Carg22159-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22159-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	OTU domain-containing protein
Location	Carg_Chr08: 2253202 .. 2256237 (+)
Sequence length	1158
RNA-Seq Expression	Carg22159-RA
Synteny	Carg22159-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGACATTTGTCTTGGTTCGTCTCGTCCCCATTGCCCTAGGTATCTCAGTTCCGTTTCTTCTTCCTTTGTTCTTCTGTTCGGGCTAGCCCTAGCTGTTGATGCACTGCGCTTCGTATTCGATTTTGACGGGAATTTGTTGATAGCCGAAGAAGGAAGCTTCGTGTGGGTCGTTAATTCAGTTAATCTTGGAGGAGAAGATAGTTGTCGAGGTAAAGTTTGGCTTCTGAATCCATTATGTGATTTGTTTCTAATTACAGTCTCCCAATCCCTGGCGTCTCTATCTCAAAACAGAGGGAAATTTGAATTTTTATTTTCATGTTTTGGTATTTTGGTTGGCTGTTGGCAATGTTAGTGGAAATTGACGAAATGAGTTTTGCCTCTAGTATTTGGAGTCGGTTCTTGATCGTGGTTGTAGTTTTACGGCATGTTAGAAAGGTATGATAAGACATTCTTTAGATCATGATGCCGCTGGTTTGAGAAATTGATTGGTTGTTGTTTTATTTCATTAGATTGTTCATTGAAGGACATATTGCGCTCTTCTTAAGCATCTGACTGACTAACTTGCCCCTATCTCGACAATCTCAAAAGAACGAACTCCACTCGTGGGATTTTAGCTTAATCCATTCTCCTGAAGTCTCTTTACCTGTACTTAGATTTCATAAATCCTTATTGAAAGTCTAAAGACGTTTGACGTTGGAATCCATGAATCTTTGTTCCTAGTGTCTCGTTATCTTCTGGTTCCAGAACATTTTCTTGGCTTCAATTATATTTGTTCGTATGGAAGTAATTTAAGGCTTTGCTGAAGATTTTCGCTCAAAATAATATGGGAAGGGGCGAGTGAGAGTGGTTATTCATTTTGTTAAGTGGGTGTGAGTTTCTAGAATGTGTGAGGGGAGGGGATTTGAGATTGGCAATTAAAGTCAGCCCTTATTGTTGTATTTCTGAAGAATATAACGTTTCGGTAAAATCATGTCTACATTAGATAGACATAAAATGCCTCAAACTATACTAGAGAGAGTTGTGACTAGTTATCAATTCTTCCTACCTGGAGTTATATGCTATCTAAACCGTTGTGTTGCCTTTTTTGCTTGCTAACCTATAACAGCTTCTGTAGGCTATACCTGTAGTTCCTAGGGAATGGTTGTAACTTCAGCGCACTATAATTTCAATTTGAAACAGTTTCTAATTGGTACTTCTCCAATTTTCTTTGTGAGCAATGTTGAGGGAAATATTCATAACATCAAATGAGCGGTTCATCAATTGTTAATTCTTAATCGGCCCTGCTTCCCACAAGTTTTTAGTTCTTAATTACATTACGAAAAAAATCCATCAACAATTTCCCCCCATACAAGGCATCAATTTCCACATTTATGAGTACTAATAACATTAAATTCATGCTGATAAATTGTGAGGAGAATTCACTTGTTGAAGTAATATTTTTTAGACTAATCAGCTCTTTAACTAGAGAAGTTGAATTGAAGCTTTTGATGAGTTACGTATTCTGTTTCTGGCTTTTATATCAGGAATATGGAGGACGCATCATCTGAAACTATCACAGATAAAGTATCTCAAGAGAATCATGAAACTCGTGATGAAATGCTTTCTAGGCACAGGTAAATAGAAATTTGACTCTTGGCTTCTTTTCTATCATTTTTTTTTTTCCTCAGTTTCGAGTATAGCCTGTTGTTTCAATGTTTGCTGCCACTGGTGTTTAATGTAATCGAATTAGAAAAATATGTATATTTTTGTTTGTTTTCACCCCACAAAGAGTTGGTTACTTGATATATAGATTCAGCTTGATTTTCATGAAGTTTTAAAATGTTCATTAGAACTTTAAGATATTAGTCATCTAGGGGGGCAATGTATTAAAAACATTTCAAGATCCGGTTCCTCAAATCGATTCTTGTAATCTTACAAATACTAGATTTTTGTGCCATTTCAACTTCAGATGCTTAAAATATGCATATTTTTCCATGTTCAATCACTTCCGAACGAAAGCTTTTAGTATAATCCATATAATTACTACAATATAAGCGTGTGTACTTACTGATGCCTTTGGTACAATTGGTGGTATTATTTACTTTTGTGGTTTACCACCGCGACTTCAATCGAATGGGGAGGGTATCATTAACGTTTTGTGTCTGTATTGCTTGTTGAATTACTGTATCTGCATGCAGGAAAGAGATATCACAGTTGCAGAATAAAGAAATGGAACTTAAAAAGGCCGCGGCTAGAGGTAGCAAAGCTGAACAAAAAATGAAGAAAAAACAGGTGGAGGAAGAGATATCTCAACTTTCTGCGAAGCTAAAAAAGAAACATAATGAAGAACTTGCGTTCTTAGGCTATAGTAGCAGTAATGGAAATGAACGAAGCAATCTCGACAACCTAGTGAAGGCTGTGGCTGGCGTATCGATGACAGCTCAATCCGACCATTCAAAGCCAAGCAAAAGCACAAAACGGAGGGAGAAGAGGGCTCAACAAGAAGCAGCAAGAGACAGAAGAATTCAAGAGGAGCAGAGTAACACTGTGAGTGATAGAATGATCGAGAATGAACAATTGGAAAAGAAGCTTGGACCTCTTGGTTTGACTGTTAATGAAATCAAGCCAGATGGGCATTGCCTCTACAGAGCAGTCGAAAACCAGATGGACTTCCTTTCAGGAGGTTCATCTCCATACAATTACCAAGAACTTCGTCAAATGGTAGCGGCTTACATGAGAGACCATTCAACCGATTACATGCCATTTTTCCTCTCGGAAAACGTCGTAGAAGGTAATTCAAACGAATCGCTGTCCGAAAGGTTTGAAAATTACTGCAAGGAAGTTGAATCCACAGCGGCATGGGGAGGGCAGCTGGAACTTGGTGCTTTAACGCATTGCCTTAAAAAACACATCATGATATTTTCAGGATCCTTCCCTGATGTGGAAATGGGAAAGGAGTACAAATCAGACGGCGTACTAAACTCAAGTATTAGGCTGTCATACCATAAGCATGCCTTTGGACTTGGGGAGCACTACAATTCTGTGGTTCCTGTATGA

