Carg22842-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22842-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Dipeptide epimerase
Location	Carg_Chr15: 401597 .. 402959 (-)
Sequence length	708
RNA-Seq Expression	Carg22842-RA
Synteny	Carg22842-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGATATGGGGATAGTTCCTCTTGTTTTTGAGCAACCTGTAGACCGAGATGACTGGAAAGGCCTTCGCCAAGTCAGCAACGTTGCTAGAACATATGGGATACCTGTTGCAGTGGATGAAAGCTGCCGGAGTTTGAGTGACGTTCAGAAGATCATCGATGAAAATCTGGTGGATGCCATAAACATCAAGTTGCCCAAGTTTGGAGTCCTTGGAGTTCTAGAAATAACAAATCTGGCAAGAAAATCAGGCCTGACTCTTATGGTCGACAGCATGGCCGAGACGAGACTCGCAACCGGCTTTGCAGGCCATTTGGCTGCTGGAGTTGGTTGCTTCAAGTAATTTTTTATGTCTTTTTTTATCACCATTCTGCTAAGAGCCCTGGCTTGAGTGAAGTTCCTTATTCAAATTGCAGGTACATTGTTCTTGATACACCATTTTTATTAGCAGAAGATCCTGTTGTTGGAGGCTATGAAGGTAAACATTCTATAAACCAAAATGAACTTTCTTGCTTACCATGACGGCGCCTGAGTCTAAGCTCTACTTTTGTCCTCAACTAGTTTCTGGTGCTGTGTACAAGTTCAATAATGCAAGAGGCCAAGGAGGGTTCTTGAATTGGGATCTTCTTCCTGGTGAGATATACTGCCCATGTGTTCTTCTTATATCATCTTTCTCAGTCTTACTTTCAGCCATTTCATTGTTTACAGCCATTTGAATATCCTGCTATGTCTTACCAAACCAACCAAATGGATGTGCACCATATTTACTTCTTAAAAATATCCCCAATATACTTGGTTACAAGAGATTCCTTATGAGCCCTGTGATACCCCTCACCCGACTTTATCCAAGCAGATGTTGTCCACTTTTAACCGGTTACGTAGTGCTGTCAGTTTCACAATTTTAAAACACATCTGTTAGGGAGAGGTTTCCACGTCCTTATAAGAAATGGTTCGTTCCCCCGTTTCCCCGTTCCCCCTATCCAACCGATGTGAGATCTCACAACTTAGCACTGACTTATAAAACTAAATTTGCTACCGGACACTATGTAAATTTGAAATGATTTTATGATAGAAATCAAACTTTGTGTGGAAGTGTATACTAATTCTGTGGCTGATATTATTCTGCAGAAGCTGGTGGGTTACCTTAAATCCTTCAACTTTTGGCGGCCCTTATGGTTTTCTGTAGAAAATAAATGCTCCAGTTAGTCTAAATTGATGCATGGCACTCTGCAAATGGCTAAGCCTCGAAGGAGTAGAGGACAAACTTCGTTGTTGTTATGAACAAAACCTTTTAAGCTATCAATTACTAGGCCATGGAAATTGGAGAAAATATGTACATTATAATAAGCTTTTTTTTTTTTTGT

mRNA sequence

ATGGCAGATATGGGGATAGTTCCTCTTGTTTTTGAGCAACCTGTAGACCGAGATGACTGGAAAGGCCTTCGCCAAGTCAGCAACGTTGCTAGAACATATGGGATACCTGTTGCAGTGGATGAAAGCTGCCGGAGTTTGAGTGACGTTCAGAAGATCATCGATGAAAATCTGGTGGATGCCATAAACATCAAGTTGCCCAAGTTTGGAGTCCTTGGAGTTCTAGAAATAACAAATCTGGCAAGAAAATCAGGCCTGACTCTTATGGTCGACAGCATGGCCGAGACGAGACTCGCAACCGGCTTTGCAGGCCATTTGGCTGCTGGAGTTGGTTGCTTCAAGTACATTGTTCTTGATACACCATTTTTATTAGCAGAAGATCCTGTTGTTGGAGGCTATGAAGTTTCTGGTGCTGTGTACAAGTTCAATAATGCAAGAGGCCAAGGAGGGTTCTTGAATTGGGATCTTCTTCCTGAAGCTGGTGGGTTACCTTAAATCCTTCAACTTTTGGCGGCCCTTATGGTTTTCTGTAGAAAATAAATGCTCCAGTTAGTCTAAATTGATGCATGGCACTCTGCAAATGGCTAAGCCTCGAAGGAGTAGAGGACAAACTTCGTTGTTGTTATGAACAAAACCTTTTAAGCTATCAATTACTAGGCCATGGAAATTGGAGAAAATATGTACATTATAATAAGCTTTTTTTTTTTTTGT

