Carg20366-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20366-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Reticulon-like protein
Location	Carg_Chr18: 11630579 .. 11632599 (+)
Sequence length	769
RNA-Seq Expression	Carg20366-RA
Synteny	Carg20366-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCTCTGCTTCCATTTTCTCTTGTTCAATTTTGCTGCAAAGATCCATGGCTGACCAACCACGCGAGCGGGATTCGGTTATCGACAAGATTTCCGATAAGATCGGCGACTGCATTTCATCGTCTTCATCCGACTCTGATGATGGCCTGCATTCCAAAATCGATGCTGTAAAATCCCGTGTTTTTAGGCTCTTCGGTAGAGATAAGCCCGTCCACACCGTTCTGGGCGGTGGAAAGCGTACTCTCTGATCTCTTTCTCTCGTGGTTGATCGGCTAATGTAGAAAAAATTAGAAGTTTTGATGAATGTATACGTTTTGGATCATGCTCTGTTACTTTTATTTATCCAATGCCCTTCTATATTTAATTGTTATTATCGATATGTGTTTGCGTGAGTTTGTGCTAATTTTCGTTCGATTGGATGACCGTCCAATGTTGCAACAGAGAGTTCGTTATCTCTATCTTTCAATCGAGTTATGCATGAAAGCCTTACGTTATTAGCGTTATGGCTTCACAGTTTGTGGTTTCAGGTAGATCTCTCATCAGGAAATAGCTCAGTTACTGGTTAGATTTATATTTTAGTTCTTAAGAATATTGTAGTTGACGGGACAAACGGCTGAAGGAGAAAAGTCTAGTTCTTGTTTGGTCTGTCTACTAAACGACTGCAAAAACATTGCCTACCCATTATTTGTAGTCTATTAGTGCAATTGTTTGAAGAATTTTCTGAAAGCTTTCGCGGAAATGTATCCCAAAGTAGTGACAATTAGATGCATGCACCCTTCCTCTGTATTGAAAATTGTGTCATAGGCTCAAATGTTTGCATGATTAGCTTTGTTTATGAATTACGATCTTTTATTGGTGTTCTTTGAATTGTTTTAGTATGAGATCGTACGACATTTTCTTTTTCTTTTTATTTTATTATTTTATTATTATTTGGTATAACAACTGCATTAAGATAGATTGAGAACTGATTCGACATATAATTTTTTTCTCCCAAACATTTCTATAGAACATGACAATTGCTGAAGTATGACCTTCTACAGTTCTGTTCCTCACCCCTTTTCCTGTCTTTATTCGTGAGGGAACTTTTCTTGTAAGTTATGATTGCCTCCTGTTGCAGCTGCTGATGTTTTTCTTTGGAGGAACAAGAAGAACTCTGCAGGCATCCTTGGGGTTGCCACCGCCTTGTGGATTGTTTTTGAGCTGATTGAGTACCAATTAATCACCCTCGTGTGCCATTTATTAATTCTTTTACTTGCTATAATGTTCTTGTGGTCTTATGCCAATACGTTTATCAACAAGTAAGGACTTCGGCTTACAATATCTAATGCAATATATAGTCTGTTGGTTTTGTTTTTCCCTTTTATGTATATATTTTTGGTGTTGTTTGTATAGGACCCCACCTCAAATCCCAGATATGAAACTCCCTGAAGTTTGCTTTCTTCAATTTGTCTCTGCACTGAGAATCGAAATCAACCGTGCAATCTCCATTTTACGTGGTATTGCTTGTGGGAGAGATTTGAAGAAGTTTCTCAGTGTACGTTCACTGCTACTATCACGAGTAAATGTGTTTTAGATTTGAAGAAACAAAATGAATGTTTCTAGGCTCTTTTTTTTTTTTTTTTGAAATTGCTTTTGATAGTTTGATGCAATTAACAGGTTTTTCTTGGTTTATGGGTTCTATCAGTAGTTGGCAGTTGGTGCAATTTTCTGACCTTGTTATACATATGTAAGGAATTCTACACCGTCAGTTATTTGGATGAGAAATCTCCAAAAATTTTGCATTTATAACTGAAATTATATGTGTTATACCTATTGATATTCTGGTGTGCTATTGATTCTTGAAAATCAGCCATTGTATTGCTACACACGGTGCCAGTGTTTTATGAGAAGTATGAAGACCAGGTTGACCCATTGGCTGAGAAAGCTTGGATAGAGTTCAAGAAGCAGTATGCTGTATTCGATGCCAAGGTTCTAAGTAAAATCCCCATTGGGGCACTTCAAGAGAAGAAGAAGAAGAATTAG

