Homology
BLAST of Carg23870-RA vs. NCBI nr
Match:
KAG7035346.1 (Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2375.9 bits (6156), Expect = 0.0e+00
Identity = 1182/1182 (100.00%), Postives = 1182/1182 (100.00%), Query Frame = 0
Query: 1 VINFTLISILILINFGVPKNCVECKKSILPFFRFLPFHLSALVFTFHFHSSSKKPLHSSS 60
VINFTLISILILINFGVPKNCVECKKSILPFFRFLPFHLSALVFTFHFHSSSKKPLHSSS
Sbjct: 1 VINFTLISILILINFGVPKNCVECKKSILPFFRFLPFHLSALVFTFHFHSSSKKPLHSSS 60
Query: 61 ILSFHSPSPDSFLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQIDNSSSNISNSVQ 120
ILSFHSPSPDSFLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQIDNSSSNISNSVQ
Sbjct: 61 ILSFHSPSPDSFLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQIDNSSSNISNSVQ 120
Query: 121 NAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASGNLNPQDIDEDL 180
NAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASGNLNPQDIDEDL
Sbjct: 121 NAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASGNLNPQDIDEDL 180
Query: 181 HSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSS 240
HSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSS
Sbjct: 181 HSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSS 240
Query: 241 NFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAME 300
NFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAME
Sbjct: 241 NFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAME 300
Query: 301 FNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALV 360
FNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALV
Sbjct: 301 FNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALV 360
Query: 361 SCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGI 420
SCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGI
Sbjct: 361 SCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGI 420
Query: 421 VTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAG 480
VTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAG
Sbjct: 421 VTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAG 480
Query: 481 SEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLK 540
SEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLK
Sbjct: 481 SEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLK 540
Query: 541 ACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFM 600
ACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFM
Sbjct: 541 ACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFM 600
Query: 601 VGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAA 660
VGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAA
Sbjct: 601 VGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAA 660
Query: 661 SAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQK 720
SAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQK
Sbjct: 661 SAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQK 720
Query: 721 PLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFE 780
PLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFE
Sbjct: 721 PLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFE 780
Query: 781 GLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFEDCITWARL 840
GLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFEDCITWARL
Sbjct: 781 GLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFEDCITWARL 840
Query: 841 KFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILR 900
KFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILR
Sbjct: 841 KFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILR 900
Query: 901 AESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHD 960
AESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHD
Sbjct: 901 AESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHD 960
Query: 961 LVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFI 1020
LVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFI
Sbjct: 961 LVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFI 1020
Query: 1021 AGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIK 1080
AGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIK
Sbjct: 1021 AGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIK 1080
Query: 1081 HRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKV 1140
HRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKV
Sbjct: 1081 HRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKV 1140
Query: 1141 VDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
VDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF
Sbjct: 1141 VDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1182
BLAST of Carg23870-RA vs. NCBI nr
Match:
KAG6605397.1 (Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2265.7 bits (5870), Expect = 0.0e+00
Identity = 1127/1133 (99.47%), Postives = 1130/1133 (99.74%), Query Frame = 0
Query: 50 SSSKKPLHSSSILSFHSPSPDSFLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQID 109
+S KPLHSSSILSFHSPSPDSFLPFFLSLCYAPNR LLHFMLPRKRAGEEGAVLEQQID
Sbjct: 1134 TSHNKPLHSSSILSFHSPSPDSFLPFFLSLCYAPNR-LLHFMLPRKRAGEEGAVLEQQID 1193
Query: 110 NSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASG 169
NSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASG
Sbjct: 1194 NSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMASG 1253
Query: 170 NLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHD 229
NLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHD
Sbjct: 1254 NLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHD 1313
Query: 230 EGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVV 289
EGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLV+EQLSKFEVVV
Sbjct: 1314 EGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVKEQLSKFEVVV 1373
Query: 290 FTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 349
FTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII
Sbjct: 1374 FTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGII 1433
Query: 350 ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDT 409
ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDT
Sbjct: 1434 ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDT 1493
Query: 410 TNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKF 469
TNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKF
Sbjct: 1494 TNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKF 1553
Query: 470 VTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 529
VTELGRFPVAGSEDDAQKLISVASNMN+SLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM
Sbjct: 1554 VTELGRFPVAGSEDDAQKLISVASNMNESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAM 1613
Query: 530 FGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKL 589
FGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKL
Sbjct: 1614 FGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKL 1673
Query: 590 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 649
QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW
Sbjct: 1674 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 1733
Query: 650 NIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARL 709
NIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARL
Sbjct: 1734 NIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARL 1793
Query: 710 YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 769
YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID
Sbjct: 1794 YVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID 1853
Query: 770 HCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCE 829
HCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCE
Sbjct: 1854 HCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCE 1913
Query: 830 TFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSH 889
TFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSH
Sbjct: 1914 TFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSH 1973
Query: 890 LQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSA 949
LQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSA
Sbjct: 1974 LQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSA 2033
Query: 950 ASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSI 1009
ASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSI
Sbjct: 2034 ASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSI 2093
Query: 1010 PEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFS 1069
PEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFS
Sbjct: 2094 PEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFS 2153
Query: 1070 MAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMF 1129
MAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMF
Sbjct: 2154 MAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMF 2213
Query: 1130 PRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
PRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF
Sbjct: 2214 PRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 2265
BLAST of Carg23870-RA vs. NCBI nr
Match:
XP_022947321.1 (ubiquitin-activating enzyme E1 1-like [Cucurbita moschata] >XP_022947322.1 ubiquitin-activating enzyme E1 1-like [Cucurbita moschata] >XP_022947323.1 ubiquitin-activating enzyme E1 1-like [Cucurbita moschata])
HSP 1 Score: 2200.2 bits (5700), Expect = 0.0e+00
Identity = 1092/1092 (100.00%), Postives = 1092/1092 (100.00%), Query Frame = 0
Query: 91 MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 150
MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA
Sbjct: 1 MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 60
Query: 151 VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 210
VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG
Sbjct: 61 VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 120
Query: 211 AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 270
AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV
Sbjct: 121 AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 180
Query: 271 HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 330
HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF
Sbjct: 181 HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 240
