Carg20340-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20340-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	thylakoid lumenal 17.4 kDa protein, chloroplastic
Location	Carg_Chr18: 11825003 .. 11826225 (+)
Sequence length	1060
RNA-Seq Expression	Carg20340-RA
Synteny	Carg20340-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGCATCTAAACTTAGGCTAAAACCCAGTTTTTTTTTTCCAAGGACATCTTGTAATAATCTTGTGAGATCCCATATCGGTTGGGGAGGGGAACGAAACATTCTTTATAAGAATGTAGAACCTTTTAAAACCTTGAGAGTAACTGTGGAAGGAAAAGCCCTAAAAGGACAATATCTGGTAACGGCGGACTTAGATTGTTACAAATGGTATCGATTGGAGAGGGAAACGAAACATCTTCTTTTTTTAATATTCTTCCTTTTATATGTTCTTCCTTTTATAGTCATGATCAACAAAATTCGTTCACTTTGTGTGGCAAGTGTGAAACAACTCATTATATTTCACCTCGAGAAATAAACCTGTTGAACTCATAATATGATATGTGGGACTTGCAGAGGCTTCCTCCACTGTCGACAGAACCCAACCGATGTGAGCGTGCTTTTGTTGGCAACACTATAGGTCAAGCAAATGGTGTGTATGATAAGCCAATTGATCTTCGTTTCTGCGACTACACAAATGATAAAAGCCAGTTAAAAGGGAAATCTCTTGCTGCTGCCTTGATGTCAGAAGCAAAGTTTGATGGTGCAGACCTCTCTGAAGTGGTGATGTCAAAAGCATATGCTGTTGGAGCCAGCTTCAAGGGTAAAATTCTCTCTTTCTTACCCCAACTCCAATAGCCAGGCGGATTGTGGACAAAGGAGAAAGACTTTGATTGGATCTCCCAACTCTAAAATCATGTATTTGCCTACAAATATTAATACTCATAACACGGGTTCAAACGTCATGATAACATTGCATATTGCAGGTGTCGATTTCTCGAACGCAGTTCTAGATAGGGTAAATTTTGGGAAAGCCAATCTCCAAGGAGCTTTGTTCAAGAACACTGTACTTTCAGGGTCCACATTTGATGATGCTCAATTACAAGATGCAGTTTTTGAGGACACAATCATAGGCTACATTGATCTTCAGAAACTGTGTGTAAATCCAACTATTAGTCCTGAAGGAAGAGCTGAATTGGGATGTCGATGATTTCAATATAGTCTGTGTATGTATTGTATTGTATTGTCTAGACAACTTAATGATTTTGGTAAGTAATACATATTCAAAGAGACAACTCCCTCTAACTTCTGTTGAAGAACAATGTGGTGTCACTGTCAAGTTCATGTTTTCCGTAATCTTCTTTCGTTGTTTCCTTAGGAACAAAATCTGATGTTTTTTCTTTTGAGT

mRNA sequence

TTGCATCTAAACTTAGGCTAAAACCCAGTTTTTTTTTTCCAAGGACATCTTGTAATAATCTTGTGAGATCCCATATCGGTTGGGGAGGGGAACGAAACATTCTTTATAAGAATGTAGAACCTTTTAAAACCTTGAGAGTAACTGTGGAAGGAAAAGCCCTAAAAGGACAATATCTGGTAACGGCGGACTTAGATTGTTACAAATGGTATCGATTGGAGAGGGAAACGAAACATCTTCTTTTTTTAATATTCTTCCTTTTATATGTTCTTCCTTTTATAGTCATGATCAACAAAATTCGTTCACTTTGTGTGGCAAGTGTGAAACAACTCATTATATTTCACCTCGAGAAATAAACCTGTTGAACTCATAATATGATATGTGGGACTTGCAGAGGCTTCCTCCACTGTCGACAGAACCCAACCGATGTGAGCGTGCTTTTGTTGGCAACACTATAGGTCAAGCAAATGGTGTGTATGATAAGCCAATTGATCTTCGTTTCTGCGACTACACAAATGATAAAAGCCAGTTAAAAGGGAAATCTCTTGCTGCTGCCTTGATGTCAGAAGCAAAGTTTGATGGTGCAGACCTCTCTGAAGTGGTGATGTCAAAAGCATATGCTGTTGGAGCCAGCTTCAAGGGTGTCGATTTCTCGAACGCAGTTCTAGATAGGGTAAATTTTGGGAAAGCCAATCTCCAAGGAGCTTTGTTCAAGAACACTGTACTTTCAGGGTCCACATTTGATGATGCTCAATTACAAGATGCAGTTTTTGAGGACACAATCATAGGCTACATTGATCTTCAGAAACTGTGTGTAAATCCAACTATTAGTCCTGAAGGAAGAGCTGAATTGGGATGTCGATGATTTCAATATAGTCTGTGTATGTATTGTATTGTATTGTCTAGACAACTTAATGATTTTGGTAAGTAATACATATTCAAAGAGACAACTCCCTCTAACTTCTGTTGAAGAACAATGTGGTGTCACTGTCAAGTTCATGTTTTCCGTAATCTTCTTTCGTTGTTTCCTTAGGAACAAAATCTGATGTTTTTTCTTTTGAGT

Coding sequence (CDS)

ATGTGGGACTTGCAGAGGCTTCCTCCACTGTCGACAGAACCCAACCGATGTGAGCGTGCTTTTGTTGGCAACACTATAGGTCAAGCAAATGGTGTGTATGATAAGCCAATTGATCTTCGTTTCTGCGACTACACAAATGATAAAAGCCAGTTAAAAGGGAAATCTCTTGCTGCTGCCTTGATGTCAGAAGCAAAGTTTGATGGTGCAGACCTCTCTGAAGTGGTGATGTCAAAAGCATATGCTGTTGGAGCCAGCTTCAAGGGTGTCGATTTCTCGAACGCAGTTCTAGATAGGGTAAATTTTGGGAAAGCCAATCTCCAAGGAGCTTTGTTCAAGAACACTGTACTTTCAGGGTCCACATTTGATGATGCTCAATTACAAGATGCAGTTTTTGAGGACACAATCATAGGCTACATTGATCTTCAGAAACTGTGTGTAAATCCAACTATTAGTCCTGAAGGAAGAGCTGAATTGGGATGTCGATGA

Protein sequence

MWDLQRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEAKFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDAQLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR

Homology

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BLAST of Carg20340-RA vs. NCBI nr
Match: KAG7013309.1 (Thylakoid lumenal 17.4 kDa protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 327.0 bits (837), Expect = 9.0e-86
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MWDLQRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAAL 60
           MWDLQRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAAL
Sbjct: 1   MWDLQRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAAL 60

Query: 61  MSEAKFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGST 120
           MSEAKFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGST
Sbjct: 61  MSEAKFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGST 120

Query: 121 FDDAQLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
           FDDAQLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR
Sbjct: 121 FDDAQLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 161

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BLAST of Carg20340-RA vs. NCBI nr
Match: XP_022945269.1 (thylakoid lumenal 17.4 kDa protein, chloroplastic [Cucurbita moschata])

HSP 1 Score: 316.6 bits (810), Expect = 1.2e-82
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA
Sbjct: 81  QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 140

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA
Sbjct: 141 KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 200

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
           QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR
Sbjct: 201 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 237

[Close]

BLAST of Carg20340-RA vs. NCBI nr
Match: KAG6574243.1 (Thylakoid lumenal 17.4 kDa protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 315.1 bits (806), Expect = 3.5e-82
Identity = 156/157 (99.36%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTN+KSQLKGKSLAAALMSEA
Sbjct: 81  QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNEKSQLKGKSLAAALMSEA 140

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA
Sbjct: 141 KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 200

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
           QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR
Sbjct: 201 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 237

[Close]

BLAST of Carg20340-RA vs. NCBI nr
Match: XP_022984509.1 (thylakoid lumenal 17.4 kDa protein, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 315.1 bits (806), Expect = 3.5e-82
Identity = 156/157 (99.36%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTN+KSQLKGKSLAAALMSEA
Sbjct: 121 QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNEKSQLKGKSLAAALMSEA 180

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA
Sbjct: 181 KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 240

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
           QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR
Sbjct: 241 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 277

[Close]

BLAST of Carg20340-RA vs. NCBI nr
Match: XP_022968163.1 (thylakoid lumenal 17.4 kDa protein, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 313.9 bits (803), Expect = 7.9e-82
Identity = 155/157 (98.73%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTN+KSQLKGKSLAAALMSEA
Sbjct: 81  QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNEKSQLKGKSLAAALMSEA 140

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTV+SGSTFDDA
Sbjct: 141 KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVVSGSTFDDA 200

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
           QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR
Sbjct: 201 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 237

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BLAST of Carg20340-RA vs. ExPASy Swiss-Prot
Match: P81760 (Thylakoid lumenal 17.4 kDa protein, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TL17 PE=1 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 3.6e-69
Identity = 124/157 (78.98%), Postives = 146/157 (92.99%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEP+RCE+AFVGNTIGQANGVYDKP+DLRFCDYTND++ LKGK+L+AALM  A
Sbjct: 80  QRLPPLSTEPDRCEKAFVGNTIGQANGVYDKPLDLRFCDYTNDQTNLKGKTLSAALMVGA 139

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGAD++EVVMSKAYAV ASFKGV+F+NAV+DRVNFGK+NL+GA+F+NTVLSGSTF++A
Sbjct: 140 KFDGADMTEVVMSKAYAVEASFKGVNFTNAVIDRVNFGKSNLKGAVFRNTVLSGSTFEEA 199

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
            L+D VFEDTIIGYIDLQK+C N +I+ EGR  LGCR
Sbjct: 200 NLEDVVFEDTIIGYIDLQKICRNESINEEGRLVLGCR 236

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BLAST of Carg20340-RA vs. ExPASy Swiss-Prot
Match: Q8H1Q1 (Thylakoid lumenal protein TL20.3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TL20.3 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.7e-10
Identity = 46/157 (29.30%), Postives = 72/157 (45.86%), Query Frame = 0


Query: 9   PLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEAKFDG 68
           P   E NR E A      G  +       DL    ++N+    +  +  +A M E+ F G
Sbjct: 87  PAMAELNRFE-ADTRGEFGIGSAAQYGSADLSKTVHSNE--NFRRANFTSADMRESDFSG 146

Query: 69  ADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDAQLQD 128
           +  +   + KA A  A+F G D S+ ++DR+   +ANL  A+   +VL+ S    A+++ 
Sbjct: 147 STFNGAYLEKAVAYKANFSGADLSDTLMDRMVLNEANLTNAVLVRSVLTRSDLGGAKIEG 206

Query: 129 AVFEDTIIGYIDLQKLC-----VNPTISPEGRAELGC 161
           A F D +I  +  Q LC      NP    + R  LGC
Sbjct: 207 ADFSDAVIDLLQKQALCKYATGTNPLTGVDTRKSLGC 240

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BLAST of Carg20340-RA vs. ExPASy Swiss-Prot
Match: B1WVN5 (Pentapeptide repeat protein Rfr32 OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=rfr32 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 3.9e-07
Identity = 35/119 (29.41%), Postives = 57/119 (47.90%), Query Frame = 0


Query: 53  GKSLAAAL-----MSEAKFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQ 112
           GKSL  A      ++++ F  ADL   V + +  +GA   G D +N +    +F  A+L 
Sbjct: 61  GKSLTYAQFTNADLTDSNFSEADLRGAVFNGSALIGADLHGADLTNGLAYLTSFKGADLT 120

Query: 113 GALFKNTVLSGSTFDDAQLQDAVFEDTIIGYIDLQKLC-----VNPTISPEGRAELGCR 162
            A+    ++  + FDDA++  A F   ++   ++ KLC     VNP      R  LGC+
Sbjct: 121 NAVLTEAIMMRTKFDDAKITGADFSLAVLDVYEVDKLCDRADGVNPKTGVSTRESLGCQ 179

[Close]

