Carg20659-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20659-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	outer envelope pore protein 16, chloroplastic
Location	Carg_Chr13: 714846 .. 717220 (+)
Sequence length	1127
RNA-Seq Expression	Carg20659-RA
Synteny	Carg20659-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCAACGGTGAACAGAAGAACAATCCAAATTCAAGTACAAAATCACTCCCAACAAAATCAAATCATCTGGAGCGGCAAATTCCTTGTTCTGTAGCTTCAAGGATCCATTCAGCGCCATCCTCCACATTCAATCCCTTCGCTCTCACACAGTCTGTAGAACAAGACCATGCCTCGCACCGGCTTCGTCGGTTCCTTATCCACGCCCAAGGTCGACGTCGTCATCGACATGGGCAACCCCTTCCTCAACCTCACCGTTGATGGCTTCTTGAAGATCGGATCCGTATGTCTCTCTTTGTTTTTCTGAATTTTTCTTTCTGTTCTTCGTTTGCTCCGTCTATAATCTAAATGCGTTCTGTTCTGTTGCTTCTTTCTAGGTCGCGGCTACCCGAGCGGCTGCCGAGGATGCGTATCATGTCGTTAGAAAAGGTTTTGGTTGATGCGTATCATGTTGTTAGAAATTGGTTTCCTCCTTTCTGCAGTGATTTATGGCGTTGTTTTGTTTTTTCAGGAACGATTTCTAGTCACAATTTTGAAAACACGGTTAGTGTCTCTTGCTTCGTGTCTCAACTTGTGGAGTCTGGTTGTTTATGGCTTTTGATTGCTGAAATTTTCATTCTGAGAATCTTCATAAAGTGCGTAATTAGGAAACAGATAGATTCTTTATGGTCTTTTGATCTATTCGCCTAATACATCTTAGCTTCTATTACAGTTGAAGAAAATGTGTAAAGAAGGTGCATACTGGGGTAAGCATAGATCGCATTACTCCTCAATCAAGTACTTAGCTTAGTGGATGAGTTTCAGCATCTGTAAACCTCAGCCAATTTGATAAGATTTTTCTTTTGGTTCATTGTTCTTGGAAGTTAAGCTTATGAGATTAGAAAATCTGGCTATAATTTCAGAAACAATGGTGATATTTCATTTGAAAAGATGGGTAAAGGATTCTTTTCTGTTTGAACGCAGGAACCGTGGCCGGAGTTTACGTCGGTATGGAGTATGGGGTAGAAAGGATTCGCGGCAGAAGAGATTGGGTATGTTTTGCCATAAATTCAAACTCATCCTTTAAATCAAGTTCAAGTAATTCTGTTAGATTTTGCTTCCAGTTCTCTGTGTTTATTTGGGAATATTTTCATAGTTCTGTTTGATTTTCTGCCTCTCATCACTGTTCTGGATTGTTGGTTCACCTTGTAGAATGTATTGATCCTAGATTGGAGAAGAAAATTCAAACAACCCAGAAACAAAATTTCTCGAGATCCTAGATATATTATCAATGTTACTTACAATAGTCCTCTGATTAAAATGATTATATCTTTACAACAACAGAATCAATGTTTCTTTCCGGTTGTAGAACTGGGGCCTTAGTTGTCTGGATTATTTCAAACAAGAATTCCCCCCCCCCGAAGTTTGTAGTGTAACAGTCTAAGCCCATCACTAGCAGATATTGTAACGCCCTAGAACACGAGTGACGTCATTGATTATGTTATGTTGGGACTTGAAAGGGGGTTGAATGTGAGAGAACGAAGCATTCTCTATAAGGGTGTGGAAACCTCTCCCTAGCGAACGCCTTTTAAAAACTTTGAGGGGAATCTTGAAGGAAAAGTCCAAAGAGAACAATATCTGTTAGCAGTGGGCTTGGACGGTTGTCTGCTTGCTATGGTTTCTAATTGGGCATATCACCAATAGGTTGTTTGTCTTGGGATCTAAATGTTTGAGCTTGTTTCAGAAAAATGCAATGATTGGAGGTGCTTTGACGGGGGCTATGGTATCGGCAGCGAGCAACAGCAACCGGGACAAAATCGTTATCGATGCAATTACAGGAGGGGCTGTTGCGACTGCAGCAGAGTTCATAAACTACCTTACTTAAGCGCCGAGGTTTCCAACCACTTTCATGTCCCTTGAGAAGAAGGTATGGTGATTGAAGACGATGGAATCATGTTCAGTCTATTCAAACCTTTTCAGTTTTTTGCAGAATAACTAGTCTCTCGTAGAGTTGCAGCCTTAAAACATGGAAGAAAAAAGAGAAGATAGTGATAGAAATATCTTCTTTCTTCATCAATCAGAGCCACATGTGTTGTTGAGGCTGTAATCGTAGCTTTAGTTTAATGTCTTACGGAACAAATTTATTGCTTAAACTTTTAAGAAAGTGACGTTTCGTATGATGTTAATTTGTGGCCCTGGTTCTAATCTAGCAAATAAGTCTGTCATGAAAAATTTAGACTTCGTGGACGACGTCGATGTTTATATGACTTGGAATTGTTCATCAGCGACGTATATATAACTTGGGATTGTTCATCATCGACGTATATATAACTTGGGATTGTTCATCATCGACGTATATATAACTTGGGATTGTTTATCGTAAGTTCAAGTTATATATAA

mRNA sequence

TCAACGGTGAACAGAAGAACAATCCAAATTCAAGTACAAAATCACTCCCAACAAAATCAAATCATCTGGAGCGGCAAATTCCTTGTTCTGTAGCTTCAAGGATCCATTCAGCGCCATCCTCCACATTCAATCCCTTCGCTCTCACACAGTCTGTAGAACAAGACCATGCCTCGCACCGGCTTCGTCGGTTCCTTATCCACGCCCAAGGTCGACGTCGTCATCGACATGGGCAACCCCTTCCTCAACCTCACCGTTGATGGCTTCTTGAAGATCGGATCCGTCGCGGCTACCCGAGCGGCTGCCGAGGATGCGTATCATGTCGTTAGAAAAGGAACGATTTCTAGTCACAATTTTGAAAACACGGTTAGTGTCTCTTGCTTCGTGTCTCAACTTGTGGAGTCTGGAACCGTGGCCGGAGTTTACGTCGGTATGGAGTATGGGGTAGAAAGGATTCGCGGCAGAAGAGATTGGAAAAATGCAATGATTGGAGGTGCTTTGACGGGGGCTATGGTATCGGCAGCGAGCAACAGCAACCGGGACAAAATCGTTATCGATGCAATTACAGGAGGGGCTGTTGCGACTGCAGCAGAGTTCATAAACTACCTTACTTAAGCGCCGAGGTTTCCAACCACTTTCATGTCCCTTGAGAAGAAGGTATGGTGATTGAAGACGATGGAATCATGTTCAGTCTATTCAAACCTTTTCAGTTTTTTGCAGAATAACTAGTCTCTCGTAGAGTTGCAGCCTTAAAACATGGAAGAAAAAAGAGAAGATAGTGATAGAAATATCTTCTTTCTTCATCAATCAGAGCCACATGTGTTGTTGAGGCTGTAATCGTAGCTTTAGTTTAATGTCTTACGGAACAAATTTATTGCTTAAACTTTTAAGAAAGTGACGTTTCGTATGATGTTAATTTGTGGCCCTGGTTCTAATCTAGCAAATAAGTCTGTCATGAAAAATTTAGACTTCGTGGACGACGTCGATGTTTATATGACTTGGAATTGTTCATCAGCGACGTATATATAACTTGGGATTGTTCATCATCGACGTATATATAACTTGGGATTGTTCATCATCGACGTATATATAACTTGGGATTGTTTATCGTAAGTTCAAGTTATATATAA

Coding sequence (CDS)

ATGCCTCGCACCGGCTTCGTCGGTTCCTTATCCACGCCCAAGGTCGACGTCGTCATCGACATGGGCAACCCCTTCCTCAACCTCACCGTTGATGGCTTCTTGAAGATCGGATCCGTCGCGGCTACCCGAGCGGCTGCCGAGGATGCGTATCATGTCGTTAGAAAAGGAACGATTTCTAGTCACAATTTTGAAAACACGGTTAGTGTCTCTTGCTTCGTGTCTCAACTTGTGGAGTCTGGAACCGTGGCCGGAGTTTACGTCGGTATGGAGTATGGGGTAGAAAGGATTCGCGGCAGAAGAGATTGGAAAAATGCAATGATTGGAGGTGCTTTGACGGGGGCTATGGTATCGGCAGCGAGCAACAGCAACCGGGACAAAATCGTTATCGATGCAATTACAGGAGGGGCTGTTGCGACTGCAGCAGAGTTCATAAACTACCTTACTTAA

Protein sequence

MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISSHNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAASNSNRDKIVIDAITGGAVATAAEFINYLT

Homology

[Close]

BLAST of Carg20659-RA vs. NCBI nr
Match: KAG7019193.1 (Outer envelope pore protein 16, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 288.5 bits (737), Expect = 3.3e-74
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS
Sbjct: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS
Sbjct: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           NSNRDKIVIDAITGGAVATAAEFINYLT
Sbjct: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 148

[Close]

BLAST of Carg20659-RA vs. NCBI nr
Match: XP_022964801.1 (outer envelope pore protein 16, chloroplastic [Cucurbita moschata] >XP_022970582.1 outer envelope pore protein 16, chloroplastic [Cucurbita maxima] >XP_023519566.1 outer envelope pore protein 16, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6583432.1 Outer envelope pore protein 16, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 260.4 bits (664), Expect = 9.5e-66
Identity = 136/148 (91.89%), Postives = 138/148 (93.24%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS
Sbjct: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           HNFENT+   C   +    GTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS
Sbjct: 61  HNFENTLKKMC--KEGAYWGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           NSNRDKIVIDAITGGAVATAAEFINYLT
Sbjct: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 146

[Close]

BLAST of Carg20659-RA vs. NCBI nr
Match: KAA0067730.1 (outer envelope pore protein 16 [Cucumis melo var. makuwa])

HSP 1 Score: 258.8 bits (660), Expect = 2.8e-65
Identity = 133/148 (89.86%), Postives = 140/148 (94.59%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIS+
Sbjct: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIST 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           HNFENTVS+SCF  +L  +  VAG YVGMEYGVERIRG RDWKNAMIGGALTGA+VSAAS
Sbjct: 61  HNFENTVSISCFRLKLWRA--VAGAYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           N+NRDK+VIDAITGGAVATAAEFINYLT
Sbjct: 121 NNNRDKVVIDAITGGAVATAAEFINYLT 146

[Close]

BLAST of Carg20659-RA vs. NCBI nr
Match: XP_038893818.1 (outer envelope pore protein 16, chloroplastic [Benincasa hispida])

HSP 1 Score: 253.8 bits (647), Expect = 8.9e-64
Identity = 130/148 (87.84%), Postives = 137/148 (92.57%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPRTGF+GSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIS+
Sbjct: 1   MPRTGFIGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIST 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           HNFENT+   C   +    GTVAGVYVGMEYGVER+RG RDWKNAMIGGALTGA+VSAAS
Sbjct: 61  HNFENTLKKMC--KEGAYWGTVAGVYVGMEYGVERVRGTRDWKNAMIGGALTGALVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           N+NRDKIVIDAITGGAVATAAEFINYLT
Sbjct: 121 NNNRDKIVIDAITGGAVATAAEFINYLT 146

[Close]

BLAST of Carg20659-RA vs. NCBI nr
Match: XP_008457483.1 (PREDICTED: outer envelope pore protein 16, chloroplastic [Cucumis melo] >TYJ97413.1 outer envelope pore protein 16 [Cucumis melo var. makuwa])

HSP 1 Score: 250.8 bits (639), Expect = 7.6e-63
Identity = 128/148 (86.49%), Postives = 136/148 (91.89%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIS+
Sbjct: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIST 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           HNFENT+   C  ++    G VAG YVGMEYGVER+RG RDWKNAMIGGALTGA+VSAAS
Sbjct: 61  HNFENTLKKMC--TEGAYWGAVAGAYVGMEYGVERVRGTRDWKNAMIGGALTGALVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           N+NRDK+VIDAITGGAVATAAEFINYLT
Sbjct: 121 NNNRDKVVIDAITGGAVATAAEFINYLT 146

[Close]

BLAST of Carg20659-RA vs. ExPASy Swiss-Prot
Match: Q41050 (Outer envelope pore protein 16, chloroplastic OS=Pisum sativum OX=3888 GN=OEP16 PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.7e-55
Identity = 106/148 (71.62%), Postives = 127/148 (85.81%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPR+ F GSLS+PK+DVVIDMGNPFLNLTVDGFLKIG+VAATR+ AED +H++RKG+ISS
Sbjct: 1   MPRSSFSGSLSSPKLDVVIDMGNPFLNLTVDGFLKIGAVAATRSVAEDTFHIIRKGSISS 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           ++FE ++   C   +    G +AGVYVGMEYGVERIRG RDWKNAM GGA+TGA+VSAAS
Sbjct: 61  NDFEKSLKKMC--KEGAYWGAIAGVYVGMEYGVERIRGTRDWKNAMFGGAVTGALVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           N+ +DKI +DAITG A+ATAAEFINYLT
Sbjct: 121 NNKKDKIAVDAITGAAIATAAEFINYLT 146

[Close]

BLAST of Carg20659-RA vs. ExPASy Swiss-Prot
Match: Q9ZV24 (Outer envelope pore protein 16-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP161 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 5.5e-40
Identity = 80/145 (55.17%), Postives = 104/145 (71.72%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MP + F G++STPK+ V +DMGNPFLNLTVD FLKIG+V  T++ AED Y  + KG++S 
Sbjct: 1   MPSSTFSGTVSTPKLSVAVDMGNPFLNLTVDAFLKIGAVGVTKSLAEDTYKAIDKGSLSK 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
              E+ +   C   + V  G   GVY+G EYG+ERIRG RDWKNAM+ GA TGA++SA  
Sbjct: 61  STLEHALKKLC--KEGVYWGAAGGVYIGTEYGIERIRGSRDWKNAMLAGAATGAVLSAVG 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFIN 146
              +D IVIDAI GGA+ATA++F+N
Sbjct: 121 KKGKDTIVIDAILGGALATASQFVN 143

[Close]

BLAST of Carg20659-RA vs. ExPASy Swiss-Prot
Match: Q0WMZ5 (Outer envelope pore protein 16-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP162 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 6.3e-12
Identity = 46/155 (29.68%), Postives = 79/155 (50.97%), Query Frame = 0


Query: 18  VIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGT---------------ISSHN 77
           + D+G+P LN   D F+K   V A +A + +AY  V  G                   H 
Sbjct: 21  LFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGAGFDSNNVGPPSEITGNKKHR 80

Query: 78  F-----ENTVSVSCFV----SQLVESGTVAGVYVGMEYGVERIR-GRRDWKNAMIGGALT 137
           F     E++ S+   V     + ++ G  AG+Y G+ YG+  +R G  DW+N+ + GALT
Sbjct: 81  FPNLRGESSKSLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRGGAHDWRNSAVAGALT 140

Query: 138 GA--MVSAASNSNRDKIVIDAITGGAVATAAEFIN 146
           GA   ++ +  ++ +++V  A+TG A++TAA  ++
Sbjct: 141 GAAMAMTTSERTSHEQVVQSALTGAAISTAANLLS 175

[Close]

BLAST of Carg20659-RA vs. ExPASy Swiss-Prot
Match: Q9LZH8 (Outer envelope pore protein 16-4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP164 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 5.7e-05
Identity = 36/113 (31.86%), Postives = 57/113 (50.44%), Query Frame = 0


Query: 24  PFLNLTVDGFLKI---GSVAATRAAAEDAYHVVRKGTISSHNFENTVSVSCFVSQLV--- 83
           P  +LTV+  L++   G +    A   DA  +   G          VS + FV++ +   
Sbjct: 10  PCSSLTVESVLRVATAGGLYGLCAGPRDARKIGLSG----------VSQASFVAKSIGRF 69

Query: 84  --ESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAASNSNRDKIV 129
             + G V+GV+     G++R RG+ DW NA++GGA+ GA V A S  N  ++V
Sbjct: 70  GFQCGLVSGVFTMTHCGLQRYRGKNDWVNALVGGAVAGAAV-AISTRNWTQVV 111

[Close]

BLAST of Carg20659-RA vs. ExPASy TrEMBL
Match: A0A6J1HZI5 (outer envelope pore protein 16, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111469516 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.6e-66
Identity = 136/148 (91.89%), Postives = 138/148 (93.24%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS
Sbjct: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           HNFENT+   C   +    GTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS
Sbjct: 61  HNFENTLKKMC--KEGAYWGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           NSNRDKIVIDAITGGAVATAAEFINYLT
Sbjct: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 146

[Close]

BLAST of Carg20659-RA vs. ExPASy TrEMBL
Match: A0A6J1HJY9 (outer envelope pore protein 16, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464797 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.6e-66
Identity = 136/148 (91.89%), Postives = 138/148 (93.24%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS
Sbjct: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           HNFENT+   C   +    GTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS
Sbjct: 61  HNFENTLKKMC--KEGAYWGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           NSNRDKIVIDAITGGAVATAAEFINYLT
Sbjct: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 146

[Close]

BLAST of Carg20659-RA vs. ExPASy TrEMBL
Match: A0A5A7VKK1 (Outer envelope pore protein 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold352G00490 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.3e-65
Identity = 133/148 (89.86%), Postives = 140/148 (94.59%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIS+
Sbjct: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIST 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           HNFENTVS+SCF  +L  +  VAG YVGMEYGVERIRG RDWKNAMIGGALTGA+VSAAS
Sbjct: 61  HNFENTVSISCFRLKLWRA--VAGAYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           N+NRDK+VIDAITGGAVATAAEFINYLT
Sbjct: 121 NNNRDKVVIDAITGGAVATAAEFINYLT 146

[Close]

BLAST of Carg20659-RA vs. ExPASy TrEMBL
Match: A0A5D3BDN0 (Outer envelope pore protein 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002350 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.7e-63
Identity = 128/148 (86.49%), Postives = 136/148 (91.89%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIS+
Sbjct: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIST 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           HNFENT+   C  ++    G VAG YVGMEYGVER+RG RDWKNAMIGGALTGA+VSAAS
Sbjct: 61  HNFENTLKKMC--TEGAYWGAVAGAYVGMEYGVERVRGTRDWKNAMIGGALTGALVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           N+NRDK+VIDAITGGAVATAAEFINYLT
Sbjct: 121 NNNRDKVVIDAITGGAVATAAEFINYLT 146

[Close]

BLAST of Carg20659-RA vs. ExPASy TrEMBL
Match: A0A1S3C572 (outer envelope pore protein 16, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103497163 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.7e-63
Identity = 128/148 (86.49%), Postives = 136/148 (91.89%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIS+
Sbjct: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTIST 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
           HNFENT+   C  ++    G VAG YVGMEYGVER+RG RDWKNAMIGGALTGA+VSAAS
Sbjct: 61  HNFENTLKKMC--TEGAYWGAVAGAYVGMEYGVERVRGTRDWKNAMIGGALTGALVSAAS 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFINYLT 149
           N+NRDK+VIDAITGGAVATAAEFINYLT
Sbjct: 121 NNNRDKVVIDAITGGAVATAAEFINYLT 146

[Close]

BLAST of Carg20659-RA vs. TAIR 10
Match: AT2G28900.1 (outer plastid envelope protein 16-1 )

HSP 1 Score: 165.2 bits (417), Expect = 3.9e-41
Identity = 80/145 (55.17%), Postives = 104/145 (71.72%), Query Frame = 0


Query: 1   MPRTGFVGSLSTPKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS 60
           MP + F G++STPK+ V +DMGNPFLNLTVD FLKIG+V  T++ AED Y  + KG++S 
Sbjct: 1   MPSSTFSGTVSTPKLSVAVDMGNPFLNLTVDAFLKIGAVGVTKSLAEDTYKAIDKGSLSK 60

Query: 61  HNFENTVSVSCFVSQLVESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAAS 120
              E+ +   C   + V  G   GVY+G EYG+ERIRG RDWKNAM+ GA TGA++SA  
Sbjct: 61  STLEHALKKLC--KEGVYWGAAGGVYIGTEYGIERIRGSRDWKNAMLAGAATGAVLSAVG 120

Query: 121 NSNRDKIVIDAITGGAVATAAEFIN 146
              +D IVIDAI GGA+ATA++F+N
Sbjct: 121 KKGKDTIVIDAILGGALATASQFVN 143

[Close]

BLAST of Carg20659-RA vs. TAIR 10
Match: AT4G16160.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.2e-13
Identity = 47/153 (30.72%), Postives = 81/153 (52.94%), Query Frame = 0


Query: 18  VIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISS-------------HNF- 77
           + D+G+P LN   D F+K   V A +A + +AY  V  G  S+             H F 
Sbjct: 21  LFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGFDSNNVGPPSEITGNKKHRFP 80

Query: 78  ----ENTVSVSCFV----SQLVESGTVAGVYVGMEYGVERIR-GRRDWKNAMIGGALTGA 137
               E++ S+   V     + ++ G  AG+Y G+ YG+  +R G  DW+N+ + GALTGA
Sbjct: 81  NLRGESSKSLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRGGAHDWRNSAVAGALTGA 140

Query: 138 --MVSAASNSNRDKIVIDAITGGAVATAAEFIN 146
              ++ +  ++ +++V  A+TG A++TAA  ++
Sbjct: 141 AMAMTTSERTSHEQVVQSALTGAAISTAANLLS 173

[Close]

BLAST of Carg20659-RA vs. TAIR 10
Match: AT4G16160.2 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 72.0 bits (175), Expect = 4.5e-13
Identity = 46/155 (29.68%), Postives = 79/155 (50.97%), Query Frame = 0


Query: 18  VIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGT---------------ISSHN 77
           + D+G+P LN   D F+K   V A +A + +AY  V  G                   H 
Sbjct: 21  LFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGAGFDSNNVGPPSEITGNKKHR 80

Query: 78  F-----ENTVSVSCFV----SQLVESGTVAGVYVGMEYGVERIR-GRRDWKNAMIGGALT 137
           F     E++ S+   V     + ++ G  AG+Y G+ YG+  +R G  DW+N+ + GALT
Sbjct: 81  FPNLRGESSKSLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRGGAHDWRNSAVAGALT 140

Query: 138 GA--MVSAASNSNRDKIVIDAITGGAVATAAEFIN 146
           GA   ++ +  ++ +++V  A+TG A++TAA  ++
Sbjct: 141 GAAMAMTTSERTSHEQVVQSALTGAAISTAANLLS 175

[Close]

BLAST of Carg20659-RA vs. TAIR 10
Match: AT3G62880.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 48.9 bits (115), Expect = 4.1e-06
Identity = 36/113 (31.86%), Postives = 57/113 (50.44%), Query Frame = 0


Query: 24  PFLNLTVDGFLKI---GSVAATRAAAEDAYHVVRKGTISSHNFENTVSVSCFVSQLV--- 83
           P  +LTV+  L++   G +    A   DA  +   G          VS + FV++ +   
Sbjct: 10  PCSSLTVESVLRVATAGGLYGLCAGPRDARKIGLSG----------VSQASFVAKSIGRF 69

Query: 84  --ESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAASNSNRDKIV 129
             + G V+GV+     G++R RG+ DW NA++GGA+ GA V A S  N  ++V
Sbjct: 70  GFQCGLVSGVFTMTHCGLQRYRGKNDWVNALVGGAVAGAAV-AISTRNWTQVV 111

[Close]

BLAST of Carg20659-RA vs. TAIR 10
Match: AT3G62880.2 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 48.9 bits (115), Expect = 4.1e-06
Identity = 36/113 (31.86%), Postives = 57/113 (50.44%), Query Frame = 0


Query: 24  PFLNLTVDGFLKI---GSVAATRAAAEDAYHVVRKGTISSHNFENTVSVSCFVSQLV--- 83
           P  +LTV+  L++   G +    A   DA  +   G          VS + FV++ +   
Sbjct: 10  PCSSLTVESVLRVATAGGLYGLCAGPRDARKIGLSG----------VSQASFVAKSIGRF 69

Query: 84  --ESGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAASNSNRDKIV 129
             + G V+GV+     G++R RG+ DW NA++GGA+ GA V A S  N  ++V
Sbjct: 70  GFQCGLVSGVFTMTHCGLQRYRGKNDWVNALVGGAVAGAAV-AISTRNWTQVV 111

The following BLAST results are available for this feature:

BLAST of Carg20659-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019193.1	3.3e-74	100.00	Outer envelope pore protein 16, chloroplastic [Cucurbita argyrosperma subsp. arg...	[more]
XP_022964801.1	9.5e-66	91.89	outer envelope pore protein 16, chloroplastic [Cucurbita moschata] >XP_022970582...	[more]
KAA0067730.1	2.8e-65	89.86	outer envelope pore protein 16 [Cucumis melo var. makuwa]	[more]
XP_038893818.1	8.9e-64	87.84	outer envelope pore protein 16, chloroplastic [Benincasa hispida]	[more]
XP_008457483.1	7.6e-63	86.49	PREDICTED: outer envelope pore protein 16, chloroplastic [Cucumis melo] >TYJ9741...	[more]

BLAST of Carg20659-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41050	2.7e-55	71.62	Outer envelope pore protein 16, chloroplastic OS=Pisum sativum OX=3888 GN=OEP16 ...	[more]
Q9ZV24	5.5e-40	55.17	Outer envelope pore protein 16-1, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q0WMZ5	6.3e-12	29.68	Outer envelope pore protein 16-2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9LZH8	5.7e-05	31.86	Outer envelope pore protein 16-4, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Carg20659-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HZI5	4.6e-66	91.89	outer envelope pore protein 16, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1HJY9	4.6e-66	91.89	outer envelope pore protein 16, chloroplastic OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A5A7VKK1	1.3e-65	89.86	Outer envelope pore protein 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A5D3BDN0	3.7e-63	86.49	Outer envelope pore protein 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3C572	3.7e-63	86.49	outer envelope pore protein 16, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034...	[more]

BLAST of Carg20659-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28900.1	3.9e-41	55.17	outer plastid envelope protein 16-1	[more]
AT4G16160.1	1.2e-13	30.72	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT4G16160.2	4.5e-13	29.68	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT3G62880.1	4.1e-06	31.86	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT3G62880.2	4.1e-06	31.86	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF02466	Tim17	coord: 78..137 e-value: 1.9E-9 score: 37.9
None	No IPR available	PANTHER	PTHR15371	TIM23	coord: 1..146
None	No IPR available	PANTHER	PTHR15371:SF2	OUTER ENVELOPE PORE PROTEIN 16-1, CHLOROPLASTIC	coord: 1..146

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg20659	Carg20659	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20659-RA:1	Carg20659-RA:1	exon
Carg20659-RA:2	Carg20659-RA:2	exon
Carg20659-RA:3	Carg20659-RA:3	exon
Carg20659-RA:4	Carg20659-RA:4	exon
Carg20659-RA:5	Carg20659-RA:5	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20659-RA:five_prime_utr	Carg20659-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20659-RA:cds	Carg20659-RA:cds	CDS
Carg20659-RA:cds	Carg20659-RA:cds_2	CDS
Carg20659-RA:cds	Carg20659-RA:cds_3	CDS
Carg20659-RA:cds	Carg20659-RA:cds_4	CDS
Carg20659-RA:cds	Carg20659-RA:cds_5	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20659-RA:three_prime_utr	Carg20659-RA:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg20659-RA	Carg20659-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0003333	amino acid transmembrane transport
biological_process	GO:0045037	protein import into chloroplast stroma
cellular_component	GO:0009707	chloroplast outer membrane
molecular_function	GO:0015171	amino acid transmembrane transporter activity