Query: 331 GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 390
GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG
Sbjct: 241 GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 300
Query: 391 KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 450
KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF
Sbjct: 301 KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 360
Query: 451 SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK 510
SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK
Sbjct: 361 SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK 420
Query: 511 LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 570
LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE
Sbjct: 421 LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 480
Query: 571 FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 630
FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT
Sbjct: 481 FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 540
Query: 631 DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 690
DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS
Sbjct: 541 DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 600
Query: 691 FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 750
FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD
Sbjct: 601 FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 660
Query: 751 PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 810
PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA
Sbjct: 661 PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 720
Query: 811 QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 870
QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW
Sbjct: 721 QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 780
Query: 871 SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF 930
SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF
Sbjct: 781 SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF 840
Query: 931 MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 990
MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN
Sbjct: 841 MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 900
Query: 991 YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 1050
YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG
Sbjct: 901 YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 960
Query: 1051 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1110
HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK
Sbjct: 961 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1020
Query: 1111 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1170
GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED
Sbjct: 1021 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1080
Query: 1171 NDIDIPLVSVYF 1183
NDIDIPLVSVYF
Sbjct: 1081 NDIDIPLVSVYF 1092
BLAST of Carg23870-RA vs. NCBI nr
Match:
XP_023532141.1 (ubiquitin-activating enzyme E1 1-like [Cucurbita pepo subsp. pepo] >XP_023532142.1 ubiquitin-activating enzyme E1 1-like [Cucurbita pepo subsp. pepo] >XP_023532143.1 ubiquitin-activating enzyme E1 1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2172.9 bits (5629), Expect = 0.0e+00
Identity = 1081/1092 (98.99%), Postives = 1085/1092 (99.36%), Query Frame = 0
Query: 91 MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 150
MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA
Sbjct: 1 MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 60
Query: 151 VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 210
VPT NNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG
Sbjct: 61 VPTANNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 120
Query: 211 AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 270
AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV
Sbjct: 121 AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 180
Query: 271 HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 330
HTL TKLV+EQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF
Sbjct: 181 HTLATKLVKEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 240
Query: 331 GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 390
GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG
Sbjct: 241 GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 300
Query: 391 KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 450
KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF
Sbjct: 301 KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 360
Query: 451 SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK 510
SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMN+SLGDGRVEDIN K
Sbjct: 361 SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNESLGDGRVEDINLK 420
Query: 511 LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 570
LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE
Sbjct: 421 LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 480
Query: 571 FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 630
FRPLNSRYDAQISVFGSKLQKKLENA+VFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT
Sbjct: 481 FRPLNSRYDAQISVFGSKLQKKLENAQVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 540
Query: 631 DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 690
DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS
Sbjct: 541 DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 600
Query: 691 FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 750
FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD
Sbjct: 601 FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 660
Query: 751 PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 810
PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA
Sbjct: 661 PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 720
Query: 811 QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 870
QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW
Sbjct: 721 QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 780
Query: 871 SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF 930
SAPKRFPHPLPFS ADQSHLQFVLAAAILRAESYAISIPDWVKN KL DAVDRVIVPDF
Sbjct: 781 SAPKRFPHPLPFSTADQSHLQFVLAAAILRAESYAISIPDWVKNHGKLADAVDRVIVPDF 840
Query: 931 MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 990
MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN
Sbjct: 841 MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 900
Query: 991 YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 1050
YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG
Sbjct: 901 YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 960
Query: 1051 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1110
HKVE+YRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK
Sbjct: 961 HKVENYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1020
Query: 1111 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1170
GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED
Sbjct: 1021 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1080
Query: 1171 NDIDIPLVSVYF 1183
NDIDIPLVSVYF
Sbjct: 1081 NDIDIPLVSVYF 1092
BLAST of Carg23870-RA vs. NCBI nr
Match:
XP_023006875.1 (ubiquitin-activating enzyme E1 1-like [Cucurbita maxima] >XP_023006876.1 ubiquitin-activating enzyme E1 1-like [Cucurbita maxima] >XP_023006877.1 ubiquitin-activating enzyme E1 1-like [Cucurbita maxima])
HSP 1 Score: 2172.1 bits (5627), Expect = 0.0e+00
Identity = 1081/1095 (98.72%), Postives = 1087/1095 (99.27%), Query Frame = 0
Query: 91 MLPRKRAGEEGAVLEQQIDNSS---SNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNV 150
MLPRKRAGEEGAVLEQQIDNSS SNISNSVQNAGVSLIKKHRIDNCNVDSNVN NTNV
Sbjct: 1 MLPRKRAGEEGAVLEQQIDNSSSNISNISNSVQNAGVSLIKKHRIDNCNVDSNVNFNTNV 60
Query: 151 AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 210
AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ
Sbjct: 61 AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 120
Query: 211 GLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS 270
GLGAEIAKNVILAGVKSVTLHD+GVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS
Sbjct: 121 GLGAEIAKNVILAGVKSVTLHDDGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS 180
Query: 271 VIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF 330
VIVHTL TKLV+EQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF
Sbjct: 181 VIVHTLTTKLVKEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF 240
Query: 331 CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL 390
CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL
Sbjct: 241 CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL 300
Query: 391 NDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL 450
NDGKPRRIKNCRAYSFTLDEDTTN+GIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL
Sbjct: 301 NDGKPRRIKNCRAYSFTLDEDTTNYGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL 360
Query: 451 SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDI 510
SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMN+SLGDGRVEDI
Sbjct: 361 SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNESLGDGRVEDI 420
Query: 511 NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD 570
NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD
Sbjct: 421 NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD 480
Query: 571 ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL 630
ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL
Sbjct: 481 ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL 540
Query: 631 TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF 690
TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF
Sbjct: 541 TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF 600
Query: 691 NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA 750
NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA
Sbjct: 601 NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA 660
Query: 751 SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA 810
SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA
Sbjct: 661 SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA 720
Query: 811 GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA 870
GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA
Sbjct: 721 GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA 780
Query: 871 PFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIV 930
PFWSAPKRFPHPLPFS ADQSHLQFVLAAAILRAESYAISIPDWVKNPRKL DAVDRVIV
Sbjct: 781 PFWSAPKRFPHPLPFSTADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLADAVDRVIV 840
Query: 931 PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDD 990
PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLV+KLEDTRRNLPEGFRMKPIQFEKDD
Sbjct: 841 PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVDKLEDTRRNLPEGFRMKPIQFEKDD 900
Query: 991 DTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL 1050
DTNYHMDLIAGLANMRARN+SIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL
Sbjct: 901 DTNYHMDLIAGLANMRARNFSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL 960
Query: 1051 DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL 1110
DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL
Sbjct: 961 DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL 1020
Query: 1111 KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACED 1170
KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPY RHLDVVVACED
Sbjct: 1021 KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYCRHLDVVVACED 1080
Query: 1171 DEDNDIDIPLVSVYF 1183
DEDNDIDIPLVSVYF
Sbjct: 1081 DEDNDIDIPLVSVYF 1095
BLAST of Carg23870-RA vs. ExPASy Swiss-Prot
Match:
P93028 (Ubiquitin-activating enzyme E1 1 OS=Arabidopsis thaliana OX=3702 GN=UBA1 PE=1 SV=1)
HSP 1 Score: 1726.5 bits (4470), Expect = 0.0e+00
Identity = 834/1080 (77.22%), Postives = 956/1080 (88.52%), Query Frame = 0
Query: 103 VLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGA 162
+L ++ ++ N++ + ++ KK RID S+ S++ +A+ G + +
Sbjct: 1 MLHKRASEANDKNDNTIIGSDLASSKKRRIDFTESSSD-KSSSILASGSSRGFHGDSVVQ 60
Query: 163 SLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGV 222
+ MA GN N Q+IDEDLHSRQLAVYGRETMR+LFASNVLISGM GLGAEIAKN+ILAGV
Sbjct: 61 QIDMAFGNSNRQEIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEIAKNLILAGV 120
Query: 223 KSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQL 282
KSVTLHDE VVELWDLSSNFVFSE D+GKNRA AS QKLQDLNN+V+V +L L +E L
Sbjct: 121 KSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSSLTKSLNKEDL 180
Query: 283 SKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGE 342
S F+VVVF+D +++A+EF+D+CH+HQPPI+F+K++VRGLFGSVFCDFGPEF V DV GE
Sbjct: 181 SGFQVVVFSDISMERAIEFDDYCHSHQPPIAFVKADVRGLFGSVFCDFGPEFAVLDVDGE 240
Query: 343 DPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYS 402
+PHTGIIASISN+N A +SCVDDERLEF+DGDLVVFSEV GMTELNDGKPR+IK+ R YS
Sbjct: 241 EPHTGIIASISNENQAFISCVDDERLEFEDGDLVVFSEVEGMTELNDGKPRKIKSTRPYS 300
Query: 403 FTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLA 462
FTLDEDTTN+G Y KGGIVTQVKQPK+LNFKPLREA+ DPGDFL SDFSKFDRPPL+HLA
Sbjct: 301 FTLDEDTTNYGTYVKGGIVTQVKQPKLLNFKPLREALKDPGDFLFSDFSKFDRPPLLHLA 360
Query: 463 FLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAV 522
F ALD F E GRFPVAGSE+DAQKLIS+A+ +N GD +VE+++ KLLRHF+FGAKAV
Sbjct: 361 FQALDHFKAEAGRFPVAGSEEDAQKLISIATAINTGQGDLKVENVDQKLLRHFSFGAKAV 420
Query: 523 LNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQI 582
LNPMAAMFGGIV QEV+KACSGKFHPL QFFYFDSVESLP+E +D+S+F P NSRYDAQI
Sbjct: 421 LNPMAAMFGGIVGQEVVKACSGKFHPLFQFFYFDSVESLPSEPVDSSDFAPRNSRYDAQI 480
Query: 583 SVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSR 642
SVFG+K QKKLE+AKVF VGSGALGCEFLKNLALMGVSC ++GKLT+TDDD+IEKSNLSR
Sbjct: 481 SVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGVSCGSQGKLTVTDDDIIEKSNLSR 540
Query: 643 QFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNAL 702
QFLFRDWNIGQ KSTVAASAA IN NIEALQNRV ETENVF+D+FWENL+V+VNAL
Sbjct: 541 QFLFRDWNIGQAKSTVAASAAAVINPRFNIEALQNRVGAETENVFDDAFWENLTVVVNAL 600
Query: 703 DNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVH 762
DNVNARLYVD RCLYFQKPLLESGTLG KCNTQ VIPHLTENYGASRDPPEKQAPMCTVH
Sbjct: 601 DNVNARLYVDSRCLYFQKPLLESGTLGTKCNTQSVIPHLTENYGASRDPPEKQAPMCTVH 660
Query: 763 SFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLEC 822
SFPHNIDHCLTWARSEFEGLLEKTP++VNAYLS+P EY +SMM+AGDAQ+RDTLER++EC
Sbjct: 661 SFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSSPVEYTNSMMSAGDAQARDTLERIVEC 720
Query: 823 LDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPF 882
L++E+CETF+DC+TWARL+FEDYF NRVKQLIYTFPEDAATS GAPFWSAPKRFP PL +
Sbjct: 721 LEKEKCETFQDCLTWARLRFEDYFVNRVKQLIYTFPEDAATSTGAPFWSAPKRFPRPLQY 780
Query: 883 SPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDE 942
S +D S L F+ A AILRAE++ I IP+W KNP++ +AVDRVIVPDF P++DAKIVTDE
Sbjct: 781 SSSDPSLLNFITATAILRAETFGIPIPEWTKNPKEAAEAVDRVIVPDFEPRQDAKIVTDE 840
Query: 943 KATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANM 1002
KAT+L+ ASVDDAAVI DL+ K++ R NL FRMKPIQFEKDDDTNYHMD+IAGLANM
Sbjct: 841 KATTLTTASVDDAAVIDDLIAKIDQCRHNLSPDFRMKPIQFEKDDDTNYHMDVIAGLANM 900
Query: 1003 RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFAN 1062
RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVE YRNTFAN
Sbjct: 901 RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEAYRNTFAN 960
Query: 1063 LALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSC 1122
LALPLFSMAEP+PPKV+KHRDM+WTVWDRW++K NPTLRE+++WL++KGL+AYSISCGSC
Sbjct: 961 LALPLFSMAEPLPPKVVKHRDMAWTVWDRWVLKGNPTLREVLQWLEDKGLSAYSISCGSC 1020
Query: 1123 LLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1182
LL+NSMF RHK+RMDKKVVDLARD+AKVELPPYR HLDVVVACED++DND+DIPLVS+YF
Sbjct: 1021 LLFNSMFTRHKERMDKKVVDLARDVAKVELPPYRNHLDVVVACEDEDDNDVDIPLVSIYF 1079
BLAST of Carg23870-RA vs. ExPASy Swiss-Prot
Match:
P92974 (Ubiquitin-activating enzyme E1 2 OS=Arabidopsis thaliana OX=3702 GN=UBA2 PE=1 SV=1)
HSP 1 Score: 1694.1 bits (4386), Expect = 0.0e+00
Identity = 822/1071 (76.75%), Postives = 933/1071 (87.11%), Query Frame = 0
Query: 115 ISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMA---SGNL 174
+ ++ + S +KK RID+ N + ++++ N+I + + MA + N
Sbjct: 6 VKENIIASASSPMKKRRIDHTESADGSAINASNSSSIGLNNSIGGNDTVMSMAEFGNDNS 65
Query: 175 NPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEG 234
N Q+IDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG EIAKN+ILAGVKSVTLHDE
Sbjct: 66 NNQEIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGVEIAKNIILAGVKSVTLHDEN 125
Query: 235 VVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFT 294
VVELWDLSSNFVF+E DIGKNRALAS KLQ+LNN+V V TL KL +EQLS F+VVVF
Sbjct: 126 VVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLTGKLTKEQLSDFQVVVFV 185
Query: 295 DTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIAS 354
D +KA E +D+CH+HQPPI+FIK++VRGLFGS+FCDFGP FTV DV GE+PH+GIIAS
Sbjct: 186 DISFEKATEIDDYCHSHQPPIAFIKADVRGLFGSLFCDFGPHFTVLDVDGEEPHSGIIAS 245
Query: 355 ISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTN 414
+SN+NP VSCVDDERLEF+DG+LVVFSEV GMTELNDGKPR+IKN + +SFTL+EDT++
Sbjct: 246 VSNENPGFVSCVDDERLEFEDGNLVVFSEVEGMTELNDGKPRKIKNVKPFSFTLEEDTSS 305
Query: 415 FGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVT 474
+G Y KGGIVTQVKQPKVLNFKPLREA+ DPGDFLLSDFSKFDRPPL+HLAF ALD+F +
Sbjct: 306 YGQYMKGGIVTQVKQPKVLNFKPLREALKDPGDFLLSDFSKFDRPPLLHLAFQALDRFSS 365
Query: 475 ELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFG 534
+ GRFP AGSE+DAQKL+ +A ++N+ LGD R+ED+N KLLRH AFG++AVLNPMAAMFG
Sbjct: 366 QAGRFPFAGSEEDAQKLVEIAVDINEGLGDARLEDVNSKLLRHLAFGSRAVLNPMAAMFG 425
Query: 535 GIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQK 594
GIV QEV+KACSGKFHP+ QFFYFDSVESLP E LDASEFRP NSRYDAQISVFGS LQK
Sbjct: 426 GIVGQEVVKACSGKFHPIFQFFYFDSVESLPKEPLDASEFRPQNSRYDAQISVFGSTLQK 485
Query: 595 KLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNI 654
KLE+A+VF+VG+GALGCEFLKNLALMGVSC +GKLT+TDDDVIEKSNLSRQFLFRDWNI
Sbjct: 486 KLEDARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQFLFRDWNI 545
Query: 655 GQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYV 714
GQ KSTVAA+AA IN LNI+ALQNRV PETENVF+DSFWENL+V+VNALDNV ARLYV
Sbjct: 546 GQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENLTVVVNALDNVTARLYV 605
Query: 715 DQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC 774
D RC+YFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC
Sbjct: 606 DSRCVYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC 665
Query: 775 LTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETF 834
LTWARSEFEGLLEKTP++VNAYLS+P EY +M AGDAQ+RDTL RV+ECL++E+C +F
Sbjct: 666 LTWARSEFEGLLEKTPAEVNAYLSDPVEYMKAMRTAGDAQARDTLGRVVECLEKEKCNSF 725
Query: 835 EDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQ 894
+DCITWARL+FEDYFANRVKQL YTFPEDAATS GAPFWSAPKRFP PL FS D SH+
Sbjct: 726 QDCITWARLRFEDYFANRVKQLCYTFPEDAATSTGAPFWSAPKRFPRPLQFSSTDLSHIN 785
Query: 895 FVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAAS 954
FV+AA+ILRAE++ I P+W K L +AV+RVIVPDF PKKDA IVTDEKAT+LS AS
Sbjct: 786 FVMAASILRAETFGIPTPEWAKTRAGLAEAVERVIVPDFEPKKDATIVTDEKATTLSTAS 845
Query: 955 VDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPE 1014
VDDAAVI +L KL R +L FRMK IQFEKDDDTNYHMD+IAGLANMRARNYS+PE
Sbjct: 846 VDDAAVIDELNAKLVRCRMSLQPEFRMKAIQFEKDDDTNYHMDMIAGLANMRARNYSVPE 905
Query: 1015 VDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMA 1074
VDKLKAKFIAGRIIPAIATSTAMATG VCLE+YKVLDG HKVEDYRNTFANLALPLFSMA
Sbjct: 906 VDKLKAKFIAGRIIPAIATSTAMATGFVCLEMYKVLDGSHKVEDYRNTFANLALPLFSMA 965
Query: 1075 EPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPR 1134
EPVPPKV+KH+D SWTVWDRW+++ NPTLREL+ WLK KGLNAYSISCGS LLYNSMF R
Sbjct: 966 EPVPPKVVKHQDQSWTVWDRWVMRGNPTLRELLDWLKEKGLNAYSISCGSSLLYNSMFSR 1025
Query: 1135 HKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
HK+RM+++VVDLARD+A VELP YRRH+DVVVACEDD D D+DIPLVSVYF
Sbjct: 1026 HKERMNRRVVDLARDVAGVELPAYRRHVDVVVACEDDNDADVDIPLVSVYF 1076
BLAST of Carg23870-RA vs. ExPASy Swiss-Prot
Match:
P20973 (Ubiquitin-activating enzyme E1 1 OS=Triticum aestivum OX=4565 GN=UBA1 PE=1 SV=1)
HSP 1 Score: 1661.7 bits (4302), Expect = 0.0e+00
