS 245

Query: 355  ISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTN 414
            +SN+NP  VSCVDDERLEF+DG+LVVFSEV GMTELNDGKPR+IKN + +SFTL+EDT++
Sbjct: 246  VSNENPGFVSCVDDERLEFEDGNLVVFSEVEGMTELNDGKPRKIKNVKPFSFTLEEDTSS 305

Query: 415  FGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVT 474
            +G Y KGGIVTQVKQPKVLNFKPLREA+ DPGDFLLSDFSKFDRPPL+HLAF ALD+F +
Sbjct: 306  YGQYMKGGIVTQVKQPKVLNFKPLREALKDPGDFLLSDFSKFDRPPLLHLAFQALDRFSS 365

Query: 475  ELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFG 534
            + GRFP AGSE+DAQKL+ +A ++N+ LGD R+ED+N KLLRH AFG++AVLNPMAAMFG
Sbjct: 366  QAGRFPFAGSEEDAQKLVEIAVDINEGLGDARLEDVNSKLLRHLAFGSRAVLNPMAAMFG 425

Query: 535  GIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQK 594
            GIV QEV+KACSGKFHP+ QFFYFDSVESLP E LDASEFRP NSRYDAQISVFGS LQK
Sbjct: 426  GIVGQEVVKACSGKFHPIFQFFYFDSVESLPKEPLDASEFRPQNSRYDAQISVFGSTLQK 485

Query: 595  KLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNI 654
            KLE+A+VF+VG+GALGCEFLKNLALMGVSC  +GKLT+TDDDVIEKSNLSRQFLFRDWNI
Sbjct: 486  KLEDARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQFLFRDWNI 545

Query: 655  GQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYV 714
            GQ KSTVAA+AA  IN  LNI+ALQNRV PETENVF+DSFWENL+V+VNALDNV ARLYV
Sbjct: 546  GQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENLTVVVNALDNVTARLYV 605

Query: 715  DQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC 774
            D RC+YFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC
Sbjct: 606  DSRCVYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC 665

Query: 775  LTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETF 834
            LTWARSEFEGLLEKTP++VNAYLS+P EY  +M  AGDAQ+RDTL RV+ECL++E+C +F
Sbjct: 666  LTWARSEFEGLLEKTPAEVNAYLSDPVEYMKAMRTAGDAQARDTLGRVVECLEKEKCNSF 725

Query: 835  EDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQ 894
            +DCITWARL+FEDYFANRVKQL YTFPEDAATS GAPFWSAPKRFP PL FS  D SH+ 
Sbjct: 726  QDCITWARLRFEDYFANRVKQLCYTFPEDAATSTGAPFWSAPKRFPRPLQFSSTDLSHIN 785

Query: 895  FVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAAS 954
            FV+AA+ILRAE++ I  P+W K    L +AV+RVIVPDF PKKDA IVTDEKAT+LS AS
Sbjct: 786  FVMAASILRAETFGIPTPEWAKTRAGLAEAVERVIVPDFEPKKDATIVTDEKATTLSTAS 845

Query: 955  VDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPE 1014
            VDDAAVI +L  KL   R +L   FRMK IQFEKDDDTNYHMD+IAGLANMRARNYS+PE
Sbjct: 846  VDDAAVIDELNAKLVRCRMSLQPEFRMKAIQFEKDDDTNYHMDMIAGLANMRARNYSVPE 905

Query: 1015 VDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMA 1074
            VDKLKAKFIAGRIIPAIATSTAMATG VCLE+YKVLDG HKVEDYRNTFANLALPLFSMA
Sbjct: 906  VDKLKAKFIAGRIIPAIATSTAMATGFVCLEMYKVLDGSHKVEDYRNTFANLALPLFSMA 965

Query: 1075 EPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPR 1134
            EPVPPKV+KH+D SWTVWDRW+++ NPTLREL+ WLK KGLNAYSISCGS LLYNSMF R
Sbjct: 966  EPVPPKVVKHQDQSWTVWDRWVMRGNPTLRELLDWLKEKGLNAYSISCGSSLLYNSMFSR 1025

Query: 1135 HKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
            HK+RM+++VVDLARD+A VELP YRRH+DVVVACEDD D D+DIPLVSVYF
Sbjct: 1026 HKERMNRRVVDLARDVAGVELPAYRRHVDVVVACEDDNDADVDIPLVSVYF 1076

BLAST of Carg23870-RA vs. TAIR 10
Match: AT2G21470.3 (SUMO-activating enzyme 2 )

HSP 1 Score: 164.9 bits (416), Expect = 4.1e-40
Identity = 161/552 (29.17%), Postives = 244/552 (44.20%), Query Frame = 0

