Carg22144-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22144-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Membrane-associated kinase regulator 5
Location	Carg_Chr18: 11528282 .. 11529145 (-)
Sequence length	864
RNA-Seq Expression	Carg22144-RA
Synteny	Carg22144-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCTCTGGACTTCGTCAAGCTTCATAATCTCTACGCCCATTTCTCCTCCATTGTTGAAACTGCATATCCTCTCATTCGCTCTCGTTTCTATGGCGACGATCAGGATGAAGATTCATTCTTCGATTTGGATTTCATCTCTCCTCGTTCTTCAGGATCTGGAGAATCTCCTGACCAAAGGAACCTCTCTCTCTCGGGCGAAACCGATCTCTGTTTCTCTCAACGGAATCTTCTTCTCTCCCAATCTGATCTCATCTCGAAACGAAAAATCCTTCCGATTGAACTCAGTTCGAAGCCAGAGTCTCCGGTTCCAGTTTCGATTCCTAAATCGGCACCTAGACTCTCGATTTCCTTGTTCAAGAAGCCGAAATTAATGGCGAAACAAAGAACGGACGAAACAGATTCGCCGTTGTCTCTCAAATTACAGAGCACAGAAAGCAAACGATTCACTTTCAAACTGAACAGAGTTAACAGTTCCAGAAACAGTAATCGAAATGACGCATCTGAGCATCAATCCAAGAGATTCTGCGGAGAAGGAATGCAGAAGTATCTGAAATTAATCAAGCCTCTGTATGTGAAAGTTTCAAGAAAACCTAACTACAGTTCGTCCGCGGCATCATCTCCGGTGACTACGACGGCTACATCGTTCGATGAAAAGCAGAGGAATATTTCGATAGGCATCCGAGTGGTTTGCAGGCGTCTAGGGAAGAGTAAATCGTCATCTTCAGCGACAGGAATGGCTGTATCGCCGACGAATCGGAGAGACGATTCGCTTTTACAGCAAGACGACGGGATTGAGAGCGCCATTTTGCATTGCAAAAGGTCATTCAGCGCTACCCGAGGCAAGATCCAAAAACAATAA

mRNA sequence

ATGGAAGCTCTGGACTTCGTCAAGCTTCATAATCTCTACGCCCATTTCTCCTCCATTGTTGAAACTGCATATCCTCTCATTCGCTCTCGTTTCTATGGCGACGATCAGGATGAAGATTCATTCTTCGATTTGGATTTCATCTCTCCTCGTTCTTCAGGATCTGGAGAATCTCCTGACCAAAGGAACCTCTCTCTCTCGGGCGAAACCGATCTCTGTTTCTCTCAACGGAATCTTCTTCTCTCCCAATCTGATCTCATCTCGAAACGAAAAATCCTTCCGATTGAACTCAGTTCGAAGCCAGAGTCTCCGGTTCCAGTTTCGATTCCTAAATCGGCACCTAGACTCTCGATTTCCTTGTTCAAGAAGCCGAAATTAATGGCGAAACAAAGAACGGACGAAACAGATTCGCCGTTGTCTCTCAAATTACAGAGCACAGAAAGCAAACGATTCACTTTCAAACTGAACAGAGTTAACAGTTCCAGAAACAGTAATCGAAATGACGCATCTGAGCATCAATCCAAGAGATTCTGCGGAGAAGGAATGCAGAAGTATCTGAAATTAATCAAGCCTCTGTATGTGAAAGTTTCAAGAAAACCTAACTACAGTTCGTCCGCGGCATCATCTCCGGTGACTACGACGGCTACATCGTTCGATGAAAAGCAGAGGAATATTTCGATAGGCATCCGAGTGGTTTGCAGGCGTCTAGGGAAGAGTAAATCGTCATCTTCAGCGACAGGAATGGCTGTATCGCCGACGAATCGGAGAGACGATTCGCTTTTACAGCAAGACGACGGGATTGAGAGCGCCATTTTGCATTGCAAAAGGTCATTCAGCGCTACCCGAGGCAAGATCCAAAAACAATAA

Coding sequence (CDS)