Coding sequence (CDS)

ATGGCAGATATGGGGATAGTTCCTCTTGTTTTTGAGCAACCTGTAGACCGAGATGACTGGAAAGGCCTTCGCCAAGTCAGCAACGTTGCTAGAACATATGGGATACCTGTTGCAGTGGATGAAAGCTGCCGGAGTTTGAGTGACGTTCAGAAGATCATCGATGAAAATCTGGTGGATGCCATAAACATCAAGTTGCCCAAGTTTGGAGTCCTTGGAGTTCTAGAAATAACAAATCTGGCAAGAAAATCAGGCCTGACTCTTATGGTCGACAGCATGGCCGAGACGAGACTCGCAACCGGCTTTGCAGGCCATTTGGCTGCTGGAGTTGGTTGCTTCAAGTACATTGTTCTTGATACACCATTTTTATTAGCAGAAGATCCTGTTGTTGGAGGCTATGAAGTTTCTGGTGCTGTGTACAAGTTCAATAATGCAAGAGGCCAAGGAGGGTTCTTGAATTGGGATCTTCTTCCTGAAGCTGGTGGGTTACCTTAA

Protein sequence

MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDAINIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTPFLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP

Homology

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BLAST of Carg22842-RA vs. NCBI nr
Match: KAG7015927.1 (L-Ala-D/L-amino acid epimerase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 330.9 bits (847), Expect = 6.3e-87
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA
Sbjct: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP
Sbjct: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120

Query: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP 164
           FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP
Sbjct: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP 163

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BLAST of Carg22842-RA vs. NCBI nr
Match: XP_023550334.1 (L-Ala-D/L-amino acid epimerase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 326.6 bits (836), Expect = 1.2e-85
Identity = 160/163 (98.16%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           + DMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSL+DVQKIIDENLVDA
Sbjct: 293 LKDMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLTDVQKIIDENLVDA 352

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP
Sbjct: 353 INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 412

Query: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP 164
           FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP
Sbjct: 413 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP 455

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BLAST of Carg22842-RA vs. NCBI nr
Match: KAG6578347.1 (L-Ala-D/L-amino acid epimerase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 326.6 bits (836), Expect = 1.2e-85
Identity = 160/163 (98.16%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           + DMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSL+DVQKIIDENLVDA
Sbjct: 291 LKDMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLTDVQKIIDENLVDA 350

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP
Sbjct: 351 INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 410

Query: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP 164
           FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP
Sbjct: 411 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP 453

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BLAST of Carg22842-RA vs. NCBI nr
Match: XP_022938632.1 (L-Ala-D/L-amino acid epimerase-like [Cucurbita moschata])

HSP 1 Score: 323.9 bits (829), Expect = 7.7e-85
Identity = 159/162 (98.15%), Postives = 161/162 (99.38%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           + DMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSL+DVQKIIDENLVDA
Sbjct: 269 LKDMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLTDVQKIIDENLVDA 328

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP
Sbjct: 329 INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 388

Query: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGL 163
           FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGL
Sbjct: 389 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGL 430

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BLAST of Carg22842-RA vs. NCBI nr
Match: XP_038884241.1 (L-Ala-D/L-amino acid epimerase-like [Benincasa hispida])

HSP 1 Score: 305.8 bits (782), Expect = 2.2e-79
Identity = 149/163 (91.41%), Postives = 155/163 (95.09%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           + DMG+VPLVFEQPVDRDDWKGL +VSNVAR YGIPVAVDESCRSL+DVQKIIDENLVDA
Sbjct: 284 LKDMGVVPLVFEQPVDRDDWKGLHEVSNVARMYGIPVAVDESCRSLTDVQKIIDENLVDA 343