Identity = 791/1024 (77.25%), Postives = 909/1024 (88.77%), Query Frame = 0
Query: 160 DGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVIL 219
+G + + GN +IDEDLHSRQLAVYGRETM++LF SNVL+SG+QGLGAEIAKN++L
Sbjct: 31 EGDAAMAGRGN----EIDEDLHSRQLAVYGRETMKRLFGSNVLVSGLQGLGAEIAKNLVL 90
Query: 220 AGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVE 279
AGVKSVTLHD+G VELWDLSSNF SE+D+G+NRA A QKLQ+LNN+V+V L L +
Sbjct: 91 AGVKSVTLHDDGNVELWDLSSNFFLSENDVGQNRAQACVQKLQELNNAVLVSALTGDLTK 150
Query: 280 EQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDV 339
E LSKF+ VVFTD LDKA+EF+D+CH+ QPPI+FIKSEVRGLFGSVFCDFGPEFTV DV
Sbjct: 151 EHLSKFQAVVFTDISLDKAIEFDDYCHSQQPPIAFIKSEVRGLFGSVFCDFGPEFTVLDV 210
Query: 340 YGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCR 399
GE+PHTGI+ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPR++KN R
Sbjct: 211 DGEEPHTGIVASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRKVKNAR 270
Query: 400 AYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLI 459
YSF L+EDT++FG Y +GGIVTQVK PKV+ FKPL+EA+++PG+FL+SDFSKF+RPPL+
Sbjct: 271 PYSFFLEEDTSSFGAYVRGGIVTQVKPPKVIKFKPLKEAMSEPGEFLMSDFSKFERPPLL 330
Query: 460 HLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGA 519
HLAF ALDKF TEL RFPVAGS DD Q++I A ++N +LGD ++E+I+ KLL HFA G+
Sbjct: 331 HLAFQALDKFRTELSRFPVAGSTDDVQRVIEYAISINDTLGDRKLEEIDKKLLHHFASGS 390
Query: 520 KAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYD 579
+AVLNPMAAMFGGIV QEV+KACSGKFHPL QFFYFDSVESLP + L+ + +P NSRYD
Sbjct: 391 RAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFDSVESLPVDPLEPGDLKPKNSRYD 450
Query: 580 AQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSN 639
AQISVFGSKLQ KLE AK+FMVGSGALGCEFLKNLALMG+SCS G LT+TDDDVIEKSN
Sbjct: 451 AQISVFGSKLQNKLEEAKIFMVGSGALGCEFLKNLALMGISCSQNGNLTLTDDDVIEKSN 510
Query: 640 LSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIV 699
LSRQFLFRDWNIGQ KSTVAA+AA+ IN L++EALQNR SPETENVFND+FWENL +V
Sbjct: 511 LSRQFLFRDWNIGQPKSTVAATAAMVINPKLHVEALQNRASPETENVFNDAFWENLDAVV 570
Query: 700 NALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC 759
NALDNV AR+Y+D RC+YFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC
Sbjct: 571 NALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC 630
Query: 760 TVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERV 819
TVHSFPHNIDHCLTWARSEFEGLLEKTP++VNA+LSNP+ Y S+ AGDAQ+RD LERV
Sbjct: 631 TVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAFLSNPTTYISAARTAGDAQARDQLERV 690
Query: 820 LECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHP 879
+ECLDR++CETF+D ITWARLKFEDYF+NRVKQL +TFPED+ TS+GAPFWSAPKRFP P
Sbjct: 691 IECLDRDKCETFQDSITWARLKFEDYFSNRVKQLTFTFPEDSMTSSGAPFWSAPKRFPRP 750
Query: 880 LPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKL-GDAVDRVIVPDFMPKKDAKI 939
+ FS +DQS L F+LAAAILRAE++ I IP+W K P KL +AVD+VIVPDF PK+ KI
Sbjct: 751 VEFSSSDQSQLSFILAAAILRAETFGIPIPEWAKTPNKLAAEAVDKVIVPDFQPKQGVKI 810
Query: 940 VTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAG 999
VT EKATSLS+ASVDDAAVI +L+ KLE+ + LP GF M PIQFEKDDDTN+HMD+IAG
Sbjct: 811 VTHEKATSLSSASVDDAAVIEELIAKLEEVSKTLPSGFHMNPIQFEKDDDTNFHMDVIAG 870
Query: 1000 LANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRN 1059
ANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK L GGHKVEDYRN
Sbjct: 871 FANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKALAGGHKVEDYRN 930
Query: 1060 TFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSIS 1119
TFANLA+PLFS+AEPVPPK IKH+++SWTVWDRW + N TLREL++WLK KGLNAYSIS
Sbjct: 931 TFANLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELLEWLKEKGLNAYSIS 990
Query: 1120 CGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLV 1179
CG+ LLYNSMFPRHK+R+D+KVVD+AR++AK+E+P YRRHLDVVVACEDD+DND+DIPLV
Sbjct: 991 CGTSLLYNSMFPRHKERLDRKVVDVAREVAKMEVPSYRRHLDVVVACEDDDDNDVDIPLV 1050
Query: 1180 SVYF 1183
SVYF
Sbjct: 1051 SVYF 1050
BLAST of Carg23870-RA vs. ExPASy Swiss-Prot
Match:
P31251 (Ubiquitin-activating enzyme E1 2 OS=Triticum aestivum OX=4565 GN=UBA2 PE=2 SV=1)
HSP 1 Score: 1659.0 bits (4295), Expect = 0.0e+00
Identity = 790/1024 (77.15%), Postives = 907/1024 (88.57%), Query Frame = 0
Query: 160 DGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVIL 219
+G + + GN +IDEDLHSRQLAVYGRETM+ LF SNVL+SG+QGLGAEIAKN++L
Sbjct: 31 EGDAAMAGRGN----EIDEDLHSRQLAVYGRETMKPLFGSNVLVSGLQGLGAEIAKNLVL 90
Query: 220 AGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVE 279
AGVKSVTLHD+G VELWDLSSNF SE+D+G+NRA A QKLQ+LNN+V+V L L +
Sbjct: 91 AGVKSVTLHDDGNVELWDLSSNFFLSENDVGQNRAQACVQKLQELNNAVLVSALTGDLTK 150
Query: 280 EQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDV 339
E LSKF+ VVFTD LDKA+EF+D+CH+HQPPI+FIKSEVRGLFGSVFCDFGPEFTV DV
Sbjct: 151 EHLSKFQAVVFTDISLDKAIEFDDYCHSHQPPIAFIKSEVRGLFGSVFCDFGPEFTVLDV 210
Query: 340 YGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCR 399
GE+PHTGI+ASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPR++KN R
Sbjct: 211 DGEEPHTGIVASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRKVKNAR 270
Query: 400 AYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLI 459
YSF L+EDT++FG Y +GGIVTQVK PKV+ FKPL+EA+++PG+FL+SDFSKF+RPPL+
Sbjct: 271 PYSFFLEEDTSSFGAYVRGGIVTQVKPPKVIKFKPLKEAMSEPGEFLMSDFSKFERPPLL 330
Query: 460 HLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGA 519
HLAF ALDKF TEL RFPVAGS DD Q++I A ++N +LGD ++E+I+ KLL HFA G+
Sbjct: 331 HLAFQALDKFRTELSRFPVAGSTDDVQRVIEYAISINDTLGDRKLEEIDKKLLHHFASGS 390
Query: 520 KAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYD 579
+AVLNPMAAMFGGIV QEV+KACSGKFHPL QFFYFDSVESLP + L+ + +P NSRYD
Sbjct: 391 RAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFDSVESLPVDPLEPGDLKPKNSRYD 450
Query: 580 AQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSN 639
AQISVFGS LQ KLE AK+FMVGSGALGCEFLKNLALMG+SCS G LT+TDDDVIEKSN
Sbjct: 451 AQISVFGSTLQNKLEEAKIFMVGSGALGCEFLKNLALMGISCSQNGNLTVTDDDVIEKSN 510
Query: 640 LSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIV 699
LSRQFLFRDWNIGQ KSTVAA+AA+ IN L++EALQNR SPETENVFND+FWENL +V
Sbjct: 511 LSRQFLFRDWNIGQPKSTVAATAAMVINPKLHVEALQNRASPETENVFNDAFWENLDAVV 570
Query: 700 NALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC 759
NALDNV AR+Y+D RC+YFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC
Sbjct: 571 NALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMC 630
Query: 760 TVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERV 819
TVHSFPHNIDHCLTWARSEFEGLLEKTP++VNA+LSNP+ Y S+ AGDAQ+RD LERV
Sbjct: 631 TVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAFLSNPTTYISAARTAGDAQARDQLERV 690
Query: 820 LECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHP 879
+ECLDR++CETF+D ITWARLKFEDYF+NRVKQL +TFPED+ TS+GAPFWSAPKRFP P
Sbjct: 691 IECLDRDKCETFQDSITWARLKFEDYFSNRVKQLTFTFPEDSMTSSGAPFWSAPKRFPRP 750
Query: 880 LPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKL-GDAVDRVIVPDFMPKKDAKI 939
+ FS +D S L F+LAAAILRAE++ I I +W K P KL +AVD+VIVPDF PK+ KI
Sbjct: 751 VEFSSSDPSQLSFILAAAILRAETFGIPISEWAKTPNKLAAEAVDKVIVPDFQPKQGVKI 810
Query: 940 VTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAG 999
VTDEKATSLS+ASVDDAAVI +L+ KLE+ + LP GF M PIQFEKDDDTN+HMD+IAG
Sbjct: 811 VTDEKATSLSSASVDDAAVIEELIAKLEEVSKTLPSGFHMNPIQFEKDDDTNFHMDVIAG 870
Query: 1000 LANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRN 1059
ANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK L GGHKVEDYRN
Sbjct: 871 FANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKALAGGHKVEDYRN 930
Query: 1060 TFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSIS 1119
TFANLA+PLFS+AEPVPPK IKH+++SWTVWDRW + N TLREL++WLK KGLNAYSIS
Sbjct: 931 TFANLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELLEWLKEKGLNAYSIS 990
Query: 1120 CGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLV 1179
CG+ LLYNSMFPRHK+R+D+KVVD+AR++AK+E+P YRRHLDVVVACEDD+DND+DIPLV
Sbjct: 991 CGTSLLYNSMFPRHKERLDRKVVDVAREVAKMEVPSYRRHLDVVVACEDDDDNDVDIPLV 1050
Query: 1180 SVYF 1183
SVYF
Sbjct: 1051 SVYF 1050
BLAST of Carg23870-RA vs. ExPASy Swiss-Prot
Match:
P31252 (Ubiquitin-activating enzyme E1 3 OS=Triticum aestivum OX=4565 GN=UBA3 PE=1 SV=1)
HSP 1 Score: 1597.0 bits (4134), Expect = 0.0e+00
Identity = 756/1010 (74.85%), Postives = 886/1010 (87.72%), Query Frame = 0
Query: 173 PQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGV 232
PQ+IDEDLHSRQLAVYGRETMR+LFAS+VL+SG+ GLGAEIAKN+ LAGVKSVT+HD
Sbjct: 43 PQEIDEDLHSRQLAVYGRETMRRLFASDVLVSGLNGLGAEIAKNLALAGVKSVTIHDVKT 102
Query: 233 VELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTD 292
V++WDLS NF SE DIGKNRA A KLQ+LNN+V++ L +L E LSKF+ VVFTD
Sbjct: 103 VKMWDLSGNFFLSEDDIGKNRAAACVAKLQELNNAVLISALTEELTTEHLSKFQAVVFTD 162
Query: 293 TGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASI 352
LDKA EF+D+CHNHQPPISFIKSEV GLFGSVFCDFGP+FTV DV GEDPHTGIIASI
Sbjct: 163 IDLDKAYEFDDYCHNHQPPISFIKSEVCGLFGSVFCDFGPKFTVLDVDGEDPHTGIIASI 222
Query: 353 SNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNF 412
SNDNPAL+SCVDDERLEFQDGDLVVFSEVHGMTELNDGKPR++KN R +SF+++EDT+NF
Sbjct: 223 SNDNPALISCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRKVKNARPFSFSIEEDTSNF 282
Query: 413 GIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTE 472
GIY KGGIVTQVK+PKVL FK LR+A+ DPG+ LLSDFSKF+RPP++HLAF ALDKF +
Sbjct: 283 GIYVKGGIVTQVKEPKVLCFKALRDAMTDPGEVLLSDFSKFERPPVLHLAFQALDKFKKD 342
Query: 473 LGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGG 532
GR P AG E+DA + +A+ +N++ D +++ I+ KL R FA G++AVLNPMAAMFGG
Sbjct: 343 HGRCPAAGCEEDAHSFLKIAAAINEASADRKLDTIDEKLFRQFASGSRAVLNPMAAMFGG 402
Query: 533 IVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKK 592
IV QEV+KACSGKFHPL QFFYFDSVESLPT L+ + +P N+RYDAQ+SVFGSKLQKK
Sbjct: 403 IVGQEVVKACSGKFHPLNQFFYFDSVESLPTYPLEPQDLKPSNNRYDAQVSVFGSKLQKK 462
Query: 593 LENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIG 652
+E A F+VGSGALGCEFLKNLALMGVSCS++GKLTITDDD+IEKSNLSRQFLFRDWNIG
Sbjct: 463 MEEANTFVVGSGALGCEFLKNLALMGVSCSSKGKLTITDDDIIEKSNLSRQFLFRDWNIG 522
Query: 653 QNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVD 712
Q KSTVAA+AA AIN L+I+ALQNR P+TENVF+D+FWE L V++NALDNVNAR+Y+D