ATGGAAGCTCTGGACTTCGTCAAGCTTCATAATCTCTACGCCCATTTCTCCTCCATTGTTGAAACTGCATATCCTCTCATTCGCTCTCGTTTCTATGGCGACGATCAGGATGAAGATTCATTCTTCGATTTGGATTTCATCTCTCCTCGTTCTTCAGGATCTGGAGAATCTCCTGACCAAAGGAACCTCTCTCTCTCGGGCGAAACCGATCTCTGTTTCTCTCAACGGAATCTTCTTCTCTCCCAATCTGATCTCATCTCGAAACGAAAAATCCTTCCGATTGAACTCAGTTCGAAGCCAGAGTCTCCGGTTCCAGTTTCGATTCCTAAATCGGCACCTAGACTCTCGATTTCCTTGTTCAAGAAGCCGAAATTAATGGCGAAACAAAGAACGGACGAAACAGATTCGCCGTTGTCTCTCAAATTACAGAGCACAGAAAGCAAACGATTCACTTTCAAACTGAACAGAGTTAACAGTTCCAGAAACAGTAATCGAAATGACGCATCTGAGCATCAATCCAAGAGATTCTGCGGAGAAGGAATGCAGAAGTATCTGAAATTAATCAAGCCTCTGTATGTGAAAGTTTCAAGAAAACCTAACTACAGTTCGTCCGCGGCATCATCTCCGGTGACTACGACGGCTACATCGTTCGATGAAAAGCAGAGGAATATTTCGATAGGCATCCGAGTGGTTTGCAGGCGTCTAGGGAAGAGTAAATCGTCATCTTCAGCGACAGGAATGGCTGTATCGCCGACGAATCGGAGAGACGATTCGCTTTTACAGCAAGACGACGGGATTGAGAGCGCCATTTTGCATTGCAAAAGGTCATTCAGCGCTACCCGAGGCAAGATCCAAAAACAATAA

Protein sequence

MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQRNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLFKKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEGMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQKQ

Homology

[Close]

BLAST of Carg22144-RA vs. NCBI nr
Match: KAG7013275.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 542.0 bits (1395), Expect = 3.2e-150
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60
           MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60

Query: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120
           RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF
Sbjct: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180
           KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG
Sbjct: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180

Query: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240
           MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS
Sbjct: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240

Query: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQKQ 288
           SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQKQ
Sbjct: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKIQKQ 287

[Close]

BLAST of Carg22144-RA vs. NCBI nr
Match: KAG6574213.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 528.1 bits (1359), Expect = 4.8e-146
Identity = 280/284 (98.59%), Postives = 281/284 (98.94%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60
           MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60

Query: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120
           RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF
Sbjct: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180
           KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASE QSKRFCGEG
Sbjct: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASERQSKRFCGEG 180

Query: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240
           MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS
Sbjct: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240

Query: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKI 285
           SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR  +
Sbjct: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRDDV 284

[Close]

BLAST of Carg22144-RA vs. NCBI nr
Match: XP_022945795.1 (probable membrane-associated kinase regulator 5 [Cucurbita moschata])

HSP 1 Score: 526.2 bits (1354), Expect = 1.8e-145
Identity = 278/284 (97.89%), Postives = 280/284 (98.59%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60
           MEALDFVK HNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ
Sbjct: 1   MEALDFVKFHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60

Query: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120
           RNLSLSGE +LCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF
Sbjct: 61  RNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180
           KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG
Sbjct: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180

Query: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240
           MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS
Sbjct: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240

Query: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKI 285
           SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR  +
Sbjct: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRDDV 284

[Close]

BLAST of Carg22144-RA vs. NCBI nr
Match: XP_023541341.1 (probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 507.3 bits (1305), Expect = 8.7e-140
Identity = 271/284 (95.42%), Postives = 274/284 (96.48%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60
           MEALDFVKLHNLYAHFSSIVETAYPLIRSR YGDDQDEDSFFDLDFISP SSGSGESPDQ
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRLYGDDQDEDSFFDLDFISPPSSGSGESPDQ 60

Query: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120
            NLSLSGE +LCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF
Sbjct: 61  SNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180
           KKPKLMAKQRT+ETDS  SLKLQSTESKRFTFKLNRVNSSRNSNRNDASE QSKRFCGE 
Sbjct: 121 KKPKLMAKQRTEETDSLSSLKLQSTESKRFTFKLNRVNSSRNSNRNDASERQSKRFCGEA 180

Query: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240
           MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS
Sbjct: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240

Query: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKI 285
           SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR  +
Sbjct: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRDDV 284

[Close]