mRNA sequence

TTTCTCTGCTTCCATTTTCTCTTGTTCAATTTTGCTGCAAAGATCCATGGCTGACCAACCACGCGAGCGGGATTCGGTTATCGACAAGATTTCCGATAAGATCGGCGACTGCATTTCATCGTCTTCATCCGACTCTGATGATGGCCTGCATTCCAAAATCGATGCTGTAAAATCCCGTGTTTTTAGGCTCTTCGGTAGAGATAAGCCCGTCCACACCGTTCTGGGCGGTGGAAAGCCTGCTGATGTTTTTCTTTGGAGGAACAAGAAGAACTCTGCAGGCATCCTTGGGGTTGCCACCGCCTTGTGGATTGTTTTTGAGCTGATTGAGTACCAATTAATCACCCTCGTGTGCCATTTATTAATTCTTTTACTTGCTATAATGTTCTTGTGGTCTTATGCCAATACGTTTATCAACAAGACCCCACCTCAAATCCCAGATATGAAACTCCCTGAAGTTTGCTTTCTTCAATTTGTCTCTGCACTGAGAATCGAAATCAACCGTGCAATCTCCATTTTACGTGGTATTGCTTGTGGGAGAGATTTGAAGAAGTTTCTCAGTGTTTTTCTTGGTTTATGGGTTCTATCAGTAGTTGGCACCATTGTATTGCTACACACGGTGCCAGTGTTTTATGAGAAGTATGAAGACCAGGTTGACCCATTGGCTGAGAAAGCTTGGATAGAGTTCAAGAAGCAGTATGCTGTATTCGATGCCAAGGTTCTAAGTAAAATCCCCATTGGGGCACTTCAAGAGAAGAAGAAGAAGAATTAG

Coding sequence (CDS)

ATGGCTGACCAACCACGCGAGCGGGATTCGGTTATCGACAAGATTTCCGATAAGATCGGCGACTGCATTTCATCGTCTTCATCCGACTCTGATGATGGCCTGCATTCCAAAATCGATGCTGTAAAATCCCGTGTTTTTAGGCTCTTCGGTAGAGATAAGCCCGTCCACACCGTTCTGGGCGGTGGAAAGCCTGCTGATGTTTTTCTTTGGAGGAACAAGAAGAACTCTGCAGGCATCCTTGGGGTTGCCACCGCCTTGTGGATTGTTTTTGAGCTGATTGAGTACCAATTAATCACCCTCGTGTGCCATTTATTAATTCTTTTACTTGCTATAATGTTCTTGTGGTCTTATGCCAATACGTTTATCAACAAGACCCCACCTCAAATCCCAGATATGAAACTCCCTGAAGTTTGCTTTCTTCAATTTGTCTCTGCACTGAGAATCGAAATCAACCGTGCAATCTCCATTTTACGTGGTATTGCTTGTGGGAGAGATTTGAAGAAGTTTCTCAGTGTTTTTCTTGGTTTATGGGTTCTATCAGTAGTTGGCACCATTGTATTGCTACACACGGTGCCAGTGTTTTATGAGAAGTATGAAGACCAGGTTGACCCATTGGCTGAGAAAGCTTGGATAGAGTTCAAGAAGCAGTATGCTGTATTCGATGCCAAGGTTCTAAGTAAAATCCCCATTGGGGCACTTCAAGAGAAGAAGAAGAAGAATTAG

Protein sequence

MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLGGGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANTFINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLSVVGTIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIPIGALQEKKKKN

Homology

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BLAST of Carg20366-RA vs. NCBI nr
Match: KAG7013283.1 (Reticulon-like protein B5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 468.8 bits (1205), Expect = 2.9e-128
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60
           MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG
Sbjct: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60

Query: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120
           GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT
Sbjct: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120

Query: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180
           FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS
Sbjct: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180

Query: 181 VVGTIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIPIGALQEKKKKN 240
           VVGTIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIPIGALQEKKKKN
Sbjct: 181 VVGTIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIPIGALQEKKKKN 240

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BLAST of Carg20366-RA vs. NCBI nr
Match: XP_022945796.1 (reticulon-like protein B1 [Cucurbita moschata])

HSP 1 Score: 458.8 bits (1179), Expect = 3.0e-125
Identity = 239/251 (95.22%), Postives = 240/251 (95.62%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60
           MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG
Sbjct: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60

Query: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120
           GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT
Sbjct: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120

Query: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180
           FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS
Sbjct: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180

Query: 181 VVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 240
           VVG           +IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP
Sbjct: 181 VVGSWCNFLTLLYISIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 240

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BLAST of Carg20366-RA vs. NCBI nr
Match: XP_022968328.1 (reticulon-like protein B1 [Cucurbita maxima])

HSP 1 Score: 457.2 bits (1175), Expect = 8.6e-125
Identity = 238/251 (94.82%), Postives = 240/251 (95.62%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60
           MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG
Sbjct: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60

