Carg21937-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21937-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type E3 ubiquitin transferase
Location	Carg_Chr11: 3254249 .. 3254767 (-)
Sequence length	519
RNA-Seq Expression	Carg21937-RA
Synteny	Carg21937-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGATTCTGCTTCAACCTTTATTGCTCAGCCAACGTCTCATCCTGCAAAATCCAGCCTACCCATGTTGTATTATGGCCTTGTGGTCATCGGAACGGCGACGATTGTGTTGGTTCTTTACAATCTCATCATTATCAAGTGGTGCTCCGACGACAGCCACGGGCAAGCTTCACCGGCACTGGCTGGAAGTTATGCAGAGATGGTGAATTCAAGCCGTAGCTTCCAGAGTTCTAATAGGAATTTGTTGTCGAGCTTCAAATTCAAGAAGGGGTCGATGGCGACGGCGGCGGCGACTGCGACGATCGAGGAAGAAGGGATTGGGAATGAGTGTGCAGTTTGTTTATCGGCGTTTGAGGATGGGGAAGAGGTGAAGAAACTGCCAAGATGCACGCATATGTTTCACGCCCCTTGTATAGACATGTGGCTTTACTCTCACTCCGATTGCCCCCTCTGCCGTGCACCGGTGATGGCACCGGCGCTGTGCCAACGCCACACGGCGGAGGCTGCGGCGGAGTAA

mRNA sequence

ATGGGTGATTCTGCTTCAACCTTTATTGCTCAGCCAACGTCTCATCCTGCAAAATCCAGCCTACCCATGTTGTATTATGGCCTTGTGGTCATCGGAACGGCGACGATTGTGTTGGTTCTTTACAATCTCATCATTATCAAGTGGTGCTCCGACGACAGCCACGGGCAAGCTTCACCGGCACTGGCTGGAAGTTATGCAGAGATGGTGAATTCAAGCCGTAGCTTCCAGAGTTCTAATAGGAATTTGTTGTCGAGCTTCAAATTCAAGAAGGGGTCGATGGCGACGGCGGCGGCGACTGCGACGATCGAGGAAGAAGGGATTGGGAATGAGTGTGCAGTTTGTTTATCGGCGTTTGAGGATGGGGAAGAGGTGAAGAAACTGCCAAGATGCACGCATATGTTTCACGCCCCTTGTATAGACATGTGGCTTTACTCTCACTCCGATTGCCCCCTCTGCCGTGCACCGGTGATGGCACCGGCGCTGTGCCAACGCCACACGGCGGAGGCTGCGGCGGAGTAA

Coding sequence (CDS)

ATGGGTGATTCTGCTTCAACCTTTATTGCTCAGCCAACGTCTCATCCTGCAAAATCCAGCCTACCCATGTTGTATTATGGCCTTGTGGTCATCGGAACGGCGACGATTGTGTTGGTTCTTTACAATCTCATCATTATCAAGTGGTGCTCCGACGACAGCCACGGGCAAGCTTCACCGGCACTGGCTGGAAGTTATGCAGAGATGGTGAATTCAAGCCGTAGCTTCCAGAGTTCTAATAGGAATTTGTTGTCGAGCTTCAAATTCAAGAAGGGGTCGATGGCGACGGCGGCGGCGACTGCGACGATCGAGGAAGAAGGGATTGGGAATGAGTGTGCAGTTTGTTTATCGGCGTTTGAGGATGGGGAAGAGGTGAAGAAACTGCCAAGATGCACGCATATGTTTCACGCCCCTTGTATAGACATGTGGCTTTACTCTCACTCCGATTGCCCCCTCTGCCGTGCACCGGTGATGGCACCGGCGCTGTGCCAACGCCACACGGCGGAGGCTGCGGCGGAGTAA

Protein sequence

MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPALAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAEAAAE

Homology

[Close]

BLAST of Carg21937-RA vs. NCBI nr
Match: KAG6588064.1 (RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021960.1 RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 344.0 bits (881), Expect = 7.6e-91
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60
           MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120
           LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120

Query: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAEAAAE 173
           GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAEAAAE
Sbjct: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAEAAAE 172

[Close]

BLAST of Carg21937-RA vs. NCBI nr
Match: XP_022927302.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita moschata])