BLAST of Carg22144-RA vs. NCBI nr
Match: XP_022968326.1 (probable membrane-associated kinase regulator 5 [Cucurbita maxima])

HSP 1 Score: 504.2 bits (1297), Expect = 7.4e-139
Identity = 268/284 (94.37%), Postives = 274/284 (96.48%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60
           MEALDFVKLHNLYA+FSSIVETAYPLIRSR YGDDQDEDSFFDLDFISP SSGSGESPD+
Sbjct: 1   MEALDFVKLHNLYAYFSSIVETAYPLIRSRIYGDDQDEDSFFDLDFISPPSSGSGESPDR 60

Query: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120
           RNLSLSGE +LCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSA RLSISLF
Sbjct: 61  RNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSASRLSISLF 120

Query: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180
           KKPKLMAKQRTDETD+P SLKLQSTESKRFTFKLNRVNSSRNSNRNDASE QSKRFCGEG
Sbjct: 121 KKPKLMAKQRTDETDTPSSLKLQSTESKRFTFKLNRVNSSRNSNRNDASERQSKRFCGEG 180

Query: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240
           M+KYLKLIKPLYVKVSRKPNYSSS  SSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS
Sbjct: 181 MRKYLKLIKPLYVKVSRKPNYSSSPVSSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240

Query: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKI 285
           SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR  +
Sbjct: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRDDV 284

[Close]

BLAST of Carg22144-RA vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 6.7e-18
Identity = 98/294 (33.33%), Postives = 147/294 (50.00%), Query Frame = 0


Query: 1   MEALDFVKL---HNLYAHFSSIVETAYPLIRSRFYGDDQD------EDSFFDLDFISPRS 60
           MEAL F+K    +N        +  +   + S    +D D      EDSFF+L      S
Sbjct: 1   MEALTFMKFWLTNNTTIKPRREIRISESAVDSTTGSEDPDLDLCEGEDSFFELKI----S 60

Query: 61  SGSGESPDQRNLSLSGETDLCFSQRNLLLSQSDLISKRKILP-IELSSKPESPVPVSIPK 120
               ++P ++    +  T   +S  N          K K+LP +++SSKP+   P ++ K
Sbjct: 61  LSDFKTPKEKQRLETTTTTTTYSVSN----------KSKVLPFVDISSKPQQS-PTTLLK 120

Query: 121 SAPRLSISLFKK-PKLMAKQRTDETDSPLSLKLQ-STESKRFTFKLNRVNSSRNSNRNDA 180
           S  +     FKK  K    ++  E ++  SL ++   + +  T    +  S   S + D 
Sbjct: 121 SGQKFRAFSFKKSEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSFRKTASIARSQQTD- 180

Query: 181 SEHQSKRFCGEGMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQR-NISIG 240
                  F     +++  LIKPLY K ++K   SSS  +SP ++ AT   EKQR NI  G
Sbjct: 181 ----DTMFDDSVSKRFFSLIKPLYTKSTKK--QSSSTITSPTSSPATR--EKQRSNIPSG 240

Query: 241 IRVVCRRLGKSKSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR 282
           +R V R+LGKS+S+S+A G  +SP NR D+SL  Q DGI+SAILHCK+SF  +R
Sbjct: 241 MRSVRRQLGKSRSASAAIG-GMSPANRIDESLQVQQDGIQSAILHCKKSFHGSR 269

[Close]

BLAST of Carg22144-RA vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-16
Identity = 80/226 (35.40%), Postives = 113/226 (50.00%), Query Frame = 0


Query: 83  SDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLFKKPKLMAKQRTDETDSPLSLKL 142
           S+ + K+   P  LS  PES       KS   +++ + + P +    R + + +  S   
Sbjct: 185 SEDLDKQTHPPPPLSLSPESQ-----QKSTVTVNLKVEEVPIISLFSRENSSKNSSSSSS 244

Query: 143 QSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEGMQKYLKLIKPLYVKVSRK---- 202
            S+ S          +S +  N N++     KRF    MQKYLK +KPLY++VSR+    
Sbjct: 245 SSSSSS---------SSLKKQNGNESVVSDEKRFV--MMQKYLKKVKPLYIRVSRRYGEK 304

Query: 203 ---------PNYSSSA---ASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKSSSS--- 262
                    P+ + S    A SPV T   +      NI  G++VV + LGKS+SSSS   
Sbjct: 305 LRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTAT 364

