Carg21212-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21212-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description60S ribosomal protein L5-like
LocationCarg_Chr18: 7077291 .. 7080573 (-)
Sequence length1134
RNA-Seq ExpressionCarg21212-RA
SyntenyCarg21212-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTCTCCATTTTTGGAGCTAACCGCAAGCATGTTCGTTGCATAATGCGATGTCTATCGATACTTCACAGGTTATTTTCCCTTATCATTTGGATTAGAAATGGTTAATATTTTATTTTAAAAATACTACAAAGTTTCATTACTACGCCTTGACTTCTTAAAAGTTTTCAAAAATATTCTTACTACGGTAAAAAATACATTTGAAATTTTAATAGTAATATTAAAACCTTTTACCAAATATAATTGTCATCAATATATGAACGAAAATCGTCTGTCAATACTTCACAGGTTATTTTCAAATTTTTTTACGTTACTTTTGAAAGGTTATGAATGTTATTGAAATGTGTTTTTTTTAGAAGGTGATTTCGAAGTTTTTATAAAAAAATTATGGGTATTAATGAAAATTTAAAAAATTTAATGATATTTTTTATTTTTCTTTTTTTTTAACGTTTAAGGGTATTTTTGGAATAAATTATTAACCATTTTTTAAAATTTTTAAAAATTTATTTTTTAAATGGATATATATATATATATATTTTATTGCTTTACACTGATTATAGGAAAGATGGGCCCGGCCCACTTTGAATAAGAAGCTGGAGTCGTCGGCCCATATGCAAGCAATCTAGGGTTTTATGCCCAACAAGAAAAAAGAAAACCTAGGGTTTATTATATAAAGGGATTCTGCCCTTTCTCTTGTTTTCCGCCACTTCTCTTTCGGGTTCCTGCTCCGCTCGCGGCACCAAATCTACAATGGTGAGAGCTCCATCCCTTTCCCTTGCGCTTTCGTTTGTTCTTGTTTCTATTGACTGGTTATACTATAGAAGTTCATGTAATCATACTTTTACTGCGTTTTCCGACTTCTCGTTCATCTTCGCAATCCGTTGTGTGGTGGAATTTATGTCATATTTTGTTAGGAACTTGTCTGTAATTCGTAGGTTTCATGCTATGTATTTGTAGGGATCTTTATTTTAAGCTATGTTGGTGGTCGGACTGTTTTTTTTAGCTTCGTTCCTAGCTTTTAGTTTGAGCATTCCGCCGCTCTTAATTATCATACATATCTTTGCTCTACAGATCAGAGATAACTTTATAGTATTTTATGGTTTTGACGGTCATTGATTCATTAAATAGTAATGCGAGGTCATATTTTTTATGTCGTGATGTGTTTACAATGTTGGGGGTAGTTATATGGTGGGTTATTTATTGTAGGCTTTTGCCAAAGCTCAGAAGACGAAGGCCTACTTCAAACGTTATCAAGTCAAGTTCAAGAGAAGGAGAGGTATTTATTCCCTATATAAATAATGTCACAATGTTATTTATTCTTTTCTAGATTTCTGATTGTTGTTTCTGAATATTCACAGAGGGAAAGACTGACTACCGAGCTAGAATTCGTCTTATTAACCAGGACAAGAACAAGTACAACACTCCCAAGTATCGCATTGTTGTCCGATTTGTATCCTATGAAAGATCTGAGTTTGAATAGTTTGTAGAAGTTTACATGTTTTGGGTGCTGAAGAGCATGAGTAATTGTTTCTCTTTTAGCTATCAAATGCAAGACTTTTTGTTGGGTTTTATTTTTAGATTACTTTCAGTTTTACATCTATTTTTCATGGATTTTGAGATGTGTGTCCTTGACATCATTCAATAGTCGAACAAAGATATCACAGCTCAGATAATTTCTGCTAGCATTGCTGGGGACTTGGTCCTTGCTTCAGCTTATTCTCATGAGCTTCCTCGTTACGGGCTGGAAGTTGGTCTCACCAACTATGCTGCAGGTATGCTTTCAATCTTGTCACTCTTTCTTGTATGTCCGTTCATTTATAATATTCCTATTAACATTTTCAATGACTTGATTAACAACTGTTTTAAAATTGTTTAGCATATTGCACTGGGCTTCTCTTGGCTCGACGAGTTTTGAAACAGCTTGAGATGGATGATGAATATGAGGGCAATGTGGAGGTACATCTATTCTTAACTATTTCCTCTACCCCTATCTATATTATATTTTTAATTTTATTAACACTATGATGTTGCACTCTTAGGCTACTGGTGAAGATTACTCTGTTGAGCCTGCTGATACCAGAAGGCCATTCCGTGCTTTACTTGATGTCGGTCTTCTTAAAACTACAACTGGAAACCGTGTTTTTGGTGCTCTTAAGGTACTCAGTTAGCAACCGGATATTCAATTTCTAGTTCTTTAAAACTTAATTTCTCTGTCAGCTCCTGTAATCAAAGTATCCTATGATGTTATCTTAAATCAACATTTTCAGATACTAATCTCAAGGAAAGCTAGTTTATTCTGATACTGACAATCTCATTGTTTGATATAAGTCGTCGAGTACACGTTTTCGAACAATCTCATTGTTCTGTACTTCTTTGGTTGAATGTTGTCATGCTATGGCTGGTTGGAATTGTTAATATGATATATTGGCCGTGTAAAAACCTGCAATGAATTTCAATTTGTAAAACACAGGGAGCTTTAGATGGTGGATTGGACATCCCTCACAGCGATAAGAGATTTGCTGGGTTTTCCAAGGATAGCAAGCAGCTTGATGCTGATGTTCATAGGAAGTACATTTATGGTGGACACGTTGCGGCGTACATGCGGGTACGACCGTGCTGTTTATTTGTTCTTATGATATGCTATTTGTATCCTTCACGTTTGATAACTTTTTATATATGGCTTCATCAGACCTTGATGGAAGATGAACCAGAGAAGTACCAAACCCACTTCAGCGAATACATCAAGAAGGGCATTGAGGCTGATGACATCGAGGGTTTATACAAGAAAGTTCATGCCGCCATTCGGGCAGACCCATCAGTGAAGAAATCCGACAAGCCCCAACCCAAGGCACACAAGAGGTAGAAACGAAGCTCGAGCTTTTGTCGTCGAGCGTAAAATATCTTTCCTATGTGAACTTTCTTTGTTGCGGGCATTGTTTATTAACATTCCCCTGTTGTTGTTGGTTGAAAATTACAGGTACAACTTGAAGAAGCTGACATACGACGAAAGAAAAGCTCGCTTGGTGGAACGTTTGAACGCTCTCAACTCTGCAGCTAATGCTGACGACGACGATGACGATGAGGATGACGAGTGAAAGATGAAAATTAGTTGATGTGGGCATGGAAGGATTTTAATGTAGTAGTTCTGCTATTTATTATTTTAAGTTTCGTTCTTTGGTCGAAAACTTTTCTCATCTTGGAGTGTTCTGTTACTTAGAAAATCATACTTTTGGTCGCCTCGACTTTGTCACTTTTGATACTCATTTTCTCTTCCAATGCAA

mRNA sequence

ATGAATTCTCCATTTTTGGAGCTAACCGCAAGCATGTTCGCTTTTGCCAAAGCTCAGAAGACGAAGGCCTACTTCAAACGTTATCAAGTCAAGTTCAAGAGAAGGAGAGAGGGAAAGACTGACTACCGAGCTAGAATTCGTCTTATTAACCAGGACAAGAACAAGTACAACACTCCCAAGTATCGCATTGTTGTCCGATTTTCGAACAAAGATATCACAGCTCAGATAATTTCTGCTAGCATTGCTGGGGACTTGGTCCTTGCTTCAGCTTATTCTCATGAGCTTCCTCGTTACGGGCTGGAAGTTGGTCTCACCAACTATGCTGCAGCATATTGCACTGGGCTTCTCTTGGCTCGACGAGTTTTGAAACAGCTTGAGATGGATGATGAATATGAGGGCAATGTGGAGGCTACTGGTGAAGATTACTCTGTTGAGCCTGCTGATACCAGAAGGCCATTCCGTGCTTTACTTGATGTCGGTCTTCTTAAAACTACAACTGGAAACCGTGTTTTTGGTGCTCTTAAGGGAGCTTTAGATGGTGGATTGGACATCCCTCACAGCGATAAGAGATTTGCTGGGTTTTCCAAGGATAGCAAGCAGCTTGATGCTGATGTTCATAGGAAGTACATTTATGGTGGACACGTTGCGGCGTACATGCGGACCTTGATGGAAGATGAACCAGAGAAGTACCAAACCCACTTCAGCGAATACATCAAGAAGGGCATTGAGGCTGATGACATCGAGGGTTTATACAAGAAAGTTCATGCCGCCATTCGGGCAGACCCATCAGTGAAGAAATCCGACAAGCCCCAACCCAAGGCACACAAGAGGTACAACTTGAAGAAGCTGACATACGACGAAAGAAAAGCTCGCTTGGTGGAACGTTTGAACGCTCTCAACTCTGCAGCTAATGCTGACGACGACGATGACGATGAGGATGACGAGTGAAAGATGAAAATTAGTTGATGTGGGCATGGAAGGATTTTAATGTAGTAGTTCTGCTATTTATTATTTTAAGTTTCGTTCTTTGGTCGAAAACTTTTCTCATCTTGGAGTGTTCTGTTACTTAGAAAATCATACTTTTGGTCGCCTCGACTTTGTCACTTTTGATACTCATTTTCTCTTCCAATGCAA

Coding sequence (CDS)

ATGAATTCTCCATTTTTGGAGCTAACCGCAAGCATGTTCGCTTTTGCCAAAGCTCAGAAGACGAAGGCCTACTTCAAACGTTATCAAGTCAAGTTCAAGAGAAGGAGAGAGGGAAAGACTGACTACCGAGCTAGAATTCGTCTTATTAACCAGGACAAGAACAAGTACAACACTCCCAAGTATCGCATTGTTGTCCGATTTTCGAACAAAGATATCACAGCTCAGATAATTTCTGCTAGCATTGCTGGGGACTTGGTCCTTGCTTCAGCTTATTCTCATGAGCTTCCTCGTTACGGGCTGGAAGTTGGTCTCACCAACTATGCTGCAGCATATTGCACTGGGCTTCTCTTGGCTCGACGAGTTTTGAAACAGCTTGAGATGGATGATGAATATGAGGGCAATGTGGAGGCTACTGGTGAAGATTACTCTGTTGAGCCTGCTGATACCAGAAGGCCATTCCGTGCTTTACTTGATGTCGGTCTTCTTAAAACTACAACTGGAAACCGTGTTTTTGGTGCTCTTAAGGGAGCTTTAGATGGTGGATTGGACATCCCTCACAGCGATAAGAGATTTGCTGGGTTTTCCAAGGATAGCAAGCAGCTTGATGCTGATGTTCATAGGAAGTACATTTATGGTGGACACGTTGCGGCGTACATGCGGACCTTGATGGAAGATGAACCAGAGAAGTACCAAACCCACTTCAGCGAATACATCAAGAAGGGCATTGAGGCTGATGACATCGAGGGTTTATACAAGAAAGTTCATGCCGCCATTCGGGCAGACCCATCAGTGAAGAAATCCGACAAGCCCCAACCCAAGGCACACAAGAGGTACAACTTGAAGAAGCTGACATACGACGAAAGAAAAGCTCGCTTGGTGGAACGTTTGAACGCTCTCAACTCTGCAGCTAATGCTGACGACGACGATGACGATGAGGATGACGAGTGA

Protein sequence

MNSPFLELTASMFAFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDITAQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEGNVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAGFSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKKVHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDEDDE
Homology
BLAST of Carg21212-RA vs. NCBI nr
Match: KAG7012684.1 (60S ribosomal protein L5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 611.7 bits (1576), Expect = 3.6e-171
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MNSPFLELTASMFAFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPK 60
           MNSPFLELTASMFAFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPK
Sbjct: 1   MNSPFLELTASMFAFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPK 60

Query: 61  YRIVVRFSNKDITAQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARR 120
           YRIVVRFSNKDITAQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARR
Sbjct: 61  YRIVVRFSNKDITAQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARR 120

Query: 121 VLKQLEMDDEYEGNVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDG 180
           VLKQLEMDDEYEGNVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDG
Sbjct: 121 VLKQLEMDDEYEGNVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDG 180

Query: 181 GLDIPHSDKRFAGFSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKK 240
           GLDIPHSDKRFAGFSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKK
Sbjct: 181 GLDIPHSDKRFAGFSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKK 240

Query: 241 GIEADDIEGLYKKVHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALN 300
           GIEADDIEGLYKKVHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALN
Sbjct: 241 GIEADDIEGLYKKVHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALN 300

Query: 301 SAANADDDDDDEDDE 316
           SAANADDDDDDEDDE
Sbjct: 301 SAANADDDDDDEDDE 315

BLAST of Carg21212-RA vs. NCBI nr
Match: XP_023541888.1 (60S ribosomal protein L5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 584.7 bits (1506), Expect = 4.7e-163
Identity = 301/302 (99.67%), Postives = 301/302 (99.67%), Query Frame = 0

Query: 14  AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 73
           AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT
Sbjct: 2   AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 61

Query: 74  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 133
           AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG
Sbjct: 62  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 121

Query: 134 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 193
           NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG
Sbjct: 122 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 181

Query: 194 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 253
           FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK
Sbjct: 182 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 241

Query: 254 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 313
           VHAAIRADP VKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED
Sbjct: 242 VHAAIRADPLVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 301

Query: 314 DE 316
           DE
Sbjct: 302 DE 303

BLAST of Carg21212-RA vs. NCBI nr
Match: KAG6573600.1 (60S ribosomal protein L5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 584.3 bits (1505), Expect = 6.1e-163
Identity = 300/302 (99.34%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 14  AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 73
           AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT
Sbjct: 2   AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 61

Query: 74  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 133
           AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG
Sbjct: 62  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 121

Query: 134 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 193
           NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG
Sbjct: 122 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 181

Query: 194 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 253
           FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK
Sbjct: 182 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 241

Query: 254 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 313
           VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED
Sbjct: 242 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 301

Query: 314 DE 316
           ++
Sbjct: 302 ED 303

BLAST of Carg21212-RA vs. NCBI nr
Match: XP_022945687.1 (60S ribosomal protein L5-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 583.2 bits (1502), Expect = 1.4e-162
Identity = 299/302 (99.01%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 14  AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 73
           AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT
Sbjct: 2   AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 61

Query: 74  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 133
           AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG
Sbjct: 62  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 121

Query: 134 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 193
           NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG
Sbjct: 122 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 181

Query: 194 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 253
           FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK
Sbjct: 182 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 241

Query: 254 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 313
           VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDD+D
Sbjct: 242 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDDD 301

Query: 314 DE 316
           ++
Sbjct: 302 ED 303

BLAST of Carg21212-RA vs. NCBI nr
Match: XP_022966860.1 (60S ribosomal protein L5-like [Cucurbita maxima])

HSP 1 Score: 578.2 bits (1489), Expect = 4.4e-161
Identity = 297/302 (98.34%), Postives = 300/302 (99.34%), Query Frame = 0

Query: 14  AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 73
           AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT
Sbjct: 2   AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 61

Query: 74  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 133
           AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG
Sbjct: 62  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 121

Query: 134 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 193
           NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG
Sbjct: 122 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 181

Query: 194 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 253
           FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEAD IEGLYKK
Sbjct: 182 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADGIEGLYKK 241

Query: 254 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 313
           VHAAIRADPSVKKSDKP PKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDD++
Sbjct: 242 VHAAIRADPSVKKSDKPPPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDDE 301

Query: 314 DE 316
           D+
Sbjct: 302 DD 303

BLAST of Carg21212-RA vs. ExPASy Swiss-Prot
Match: Q6UNT2 (60S ribosomal protein L5 OS=Cucumis sativus OX=3659 GN=RPL5 PE=2 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 2.1e-153
Identity = 275/301 (91.36%), Postives = 292/301 (97.01%), Query Frame = 0

Query: 14  AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 73
           AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYR VVR SNKDIT
Sbjct: 2   AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRFVVRTSNKDIT 61

Query: 74  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 133
           AQIISASIAGDLVLASAYSHELP+YGLEVGLTNYAAAYCTGLLLARRVLK LEMD EYEG
Sbjct: 62  AQIISASIAGDLVLASAYSHELPQYGLEVGLTNYAAAYCTGLLLARRVLKMLEMDAEYEG 121

Query: 134 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 193
           NVEATGEDYSVEPADTRRPFRALLDVGL++TTTGNRVFGALKGALDGGLDIPHSDKRFAG
Sbjct: 122 NVEATGEDYSVEPADTRRPFRALLDVGLIRTTTGNRVFGALKGALDGGLDIPHSDKRFAG 181

Query: 194 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 253
           ++K+ +QLD +VHRKYI+GGHVAAYMRTLMEDEPEKYQ+HFSEYIKKGIEAD++EGLYKK
Sbjct: 182 YAKNGQQLDVEVHRKYIFGGHVAAYMRTLMEDEPEKYQSHFSEYIKKGIEADELEGLYKK 241

Query: 254 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 313
           VHAAIRA+P  KKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAA+ DDDDD++D
Sbjct: 242 VHAAIRANPIAKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAADGDDDDDEDD 301

Query: 314 D 315
           +
Sbjct: 302 E 302

BLAST of Carg21212-RA vs. ExPASy Swiss-Prot
Match: A2WXX3 (60S ribosomal protein L5-1 OS=Oryza sativa subsp. indica OX=39946 GN=RPL5A PE=3 SV=2)

HSP 1 Score: 496.1 bits (1276), Expect = 2.9e-139
Identity = 246/304 (80.92%), Postives = 276/304 (90.79%), Query Frame = 0

Query: 12  MFAFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKD 71
           M  F K QKT AY+KR+QVKFKRRR+GKTDYRARIRL NQDKNKYNTPKYR VVRF+NKD
Sbjct: 1   MGGFVKTQKTNAYYKRFQVKFKRRRQGKTDYRARIRLTNQDKNKYNTPKYRFVVRFTNKD 60

Query: 72  ITAQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEY 131
           ITAQI+ A+IAGD+V+A+AYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLK   +D EY
Sbjct: 61  ITAQIVYATIAGDIVMAAAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKLRGLDQEY 120

Query: 132 EGNVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRF 191
           EGN+EATGEDY VEPAD RRPFRALLDVGL++TTTGNRVFGALKGALDGGLDIPHSDKRF
Sbjct: 121 EGNIEATGEDYYVEPADERRPFRALLDVGLIRTTTGNRVFGALKGALDGGLDIPHSDKRF 180

Query: 192 AGFSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLY 251
           AGF KD KQLD+D+HRKYIYGGHVA YMR++ E+EPEK+Q HFSEY+KKGI+AD +E LY
Sbjct: 181 AGFKKDEKQLDSDIHRKYIYGGHVADYMRSMAEEEPEKFQAHFSEYLKKGIDADGMEALY 240

Query: 252 KKVHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDD 311
           KKVHAAIRADP++ KS K +P  HKRYNLKKLTY++RKA LVERLNALNS+A ADDDD++
Sbjct: 241 KKVHAAIRADPTMAKSTKKEPATHKRYNLKKLTYEQRKASLVERLNALNSSAGADDDDEE 300

Query: 312 EDDE 316
           EDDE
Sbjct: 301 EDDE 304

BLAST of Carg21212-RA vs. ExPASy Swiss-Prot
Match: Q8L4L4 (60S ribosomal protein L5-2 OS=Oryza sativa subsp. japonica OX=39947 GN=RPL5B PE=2 SV=2)

HSP 1 Score: 495.0 bits (1273), Expect = 6.4e-139
Identity = 247/304 (81.25%), Postives = 275/304 (90.46%), Query Frame = 0

Query: 12  MFAFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKD 71
           M  F K QKT AYFKR+QVKFKRRR+GKTDYRARIRL NQDKNKYNTPKYR VVRF+NKD
Sbjct: 1   MGGFVKTQKTHAYFKRFQVKFKRRRQGKTDYRARIRLTNQDKNKYNTPKYRFVVRFTNKD 60

Query: 72  ITAQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEY 131
           ITAQI+ A+IAGD+V+A+AYSHELPRYGLEVGLTNYAAAYCTGLLLARRVL    +D EY
Sbjct: 61  ITAQIVYATIAGDIVMAAAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLTLRGLDQEY 120

Query: 132 EGNVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRF 191
           EGNVEATGEDY VEPAD RRPFRALLDVGL++TTTGNRVFGALKGALDGGLDIPHSDKRF
Sbjct: 121 EGNVEATGEDYYVEPADERRPFRALLDVGLIRTTTGNRVFGALKGALDGGLDIPHSDKRF 180

Query: 192 AGFSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLY 251
           AGF KD KQLD+D+HRKYIYGGHVA YMR++ E+EPEK+Q HFSEY+KKGI+AD +E LY
Sbjct: 181 AGFKKDEKQLDSDIHRKYIYGGHVADYMRSMAEEEPEKFQAHFSEYLKKGIDADGMESLY 240

Query: 252 KKVHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDD 311
           KKVHAAIRADP++ KS K +P  HKRYNLKKLTY++RKA LVERLNALNS+A ADDDD++
Sbjct: 241 KKVHAAIRADPTMAKSTKKEPATHKRYNLKKLTYEQRKASLVERLNALNSSAGADDDDEE 300

Query: 312 EDDE 316
           EDDE
Sbjct: 301 EDDE 304

BLAST of Carg21212-RA vs. ExPASy Swiss-Prot
Match: Q0JGY1 (60S ribosomal protein L5-1 OS=Oryza sativa subsp. japonica OX=39947 GN=RPL5A PE=2 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 8.4e-139
Identity = 246/304 (80.92%), Postives = 275/304 (90.46%), Query Frame = 0

Query: 12  MFAFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKD 71
           M  F K QKT AY KR+QVKFKRRR+GKTDYRARIRL NQDKNKYNTPKYR VVRF+NKD
Sbjct: 1   MGGFVKTQKTNAYHKRFQVKFKRRRQGKTDYRARIRLTNQDKNKYNTPKYRFVVRFTNKD 60

Query: 72  ITAQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEY 131
           ITAQI+ A+IAGD+V+A+AYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLK   +D EY
Sbjct: 61  ITAQIVYATIAGDIVMAAAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKLRGLDQEY 120

Query: 132 EGNVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRF 191
           EGN+EATGEDY VEPAD RRPFRALLDVGL++TTTGNRVFGALKGALDGGLDIPHSDKRF
Sbjct: 121 EGNIEATGEDYYVEPADERRPFRALLDVGLIRTTTGNRVFGALKGALDGGLDIPHSDKRF 180

Query: 192 AGFSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLY 251
           AGF KD KQLD+D+HRKYIYGGHVA YMR++ E+EPEK+Q HFSEY+KKGI+AD +E LY
Sbjct: 181 AGFKKDEKQLDSDIHRKYIYGGHVADYMRSMAEEEPEKFQAHFSEYLKKGIDADGMEALY 240

Query: 252 KKVHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDD 311
           KKVHAAIRADP++ KS K +P  HKRYNLKKLTY++RKA LVERLNALNS+A ADDDD++
Sbjct: 241 KKVHAAIRADPTMAKSTKKEPATHKRYNLKKLTYEQRKASLVERLNALNSSAGADDDDEE 300

Query: 312 EDDE 316
           EDDE
Sbjct: 301 EDDE 304

BLAST of Carg21212-RA vs. ExPASy Swiss-Prot
Match: Q8LBI1 (60S ribosomal protein L5-1 OS=Arabidopsis thaliana OX=3702 GN=RPL5A PE=1 SV=2)

HSP 1 Score: 487.3 bits (1253), Expect = 1.3e-136
Identity = 242/299 (80.94%), Postives = 271/299 (90.64%), Query Frame = 0

Query: 15  FAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDITA 74
           F K+ K+ AYFKRYQVKF+RRR+GKTDYRARIRLINQDKNKYNTPKYR VVRF+NKDI A
Sbjct: 3   FVKSTKSNAYFKRYQVKFRRRRDGKTDYRARIRLINQDKNKYNTPKYRFVVRFTNKDIVA 62

Query: 75  QIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEGN 134
           QI+SASIAGD+V ASAY+HELP+YGL VGLTNYAAAYCTGLLLARRVLK LEMDDEYEGN
Sbjct: 63  QIVSASIAGDIVKASAYAHELPQYGLTVGLTNYAAAYCTGLLLARRVLKMLEMDDEYEGN 122

Query: 135 VEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAGF 194
           VEATGED+SVEP D+RRPFRALLDVGL++TTTGNRVFGALKGALDGGLDIPHSDKRFAGF
Sbjct: 123 VEATGEDFSVEPTDSRRPFRALLDVGLIRTTTGNRVFGALKGALDGGLDIPHSDKRFAGF 182

Query: 195 SKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKKV 254
            K++KQLDA++HR YIYGGHV+ YM+ L EDEPEK QTHFS YIKKG+EA+ IE LYKKV
Sbjct: 183 HKENKQLDAEIHRNYIYGGHVSNYMKLLGEDEPEKLQTHFSAYIKKGVEAESIEELYKKV 242

Query: 255 HAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 314
           HAAIRADP+ KK+ KP PK HKRYNLKKLTY+ERK +L+ER+ ALN A   DDD+DDE+
Sbjct: 243 HAAIRADPNPKKTVKPAPKQHKRYNLKKLTYEERKNKLIERVKALNGAGGDDDDEDDEE 301

BLAST of Carg21212-RA vs. ExPASy TrEMBL
Match: A0A6J1G1M2 (60S ribosomal protein L5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449844 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.6e-163
Identity = 299/302 (99.01%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 14  AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 73
           AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT
Sbjct: 2   AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 61

Query: 74  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 133
           AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG
Sbjct: 62  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 121

Query: 134 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 193
           NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG
Sbjct: 122 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 181

Query: 194 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 253
           FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK
Sbjct: 182 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 241

Query: 254 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 313
           VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDD+D
Sbjct: 242 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDDD 301

Query: 314 DE 316
           ++
Sbjct: 302 ED 303

BLAST of Carg21212-RA vs. ExPASy TrEMBL
Match: A0A6J1HSR8 (60S ribosomal protein L5-like OS=Cucurbita maxima OX=3661 GN=LOC111466439 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.1e-161
Identity = 297/302 (98.34%), Postives = 300/302 (99.34%), Query Frame = 0

Query: 14  AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 73
           AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT
Sbjct: 2   AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 61

Query: 74  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 133
           AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG
Sbjct: 62  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 121

Query: 134 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 193
           NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG
Sbjct: 122 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 181

Query: 194 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 253
           FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEAD IEGLYKK
Sbjct: 182 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADGIEGLYKK 241

Query: 254 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 313
           VHAAIRADPSVKKSDKP PKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDD++
Sbjct: 242 VHAAIRADPSVKKSDKPPPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDDE 301

Query: 314 DE 316
           D+
Sbjct: 302 DD 303

BLAST of Carg21212-RA vs. ExPASy TrEMBL
Match: A0A6J1G1K9 (60S ribosomal protein L5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449844 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 8.4e-158
Identity = 299/335 (89.25%), Postives = 302/335 (90.15%), Query Frame = 0

Query: 14  AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 73
           AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT
Sbjct: 2   AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 61

Query: 74  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 133
           AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG
Sbjct: 62  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 121

Query: 134 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 193
           NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG
Sbjct: 122 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 181

Query: 194 FSKDSKQLDADVHRKYIYGGHVAAYMR--------------------------------- 253
           FSKDSKQLDADVHRKYIYGGHVAAYMR                                 
Sbjct: 182 FSKDSKQLDADVHRKYIYGGHVAAYMRVRPCCLFVLMTCYLYPSKKLYKRLVTFYIWLHQ 241

Query: 254 TLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKKVHAAIRADPSVKKSDKPQPKAHKRYNL 313
           TLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKKVHAAIRADPSVKKSDKPQPKAHKRYNL
Sbjct: 242 TLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKKVHAAIRADPSVKKSDKPQPKAHKRYNL 301

Query: 314 KKLTYDERKARLVERLNALNSAANADDDDDDEDDE 316
           KKLTYDERKARLVERLNALNSAANADDDDDD+D++
Sbjct: 302 KKLTYDERKARLVERLNALNSAANADDDDDDDDED 336

BLAST of Carg21212-RA vs. ExPASy TrEMBL
Match: A0A6J1GXQ9 (60S ribosomal protein L5 OS=Cucurbita moschata OX=3662 GN=LOC111458161 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.6e-156
Identity = 289/303 (95.38%), Postives = 297/303 (98.02%), Query Frame = 0

Query: 14  AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 73
           AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYR VVRFSNKD+T
Sbjct: 2   AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRFVVRFSNKDVT 61

Query: 74  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 133
           AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLK LEMD+EYEG
Sbjct: 62  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKMLEMDEEYEG 121

Query: 134 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 193
           NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHS+KRFAG
Sbjct: 122 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSEKRFAG 181

Query: 194 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 253
           FSKD KQLDA+VHRKYI+GGHVAAYMRTLMEDEPEKYQ+HFS+YIKKGIEAD+IEGLYKK
Sbjct: 182 FSKDGKQLDAEVHRKYIFGGHVAAYMRTLMEDEPEKYQSHFSQYIKKGIEADNIEGLYKK 241

Query: 254 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANAD-DDDDDE 313
           VHAAIRADP VKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNS ANAD DDDDDE
Sbjct: 242 VHAAIRADPVVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSKANADEDDDDDE 301

Query: 314 DDE 316
           DDE
Sbjct: 302 DDE 304

BLAST of Carg21212-RA vs. ExPASy TrEMBL
Match: A0A6J1KC97 (60S ribosomal protein L5 OS=Cucurbita maxima OX=3661 GN=LOC111493018 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.3e-155
Identity = 287/303 (94.72%), Postives = 297/303 (98.02%), Query Frame = 0

Query: 14  AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDIT 73
           AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYR VVRFSNKD+T
Sbjct: 2   AFAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRFVVRFSNKDVT 61

Query: 74  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEG 133
           AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLK L+MD+EYEG
Sbjct: 62  AQIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKMLDMDEEYEG 121

Query: 134 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAG 193
           NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHS+KRFAG
Sbjct: 122 NVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSEKRFAG 181

Query: 194 FSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKK 253
           FSKD KQLDA+VHRKYI+GGHVAAYMRTLMEDEPEKYQ+HFS+YIKKGIEAD+IEGLYKK
Sbjct: 182 FSKDGKQLDAEVHRKYIFGGHVAAYMRTLMEDEPEKYQSHFSQYIKKGIEADNIEGLYKK 241

Query: 254 VHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANAD-DDDDDE 313
           VHAAIRADP VKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNS A+AD DDDDDE
Sbjct: 242 VHAAIRADPVVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSKAHADEDDDDDE 301

Query: 314 DDE 316
           DDE
Sbjct: 302 DDE 304

BLAST of Carg21212-RA vs. TAIR 10
Match: AT3G25520.1 (ribosomal protein L5 )

HSP 1 Score: 487.3 bits (1253), Expect = 9.5e-138
Identity = 242/299 (80.94%), Postives = 271/299 (90.64%), Query Frame = 0

Query: 15  FAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDITA 74
           F K+ K+ AYFKRYQVKF+RRR+GKTDYRARIRLINQDKNKYNTPKYR VVRF+NKDI A
Sbjct: 3   FVKSTKSNAYFKRYQVKFRRRRDGKTDYRARIRLINQDKNKYNTPKYRFVVRFTNKDIVA 62

Query: 75  QIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEGN 134
           QI+SASIAGD+V ASAY+HELP+YGL VGLTNYAAAYCTGLLLARRVLK LEMDDEYEGN
Sbjct: 63  QIVSASIAGDIVKASAYAHELPQYGLTVGLTNYAAAYCTGLLLARRVLKMLEMDDEYEGN 122

Query: 135 VEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAGF 194
           VEATGED+SVEP D+RRPFRALLDVGL++TTTGNRVFGALKGALDGGLDIPHSDKRFAGF
Sbjct: 123 VEATGEDFSVEPTDSRRPFRALLDVGLIRTTTGNRVFGALKGALDGGLDIPHSDKRFAGF 182

Query: 195 SKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKKV 254
            K++KQLDA++HR YIYGGHV+ YM+ L EDEPEK QTHFS YIKKG+EA+ IE LYKKV
Sbjct: 183 HKENKQLDAEIHRNYIYGGHVSNYMKLLGEDEPEKLQTHFSAYIKKGVEAESIEELYKKV 242

Query: 255 HAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 314
           HAAIRADP+ KK+ KP PK HKRYNLKKLTY+ERK +L+ER+ ALN A   DDD+DDE+
Sbjct: 243 HAAIRADPNPKKTVKPAPKQHKRYNLKKLTYEERKNKLIERVKALNGAGGDDDDEDDEE 301

BLAST of Carg21212-RA vs. TAIR 10
Match: AT5G39740.1 (ribosomal protein L5 B )

HSP 1 Score: 483.8 bits (1244), Expect = 1.1e-136
Identity = 239/299 (79.93%), Postives = 271/299 (90.64%), Query Frame = 0

Query: 15  FAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDITA 74
           F K+ K+ AYFKRYQVKF+RRR+GKTDYRARIRLINQDKNKYNTPKYR VVRF+NKDI A
Sbjct: 3   FVKSSKSNAYFKRYQVKFRRRRDGKTDYRARIRLINQDKNKYNTPKYRFVVRFTNKDIVA 62

Query: 75  QIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEGN 134
           QI+SASIAGD+V ASAY+HELP+YGL VGLTNYAAAYCTGLLLARRVLK LEMDDEYEGN
Sbjct: 63  QIVSASIAGDIVKASAYAHELPQYGLTVGLTNYAAAYCTGLLLARRVLKMLEMDDEYEGN 122

Query: 135 VEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAGF 194
           VEATGED+SVEP D+RRPFRALLDVGL++TTTGNRVFGALKGALDGGLDIPHSDKRFAGF
Sbjct: 123 VEATGEDFSVEPTDSRRPFRALLDVGLIRTTTGNRVFGALKGALDGGLDIPHSDKRFAGF 182

Query: 195 SKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKKV 254
            K++KQLDA++HR YIYGGHV+ YM+ L EDEPEK QTHFS YIKKG+EA+ IE +YKKV
Sbjct: 183 HKENKQLDAEIHRNYIYGGHVSNYMKLLGEDEPEKLQTHFSAYIKKGVEAESIEEMYKKV 242

Query: 255 HAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 314
           HAAIRA+P+ KK++K  PK HKRYNLKKLTY+ERK +L+ER+ ALN A   DDD+DDE+
Sbjct: 243 HAAIRAEPNHKKTEKSAPKEHKRYNLKKLTYEERKNKLIERVKALNGAGGDDDDEDDEE 301

BLAST of Carg21212-RA vs. TAIR 10
Match: AT5G39740.2 (ribosomal protein L5 B )

HSP 1 Score: 483.8 bits (1244), Expect = 1.1e-136
Identity = 239/299 (79.93%), Postives = 271/299 (90.64%), Query Frame = 0

Query: 15  FAKAQKTKAYFKRYQVKFKRRREGKTDYRARIRLINQDKNKYNTPKYRIVVRFSNKDITA 74
           F K+ K+ AYFKRYQVKF+RRR+GKTDYRARIRLINQDKNKYNTPKYR VVRF+NKDI A
Sbjct: 3   FVKSSKSNAYFKRYQVKFRRRRDGKTDYRARIRLINQDKNKYNTPKYRFVVRFTNKDIVA 62

Query: 75  QIISASIAGDLVLASAYSHELPRYGLEVGLTNYAAAYCTGLLLARRVLKQLEMDDEYEGN 134
           QI+SASIAGD+V ASAY+HELP+YGL VGLTNYAAAYCTGLLLARRVLK LEMDDEYEGN
Sbjct: 63  QIVSASIAGDIVKASAYAHELPQYGLTVGLTNYAAAYCTGLLLARRVLKMLEMDDEYEGN 122

Query: 135 VEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDIPHSDKRFAGF 194
           VEATGED+SVEP D+RRPFRALLDVGL++TTTGNRVFGALKGALDGGLDIPHSDKRFAGF
Sbjct: 123 VEATGEDFSVEPTDSRRPFRALLDVGLIRTTTGNRVFGALKGALDGGLDIPHSDKRFAGF 182

Query: 195 SKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEADDIEGLYKKV 254
            K++KQLDA++HR YIYGGHV+ YM+ L EDEPEK QTHFS YIKKG+EA+ IE +YKKV
Sbjct: 183 HKENKQLDAEIHRNYIYGGHVSNYMKLLGEDEPEKLQTHFSAYIKKGVEAESIEEMYKKV 242

Query: 255 HAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAANADDDDDDED 314
           HAAIRA+P+ KK++K  PK HKRYNLKKLTY+ERK +L+ER+ ALN A   DDD+DDE+
Sbjct: 243 HAAIRAEPNHKKTEKSAPKEHKRYNLKKLTYEERKNKLIERVKALNGAGGDDDDEDDEE 301

BLAST of Carg21212-RA vs. TAIR 10
Match: AT3G25520.2 (ribosomal protein L5 )

HSP 1 Score: 305.8 bits (782), Expect = 3.9e-83
Identity = 149/189 (78.84%), Postives = 169/189 (89.42%), Query Frame = 0

Query: 125 LEMDDEYEGNVEATGEDYSVEPADTRRPFRALLDVGLLKTTTGNRVFGALKGALDGGLDI 184
           LEMDDEYEGNVEATGED+SVEP D+RRPFRALLDVGL++TTTGNRVFGALKGALDGGLDI
Sbjct: 2   LEMDDEYEGNVEATGEDFSVEPTDSRRPFRALLDVGLIRTTTGNRVFGALKGALDGGLDI 61

Query: 185 PHSDKRFAGFSKDSKQLDADVHRKYIYGGHVAAYMRTLMEDEPEKYQTHFSEYIKKGIEA 244
           PHSDKRFAGF K++KQLDA++HR YIYGGHV+ YM+ L EDEPEK QTHFS YIKKG+EA
Sbjct: 62  PHSDKRFAGFHKENKQLDAEIHRNYIYGGHVSNYMKLLGEDEPEKLQTHFSAYIKKGVEA 121

Query: 245 DDIEGLYKKVHAAIRADPSVKKSDKPQPKAHKRYNLKKLTYDERKARLVERLNALNSAAN 304
           + IE LYKKVHAAIRADP+ KK+ KP PK HKRYNLKKLTY+ERK +L+ER+ ALN A  
Sbjct: 122 ESIEELYKKVHAAIRADPNPKKTVKPAPKQHKRYNLKKLTYEERKNKLIERVKALNGAGG 181

Query: 305 ADDDDDDED 314
            DDD+DDE+
Sbjct: 182 DDDDEDDEE 190

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012684.13.6e-171100.0060S ribosomal protein L5, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023541888.14.7e-16399.6760S ribosomal protein L5-like [Cucurbita pepo subsp. pepo][more]
KAG6573600.16.1e-16399.3460S ribosomal protein L5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022945687.11.4e-16299.0160S ribosomal protein L5-like isoform X2 [Cucurbita moschata][more]
XP_022966860.14.4e-16198.3460S ribosomal protein L5-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q6UNT22.1e-15391.3660S ribosomal protein L5 OS=Cucumis sativus OX=3659 GN=RPL5 PE=2 SV=1[more]
A2WXX32.9e-13980.9260S ribosomal protein L5-1 OS=Oryza sativa subsp. indica OX=39946 GN=RPL5A PE=3 ... [more]
Q8L4L46.4e-13981.2560S ribosomal protein L5-2 OS=Oryza sativa subsp. japonica OX=39947 GN=RPL5B PE=... [more]
Q0JGY18.4e-13980.9260S ribosomal protein L5-1 OS=Oryza sativa subsp. japonica OX=39947 GN=RPL5A PE=... [more]
Q8LBI11.3e-13680.9460S ribosomal protein L5-1 OS=Arabidopsis thaliana OX=3702 GN=RPL5A PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1G1M26.6e-16399.0160S ribosomal protein L5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1HSR82.1e-16198.3460S ribosomal protein L5-like OS=Cucurbita maxima OX=3661 GN=LOC111466439 PE=3 S... [more]
A0A6J1G1K98.4e-15889.2560S ribosomal protein L5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1GXQ91.6e-15695.3860S ribosomal protein L5 OS=Cucurbita moschata OX=3662 GN=LOC111458161 PE=3 SV=1[more]
A0A6J1KC971.3e-15594.7260S ribosomal protein L5 OS=Cucurbita maxima OX=3661 GN=LOC111493018 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G25520.19.5e-13880.94ribosomal protein L5 [more]
AT5G39740.11.1e-13679.93ribosomal protein L5 B [more]
AT5G39740.21.1e-13679.93ribosomal protein L5 B [more]
AT3G25520.23.9e-8378.84ribosomal protein L5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005485Ribosomal protein L5 eukaryotic/L18 archaealPRINTSPR00058RIBOSOMALL5coord: 55..74
score: 72.19
coord: 218..238
score: 49.4
coord: 32..51
score: 76.25
coord: 176..195
score: 70.0
coord: 96..116
score: 83.33
coord: 156..175
score: 72.19
IPR005485Ribosomal protein L5 eukaryotic/L18 archaealPFAMPF17144Ribosomal_L5ecoord: 26..187
e-value: 9.9E-82
score: 272.8
IPR005485Ribosomal protein L5 eukaryotic/L18 archaealPANTHERPTHR23410RIBOSOMAL PROTEIN L5-RELATEDcoord: 14..310
IPR005485Ribosomal protein L5 eukaryotic/L18 archaealHAMAPMF_01337_ARibosomal_L18_Acoord: 26..258
score: 22.124208
IPR025607Ribosomal protein L5 eukaryotic/L18 archaeal, C-terminalPFAMPF14204Ribosomal_L18_ccoord: 203..293
e-value: 3.7E-35
score: 120.7
NoneNo IPR availableGENE3D3.30.420.100coord: 13..303
e-value: 1.3E-145
score: 486.5
NoneNo IPR availablePANTHERPTHR23410:SF28SUBFAMILY NOT NAMEDcoord: 14..310
NoneNo IPR availableCDDcd00432Ribosomal_L18_L5ecoord: 53..182
e-value: 2.2962E-27
score: 101.077
NoneNo IPR availableSUPERFAMILY53137Translational machinery componentscoord: 23..263

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg21212Carg21212gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg21212-RA:9Carg21212-RA:9exon
Carg21212-RA:8Carg21212-RA:8exon
Carg21212-RA:7Carg21212-RA:7exon
Carg21212-RA:6Carg21212-RA:6exon
Carg21212-RA:5Carg21212-RA:5exon
Carg21212-RA:4Carg21212-RA:4exon
Carg21212-RA:3Carg21212-RA:3exon
Carg21212-RA:2Carg21212-RA:2exon
Carg21212-RA:1Carg21212-RA:1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg21212-RA:three_prime_utrCarg21212-RA:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg21212-RA:cdsCarg21212-RA:cds_9CDS
Carg21212-RA:cdsCarg21212-RA:cds_8CDS
Carg21212-RA:cdsCarg21212-RA:cds_7CDS
Carg21212-RA:cdsCarg21212-RA:cds_6CDS
Carg21212-RA:cdsCarg21212-RA:cds_5CDS
Carg21212-RA:cdsCarg21212-RA:cds_4CDS
Carg21212-RA:cdsCarg21212-RA:cds_3CDS
Carg21212-RA:cdsCarg21212-RA:cds_2CDS
Carg21212-RA:cdsCarg21212-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg21212-RACarg21212-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006412 translation
biological_process GO:0000027 ribosomal large subunit assembly
cellular_component GO:0005840 ribosome
cellular_component GO:0022625 cytosolic large ribosomal subunit
cellular_component GO:0005634 nucleus
molecular_function GO:0008097 5S rRNA binding
molecular_function GO:0003735 structural constituent of ribosome