HSP 1 Score: 335.5 bits (859), Expect = 2.7e-88
Identity = 167/168 (99.40%), Postives = 167/168 (99.40%), Query Frame = 0


Query: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60
           MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQAS A
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASTA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120
           LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120

Query: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAE 169
           GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAE
Sbjct: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAE 168

[Close]

BLAST of Carg21937-RA vs. NCBI nr
Match: XP_022968369.1 (RING-H2 finger protein ATL52-like [Cucurbita maxima])

HSP 1 Score: 327.8 bits (839), Expect = 5.7e-86
Identity = 166/172 (96.51%), Postives = 167/172 (97.09%), Query Frame = 0


Query: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60
           MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSD+SHGQASPA
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDNSHGQASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120
           LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGS  TA ATATIEEEGIGNECAVCLSAFED
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGS--TAKATATIEEEGIGNECAVCLSAFED 120

Query: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAEAAAE 173
           GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAP MAPALCQRHTAE AAE
Sbjct: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPAMAPALCQRHTAEDAAE 170

[Close]

BLAST of Carg21937-RA vs. NCBI nr
Match: KAG6590024.1 (RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023686.1 RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 267.3 bits (682), Expect = 9.1e-68
Identity = 135/167 (80.84%), Postives = 142/167 (85.03%), Query Frame = 0


Query: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60
           M DSAS + AQPTSHPAKSSLPMLYYGLVVIGTA IVLVLYN I+I WCSD+  G+ASPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120
           LAGSYAEMVNSSRS QSSNRNLL SFKFKKGS   AAATA  EEEG GNEC VCLSAFE+
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGS--AAAATAAAEEEGNGNECVVCLSAFEE 120

Query: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTA 168
           GEEVKKLPRC HMFHA CIDMWL+SH DCPLCRAPV  P L +R TA
Sbjct: 121 GEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTA 165

[Close]

BLAST of Carg21937-RA vs. NCBI nr
Match: XP_023516728.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 265.4 bits (677), Expect = 3.4e-67
Identity = 132/166 (79.52%), Postives = 142/166 (85.54%), Query Frame = 0


Query: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60
           M DSAS + AQPT+HPAKSSLPMLYYGLVVIGTA IVLVLYN I+I WCSD+  G+ASPA
Sbjct: 1   MSDSASNYFAQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120
           LAG+YAEMVNSSRS QSSNRNLL SFKFKKGS A AAA A +EEEG GNEC VCLSAFE+
Sbjct: 61  LAGNYAEMVNSSRSLQSSNRNLLCSFKFKKGS-AAAAAAAVVEEEGNGNECVVCLSAFEE 120

Query: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHT 167
           GEEVKKLPRC HMFHA CIDMWL+SH DCPLCRAPV  P L +R T
Sbjct: 121 GEEVKKLPRCMHMFHARCIDMWLFSHCDCPLCRAPVAVPGLSKRQT 165

[Close]

BLAST of Carg21937-RA vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 5.8e-17
Identity = 54/159 (33.96%), Postives = 80/159 (50.31%), Query Frame = 0


Query: 7   TFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPAL----- 66
           +F     S  + SS   L   L+ I T+ ++LV Y  +I K+C       +S  L     
Sbjct: 40  SFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHN 99

Query: 67  -AGSYAEMVNSSRSFQSSNRNLLSS---FKFKKGSMATAAATATIEEEGI--GNECAVCL 126
             G ++     S +    N +++ S   +K+K G             +G   G++C+VCL
Sbjct: 100 GEGFFSSTQRISTNGDGLNESMIKSITVYKYKSG-------------DGFVDGSDCSVCL 159

Query: 127 SAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRA 155
           S FE+ E ++ LP+C H FH PCID WL SHS+CPLCRA
Sbjct: 160 SEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRA 185

[Close]

BLAST of Carg21937-RA vs. ExPASy Swiss-Prot
Match: Q9LRB7 (E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.1 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.9e-16
Identity = 63/172 (36.63%), Postives = 83/172 (48.26%), Query Frame = 0


Query: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIV---LVLYNLIIIKWCSDDSHGQA 60
           M +S+S+    P S PA     M   G+  +    IV   L L  +++  +C +      
Sbjct: 12  MDESSSSSSPSPVSAPA-GQAAMTAGGIATVAAVLIVFAALTLAFVLLQCYCDERRRAVT 71

Query: 61  SPALAGSYAEMVNSSRSFQSS--------NRNLLSSFKFKKGSMATAAATATIEEE--GI 120
           + + +G         RS            +  +L S      S +TAAA A  EEE    
Sbjct: 72  TTSTSGRGRRPRPRRRSGSGGDGGTGGGVDPEVLRSLPVTVYSRSTAAAAAKEEEEEDDD 131

Query: 121 GNECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAP 160
           G ECAVCL+  EDGEE + LPRC H FHA C+DMWL SHS CPLCR  V+ P
Sbjct: 132 GVECAVCLAELEDGEEARFLPRCGHGFHAECVDMWLGSHSTCPLCRLTVVVP 182

[Close]

BLAST of Carg21937-RA vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.5e-14
Identity = 33/55 (60.00%), Postives = 42/55 (76.36%), Query Frame = 0


Query: 105 EGI--GNECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVM 158
           EGI  G EC+VCL+ FE+ E ++ LP+C+H FH  CID WL SH +CPLCRAPV+
Sbjct: 127 EGIIDGTECSVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVL 181

[Close]

BLAST of Carg21937-RA vs. ExPASy Swiss-Prot
Match: O22755 (Probable E3 ubiquitin-protein ligase ATL44 OS=Arabidopsis thaliana OX=3702 GN=ATL44 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-13
Identity = 36/79 (45.57%), Postives = 45/79 (56.96%), Query Frame = 0


Query: 94  ATAAATATIEEEGIGNECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCR 153
           A  + +    EEG   ECA+CL+ F DGEE++ LP C H FH  CID WL S S CP CR
Sbjct: 85  AAESTSGAAAEEGDSTECAICLTDFADGEEIRVLPLCGHSFHVECIDKWLVSRSSCPSCR 144

Query: 154 APVMAPALCQRHTAEAAAE 173
             ++ P  C R    + AE
Sbjct: 145 R-ILTPVRCDRCGHASTAE 162

[Close]

BLAST of Carg21937-RA vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-13
Identity = 48/163 (29.45%), Postives = 75/163 (46.01%), Query Frame = 0


Query: 7   TFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPALAGSYA 66
           +F        + S    L   L+ I  +  +LV Y  +I K+C    H  +S + A    
Sbjct: 42  SFYLDDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAA--I 101

Query: 67  EMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEE-------------EGI--GNEC 126
             ++S  ++Q +N N  +       ++         E              +G    ++C
Sbjct: 102 NRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDC 161

Query: 127 AVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRA 155
           +VCLS F++ E ++ LP+C H FH PCID WL SHS+CPLCRA
Sbjct: 162 SVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRA 202

[Close]

BLAST of Carg21937-RA vs. ExPASy TrEMBL
Match: A0A6J1EGS5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434176 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.3e-88
Identity = 167/168 (99.40%), Postives = 167/168 (99.40%), Query Frame = 0


Query: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60
           MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQAS A
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASTA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120
           LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120

Query: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAE 169
           GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAE
Sbjct: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAE 168

[Close]

BLAST of Carg21937-RA vs. ExPASy TrEMBL
Match: A0A6J1HZG2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467629 PE=3 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.7e-86
Identity = 166/172 (96.51%), Postives = 167/172 (97.09%), Query Frame = 0


Query: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60
           MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSD+SHGQASPA
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDNSHGQASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120
           LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGS  TA ATATIEEEGIGNECAVCLSAFED
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGS--TAKATATIEEEGIGNECAVCLSAFED 120

Query: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAEAAAE 173
           GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAP MAPALCQRHTAE AAE
Sbjct: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPAMAPALCQRHTAEDAAE 170

[Close]

BLAST of Carg21937-RA vs. ExPASy TrEMBL
Match: A0A6J1H7W2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461337 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.8e-67
Identity = 134/167 (80.24%), Postives = 141/167 (84.43%), Query Frame = 0


Query: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60
           M DSAS + AQPTSHPAKSSLPMLYYGLVVIGTA IVLVLYN I+I WCSD+  G+ASPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120
           LAGSYAEMVNSSRS QSSNRNLL SFKFKKGS A AAA    EEEG GNEC VCLSAFE+
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAAAA----EEEGNGNECVVCLSAFEE 120

Query: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTA 168
           GEEVKKLPRC HMFHA CIDMWL+SH DCPLCRAPV  P L +R TA
Sbjct: 121 GEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQTA 163

[Close]

BLAST of Carg21937-RA vs. ExPASy TrEMBL
Match: A0A6J1JJ05 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485020 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 9.2e-66
Identity = 130/167 (77.84%), Postives = 139/167 (83.23%), Query Frame = 0


Query: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60
           M DSAS   +QPT+HPAKSSLPMLYYGLVVIGTA IVLVLYN I+I WCSD+  G+ASPA
Sbjct: 1   MSDSASNHFSQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFED 120
           LAGSYAEMVNSSRS QSSNRNLL SFKFKKGS     A A +EEEG GNEC VCLSAFE+
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGS-----AAAAVEEEGNGNECVVCLSAFEE 120

Query: 121 GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTA 168
           GEEVKKLPRC HMFHA CIDMWL+SH DCPLCRAPV  P L +R TA
Sbjct: 121 GEEVKKLPRCLHMFHARCIDMWLFSHCDCPLCRAPVAVPGLSKRQTA 162

[Close]

BLAST of Carg21937-RA vs. ExPASy TrEMBL
Match: A0A6J1D0Z7 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016600 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.9e-63
Identity = 130/170 (76.47%), Postives = 142/170 (83.53%), Query Frame = 0


Query: 4   SASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPALAG 63
           S+ST   QPT HPAKSSLPMLYYGLVV+GTA IVLV+YN I+I+WCS D  G+ SP +A 
Sbjct: 6   SSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNFIVIRWCS-DRRGRGSPGMAR 65

Query: 64  SYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEEEGIGNECAVCLSAFEDGEE 123
           SYAEM++SS SFQSS+RNLLSSF+FKKG   T AA AT EEEG G ECAVCLSAFEDGEE
Sbjct: 66  SYAEMMSSSGSFQSSSRNLLSSFRFKKG---TKAAAATAEEEGNGLECAVCLSAFEDGEE 125

Query: 124 VKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPV-MAPALCQRHTAEAAAE 173
           VKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPV +AP LCQ HTA    E
Sbjct: 126 VKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAPGLCQHHTAAEQPE 171

[Close]

BLAST of Carg21937-RA vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 4.1e-18
Identity = 54/159 (33.96%), Postives = 80/159 (50.31%), Query Frame = 0


Query: 7   TFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPAL----- 66
           +F     S  + SS   L   L+ I T+ ++LV Y  +I K+C       +S  L     
Sbjct: 40  SFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHN 99

Query: 67  -AGSYAEMVNSSRSFQSSNRNLLSS---FKFKKGSMATAAATATIEEEGI--GNECAVCL 126
             G ++     S +    N +++ S   +K+K G             +G   G++C+VCL
Sbjct: 100 GEGFFSSTQRISTNGDGLNESMIKSITVYKYKSG-------------DGFVDGSDCSVCL 159

Query: 127 SAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRA 155
           S FE+ E ++ LP+C H FH PCID WL SHS+CPLCRA
Sbjct: 160 SEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRA 185

[Close]

BLAST of Carg21937-RA vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 2.5e-15
Identity = 33/55 (60.00%), Postives = 42/55 (76.36%), Query Frame = 0


Query: 105 EGI--GNECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVM 158
           EGI  G EC+VCL+ FE+ E ++ LP+C+H FH  CID WL SH +CPLCRAPV+
Sbjct: 127 EGIIDGTECSVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVL 181

[Close]

BLAST of Carg21937-RA vs. TAIR 10
Match: AT2G17450.1 (RING-H2 finger A3A )

HSP 1 Score: 77.8 bits (190), Expect = 9.5e-15
Identity = 36/79 (45.57%), Postives = 45/79 (56.96%), Query Frame = 0


Query: 94  ATAAATATIEEEGIGNECAVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCR 153
           A  + +    EEG   ECA+CL+ F DGEE++ LP C H FH  CID WL S S CP CR
Sbjct: 85  AAESTSGAAAEEGDSTECAICLTDFADGEEIRVLPLCGHSFHVECIDKWLVSRSSCPSCR 144

Query: 154 APVMAPALCQRHTAEAAAE 173
             ++ P  C R    + AE
Sbjct: 145 R-ILTPVRCDRCGHASTAE 162

[Close]

BLAST of Carg21937-RA vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 9.5e-15
Identity = 48/163 (29.45%), Postives = 75/163 (46.01%), Query Frame = 0


Query: 7   TFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPALAGSYA 66
           +F        + S    L   L+ I  +  +LV Y  +I K+C    H  +S + A    
Sbjct: 42  SFYLDDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAA--I 101

Query: 67  EMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATIEE-------------EGI--GNEC 126
             ++S  ++Q +N N  +       ++         E              +G    ++C
Sbjct: 102 NRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDC 161

Query: 127 AVCLSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRA 155
           +VCLS F++ E ++ LP+C H FH PCID WL SHS+CPLCRA
Sbjct: 162 SVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRA 202

[Close]

BLAST of Carg21937-RA vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.5 bits (184), Expect = 4.7e-14
Identity = 52/169 (30.77%), Postives = 79/169 (46.75%), Query Frame = 0


Query: 14  SHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPALAGSYAEM-VNSS 73
           S+P  S L +  +G   I     +L  Y  ++ K+C++D+  +A+     S   + VNS 
Sbjct: 54  SNPNLSPLVIAIFG---IFATAFLLAAYYTLVSKYCANDTTNEAASESGRSDIILDVNSP 113

Query: 74  R--------SFQSSNRNL-------LSSFKFKKGSMATAAATATIEEEGI---GNECAVC 133
                    + +SS   L       +  FK KK             + G    G +C++C
Sbjct: 114 ERGDQDDPFALESSTAGLDDTLIKKIGFFKLKK------------HQNGFKINGTDCSIC 173

Query: 134 LSAFEDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQ 164
           L  F + E ++ LP+C H FH  CID WL SHS+CPLCRA ++ P   Q
Sbjct: 174 LGEFNEDESLRLLPKCNHTFHVVCIDRWLKSHSNCPLCRAKIIVPTTQQ 207

The following BLAST results are available for this feature:

BLAST of Carg21937-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6588064.1	7.6e-91	100.00	RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_022927302.1	2.7e-88	99.40	E3 ubiquitin-protein ligase EL5-like [Cucurbita moschata]	[more]
XP_022968369.1	5.7e-86	96.51	RING-H2 finger protein ATL52-like [Cucurbita maxima]	[more]
KAG6590024.1	9.1e-68	80.84	RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. sororia] >KA...	[more]
XP_023516728.1	3.4e-67	79.52	E3 ubiquitin-protein ligase EL5-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg21937-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LF64	5.8e-17	33.96	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
Q9LRB7	2.9e-16	36.63	E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5....	[more]
Q9LX93	3.5e-14	60.00	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
O22755	1.3e-13	45.57	Probable E3 ubiquitin-protein ligase ATL44 OS=Arabidopsis thaliana OX=3702 GN=AT...	[more]
Q9SRQ8	1.3e-13	29.45	RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2	[more]

BLAST of Carg21937-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EGS5	1.3e-88	99.40	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434176...	[more]
A0A6J1HZG2	2.7e-86	96.51	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467629 P...	[more]
A0A6J1H7W2	2.8e-67	80.24	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461337...	[more]
A0A6J1JJ05	9.2e-66	77.84	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485020 P...	[more]
A0A6J1D0Z7	1.9e-63	76.47	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101660...	[more]

BLAST of Carg21937-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17600.1	4.1e-18	33.96	RING/U-box superfamily protein	[more]
AT5G10380.1	2.5e-15	60.00	RING/U-box superfamily protein	[more]
AT2G17450.1	9.5e-15	45.57	RING-H2 finger A3A	[more]
AT3G03550.1	9.5e-15	29.45	RING/U-box superfamily protein	[more]
AT4G17905.1	4.7e-14	30.77	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 111..152 e-value: 8.7E-6 score: 35.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 109..153 e-value: 4.2E-13 score: 49.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 111..153 score: 12.076194
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 65..159 e-value: 1.8E-20 score: 74.4
None	No IPR available	PANTHER	PTHR45676:SF84	RING-H2 FINGER PROTEIN ATL52-LIKE	coord: 7..168
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 103..159
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 7..168

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg21937	Carg21937	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg21937-RA:1	Carg21937-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg21937-RA:cds	Carg21937-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg21937-RA	Carg21937-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane