Carg21509-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21509-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionSquamosa promoter-binding-like protein
LocationCarg_Chr13: 7238203 .. 7240398 (-)
Sequence length989
RNA-Seq ExpressionCarg21509-RA
SyntenyCarg21509-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACCGGAAAAGCCGACGGAAAAAGGAGGCTGAACTACTACGACATGGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGTGGAGGTTGTTGATGGAGAATTAGGGTTTGGTGGGGATTTCAATAAGAAGAAGAAGACTCTGTGTTCTGGTGGTGGTTCTAATAAAAAATCAGGATCTGGATACGGTGGAGCGGCTACGGCGGTGCCGTCGTGCCAGGCGGATAATTGCAATGCCGATCTGTCTAGTTCCAAGCGCTACCACTGCCGCCATAAGGTCTGTGAGTTTCATGCCAAGGCTCTGGCGGTTATGGTGGCCGGAATCCACCAACGGTTCTGCCAACAATGCAGCAGGTATTTTGGGACAAAAACCCTAATCTTAGATCTGTTTTTTTAATTATGTGGTTTAACCTATTTTAAAGGTAACGTAAAGATTAGATAATAATTCAGCATATAGTTAGTGAGATAATTAGTATATGTTTTAACTGTTCTTCAATTAATTAACTCCAAAATTTAACTGTTTTCAGTGTAATTTTCTTCATCTCTTTGGAGTTTGGTTCATCCAAAAATAAATTACTGTTTATTCATCCCAAGACCATCAACTATGAAATAAATGAAGAAAATTGTAATTATAATAATGTAAAAATGGTTTATGTATTTATTTATTCATTCATTCATTTTTCTACTTACTCAAGTTTTTGAAAAAAATAAAAAAATAAATCAGTCTTGACCCCCTAAATTTGGCAAGGTTTTTCAATTTTTTTTTTTTAAACTTTTAATTTTTTTAAAAATGGAACCCTAAATTTAATTAAATGTTGAAATTTTTATTTATTTATTTTTTCACTCATATAAGCACAAAATTGTACAGTTCAATAAAATTTTCAAATTTTACGAGTTTTAACAAACTTTAGCTCTAATGTTCGTAATTTTTTGTATAACAATTCTTCGAAAGTGAATAGAAGGTAAATTTTGGTATTCTTATTTAAATTTAAGATAAAGATAGTAACATTTATTCGAGATGTCTTGAGTTCCTAAACATTAATGTAAATAGATAGTTTTTTCACAAAATTTTATTATAATCTTTACTATGGTAGAGAAATATATTTTATGAGGAACAAAATTTTACTATATAGTTTTAAATTTGATAATTTTAGGATACTAGAGAAAAGTTTTGAATCCTAACTTTAAATATAAAATTTTAACATAAAACAAACTTTGATATATTAATTATGAGAGTAAAATAAAGAATAACAATAATAATAATAGTAATAATAATGTAGTTTGAATATATAAATACTCTCTTTGTGAATCTTCATCAGTGGTACAATTGCTTAATTTATTGGTTTTTCAGGATTTGGTTGCGTTTGAAATCCATTTTAGTCTATATATCTCGAGAATAATACGTGATCTAAAGATAGATGTCTTAATTGTTGAACGATACGTTCTTTTAGGGTTTAAATAGTTTTATATAGTGTTACATATTCGTGCATATTTATGATTGTTTATATGTAGTAGTGATCGTTATTGAGAAACTAAATATCCATCATTTGGGTTCCTGGAGATTTCATGGTCTACAAGAGTTCGATGAAACAAAAAGGAGTTGCCGGAAGCGGTTGGCCGGACACAACCAGCGGCGGCGGAAGAGCTCATCGGATGTCCATGGCGGAACCTCAACCTAGCAATATGCAGAATGATGAAAGAAGGGGCATTTTGTTTTTAATTTTGTTGGTCATTGCTTCCATTGTTACCAGATTTGCTTGCTCTCTCTCTTCTGTCAATCCAGCCAGCCTTTTGCTTATGCTCTGTTCTACAGACCAATTAGTTCATCTTTTGTATTAATCCCTTTGCAATGTGAAGCAAGATTATGGAGCATATCAGCTTTTGATTTACTTTCAATCTAATTCAACGAAACAATAAGGAATGAATGTCTACAATCAATAGCAGGACCATCAAAACATATACTCTAAAGAAACAACATGAAATTTGACGAGGCGAGGAAACAGTTGAGTTAATCTCGGGCGATACCGAGAACTTTCATTGCTAGCTCCCAGCAGACGATTGCGGCTGCATTGGCAGGAAATGCTCGAAGTATGGTTGGACCTAATCCTGTATAGCATCCTCTAATTCCAGCGTTTTGGTATATCTAGAACAATGATTTGTTTCAGCATGTTGTATAATTTTGAGCCAGAGATGAGAA

mRNA sequence

ATGGCCACCGGAAAAGCCGACGGAAAAAGGAGGCTGAACTACTACGACATGGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGTGGAGGTTGTTGATGGAGAATTAGGGTTTGGTGGGGATTTCAATAAGAAGAAGAAGACTCTGTGTTCTGGTGGTGGTTCTAATAAAAAATCAGGATCTGGATACGGTGGAGCGGCTACGGCGGTGCCGTCGTGCCAGGCGGATAATTGCAATGCCGATCTGTCTAGTTCCAAGCGCTACCACTGCCGCCATAAGGTCTGTGAGTTTCATGCCAAGGCTCTGGCGGTTATGGTGGCCGGAATCCACCAACGGTTCTGCCAACAATGCAGCAGATTTCATGGTCTACAAGAGTTCGATGAAACAAAAAGGAGTTGCCGGAAGCGGTTGGCCGGACACAACCAGCGGCGGCGGAAGAGCTCATCGGATGTCCATGGCGGAACCTCAACCTAGCAATATGCAGAATGATGAAAGAAGGGGCATTTTGTTTTTAATTTTGTTGGTCATTGCTTCCATTGTTACCAGATTTGCTTGCTCTCTCTCTTCTGTCAATCCAGCCAGCCTTTTGCTTATGCTCTGTTCTACAGACCAATTAGTTCATCTTTTGTATTAATCCCTTTGCAATGTGAAGCAAGATTATGGAGCATATCAGCTTTTGATTTACTTTCAATCTAATTCAACGAAACAATAAGGAATGAATGTCTACAATCAATAGCAGGACCATCAAAACATATACTCTAAAGAAACAACATGAAATTTGACGAGGCGAGGAAACAGTTGAGTTAATCTCGGGCGATACCGAGAACTTTCATTGCTAGCTCCCAGCAGACGATTGCGGCTGCATTGGCAGGAAATGCTCGAAGTATGGTTGGACCTAATCCTGTATAGCATCCTCTAATTCCAGCGTTTTGGTATATCTAGAACAATGATTTGTTTCAGCATGTTGTATAATTTTGAGCCAGAGATGAGAA

Coding sequence (CDS)

ATGGCCACCGGAAAAGCCGACGGAAAAAGGAGGCTGAACTACTACGACATGGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGTGGAGGTTGTTGATGGAGAATTAGGGTTTGGTGGGGATTTCAATAAGAAGAAGAAGACTCTGTGTTCTGGTGGTGGTTCTAATAAAAAATCAGGATCTGGATACGGTGGAGCGGCTACGGCGGTGCCGTCGTGCCAGGCGGATAATTGCAATGCCGATCTGTCTAGTTCCAAGCGCTACCACTGCCGCCATAAGGTCTGTGAGTTTCATGCCAAGGCTCTGGCGGTTATGGTGGCCGGAATCCACCAACGGTTCTGCCAACAATGCAGCAGATTTCATGGTCTACAAGAGTTCGATGAAACAAAAAGGAGTTGCCGGAAGCGGTTGGCCGGACACAACCAGCGGCGGCGGAAGAGCTCATCGGATGTCCATGGCGGAACCTCAACCTAG

Protein sequence

MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGSGYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFHGLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST
Homology
BLAST of Carg21509-RA vs. NCBI nr
Match: KAG7019608.1 (Squamosa promoter-binding protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 310.8 bits (795), Expect = 6.5e-81
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60
           MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS
Sbjct: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60

Query: 61  GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120
           GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH
Sbjct: 61  GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120

Query: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 157
           GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST
Sbjct: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 156

BLAST of Carg21509-RA vs. NCBI nr
Match: XP_022927387.1 (squamosa promoter-binding-like protein 3 isoform X1 [Cucurbita moschata] >KAG6583985.1 Squamosa promoter-binding protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 309.3 bits (791), Expect = 1.9e-80
Identity = 155/156 (99.36%), Postives = 155/156 (99.36%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60
           MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS
Sbjct: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60

Query: 61  GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120
           GYGGAATA PSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH
Sbjct: 61  GYGGAATAAPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120

Query: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 157
           GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST
Sbjct: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 156

BLAST of Carg21509-RA vs. NCBI nr
Match: XP_023001757.1 (squamosa promoter-binding-like protein 3 [Cucurbita maxima])

HSP 1 Score: 299.3 bits (765), Expect = 2.0e-77
Identity = 153/156 (98.08%), Postives = 153/156 (98.08%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60
           MATGKADGKRRLNYYDM EEEEEEEEEVEVVDGELGFGGDFNKKKKTL SGGGSNKKSGS
Sbjct: 1   MATGKADGKRRLNYYDM-EEEEEEEEEVEVVDGELGFGGDFNKKKKTLSSGGGSNKKSGS 60

Query: 61  GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120
           GYGGAATA PSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH
Sbjct: 61  GYGGAATAAPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120

Query: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 157
           GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST
Sbjct: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 155

BLAST of Carg21509-RA vs. NCBI nr
Match: XP_023520290.1 (squamosa promoter-binding-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 295.8 bits (756), Expect = 2.2e-76
Identity = 151/156 (96.79%), Postives = 152/156 (97.44%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60
           MATGKADGKRRL+YYDM EEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS
Sbjct: 1   MATGKADGKRRLSYYDM-EEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60

Query: 61  GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120
           G GG ATA PSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH
Sbjct: 61  GCGGEATAAPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120

Query: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 157
           GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST
Sbjct: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 155

BLAST of Carg21509-RA vs. NCBI nr
Match: XP_038894994.1 (squamosa promoter-binding-like protein 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 251.5 bits (641), Expect = 4.7e-63
Identity = 135/164 (82.32%), Postives = 139/164 (84.76%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVE--------VVDGELGFGGDFNKKKKTLCSGG 60
           MATGKADGKRRLNYY+MEEEEEE+EEE E         + GELGF GDFNKKKK L SG 
Sbjct: 1   MATGKADGKRRLNYYEMEEEEEEDEEEEEEEEDHEEQQIHGELGFAGDFNKKKKILTSGT 60

Query: 61  GSNKKSGSGYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRF 120
            SNKKSGSGYGGAA A PSCQADNCNADLS SKRYH RHKVCEFHAKA AV VAGIHQRF
Sbjct: 61  SSNKKSGSGYGGAA-AAPSCQADNCNADLSGSKRYHRRHKVCEFHAKAPAVAVAGIHQRF 120

Query: 121 CQQCSRFHGLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 157
           CQQCSRFHGLQEFDE KRSCRKRLAGHNQRRRKSSSDVHG + T
Sbjct: 121 CQQCSRFHGLQEFDEAKRSCRKRLAGHNQRRRKSSSDVHGESPT 163

BLAST of Carg21509-RA vs. ExPASy Swiss-Prot
Match: P93015 (Squamosa promoter-binding-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=SPL3 PE=1 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 1.7e-31
Identity = 76/149 (51.01%), Postives = 96/149 (64.43%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60
           M   KA+GKR L     EEEEEEE E+ +  + E        KK+K              
Sbjct: 3   MRRSKAEGKRSLRELSEEEEEEEETEDEDTFEEEEA----LEKKQK-------------- 62

Query: 61  GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120
             G A ++   CQ ++C AD+S +K+YH RHKVC+FHAKA  V ++G+HQRFCQQCSRFH
Sbjct: 63  --GKATSSSGVCQVESCTADMSKAKQYHKRHKVCQFHAKAPHVRISGLHQRFCQQCSRFH 122

Query: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSD 150
            L EFDE KRSCR+RLAGHN+RRRKS++D
Sbjct: 123 ALSEFDEAKRSCRRRLAGHNERRRKSTTD 131

BLAST of Carg21509-RA vs. ExPASy Swiss-Prot
Match: Q38740 (Squamosa promoter-binding protein 2 OS=Antirrhinum majus OX=4151 GN=SBP2 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.4e-30
Identity = 81/150 (54.00%), Postives = 99/150 (66.00%), Query Frame = 0

Query: 5   KADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGSGY-- 64
           K  G    ++ + EE E+EEEEE E V  ++ F  +   KKK L  G GS  KSG  +  
Sbjct: 17  KLVGDEGSDFEEEEEGEDEEEEEQERV-VKVNF-AESQLKKKKLNLGEGSGGKSGEKHTA 76

Query: 65  -GGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFHG 124
            GG   A P C  +NC ADL + K+Y+ RH+VCE HAKA  V V G+ QRFCQQCSRFH 
Sbjct: 77  SGGGVVAQPCCLVENCGADLRNCKKYYQRHRVCEVHAKAPVVSVEGLMQRFCQQCSRFHD 136

Query: 125 LQEFDETKRSCRKRLAGHNQRRRKSSSDVH 152
           L EFD+TKRSCR+RLAGHN+RRRKSS + H
Sbjct: 137 LSEFDQTKRSCRRRLAGHNERRRKSSLESH 164

BLAST of Carg21509-RA vs. ExPASy Swiss-Prot
Match: Q38741 (Squamosa promoter-binding protein 1 OS=Antirrhinum majus OX=4151 GN=SBP1 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.6e-29
Identity = 75/151 (49.67%), Postives = 96/151 (63.58%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60
           M T K +GKR +     +E+ EEE++            G+ +KK + L   G    K  S
Sbjct: 1   MDTSKGEGKRVIKLPGSQEQGEEEDDI-----------GEDSKKTRALTPSG----KRAS 60

Query: 61  GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120
           G     +   SCQ +NC A+++++K YH RHKVCEFHAKA  V+ +G+ QRFCQQCSRFH
Sbjct: 61  G-----STQRSCQVENCAAEMTNAKPYHRRHKVCEFHAKAPVVLHSGLQQRFCQQCSRFH 120

Query: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVH 152
            L EFDE KRSCR+RLAGHN+RRRKSS D H
Sbjct: 121 ELSEFDEAKRSCRRRLAGHNERRRKSSHDTH 131

BLAST of Carg21509-RA vs. ExPASy Swiss-Prot
Match: A2YFT9 (Squamosa promoter-binding-like protein 10 OS=Oryza sativa subsp. indica OX=39946 GN=SPL10 PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 3.3e-27
Identity = 60/90 (66.67%), Postives = 68/90 (75.56%), Query Frame = 0

Query: 59  GSGYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSR 118
           G G+GGA    P CQA+ C ADLS +K YH RHKVCE+HAKA  V  +G  QRFCQQCSR
Sbjct: 168 GLGFGGAHHQPPRCQAEGCKADLSGAKHYHRRHKVCEYHAKASVVAASGKQQRFCQQCSR 227

Query: 119 FHGLQEFDETKRSCRKRLAGHNQRRRKSSS 149
           FH L EFDE KRSCRKRLA HN+RRRK ++
Sbjct: 228 FHVLTEFDEAKRSCRKRLAEHNRRRRKPAA 257

BLAST of Carg21509-RA vs. ExPASy Swiss-Prot
Match: Q0DAE8 (Squamosa promoter-binding-like protein 10 OS=Oryza sativa subsp. japonica OX=39947 GN=SPL10 PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 3.3e-27
Identity = 60/90 (66.67%), Postives = 68/90 (75.56%), Query Frame = 0

Query: 59  GSGYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSR 118
           G G+GGA    P CQA+ C ADLS +K YH RHKVCE+HAKA  V  +G  QRFCQQCSR
Sbjct: 168 GLGFGGAHHQPPRCQAEGCKADLSGAKHYHRRHKVCEYHAKASVVAASGKQQRFCQQCSR 227

Query: 119 FHGLQEFDETKRSCRKRLAGHNQRRRKSSS 149
           FH L EFDE KRSCRKRLA HN+RRRK ++
Sbjct: 228 FHVLTEFDEAKRSCRKRLAEHNRRRRKPAA 257

BLAST of Carg21509-RA vs. ExPASy TrEMBL
Match: A0A6J1EH14 (Squamosa promoter-binding-like protein OS=Cucurbita moschata OX=3662 GN=LOC111434224 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 9.1e-81
Identity = 155/156 (99.36%), Postives = 155/156 (99.36%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60
           MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS
Sbjct: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60

Query: 61  GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120
           GYGGAATA PSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH
Sbjct: 61  GYGGAATAAPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120

Query: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 157
           GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST
Sbjct: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 156

BLAST of Carg21509-RA vs. ExPASy TrEMBL
Match: A0A6J1KHJ1 (Squamosa promoter-binding-like protein OS=Cucurbita maxima OX=3661 GN=LOC111495800 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 9.5e-78
Identity = 153/156 (98.08%), Postives = 153/156 (98.08%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60
           MATGKADGKRRLNYYDM EEEEEEEEEVEVVDGELGFGGDFNKKKKTL SGGGSNKKSGS
Sbjct: 1   MATGKADGKRRLNYYDM-EEEEEEEEEVEVVDGELGFGGDFNKKKKTLSSGGGSNKKSGS 60

Query: 61  GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120
           GYGGAATA PSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH
Sbjct: 61  GYGGAATAAPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120

Query: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 157
           GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST
Sbjct: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 155

BLAST of Carg21509-RA vs. ExPASy TrEMBL
Match: A0A6J1K2Z2 (Squamosa promoter-binding-like protein OS=Cucurbita maxima OX=3661 GN=LOC111490194 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.1e-60
Identity = 132/158 (83.54%), Postives = 136/158 (86.08%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVV--DGELGFGGDFNKKKKTLCSGGGSNKKS 60
           MATGKADGKRRLNYYD EE+EEEEEEE E    DGELGFG DFNKKKK +  GGGSNK S
Sbjct: 1   MATGKADGKRRLNYYDTEEDEEEEEEEDEEEDGDGELGFGEDFNKKKK-IVGGGGSNKGS 60

Query: 61  GSGYGGAATA----VPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQ 120
           GSGYGGAA A     PSCQADNC+ADLS SKRYH RHKVCEFHAKA AV+VAGIHQRFCQ
Sbjct: 61  GSGYGGAAAAAVAVAPSCQADNCSADLSGSKRYHRRHKVCEFHAKAPAVVVAGIHQRFCQ 120

Query: 121 QCSRFHGLQEFDETKRSCRKRLAGHNQRRRKSSSDVHG 153
           QCSRFHGL EFD TKRSCRKRLAGHNQRRRKSSSDVHG
Sbjct: 121 QCSRFHGLMEFDLTKRSCRKRLAGHNQRRRKSSSDVHG 157

BLAST of Carg21509-RA vs. ExPASy TrEMBL
Match: A0A6J1GT00 (Squamosa promoter-binding-like protein OS=Cucurbita moschata OX=3662 GN=LOC111456848 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.3e-60
Identity = 129/160 (80.62%), Postives = 134/160 (83.75%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEV---EVVDGELGFGGDFNKKKKTLCSGGGSNKK 60
           M TGKADGKRRLNYYD+EEE++EEEEE    E  DGELGFG DFNKKKK +  GG SNK 
Sbjct: 1   MVTGKADGKRRLNYYDVEEEDDEEEEEADEEEDGDGELGFGEDFNKKKKIVDGGGSSNKG 60

Query: 61  SGSGYGGAA-----TAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRF 120
           SGSGYGGAA        PSCQADNCNADLS SKRYH RHKVCEFHAKA AV+VAGIHQRF
Sbjct: 61  SGSGYGGAAATAAVAVAPSCQADNCNADLSGSKRYHRRHKVCEFHAKAPAVVVAGIHQRF 120

Query: 121 CQQCSRFHGLQEFDETKRSCRKRLAGHNQRRRKSSSDVHG 153
           CQQCSRFHGL EFD TKRSCRKRLAGHNQRRRKSSSDVHG
Sbjct: 121 CQQCSRFHGLMEFDLTKRSCRKRLAGHNQRRRKSSSDVHG 160

BLAST of Carg21509-RA vs. ExPASy TrEMBL
Match: A0A1S3B6H6 (squamosa promoter-binding-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103486540 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.2e-60
Identity = 130/167 (77.84%), Postives = 137/167 (82.04%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVD---------GELGFGGDFNKKKKTLCSG 60
           MATGKADGKRRLNYY+MEEE+EE+EEE E  D         G LGF GDFNKKKK L SG
Sbjct: 1   MATGKADGKRRLNYYEMEEEDEEDEEEEEEEDEDEEQEIDGGGLGFSGDFNKKKKVLTSG 60

Query: 61  GGSNKKSGSGYGGA--ATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIH 120
           G SNKKSGSG+G    A A P CQADNCNADLS SKRYH RHKVCEFHAKA AV VAGI 
Sbjct: 61  GSSNKKSGSGFGTGPPAAAPPPCQADNCNADLSGSKRYHRRHKVCEFHAKAPAVAVAGID 120

Query: 121 QRFCQQCSRFHGLQEFDETKRSCRKRLAGHNQRRRKSSSDVHGGTST 157
           QRFCQQCSRFHGLQEFD+TKRSCRKRLAGHNQRRRKSSSD+HG +ST
Sbjct: 121 QRFCQQCSRFHGLQEFDDTKRSCRKRLAGHNQRRRKSSSDLHGESST 167

BLAST of Carg21509-RA vs. TAIR 10
Match: AT2G33810.1 (squamosa promoter binding protein-like 3 )

HSP 1 Score: 137.1 bits (344), Expect = 1.2e-32
Identity = 76/149 (51.01%), Postives = 96/149 (64.43%), Query Frame = 0

Query: 1   MATGKADGKRRLNYYDMEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGS 60
           M   KA+GKR L     EEEEEEE E+ +  + E        KK+K              
Sbjct: 3   MRRSKAEGKRSLRELSEEEEEEEETEDEDTFEEEEA----LEKKQK-------------- 62

Query: 61  GYGGAATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFH 120
             G A ++   CQ ++C AD+S +K+YH RHKVC+FHAKA  V ++G+HQRFCQQCSRFH
Sbjct: 63  --GKATSSSGVCQVESCTADMSKAKQYHKRHKVCQFHAKAPHVRISGLHQRFCQQCSRFH 122

Query: 121 GLQEFDETKRSCRKRLAGHNQRRRKSSSD 150
            L EFDE KRSCR+RLAGHN+RRRKS++D
Sbjct: 123 ALSEFDEAKRSCRRRLAGHNERRRKSTTD 131

BLAST of Carg21509-RA vs. TAIR 10
Match: AT3G15270.1 (squamosa promoter binding protein-like 5 )

HSP 1 Score: 122.5 bits (306), Expect = 3.0e-28
Identity = 70/131 (53.44%), Postives = 84/131 (64.12%), Query Frame = 0

Query: 24  EEEEVEVVDGELGFGGDFNKKKKTLCSGGGSNKKSGSGYGGAATAVPS--CQADNCNADL 83
           EEE    +DGE   GGD +K+KK +              G +   VPS  CQ D C  +L
Sbjct: 23  EEEMENGMDGEEEDGGDEDKRKKVM----------ERVRGPSTDRVPSRLCQVDRCTVNL 82

Query: 84  SSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFHGLQEFDETKRSCRKRLAGHNQ 143
           + +K+Y+ RH+VCE HAKA A  VAG+ QRFCQQCSRFH L EFDE KRSCR+RLAGHN+
Sbjct: 83  TEAKQYYRRHRVCEVHAKASAATVAGVRQRFCQQCSRFHELPEFDEAKRSCRRRLAGHNE 142

Query: 144 RRRKSSSDVHG 153
           RRRK S D  G
Sbjct: 143 RRRKISGDSFG 143

BLAST of Carg21509-RA vs. TAIR 10
Match: AT1G02065.1 (squamosa promoter binding protein-like 8 )

HSP 1 Score: 120.2 bits (300), Expect = 1.5e-27
Identity = 60/92 (65.22%), Postives = 70/92 (76.09%), Query Frame = 0

Query: 56  KKSGSGYGGAATAV--PSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFC 115
           ++S  G  G A ++  P CQA+ CNADLS +K YH RHKVCEFH+KA  V+ AG+ QRFC
Sbjct: 170 RRSRPGESGMANSLSTPRCQAEGCNADLSHAKHYHRRHKVCEFHSKASTVVAAGLSQRFC 229

Query: 116 QQCSRFHGLQEFDETKRSCRKRLAGHNQRRRK 146
           QQCSRFH L EFD  KRSCRKRLA HN+RRRK
Sbjct: 230 QQCSRFHLLSEFDNGKRSCRKRLADHNRRRRK 261

BLAST of Carg21509-RA vs. TAIR 10
Match: AT3G60030.1 (squamosa promoter-binding protein-like 12 )

HSP 1 Score: 118.6 bits (296), Expect = 4.4e-27
Identity = 75/144 (52.08%), Postives = 91/144 (63.19%), Query Frame = 0

Query: 17  MEEEEEEEEEEVEVVDGELGFGGDFNKKKKTLCSGG----GSNKKSGSGYGGAATAVPSC 76
           +E E++++   V VV  E     D +  + TL  GG    G+  K     GG  +    C
Sbjct: 68  IEMEKKKKRRAVTVVAMEEDNLKDDDAHRLTLNLGGNNIEGNGVKKTKLGGGIPSRAICC 127

Query: 77  QADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFHGLQEFDETKRSC 136
           Q DNC ADLS  K YH RHKVCE H+KA   +V GI QRFCQQCSRFH L+EFDE KRSC
Sbjct: 128 QVDNCGADLSKVKDYHRRHKVCEIHSKATTALVGGIMQRFCQQCSRFHVLEEFDEGKRSC 187

Query: 137 RKRLAGHNQRRRKSSSDVHG-GTS 156
           R+RLAGHN+RRRK++ D  G GTS
Sbjct: 188 RRRLAGHNKRRRKANPDTIGNGTS 211

BLAST of Carg21509-RA vs. TAIR 10
Match: AT1G53160.2 (squamosa promoter binding protein-like 4 )

HSP 1 Score: 115.5 bits (288), Expect = 3.7e-26
Identity = 69/144 (47.92%), Postives = 89/144 (61.81%), Query Frame = 0

Query: 7   DGKRRLNYYDMEEEEEEEEEEVEV-VDGELGFGGDFNKKKKTLCSGGGSNKKSGSGYGGA 66
           +GKR      M+++    EE++E   D E   G D  +  +   + GGS           
Sbjct: 2   EGKRSQGQGYMKKKSYLVEEDMETDTDEEEEVGRDRVRGSRGSINRGGS----------- 61

Query: 67  ATAVPSCQADNCNADLSSSKRYHCRHKVCEFHAKALAVMVAGIHQRFCQQCSRFHGLQEF 126
              +  CQ D C AD+  +K YH RHKVCE HAKA +V ++G++QRFCQQCSRFH LQEF
Sbjct: 62  ---LRLCQVDRCTADMKEAKLYHRRHKVCEVHAKASSVFLSGLNQRFCQQCSRFHDLQEF 121

Query: 127 DETKRSCRKRLAGHNQRRRKSSSD 150
           DE KRSCR+RLAGHN+RRRKSS +
Sbjct: 122 DEAKRSCRRRLAGHNERRRKSSGE 131

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019608.16.5e-81100.00Squamosa promoter-binding protein 1, partial [Cucurbita argyrosperma subsp. argy... [more]
XP_022927387.11.9e-8099.36squamosa promoter-binding-like protein 3 isoform X1 [Cucurbita moschata] >KAG658... [more]
XP_023001757.12.0e-7798.08squamosa promoter-binding-like protein 3 [Cucurbita maxima][more]
XP_023520290.12.2e-7696.79squamosa promoter-binding-like protein 3 [Cucurbita pepo subsp. pepo][more]
XP_038894994.14.7e-6382.32squamosa promoter-binding-like protein 3 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P930151.7e-3151.01Squamosa promoter-binding-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=SPL3... [more]
Q387401.4e-3054.00Squamosa promoter-binding protein 2 OS=Antirrhinum majus OX=4151 GN=SBP2 PE=2 SV... [more]
Q387414.6e-2949.67Squamosa promoter-binding protein 1 OS=Antirrhinum majus OX=4151 GN=SBP1 PE=2 SV... [more]
A2YFT93.3e-2766.67Squamosa promoter-binding-like protein 10 OS=Oryza sativa subsp. indica OX=39946... [more]
Q0DAE83.3e-2766.67Squamosa promoter-binding-like protein 10 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A6J1EH149.1e-8199.36Squamosa promoter-binding-like protein OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1KHJ19.5e-7898.08Squamosa promoter-binding-like protein OS=Cucurbita maxima OX=3661 GN=LOC1114958... [more]
A0A6J1K2Z21.1e-6083.54Squamosa promoter-binding-like protein OS=Cucurbita maxima OX=3661 GN=LOC1114901... [more]
A0A6J1GT002.3e-6080.63Squamosa promoter-binding-like protein OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A1S3B6H65.2e-6077.84squamosa promoter-binding-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103486540... [more]
Match NameE-valueIdentityDescription
AT2G33810.11.2e-3251.01squamosa promoter binding protein-like 3 [more]
AT3G15270.13.0e-2853.44squamosa promoter binding protein-like 5 [more]
AT1G02065.11.5e-2765.22squamosa promoter binding protein-like 8 [more]
AT3G60030.14.4e-2752.08squamosa promoter-binding protein-like 12 [more]
AT1G53160.23.7e-2647.92squamosa promoter binding protein-like 4 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004333SBP domainPFAMPF03110SBPcoord: 72..145
e-value: 8.0E-32
score: 109.4
IPR004333SBP domainPROSITEPS51141ZF_SBPcoord: 69..146
score: 31.188313
IPR036893SBP domain superfamilyGENE3D4.10.1100.10coord: 63..133
e-value: 8.7E-32
score: 111.1
IPR036893SBP domain superfamilySUPERFAMILY103612SBT domaincoord: 70..150
IPR017238Squamosa promoter-binding proteinPIRSFPIRSF037575SBPcoord: 3..156
e-value: 9.5E-54
score: 179.4
NoneNo IPR availablePIRSRPIRSR037575-1PIRSR037575-1coord: 64..147
e-value: 2.6E-43
score: 144.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
IPR044817Squamosa promoter-binding-like proteinPANTHERPTHR31251SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4coord: 39..152

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg21509Carg21509gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg21509-RA:2Carg21509-RA:2exon
Carg21509-RA:1Carg21509-RA:1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg21509-RA:three_prime_utrCarg21509-RA:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg21509-RA:cdsCarg21509-RA:cds_2CDS
Carg21509-RA:cdsCarg21509-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg21509-RACarg21509-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009908 flower development
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0046872 metal ion binding