mRNA sequence

AGACATTTGTCTTGGTTCGTCTCGTCCCCATTGCCCTAGGTATCTCAGTTCCGTTTCTTCTTCCTTTGTTCTTCTGTTCGGGCTAGCCCTAGCTGTTGATGCACTGCGCTTCGTATTCGATTTTGACGGGAATTTGTTGATAGCCGAAGAAGGAAGCTTCGTGTGGGTCGTTAATTCAGTTAATCTTGGAGGAGAAGATAGTTGTCGAGGAATATGGAGGACGCATCATCTGAAACTATCACAGATAAAGTATCTCAAGAGAATCATGAAACTCGTGATGAAATGCTTTCTAGGCACAGGAAAGAGATATCACAGTTGCAGAATAAAGAAATGGAACTTAAAAAGGCCGCGGCTAGAGGTAGCAAAGCTGAACAAAAAATGAAGAAAAAACAGGTGGAGGAAGAGATATCTCAACTTTCTGCGAAGCTAAAAAAGAAACATAATGAAGAACTTGCGTTCTTAGGCTATAGTAGCAGTAATGGAAATGAACGAAGCAATCTCGACAACCTAGTGAAGGCTGTGGCTGGCGTATCGATGACAGCTCAATCCGACCATTCAAAGCCAAGCAAAAGCACAAAACGGAGGGAGAAGAGGGCTCAACAAGAAGCAGCAAGAGACAGAAGAATTCAAGAGGAGCAGAGTAACACTGTGAGTGATAGAATGATCGAGAATGAACAATTGGAAAAGAAGCTTGGACCTCTTGGTTTGACTGTTAATGAAATCAAGCCAGATGGGCATTGCCTCTACAGAGCAGTCGAAAACCAGATGGACTTCCTTTCAGGAGGTTCATCTCCATACAATTACCAAGAACTTCGTCAAATGGTAGCGGCTTACATGAGAGACCATTCAACCGATTACATGCCATTTTTCCTCTCGGAAAACGTCGTAGAAGGTAATTCAAACGAATCGCTGTCCGAAAGGTTTGAAAATTACTGCAAGGAAGTTGAATCCACAGCGGCATGGGGAGGGCAGCTGGAACTTGGTGCTTTAACGCATTGCCTTAAAAAACACATCATGATATTTTCAGGATCCTTCCCTGATGTGGAAATGGGAAAGGAGTACAAATCAGACGGCGTACTAAACTCAAGTATTAGGCTGTCATACCATAAGCATGCCTTTGGACTTGGGGAGCACTACAATTCTGTGGTTCCTGTATGA

Coding sequence (CDS)

ATGGAGGACGCATCATCTGAAACTATCACAGATAAAGTATCTCAAGAGAATCATGAAACTCGTGATGAAATGCTTTCTAGGCACAGGAAAGAGATATCACAGTTGCAGAATAAAGAAATGGAACTTAAAAAGGCCGCGGCTAGAGGTAGCAAAGCTGAACAAAAAATGAAGAAAAAACAGGTGGAGGAAGAGATATCTCAACTTTCTGCGAAGCTAAAAAAGAAACATAATGAAGAACTTGCGTTCTTAGGCTATAGTAGCAGTAATGGAAATGAACGAAGCAATCTCGACAACCTAGTGAAGGCTGTGGCTGGCGTATCGATGACAGCTCAATCCGACCATTCAAAGCCAAGCAAAAGCACAAAACGGAGGGAGAAGAGGGCTCAACAAGAAGCAGCAAGAGACAGAAGAATTCAAGAGGAGCAGAGTAACACTGTGAGTGATAGAATGATCGAGAATGAACAATTGGAAAAGAAGCTTGGACCTCTTGGTTTGACTGTTAATGAAATCAAGCCAGATGGGCATTGCCTCTACAGAGCAGTCGAAAACCAGATGGACTTCCTTTCAGGAGGTTCATCTCCATACAATTACCAAGAACTTCGTCAAATGGTAGCGGCTTACATGAGAGACCATTCAACCGATTACATGCCATTTTTCCTCTCGGAAAACGTCGTAGAAGGTAATTCAAACGAATCGCTGTCCGAAAGGTTTGAAAATTACTGCAAGGAAGTTGAATCCACAGCGGCATGGGGAGGGCAGCTGGAACTTGGTGCTTTAACGCATTGCCTTAAAAAACACATCATGATATTTTCAGGATCCTTCCCTGATGTGGAAATGGGAAAGGAGTACAAATCAGACGGCGTACTAAACTCAAGTATTAGGCTGTCATACCATAAGCATGCCTTTGGACTTGGGGAGCACTACAATTCTGTGGTTCCTGTATGA

Protein sequence

MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQVEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKSTKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRAVENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENYCKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKHAFGLGEHYNSVVPV

Homology

[Close]

BLAST of Carg22159-RA vs. NCBI nr
Match: KAG6593166.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 597.4 bits (1539), Expect = 7.0e-167
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60
           MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ
Sbjct: 16  MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 75