Query: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120
           GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT
Sbjct: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120

Query: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180
           FINKTPPQIPD+KLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS
Sbjct: 121 FINKTPPQIPDVKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180

Query: 181 VVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 240
           VVG           +IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP
Sbjct: 181 VVGSWCNFLTLLYISIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 240

[Close]

BLAST of Carg20366-RA vs. NCBI nr
Match: XP_023541342.1 (reticulon-like protein B1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 456.4 bits (1173), Expect = 1.5e-124
Identity = 238/251 (94.82%), Postives = 239/251 (95.22%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60
           MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG
Sbjct: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60

Query: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120
            GKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT
Sbjct: 61  AGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120

Query: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180
           FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS
Sbjct: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180

Query: 181 VVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 240
           VVG           +IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP
Sbjct: 181 VVGSWCNFLTLLYISIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 240

[Close]

BLAST of Carg20366-RA vs. NCBI nr
Match: KAG6574214.1 (Reticulon-like protein B5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 427.9 bits (1099), Expect = 5.6e-116
Identity = 222/234 (94.87%), Postives = 223/234 (95.30%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60
           MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG
Sbjct: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60

Query: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120
           GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT
Sbjct: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120

Query: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180
           FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS
Sbjct: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180

Query: 181 VVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAK 224
           VVG           +IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAK
Sbjct: 181 VVGSWCNFLTLLYISIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAK 234

[Close]

BLAST of Carg20366-RA vs. ExPASy Swiss-Prot
Match: Q9SUR3 (Reticulon-like protein B1 OS=Arabidopsis thaliana OX=3702 GN=RTNLB1 PE=1 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-74
Identity = 149/253 (58.89%), Postives = 187/253 (73.91%), Query Frame = 0


Query: 7   ERDSVIDKISDKI---GDCISSSSSDSDDGLHSKI-------DAVKSRVFRLFGRDKPVH 66
           ER+S++DKIS+KI   GD  SSSSS  D+    K         ++KS+V+RLFGR++PVH
Sbjct: 22  ERESLMDKISEKIHHGGDSSSSSSSSDDEDEKKKTKKPSSPSSSMKSKVYRLFGREQPVH 81

Query: 67  TVLGGGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWS 126
            VLGGGKPAD+F+W+NKK S G+LG ATA W+VFEL+EY L+TL+CH++I++LA++FLWS
Sbjct: 82  KVLGGGKPADIFMWKNKKMSGGVLGGATAAWVVFELMEYHLLTLLCHVMIVVLAVLFLWS 141

Query: 127 YANTFINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGL 186
            A  FINK+PP+IP++ +PE   LQ  S LRIEINR  S LR IA GRDLKKFL    GL
Sbjct: 142 NATMFINKSPPKIPEVHIPEEPILQLASGLRIEINRGFSSLREIASGRDLKKFLIAIAGL 201

Query: 187 WVLSVVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVL 239
           WVLS++G            +VLL TVP+ Y+KYED+VDPL EKA IE KKQYAV D KVL
Sbjct: 202 WVLSILGGCFNFLTLAYIALVLLFTVPLAYDKYEDKVDPLGEKAMIELKKQYAVLDEKVL 261

[Close]

BLAST of Carg20366-RA vs. ExPASy Swiss-Prot
Match: O82352 (Reticulon-like protein B5 OS=Arabidopsis thaliana OX=3702 GN=RTNLB5 PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 2.6e-71
Identity = 141/241 (58.51%), Postives = 182/241 (75.52%), Query Frame = 0


Query: 9   DSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLGGGKPADVF 68
           +S+++KIS+KI    SSSSS+S+        AVK++++R+FGR+KPVH VLGGGKPADVF
Sbjct: 13  ESLMEKISEKIHHHDSSSSSESEYEKPDSPSAVKAKIYRMFGREKPVHKVLGGGKPADVF 72

Query: 69  LWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANTFINKTPPQ 128
           LWR+KK S  +LGVATA+W++FEL+EY L++L+CH+ IL L  +FLWS A+T INKT PQ
Sbjct: 73  LWRDKKLSGAVLGVATAIWVLFELVEYHLLSLLCHISILALGGLFLWSNAHTLINKTSPQ 132

Query: 129 IPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLSVVGT---- 188
           IP++ +PE  FL   S+LR E+N+A  ILR IA GRDLKKFL V +GLW++SVVG     
Sbjct: 133 IPEIHVPEEAFLVVASSLRNELNQAFVILRSIALGRDLKKFLMVVVGLWIISVVGNWFNF 192