Query: 263 ------ATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGK 284
                 AT    S + RRDDSLLQQ D I+SAILHCKRSF+++R K
Sbjct: 365 TPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

[Close]

BLAST of Carg22144-RA vs. ExPASy TrEMBL
Match: A0A6J1G1Z9 (probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN=LOC111449936 PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 8.8e-146
Identity = 278/284 (97.89%), Postives = 280/284 (98.59%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60
           MEALDFVK HNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ
Sbjct: 1   MEALDFVKFHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60

Query: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120
           RNLSLSGE +LCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF
Sbjct: 61  RNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120

Query: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180
           KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG
Sbjct: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180

Query: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240
           MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS
Sbjct: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240

Query: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKI 285
           SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR  +
Sbjct: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRDDV 284

[Close]

BLAST of Carg22144-RA vs. ExPASy TrEMBL
Match: A0A6J1HUJ8 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111467591 PE=4 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.6e-139
Identity = 268/284 (94.37%), Postives = 274/284 (96.48%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60
           MEALDFVKLHNLYA+FSSIVETAYPLIRSR YGDDQDEDSFFDLDFISP SSGSGESPD+
Sbjct: 1   MEALDFVKLHNLYAYFSSIVETAYPLIRSRIYGDDQDEDSFFDLDFISPPSSGSGESPDR 60

Query: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120
           RNLSLSGE +LCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSA RLSISLF
Sbjct: 61  RNLSLSGENELCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSASRLSISLF 120

Query: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180
           KKPKLMAKQRTDETD+P SLKLQSTESKRFTFKLNRVNSSRNSNRNDASE QSKRFCGEG
Sbjct: 121 KKPKLMAKQRTDETDTPSSLKLQSTESKRFTFKLNRVNSSRNSNRNDASERQSKRFCGEG 180

Query: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240
           M+KYLKLIKPLYVKVSRKPNYSSS  SSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS
Sbjct: 181 MRKYLKLIKPLYVKVSRKPNYSSSPVSSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240

Query: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKI 285
           SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR  +
Sbjct: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRDDV 284

[Close]

BLAST of Carg22144-RA vs. ExPASy TrEMBL
Match: A0A6J1J4Q0 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111482618 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.5e-116
Identity = 234/284 (82.39%), Postives = 255/284 (89.79%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60
           MEALDFVKLHNLYAHFSSIVETAY L RS F+GD+ DEDSFFDLDF+S  +S +G+SPD+
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYNLNRSHFHGDNHDEDSFFDLDFVSLPTSETGKSPDE 60

Query: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120
           +NL+ S E +LCF+QRNLLLSQSDLISKRKILPIELSSKPESPV VS+ KSAPRLSISLF
Sbjct: 61  KNLTRSSENELCFTQRNLLLSQSDLISKRKILPIELSSKPESPVSVSVLKSAPRLSISLF 120

Query: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180
           KKPKLMAKQ+TDET+ P SLKLQSTESKRF FKLNR+NSSRN+NR DASE QSKRF GEG
Sbjct: 121 KKPKLMAKQKTDETE-PSSLKLQSTESKRFAFKLNRLNSSRNNNRIDASERQSKRFSGEG 180

Query: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240
           +QKYLKLIKPLYVKVSRK NY SS  SSPV    TS DEKQRNI+IGIRVVCRRLG+SKS
Sbjct: 181 IQKYLKLIKPLYVKVSRKQNYRSSPVSSPV----TSIDEKQRNIAIGIRVVCRRLGRSKS 240

Query: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKI 285
           SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSA+R  +
Sbjct: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSASRDDV 279

[Close]

BLAST of Carg22144-RA vs. ExPASy TrEMBL
Match: A0A6J1CGZ9 (probable membrane-associated kinase regulator 5 OS=Momordica charantia OX=3673 GN=LOC111011435 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.5e-116
Identity = 232/284 (81.69%), Postives = 250/284 (88.03%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFISPRSSGSGESPDQ 60
           MEALDFVKLHNLYAHFSSIVETAYP+IRS FYGDDQDEDSFFDLDFISP  S + +S D 
Sbjct: 1   MEALDFVKLHNLYAHFSSIVETAYPMIRSHFYGDDQDEDSFFDLDFISPPPSETSKSSDS 60

Query: 61  RNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLF 120
           +NL+ SG+  LCF Q  LLLS SDLISKRKILPI+LSSKPESPVPV + KSAPRLSISLF
Sbjct: 61  KNLTRSGQNGLCFEQPGLLLSHSDLISKRKILPIDLSSKPESPVPVPLLKSAPRLSISLF 120

Query: 121 KKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEG 180
           KKPKLMAKQ+ DET+SP SLKLQ+TESKRF FKL+RV+SSRN+NR DASE QSKRF GEG
Sbjct: 121 KKPKLMAKQKMDETESPSSLKLQNTESKRFAFKLSRVSSSRNNNRMDASERQSKRFSGEG 180

Query: 181 MQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKS 240
           MQKYLKLIKPLYVKVSR+ NY +S  SSP    A+S DEKQRNISIGIRVVCRRLGKSKS
Sbjct: 181 MQKYLKLIKPLYVKVSRRHNYRASPVSSP--AAASSMDEKQRNISIGIRVVCRRLGKSKS 240

Query: 241 SSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGKI 285
           SSSATGMAVSPTNRRDDSLLQQ DGIESAILHCKRSFSATR  +
Sbjct: 241 SSSATGMAVSPTNRRDDSLLQQHDGIESAILHCKRSFSATRDDV 282

[Close]

BLAST of Carg22144-RA vs. ExPASy TrEMBL
Match: A0A0A0L6R5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184590 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 3.6e-115
Identity = 239/283 (84.45%), Postives = 254/283 (89.75%), Query Frame = 0


Query: 1   MEALDFVKLHNLYAHFSSIVETAYPLIRSRFYGDDQDEDSFFDLDFIS-PRSSGSGESPD 60
           MEALDFVKLHNLY+HFSSIVETAYP IRS FYGDDQDEDSFFDLDFIS P SS +G+SPD
Sbjct: 1   MEALDFVKLHNLYSHFSSIVETAYPFIRSHFYGDDQDEDSFFDLDFISLPPSSETGKSPD 60

Query: 61  QRNLSLSGETDLCFSQRNLLLSQSDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISL 120
           ++NL+ S + +L FSQRNLLLS SDLISKRKILPIELSSKPESPV VSI KSAPRLSISL
Sbjct: 61  EKNLTHSSQNELGFSQRNLLLSHSDLISKRKILPIELSSKPESPVSVSILKSAPRLSISL 120

Query: 121 FKKPKLMAKQRTDETDSPLSLKLQSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGE 180
           FKKPKLMAKQ+ DETDSP S KLQSTESKRFTFKLNRVNSSRN+NR DA+E QSKR   E
Sbjct: 121 FKKPKLMAKQKIDETDSP-SHKLQSTESKRFTFKLNRVNSSRNNNRIDATERQSKR---E 180

Query: 181 GMQKYLKLIKPLYVKVSRKPNYSSSAASSP-VTTTATSFDEKQRNISIGIRVVCRRLGKS 240
           GMQKYLKLIKPLYVKVSRK N+ SS  SSP  TTTA S DEKQRN +IGIRVVC+RLGKS
Sbjct: 181 GMQKYLKLIKPLYVKVSRKQNHRSSPVSSPSTTTTAPSIDEKQRNSAIGIRVVCKRLGKS 240

Query: 241 KSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR 282
           KSSSS+TGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR
Sbjct: 241 KSSSSSTGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR 279

[Close]

BLAST of Carg22144-RA vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 92.8 bits (229), Expect = 4.8e-19
Identity = 98/294 (33.33%), Postives = 147/294 (50.00%), Query Frame = 0


Query: 1   MEALDFVKL---HNLYAHFSSIVETAYPLIRSRFYGDDQD------EDSFFDLDFISPRS 60
           MEAL F+K    +N        +  +   + S    +D D      EDSFF+L      S
Sbjct: 1   MEALTFMKFWLTNNTTIKPRREIRISESAVDSTTGSEDPDLDLCEGEDSFFELKI----S 60

Query: 61  SGSGESPDQRNLSLSGETDLCFSQRNLLLSQSDLISKRKILP-IELSSKPESPVPVSIPK 120
               ++P ++    +  T   +S  N          K K+LP +++SSKP+   P ++ K
Sbjct: 61  LSDFKTPKEKQRLETTTTTTTYSVSN----------KSKVLPFVDISSKPQQS-PTTLLK 120

Query: 121 SAPRLSISLFKK-PKLMAKQRTDETDSPLSLKLQ-STESKRFTFKLNRVNSSRNSNRNDA 180
           S  +     FKK  K    ++  E ++  SL ++   + +  T    +  S   S + D 
Sbjct: 121 SGQKFRAFSFKKSEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSFRKTASIARSQQTD- 180

Query: 181 SEHQSKRFCGEGMQKYLKLIKPLYVKVSRKPNYSSSAASSPVTTTATSFDEKQR-NISIG 240
                  F     +++  LIKPLY K ++K   SSS  +SP ++ AT   EKQR NI  G
Sbjct: 181 ----DTMFDDSVSKRFFSLIKPLYTKSTKK--QSSSTITSPTSSPATR--EKQRSNIPSG 240

Query: 241 IRVVCRRLGKSKSSSSATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATR 282
           +R V R+LGKS+S+S+A G  +SP NR D+SL  Q DGI+SAILHCK+SF  +R
Sbjct: 241 MRSVRRQLGKSRSASAAIG-GMSPANRIDESLQVQQDGIQSAILHCKKSFHGSR 269

[Close]

BLAST of Carg22144-RA vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 88.6 bits (218), Expect = 9.0e-18
Identity = 80/226 (35.40%), Postives = 113/226 (50.00%), Query Frame = 0


Query: 83  SDLISKRKILPIELSSKPESPVPVSIPKSAPRLSISLFKKPKLMAKQRTDETDSPLSLKL 142
           S+ + K+   P  LS  PES       KS   +++ + + P +    R + + +  S   
Sbjct: 185 SEDLDKQTHPPPPLSLSPESQ-----QKSTVTVNLKVEEVPIISLFSRENSSKNSSSSSS 244

Query: 143 QSTESKRFTFKLNRVNSSRNSNRNDASEHQSKRFCGEGMQKYLKLIKPLYVKVSRK---- 202
            S+ S          +S +  N N++     KRF    MQKYLK +KPLY++VSR+    
Sbjct: 245 SSSSSS---------SSLKKQNGNESVVSDEKRFV--MMQKYLKKVKPLYIRVSRRYGEK 304

Query: 203 ---------PNYSSSA---ASSPVTTTATSFDEKQRNISIGIRVVCRRLGKSKSSSS--- 262
                    P+ + S    A SPV T   +      NI  G++VV + LGKS+SSSS   
Sbjct: 305 LRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTAT 364

Query: 263 ------ATGMAVSPTNRRDDSLLQQDDGIESAILHCKRSFSATRGK 284
                 AT    S + RRDDSLLQQ D I+SAILHCKRSF+++R K
Sbjct: 365 TPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

The following BLAST results are available for this feature:

BLAST of Carg22144-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013275.1	3.2e-150	100.00	putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma...	[more]
KAG6574213.1	4.8e-146	98.59	putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma...	[more]
XP_022945795.1	1.8e-145	97.89	probable membrane-associated kinase regulator 5 [Cucurbita moschata]	[more]
XP_023541341.1	8.7e-140	95.42	probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo]	[more]
XP_022968326.1	7.4e-139	94.37	probable membrane-associated kinase regulator 5 [Cucurbita maxima]	[more]

BLAST of Carg22144-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLX4	6.7e-18	33.33	Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 ...	[more]
Q9SH58	1.3e-16	35.40	Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Carg22144-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G1Z9	8.8e-146	97.89	probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1HUJ8	3.6e-139	94.37	probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1J4Q0	1.5e-116	82.39	probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1CGZ9	1.5e-116	81.69	probable membrane-associated kinase regulator 5 OS=Momordica charantia OX=3673 G...	[more]
A0A0A0L6R5	3.6e-115	84.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184590 PE=4 SV=1	[more]

BLAST of Carg22144-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52870.1	4.8e-19	33.33	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G64080.1	9.0e-18	35.40	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 156..175
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 239..256
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 239..259
None	No IPR available	PANTHER	PTHR33929:SF4	MEMBRANE-ASSOCIATED KINASE REGULATOR 5-RELATED	coord: 1..280
IPR039619	Membrane-associated kinase regulator 2/5	PANTHER	PTHR33929	MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATED	coord: 1..280

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg22144	Carg22144	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22144-RA:1	Carg22144-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22144-RA:cds	Carg22144-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg22144-RA	Carg22144-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0016301	kinase activity