Query: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120
           VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS
Sbjct: 76  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 135

Query: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180
           TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA
Sbjct: 136 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 195

Query: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240
           VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY
Sbjct: 196 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 255

Query: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300
           CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH
Sbjct: 256 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 315

Query: 301 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVVPV
Sbjct: 316 AFGLGEHYNSVVPV 329

[Close]

BLAST of Carg22159-RA vs. NCBI nr
Match: KAG7025564.1 (OTU domain-containing protein 6B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 597.4 bits (1539), Expect = 7.0e-167
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60
           MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ
Sbjct: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60

Query: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120
           VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS
Sbjct: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120

Query: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180
           TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA
Sbjct: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180

Query: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240
           VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY
Sbjct: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240

Query: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300
           CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH
Sbjct: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300

Query: 301 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVVPV
Sbjct: 301 AFGLGEHYNSVVPV 314

[Close]

BLAST of Carg22159-RA vs. NCBI nr
Match: XP_023514920.1 (OTU domain-containing protein 6B isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.8 bits (1527), Expect = 1.7e-165
Identity = 311/314 (99.04%), Postives = 313/314 (99.68%), Query Frame = 0


Query: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60
           MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQK+KKKQ
Sbjct: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKLKKKQ 60

Query: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120
           VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS
Sbjct: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120

Query: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180
           TKRREKRAQQEAARDRRI+EEQSN VSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA
Sbjct: 121 TKRREKRAQQEAARDRRIEEEQSNIVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180

Query: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240
           VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY
Sbjct: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240

Query: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300
           CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH
Sbjct: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300

Query: 301 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVVPV
Sbjct: 301 AFGLGEHYNSVVPV 314

[Close]

BLAST of Carg22159-RA vs. NCBI nr
Match: XP_023514919.1 (OTU domain-containing protein 6B isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.8 bits (1527), Expect = 1.7e-165
Identity = 311/314 (99.04%), Postives = 313/314 (99.68%), Query Frame = 0


Query: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60
           MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQK+KKKQ
Sbjct: 6   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKLKKKQ 65

Query: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120
           VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS
Sbjct: 66  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 125

Query: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180
           TKRREKRAQQEAARDRRI+EEQSN VSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA
Sbjct: 126 TKRREKRAQQEAARDRRIEEEQSNIVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 185

Query: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240
           VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY
Sbjct: 186 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 245

Query: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300
           CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH
Sbjct: 246 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 305

Query: 301 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVVPV
Sbjct: 306 AFGLGEHYNSVVPV 319

[Close]

BLAST of Carg22159-RA vs. NCBI nr
Match: XP_023004601.1 (OTU domain-containing protein 6B [Cucurbita maxima] >XP_023004602.1 OTU domain-containing protein 6B [Cucurbita maxima] >XP_023004603.1 OTU domain-containing protein 6B [Cucurbita maxima])

HSP 1 Score: 589.7 bits (1519), Expect = 1.5e-164
Identity = 308/314 (98.09%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60
           MEDASSETITDKVSQENHETRDE+LSRHRKEISQLQNKEMELKKAAARGSKAEQK+KKKQ
Sbjct: 1   MEDASSETITDKVSQENHETRDEILSRHRKEISQLQNKEMELKKAAARGSKAEQKLKKKQ 60

Query: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120
           VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVS+TAQSDHSKPSKS
Sbjct: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSLTAQSDHSKPSKS 120

Query: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180
           TKRREKRAQQEAARDRRI+EEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA
Sbjct: 121 TKRREKRAQQEAARDRRIEEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180

Query: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240
           VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY
Sbjct: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240

Query: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300
           CKEVESTAAWGGQLELGALTHCL+KHI+IFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH
Sbjct: 241 CKEVESTAAWGGQLELGALTHCLRKHIIIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300

Query: 301 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVVPV
Sbjct: 301 AFGLGEHYNSVVPV 314

[Close]

BLAST of Carg22159-RA vs. ExPASy Swiss-Prot
Match: Q9LYC7 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 5 OS=Arabidopsis thaliana OX=3702 GN=OTU5 PE=2 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 1.4e-109
Identity = 215/320 (67.19%), Postives = 261/320 (81.56%), Query Frame = 0


Query: 5   SSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQVEEE 64
           S ET  D   ++ HET +EML+RHR+EI QLQNKE E+KKAAA+GSKAEQK KKKQVE++
Sbjct: 12  SEETPMDASQEQQHETVEEMLARHRQEIKQLQNKETEMKKAAAKGSKAEQKAKKKQVEDD 71

Query: 65  ISQLSAKLKKKHNEELAFLGYSSSNGN---------ERSNLDNLVKAVAGVSMTAQSDHS 124
           IS+LS KLK K  +ELA  G+SSS+ N         ++ ++D LV+A+AGVS+TAQ +HS
Sbjct: 72  ISKLSTKLKDKQLKELASQGFSSSSSNNIAKDETTEKKGDIDTLVRAIAGVSVTAQQEHS 131

Query: 125 KPSKSTKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGH 184
           KPSKS KRREKRA++EA R++RI+EEQS+  SDRM+EN +LEKKL PLGLTV+EIKPDGH
Sbjct: 132 KPSKSVKRREKRAKEEADREQRIKEEQSHVKSDRMVENAKLEKKLKPLGLTVSEIKPDGH 191

Query: 185 CLYRAVENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNE-SLS 244
           CLYRAVENQ+   SGG+SPY YQ LR+M A+YMR+H TD++PFFLSE   EG+SN  S  
Sbjct: 192 CLYRAVENQLANRSGGASPYTYQNLREMAASYMREHKTDFLPFFLSE--TEGDSNSGSAE 251

Query: 245 ERFENYCKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIR 304
           ERFE YC+EVESTAAWG QLELGALTHCL+KHI ++SGSFPDVEMGKEY+S    +SS+ 
Sbjct: 252 ERFEKYCREVESTAAWGSQLELGALTHCLRKHIKVYSGSFPDVEMGKEYRSGD--DSSLM 311

Query: 305 LSYHKHAFGLGEHYNSVVPV 315
           LSYH+HAFGLGEHYNSVV V
Sbjct: 312 LSYHRHAFGLGEHYNSVVLV 327

[Close]

BLAST of Carg22159-RA vs. ExPASy Swiss-Prot
Match: Q7ZV00 (Deubiquitinase OTUD6B OS=Danio rerio OX=7955 GN=otud6b PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 8.3e-38
Identity = 116/301 (38.54%), Postives = 163/301 (54.15%), Query Frame = 0


Query: 16  ENHETRDEMLSR-HRKEISQLQNKEMELKKAAARGSKAEQKMKKKQVEEEISQLSAKLKK 75
           E  ET +E L++ HRKE   LQ K   +K A  +      K ++KQ+ E+I++L A+L +
Sbjct: 2   EEVETAEEQLAKQHRKEKKDLQAKIQSMKNAVPK----NDKKRRKQLTEDIAKLEAELSQ 61

Query: 76  KHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKSTKRREKRAQQEAAR 135
           KH  EL     ++S+  E S+  + +        +  S  S+ SK+ KRR+K+A  E  R
Sbjct: 62  KHENELKL--QNTSSVEEVSDALDSMSVANHEEQSDPSKQSRTSKAQKRRDKKAALEKER 121

Query: 136 DRRIQE-EQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRAVENQMDFLSGGSS 195
           + RI E E  N    R  E  +L +KL    L + EI  DGHC+YRAVE+Q   L+    
Sbjct: 122 EMRIAEAEVENLSGSRHQEGLKLREKLVERHLQIKEISSDGHCMYRAVEHQ---LTERGL 181

Query: 196 PYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENYCKEVESTAAWGGQ 255
               +ELR   A YMR H+ D+MPF  + N      +   +E FE YC +V  TAAWGGQ
Sbjct: 182 ALGLKELRDQTAQYMRSHADDFMPFLTNPN----TGDMYTAEEFEKYCSDVADTAAWGGQ 241

Query: 256 LELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKHAFGLGEHYNSVVP 315
           LEL AL+  L+  I +     P + +G+EY         I L Y +HA+GLGEHYNSV P
Sbjct: 242 LELKALSQVLQLPIEVIQADSPCITIGEEYD-----KPKITLIYMRHAYGLGEHYNSVEP 284

[Close]

BLAST of Carg22159-RA vs. ExPASy Swiss-Prot
Match: Q5ZIP6 (Deubiquitinase OTUD6B OS=Gallus gallus OX=9031 GN=OTUD6B PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.9e-37
Identity = 106/293 (36.18%), Postives = 162/293 (55.29%), Query Frame = 0


Query: 23  EMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQVEEEISQLSAKLKKKHNEELAF 82
           +++ R R+E  +LQ K   +K A  +      K ++KQ+ EE+++L A+L++KH EEL  
Sbjct: 17  QLVKRQRREKRELQAKIQGMKNAVPK----NDKKRRKQLAEEVAKLEAELEQKHKEELKQ 76

Query: 83  LGYSSSNGNERSNLDNLVKAVAGVSMTA---QSDHSKPSKSTKRREKRAQQEAARDRRIQ 142
           L  +     E++ +D++   VA   +     Q  H + SK+ KRREK+A  E  R+ RI 
Sbjct: 77  LKEAMP---EQNKIDSIADGVANFELEGREQQIQHPRISKAQKRREKKAALEKEREERIA 136

Query: 143 E-EQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRAVENQMDFLSGGSSPYNYQ 202
           E E  N    R +E+++L   L    L + +I  DGHC+YRA+E+Q   L    + +   
Sbjct: 137 EAEIENLTGARHLESQKLASLLAARHLEIKQIPSDGHCMYRAIEDQ---LKDHHNSWTVA 196

Query: 203 ELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENYCKEVESTAAWGGQLELGA 262
            LR   A Y+  H  D++PF  + N  +  S     E FE YC ++ +TAAWGGQLEL A
Sbjct: 197 TLRNQTAKYIHSHFDDFLPFLTNPNTGDMYS----KEEFEKYCDDIANTAAWGGQLELRA 256

Query: 263 LTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKHAFGLGEHYNSV 312
           L+H L+  I +     P + +G+EY    ++     L Y +HA+GLGEHYNSV
Sbjct: 257 LSHILQTPIEVVQMDSPSIIVGEEYSGKPII-----LVYMRHAYGLGEHYNSV 290

[Close]

BLAST of Carg22159-RA vs. ExPASy Swiss-Prot
Match: Q8N6M0 (Deubiquitinase OTUD6B OS=Homo sapiens OX=9606 GN=OTUD6B PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.9e-35
Identity = 105/302 (34.77%), Postives = 168/302 (55.63%), Query Frame = 0


Query: 11  DKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQVEEEISQLSA 70
           + V  E  +  +++L RHRKE  +LQ K   +K A  +      K ++KQ+ E++++L  
Sbjct: 2   EAVLTEELDEEEQLLRRHRKEKKELQAKIQGMKNAVPK----NDKKRRKQLTEDVAKLEK 61

Query: 71  KLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKSTKRREKRAQQ 130
           ++++KH EEL  L  ++    + + +D++  AV   ++  ++   + SK+ KRREK+A  
Sbjct: 62  EMEQKHREELEQLKLTT----KENKIDSV--AVNISNLVLENQPPRISKAQKRREKKAAL 121

Query: 131 EAARDRRIQE-EQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRAVENQMDFLS 190
           E  R+ RI E E  N    R +E+E+L + L    L + +I  DGHC+Y+A+E+Q   L 
Sbjct: 122 EKEREERIAEAEIENLTGARHMESEKLAQILAARQLEIKQIPSDGHCMYKAIEDQ---LK 181