Query: 189 -------IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIPIGALQEKK 239
                   V+LHTVP+ YEK+ED+VDPLAEKA  E +KQY VFD KVLSKIPI +L+ K 
Sbjct: 193 LTLVYICFVILHTVPMLYEKHEDKVDPLAEKAMKELQKQYVVFDEKVLSKIPIASLKAKA 252

[Close]

BLAST of Carg20366-RA vs. ExPASy Swiss-Prot
Match: Q9SUT9 (Reticulon-like protein B2 OS=Arabidopsis thaliana OX=3702 GN=RTNLB2 PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 3.3e-71
Identity = 141/249 (56.63%), Postives = 185/249 (74.30%), Query Frame = 0


Query: 7   ERDSVIDKISDKI---GDCISSSSSDSDD---GLHSKIDAVKSRVFRLFGRDKPVHTVLG 66
           ER+S+++K+S+KI   GD  SSSSSD ++      S   ++KS+V+RLFGR++PVH VLG
Sbjct: 22  ERESLMEKLSEKIHHKGDSSSSSSSDDENEKKSSSSSPKSLKSKVYRLFGRERPVHKVLG 81

Query: 67  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 126
           GGKPAD+F+W++KK S G+ G AT  W++FEL+EY L+TL+CH++I+ LA++FLWS A  
Sbjct: 82  GGKPADIFMWKDKKMSGGVFGGATVAWVLFELMEYHLLTLLCHVMIVALAVLFLWSNATM 141

Query: 127 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 186
           FI+K+PP+IP++ +PE   LQ  S LRIEINR IS LR IA GRD+KKFLS   GLWVLS
Sbjct: 142 FIHKSPPKIPEVHIPEEPLLQLASGLRIEINRGISSLREIASGRDIKKFLSAIAGLWVLS 201

Query: 187 VVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 239
           ++G            +VLL TVP+FY+KYED+VD   EKA  E KKQYAV DAKV SKIP
Sbjct: 202 ILGGCYSFLTLAYIALVLLFTVPLFYDKYEDKVDSYGEKAMAELKKQYAVLDAKVFSKIP 261

[Close]

BLAST of Carg20366-RA vs. ExPASy Swiss-Prot
Match: Q6DBN4 (Reticulon-like protein B6 OS=Arabidopsis thaliana OX=3702 GN=RTNLB6 PE=1 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 3.7e-70
Identity = 138/241 (57.26%), Postives = 180/241 (74.69%), Query Frame = 0


Query: 7   ERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLGGGKPAD 66
           + +S+++KI+DKI D  SSSSSDS+        A+K++++RLFGR+KPVH VLGGG PAD
Sbjct: 11  KEESLMEKIADKIHDHDSSSSSDSEHEKPESPSALKAKIYRLFGREKPVHKVLGGGLPAD 70

Query: 67  VFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANTFINKTP 126
           VFLWR+KK SA +LGVATA+W++FEL+EY  ++LVCH+LI  LA +FL S A+ F+NKTP
Sbjct: 71  VFLWRDKKLSASVLGVATAIWVLFELVEYHFLSLVCHILIFALAALFLLSNAHAFMNKTP 130

Query: 127 PQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLSVVGT-- 186
           P+IP++ + E  FL  VSALR E+N+A  ILR IA GRDLKKFL V  GLW++SVVG   
Sbjct: 131 PKIPEIHIKEEHFLMIVSALRNELNQAFVILRSIALGRDLKKFLMVVFGLWIISVVGNWF 190

Query: 187 ---------IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIPIGALQE 237
                     V+LHTVP+ YEK+ED+VDP+AEK   E KK Y VFD KVLSK+P+ +L+ 
Sbjct: 191 NFLTLVYICFVVLHTVPMLYEKHEDKVDPVAEKTLKELKKHYMVFDEKVLSKLPVASLKA 250

[Close]

BLAST of Carg20366-RA vs. ExPASy Swiss-Prot
Match: Q9SH59 (Reticulon-like protein B3 OS=Arabidopsis thaliana OX=3702 GN=RTNLB3 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 2.9e-67
Identity = 143/257 (55.64%), Postives = 182/257 (70.82%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKI--GDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTV 60
           MA++ +  +S+++KIS+KI   D  SSSSSDSDD  +S   ++K++++RLFGR++P+H +
Sbjct: 1   MAEEHKHEESIMEKISEKIHGHDDSSSSSSDSDDDKNSA--SLKTKIYRLFGREQPLHKL 60

Query: 61  LGGGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYA 120
            GGGKPAD+FLWRNKK S G+LG AT  WI+FEL+EY L+TL  H+ IL LA++FLWS A
Sbjct: 61  FGGGKPADIFLWRNKKVSGGVLGAATVSWILFELLEYNLLTLFGHISILALAVLFLWSSA 120