BLAST of Carg20340-RA vs. ExPASy Swiss-Prot
Match: P74221 (Uncharacterized protein slr1152 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1152 PE=4 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 4.8e-05
Identity = 31/98 (31.63%), Postives = 52/98 (53.06%), Query Frame = 0


Query: 50  QLKGKSLAAALMSEAKFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGA 109
           Q+    L  A +++A    ADL++  +  A+AV  +FKG   S A L       ANL+GA
Sbjct: 91  QMNWADLTYAKLNQADLSHADLTKASLYGAFAVKTNFKGAKLSGATL-----AHANLRGA 150

Query: 110 LFKNTVLSGSTFDDAQLQDAVFEDTIIGYIDLQKLCVN 148
             + T L+G+    A L++A F+     + +LQ+ C++
Sbjct: 151 NLEQTNLTGANLFAANLREANFQKADFSWANLQEACLS 183

[Close]

BLAST of Carg20340-RA vs. ExPASy TrEMBL
Match: A0A6J1G0I9 (thylakoid lumenal 17.4 kDa protein, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449567 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 5.9e-83
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA
Sbjct: 81  QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 140

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA
Sbjct: 141 KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 200

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
           QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR
Sbjct: 201 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 237

[Close]

BLAST of Carg20340-RA vs. ExPASy TrEMBL
Match: A0A6J1JAQ7 (thylakoid lumenal 17.4 kDa protein, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482783 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.7e-82
Identity = 156/157 (99.36%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTN+KSQLKGKSLAAALMSEA
Sbjct: 121 QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNEKSQLKGKSLAAALMSEA 180

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA
Sbjct: 181 KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 240

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
           QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR
Sbjct: 241 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 277

[Close]

BLAST of Carg20340-RA vs. ExPASy TrEMBL
Match: A0A6J1HX78 (thylakoid lumenal 17.4 kDa protein, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111467480 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.8e-82
Identity = 155/157 (98.73%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTN+KSQLKGKSLAAALMSEA
Sbjct: 81  QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNEKSQLKGKSLAAALMSEA 140

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTV+SGSTFDDA
Sbjct: 141 KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVVSGSTFDDA 200

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
           QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR
Sbjct: 201 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 237

[Close]

BLAST of Carg20340-RA vs. ExPASy TrEMBL
Match: A0A6J1E548 (thylakoid lumenal 17.4 kDa protein, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430851 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.8e-82
Identity = 155/157 (98.73%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTN+KSQLKGKSLAAALMSEA
Sbjct: 125 QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNEKSQLKGKSLAAALMSEA 184

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA
Sbjct: 185 KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 244

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
           +LQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR
Sbjct: 245 ELQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 281

[Close]

BLAST of Carg20340-RA vs. ExPASy TrEMBL
Match: A0A5D3CJC6 (Thylakoid lumenal 17.4 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001360 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 8.5e-82
Identity = 154/157 (98.09%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTN+K+QLKGKSLAAALMS+A
Sbjct: 81  QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNEKNQLKGKSLAAALMSDA 140

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA
Sbjct: 141 KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 200

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
           QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR
Sbjct: 201 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 237

[Close]

BLAST of Carg20340-RA vs. TAIR 10
Match: AT5G53490.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 2.5e-70
Identity = 124/157 (78.98%), Postives = 146/157 (92.99%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEP+RCE+AFVGNTIGQANGVYDKP+DLRFCDYTND++ LKGK+L+AALM  A
Sbjct: 79  QRLPPLSTEPDRCEKAFVGNTIGQANGVYDKPLDLRFCDYTNDQTNLKGKTLSAALMVGA 138

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGAD++EVVMSKAYAV ASFKGV+F+NAV+DRVNFGK+NL+GA+F+NTVLSGSTF++A
Sbjct: 139 KFDGADMTEVVMSKAYAVEASFKGVNFTNAVIDRVNFGKSNLKGAVFRNTVLSGSTFEEA 198

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
            L+D VFEDTIIGYIDLQK+C N +I+ EGR  LGCR
Sbjct: 199 NLEDVVFEDTIIGYIDLQKICRNESINEEGRLVLGCR 235

[Close]

BLAST of Carg20340-RA vs. TAIR 10
Match: AT5G53490.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 2.5e-70
Identity = 124/157 (78.98%), Postives = 146/157 (92.99%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEP+RCE+AFVGNTIGQANGVYDKP+DLRFCDYTND++ LKGK+L+AALM  A
Sbjct: 80  QRLPPLSTEPDRCEKAFVGNTIGQANGVYDKPLDLRFCDYTNDQTNLKGKTLSAALMVGA 139

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGAD++EVVMSKAYAV ASFKGV+F+NAV+DRVNFGK+NL+GA+F+NTVLSGSTF++A
Sbjct: 140 KFDGADMTEVVMSKAYAVEASFKGVNFTNAVIDRVNFGKSNLKGAVFRNTVLSGSTFEEA 199

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
            L+D VFEDTIIGYIDLQK+C N +I+ EGR  LGCR
Sbjct: 200 NLEDVVFEDTIIGYIDLQKICRNESINEEGRLVLGCR 236

[Close]

BLAST of Carg20340-RA vs. TAIR 10
Match: AT5G53490.3 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 2.5e-70
Identity = 124/157 (78.98%), Postives = 146/157 (92.99%), Query Frame = 0


Query: 5   QRLPPLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEA 64
           QRLPPLSTEP+RCE+AFVGNTIGQANGVYDKP+DLRFCDYTND++ LKGK+L+AALM  A
Sbjct: 94  QRLPPLSTEPDRCEKAFVGNTIGQANGVYDKPLDLRFCDYTNDQTNLKGKTLSAALMVGA 153

Query: 65  KFDGADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDA 124
           KFDGAD++EVVMSKAYAV ASFKGV+F+NAV+DRVNFGK+NL+GA+F+NTVLSGSTF++A
Sbjct: 154 KFDGADMTEVVMSKAYAVEASFKGVNFTNAVIDRVNFGKSNLKGAVFRNTVLSGSTFEEA 213

Query: 125 QLQDAVFEDTIIGYIDLQKLCVNPTISPEGRAELGCR 162
            L+D VFEDTIIGYIDLQK+C N +I+ EGR  LGCR
Sbjct: 214 NLEDVVFEDTIIGYIDLQKICRNESINEEGRLVLGCR 250

[Close]

BLAST of Carg20340-RA vs. TAIR 10
Match: AT1G12250.1 (Pentapeptide repeat-containing protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-11
Identity = 46/157 (29.30%), Postives = 72/157 (45.86%), Query Frame = 0


Query: 9   PLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEAKFDG 68
           P   E NR E A      G  +       DL    ++N+    +  +  +A M E+ F G
Sbjct: 87  PAMAELNRFE-ADTRGEFGIGSAAQYGSADLSKTVHSNE--NFRRANFTSADMRESDFSG 146

Query: 69  ADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDAQLQD 128
           +  +   + KA A  A+F G D S+ ++DR+   +ANL  A+   +VL+ S    A+++ 
Sbjct: 147 STFNGAYLEKAVAYKANFSGADLSDTLMDRMVLNEANLTNAVLVRSVLTRSDLGGAKIEG 206

Query: 129 AVFEDTIIGYIDLQKLC-----VNPTISPEGRAELGC 161
           A F D +I  +  Q LC      NP    + R  LGC
Sbjct: 207 ADFSDAVIDLLQKQALCKYATGTNPLTGVDTRKSLGC 240

[Close]

BLAST of Carg20340-RA vs. TAIR 10
Match: AT1G12250.2 (Pentapeptide repeat-containing protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-11
Identity = 46/157 (29.30%), Postives = 72/157 (45.86%), Query Frame = 0


Query: 9   PLSTEPNRCERAFVGNTIGQANGVYDKPIDLRFCDYTNDKSQLKGKSLAAALMSEAKFDG 68
           P   E NR E A      G  +       DL    ++N+    +  +  +A M E+ F G
Sbjct: 13  PAMAELNRFE-ADTRGEFGIGSAAQYGSADLSKTVHSNE--NFRRANFTSADMRESDFSG 72

Query: 69  ADLSEVVMSKAYAVGASFKGVDFSNAVLDRVNFGKANLQGALFKNTVLSGSTFDDAQLQD 128
           +  +   + KA A  A+F G D S+ ++DR+   +ANL  A+   +VL+ S    A+++ 
Sbjct: 73  STFNGAYLEKAVAYKANFSGADLSDTLMDRMVLNEANLTNAVLVRSVLTRSDLGGAKIEG 132

Query: 129 AVFEDTIIGYIDLQKLC-----VNPTISPEGRAELGC 161
           A F D +I  +  Q LC      NP    + R  LGC
Sbjct: 133 ADFSDAVIDLLQKQALCKYATGTNPLTGVDTRKSLGC 166

The following BLAST results are available for this feature:

BLAST of Carg20340-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013309.1	9.0e-86	100.00	Thylakoid lumenal 17.4 kDa protein, chloroplastic [Cucurbita argyrosperma subsp....	[more]
XP_022945269.1	1.2e-82	100.00	thylakoid lumenal 17.4 kDa protein, chloroplastic [Cucurbita moschata]	[more]
KAG6574243.1	3.5e-82	99.36	Thylakoid lumenal 17.4 kDa protein, chloroplastic, partial [Cucurbita argyrosper...	[more]
XP_022984509.1	3.5e-82	99.36	thylakoid lumenal 17.4 kDa protein, chloroplastic-like [Cucurbita maxima]	[more]
XP_022968163.1	7.9e-82	98.73	thylakoid lumenal 17.4 kDa protein, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Carg20340-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P81760	3.6e-69	78.98	Thylakoid lumenal 17.4 kDa protein, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q8H1Q1	1.7e-10	29.30	Thylakoid lumenal protein TL20.3, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
B1WVN5	3.9e-07	29.41	Pentapeptide repeat protein Rfr32 OS=Crocosphaera subtropica (strain ATCC 51142 ...	[more]
P74221	4.8e-05	31.63	Uncharacterized protein slr1152 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ...	[more]

BLAST of Carg20340-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G0I9	5.9e-83	100.00	thylakoid lumenal 17.4 kDa protein, chloroplastic OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1JAQ7	1.7e-82	99.36	thylakoid lumenal 17.4 kDa protein, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1HX78	3.8e-82	98.73	thylakoid lumenal 17.4 kDa protein, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1E548	3.8e-82	98.73	thylakoid lumenal 17.4 kDa protein, chloroplastic-like OS=Cucurbita moschata OX=...	[more]
A0A5D3CJC6	8.5e-82	98.09	Thylakoid lumenal 17.4 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Carg20340-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53490.2	2.5e-70	78.98	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]
AT5G53490.1	2.5e-70	78.98	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]
AT5G53490.3	2.5e-70	78.98	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]
AT1G12250.1	1.2e-11	29.30	Pentapeptide repeat-containing protein	[more]
AT1G12250.2	1.2e-11	29.30	Pentapeptide repeat-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001646	Pentapeptide repeat	PFAM	PF13599	Pentapeptide_4	coord: 84..142 e-value: 1.3E-8 score: 34.9
None	No IPR available	GENE3D	2.160.20.80		coord: 37..161 e-value: 2.2E-22 score: 80.6
None	No IPR available	PANTHER	PTHR47485:SF1	THYLAKOID LUMENAL 17.4 KDA PROTEIN, CHLOROPLASTIC	coord: 4..161
None	No IPR available	SUPERFAMILY	141571	Pentapeptide repeat-like	coord: 39..143
IPR044195	Pentapeptide repeat containing protein TL17-like	PANTHER	PTHR47485	THYLAKOID LUMENAL 17.4 KDA PROTEIN, CHLOROPLASTIC	coord: 4..161

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg20340	Carg20340	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20340-RA:2	Carg20340-RA:2	exon
Carg20340-RA:1	Carg20340-RA:1	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20340-RA:five_prime_utr	Carg20340-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20340-RA:cds	Carg20340-RA:cds	CDS
Carg20340-RA:cds	Carg20340-RA:cds_2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg20340-RA:three_prime_utr	Carg20340-RA:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg20340-RA	Carg20340-RA-protein	polypeptide