Sbjct: 523 QAKSTVAATAASAINPSLHIDALQNRACPDTENVFHDTFWEGLDVVINALDNVNARMYMD 582
Query: 713 QRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 772
RCLYFQKPLLESGTLGAKCN QMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL
Sbjct: 583 MRCLYFQKPLLESGTLGAKCNIQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCL 642
Query: 773 TWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFE 832
TWARSEFEGLLEKTP++VN++LSNP++YA++M AGDAQ+R+ LERV ECL+++RC TF+
Sbjct: 643 TWARSEFEGLLEKTPNEVNSFLSNPAQYAAAMRKAGDAQARELLERVSECLNKDRCSTFD 702
Query: 833 DCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQF 892
DCI+WARLKFEDYF+NRVKQL +TFPEDAATS GAPFWSAPKRFP L FS ADQSHL F
Sbjct: 703 DCISWARLKFEDYFSNRVKQLTFTFPEDAATSMGAPFWSAPKRFPRALQFSAADQSHLNF 762
Query: 893 VLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASV 952
+++A+ILRAES+ ++IP+W K+ KL D V+++ VP F PK+ IVTDEKA++LS+ SV
Sbjct: 763 IMSASILRAESFGVAIPEWAKDTSKLADVVNKIAVPTFEPKQGVNIVTDEKASNLSSTSV 822
Query: 953 DDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEV 1012
DD AVI DL+ KL++ + L GF+MKPIQFEKDDDTN+HMDLI+GLANMRARNYSIPEV
Sbjct: 823 DDVAVIEDLLAKLQEYAKMLLPGFQMKPIQFEKDDDTNFHMDLISGLANMRARNYSIPEV 882
Query: 1013 DKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAE 1072
DKLKAKFIAGRIIPAIATSTAMATGLVCLELYKV+ G H VEDYRNTFANLALPLFSMAE
Sbjct: 883 DKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVIAGEHPVEDYRNTFANLALPLFSMAE 942
Query: 1073 PVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRH 1132
PVPPKV+KH++ SWTVWDRW ++ N TL EL++W +KGL AYSISCG+ LLYN+MF RH
Sbjct: 943 PVPPKVMKHKETSWTVWDRWSVQGNLTLAELLQWFADKGLTAYSISCGTSLLYNNMFARH 1002
Query: 1133 KDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
KDR+ KKVVD+AR++AKV++P YRRHLD+ VACED+++ND+DIPLVSVYF
Sbjct: 1003 KDRLTKKVVDIAREVAKVDVPEYRRHLDIGVACEDEDENDVDIPLVSVYF 1052
BLAST of Carg23870-RA vs. ExPASy TrEMBL
Match:
A0A6J1G6A0 (E1 ubiquitin-activating enzyme OS=Cucurbita moschata OX=3662 GN=LOC111451218 PE=3 SV=1)
HSP 1 Score: 2200.2 bits (5700), Expect = 0.0e+00
Identity = 1092/1092 (100.00%), Postives = 1092/1092 (100.00%), Query Frame = 0
Query: 91 MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 150
MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA
Sbjct: 1 MLPRKRAGEEGAVLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAA 60
Query: 151 VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 210
VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG
Sbjct: 61 VPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG 120
Query: 211 AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 270
AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV
Sbjct: 121 AEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIV 180
Query: 271 HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 330
HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF
Sbjct: 181 HTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDF 240
Query: 331 GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 390
GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG
Sbjct: 241 GPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDG 300
Query: 391 KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 450
KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF
Sbjct: 301 KPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDF 360
Query: 451 SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK 510
SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK
Sbjct: 361 SKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPK 420
Query: 511 LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 570
LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE
Sbjct: 421 LLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASE 480
Query: 571 FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 630
FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT
Sbjct: 481 FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTIT 540
Query: 631 DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 690
DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS
Sbjct: 541 DDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDS 600
Query: 691 FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 750
FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD
Sbjct: 601 FWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRD 660
Query: 751 PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 810
PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA
Sbjct: 661 PPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDA 720
Query: 811 QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 870
QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW
Sbjct: 721 QSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFW 780
Query: 871 SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF 930
SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF
Sbjct: 781 SAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDF 840
Query: 931 MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 990
MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN
Sbjct: 841 MPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTN 900
Query: 991 YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 1050
YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG
Sbjct: 901 YHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGG 960
Query: 1051 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1110
HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK
Sbjct: 961 HKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNK 1020
Query: 1111 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1170
GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED
Sbjct: 1021 GLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDED 1080
Query: 1171 NDIDIPLVSVYF 1183
NDIDIPLVSVYF
Sbjct: 1081 NDIDIPLVSVYF 1092
BLAST of Carg23870-RA vs. ExPASy TrEMBL
Match:
A0A6J1KZ04 (E1 ubiquitin-activating enzyme OS=Cucurbita maxima OX=3661 GN=LOC111499539 PE=3 SV=1)
HSP 1 Score: 2172.1 bits (5627), Expect = 0.0e+00
Identity = 1081/1095 (98.72%), Postives = 1087/1095 (99.27%), Query Frame = 0
Query: 91 MLPRKRAGEEGAVLEQQIDNSS---SNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNV 150
MLPRKRAGEEGAVLEQQIDNSS SNISNSVQNAGVSLIKKHRIDNCNVDSNVN NTNV
Sbjct: 1 MLPRKRAGEEGAVLEQQIDNSSSNISNISNSVQNAGVSLIKKHRIDNCNVDSNVNFNTNV 60
Query: 151 AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 210
AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ
Sbjct: 61 AAAVPTGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQ 120
Query: 211 GLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS 270
GLGAEIAKNVILAGVKSVTLHD+GVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS
Sbjct: 121 GLGAEIAKNVILAGVKSVTLHDDGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNS 180
Query: 271 VIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF 330
VIVHTL TKLV+EQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF
Sbjct: 181 VIVHTLTTKLVKEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVF 240
Query: 331 CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL 390
CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL
Sbjct: 241 CDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTEL 300
Query: 391 NDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL 450
NDGKPRRIKNCRAYSFTLDEDTTN+GIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL
Sbjct: 301 NDGKPRRIKNCRAYSFTLDEDTTNYGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLL 360
Query: 451 SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDI 510
SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMN+SLGDGRVEDI
Sbjct: 361 SDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNESLGDGRVEDI 420
Query: 511 NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD 570
NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD
Sbjct: 421 NPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALD 480
Query: 571 ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL 630
ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL
Sbjct: 481 ASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKL 540
Query: 631 TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF 690
TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF
Sbjct: 541 TITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVF 600
Query: 691 NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA 750
NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA
Sbjct: 601 NDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGA 660
Query: 751 SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA 810
SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA
Sbjct: 661 SRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNA 720
Query: 811 GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA 870
GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA
Sbjct: 721 GDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGA 780
Query: 871 PFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIV 930
PFWSAPKRFPHPLPFS ADQSHLQFVLAAAILRAESYAISIPDWVKNPRKL DAVDRVIV
Sbjct: 781 PFWSAPKRFPHPLPFSTADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLADAVDRVIV 840
Query: 931 PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDD 990
PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLV+KLEDTRRNLPEGFRMKPIQFEKDD
Sbjct: 841 PDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVDKLEDTRRNLPEGFRMKPIQFEKDD 900
Query: 991 DTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL 1050
DTNYHMDLIAGLANMRARN+SIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL
Sbjct: 901 DTNYHMDLIAGLANMRARNFSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVL 960
Query: 1051 DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL 1110
DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL
Sbjct: 961 DGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWL 1020
Query: 1111 KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACED 1170
KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPY RHLDVVVACED
Sbjct: 1021 KNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYCRHLDVVVACED 1080
Query: 1171 DEDNDIDIPLVSVYF 1183
DEDNDIDIPLVSVYF
Sbjct: 1081 DEDNDIDIPLVSVYF 1095
BLAST of Carg23870-RA vs. ExPASy TrEMBL
Match:
A0A0A0KE12 (E1 ubiquitin-activating enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G181590 PE=3 SV=1)
HSP 1 Score: 2069.7 bits (5361), Expect = 0.0e+00
Identity = 1040/1157 (89.89%), Postives = 1076/1157 (93.00%), Query Frame = 0
Query: 48 FHSSSKKPLHSSSILSF----------HSPSPDSFLPFFL------SLCYAPNRSLLHFM 107
F SSS SSSI F H P SF P F+ S SLLHFM
Sbjct: 7 FLSSSSSSSTSSSISPFLFFEKPLDSHHIPHCSSFNPPFIRFLPSSSFTVLIGTSLLHFM 66
Query: 108 LPRKRAGEEGAVLEQQIDNSSSNI-----SNSVQNAGVSLIKKHRIDNCNVDSNVNSNTN 167
LPRKRAGEEG V+E++ DN S+++ ++SVQN G S IKK RID+ DSN N+N
Sbjct: 67 LPRKRAGEEGVVVEEETDNGSTSVVINNNNSSVQNVGASFIKKQRIDS---DSNSNTNVA 126
Query: 168 VAAAVP-TGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISG 227
A VP T +NIVND ASLIMASGN NP DIDEDLHSRQLAVYGRETMRKLFASNVLISG
Sbjct: 127 AVATVPTTASNIVNDAASLIMASGNSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLISG 186
Query: 228 MQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLN 287
MQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESD+GKNRALASAQKLQDLN
Sbjct: 187 MQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDVGKNRALASAQKLQDLN 246
Query: 288 NSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGS 347
NSVIVHTL +KLV+EQLS FEVVVFTDT LDKAMEFNDFCHNHQPPISFIKSEVRGLFGS
Sbjct: 247 NSVIVHTLTSKLVKEQLSDFEVVVFTDTSLDKAMEFNDFCHNHQPPISFIKSEVRGLFGS 306
Query: 348 VFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMT 407
VFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMT
Sbjct: 307 VFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMT 366
Query: 408 ELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDF 467
ELNDGKPRRIKNCRAYSFTL+EDTTNFG YEKGGIVTQVKQPKVLNFKPLREAINDPGDF
Sbjct: 367 ELNDGKPRRIKNCRAYSFTLEEDTTNFGSYEKGGIVTQVKQPKVLNFKPLREAINDPGDF 426
Query: 468 LLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVE 527
LLSDFSKFDRPPLIHLAFLALDKFVTELGR PVAGSE+DAQKLISVASN+N+SLGDGRVE
Sbjct: 427 LLSDFSKFDRPPLIHLAFLALDKFVTELGRLPVAGSEEDAQKLISVASNINESLGDGRVE 486
Query: 528 DINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEA 587
DINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTE+
Sbjct: 487 DINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTES 546
Query: 588 LDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEG 647
LDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCS+EG
Sbjct: 547 LDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSSEG 606
Query: 648 KLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETEN 707
KLTITDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AAVAINRHLNIEALQNRVSPETEN
Sbjct: 607 KLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAAAAAVAINRHLNIEALQNRVSPETEN 666
Query: 708 VFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENY 767
VF+DSFWENLSV+VNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENY
Sbjct: 667 VFDDSFWENLSVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENY 726
Query: 768 GASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMM 827
GASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP+DVNAYLSNPSEY S+MM
Sbjct: 727 GASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPSEYTSAMM 786
Query: 828 NAGDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSN 887
NAGDAQSRDTLER+LECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDA TSN
Sbjct: 787 NAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAQTSN 846
Query: 888 GAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRV 947
GAPFWSAPKRFPHPL FS +DQSHLQFVLAAAILR+ESYAI IPDWVKNPRKL DA+DR+
Sbjct: 847 GAPFWSAPKRFPHPLQFSTSDQSHLQFVLAAAILRSESYAIPIPDWVKNPRKLADAIDRI 906
Query: 948 IVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEK 1007
IVPDFMPKKDAKIVTDEKATSLS ASVDDAAVIHDL KLE+T R LPEGFRMKPIQFEK
Sbjct: 907 IVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLAGKLEETCRKLPEGFRMKPIQFEK 966
Query: 1008 DDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK 1067
DDD+N+HMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK
Sbjct: 967 DDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYK 1026
Query: 1068 VLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIK 1127
VLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI
Sbjct: 1027 VLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELID 1086
Query: 1128 WLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVAC 1183
WLKNKGLNAYSISCGSCLLYNSMFPRH+DRMDKKVVDLARD+AKVELPPYRRHLDVVVAC
Sbjct: 1087 WLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPPYRRHLDVVVAC 1146
BLAST of Carg23870-RA vs. ExPASy TrEMBL
Match:
A0A5A7VQ32 (E1 ubiquitin-activating enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold615G00120 PE=3 SV=1)
HSP 1 Score: 2068.9 bits (5359), Expect = 0.0e+00
Identity = 1025/1117 (91.76%), Postives = 1064/1117 (95.26%), Query Frame = 0
Query: 72 FLPFFLSLCYAPNRSLLHFMLPRKRAGEEGAVLEQQIDNSSSNI-----SNSVQNAGVSL 131
F+ F + C+ SLLHFMLPRKRAGEEG V+E++ DN S+++ ++SVQN G SL
Sbjct: 38 FVSFLILRCFGIFSSLLHFMLPRKRAGEEGVVVEEETDNGSTSVVFNNNNSSVQNVGASL 97
Query: 132 IKKHRIDNCNVDSNVNSNTNVAAAVP-TGNNIVNDGASLIMASGNLNPQDIDEDLHSRQL 191
IKK RID+ DSN N+N A VP T NNIVND ASLIMASGN NP DIDEDLHSRQL
Sbjct: 98 IKKQRIDS---DSNSNTNVAAVATVPTTANNIVNDAASLIMASGNSNPPDIDEDLHSRQL 157
Query: 192 AVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFS 251
AVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFS
Sbjct: 158 AVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFS 217
Query: 252 ESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFC 311
ESD+GKNRALASAQKLQDLNNSVIVHTL TKLV+EQLS FEVVVFTDT LDKAMEF+DFC
Sbjct: 218 ESDVGKNRALASAQKLQDLNNSVIVHTLTTKLVKEQLSDFEVVVFTDTSLDKAMEFDDFC 277
Query: 312 HNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDD 371
HNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDD
Sbjct: 278 HNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDD 337
Query: 372 ERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVK 431
ERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTL+EDT NFG YEKGGIVTQVK
Sbjct: 338 ERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLEEDTANFGSYEKGGIVTQVK 397
Query: 432 QPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDA 491
QPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGR P+AGSE+DA
Sbjct: 398 QPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVTELGRLPIAGSEEDA 457
Query: 492 QKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGK 551
QKLISVAS +N+SLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGK
Sbjct: 458 QKLISVASTINESLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGK 517
Query: 552 FHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGA 611
FHPLVQFFYFDSVESLPTE+LDAS+FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGA
Sbjct: 518 FHPLVQFFYFDSVESLPTESLDASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGA 577
Query: 612 LGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVA 671
LGCEFLKNLALMGVSCS+EGKLTITDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AAVA
Sbjct: 578 LGCEFLKNLALMGVSCSSEGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAAAAAVA 637
Query: 672 INRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLES 731
INRHLNIEALQNRVSPETENVF+DSFWENLSV+VNALDNVNARLYVDQRCLYFQKPLLES
Sbjct: 638 INRHLNIEALQNRVSPETENVFDDSFWENLSVVVNALDNVNARLYVDQRCLYFQKPLLES 697
Query: 732 GTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEK 791
GTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEK
Sbjct: 698 GTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEK 757
Query: 792 TPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDY 851
TP+DVNAYLSNP+EY S+MMNAGDAQSRDTLER+LECLDRERCETFEDCITWARLKFEDY
Sbjct: 758 TPTDVNAYLSNPNEYTSAMMNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDY 817
Query: 852 FANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYA 911
FANRVKQLIYTFPEDA TSNGAPFWSAPKRFPHPL FS +DQSHLQFVLAAAILRAESYA
Sbjct: 818 FANRVKQLIYTFPEDAQTSNGAPFWSAPKRFPHPLQFSTSDQSHLQFVLAAAILRAESYA 877
Query: 912 ISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKL 971
I +PDWVKNPRKL DA+DRVIVPDFMPKKDAKIVTDEKATSLS ASVDDAAVIHDL KL
Sbjct: 878 IPVPDWVKNPRKLADAIDRVIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLAGKL 937
Query: 972 EDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRII 1031
E+TRR LPEGFRMKPIQFEKDDD+N+HMDLIAGLANMRARNYSIPEVDKLKAKFIAGRII
Sbjct: 938 EETRRKLPEGFRMKPIQFEKDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRII 997
Query: 1032 PAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMS 1091
PAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMS
Sbjct: 998 PAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMS 1057
Query: 1092 WTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLAR 1151
WTVWDRWIIKDNPTLRELI WLKNKGLNAYSISCGSCLLYNSMFPRH+DRMDKKVVDLAR
Sbjct: 1058 WTVWDRWIIKDNPTLRELIDWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLAR 1117
Query: 1152 DIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
D+AKVELP YRRHLDVVVACEDDEDNDIDIPLVSVYF
Sbjct: 1118 DVAKVELPLYRRHLDVVVACEDDEDNDIDIPLVSVYF 1151
BLAST of Carg23870-RA vs. ExPASy TrEMBL
Match:
A0A1S3CNI4 (E1 ubiquitin-activating enzyme OS=Cucumis melo OX=3656 GN=LOC103502928 PE=3 SV=1)
HSP 1 Score: 2054.3 bits (5321), Expect = 0.0e+00
Identity = 1017/1098 (92.62%), Postives = 1053/1098 (95.90%), Query Frame = 0
Query: 91 MLPRKRAGEEGAVLEQQIDNSSSNI-----SNSVQNAGVSLIKKHRIDNCNVDSNVNSNT 150
MLPRKRAGEEG V+E++ DN S+++ ++SVQN G SLIKK RID+ DSN N+N
Sbjct: 1 MLPRKRAGEEGVVVEEETDNGSTSVVFNNNNSSVQNVGASLIKKQRIDS---DSNSNTNV 60
Query: 151 NVAAAVP-TGNNIVNDGASLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLIS 210
A VP T NNIVND ASLIMASGN NP DIDEDLHSRQLAVYGRETMRKLFASNVLIS
Sbjct: 61 AAVATVPTTANNIVNDAASLIMASGNSNPPDIDEDLHSRQLAVYGRETMRKLFASNVLIS 120
Query: 211 GMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDL 270
GMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESD+GKNRALASAQKLQDL