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           INIKLPKFGVLGVLEI  LARKSGL LMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP
Sbjct: 344 INIKLPKFGVLGVLEIIKLARKSGLILMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 403

Query: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP 164
           FLLAED VVGGYE SGAVYKFNNARGQGGFLNW+LLP+AGGLP
Sbjct: 404 FLLAEDHVVGGYEASGAVYKFNNARGQGGFLNWNLLPDAGGLP 446

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BLAST of Carg22842-RA vs. ExPASy Swiss-Prot
Match: B9I2J6 (L-Ala-D/L-amino acid epimerase OS=Populus trichocarpa OX=3694 GN=POPTR_0012s05040g PE=3 SV=2)

HSP 1 Score: 206.1 bits (523), Expect = 3.1e-52
Identity = 100/152 (65.79%), Postives = 119/152 (78.29%), Query Frame = 0


Query: 4   MGIVPLVFEQPVDRDDWKGLRQVSNVAR-TYGIPVAVDESCRSLSDVQKIIDENLVDAIN 63
           MG+ P++FEQPV RDDW+GL  V+++A+  YG+ VA DESCRSL D ++II  NL D IN
Sbjct: 267 MGVTPILFEQPVHRDDWEGLGHVTHIAKGKYGVSVAADESCRSLVDAKRIIKGNLADVIN 326

Query: 64  IKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTPFL 123
           IKL K GV+G LEI   AR SGL LM+  M ETRLA GFAGHLAAG GCFK+I LDTP L
Sbjct: 327 IKLAKVGVVGGLEIIEEARTSGLDLMIGGMVETRLAMGFAGHLAAGFGCFKFIDLDTPLL 386

Query: 124 LAEDPVVGGYEVSGAVYKFNNARGQGGFLNWD 155
           L+EDPV+ GYEVSGAVYKF +A+G  GFL+WD
Sbjct: 387 LSEDPVLEGYEVSGAVYKFTDAQGHAGFLDWD 418

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BLAST of Carg22842-RA vs. ExPASy Swiss-Prot
Match: A9B055 (Aromatic dipeptide epimerase OS=Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95) OX=316274 GN=Haur_1114 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 4.6e-24
Identity = 62/143 (43.36%), Postives = 86/143 (60.14%), Query Frame = 0


Query: 7   VPLV-FEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDAINIKL 66
           +P+V FEQP+ R+DW G+ QV+  +   G  VA DES RS  DV +I  E     INIKL
Sbjct: 213 IPMVLFEQPLPREDWAGMAQVTAQS---GFAVAADESARSAHDVLRIAREGTASVINIKL 272

Query: 67  PKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTPFLLAE 126
            K GV   L++  +A+ +GL LM+  M E+ LA  F+ +LAAG G F +I LDTP  +AE
Sbjct: 273 MKAGVAEGLKMIAIAQAAGLGLMIGGMVESILAMSFSANLAAGNGGFDFIDLDTPLFIAE 332

Query: 127 DPVVGGYEVSGAVYKFNNARGQG 149
            P +GG+  +G   +  +  G G
Sbjct: 333 HPFIGGFAQTGGTLQLADVAGHG 352

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BLAST of Carg22842-RA vs. ExPASy Swiss-Prot
Match: B5EFW2 (Hydrophobic dipeptide epimerase OS=Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) OX=404380 GN=Gbem_2417 PE=3 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.4e-20
Identity = 51/123 (41.46%), Postives = 72/123 (58.54%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           +A  G+ P++ EQPV + DW GL +++         V  DES  SL+D ++ ID N V A
Sbjct: 214 LAAKGVRPVLLEQPVPKMDWDGLSEITAALTGSETLVCADESVGSLADARRAIDSNAVSA 273

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           IN+K  K G+L   EI  LA   G+ LM+ +M E+ LA   + H AAG+ CF Y+ +DT 
Sbjct: 274 INVKFMKSGILEGAEIARLAASRGIRLMLGAMMESALAVTASAHFAAGLACFDYLDMDTT 333

Query: 121 FLL 124
           F L
Sbjct: 334 FFL 336

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BLAST of Carg22842-RA vs. ExPASy Swiss-Prot
Match: Q9WXM1 (L-Ala-D/L-Glu epimerase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_0006 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 9.4e-17
Identity = 52/132 (39.39%), Postives = 73/132 (55.30%), Query Frame = 0


Query: 5   GIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDAINIK 64
           GI   V+EQPV R+D +GL+ V   +     PVA DES R+  DV +++ E  VD +NIK
Sbjct: 209 GIDIAVYEQPVRREDIEGLKFVRFHS---PFPVAADESARTKFDVMRLVKEEAVDYVNIK 268

Query: 65  LPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTPFLLA 124
           L K G+   L I  +A  SGL LM+  M E+ L    + H A G G F++  LD+  +L 
Sbjct: 269 LMKSGISDALAIVEIAESSGLKLMIGCMGESSLGINQSVHFALGTGAFEFHDLDSHLMLK 328

Query: 125 EDPVVGGYEVSG 137
           E+   G +   G
Sbjct: 329 EEVFRGKFIQDG 337

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BLAST of Carg22842-RA vs. ExPASy Swiss-Prot
Match: O34508 (L-Ala-D/L-Glu epimerase OS=Bacillus subtilis (strain 168) OX=224308 GN=ykfB PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.1e-12
Identity = 50/149 (33.56%), Postives = 69/149 (46.31%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           M D G+   + EQPV +DD  GL++V++   T   P+  DES  +     +++     D 
Sbjct: 208 MEDAGLGIELVEQPVHKDDLAGLKKVTDATDT---PIMADESVFTPRQAFEVLQTRSADL 267

Query: 61  INIKLPKF-GVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDT 120
           INIKL K  G+ G  +I  +A   G+  MV SM ET+L    A H AA          D 
Sbjct: 268 INIKLMKAGGISGAEKINAMAEACGVECMVGSMIETKLGITAAAHFAASKRNITRFDFDA 327

Query: 121 PFLLAEDPVVGGYEVSGAVYKFNNARGQG 149
           P +L  D   GG   SG+        G G
Sbjct: 328 PLMLKTDVFNGGITYSGSTISMPGKPGLG 353

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BLAST of Carg22842-RA vs. ExPASy TrEMBL
Match: A0A6J1FEM1 (L-Ala-D/L-amino acid epimerase-like OS=Cucurbita moschata OX=3662 GN=LOC111444808 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 3.7e-85
Identity = 159/162 (98.15%), Postives = 161/162 (99.38%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           + DMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSL+DVQKIIDENLVDA
Sbjct: 269 LKDMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLTDVQKIIDENLVDA 328

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP
Sbjct: 329 INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 388

Query: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGL 163
           FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGL
Sbjct: 389 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGL 430

[Close]

BLAST of Carg22842-RA vs. ExPASy TrEMBL
Match: A0A0A0LQR8 (MR_MLE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G404880 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.4e-79
Identity = 148/163 (90.80%), Postives = 155/163 (95.09%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           + D+GIVPLVFEQPVDRDDWKGL +VSNVARTYGIPVAVDESCRSL+DV KIID+NLVDA
Sbjct: 281 LKDVGIVPLVFEQPVDRDDWKGLHEVSNVARTYGIPVAVDESCRSLTDVWKIIDKNLVDA 340

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           INIKLPKFGVLG LEI NLARKSGL LMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP
Sbjct: 341 INIKLPKFGVLGALEIINLARKSGLILMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 400

Query: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP 164
            LLAEDPVVGGYE SGAVYKFNNARGQGGFLNW+LLP+AGGLP
Sbjct: 401 LLLAEDPVVGGYEASGAVYKFNNARGQGGFLNWNLLPDAGGLP 443

[Close]

BLAST of Carg22842-RA vs. ExPASy TrEMBL
Match: A0A6J1BXC6 (L-Ala-D/L-amino acid epimerase-like OS=Momordica charantia OX=3673 GN=LOC111006196 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.6e-75
Identity = 140/163 (85.89%), Postives = 149/163 (91.41%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           + DMGIVPLVFEQPV RDDWKGLR+VSN+AR YG+PVA DESCR+ +DV+KIIDENLVDA
Sbjct: 327 LKDMGIVPLVFEQPVARDDWKGLREVSNLAREYGVPVAADESCRNSTDVRKIIDENLVDA 386

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           INIKLPKFGVLGVLEI  L RKSGL LM+DSMAETRL TGFAGHLAAG GCFKYIVLD P
Sbjct: 387 INIKLPKFGVLGVLEIIELERKSGLILMIDSMAETRLGTGFAGHLAAGSGCFKYIVLDAP 446

Query: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP 164
           FLLAEDPVVGGYE SGAVYKFNNARGQGGFLNWDLLP+AGG P
Sbjct: 447 FLLAEDPVVGGYEASGAVYKFNNARGQGGFLNWDLLPDAGGSP 489

[Close]

BLAST of Carg22842-RA vs. ExPASy TrEMBL
Match: A0A1S3B326 (L-Ala-D/L-amino acid epimerase-like OS=Cucumis melo OX=3656 GN=LOC103485448 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.6e-75
Identity = 143/163 (87.73%), Postives = 150/163 (92.02%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           + D+GIVPLVFEQPVDRDDWKGL++VSNVAR +GIPVAVDESCRSL+DV KIIDENLVDA
Sbjct: 273 LKDVGIVPLVFEQPVDRDDWKGLQEVSNVARMHGIPVAVDESCRSLTDVCKIIDENLVDA 332

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           INIKLPKFGVLG LEI NLARKSGL LMVDSMAETRL TGFAGHLAAGVGCFKYIVLDTP
Sbjct: 333 INIKLPKFGVLGALEIINLARKSGLILMVDSMAETRLGTGFAGHLAAGVGCFKYIVLDTP 392

Query: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLPEAGGLP 164
            LLAEDPVVGGYE SGAVYKFNNARGQGGFLNW+LLP A   P
Sbjct: 393 LLLAEDPVVGGYEASGAVYKFNNARGQGGFLNWNLLPCASQRP 435

[Close]

BLAST of Carg22842-RA vs. ExPASy TrEMBL
Match: A0A5A7SRY5 (MuDRA-like transposase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold7507G00080 PE=4 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.0e-74
Identity = 141/157 (89.81%), Postives = 148/157 (94.27%), Query Frame = 0


Query: 1   MADMGIVPLVFEQPVDRDDWKGLRQVSNVARTYGIPVAVDESCRSLSDVQKIIDENLVDA 60
           + D+GIVPLVFEQPVDRDDWKGL++VSNVAR +GIPVAVDESCRSL+DV KIIDENLVDA
Sbjct: 273 LKDVGIVPLVFEQPVDRDDWKGLQEVSNVARMHGIPVAVDESCRSLTDVCKIIDENLVDA 332

Query: 61  INIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTP 120
           INIKLPKFGVLG LEI NLARKSGL LMVDSMAETRL TGFAGHLAAGVGCFKYIVLDTP
Sbjct: 333 INIKLPKFGVLGALEIINLARKSGLILMVDSMAETRLGTGFAGHLAAGVGCFKYIVLDTP 392

Query: 121 FLLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWDLLP 158
            LLAEDPVVGGYE SGAVYKFNNARGQGGFLNW+LLP
Sbjct: 393 LLLAEDPVVGGYEASGAVYKFNNARGQGGFLNWNLLP 429

[Close]

BLAST of Carg22842-RA vs. TAIR 10
Match: AT3G18270.1 (cytochrome P450, family 77, subfamily A, polypeptide 5 pseudogene )

HSP 1 Score: 190.7 bits (483), Expect = 9.5e-49
Identity = 86/153 (56.21%), Postives = 117/153 (76.47%), Query Frame = 0


Query: 3   DMGIVPLVFEQPVDRDDWKGLRQVSNVART-YGIPVAVDESCRSLSDVQKIIDENLVDAI 62
           +M + P++FEQPV RD+W+GL  V+  A+  +G+ +A DESCR L+D++KII+ N+VD +
Sbjct: 254 EMKVTPVLFEQPVHRDNWEGLSHVTRTAKNRFGVSIAADESCRGLTDLKKIIEGNIVDVV 313

Query: 63  NIKLPKFGVLGVLEITNLARKSGLTLMVDSMAETRLATGFAGHLAAGVGCFKYIVLDTPF 122
           NIKL K G+L  LE+  LAR SG+ LM+  M ETRLA GF+GHLAAG+GCF++I LDTP 
Sbjct: 314 NIKLAKTGILESLEVIELARSSGIELMIGGMVETRLAMGFSGHLAAGLGCFRFIDLDTPL 373