Query: 121 NTFINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWV 180
           +TFI+K+P  IP++ +PE   LQ  S LRIEINR  ++LR IA GRDLKKFL V  GLWV
Sbjct: 121 STFIHKSPLHIPEVHIPEDVVLQLASGLRIEINRGFTVLRDIASGRDLKKFLLVIAGLWV 180

Query: 181 LSVVGT-----------IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVL-- 240
           LS VG+            VLL T+PV YEKYED+VD   EKA  E KKQY  FD KVL  
Sbjct: 181 LSKVGSSCNFLTLIYIATVLLFTIPVLYEKYEDKVDDFGEKAMREIKKQYVEFDVKVLSK 240

[Close]

BLAST of Carg20366-RA vs. ExPASy TrEMBL
Match: A0A6J1G1W3 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111449937 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.4e-125
Identity = 239/251 (95.22%), Postives = 240/251 (95.62%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60
           MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG
Sbjct: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60

Query: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120
           GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT
Sbjct: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120

Query: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180
           FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS
Sbjct: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180

Query: 181 VVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 240
           VVG           +IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP
Sbjct: 181 VVGSWCNFLTLLYISIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 240

[Close]

BLAST of Carg20366-RA vs. ExPASy TrEMBL
Match: A0A6J1HXQ1 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111467592 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 4.2e-125
Identity = 238/251 (94.82%), Postives = 240/251 (95.62%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60
           MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG
Sbjct: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60

Query: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120
           GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT
Sbjct: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120

Query: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180
           FINKTPPQIPD+KLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS
Sbjct: 121 FINKTPPQIPDVKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180

Query: 181 VVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 240
           VVG           +IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP
Sbjct: 181 VVGSWCNFLTLLYISIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 240

[Close]

BLAST of Carg20366-RA vs. ExPASy TrEMBL
Match: A0A0A0L7V4 (Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G363160 PE=4 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.4e-96
Identity = 189/249 (75.90%), Postives = 207/249 (83.13%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60
           MADQP E DSVID  S KI D ISSSSSDSDD   SK DAVK  V+RLFGRDKPVHTVLG
Sbjct: 1   MADQPAEPDSVIDNFSAKIDDHISSSSSDSDDDHPSKPDAVKCHVYRLFGRDKPVHTVLG 60

Query: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120
           GGKPADVFLW+NKKNSAG+LG ATALWI+FEL+EYQLITLVCH+LI+LLAI FLWSYANT
Sbjct: 61  GGKPADVFLWKNKKNSAGVLGGATALWILFELLEYQLITLVCHILIILLAIPFLWSYANT 120

Query: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180
           FINKTPPQIPD++LPE C LQ V++LRIEINR IS LR +A GRDL+KFLS FLGLW+LS
Sbjct: 121 FINKTPPQIPDVRLPEDCLLQVVTSLRIEINRIISTLRDVASGRDLRKFLSAFLGLWILS 180

Query: 181 VVGT-----------IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 239
           + G+            +LLHTVPV YEKYEDQVDP AEKA IE KKQYA FDAKVLSKIP
Sbjct: 181 IAGSWCNFLTLLYMCFILLHTVPVLYEKYEDQVDPFAEKALIELKKQYAEFDAKVLSKIP 240

[Close]

BLAST of Carg20366-RA vs. ExPASy TrEMBL
Match: A0A6J1EA75 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430763 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.4e-88
Identity = 183/249 (73.49%), Postives = 204/249 (81.93%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60
           MADQP   DSVI+KIS KI    SSSSS SDDG  SKI+AVKS VFRLFGRDKPVHTVLG
Sbjct: 1   MADQPGGLDSVINKISGKIDGYNSSSSSGSDDGHPSKINAVKSHVFRLFGRDKPVHTVLG 60

Query: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120
           GGKPADV LWRNKKNSAGILG ATALWI+FEL+EYQLITLVCH+LIL+LA++F  SYA++
Sbjct: 61  GGKPADVLLWRNKKNSAGILGGATALWILFELLEYQLITLVCHILILVLALVFTRSYASS 120

Query: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180
           FINK+PPQIPD +LPE   L+ VSALRIEIN  IS LR IA GRDLKKFL  F+GLWVLS
Sbjct: 121 FINKSPPQIPDFRLPEDRLLEVVSALRIEINHVISNLRNIASGRDLKKFLRAFIGLWVLS 180

Query: 181 VVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 239
           +VG           ++VLLHTVPV YEKY+DQVD  AEKA IE KKQYAVF A+VLSKIP
Sbjct: 181 MVGSWCNFLTLLYTSVVLLHTVPVLYEKYDDQVDAFAEKALIEVKKQYAVFSAEVLSKIP 240

[Close]

BLAST of Carg20366-RA vs. ExPASy TrEMBL
Match: A0A6J1JBF9 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482938 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 4.1e-88
Identity = 182/249 (73.09%), Postives = 203/249 (81.53%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLG 60
           MADQP   DSV DKIS KI    SSSSS SDD   SKI+AVKS VFRLFGRDKPVHTVLG
Sbjct: 1   MADQPGGLDSVTDKISGKIDGYNSSSSSGSDDDQPSKINAVKSHVFRLFGRDKPVHTVLG 60

Query: 61  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 120
           GGKPADV LWRNKKNSAGILG ATALWI+FEL+EYQLITL+CH+LIL+LA++F  SYA++
Sbjct: 61  GGKPADVLLWRNKKNSAGILGGATALWILFELLEYQLITLLCHILILVLALVFTRSYASS 120

Query: 121 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 180
           FINK+PPQIPD +LPE   L+ VSALRIEIN  IS LR IA GRDLKKFL  F+GLWVLS
Sbjct: 121 FINKSPPQIPDFRLPEDRLLEVVSALRIEINHVISNLRNIASGRDLKKFLRAFIGLWVLS 180

Query: 181 VVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 239
           +VG           ++VLLHTVPV YEKY+DQVD  AEKA IE KKQYAVF+A+VLSKIP
Sbjct: 181 MVGSWCNFLTLLYTSVVLLHTVPVLYEKYDDQVDAFAEKALIEVKKQYAVFNAEVLSKIP 240

[Close]

BLAST of Carg20366-RA vs. TAIR 10
Match: AT4G23630.1 (VIRB2-interacting protein 1 )

HSP 1 Score: 280.8 bits (717), Expect = 1.0e-75
Identity = 149/253 (58.89%), Postives = 187/253 (73.91%), Query Frame = 0


Query: 7   ERDSVIDKISDKI---GDCISSSSSDSDDGLHSKI-------DAVKSRVFRLFGRDKPVH 66
           ER+S++DKIS+KI   GD  SSSSS  D+    K         ++KS+V+RLFGR++PVH
Sbjct: 22  ERESLMDKISEKIHHGGDSSSSSSSSDDEDEKKKTKKPSSPSSSMKSKVYRLFGREQPVH 81

Query: 67  TVLGGGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWS 126
            VLGGGKPAD+F+W+NKK S G+LG ATA W+VFEL+EY L+TL+CH++I++LA++FLWS
Sbjct: 82  KVLGGGKPADIFMWKNKKMSGGVLGGATAAWVVFELMEYHLLTLLCHVMIVVLAVLFLWS 141

Query: 127 YANTFINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGL 186
            A  FINK+PP+IP++ +PE   LQ  S LRIEINR  S LR IA GRDLKKFL    GL
Sbjct: 142 NATMFINKSPPKIPEVHIPEEPILQLASGLRIEINRGFSSLREIASGRDLKKFLIAIAGL 201

Query: 187 WVLSVVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVL 239
           WVLS++G            +VLL TVP+ Y+KYED+VDPL EKA IE KKQYAV D KVL
Sbjct: 202 WVLSILGGCFNFLTLAYIALVLLFTVPLAYDKYEDKVDPLGEKAMIELKKQYAVLDEKVL 261

[Close]

BLAST of Carg20366-RA vs. TAIR 10
Match: AT2G46170.1 (Reticulon family protein )

HSP 1 Score: 270.0 bits (689), Expect = 1.8e-72
Identity = 141/241 (58.51%), Postives = 182/241 (75.52%), Query Frame = 0


Query: 9   DSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLGGGKPADVF 68
           +S+++KIS+KI    SSSSS+S+        AVK++++R+FGR+KPVH VLGGGKPADVF
Sbjct: 13  ESLMEKISEKIHHHDSSSSSESEYEKPDSPSAVKAKIYRMFGREKPVHKVLGGGKPADVF 72

Query: 69  LWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANTFINKTPPQ 128
           LWR+KK S  +LGVATA+W++FEL+EY L++L+CH+ IL L  +FLWS A+T INKT PQ
Sbjct: 73  LWRDKKLSGAVLGVATAIWVLFELVEYHLLSLLCHISILALGGLFLWSNAHTLINKTSPQ 132

Query: 129 IPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLSVVGT---- 188
           IP++ +PE  FL   S+LR E+N+A  ILR IA GRDLKKFL V +GLW++SVVG     
Sbjct: 133 IPEIHVPEEAFLVVASSLRNELNQAFVILRSIALGRDLKKFLMVVVGLWIISVVGNWFNF 192

Query: 189 -------IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIPIGALQEKK 239
                   V+LHTVP+ YEK+ED+VDPLAEKA  E +KQY VFD KVLSKIPI +L+ K 
Sbjct: 193 LTLVYICFVILHTVPMLYEKHEDKVDPLAEKAMKELQKQYVVFDEKVLSKIPIASLKAKA 252

[Close]

BLAST of Carg20366-RA vs. TAIR 10
Match: AT4G11220.1 (VIRB2-interacting protein 2 )

HSP 1 Score: 269.6 bits (688), Expect = 2.4e-72
Identity = 141/249 (56.63%), Postives = 185/249 (74.30%), Query Frame = 0


Query: 7   ERDSVIDKISDKI---GDCISSSSSDSDD---GLHSKIDAVKSRVFRLFGRDKPVHTVLG 66
           ER+S+++K+S+KI   GD  SSSSSD ++      S   ++KS+V+RLFGR++PVH VLG
Sbjct: 22  ERESLMEKLSEKIHHKGDSSSSSSSDDENEKKSSSSSPKSLKSKVYRLFGRERPVHKVLG 81

Query: 67  GGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANT 126
           GGKPAD+F+W++KK S G+ G AT  W++FEL+EY L+TL+CH++I+ LA++FLWS A  
Sbjct: 82  GGKPADIFMWKDKKMSGGVFGGATVAWVLFELMEYHLLTLLCHVMIVALAVLFLWSNATM 141

Query: 127 FINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLS 186
           FI+K+PP+IP++ +PE   LQ  S LRIEINR IS LR IA GRD+KKFLS   GLWVLS
Sbjct: 142 FIHKSPPKIPEVHIPEEPLLQLASGLRIEINRGISSLREIASGRDIKKFLSAIAGLWVLS 201

Query: 187 VVG-----------TIVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIP 239
           ++G            +VLL TVP+FY+KYED+VD   EKA  E KKQYAV DAKV SKIP
Sbjct: 202 ILGGCYSFLTLAYIALVLLFTVPLFYDKYEDKVDSYGEKAMAELKKQYAVLDAKVFSKIP 261

[Close]

BLAST of Carg20366-RA vs. TAIR 10
Match: AT3G61560.1 (Reticulon family protein )

HSP 1 Score: 266.2 bits (679), Expect = 2.6e-71
Identity = 138/241 (57.26%), Postives = 180/241 (74.69%), Query Frame = 0


Query: 7   ERDSVIDKISDKIGDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTVLGGGKPAD 66
           + +S+++KI+DKI D  SSSSSDS+        A+K++++RLFGR+KPVH VLGGG PAD
Sbjct: 11  KEESLMEKIADKIHDHDSSSSSDSEHEKPESPSALKAKIYRLFGREKPVHKVLGGGLPAD 70

Query: 67  VFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYANTFINKTP 126
           VFLWR+KK SA +LGVATA+W++FEL+EY  ++LVCH+LI  LA +FL S A+ F+NKTP
Sbjct: 71  VFLWRDKKLSASVLGVATAIWVLFELVEYHFLSLVCHILIFALAALFLLSNAHAFMNKTP 130

Query: 127 PQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWVLSVVGT-- 186
           P+IP++ + E  FL  VSALR E+N+A  ILR IA GRDLKKFL V  GLW++SVVG   
Sbjct: 131 PKIPEIHIKEEHFLMIVSALRNELNQAFVILRSIALGRDLKKFLMVVFGLWIISVVGNWF 190

Query: 187 ---------IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVLSKIPIGALQE 237
                     V+LHTVP+ YEK+ED+VDP+AEK   E KK Y VFD KVLSK+P+ +L+ 
Sbjct: 191 NFLTLVYICFVVLHTVPMLYEKHEDKVDPVAEKTLKELKKHYMVFDEKVLSKLPVASLKA 250

[Close]

BLAST of Carg20366-RA vs. TAIR 10
Match: AT1G64090.1 (Reticulan like protein B3 )

HSP 1 Score: 256.5 bits (654), Expect = 2.1e-68
Identity = 143/257 (55.64%), Postives = 182/257 (70.82%), Query Frame = 0


Query: 1   MADQPRERDSVIDKISDKI--GDCISSSSSDSDDGLHSKIDAVKSRVFRLFGRDKPVHTV 60
           MA++ +  +S+++KIS+KI   D  SSSSSDSDD  +S   ++K++++RLFGR++P+H +
Sbjct: 1   MAEEHKHEESIMEKISEKIHGHDDSSSSSSDSDDDKNSA--SLKTKIYRLFGREQPLHKL 60

Query: 61  LGGGKPADVFLWRNKKNSAGILGVATALWIVFELIEYQLITLVCHLLILLLAIMFLWSYA 120
            GGGKPAD+FLWRNKK S G+LG AT  WI+FEL+EY L+TL  H+ IL LA++FLWS A
Sbjct: 61  FGGGKPADIFLWRNKKVSGGVLGAATVSWILFELLEYNLLTLFGHISILALAVLFLWSSA 120