Query: 191 GGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENYCKEVESTAA 250
                     LR   A YM+ H  D++PF  + N      +    E F+ YC+++ +TAA
Sbjct: 182 EKDCALTVVALRSQTAEYMQSHVEDFLPFLTNPN----TGDMYTPEEFQKYCEDIVNTAA 241

Query: 251 WGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKHAFGLGEHYN 310
           WGGQLEL AL+H L+  I I     P + +G+EY    ++     L Y +HA+GLGEHYN
Sbjct: 242 WGGQLELRALSHILQTPIEIIQADSPPIIVGEEYSKKPLI-----LVYMRHAYGLGEHYN 281

Query: 311 SV 312
           SV
Sbjct: 302 SV 281

[Close]

BLAST of Carg22159-RA vs. ExPASy Swiss-Prot
Match: Q5M8L0 (Deubiquitinase OTUD6B OS=Xenopus tropicalis OX=8364 GN=otud6b PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 8.6e-35
Identity = 104/297 (35.02%), Postives = 157/297 (52.86%), Query Frame = 0


Query: 16  ENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQVEEEISQLSAKLKKK 75
           E +     ++ +HRKE   LQ K   +K +  +      K ++KQ+ E+I++L A L  +
Sbjct: 5   EGNSEESGLIKQHRKEKRDLQAKIQSMKNSVPK----NDKKRRKQLTEDIAKLEADLDVR 64

Query: 76  HNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKSTKRREKRAQQEAARD 135
           H EEL           + S + N V ++  +   A     + SK+ KRREK+A QE  RD
Sbjct: 65  HKEELEAFAQKQPEPTQVSGITNGVTSL-DLGNEAPVQQPRQSKAQKRREKKAAQEKERD 124

Query: 136 RRIQE-EQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRAVENQMDFLSGGSSP 195
            RI E E +N    R +E+++L + L    L + +I  DGHC+YRA+E+Q   L    + 
Sbjct: 125 ERIAEAEIANLSGARHLESQKLARILAERELQIRQIPSDGHCMYRAIEHQ---LRERGND 184

Query: 196 YNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENYCKEVESTAAWGGQL 255
                LR   A YM++H+ D++PF  + +      +    E F  YC ++ +T AWGGQL
Sbjct: 185 LTVANLRSQTADYMQNHAEDFLPFLTNSS----TGDMYTQEEFLKYCTDIVNTPAWGGQL 244

Query: 256 ELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKHAFGLGEHYNSV 312
           EL AL+H LK  I +       + +G+EY S      +I L Y +HA+GLGEHYNSV
Sbjct: 245 ELRALSHILKTAIEVIQAESSPIVIGEEYSS-----KAITLVYMRHAYGLGEHYNSV 284

[Close]

BLAST of Carg22159-RA vs. ExPASy TrEMBL
Match: A0A6J1KQW3 (OTU domain-containing protein 6B OS=Cucurbita maxima OX=3661 GN=LOC111497853 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.1e-165
Identity = 308/314 (98.09%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60
           MEDASSETITDKVSQENHETRDE+LSRHRKEISQLQNKEMELKKAAARGSKAEQK+KKKQ
Sbjct: 1   MEDASSETITDKVSQENHETRDEILSRHRKEISQLQNKEMELKKAAARGSKAEQKLKKKQ 60

Query: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120
           VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVS+TAQSDHSKPSKS
Sbjct: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSLTAQSDHSKPSKS 120

Query: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180
           TKRREKRAQQEAARDRRI+EEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA
Sbjct: 121 TKRREKRAQQEAARDRRIEEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180

Query: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240
           VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY
Sbjct: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240

Query: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300
           CKEVESTAAWGGQLELGALTHCL+KHI+IFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH
Sbjct: 241 CKEVESTAAWGGQLELGALTHCLRKHIIIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300

Query: 301 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVVPV
Sbjct: 301 AFGLGEHYNSVVPV 314

[Close]

BLAST of Carg22159-RA vs. ExPASy TrEMBL
Match: A0A6J1H6A0 (OTU domain-containing protein 6B isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460842 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.1e-165
Identity = 310/314 (98.73%), Postives = 311/314 (99.04%), Query Frame = 0


Query: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60
           MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ
Sbjct: 6   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 65

Query: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120
           VEEEISQLSA LKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVS TAQSDHSKPSKS
Sbjct: 66  VEEEISQLSANLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSRTAQSDHSKPSKS 125

Query: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180
           TKRREKRAQQEAARDRRI+EEQSN VSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA
Sbjct: 126 TKRREKRAQQEAARDRRIEEEQSNIVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 185

Query: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240
           VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY
Sbjct: 186 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 245

Query: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300
           CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH
Sbjct: 246 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 305

Query: 301 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVVPV
Sbjct: 306 AFGLGEHYNSVVPV 319

[Close]

BLAST of Carg22159-RA vs. ExPASy TrEMBL
Match: A0A6J1H5Y8 (OTU domain-containing protein 6B isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460842 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.1e-165
Identity = 310/314 (98.73%), Postives = 311/314 (99.04%), Query Frame = 0


Query: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60
           MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ
Sbjct: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60

Query: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120
           VEEEISQLSA LKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVS TAQSDHSKPSKS
Sbjct: 61  VEEEISQLSANLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSRTAQSDHSKPSKS 120

Query: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180
           TKRREKRAQQEAARDRRI+EEQSN VSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA
Sbjct: 121 TKRREKRAQQEAARDRRIEEEQSNIVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180

Query: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240
           VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY
Sbjct: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240

Query: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300
           CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH
Sbjct: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300

Query: 301 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVVPV
Sbjct: 301 AFGLGEHYNSVVPV 314

[Close]

BLAST of Carg22159-RA vs. ExPASy TrEMBL
Match: A0A6J1GQT5 (OTU domain-containing protein 6B-like OS=Cucurbita moschata OX=3662 GN=LOC111456222 PE=4 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.0e-151
Identity = 280/314 (89.17%), Postives = 302/314 (96.18%), Query Frame = 0


Query: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60
           +ED SSET++D V+QENHETRDEMLSRHRKEISQLQNKE+ELKKAAARGSKAEQKMKKKQ
Sbjct: 7   IEDTSSETVSDTVAQENHETRDEMLSRHRKEISQLQNKEIELKKAAARGSKAEQKMKKKQ 66

Query: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120
           VEEEISQLSAKLKKKH EELA LG+S SNGNE++N++NLVKA+AGVS TAQSDHSKPSKS
Sbjct: 67  VEEEISQLSAKLKKKHTEELASLGFSDSNGNEKNNIENLVKAIAGVSTTAQSDHSKPSKS 126

Query: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180
           T+RREKRAQQEA RDRRI+EEQSN VSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA
Sbjct: 127 TRRREKRAQQEAERDRRIEEEQSNIVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 186

Query: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240
           VE+Q+  LSGGSSPYNYQELR+MVAAYMRDHSTDYMPFFLS+NVVEGNS+ S++ERFENY
Sbjct: 187 VESQLALLSGGSSPYNYQELREMVAAYMRDHSTDYMPFFLSDNVVEGNSDVSMAERFENY 246

Query: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300
           CKE+ESTAAWGGQLELGALTHCLKKHIMIFSG FPDVEMGKEYKSDGVLNSS+RLSYHKH
Sbjct: 247 CKEIESTAAWGGQLELGALTHCLKKHIMIFSGCFPDVEMGKEYKSDGVLNSSVRLSYHKH 306

Query: 301 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVVPV
Sbjct: 307 AFGLGEHYNSVVPV 320

[Close]

BLAST of Carg22159-RA vs. ExPASy TrEMBL
Match: A0A6J1JQT7 (OTU domain-containing protein 6B-like OS=Cucurbita maxima OX=3661 GN=LOC111488073 PE=4 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 5.4e-149
Identity = 276/314 (87.90%), Postives = 298/314 (94.90%), Query Frame = 0


Query: 1   MEDASSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQ 60
           +ED SSET++D  +QENHETRDEMLSRH KEISQLQNKE+ELKKAAARGSKAEQKMKKKQ
Sbjct: 7   IEDTSSETVSDTAAQENHETRDEMLSRHGKEISQLQNKEIELKKAAARGSKAEQKMKKKQ 66

Query: 61  VEEEISQLSAKLKKKHNEELAFLGYSSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKS 120
           VEEEISQLSAKLKKKH EELA LG+S SNGNE++N++NLVKA+AGVS T QSDHSKPSKS
Sbjct: 67  VEEEISQLSAKLKKKHTEELASLGFSDSNGNEKNNIENLVKAIAGVSTTGQSDHSKPSKS 126

Query: 121 TKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRA 180
           T+RREKRAQQEA RDRRI+EEQSN VSDRM+ENEQLEKKLGPLGLTVNEIKPDGHCLYRA
Sbjct: 127 TRRREKRAQQEAERDRRIEEEQSNIVSDRMVENEQLEKKLGPLGLTVNEIKPDGHCLYRA 186

Query: 181 VENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNESLSERFENY 240
           VE+Q+  LSGGSSPYNYQELR+MVAAYMRDHSTDYMPFFLS+NVVEGNS+ S++ RFENY
Sbjct: 187 VESQLALLSGGSSPYNYQELREMVAAYMRDHSTDYMPFFLSDNVVEGNSDVSMAARFENY 246

Query: 241 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 300
           CKE+ESTAAWGGQLELGALTHCLKKHIMIFSG FPDVEMGKEYKSDGVLNSSIRLSYHKH
Sbjct: 247 CKEIESTAAWGGQLELGALTHCLKKHIMIFSGCFPDVEMGKEYKSDGVLNSSIRLSYHKH 306

Query: 301 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVVPV
Sbjct: 307 AFGLGEHYNSVVPV 320

[Close]

BLAST of Carg22159-RA vs. TAIR 10
Match: AT3G62940.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 397.5 bits (1020), Expect = 9.9e-111
Identity = 215/320 (67.19%), Postives = 261/320 (81.56%), Query Frame = 0


Query: 5   SSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQVEEE 64
           S ET  D   ++ HET +EML+RHR+EI QLQNKE E+KKAAA+GSKAEQK KKKQVE++
Sbjct: 12  SEETPMDASQEQQHETVEEMLARHRQEIKQLQNKETEMKKAAAKGSKAEQKAKKKQVEDD 71

Query: 65  ISQLSAKLKKKHNEELAFLGYSSSNGN---------ERSNLDNLVKAVAGVSMTAQSDHS 124
           IS+LS KLK K  +ELA  G+SSS+ N         ++ ++D LV+A+AGVS+TAQ +HS
Sbjct: 72  ISKLSTKLKDKQLKELASQGFSSSSSNNIAKDETTEKKGDIDTLVRAIAGVSVTAQQEHS 131

Query: 125 KPSKSTKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGH 184
           KPSKS KRREKRA++EA R++RI+EEQS+  SDRM+EN +LEKKL PLGLTV+EIKPDGH
Sbjct: 132 KPSKSVKRREKRAKEEADREQRIKEEQSHVKSDRMVENAKLEKKLKPLGLTVSEIKPDGH 191

Query: 185 CLYRAVENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNE-SLS 244
           CLYRAVENQ+   SGG+SPY YQ LR+M A+YMR+H TD++PFFLSE   EG+SN  S  
Sbjct: 192 CLYRAVENQLANRSGGASPYTYQNLREMAASYMREHKTDFLPFFLSE--TEGDSNSGSAE 251

Query: 245 ERFENYCKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIR 304
           ERFE YC+EVESTAAWG QLELGALTHCL+KHI ++SGSFPDVEMGKEY+S    +SS+ 
Sbjct: 252 ERFEKYCREVESTAAWGSQLELGALTHCLRKHIKVYSGSFPDVEMGKEYRSGD--DSSLM 311

Query: 305 LSYHKHAFGLGEHYNSVVPV 315
           LSYH+HAFGLGEHYNSVV V
Sbjct: 312 LSYHRHAFGLGEHYNSVVLV 327

[Close]

BLAST of Carg22159-RA vs. TAIR 10
Match: AT3G62940.3 (Cysteine proteinases superfamily protein )

HSP 1 Score: 397.5 bits (1020), Expect = 9.9e-111
Identity = 215/320 (67.19%), Postives = 261/320 (81.56%), Query Frame = 0


Query: 5   SSETITDKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQVEEE 64
           S ET  D   ++ HET +EML+RHR+EI QLQNKE E+KKAAA+GSKAEQK KKKQVE++
Sbjct: 12  SEETPMDASQEQQHETVEEMLARHRQEIKQLQNKETEMKKAAAKGSKAEQKAKKKQVEDD 71

Query: 65  ISQLSAKLKKKHNEELAFLGYSSSNGN---------ERSNLDNLVKAVAGVSMTAQSDHS 124
           IS+LS KLK K  +ELA  G+SSS+ N         ++ ++D LV+A+AGVS+TAQ +HS
Sbjct: 72  ISKLSTKLKDKQLKELASQGFSSSSSNNIAKDETTEKKGDIDTLVRAIAGVSVTAQQEHS 131

Query: 125 KPSKSTKRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGH 184
           KPSKS KRREKRA++EA R++RI+EEQS+  SDRM+EN +LEKKL PLGLTV+EIKPDGH
Sbjct: 132 KPSKSVKRREKRAKEEADREQRIKEEQSHVKSDRMVENAKLEKKLKPLGLTVSEIKPDGH 191

Query: 185 CLYRAVENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNE-SLS 244
           CLYRAVENQ+   SGG+SPY YQ LR+M A+YMR+H TD++PFFLSE   EG+SN  S  
Sbjct: 192 CLYRAVENQLANRSGGASPYTYQNLREMAASYMREHKTDFLPFFLSE--TEGDSNSGSAE 251

Query: 245 ERFENYCKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIR 304
           ERFE YC+EVESTAAWG QLELGALTHCL+KHI ++SGSFPDVEMGKEY+S    +SS+ 
Sbjct: 252 ERFEKYCREVESTAAWGSQLELGALTHCLRKHIKVYSGSFPDVEMGKEYRSGD--DSSLM 311

Query: 305 LSYHKHAFGLGEHYNSVVPV 315
           LSYH+HAFGLGEHYNSVV V
Sbjct: 312 LSYHRHAFGLGEHYNSVVLV 327

[Close]

BLAST of Carg22159-RA vs. TAIR 10
Match: AT3G62940.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 394.0 bits (1011), Expect = 1.1e-109
Identity = 212/314 (67.52%), Postives = 258/314 (82.17%), Query Frame = 0


Query: 11  DKVSQENHETRDEMLSRHRKEISQLQNKEMELKKAAARGSKAEQKMKKKQVEEEISQLSA 70
           D   ++ HET +EML+RHR+EI QLQNKE E+KKAAA+GSKAEQK KKKQVE++IS+LS 
Sbjct: 2   DASQEQQHETVEEMLARHRQEIKQLQNKETEMKKAAAKGSKAEQKAKKKQVEDDISKLST 61

Query: 71  KLKKKHNEELAFLGYSSSNGN---------ERSNLDNLVKAVAGVSMTAQSDHSKPSKST 130
           KLK K  +ELA  G+SSS+ N         ++ ++D LV+A+AGVS+TAQ +HSKPSKS 
Sbjct: 62  KLKDKQLKELASQGFSSSSSNNIAKDETTEKKGDIDTLVRAIAGVSVTAQQEHSKPSKSV 121

Query: 131 KRREKRAQQEAARDRRIQEEQSNTVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRAV 190
           KRREKRA++EA R++RI+EEQS+  SDRM+EN +LEKKL PLGLTV+EIKPDGHCLYRAV
Sbjct: 122 KRREKRAKEEADREQRIKEEQSHVKSDRMVENAKLEKKLKPLGLTVSEIKPDGHCLYRAV 181

Query: 191 ENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTDYMPFFLSENVVEGNSNE-SLSERFENY 250
           ENQ+   SGG+SPY YQ LR+M A+YMR+H TD++PFFLSE   EG+SN  S  ERFE Y
Sbjct: 182 ENQLANRSGGASPYTYQNLREMAASYMREHKTDFLPFFLSE--TEGDSNSGSAEERFEKY 241

Query: 251 CKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKH 310
           C+EVESTAAWG QLELGALTHCL+KHI ++SGSFPDVEMGKEY+S    +SS+ LSYH+H
Sbjct: 242 CREVESTAAWGSQLELGALTHCLRKHIKVYSGSFPDVEMGKEYRSGD--DSSLMLSYHRH 301

Query: 311 AFGLGEHYNSVVPV 315
           AFGLGEHYNSVV V
Sbjct: 302 AFGLGEHYNSVVLV 311

[Close]

BLAST of Carg22159-RA vs. TAIR 10
Match: AT5G67170.1 (SEC-C motif-containing protein / OTU-like cysteine protease family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-10
Identity = 45/157 (28.66%), Postives = 71/157 (45.22%), Query Frame = 0


Query: 155 QLEKKLGPLGLTVNEIKPDGHCLYRAVENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTD 214
           Q   +L  LGL + ++  DG+C +RA+ +Q   L G    +N  + R M+  Y+  +   
Sbjct: 27  QFRAQLDALGLKIIQVTADGNCFFRAIADQ---LEGNEDEHN--KYRNMIVLYIVKNREM 86

Query: 215 YMPFFLSENVVEGNSNESLSERFENYCKEVESTAAWGGQLELGALTHCLKKHIMIFSGSF 274
           + P F+ ++V            FE+YCK ++    W G +EL A +   + +I I     
Sbjct: 87  FEP-FIEDDV-----------PFEDYCKTMDDDGTWAGNMELQAASLVTRSNICIHRNMS 146

Query: 275 PDVEMGKEYKSDGVLNSSIRLSYHKHAFGLGEHYNSV 312
           P   +      +      I LSYH      GEHYNSV
Sbjct: 147 PRWYI---RNFEDTRTRMIHLSYHD-----GEHYNSV 158

[Close]

BLAST of Carg22159-RA vs. TAIR 10
Match: AT5G67170.2 (SEC-C motif-containing protein / OTU-like cysteine protease family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-10
Identity = 45/157 (28.66%), Postives = 71/157 (45.22%), Query Frame = 0


Query: 155 QLEKKLGPLGLTVNEIKPDGHCLYRAVENQMDFLSGGSSPYNYQELRQMVAAYMRDHSTD 214
           Q   +L  LGL + ++  DG+C +RA+ +Q   L G    +N  + R M+  Y+  +   
Sbjct: 26  QFRAQLDALGLKIIQVTADGNCFFRAIADQ---LEGNEDEHN--KYRNMIVLYIVKNREM 85

Query: 215 YMPFFLSENVVEGNSNESLSERFENYCKEVESTAAWGGQLELGALTHCLKKHIMIFSGSF 274
           + P F+ ++V            FE+YCK ++    W G +EL A +   + +I I     
Sbjct: 86  FEP-FIEDDV-----------PFEDYCKTMDDDGTWAGNMELQAASLVTRSNICIHRNMS 145

Query: 275 PDVEMGKEYKSDGVLNSSIRLSYHKHAFGLGEHYNSV 312
           P   +      +      I LSYH      GEHYNSV
Sbjct: 146 PRWYI---RNFEDTRTRMIHLSYHD-----GEHYNSV 157

The following BLAST results are available for this feature:

BLAST of Carg22159-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6593166.1	7.0e-167	100.00	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 5, partial [Cucurbita ar...	[more]
KAG7025564.1	7.0e-167	100.00	OTU domain-containing protein 6B [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023514920.1	1.7e-165	99.04	OTU domain-containing protein 6B isoform X4 [Cucurbita pepo subsp. pepo]	[more]
XP_023514919.1	1.7e-165	99.04	OTU domain-containing protein 6B isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_023004601.1	1.5e-164	98.09	OTU domain-containing protein 6B [Cucurbita maxima] >XP_023004602.1 OTU domain-c...	[more]

BLAST of Carg22159-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LYC7	1.4e-109	67.19	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 5 OS=Arabidopsis thalian...	[more]
Q7ZV00	8.3e-38	38.54	Deubiquitinase OTUD6B OS=Danio rerio OX=7955 GN=otud6b PE=2 SV=1	[more]
Q5ZIP6	1.9e-37	36.18	Deubiquitinase OTUD6B OS=Gallus gallus OX=9031 GN=OTUD6B PE=2 SV=1	[more]
Q8N6M0	3.9e-35	34.77	Deubiquitinase OTUD6B OS=Homo sapiens OX=9606 GN=OTUD6B PE=1 SV=1	[more]
Q5M8L0	8.6e-35	35.02	Deubiquitinase OTUD6B OS=Xenopus tropicalis OX=8364 GN=otud6b PE=2 SV=1	[more]

BLAST of Carg22159-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KQW3	7.1e-165	98.09	OTU domain-containing protein 6B OS=Cucurbita maxima OX=3661 GN=LOC111497853 PE=...	[more]
A0A6J1H6A0	7.1e-165	98.73	OTU domain-containing protein 6B isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1H5Y8	7.1e-165	98.73	OTU domain-containing protein 6B isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1GQT5	2.0e-151	89.17	OTU domain-containing protein 6B-like OS=Cucurbita moschata OX=3662 GN=LOC111456...	[more]
A0A6J1JQT7	5.4e-149	87.90	OTU domain-containing protein 6B-like OS=Cucurbita maxima OX=3661 GN=LOC11148807...	[more]

BLAST of Carg22159-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62940.2	9.9e-111	67.19	Cysteine proteinases superfamily protein	[more]
AT3G62940.3	9.9e-111	67.19	Cysteine proteinases superfamily protein	[more]
AT3G62940.1	1.1e-109	67.52	Cysteine proteinases superfamily protein	[more]
AT5G67170.1	1.5e-10	28.66	SEC-C motif-containing protein / OTU-like cysteine protease family protein	[more]
AT5G67170.2	1.5e-10	28.66	SEC-C motif-containing protein / OTU-like cysteine protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 25..78
None	No IPR available	GENE3D	3.90.70.80		coord: 151..314 e-value: 2.9E-33 score: 116.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 116..143
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 43..65
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 107..145
None	No IPR available	PANTHER	PTHR12419	OTU DOMAIN CONTAINING PROTEIN	coord: 19..313
None	No IPR available	PANTHER	PTHR12419:SF10	DEUBIQUITINASE OTUD6B	coord: 19..313
IPR003323	OTU domain	PFAM	PF02338	OTU	coord: 171..308 e-value: 1.6E-32 score: 112.6
IPR003323	OTU domain	PROSITE	PS50802	OTU	coord: 165..314 score: 27.83872
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 152..313

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg22159	Carg22159	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22159-RA:3	Carg22159-RA:3	exon
Carg22159-RA:2	Carg22159-RA:2	exon
Carg22159-RA:1	Carg22159-RA:1	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22159-RA:five_prime_utr	Carg22159-RA:five_prime_utr	five_prime_UTR
Carg22159-RA:five_prime_utr	Carg22159-RA:five_prime_utr_2	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22159-RA:cds	Carg22159-RA:cds	CDS
Carg22159-RA:cds	Carg22159-RA:cds_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg22159-RA	Carg22159-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016579	protein deubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004843	thiol-dependent deubiquitinase