Query: 123 LLAEDPVVGGYEVSGAVYKFNNARGQGGFLNWD 155
           LLA+DPV GGY+  GAVY+F +  G GG+L W+
Sbjct: 374 LLADDPVQGGYKACGAVYEFKDEGGHGGYLQWN 406

The following BLAST results are available for this feature:

BLAST of Carg22842-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015927.1	6.3e-87	100.00	L-Ala-D/L-amino acid epimerase, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_023550334.1	1.2e-85	98.16	L-Ala-D/L-amino acid epimerase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6578347.1	1.2e-85	98.16	L-Ala-D/L-amino acid epimerase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022938632.1	7.7e-85	98.15	L-Ala-D/L-amino acid epimerase-like [Cucurbita moschata]	[more]
XP_038884241.1	2.2e-79	91.41	L-Ala-D/L-amino acid epimerase-like [Benincasa hispida]	[more]

BLAST of Carg22842-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9I2J6	3.1e-52	65.79	L-Ala-D/L-amino acid epimerase OS=Populus trichocarpa OX=3694 GN=POPTR_0012s0504...	[more]
A9B055	4.6e-24	43.36	Aromatic dipeptide epimerase OS=Herpetosiphon aurantiacus (strain ATCC 23779 / D...	[more]
B5EFW2	1.4e-20	41.46	Hydrophobic dipeptide epimerase OS=Geobacter bemidjiensis (strain Bem / ATCC BAA...	[more]
Q9WXM1	9.4e-17	39.39	L-Ala-D/L-Glu epimerase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / J...	[more]
O34508	1.1e-12	33.56	L-Ala-D/L-Glu epimerase OS=Bacillus subtilis (strain 168) OX=224308 GN=ykfB PE=1...	[more]

BLAST of Carg22842-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FEM1	3.7e-85	98.15	L-Ala-D/L-amino acid epimerase-like OS=Cucurbita moschata OX=3662 GN=LOC11144480...	[more]
A0A0A0LQR8	2.4e-79	90.80	MR_MLE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G404880 PE=4...	[more]
A0A6J1BXC6	1.6e-75	85.89	L-Ala-D/L-amino acid epimerase-like OS=Momordica charantia OX=3673 GN=LOC1110061...	[more]
A0A1S3B326	4.6e-75	87.73	L-Ala-D/L-amino acid epimerase-like OS=Cucumis melo OX=3656 GN=LOC103485448 PE=4...	[more]
A0A5A7SRY5	1.0e-74	89.81	MuDRA-like transposase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold7...	[more]

BLAST of Carg22842-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18270.1	9.5e-49	56.21	cytochrome P450, family 77, subfamily A, polypeptide 5 pseudogene	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029065	Enolase C-terminal domain-like	PFAM	PF13378	MR_MLE_C	coord: 8..148 e-value: 1.1E-18 score: 67.7
IPR036849	Enolase-like, C-terminal domain superfamily	GENE3D	3.20.20.120		coord: 1..152 e-value: 2.3E-31 score: 110.8
IPR036849	Enolase-like, C-terminal domain superfamily	SUPERFAMILY	51604	Enolase C-terminal domain-like	coord: 8..157
None	No IPR available	PANTHER	PTHR48073:SF4	DIPEPTIDE EPIMERASE	coord: 2..156
None	No IPR available	PANTHER	PTHR48073	O-SUCCINYLBENZOATE SYNTHASE-RELATED	coord: 2..156

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg22842	Carg22842	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22842-RA:4	Carg22842-RA:4	exon
Carg22842-RA:3	Carg22842-RA:3	exon
Carg22842-RA:2	Carg22842-RA:2	exon
Carg22842-RA:1	Carg22842-RA:1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22842-RA:three_prime_utr	Carg22842-RA:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22842-RA:cds	Carg22842-RA:cds_4	CDS
Carg22842-RA:cds	Carg22842-RA:cds_3	CDS
Carg22842-RA:cds	Carg22842-RA:cds_2	CDS
Carg22842-RA:cds	Carg22842-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg22842-RA	Carg22842-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016855	racemase and epimerase activity, acting on amino acids and derivatives
molecular_function	GO:0008270	zinc ion binding