Sbjct: 121 GMQGLGAEIAKNVILAGVKSVTLHDEGVVELWDLSSNFVFSESDVGKNRALASAQKLQDL 180
Query: 271 NNSVIVHTLPTKLVEEQLSKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFG 330
NNSVIVHTL TKLV+EQLS FEVVVFTDT LDKAMEF+DFCHNHQPPISFIKSEVRGLFG
Sbjct: 181 NNSVIVHTLTTKLVKEQLSDFEVVVFTDTSLDKAMEFDDFCHNHQPPISFIKSEVRGLFG 240
Query: 331 SVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGM 390
SVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGM
Sbjct: 241 SVFCDFGPEFTVYDVYGEDPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGM 300
Query: 391 TELNDGKPRRIKNCRAYSFTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGD 450
TELNDGKPRRIKNCRAYSFTL+EDT NFG YEKGGIVTQVKQPKVLNFKPLREAINDPGD
Sbjct: 301 TELNDGKPRRIKNCRAYSFTLEEDTANFGSYEKGGIVTQVKQPKVLNFKPLREAINDPGD 360
Query: 451 FLLSDFSKFDRPPLIHLAFLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRV 510
FLLSDFSKFDRPPLIHLAFLALDKFVTELGR P+AGSE+DAQKLISVAS +N+SLGDGRV
Sbjct: 361 FLLSDFSKFDRPPLIHLAFLALDKFVTELGRLPIAGSEEDAQKLISVASTINESLGDGRV 420
Query: 511 EDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTE 570
EDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTE
Sbjct: 421 EDINPKLLRHFAFGAKAVLNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTE 480
Query: 571 ALDASEFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNE 630
+LDAS+FRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCS+E
Sbjct: 481 SLDASDFRPLNSRYDAQISVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSSE 540
Query: 631 GKLTITDDDVIEKSNLSRQFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETE 690
GKLTITDDDVIEKSNLSRQFLFRDWNIGQ KSTVAA+AAVAINRHLNIEALQNRVSPETE
Sbjct: 541 GKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAAAAAVAINRHLNIEALQNRVSPETE 600
Query: 691 NVFNDSFWENLSVIVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTEN 750
NVF+DSFWENLSV+VNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTEN
Sbjct: 601 NVFDDSFWENLSVVVNALDNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTEN 660
Query: 751 YGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSM 810
YGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTP+DVNAYLSNP+EY S+M
Sbjct: 661 YGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTDVNAYLSNPNEYTSAM 720
Query: 811 MNAGDAQSRDTLERVLECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATS 870
MNAGDAQSRDTLER+LECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDA TS
Sbjct: 721 MNAGDAQSRDTLERILECLDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAQTS 780
Query: 871 NGAPFWSAPKRFPHPLPFSPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDR 930
NGAPFWSAPKRFPHPL FS +DQSHLQFVLAAAILRAESYAI +PDWVKNPRKL DA+DR
Sbjct: 781 NGAPFWSAPKRFPHPLQFSTSDQSHLQFVLAAAILRAESYAIPVPDWVKNPRKLADAIDR 840
Query: 931 VIVPDFMPKKDAKIVTDEKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFE 990
VIVPDFMPKKDAKIVTDEKATSLS ASVDDAAVIHDL KLE+TRR LPEGFRMKPIQFE
Sbjct: 841 VIVPDFMPKKDAKIVTDEKATSLSTASVDDAAVIHDLAGKLEETRRKLPEGFRMKPIQFE 900
Query: 991 KDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELY 1050
KDDD+N+HMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELY
Sbjct: 901 KDDDSNFHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELY 960
Query: 1051 KVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI 1110
KVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI
Sbjct: 961 KVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI 1020
Query: 1111 KWLKNKGLNAYSISCGSCLLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVA 1170
WLKNKGLNAYSISCGSCLLYNSMFPRH+DRMDKKVVDLARD+AKVELP YRRHLDVVVA
Sbjct: 1021 DWLKNKGLNAYSISCGSCLLYNSMFPRHRDRMDKKVVDLARDVAKVELPLYRRHLDVVVA 1080
Query: 1171 CEDDEDNDIDIPLVSVYF 1183
CEDDEDNDIDIPLVSVYF
Sbjct: 1081 CEDDEDNDIDIPLVSVYF 1095
BLAST of Carg23870-RA vs. TAIR 10
Match:
AT2G30110.1 (ubiquitin-activating enzyme 1 )
HSP 1 Score: 1726.5 bits (4470), Expect = 0.0e+00
Identity = 834/1080 (77.22%), Postives = 956/1080 (88.52%), Query Frame = 0
Query: 103 VLEQQIDNSSSNISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGA 162
+L ++ ++ N++ + ++ KK RID S+ S++ +A+ G + +
Sbjct: 1 MLHKRASEANDKNDNTIIGSDLASSKKRRIDFTESSSD-KSSSILASGSSRGFHGDSVVQ 60
Query: 163 SLIMASGNLNPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGV 222
+ MA GN N Q+IDEDLHSRQLAVYGRETMR+LFASNVLISGM GLGAEIAKN+ILAGV
Sbjct: 61 QIDMAFGNSNRQEIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEIAKNLILAGV 120
Query: 223 KSVTLHDEGVVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQL 282
KSVTLHDE VVELWDLSSNFVFSE D+GKNRA AS QKLQDLNN+V+V +L L +E L
Sbjct: 121 KSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSSLTKSLNKEDL 180
Query: 283 SKFEVVVFTDTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGE 342
S F+VVVF+D +++A+EF+D+CH+HQPPI+F+K++VRGLFGSVFCDFGPEF V DV GE
Sbjct: 181 SGFQVVVFSDISMERAIEFDDYCHSHQPPIAFVKADVRGLFGSVFCDFGPEFAVLDVDGE 240
Query: 343 DPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYS 402
+PHTGIIASISN+N A +SCVDDERLEF+DGDLVVFSEV GMTELNDGKPR+IK+ R YS
Sbjct: 241 EPHTGIIASISNENQAFISCVDDERLEFEDGDLVVFSEVEGMTELNDGKPRKIKSTRPYS 300
Query: 403 FTLDEDTTNFGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLA 462
FTLDEDTTN+G Y KGGIVTQVKQPK+LNFKPLREA+ DPGDFL SDFSKFDRPPL+HLA
Sbjct: 301 FTLDEDTTNYGTYVKGGIVTQVKQPKLLNFKPLREALKDPGDFLFSDFSKFDRPPLLHLA 360
Query: 463 FLALDKFVTELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAV 522
F ALD F E GRFPVAGSE+DAQKLIS+A+ +N GD +VE+++ KLLRHF+FGAKAV
Sbjct: 361 FQALDHFKAEAGRFPVAGSEEDAQKLISIATAINTGQGDLKVENVDQKLLRHFSFGAKAV 420
Query: 523 LNPMAAMFGGIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQI 582
LNPMAAMFGGIV QEV+KACSGKFHPL QFFYFDSVESLP+E +D+S+F P NSRYDAQI
Sbjct: 421 LNPMAAMFGGIVGQEVVKACSGKFHPLFQFFYFDSVESLPSEPVDSSDFAPRNSRYDAQI 480
Query: 583 SVFGSKLQKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSR 642
SVFG+K QKKLE+AKVF VGSGALGCEFLKNLALMGVSC ++GKLT+TDDD+IEKSNLSR
Sbjct: 481 SVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGVSCGSQGKLTVTDDDIIEKSNLSR 540
Query: 643 QFLFRDWNIGQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNAL 702
QFLFRDWNIGQ KSTVAASAA IN NIEALQNRV ETENVF+D+FWENL+V+VNAL
Sbjct: 541 QFLFRDWNIGQAKSTVAASAAAVINPRFNIEALQNRVGAETENVFDDAFWENLTVVVNAL 600
Query: 703 DNVNARLYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVH 762
DNVNARLYVD RCLYFQKPLLESGTLG KCNTQ VIPHLTENYGASRDPPEKQAPMCTVH
Sbjct: 601 DNVNARLYVDSRCLYFQKPLLESGTLGTKCNTQSVIPHLTENYGASRDPPEKQAPMCTVH 660
Query: 763 SFPHNIDHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLEC 822
SFPHNIDHCLTWARSEFEGLLEKTP++VNAYLS+P EY +SMM+AGDAQ+RDTLER++EC
Sbjct: 661 SFPHNIDHCLTWARSEFEGLLEKTPAEVNAYLSSPVEYTNSMMSAGDAQARDTLERIVEC 720
Query: 823 LDRERCETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPF 882
L++E+CETF+DC+TWARL+FEDYF NRVKQLIYTFPEDAATS GAPFWSAPKRFP PL +
Sbjct: 721 LEKEKCETFQDCLTWARLRFEDYFVNRVKQLIYTFPEDAATSTGAPFWSAPKRFPRPLQY 780
Query: 883 SPADQSHLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDE 942
S +D S L F+ A AILRAE++ I IP+W KNP++ +AVDRVIVPDF P++DAKIVTDE
Sbjct: 781 SSSDPSLLNFITATAILRAETFGIPIPEWTKNPKEAAEAVDRVIVPDFEPRQDAKIVTDE 840
Query: 943 KATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANM 1002
KAT+L+ ASVDDAAVI DL+ K++ R NL FRMKPIQFEKDDDTNYHMD+IAGLANM
Sbjct: 841 KATTLTTASVDDAAVIDDLIAKIDQCRHNLSPDFRMKPIQFEKDDDTNYHMDVIAGLANM 900
Query: 1003 RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFAN 1062
RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVE YRNTFAN
Sbjct: 901 RARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEAYRNTFAN 960
Query: 1063 LALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSC 1122
LALPLFSMAEP+PPKV+KHRDM+WTVWDRW++K NPTLRE+++WL++KGL+AYSISCGSC
Sbjct: 961 LALPLFSMAEPLPPKVVKHRDMAWTVWDRWVLKGNPTLREVLQWLEDKGLSAYSISCGSC 1020
Query: 1123 LLYNSMFPRHKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1182
LL+NSMF RHK+RMDKKVVDLARD+AKVELPPYR HLDVVVACED++DND+DIPLVS+YF
Sbjct: 1021 LLFNSMFTRHKERMDKKVVDLARDVAKVELPPYRNHLDVVVACEDEDDNDVDIPLVSIYF 1079
BLAST of Carg23870-RA vs. TAIR 10
Match:
AT5G06460.1 (ubiquitin activating enzyme 2 )
HSP 1 Score: 1694.1 bits (4386), Expect = 0.0e+00
Identity = 822/1071 (76.75%), Postives = 933/1071 (87.11%), Query Frame = 0
Query: 115 ISNSVQNAGVSLIKKHRIDNCNVDSNVNSNTNVAAAVPTGNNIVNDGASLIMA---SGNL 174
+ ++ + S +KK RID+ N + ++++ N+I + + MA + N
Sbjct: 6 VKENIIASASSPMKKRRIDHTESADGSAINASNSSSIGLNNSIGGNDTVMSMAEFGNDNS 65
Query: 175 NPQDIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGAEIAKNVILAGVKSVTLHDEG 234
N Q+IDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLG EIAKN+ILAGVKSVTLHDE
Sbjct: 66 NNQEIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGVEIAKNIILAGVKSVTLHDEN 125
Query: 235 VVELWDLSSNFVFSESDIGKNRALASAQKLQDLNNSVIVHTLPTKLVEEQLSKFEVVVFT 294
VVELWDLSSNFVF+E DIGKNRALAS KLQ+LNN+V V TL KL +EQLS F+VVVF
Sbjct: 126 VVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLTGKLTKEQLSDFQVVVFV 185
Query: 295 DTGLDKAMEFNDFCHNHQPPISFIKSEVRGLFGSVFCDFGPEFTVYDVYGEDPHTGIIAS 354
D +KA E +D+CH+HQPPI+FIK++VRGLFGS+FCDFGP FTV DV GE+PH+GIIAS
Sbjct: 186 DISFEKATEIDDYCHSHQPPIAFIKADVRGLFGSLFCDFGPHFTVLDVDGEEPHSGIIAS 245
Query: 355 ISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRRIKNCRAYSFTLDEDTTN 414
+SN+NP VSCVDDERLEF+DG+LVVFSEV GMTELNDGKPR+IKN + +SFTL+EDT++
Sbjct: 246 VSNENPGFVSCVDDERLEFEDGNLVVFSEVEGMTELNDGKPRKIKNVKPFSFTLEEDTSS 305
Query: 415 FGIYEKGGIVTQVKQPKVLNFKPLREAINDPGDFLLSDFSKFDRPPLIHLAFLALDKFVT 474
+G Y KGGIVTQVKQPKVLNFKPLREA+ DPGDFLLSDFSKFDRPPL+HLAF ALD+F +
Sbjct: 306 YGQYMKGGIVTQVKQPKVLNFKPLREALKDPGDFLLSDFSKFDRPPLLHLAFQALDRFSS 365
Query: 475 ELGRFPVAGSEDDAQKLISVASNMNKSLGDGRVEDINPKLLRHFAFGAKAVLNPMAAMFG 534
+ GRFP AGSE+DAQKL+ +A ++N+ LGD R+ED+N KLLRH AFG++AVLNPMAAMFG
Sbjct: 366 QAGRFPFAGSEEDAQKLVEIAVDINEGLGDARLEDVNSKLLRHLAFGSRAVLNPMAAMFG 425
Query: 535 GIVAQEVLKACSGKFHPLVQFFYFDSVESLPTEALDASEFRPLNSRYDAQISVFGSKLQK 594
GIV QEV+KACSGKFHP+ QFFYFDSVESLP E LDASEFRP NSRYDAQISVFGS LQK
Sbjct: 426 GIVGQEVVKACSGKFHPIFQFFYFDSVESLPKEPLDASEFRPQNSRYDAQISVFGSTLQK 485
Query: 595 KLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDWNI 654
KLE+A+VF+VG+GALGCEFLKNLALMGVSC +GKLT+TDDDVIEKSNLSRQFLFRDWNI
Sbjct: 486 KLEDARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQFLFRDWNI 545
Query: 655 GQNKSTVAASAAVAINRHLNIEALQNRVSPETENVFNDSFWENLSVIVNALDNVNARLYV 714
GQ KSTVAA+AA IN LNI+ALQNRV PETENVF+DSFWENL+V+VNALDNV ARLYV
Sbjct: 546 GQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENLTVVVNALDNVTARLYV 605
Query: 715 DQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC 774
D RC+YFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC
Sbjct: 606 DSRCVYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHC 665
Query: 775 LTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERCETF 834
LTWARSEFEGLLEKTP++VNAYLS+P EY +M AGDAQ+RDTL RV+ECL++E+C +F
Sbjct: 666 LTWARSEFEGLLEKTPAEVNAYLSDPVEYMKAMRTAGDAQARDTLGRVVECLEKEKCNSF 725
Query: 835 EDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQSHLQ 894
+DCITWARL+FEDYFANRVKQL YTFPEDAATS GAPFWSAPKRFP PL FS D SH+
Sbjct: 726 QDCITWARLRFEDYFANRVKQLCYTFPEDAATSTGAPFWSAPKRFPRPLQFSSTDLSHIN 785
Query: 895 FVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLSAAS 954
FV+AA+ILRAE++ I P+W K L +AV+RVIVPDF PKKDA IVTDEKAT+LS AS
Sbjct: 786 FVMAASILRAETFGIPTPEWAKTRAGLAEAVERVIVPDFEPKKDATIVTDEKATTLSTAS 845
Query: 955 VDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYSIPE 1014
VDDAAVI +L KL R +L FRMK IQFEKDDDTNYHMD+IAGLANMRARNYS+PE
Sbjct: 846 VDDAAVIDELNAKLVRCRMSLQPEFRMKAIQFEKDDDTNYHMDMIAGLANMRARNYSVPE 905
Query: 1015 VDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMA 1074
VDKLKAKFIAGRIIPAIATSTAMATG VCLE+YKVLDG HKVEDYRNTFANLALPLFSMA
Sbjct: 906 VDKLKAKFIAGRIIPAIATSTAMATGFVCLEMYKVLDGSHKVEDYRNTFANLALPLFSMA 965
Query: 1075 EPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELIKWLKNKGLNAYSISCGSCLLYNSMFPR 1134
EPVPPKV+KH+D SWTVWDRW+++ NPTLREL+ WLK KGLNAYSISCGS LLYNSMF R
Sbjct: 966 EPVPPKVVKHQDQSWTVWDRWVMRGNPTLRELLDWLKEKGLNAYSISCGSSLLYNSMFSR 1025
Query: 1135 HKDRMDKKVVDLARDIAKVELPPYRRHLDVVVACEDDEDNDIDIPLVSVYF 1183
HK+RM+++VVDLARD+A VELP YRRH+DVVVACEDD D D+DIPLVSVYF
Sbjct: 1026 HKERMNRRVVDLARDVAGVELPAYRRHVDVVVACEDDNDADVDIPLVSVYF 1076
BLAST of Carg23870-RA vs. TAIR 10
Match:
AT2G21470.3 (SUMO-activating enzyme 2 )
HSP 1 Score: 164.9 bits (416), Expect = 4.1e-40
Identity = 161/552 (29.17%), Postives = 244/552 (44.20%), Query Frame = 0
Query: 590 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 649
Q ++ AKV MVG+G +GCE LK LAL G + I D D IE SNL+RQFLFR
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66
Query: 650 NIGQNKSTVAASAAVAINRHLNIEALQNRV-SPETENVFNDSFWENLSVIVNALDNVNAR 709
++GQ+K+ VA A + ++NI + V +PE F+ F++ V++N LDN++AR
Sbjct: 67 HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126
Query: 710 LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 769
+V++ CL PL+ESGT G + I TE Y P K P+CT+ S P
Sbjct: 127 RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186
Query: 770 DHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERC 829
HC+ WA+ + L K D N N S+ + ++ D ER +
Sbjct: 187 VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246
Query: 830 ETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHP------LPF 889
ED + R ++ F + + +AA SN + + +R P P LP
Sbjct: 247 ---EDIEQYGRKIYDHVFGSNI---------EAALSNEETWKN--RRRPRPIYSKDVLPE 306
Query: 890 SPADQSHLQFVLAAAILRAESYAISIPD-WVKNPRKLGDAVDRVIVPDFMPKKDAKIVTD 949
S Q+ ++ + ++P +KNP++L +V F+
Sbjct: 307 SLTQQN--GSTQNCSVTDGDLMVSAMPSLGLKNPQELWGLTQNSLV--FI---------- 366
Query: 950 EKATSLSAASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLAN 1009
+A L A V + + F+KDD ++ + AN
Sbjct: 367 -EALKLFFAKRKKVCV-------------------EIGHLTFDKDD--QLAVEFVTAAAN 426
Query: 1010 MRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFA 1069
+RA ++ IP +AK IAG I+ A+AT+ A+ GL+ +E KVL V+ +R T+
Sbjct: 427 IRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAIKVLK--KDVDKFRMTYC 481
Query: 1070 ------NLALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI-KWLKNK-GLN 1126
L L EP P + S T I LR+L+ K +K K G+N
Sbjct: 487 LEHPSKKLLLMPIEPYEPNPACYV----CSETPLVLEINTRKSKLRDLVDKIVKTKLGMN 481
BLAST of Carg23870-RA vs. TAIR 10
Match:
AT2G21470.1 (SUMO-activating enzyme 2 )
HSP 1 Score: 163.7 bits (413), Expect = 9.0e-40
Identity = 159/545 (29.17%), Postives = 241/545 (44.22%), Query Frame = 0
Query: 590 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 649
Q ++ AKV MVG+G +GCE LK LAL G + I D D IE SNL+RQFLFR
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66
Query: 650 NIGQNKSTVAASAAVAINRHLNIEALQNRV-SPETENVFNDSFWENLSVIVNALDNVNAR 709
++GQ+K+ VA A + ++NI + V +PE F+ F++ V++N LDN++AR
Sbjct: 67 HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126
Query: 710 LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 769
+V++ CL PL+ESGT G + I TE Y P K P+CT+ S P
Sbjct: 127 RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186
Query: 770 DHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERC 829
HC+ WA+ + L K D N N S+ + ++ D ER +
Sbjct: 187 VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246
Query: 830 ETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQS 889
ED + R ++ F + + +AA SN + + +R P P+
Sbjct: 247 ---EDIEQYGRKIYDHVFGSNI---------EAALSNEETWKN--RRRPRPI-------- 306
Query: 890 HLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLS 949
+ + VL ++ + + D ++ MP K L
Sbjct: 307 YSKDVLPESLTQQNGSTQNC-----------SVTDGDLMVSAMPSLGLK-----NPQELW 366
Query: 950 AASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYS 1009
+ + I L KL +R G + F+KDD ++ + AN+RA ++
Sbjct: 367 GLTQNSLVFIEAL--KLFFAKRKKEIGH----LTFDKDD--QLAVEFVTAAANIRAESFG 426
Query: 1010 IPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFA------N 1069
IP +AK IAG I+ A+AT+ A+ GL+ +E KVL V+ +R T+
Sbjct: 427 IPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAIKVLK--KDVDKFRMTYCLEHPSKK 478
Query: 1070 LALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI-KWLKNK-GLNAYSISCG 1126
L L EP P + S T I LR+L+ K +K K G+N I G
Sbjct: 487 LLLMPIEPYEPNPACYV----CSETPLVLEINTRKSKLRDLVDKIVKTKLGMNLPLIMHG 478
BLAST of Carg23870-RA vs. TAIR 10
Match:
AT2G21470.2 (SUMO-activating enzyme 2 )
HSP 1 Score: 163.7 bits (413), Expect = 9.0e-40
Identity = 159/545 (29.17%), Postives = 241/545 (44.22%), Query Frame = 0
Query: 590 QKKLENAKVFMVGSGALGCEFLKNLALMGVSCSNEGKLTITDDDVIEKSNLSRQFLFRDW 649
Q ++ AKV MVG+G +GCE LK LAL G + I D D IE SNL+RQFLFR
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLKTLALSGFE-----DIHIIDMDTIEVSNLNRQFLFRRS 66
Query: 650 NIGQNKSTVAASAAVAINRHLNIEALQNRV-SPETENVFNDSFWENLSVIVNALDNVNAR 709
++GQ+K+ VA A + ++NI + V +PE F+ F++ V++N LDN++AR
Sbjct: 67 HVGQSKAKVARDAVLRFRPNINIRSYHANVKNPE----FDVDFFKQFDVVLNGLDNLDAR 126
Query: 710 LYVDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNI 769
+V++ CL PL+ESGT G + I TE Y P K P+CT+ S P
Sbjct: 127 RHVNRLCLAADVPLVESGTTGFLGQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKF 186
Query: 770 DHCLTWARSEFEGLLEKTPSDVNAYLSNPSEYASSMMNAGDAQSRDTLERVLECLDRERC 829
HC+ WA+ + L K D N N S+ + ++ D ER +
Sbjct: 187 VHCIVWAK---DLLFAKLFGDKNQ--DNDLNVRSNNSASSSKETEDVFERSED------- 246
Query: 830 ETFEDCITWARLKFEDYFANRVKQLIYTFPEDAATSNGAPFWSAPKRFPHPLPFSPADQS 889
ED + R ++ F + + +AA SN + + +R P P+
Sbjct: 247 ---EDIEQYGRKIYDHVFGSNI---------EAALSNEETWKN--RRRPRPI-------- 306
Query: 890 HLQFVLAAAILRAESYAISIPDWVKNPRKLGDAVDRVIVPDFMPKKDAKIVTDEKATSLS 949
+ + VL ++ + + D ++ MP K L
Sbjct: 307 YSKDVLPESLTQQNGSTQNC-----------SVTDGDLMVSAMPSLGLK-----NPQELW 366
Query: 950 AASVDDAAVIHDLVNKLEDTRRNLPEGFRMKPIQFEKDDDTNYHMDLIAGLANMRARNYS 1009
+ + I L KL +R G + F+KDD ++ + AN+RA ++
Sbjct: 367 GLTQNSLVFIEAL--KLFFAKRKKEIGH----LTFDKDD--QLAVEFVTAAANIRAESFG 426
Query: 1010 IPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKVLDGGHKVEDYRNTFA------N 1069
IP +AK IAG I+ A+AT+ A+ GL+ +E KVL V+ +R T+
Sbjct: 427 IPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAIKVLK--KDVDKFRMTYCLEHPSKK 478
Query: 1070 LALPLFSMAEPVPPKVIKHRDMSWTVWDRWIIKDNPTLRELI-KWLKNK-GLNAYSISCG 1126
L L EP P + S T I LR+L+ K +K K G+N I G
Sbjct: 487 LLLMPIEPYEPNPACYV----CSETPLVLEINTRKSKLRDLVDKIVKTKLGMNLPLIMHG 478
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7035346.1 | 0.0e+00 | 100.00 | Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. argyros... | [more] |
KAG6605397.1 | 0.0e+00 | 99.47 | Ubiquitin-activating enzyme E1 1, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
XP_022947321.1 | 0.0e+00 | 100.00 | ubiquitin-activating enzyme E1 1-like [Cucurbita moschata] >XP_022947322.1 ubiqu... | [more] |
XP_023532141.1 | 0.0e+00 | 98.99 | ubiquitin-activating enzyme E1 1-like [Cucurbita pepo subsp. pepo] >XP_023532142... | [more] |
XP_023006875.1 | 0.0e+00 | 98.72 | ubiquitin-activating enzyme E1 1-like [Cucurbita maxima] >XP_023006876.1 ubiquit... | [more] |
Match Name | E-value | Identity | Description | |
P93028 | 0.0e+00 | 77.22 | Ubiquitin-activating enzyme E1 1 OS=Arabidopsis thaliana OX=3702 GN=UBA1 PE=1 SV... | [more] |
P92974 | 0.0e+00 | 76.75 | Ubiquitin-activating enzyme E1 2 OS=Arabidopsis thaliana OX=3702 GN=UBA2 PE=1 SV... | [more] |
P20973 | 0.0e+00 | 77.25 | Ubiquitin-activating enzyme E1 1 OS=Triticum aestivum OX=4565 GN=UBA1 PE=1 SV=1 | [more] |
P31251 | 0.0e+00 | 77.15 | Ubiquitin-activating enzyme E1 2 OS=Triticum aestivum OX=4565 GN=UBA2 PE=2 SV=1 | [more] |
P31252 | 0.0e+00 | 74.85 | Ubiquitin-activating enzyme E1 3 OS=Triticum aestivum OX=4565 GN=UBA3 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1G6A0 | 0.0e+00 | 100.00 | E1 ubiquitin-activating enzyme OS=Cucurbita moschata OX=3662 GN=LOC111451218 PE=... | [more] |
A0A6J1KZ04 | 0.0e+00 | 98.72 | E1 ubiquitin-activating enzyme OS=Cucurbita maxima OX=3661 GN=LOC111499539 PE=3 ... | [more] |
A0A0A0KE12 | 0.0e+00 | 89.89 | E1 ubiquitin-activating enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G181590 PE=3 S... | [more] |
A0A5A7VQ32 | 0.0e+00 | 91.76 | E1 ubiquitin-activating enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |
A0A1S3CNI4 | 0.0e+00 | 92.62 | E1 ubiquitin-activating enzyme OS=Cucumis melo OX=3656 GN=LOC103502928 PE=3 SV=1 | [more] |