Query: 590  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 649
            Q  ++ AKV MVG+G +GCE LK LAL G        + I D D IE SNL+RQFLFR  
Sbjct: 7    QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66

Query: 650  NIGQNKSTVAASAAVAINRHLNIEALQNRV-SPETENVFNDSFWENLSVIVNALDNVNAR 709
            ++GQ+K+ VA  A +    ++NI +    V +PE    F+  F++   V++N LDN++AR
Sbjct: 67   HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126

Query: 710  LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 769
             +V++ CL    PL+ESGT G      + I   TE Y     P  K  P+CT+ S P   
Sbjct: 127  RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186

Query: 770  DHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERC 829
             HC+ WA+   + L  K   D N    N     S+   +   ++ D  ER  +       
Sbjct: 187  VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246

Query: 830  ETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHP------LPF 889
               ED   + R  ++  F + +         +AA SN   + +  +R P P      LP 
Sbjct: 247  ---EDIEQYGRKIYDHVFGSNI---------EAALSNEETWKN--RRRPRPIYSKDVLPE 306

Query: 890  SPADQSHLQFVLAAAILRAESYAISIPD-WVKNPRKLGDAVDRVIVPDFMPKKDAKIVTD 949
            S   Q+        ++   +    ++P   +KNP++L       +V  F+          
Sbjct: 307  SLTQQN--GSTQNCSVTDGDLMVSAMPSLGLKNPQELWGLTQNSLV--FI---------- 366

Query: 950  EKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLAN 1009
             +A  L  A      V                    +  + F+KDD     ++ +   AN
Sbjct: 367  -EALKLFFAKRKKVCV-------------------EIGHLTFDKDD--QLAVEFVTAAAN 426

Query: 1010 MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFA 1069
            +RA ++ IP     +AK IAG I+ A+AT+ A+  GL+ +E  KVL     V+ +R T+ 
Sbjct: 427  IRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAIKVLK--KDVDKFRMTYC 481

Query: 1070 ------NLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI-KWLKNK-GLN 1126
                   L L      EP P   +     S T     I      LR+L+ K +K K G+N
Sbjct: 487  LEHPSKKLLLMPIEPYEPNPACYV----CSETPLVLEINTRKSKLRDLVDKIVKTKLGMN 481

BLAST of Carg23870-RA vs. TAIR 10
Match: AT2G21470.1 (SUMO-activating enzyme 2 )

HSP 1 Score: 163.7 bits (413), Expect = 9.0e-40
Identity = 159/545 (29.17%), Postives = 241/545 (44.22%), Query Frame = 0

Query: 590  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 649
            Q  ++ AKV MVG+G +GCE LK LAL G        + I D D IE SNL+RQFLFR  
Sbjct: 7    QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66

Query: 650  NIGQNKSTVAASAAVAINRHLNIEALQNRV-SPETENVFNDSFWENLSVIVNALDNVNAR 709
            ++GQ+K+ VA  A +    ++NI +    V +PE    F+  F++   V++N LDN++AR
Sbjct: 67   HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126

Query: 710  LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 769
             +V++ CL    PL+ESGT G      + I   TE Y     P  K  P+CT+ S P   
Sbjct: 127  RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186

Query: 770  DHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERC 829
             HC+ WA+   + L  K   D N    N     S+   +   ++ D  ER  +       
Sbjct: 187  VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246

Query: 830  ETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQS 889
               ED   + R  ++  F + +         +AA SN   + +  +R P P+        
Sbjct: 247  ---EDIEQYGRKIYDHVFGSNI---------EAALSNEETWKN--RRRPRPI-------- 306

Query: 890  HLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLS 949
            + + VL  ++ +      +               D  ++   MP    K         L 
Sbjct: 307  YSKDVLPESLTQQNGSTQNC-----------SVTDGDLMVSAMPSLGLK-----NPQELW 366

Query: 950  AASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYS 1009
              + +    I  L  KL   +R    G     + F+KDD     ++ +   AN+RA ++ 
Sbjct: 367  GLTQNSLVFIEAL--KLFFAKRKKEIGH----LTFDKDD--QLAVEFVTAAANIRAESFG 426

Query: 1010 IPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFA------N 1069
            IP     +AK IAG I+ A+AT+ A+  GL+ +E  KVL     V+ +R T+        
Sbjct: 427  IPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAIKVLK--KDVDKFRMTYCLEHPSKK 478

Query: 1070 LALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI-KWLKNK-GLNAYSISCG 1126
            L L      EP P   +     S T     I      LR+L+ K +K K G+N   I  G
Sbjct: 487  LLLMPIEPYEPNPACYV----CSETPLVLEINTRKSKLRDLVDKIVKTKLGMNLPLIMHG 478

BLAST of Carg23870-RA vs. TAIR 10
Match: AT2G21470.2 (SUMO-activating enzyme 2 )

HSP 1 Score: 163.7 bits (413), Expect = 9.0e-40
Identity = 159/545 (29.17%), Postives = 241/545 (44.22%), Query Frame = 0

Query: 590  QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 649
            Q  ++ AKV MVG+G +GCE LK LAL G        + I D D IE SNL+RQFLFR  
Sbjct: 7    QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66

Query: 650  NIGQNKSTVAASAAVAINRHLNIEALQNRV-SPETENVFNDSFWENLSVIVNALDNVNAR 709
            ++GQ+K+ VA  A +    ++NI +    V +PE    F+  F++   V++N LDN++AR
Sbjct: 67   HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126

Query: 710  LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 769
             +V++ CL    PL+ESGT G      + I   TE Y     P  K  P+CT+ S P   
Sbjct: 127  RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186

Query: 770  DHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERC 829
             HC+ WA+   + L  K   D N    N     S+   +   ++ D  ER  +       
Sbjct: 187  VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246

Query: 830  ETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQS 889
               ED   + R  ++  F + +         +AA SN   + +  +R P P+        
Sbjct: 247  ---EDIEQYGRKIYDHVFGSNI---------EAALSNEETWKN--RRRPRPI-------- 306

Query: 890  HLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLS 949
            + + VL  ++ +      +               D  ++   MP    K         L 
Sbjct: 307  YSKDVLPESLTQQNGSTQNC-----------SVTDGDLMVSAMPSLGLK-----NPQELW 366

Query: 950  AASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYS 1009
              + +    I  L  KL   +R    G     + F+KDD     ++ +   AN+RA ++ 
Sbjct: 367  GLTQNSLVFIEAL--KLFFAKRKKEIGH----LTFDKDD--QLAVEFVTAAANIRAESFG 426

Query: 1010 IPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFA------N 1069
            IP     +AK IAG I+ A+AT+ A+  GL+ +E  KVL     V+ +R T+        
Sbjct: 427  IPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAIKVLK--KDVDKFRMTYCLEHPSKK 478

Query: 1070 LALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI-KWLKNK-GLNAYSISCG 1126
            L L      EP P   +     S T     I      LR+L+ K +K K G+N   I  G
Sbjct: 487  LLLMPIEPYEPNPACYV----CSETPLVLEINTRKSKLRDLVDKIVKTKLGMNLPLIMHG 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035346.10.0e+00100.00Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. argyros... [more]
KAG6605397.10.0e+0099.47Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022947321.10.0e+00100.00ubiquitin-activating enzyme E1 1-like [Cucurbita moschata] >XP_022947322.1 ubiqu... [more]
XP_023532141.10.0e+0098.99ubiquitin-activating enzyme E1 1-like [Cucurbita pepo subsp. pepo] >XP_023532142... [more]
XP_023006875.10.0e+0098.72ubiquitin-activating enzyme E1 1-like [Cucurbita maxima] >XP_023006876.1 ubiquit... [more]
Match NameE-valueIdentityDescription
P930280.0e+0077.22Ubiquitin-activating enzyme E1 1 OS=Arabidopsis thaliana OX=3702 GN=UBA1 PE=1 SV... [more]
P929740.0e+0076.75Ubiquitin-activating enzyme E1 2 OS=Arabidopsis thaliana OX=3702 GN=UBA2 PE=1 SV... [more]
P209730.0e+0077.25Ubiquitin-activating enzyme E1 1 OS=Triticum aestivum OX=4565 GN=UBA1 PE=1 SV=1[more]
P312510.0e+0077.15Ubiquitin-activating enzyme E1 2 OS=Triticum aestivum OX=4565 GN=UBA2 PE=2 SV=1[more]
P312520.0e+0074.85Ubiquitin-activating enzyme E1 3 OS=Triticum aestivum OX=4565 GN=UBA3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G6A00.0e+00100.00E1 ubiquitin-activating enzyme OS=Cucurbita moschata OX=3662 GN=LOC111451218 PE=... [more]
A0A6J1KZ040.0e+0098.72E1 ubiquitin-activating enzyme OS=Cucurbita maxima OX=3661 GN=LOC111499539 PE=3 ... [more]
A0A0A0KE120.0e+0089.89E1 ubiquitin-activating enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G181590 PE=3 S... [more]
A0A5A7VQ320.0e+0091.76E1 ubiquitin-activating enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CNI40.0e+0092.62E1 ubiquitin-activating enzyme OS=Cucumis melo OX=3656 GN=LOC103502928 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G30110.10.0e+0077.22ubiquitin-activating enzyme 1 [more]
AT5G06460.10.0e+0076.75ubiquitin activating enzyme 2 [more]
AT2G21470.34.1e-4029.17SUMO-activating enzyme 2 [more]
AT2G21470.19.0e-4029.17SUMO-activating enzyme 2 [more]
AT2G21470.29.0e-4029.17SUMO-activating enzyme 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000011Ubiquitin/SUMO-activating enzyme E1PRINTSPR01849UBIQUITINACTcoord: 672..697
score: 51.76
coord: 625..648
score: 74.83
coord: 202..226
score: 76.83
coord: 317..344
score: 60.57
coord: 826..853
score: 47.77
IPR018965Ubiquitin-activating enzyme E1, C-terminalSMARTSM00985UBA_e1_C_a_2coord: 1056..1178
e-value: 5.0E-53
score: 192.1
IPR018965Ubiquitin-activating enzyme E1, C-terminalPFAMPF09358E1_UFDcoord: 1089..1178
e-value: 7.7E-23
score: 81.0
IPR032418Ubiquitin-activating enzyme E1, FCCH domainPFAMPF16190E1_FCCHcoord: 355..426
e-value: 2.6E-27
score: 94.8
IPR042063Ubiquitin-activating enzyme E1, SCCH domainGENE3D1.10.10.2660coord: 750..1025
e-value: 1.5E-193
score: 645.6
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 182..559
e-value: 1.4E-31
score: 361.8
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 578..1077
e-value: 2.3E-74
score: 361.8
IPR042449Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1GENE3D3.50.50.80coord: 171..289
e-value: 2.0E-36
score: 126.7
IPR032420Ubiquitin-activating enzyme E1, four-helix bundlePFAMPF16191E1_4HBcoord: 427..496
e-value: 1.0E-20
score: 73.7
IPR018075Ubiquitin-activating enzyme E1TIGRFAMTIGR01408TIGR01408coord: 175..1182
e-value: 0.0
score: 1680.2
NoneNo IPR availableGENE3D3.40.50.720coord: 598..1076
e-value: 1.5E-193
score: 645.6
NoneNo IPR availableGENE3D3.40.50.12550coord: 300..588
e-value: 2.3E-109
score: 367.1
NoneNo IPR availablePIRSRPIRSR039133-1PIRSR039133-1coord: 580..789
e-value: 9.2E-48
score: 161.1
NoneNo IPR availablePIRSRPIRSR039133-2PIRSR039133-2coord: 580..789
e-value: 9.2E-48
score: 161.1
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 158..1179
NoneNo IPR availablePANTHERPTHR10953:SF215UBIQUITIN-ACTIVATING ENZYME E1 1coord: 158..1179
NoneNo IPR availableCDDcd01491Ube1_repeat1coord: 180..564
e-value: 1.072E-146
score: 440.551
NoneNo IPR availableCDDcd01490Ube1_repeat2coord: 597..1136
e-value: 0.0
score: 717.531
IPR019572Ubiquitin-activating enzyme, SCCH domainPFAMPF10585UBA_e1_thiolCyscoord: 765..1018
e-value: 2.9E-83
score: 279.7
IPR042302Ubiquitin-activating enzyme E1, FCCH domain superfamilyGENE3D2.40.30.180coord: 343..424
e-value: 2.3E-109
score: 367.1
IPR038252Ubiquitin-activating enzyme E1, C-terminal domain superfamilyGENE3D3.10.290.60coord: 1091..1181
e-value: 1.1E-21
score: 79.0
IPR018074Ubiquitin-activating enzyme E1, conserved sitePROSITEPS00536UBIQUITIN_ACTIVAT_1coord: 540..548
IPR033127Ubiquitin-activating enzyme E1, Cys active sitePROSITEPS00865UBIQUITIN_ACTIVAT_2coord: 757..765
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 573..1073
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 175..562

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg23870Carg23870gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg23870-RA:1Carg23870-RA:1exon
Carg23870-RA:2Carg23870-RA:2exon
Carg23870-RA:3Carg23870-RA:3exon
Carg23870-RA:4Carg23870-RA:4exon
Carg23870-RA:5Carg23870-RA:5exon
Carg23870-RA:6Carg23870-RA:6exon
Carg23870-RA:7Carg23870-RA:7exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg23870-RA:cdsCarg23870-RA:cdsCDS
Carg23870-RA:cdsCarg23870-RA:cds_2CDS
Carg23870-RA:cdsCarg23870-RA:cds_3CDS
Carg23870-RA:cdsCarg23870-RA:cds_4CDS
Carg23870-RA:cdsCarg23870-RA:cds_5CDS
Carg23870-RA:cdsCarg23870-RA:cds_6CDS
Carg23870-RA:cdsCarg23870-RA:cds_7CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg23870-RA:three_prime_utrCarg23870-RA:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg23870-RACarg23870-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006464 cellular protein modification process
biological_process GO:0006974 cellular response to DNA damage stimulus
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004839 ubiquitin activating enzyme activity