Query: 121 NTFINKTPPQIPDMKLPEVCFLQFVSALRIEINRAISILRGIACGRDLKKFLSVFLGLWV 180
           +TFI+K+P  IP++ +PE   LQ  S LRIEINR  ++LR IA GRDLKKFL V  GLWV
Sbjct: 121 STFIHKSPLHIPEVHIPEDVVLQLASGLRIEINRGFTVLRDIASGRDLKKFLLVIAGLWV 180

Query: 181 LSVVGT-----------IVLLHTVPVFYEKYEDQVDPLAEKAWIEFKKQYAVFDAKVL-- 240
           LS VG+            VLL T+PV YEKYED+VD   EKA  E KKQY  FD KVL  
Sbjct: 181 LSKVGSSCNFLTLIYIATVLLFTIPVLYEKYEDKVDDFGEKAMREIKKQYVEFDVKVLSK 240

The following BLAST results are available for this feature:

BLAST of Carg20366-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013283.1	2.9e-128	100.00	Reticulon-like protein B5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022945796.1	3.0e-125	95.22	reticulon-like protein B1 [Cucurbita moschata]	[more]
XP_022968328.1	8.6e-125	94.82	reticulon-like protein B1 [Cucurbita maxima]	[more]
XP_023541342.1	1.5e-124	94.82	reticulon-like protein B1 [Cucurbita pepo subsp. pepo]	[more]
KAG6574214.1	5.6e-116	94.87	Reticulon-like protein B5, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg20366-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SUR3	1.5e-74	58.89	Reticulon-like protein B1 OS=Arabidopsis thaliana OX=3702 GN=RTNLB1 PE=1 SV=1	[more]
O82352	2.6e-71	58.51	Reticulon-like protein B5 OS=Arabidopsis thaliana OX=3702 GN=RTNLB5 PE=1 SV=1	[more]
Q9SUT9	3.3e-71	56.63	Reticulon-like protein B2 OS=Arabidopsis thaliana OX=3702 GN=RTNLB2 PE=1 SV=1	[more]
Q6DBN4	3.7e-70	57.26	Reticulon-like protein B6 OS=Arabidopsis thaliana OX=3702 GN=RTNLB6 PE=1 SV=1	[more]
Q9SH59	2.9e-67	55.64	Reticulon-like protein B3 OS=Arabidopsis thaliana OX=3702 GN=RTNLB3 PE=2 SV=1	[more]

BLAST of Carg20366-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G1W3	1.4e-125	95.22	Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111449937 PE=4 SV=1	[more]
A0A6J1HXQ1	4.2e-125	94.82	Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111467592 PE=4 SV=1	[more]
A0A0A0L7V4	2.4e-96	75.90	Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G363160 PE=4 SV=1	[more]
A0A6J1EA75	1.4e-88	73.49	Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430763 PE=4 SV=1	[more]
A0A6J1JBF9	4.1e-88	73.09	Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482938 PE=4 SV=1	[more]

BLAST of Carg20366-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23630.1	1.0e-75	58.89	VIRB2-interacting protein 1	[more]
AT2G46170.1	1.8e-72	58.51	Reticulon family protein	[more]
AT4G11220.1	2.4e-72	56.63	VIRB2-interacting protein 2	[more]
AT3G61560.1	2.6e-71	57.26	Reticulon family protein	[more]
AT1G64090.1	2.1e-68	55.64	Reticulan like protein B3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003388	Reticulon	PFAM	PF02453	Reticulon	coord: 65..209 e-value: 2.4E-42 score: 144.6
IPR003388	Reticulon	PROSITE	PS50845	RETICULON	coord: 64..240 score: 36.307178
None	No IPR available	PANTHER	PTHR10994:SF158	RETICULON-LIKE PROTEIN	coord: 1..238
IPR045064	Reticulon-like protein, plant	PANTHER	PTHR10994	RETICULON	coord: 1..238

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg20366	Carg20366	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20366-RA:1	Carg20366-RA:1	exon
Carg20366-RA:2	Carg20366-RA:2	exon
Carg20366-RA:3	Carg20366-RA:3	exon
Carg20366-RA:4	Carg20366-RA:4	exon
Carg20366-RA:5	Carg20366-RA:5	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20366-RA:five_prime_utr	Carg20366-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20366-RA:cds	Carg20366-RA:cds	CDS
Carg20366-RA:cds	Carg20366-RA:cds_2	CDS
Carg20366-RA:cds	Carg20366-RA:cds_3	CDS
Carg20366-RA:cds	Carg20366-RA:cds_4	CDS
Carg20366-RA:cds	Carg20366-RA:cds_5	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg20366-RA	Carg20366-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009617	response to bacterium
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane